European Journal of Soil Biology最新文献

{"title":"Strip-intercropping of eight crop species shows limited within-field variation of soil bacterial communities","authors":"Joliese Teunissen ,&nbsp;Anne Kupczok ,&nbsp;Dirk F. van Apeldoorn ,&nbsp;Stefan Geisen","doi":"10.1016/j.ejsobi.2025.103782","DOIUrl":"10.1016/j.ejsobi.2025.103782","url":null,"abstract":"<div><div>The current crop system needs to become more sustainable to halt the severe environmental impacts associated with intensive monocultures. Alternative agricultural methods, like intercropping, could increase sustainability by better utilizing the biological functions of the plant-soil ecosystem, particularly through plant-associated bacteria. Soil bacteria are key players in supplying many ecosystem functions and thus contribute to plant performance. The effect of intercropping on soil bacteria is a crucial but understudied part of integrating intercropping into the global agricultural system. Here we characterized the effect of intercropping on soil bacterial communities, by comparing intra-versus interspecific crop interactions within one organic strip-intercropping field. We determined the alpha diversity and community composition of soil bacteria across 8 crop species and 16 crop combinations. We found that bacterial alpha diversity was not affected by crop species or crop combination. In contrast, bacterial community composition was influenced by crop species, with certain crops such as parsnip, potato and celeriac shaping their associated bacterial community in both intra- and interspecific crop interactions. Minute differences (&lt;3 %) in soil moisture between crop species determined the strongest patterns observed here. Our findings highlight that crop diversification in the context of strip-intercropping does not always modulate soil bacterial communities under field conditions.</div></div>","PeriodicalId":12057,"journal":{"name":"European Journal of Soil Biology","volume":"127 ","pages":"Article 103782"},"PeriodicalIF":3.3,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145424535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of the seasonal dynamics of grassland soil bacterivores in top- and subsoils","authors":"Verena Groß ,&nbsp;Mathilde Borg Dahl ,&nbsp;Jana Täumer ,&nbsp;Kenneth Dumack ,&nbsp;Haitao Wang ,&nbsp;Michael Bonkowski ,&nbsp;Tim Urich","doi":"10.1016/j.ejsobi.2025.103784","DOIUrl":"10.1016/j.ejsobi.2025.103784","url":null,"abstract":"<div><div>Bacterivorous micro- and macro-organisms are important functional guilds in the soil microbial food web, where their activities influence the community composition. Few studies to date have holistically considered the spatial and seasonal dynamics of such bacterivores in temperate grassland soils; studies of subsoil microbial food webs are particularly scarce. This represents a potential knowledge gap, as environmental conditions (e.g. season and soil depth) may affect prokaryotic and eukaryotic bacterivores differentially. Using a quantitative metatranscriptomic approach, we studied the abundance of SSU rRNA transcripts of bacterivorous bacteria, protists, and nematodes in two German grassland sites from top- and subsoils across seasons. The combined transcript abundance of all bacterivores was significantly reduced with depth. A reduction in the transcript predator-prey ratio was seen for bacterivorous protists, nematodes, and <em>Bdellovibrionota</em>. In contrast, facultative bacterivorous <em>Myxococcota</em> showed a consistently high transcript predator-prey ratio at both depths, suggesting that they dominate the bacterivore functional guild in subsoils. The transcript abundance of all bacterivores and their potential bacterial prey exhibited similar seasonal patterns at both depths. The predator-prey ratio showed bacterivore-specific seasonal and depth-dependent variations. Although this methodology has limitations, as transcript abundance does not directly reflect organism's abundance — our findings suggest a notable role for predatory bacteria as potential main consumers of bacterial biomass in subsoils. In general, increasing soil depth appears to influence the structure of the bacterivorous community, potentially reflected in a shift in the ratio of eukaryotic predators to bacterial prey.