Correlation of serum Helios, CD226, tigit, and Foxp3 with tear film osmotic pressure and dry eye disease in patients with rheumatoid arthritis.

IF 3.9 3区医学 Q2 IMMUNOLOGY

Expert Review of Clinical Immunology Pub Date : 2025-05-29 DOI:10.1080/1744666X.2025.2512451

Lijing Huang, Peilin Cheng, Zheli Niu, Liping Zang, Zicong Chen, Chunchun Yang, Wenhua Ma, Wenjia Nie

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引用次数: 0

Abstract

Objective: This study investigated the correlation between serum Helios, CD226, TIGIT, and Foxp3 levels and tear film osmotic pressure in individuals with rheumatoid arthritis (RA) and dry eye disease.

Methods: This case-control study enrolled 100 RA patients with dry eye and 100 healthy controls from May 2021 to June 2022. mRNA expression of target genes was quantified using quantitative real-time PCR (qRT-PCR). Tear film osmolality was measured with a commercial osmometer. Statistical analyses included Pearson correlation and multivariate logistic regression.

Results: Compared with controls, RA patients exhibited significantly higher mRNA levels of Helios, CD226, TIGIT, and Foxp3 (P<0.05), along with elevated mean tear osmolarity (312.5±12.3 vs. 295.4±10.8 milliosmoles per liter (mOsm/L), P<0.05). Notably, 68% of RA patients exceeded the 308 mOsm/L diagnostic threshold versus 22% controls (P<0.05). Helios (r=0.62) and Foxp3 (r=0.58) correlated positively with osmotic pressure, while TIGIT showed a negative correlation (r=-0.49, P<0.05). Logistic regression revealed that elevated levels of Helios (OR=2.1, 95% CI: 1.4-3.2), CD226 (OR=1.8, 95% CI: 1.2-2.7), and Foxp3 (OR=1.9, 95% CI: 1.3-2.8) were associated with increased dry eye risk (P<0.05).

Conclusion: These immune markers demonstrate significant associations with tear film instability in RA, serving as potential biomarkers for ocular comorbidity monitoring.

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类风湿性关节炎患者血清Helios、CD226、tigit和Foxp3与泪膜渗透压和干眼症的相关性

目的：探讨类风湿性关节炎（RA）和干眼病患者血清Helios、CD226、TIGIT和Foxp3水平与泪膜渗透压的相关性。方法：本病例对照研究于2021年5月至2022年6月招募了100名RA干眼患者和100名健康对照者。采用实时荧光定量PCR （qRT-PCR）检测靶基因mRNA表达量。泪膜渗透压用商用渗透计测定。统计分析包括Pearson相关和多元逻辑回归。结果：与对照组相比，RA患者的Helios、CD226、TIGIT和Foxp3 mRNA水平显著升高(p)。结论：这些免疫标志物与RA泪膜不稳定性有显著关联，可作为眼部合并症监测的潜在生物标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Expert Review of Clinical Immunology IMMUNOLOGY-

CiteScore

7.60

自引率

2.30%

发文量

221

审稿时长

6-12 weeks

期刊介绍： Expert Review of Clinical Immunology (ISSN 1744-666X) provides expert analysis and commentary regarding the performance of new therapeutic and diagnostic modalities in clinical immunology. Members of the International Editorial Advisory Panel of Expert Review of Clinical Immunology are the forefront of their area of expertise. This panel works with our dedicated editorial team to identify the most important and topical review themes and the corresponding expert(s) most appropriate to provide commentary and analysis. All articles are subject to rigorous peer-review, and the finished reviews provide an essential contribution to decision-making in clinical immunology. Articles focus on the following key areas: • Therapeutic overviews of specific immunologic disorders highlighting optimal therapy and prospects for new medicines • Performance and benefits of newly approved therapeutic agents • New diagnostic approaches • Screening and patient stratification • Pharmacoeconomic studies • New therapeutic indications for existing therapies • Adverse effects, occurrence and reduction • Prospects for medicines in late-stage trials approaching regulatory approval • Novel treatment strategies • Epidemiological studies • Commentary and comparison of treatment guidelines Topics include infection and immunity, inflammation, host defense mechanisms, congenital and acquired immunodeficiencies, anaphylaxis and allergy, systemic immune diseases, organ-specific inflammatory diseases, transplantation immunology, endocrinology and diabetes, cancer immunology, neuroimmunology and hematological diseases.