Construction of an economical xylose-utilizing Saccharomyces cerevisiae and its ethanol fermentation.

IF 2.4 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

FEMS yeast research Pub Date : 2024-01-09 DOI:10.1093/femsyr/foae001

Fan Li, Wenxin Bai, Yuan Zhang, Zijian Zhang, Deguo Zhang, Naidong Shen, Jingwei Yuan, Guomiao Zhao, Xiaoyan Wang

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引用次数: 0

Abstract

Traditional industrial Saccharomyces cerevisiae could not metabolize xylose due to the lack of a specific enzyme system for the reaction from xylose to xylulose. This study aims to metabolically remould industrial S. cerevisiae for the purpose of utilizing both glucose and xylose with high efficiency. Heterologous gene xylA from Piromyces and homologous genes related to xylose utilization were selected to construct expression cassettes and integrated into genome. The engineered strain was domesticated with industrial material under optimizing conditions subsequently to further improve xylose utilization rates. The resulting S. cerevisiae strain ABX0928-0630 exhibits a rapid growth rate and possesses near 100% xylose utilization efficiency to produce ethanol with industrial material. Pilot-scale fermentation indicated the predominant feature of ABX0928-0630 for industrial application, with ethanol yield of 0.48 g/g sugars after 48 hours and volumetric xylose consumption rate of 0.87 g/l/h during the first 24 hours. Transcriptome analysis during the modification and domestication process revealed a significant increase in the expression level of pathways associated with sugar metabolism and sugar sensing. Meanwhile, genes related to glycerol lipid metabolism exhibited a pattern of initial increase followed by a subsequent decrease, providing a valuable reference for the construction of efficient xylose-fermenting strains.

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构建经济型木糖利用酿酒酵母菌及其乙醇发酵。

由于缺乏将木糖转化为木酮糖的特定酶系统，传统的工业酿酒酵母无法代谢木糖。本研究旨在对工业化酿酒酵母进行新陈代谢改造，以高效利用葡萄糖和木糖。研究人员选择了来自轮枝菌的异源基因 xylA 和与木糖利用相关的同源基因构建表达盒，并将其整合到基因组中。随后，在优化条件下用工业原料驯化工程菌株，以进一步提高木糖利用率。所获得的 S. cerevisiae 菌株 ABX0928-0630 生长迅速，木糖利用率接近 100%，可与工业原料一起生产乙醇。中试规模发酵表明了 ABX0928-0630 在工业应用方面的主要特点，48 小时后乙醇产量为 0.48 克/克糖，前 24 小时木糖体积消耗率为 0.87 克/升-1 小时-1。在改良和驯化过程中进行的转录组分析表明，与糖代谢和糖传感相关的通路的表达水平显著提高。同时，与甘油脂代谢相关的基因表现出先增加后减少的模式，这为构建高效木糖发酵菌株提供了宝贵的参考。

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来源期刊

FEMS yeast research 生物-生物工程与应用微生物

CiteScore

5.70

自引率

6.20%

发文量

审稿时长

1 months

期刊介绍： FEMS Yeast Research offers efficient publication of high-quality original Research Articles, Mini-reviews, Letters to the Editor, Perspectives and Commentaries that express current opinions. The journal will select for publication only those manuscripts deemed to be of major relevance to the field and generally will not consider articles that are largely descriptive without insights on underlying mechanism or biology. Submissions on any yeast species are welcome provided they report results within the scope outlined below and are of significance to the yeast field.