</div></div>","PeriodicalId":12057,"journal":{"name":"European Journal of Soil Biology","volume":"127 ","pages":"Article 103784"},"PeriodicalIF":3.3,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145473907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The use of vermicompost from urban organic waste leads to a sustainable increase of earthworm abundance: a three-year study in a crop field converting to organic farming","authors":"Vincent Ducasse ,&nbsp;Vincent Tolon ,&nbsp;Apolline Auclerc ,&nbsp;Line Capowiez ,&nbsp;Joséphine Peigné ,&nbsp;Yvan Capowiez","doi":"10.1016/j.ejsobi.2025.103789","DOIUrl":"10.1016/j.ejsobi.2025.103789","url":null,"abstract":"<div><div>The management of organic matter in sustainable agriculture remains challenging in the 21st century. For farms located close to large cities, the organic fraction of municipal solid waste (OFMSW) is a potential resource to improve soil biological quality. However, this fraction can be processed in very different ways: compost, digestate, or vermicompost. We carried out a three-year trial to compare the effects on earthworm communities of two successive applications of such products at two doses (except for digestate) in a field crop recently converted to organic farming and no tillage. The abandonment of tillage led to a marked increase in the soil bulk density (from 1.25 to 1.45 g cm⁻³) and an associated and continuous decrease in the number of juvenile earthworms in all plots. For abundance of adult earthworms, compost had no effect, whereas digestate led to a large and rapid increase immediately after each application. By contrast, vermicompost steadily increased adult earthworm abundance (+33 to + 90 % compared to the control, depending on the sampling dates). Similar results were observed for earthworm biomass. In this trial, highly dominated by endogeics (80 % of the abundance), no difference between treatments was observed for the temporal variation of each species. However, <em>Aporrectodea caliginosa</em> abundance greatly increased after the first application, whereas <em>Aporrectodea icterica</em> only increased after the second application. The impact of vermicompost on earthworm communities has never been studied under field conditions so far, but this product seems promising for farmers who may seek to benefit from the services provided by earthworms.</div></div>","PeriodicalId":12057,"journal":{"name":"European Journal of Soil Biology","volume":"127 ","pages":"Article 103789"},"PeriodicalIF":3.3,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145620626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Leaf litter quantity shapes oribatid mite assemblages five years following forestry interventions in a European deciduous forest","authors":"Veronika Gergócs-Winkler ,&nbsp;Norbert Flórián ,&nbsp;Bence Kovács ,&nbsp;Réka Aszalós ,&nbsp;András Bidló ,&nbsp;Péter Ódor","doi":"10.1016/j.ejsobi.2025.103790","DOIUrl":"10.1016/j.ejsobi.2025.103790","url":null,"abstract":"<div><div>Continuous cover forestry is widely regarded as a more ecologically sustainable alternative to traditional rotation-based systems in Europe. However, the short- and long-term impacts of different forestry interventions on soil biota remain poorly understood. We investigated the effects of four forest management treatments on soil-dwelling oribatid mites in a European oak–hornbeam forest, five years post-intervention. Assemblages in clear-cut, gap-cut, preparation-cut, and retention tree group plots were compared with controls. Soil fauna were sampled seasonally; climatic conditions were continuously monitored, and vegetation and soil properties measured annually. Mites were identified to species level, and both taxonomic and trait-based community metrics were analysed to assess ecological responses to the treatments. Microclimatic and soil variables did not differ significantly among treatments, but leaf litter quantity was highest in the control, preparation-cutting, and retention tree group plots. Oribatid mite density and species richness were lowest in clear-cutting and gap-cutting plots, highest in the control, and intermediate in the preparation-cutting and retention tree group plots. The most abundant species, mainly from the family Oppiidae, had reduced densities in the more intensively disturbed treatments, contributing to higher evenness in those plots. Less disturbed habitats were dominated by omnivorous, predatory, and scavenger species, with higher proportions of parthenogenetic individuals. In contrast, sexual and predominantly detritivorous species were more prevalent in the clear-cutting and gap-cutting plots. Furthermore, seasonal variation in species composition was more pronounced in these disturbed plots, whereas species composition remained more stable and homogeneous in the control plots. In conclusion, higher amount of leaf litter in less disturbed plots supported the dominance of parthenogenetic and omnivorous species. Oribatid mites proved to be sensitive indicators of long-term ecological effects, highlighting the lasting impact of forestry interventions in oak–hornbeam forests, even five years after disturbance.