Expert Review of Clinical Immunology最新文献_第7页

Thyroid eye disease in the biologic era: a 40-year paradigm shift in nonsurgical therapeutic strategies. 生物时代的甲状腺眼病：40年来非手术治疗策略的范式转变

IF 3.9 3区医学

Expert Review of Clinical Immunology Pub Date : 2025-06-01 Epub Date: 2025-05-25 DOI: 10.1080/1744666X.2025.2509582

Erqian Wang, Zuyi Yang, Zhixuan Xie, Dianzhe Tian, Haiyan Xu, Hui Li, Youxin Chen

{"title":"Thyroid eye disease in the biologic era: a 40-year paradigm shift in nonsurgical therapeutic strategies.","authors":"Erqian Wang, Zuyi Yang, Zhixuan Xie, Dianzhe Tian, Haiyan Xu, Hui Li, Youxin Chen","doi":"10.1080/1744666X.2025.2509582","DOIUrl":"10.1080/1744666X.2025.2509582","url":null,"abstract":"Background: Over the last four decades, there has been a progressive increase in the number of publications and citations on research related to thyroid-associated ophthalmopathy (TAO) nonsurgical treatment across many countries/regions, institutions, and authors, with a special focus on biological immunotherapy.Research design and methods: Examing 1600 publications collected from the Web of Science Core Collection database on TAO research from 1983 to 2023, our bibliometric analysis evaluated various bibliometric indicators, among which some important subtopics were identified and further discussed and reviewed.Results: The study showed that novel insights into the pathogenesis of TAO and new immunological targets for nonsurgical treatments were the major research focus over the past 40 years. Especially, targeted biological immunotherapies were on the rise, promoting treatments efficacy and patients' quality of life.Conclusions: Our study provided a thorough overview and visual presentation of the evolutionary landscape and emerging frontiers for nonsurgical treatment of TAO surrounding its immunological mechanism and therapeutic strategy. It also shed light on its global collaboration patterns, current trends and research hotspots, hopefully to facilitate collaborative initiatives and guiding future research.","PeriodicalId":12175,"journal":{"name":"Expert Review of Clinical Immunology","volume":" ","pages":"787-802"},"PeriodicalIF":3.9,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144119303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlation of serum Helios, CD226, TIGIT, and Foxp3 with tear film osmotic pressure and dry eye disease in patients with rheumatoid arthritis. 类风湿性关节炎患者血清Helios、CD226、tigit和Foxp3与泪膜渗透压和干眼症的相关性

IF 3.9 3区医学

Expert Review of Clinical Immunology Pub Date : 2025-06-01 Epub Date: 2025-06-16 DOI: 10.1080/1744666X.2025.2512451

Lijing Huang, Peilin Cheng, Zheli Niu, Liping Zang, Zicong Chen, Chunchun Yang, Wenhua Ma, Wenjia Nie

{"title":"Correlation of serum Helios, CD226, TIGIT, and Foxp3 with tear film osmotic pressure and dry eye disease in patients with rheumatoid arthritis.","authors":"Lijing Huang, Peilin Cheng, Zheli Niu, Liping Zang, Zicong Chen, Chunchun Yang, Wenhua Ma, Wenjia Nie","doi":"10.1080/1744666X.2025.2512451","DOIUrl":"10.1080/1744666X.2025.2512451","url":null,"abstract":"Objective: This study investigated the correlation between serum Helios, CD226, TIGIT, and Foxp3 levels and tear film osmotic pressure in individuals with rheumatoid arthritis (RA) and dry eye disease.Methods: This case-control study enrolled 100 RA patients with dry eye and 100 healthy controls from May 2021 to June 2022. mRNA expression of target genes was quantified using quantitative real-time PCR (qRT-PCR). Tear film osmolality was measured with a commercial osmometer. Statistical analyses included Pearson correlation and multivariate logistic regression.Results: Compared with controls, RA patients exhibited significantly higher mRNA levels of Helios, CD226, TIGIT, and Foxp3 (p < 0.05), along with elevated mean tear osmolarity (312.5 ± 12.3 vs. 295.4 ± 10.8 milliosmoles per liter (mOsm/L), p < 0.05). Notably, 68% of RA patients exceeded the 308 mOsm/L diagnostic threshold versus 22% controls (p < 0.05). Helios (r = 0.62) and Foxp3 (r = 0.58) correlated positively with osmotic pressure, while TIGIT showed a negative correlation (r = -0.49, p < 0.05). Logistic regression revealed that elevated levels of Helios (OR = 2.1, 95% CI: 1.4-3.2), CD226 (OR = 1.8, 95% CI: 1.2-2.7), and Foxp3 (OR = 1.9, 95% CI: 1.3-2.8) were associated with increased dry eye risk (p < 0.05).Conclusion: These immune markers demonstrate significant associations with tear film instability in RA, serving as potential biomarkers for ocular comorbidity monitoring.","PeriodicalId":12175,"journal":{"name":"Expert Review of Clinical Immunology","volume":" ","pages":"815-823"},"PeriodicalIF":3.9,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144180022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

