Induction of aquaporins by plasma of patients with systemic lupus erythematosus.

IF 3.9 3区医学 Q2 IMMUNOLOGY

Expert Review of Clinical Immunology Pub Date : 2025-05-06 DOI:10.1080/1744666X.2025.2497841

Hussein Baharlooi, Samaneh Enayati, Nooshin Ahmadzadeh, Elham Madreseh, Seyedeh Tahereh Faezi, Majid Alikhani, Ahmadreza Jamshidi, Mahdi Mahmoudi, Elham Farhadi

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Abstract

Background: Systemic lupus erythematosus is a chronic, multisystemic, inflammatory disease. Aquaporins, a group of transmembrane channels, are known to help prime immune cells and their migration. In this study, a qRT-PCR analysis was performed to identify aquaporins whose expression in SLE patients was associated with the inflammatory profile of B cells.

Methods: A stable and healthy line of B cells was cultured and subjected to plasma obtained from SLE patients or healthy individuals for 1 week. Subsequently, gene expression was assessed using real-time PCR.

Results: The findings showed that B cells treated with SLE plasma had different expression profiles of inflammatory genes, including TNF-α, IFN-γ, CD40, TNFSF13B, and TNFRSF13C. The study also revealed abnormal expression patterns of aquaporins (AQP3, AQP6, AQP8, and AQP9) in the SLE-treated group. Among the genes, AQP3, AQP6, AQP8, AQP9, and AQP11 were differently correlated with the inflammatory phenotype of B cells. These genes may play a role in the pathogenesis of SLE by affecting B cell proliferation, regulation, inflammation, and cytokine processing.

Conclusions: The findings suggest that plasma of SLE patients can induce the inflammatory phenotype of B lymphocytes and the expression of key aquaporin genes, which could impact the development of SLE.

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系统性红斑狼疮患者血浆对水通道蛋白的诱导作用。

背景：系统性红斑狼疮是一种慢性、多系统、炎症性疾病。水通道蛋白是一组跨膜通道，已知可以帮助原始免疫细胞及其迁移。在本研究中，我们通过qRT-PCR分析来鉴定水通道蛋白，其在SLE患者中的表达与B细胞的炎症谱有关。方法：培养一株稳定健康的B细胞，并将其置于SLE患者或健康人的血浆中1周。随后，使用实时PCR评估基因表达。结果：研究结果显示，SLE血浆处理的B细胞具有不同的炎症基因表达谱，包括TNF-α、IFN-γ、CD40、TNFSF13B和TNFRSF13C。本研究还揭示了水通道蛋白（AQP3、AQP6、AQP8、AQP9）在sle处理组中的异常表达模式。其中，AQP3、AQP6、AQP8、AQP9、AQP11与B细胞炎症表型存在不同程度的相关性。这些基因可能通过影响B细胞增殖、调节、炎症和细胞因子加工在SLE的发病机制中发挥作用。结论：SLE患者血浆可诱导B淋巴细胞炎性表型及关键水通道蛋白基因表达，影响SLE的发展。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Expert Review of Clinical Immunology IMMUNOLOGY-

CiteScore

7.60

自引率

2.30%

发文量

221

审稿时长

6-12 weeks

期刊介绍： Expert Review of Clinical Immunology (ISSN 1744-666X) provides expert analysis and commentary regarding the performance of new therapeutic and diagnostic modalities in clinical immunology. Members of the International Editorial Advisory Panel of Expert Review of Clinical Immunology are the forefront of their area of expertise. This panel works with our dedicated editorial team to identify the most important and topical review themes and the corresponding expert(s) most appropriate to provide commentary and analysis. All articles are subject to rigorous peer-review, and the finished reviews provide an essential contribution to decision-making in clinical immunology. Articles focus on the following key areas: • Therapeutic overviews of specific immunologic disorders highlighting optimal therapy and prospects for new medicines • Performance and benefits of newly approved therapeutic agents • New diagnostic approaches • Screening and patient stratification • Pharmacoeconomic studies • New therapeutic indications for existing therapies • Adverse effects, occurrence and reduction • Prospects for medicines in late-stage trials approaching regulatory approval • Novel treatment strategies • Epidemiological studies • Commentary and comparison of treatment guidelines Topics include infection and immunity, inflammation, host defense mechanisms, congenital and acquired immunodeficiencies, anaphylaxis and allergy, systemic immune diseases, organ-specific inflammatory diseases, transplantation immunology, endocrinology and diabetes, cancer immunology, neuroimmunology and hematological diseases.