</div></div>","PeriodicalId":12057,"journal":{"name":"European Journal of Soil Biology","volume":"127 ","pages":"Article 103790"},"PeriodicalIF":3.3,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145620625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The temporal dynamics of soil properties and microbial community structure under capsicum monoculture","authors":"Wangwang Xu ,&nbsp;Zijian Zhao ,&nbsp;JingXia Gao ,&nbsp;Qianqian Ma ,&nbsp;Fengbao Zhang ,&nbsp;Hongbing Li ,&nbsp;Hua Xie","doi":"10.1016/j.ejsobi.2025.103780","DOIUrl":"10.1016/j.ejsobi.2025.103780","url":null,"abstract":"<div><div>The adverse effects of long-term monoculture increasingly threaten the sustainable development of the capsicum industry, leading to soil degradation and yield decline. Although monoculture systems exhibit favorable economic benefits in the early years, these advantages gradually diminish and transform into negative impacts with prolonged cultivation. However, during this transition, the changes in soil physicochemical properties and microbial communities, as well as their potential coupling mechanisms, remain unclear. In this study, soils from capsicum monocultures of 1, 2, 7, and 11 years (Y1, Y2, Y7, and Y11) were used to examine the effects of monoculture duration on soil physicochemical properties and microbial communities. Results showed that with increasing years of monoculture, soil pH in Y2, Y7, and Y11 decreased by 0.03–0.33 units compared with Y1, whereas electrical conductivity (EC), available phosphorus (AP), available potassium (AK), and saturated hydraulic conductivity (SHC) increased by 48.49–87.48 %, 163.24–342.54 %, 43.25–71.57 %, and 80.0–240.0 %, respectively. In contrast, total nitrogen (TN) and soil organic matter (SOM) increased by 24.50 % and 44.44 %, respectively, in short-term monoculture (Y2) compared with Y1, but both gradually declined with increasing monoculture duration. Nitrate nitrogen (NO₃⁻-N) and soil aggregate stability (WAS) remained relatively stable in the short term but declined markedly in the long term, with reductions of 69.30 % and 55.92 % in Y11 compared with Y1. In terms of microbial communities, short-term monoculture increased copiotrophic taxa (e.g., <em>Proteobacteria</em>, <em>Mortierellomycota</em>), whereas long-term monoculture reduced oligotrophic taxa (e.g., <em>Acidobacteriota</em>, <em>Rokubacteria</em>) and key nitrogen-cycling bacteria (e.g., <em>Nitrospira</em>). Simultaneously, long-term monoculture decreased the average degree, network density, and proportion of positive correlations in bacterial and fungal networks, thereby reducing community structural stability. Structural equation modeling (SEM) further indicated that pH, WAS, and NO₃⁻-N were the key factors influencing bacterial communities. These findings reveal that while short-term monoculture promotes nutrient release, long-term monoculture leads to nutrient imbalance, reduced aggregate stability, and disrupted microbial community structures, ultimately weakening carbon and nitrogen cycling functions and severely constraining the sustainable development of the capsicum industry.</div></div>","PeriodicalId":12057,"journal":{"name":"European Journal of Soil Biology","volume":"127 ","pages":"Article 103780"},"PeriodicalIF":3.3,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145424534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Responses of microarthropods and nematodes to long-term warming in alpine meadows are precipitation-dependent effects","authors":"Yao Zhu ,&nbsp;Sisi Tang ,&nbsp;Wei Xue ,&nbsp;Jinhao Ma ,&nbsp;Jiafa Luo ,&nbsp;Lei Hu ,&nbsp;Xiao Ren ,&nbsp;Yuying Wang ,&nbsp;Pengfei Wu","doi":"10.1016/j.ejsobi.2025.103776","DOIUrl":"10.1016/j.ejsobi.2025.103776","url":null,"abstract":"<div><div>Global warming significantly impacts soil fauna in terrestrial ecosystems, yet the differential responses of various groups, such as surface-dwelling microarthropods, soil-dwelling microarthropods, and soil nematodes, remain poorly understood. To address this, a long-term warming experiment using open-top chambers (OTCs) was conducted in alpine meadows of the Qinghai-Tibetan Plateau. Ten years after OTC installation, surface-dwelling, soil-dwelling microarthropods and soil nematodes, plant communities, and soil properties were investigated in September in the normal precipitation year 2019 and in the wet year 2020. The results were that: (1) shifts in taxonomic composition were more pronounced in surface-dwelling microarthropods and soil nematodes than in soil-dwelling microarthropods; (2) warming increased nematode taxonomic richness and Shannon diversity but reduced nematode abundance and surface-dwelling arthropod richness in the normal year, while only nematode abundance increased in the wet year; (3) warming altered nematode trophic structure by decreasing the relative abundance of bacterivores and increasing that of plant-parasites; (4) soil moisture and temperature were the primary drivers of soil faunal community changes, with ammonium nitrogen also being an important factor for soil nematodes. Our results demonstrate that the soil nematodes exhibited the strongest response to warming, followed by surface-dwelling and soil-dwelling microarthropods, and the warming effects on soil fauna abundance and diversity were dependent on precipitation. These taxon-specific sensitivities highlight their utility as bioindicators for monitoring alpine ecosystems.