AQP9 weakens the cytotoxicity of CD8⁺ T cells in colon adenocarcinoma by boosting M2 polarization of macrophages under hypoxia conditions. 在缺氧条件下，AQP9通过促进巨噬细胞M2极化，减弱CD8+ T细胞在结肠腺癌中的细胞毒性。

IF 3.9 3区医学

Expert Review of Clinical Immunology Pub Date : 2025-06-01 Epub Date: 2025-05-08 DOI: 10.1080/1744666X.2025.2501718

Jinping Chen, Zongda Cai, Shurong Huang, Yangqiang Wang, Shiyang Zhan, Wei Zheng, Pan Chi

{"title":"AQP9 weakens the cytotoxicity of CD8+ T cells in colon adenocarcinoma by boosting M2 polarization of macrophages under hypoxia conditions.","authors":"Jinping Chen, Zongda Cai, Shurong Huang, Yangqiang Wang, Shiyang Zhan, Wei Zheng, Pan Chi","doi":"10.1080/1744666X.2025.2501718","DOIUrl":"10.1080/1744666X.2025.2501718","url":null,"abstract":"Background: Colon adenocarcinoma (COAD) is a leading cause of cancer mortality, with Aquaporin 9 (AQP9) implicated in its progression. M2 macrophages in the tumor microenvironment (TME) promote cancer metastasis, but the role of AQP9 on M2 macrophages remains unelucidated.Research design and methods: Using COAD cell lines, AQP9 expression was analyzed via RT-qPCR and Western blot (WB). Hypoxic conditions were simulated to assess HIF-1α and AQP9 interactions through ChIP and dual-luciferase assays. AQP9 knockdown effects on proliferation/migration were tested via colony formation and wound healing. M2 macrophage polarization and CD8+ T cell cytotoxicity were evaluated using flow cytometry, ELISA, and IHC in co-culture systems.Results: AQP9 was upregulated in COAD and correlated with poor prognosis. After AQP9 in COAD cells was knocked down, the abilities of tumor cells to migrate and proliferate were dampened. Hypoxia upregulated HIF-1α, which transcriptionally activated AQP9. Knocking down AQP9 repressed the M2 polarization of macrophages, thereby reinforcing the cytotoxicity of CD8+ T cells. No adverse events were reported in vitro.Conclusion: AQP9 promotes COAD progression by driving HIF-1α-mediated M2 polarization, impairing CD8+ T cell function. Key limitations include the lack of in vivo validation and clinical cohort analysis.","PeriodicalId":12175,"journal":{"name":"Expert Review of Clinical Immunology","volume":" ","pages":"803-814"},"PeriodicalIF":3.9,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143986221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glucocorticoid in systemic lupus erythematosus: the art beyond science. 系统性红斑狼疮的糖皮质激素：超越科学的艺术。

IF 3.9 3区医学

Expert Review of Clinical Immunology Pub Date : 2025-05-01 Epub Date: 2025-04-18 DOI: 10.1080/1744666X.2025.2494654

Tsz Ching Mok, Chi Chiu Mok

{"title":"Glucocorticoid in systemic lupus erythematosus: the art beyond science.","authors":"Tsz Ching Mok, Chi Chiu Mok","doi":"10.1080/1744666X.2025.2494654","DOIUrl":"10.1080/1744666X.2025.2494654","url":null,"abstract":"Introduction: Glucocorticoid (GC) remains the main stay of treatment for systemic lupus erythematosus (SLE) but is associated with a myriad of untoward effects. On the other hand, withdrawal of maintenance immunosuppression, including low-dose GCs, carries a risk of SLE flare.Areas covered: The molecular mechanisms of GCs and their implications for dosing strategies in clinical practice are discussed. Evidence regarding withdrawal of maintenance immunosuppression in SLE is reviewed.Expert opinions: The initial GC regimens for different manifestations of SLE are heterogeneous, with no major randomized controlled trials (RCTs) on their efficacy and toxicities available. RCTs on withdrawal of immunosuppressive drugs in quiescent SLE are inconsistent but appear to show an increase in disease flares, with risk factors being younger age, renal disease, cessation of hydroxychloroquine, shorter duration of remission, serological activity, and an abrupt tapering regime. The lowest effective doses of GC and immunosuppressive drugs should be adopted, and the decision to withdraw immunosuppression should be individualized. Newer strategies for GC sparing, including combination therapy of immunosuppressive and biological/targeted agents, and the use of methylprednisolone pulses for initial therapy of less serious manifestations of SLE, could ameliorate the toxicities of immunosuppression and help advance to the ultimate target of drug-free remission.","PeriodicalId":12175,"journal":{"name":"Expert Review of Clinical Immunology","volume":" ","pages":"543-553"},"PeriodicalIF":3.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143958473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence of peripheral neuropathy in Egyptian psoriatic arthritis patients and its correlation with disease activity. 埃及银屑病关节炎患者周围神经病变的患病率及其与疾病活动度的相关性

IF 3.9 3区医学

Expert Review of Clinical Immunology Pub Date : 2025-05-01 Epub Date: 2025-04-21 DOI: 10.1080/1744666X.2025.2495170

MennaAllah Mohamed Ali Elmalla, Hanaa Ahmad Samy Elbanna, Manal Shawky Hussein Sayed Ahmed, Amira Mohamed El Sharkawy

{"title":"Prevalence of peripheral neuropathy in Egyptian psoriatic arthritis patients and its correlation with disease activity.","authors":"MennaAllah Mohamed Ali Elmalla, Hanaa Ahmad Samy Elbanna, Manal Shawky Hussein Sayed Ahmed, Amira Mohamed El Sharkawy","doi":"10.1080/1744666X.2025.2495170","DOIUrl":"10.1080/1744666X.2025.2495170","url":null,"abstract":"Introduction: Peripheral neuropathy (PN) may arise from various etiologies, including immune-mediated diseases. This study aimed to detect the prevalence and clinical determinants of PN in psoriatic arthritis (PsA) patients, with or without skin lesions, and to evaluate its correlation with disease activity.Research design and methods: This cross-sectional study included 60 PsA patients and 60 apparently healthy controls. Neuropathic pain was evaluated using the Leeds Assessment of Neuropathic Symptoms and Signs (LANSS) score, while PN was assessed through the Michigan Neuropathy Screening Instrument (MNSI), in conjunction with electrophysiological assessment.Results: The LANSS score demonstrated the capability to predict neuropathic pain in PsA patients with a sensitivity of 93.89% and specificity of 83.33%. Furthermore, the MNSI Questionnaire score revealed a sensitivity of 91.67% and specificity of 77.78% in predicting PN among PsA patients. Carpal tunnel syndrome represented the most prevalent neuropathy identified in 36% of PsA patients, followed by peripheral polyneuropathy in 6% and ulnar neuropathy in 4%.Conclusions: Patients with PsA have higher prevalence of neuropathy, particularly carpal tunnel syndrome, which negatively impacts pain perception, functional capability, and quality of life, particularly in those with higher disease activity suggesting potential association between inflammation and neurological dysfunction.","PeriodicalId":12175,"journal":{"name":"Expert Review of Clinical Immunology","volume":" ","pages":"659-665"},"PeriodicalIF":3.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143998690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Individual differences in response to dust-mite-allergen specific immunotherapy in allergic rhinitis: a meta-analysis of randomized controlled trials. 变应性鼻炎对尘螨过敏原特异性免疫治疗反应的个体差异：随机对照试验的荟萃分析。