</div></div>","PeriodicalId":12057,"journal":{"name":"European Journal of Soil Biology","volume":"127 ","pages":"Article 103776"},"PeriodicalIF":3.3,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145333460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Low mycovirus presence in soil saprotrophic fungi in Dutch sandy soils","authors":"Ruiqi Wang ,&nbsp;Hazal Kandemir ,&nbsp;Jing Zhang ,&nbsp;T. Martijn Bezemer ,&nbsp;Peter M. van Bodegom ,&nbsp;S. Emilia Hannula","doi":"10.1016/j.ejsobi.2025.103787","DOIUrl":"10.1016/j.ejsobi.2025.103787","url":null,"abstract":"<div><div>Saprotrophic fungi play a fundamental role in soil ecosystems as primary decomposers, driving nutrient and carbon turnover on a global scale. Mycoviruses are considered widespread and can affect saprotrophic fungi by altering their properties, such as growth rate, stress resistance, and metabolite production. To advance the understanding of the prevalence and diversity of mycoviruses of soil saprotrophic fungi, we conducted parallel mycovirus screening in 28 saprotrophic fungi representing major groups of soil fungi and concomitantly explored soil metatranscriptomic data. <em>De novo</em> assembly of RNA-sequencing data uncovered two viruses from two fungal strains. One of these viruses, a ssRNA virus from <em>Mucor</em> sp., appears to represent a putative novel species. Analysis of eukaryotic RNA viruses in soil metatranscriptomes indicated that even though fungal and oomycete hosts made up 39.6 % of the classified eukaryotic viruses, there was no saprotrophic host assignment. Both methods showed that there were few mycoviruses in the Dutch sandy soils with different land uses. This led to the conclusion that mycoviruses affecting saprotrophic fungi may either be rare in these types of soils or more difficult to detect compared to other fungal groups. These results offer new insights into the ecological dynamics and viral associations of soil saprotrophic fungi, highlighting the need for broader sampling and improved approaches to assess mycovirus diversity and its potential ecological significance.</div></div>","PeriodicalId":12057,"journal":{"name":"European Journal of Soil Biology","volume":"127 ","pages":"Article 103787"},"PeriodicalIF":3.3,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145575906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heterogeneous salinity and nitrogen in the root-zone influences soil water status, rhizosphere bacteria distributions and promotes tomato growth","authors":"Yuexiong Wang ,&nbsp;Zhenchang Wang ,&nbsp;Jinjing Liu ,&nbsp;Rangjian Qiu ,&nbsp;Cheng Hong ,&nbsp;Minghao Tian ,&nbsp;Kexin Chen ,&nbsp;Xiaoman Qiang","doi":"10.1016/j.ejsobi.2025.103781","DOIUrl":"10.1016/j.ejsobi.2025.103781","url":null,"abstract":"<div><div>Soil texture heterogeneity together with agronomic practices like drip irrigation, furrow-bed seeding, and straw mulching often introduces abrupt changes in soil physical properties, causing uneven soil salinity (Sa) and nitrogen (N) distribution in the root-zone. While rhizosphere bacterial and plant responses to Sa or N heterogeneity have been widely investigated, N supply modes may alter root responses to heterogeneous Sa stress and simultaneously influence bacterial communities. To elucidate the coupled impacts of heterogeneous Sa and N on plant growth and rhizosphere bacteria as well as their relationships, two Sa distribution patterns (Sa1: 1/5 g kg⁻¹ NaCl on the low-/high-salinity sides; Sa2: 3/3 g kg⁻¹ NaCl) and three N supply modes (N1: 270/0 mg kg⁻¹ N; N2: 0/270 mg kg⁻¹ N; N3: 135/135 mg kg⁻¹ N) were implemented. Herein, 16S rRNA gene amplicon sequencing and co-occurrence network analysis revealed bacterial community characteristics and network topological features under different treatments. We found that compared to uniform Sa distribution, uneven distribution of Sa significantly increased root biomass and surface area on the low-salinity side, thereby facilitating compensatory uptake of water and nutrients and ultimately increasing tomato total biomass and N content. Besides, N supply modes altered plant root responses to Sa heterogeneity, with N2 and N3 tomatoes exhibiting greater total N content and biomass in plants than N1 under the Sa1 distribution. Compared to the Sa2N3 treatment, the Sa1N2 treatment increased the Chao1 index and enriched beneficial core rhizobacteria such as <em>Acidobacteriota</em>, enhancing potential cooperation among rare taxa. The above shifts were primarily driven by heterogeneous Sa, which altered soil water content, soil NO₃-N content, EC_1:5, and root distribution, thereby restructuring bacterial community composition and co-occurrence network patterns. The SEM model further indicated that variations in bacterial diversity (Chao1 index) reshaped functional profiles, thereby regulating N availability. Additionally, the Chao1 index exhibited significant positive relationships with tomato total biomass, particularly Chao1 index on the low-salinity side. This study highlights the importance of bacterial diversity of rare taxa as predictors for reflecting the coupling effect of heterogeneous salinity and nitrogen on plant growth, thereby deepening our understanding of the contribution of bacteria in plant responses to salinity-nitrogen heterogeneity.