IF 3.9 3区医学

Expert Review of Clinical Immunology Pub Date : 2025-05-01 Epub Date: 2025-04-29 DOI: 10.1080/1744666X.2025.2499596

Dandan Fang, Jiajia Wang, Jingyun Li, Luo Zhang, Yuan Zhang

{"title":"Individual differences in response to dust-mite-allergen specific immunotherapy in allergic rhinitis: a meta-analysis of randomized controlled trials.","authors":"Dandan Fang, Jiajia Wang, Jingyun Li, Luo Zhang, Yuan Zhang","doi":"10.1080/1744666X.2025.2499596","DOIUrl":"10.1080/1744666X.2025.2499596","url":null,"abstract":"Introduction: The variability in the efficacy of allergen-specific immunotherapy (AIT) for nasal symptom control can be attributed to individual differences, to explore the hypothesis of systematic variability in AR symptom alleviation with AIT and to determine whether this variability correlates with AR severity, route of administration, treatment duration, age, or study publication year.Methods: We reviewed randomized controlled trials (RCTs) of AIT for dust mite (DM)-induced AR, extracting data on baseline mean, endpoint mean, standard deviation (SD), and participant numbers. A random-slope mixed-effects model (RSMM) was employed to evaluate the differences in variability between the AIT and control groups, as well as to identify potential influencing factors.Results: There was no significant difference in response variability between the AIT and control groups. The response variability to AIT was not associated with AR severity, route of administration, age, or year of publication. The cohort that underwent 36 months of AIT exhibited a higher degree of response variability compared to the group treated for 6 months.Conclusion: The present study did not identify systematic variability in individual response to AIT when measured by TNSS alone. More refined outcome measures and more associated factors are needed to explore personalized AIT in the future.","PeriodicalId":12175,"journal":{"name":"Expert Review of Clinical Immunology","volume":" ","pages":"617-626"},"PeriodicalIF":3.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143971499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Targeting the ubiquitin-proteasome pathway in systemic lupus erythematosus. 靶向系统性红斑狼疮的泛素-蛋白酶体途径。

IF 3.9 3区医学

Expert Review of Clinical Immunology Pub Date : 2025-05-01 Epub Date: 2025-04-24 DOI: 10.1080/1744666X.2025.2497845

Chi Chiu Mok

{"title":"Targeting the ubiquitin-proteasome pathway in systemic lupus erythematosus.","authors":"Chi Chiu Mok","doi":"10.1080/1744666X.2025.2497845","DOIUrl":"10.1080/1744666X.2025.2497845","url":null,"abstract":"Introduction: The ubiquitin-proteasome system (UPS) is the major non-lysosomal mechanism for selective degradation of intracellular proteins that is essential for the regulation of cellular functions and survival. Modulation of the proteasomes and cereblon E3 ligase promotes degradation of polyubiquitin-tagged transcription factors and oncoproteins, leading to depletion of long-lived plasma cells, diminished autoantibody and interferon-α production, reduced T-cell polarization to the proinflammatory phenotypes and increased regulatory T-cell activity that are relevant to the therapy of systemic lupus erythematosus (SLE).Areas covered: Selective immunoproteasome inhibitors and newer generation cereblon modulators have improved safety profiles compared to conventional compounds. This article summarizes the literature regarding the modulation of the UPS in murine and human SLE.Expert opinion: Bortezomib and the selective immunoproteasome inhibitors, ONX-0914 and zetomipzomib, ameliorate renal disease in murine lupus models. While clinically effective in refractory SLE, bortezomib is limited by its toxicities. Zetomipzomib shows promising data in phase Ib/II studies of SLE and lupus nephritis. Thalidomide and lenalidomide are effective in refractory cutaneous lupus but again limited by their off-target effects. A phase II RCT of iberdomide shows favorable results in SLE, especially chronic and subacute cutaneous lesions. These molecules should be further explored in larger clinical trials of renal and cutaneous SLE.","PeriodicalId":12175,"journal":{"name":"Expert Review of Clinical Immunology","volume":" ","pages":"531-542"},"PeriodicalIF":3.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143975161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

NF-κB pathway variants in Iranian patients with inborn errors of immunity. 伊朗先天性免疫缺陷患者NF-κB通路变异

IF 3.9 3区医学

Expert Review of Clinical Immunology Pub Date : 2025-05-01 Epub Date: 2025-05-09 DOI: 10.1080/1744666X.2025.2500608

Nazanin Fathi, Hassan Abolhassani, Fereshte Salami, Tannaz Moeini Shad, Samaneh Delavari, Reza Yazdani, Arash Kalantari, Sareh Sadat Ebrahimi, Nasrin Beniafard, Seyed Alireza Mahdaviani, Nima Rezaei

{"title":"NF-κB pathway variants in Iranian patients with inborn errors of immunity.","authors":"Nazanin Fathi, Hassan Abolhassani, Fereshte Salami, Tannaz Moeini Shad, Samaneh Delavari, Reza Yazdani, Arash Kalantari, Sareh Sadat Ebrahimi, Nasrin Beniafard, Seyed Alireza Mahdaviani, Nima Rezaei","doi":"10.1080/1744666X.2025.2500608","DOIUrl":"10.1080/1744666X.2025.2500608","url":null,"abstract":"Background: Clinical and immunological manifestations associated with genetic alterations are crucial for understanding inborn errors of immunity (IEI). This study aims to characterize the clinical and immunological profiles and provide the molecular features of IEI patients from the Iranian population with IEI who harbor rare variants in the nuclear factor kappa B (NF-κB) pathway.Research design and methods: Peripheral blood mononuclear cells (PBMCs) were used for immunophenotyping of B and T lymphocyte subsets via flow cytometry and for assessing T cell proliferation. Immunoblotting was performed to evaluate the expression levels of NF-κB proteins.Results: This multi-center study enrolled 16 patients with mutations in the NFKB1, NFKB2, IKBKB, and IKBKG genes. NFKB1 and NFKB2 mutations were heterozygous, while IKBKB mutations were homozygous, and the IKBKG mutation was hemizygous. Patients exhibited hypogammaglobulinemia and switched memory B cell abnormalities. Immunoblotting revealed decreased NF-κB1 protein expression in most cases. Similarly, NFKB2 mutations led to lower protein expression in unstimulated PBMCs, with mild to strong reductions after stimulation, though some cases showed no significant changes.Conclusions: This study identifies novel IEI cases associated with NF-κB pathway defects. Further comprehensive evaluation and functional analysis of these mutations are warranted to confirm their impact on disease manifestation.","PeriodicalId":12175,"journal":{"name":"Expert Review of Clinical Immunology","volume":" ","pages":"667-681"},"PeriodicalIF":3.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143990790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Induction of aquaporins by plasma of patients with systemic lupus erythematosus. 系统性红斑狼疮患者血浆对水通道蛋白的诱导作用。

IF 3.9 3区医学

Expert Review of Clinical Immunology Pub Date : 2025-05-01 Epub Date: 2025-05-06 DOI: 10.1080/1744666X.2025.2497841

Hussein Baharlooi, Samaneh Enayati, Nooshin Ahmadzadeh, Elham Madreseh, Seyedeh Tahereh Faezi, Majid Alikhani, Ahmadreza Jamshidi, Mahdi Mahmoudi, Elham Farhadi

{"title":"Induction of aquaporins by plasma of patients with systemic lupus erythematosus.","authors":"Hussein Baharlooi, Samaneh Enayati, Nooshin Ahmadzadeh, Elham Madreseh, Seyedeh Tahereh Faezi, Majid Alikhani, Ahmadreza Jamshidi, Mahdi Mahmoudi, Elham Farhadi","doi":"10.1080/1744666X.2025.2497841","DOIUrl":"10.1080/1744666X.2025.2497841","url":null,"abstract":"Background: Systemic lupus erythematosus is a chronic, multisystemic, inflammatory disease. Aquaporins, a group of transmembrane channels, are known to help prime immune cells and their migration. In this study, a qRT-PCR analysis was performed to identify aquaporins whose expression in SLE patients was associated with the inflammatory profile of B cells.Methods: A stable and healthy line of B cells was cultured and subjected to plasma obtained from SLE patients or healthy individuals for 1 week. Subsequently, gene expression was assessed using real-time PCR.Results: The findings showed that B cells treated with SLE plasma had different expression profiles of inflammatory genes, including TNF-α, IFN-γ, CD40, TNFSF13B, and TNFRSF13C. The study also revealed abnormal expression patterns of aquaporins (AQP3, AQP6, AQP8, and AQP9) in the SLE-treated group. Among the genes, AQP3, AQP6, AQP8, AQP9, and AQP11 were differently correlated with the inflammatory phenotype of B cells. These genes may play a role in the pathogenesis of SLE by affecting B cell proliferation, regulation, inflammation, and cytokine processing.Conclusions: The findings suggest that plasma of SLE patients can induce the inflammatory phenotype of B lymphocytes and the expression of key aquaporin genes, which could impact the development of SLE.","PeriodicalId":12175,"journal":{"name":"Expert Review of Clinical Immunology","volume":" ","pages":"651-658"},"PeriodicalIF":3.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143960436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Re-envisioning clinical trial design in systemic sclerosis. 系统性硬化症临床试验设计的再设想。

IF 3.9 3区医学

Expert Review of Clinical Immunology Pub Date : 2025-05-01 Epub Date: 2025-05-08 DOI: 10.1080/1744666X.2025.2500612

Elizabeth R Volkmann, Donald P Tashkin, Vanessa Smith

{"title":"Re-envisioning clinical trial design in systemic sclerosis.","authors":"Elizabeth R Volkmann, Donald P Tashkin, Vanessa Smith","doi":"10.1080/1744666X.2025.2500612","DOIUrl":"10.1080/1744666X.2025.2500612","url":null,"abstract":"Introduction: Systemic sclerosis (SSc) is a progressive autoimmune rheumatic disease with high morbidity and mortality and few effective treatment options. Increased biopharmaceutical investment in therapeutic development for rare diseases has created new opportunities for drug discovery in SSc. However, despite the increased pipeline activity in SSc, success rates remain dismally low.Areas covered: This review describes the current state of therapeutic development in SSc with an in-depth coverage of cohort enrichment strategies, as well as a discussion of relevant ethical and feasibility concerns. This review also highlights lessons learned from phase 3 trials in SSc published within the last 5 years in PubMed, underscoring the impact of background therapy on SSc disease course during the trial period. Emerging clinical trial formats and endpoints are also explored.Expert opinion: The authors present recommendations to innovate clinical trial design in SSc, which integrate evidence from recent clinical trial and observational cohort studies in SSc. With a focus on the use of external control arms, the application of adaptive trial design and the development of global disease activity measures, the authors outline practical and ethical solutions to design precise and efficient trials in SSc with a higher probability of success.","PeriodicalId":12175,"journal":{"name":"Expert Review of Clinical Immunology","volume":" ","pages":"555-565"},"PeriodicalIF":3.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143988759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0