</div></div>","PeriodicalId":12057,"journal":{"name":"European Journal of Soil Biology","volume":"127 ","pages":"Article 103781"},"PeriodicalIF":3.3,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145424536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial necromass carbon mediates nonlinear soil carbon response to nitrogen application in the greenhouse vegetable production system","authors":"Zhou Jia ,&nbsp;Lifeng Zhang ,&nbsp;Chengzhang Wang ,&nbsp;Longgang Jiang ,&nbsp;Erxiong Zhu ,&nbsp;Meng Li ,&nbsp;Ruonan Li ,&nbsp;Li Guo ,&nbsp;Yihong Li ,&nbsp;Liying Wang ,&nbsp;Jianshuo Shi","doi":"10.1016/j.ejsobi.2025.103788","DOIUrl":"10.1016/j.ejsobi.2025.103788","url":null,"abstract":"<div><div>Soil organic carbon (SOC) constitutes the largest terrestrial C reservoir, playing a pivotal role in global C cycling. Nitrogen (N) fertilization has been widely recognized as a major driver of SOC transformation and accumulation. However, the specific mechanistic pathways by which long-term differential N application rates modulate microbially derived SOC components and thereby influence SOC persistence in greenhouse vegetable production (GVP) systems remain to be elucidated. We implemented a 12-year field trial assessing five chemical N application rates (cucumber/tomato season: 0/0 (N0), 200/200 (N200), 300/225 (N300), 600/450 (N600), and 900/675 (N900) kg N ha⁻¹ yr⁻¹) in an intensive GVP system. This study integrates measurements of microbial necromass carbon (MNC), kinetics of C- and N-acquiring hydrolases, soil chemical properties and phospholipid fatty acids (PLFAs) to unravel the impacts of N application rates on SOC dynamics and persistence. Our results demonstrate that MNC constitutes 54.5–77.0 % of SOC across all N addition treatments, with fungal necromass C (FNC) averaging 4.40 times higher than bacterial necromass C (BNC). Interestingly, our findings revealed that the MNC/SOC was nonlinearly correlated with elevated N addition rates. Specifically, the BNC/SOC peaked under the N600 treatment, whereas the FNC/SOC was maximized under the N300 treatment and then showed a downward trend. Hierarchical partitioning results indicated that enzymatic kinetics as the predominant regulator of both MNC/SOC and FNC/SOC, whereas soil nutrient attributes exerted stronger control over BNC/SOC. Moreover, the PLS-PM results identified soil properties (e.g., Total N, C:N, NO₃⁻-N, dissolved organic C, pH, and electrical conductivity) as the primary positive regulators of BNC/SOC. However, FNC/SOC and MNC/SOC were predominantly driven by the kinetics of C- (α-glucosidase, β-glucosidase, β-xylosidase) and N-acquiring (N-acetyl-glucosaminidase) enzymes. Therefore, these drivers exhibited diametrically opposing effects on BNC/SOC relative to FNC/SOC (MNC/SOC). Our findings establish that optimized N management is critical for sustaining SOC sequestration in GVP systems, providing a mechanistic basis for linking N application to microbial-mediated C stabilization in the intensive agriculture systems.</div></div>","PeriodicalId":12057,"journal":{"name":"European Journal of Soil Biology","volume":"127 ","pages":"Article 103788"},"PeriodicalIF":3.3,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145575912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Soil pH and organic phosphorus co-shape the diversity and assembly processes of arbuscular mycorrhizal fungal community in subtropical broadleaved forests","authors":"Rui Xu ,&nbsp;Zhucheng Yu ,&nbsp;Yang Liu ,&nbsp;Yidan Liu ,&nbsp;Xingcong Ma ,&nbsp;Xiaomin Ma ,&nbsp;Wenhao Jin ,&nbsp;Yongfu Li ,&nbsp;Junhui Chen ,&nbsp;Hua Qin","doi":"10.1016/j.ejsobi.2025.103785","DOIUrl":"10.1016/j.ejsobi.2025.103785","url":null,"abstract":"<div><div>Arbuscular mycorrhizal (AM) fungal represent a vital pathway for plant nutrient acquisition in soils, especially in nutrient-limited subtropical broadleaved forests where AM fungi extensively colonize roots. However, critical knowledge gaps persist regarding how soil phosphorus and nitrogen gradients (including organic and inorganic forms) influence the structure and assembly mechanisms of AM fungal communities in subtropical broadleaved forests. We conducted field sampling of <em>Schima superba</em> rhizosphere soils (typically AM tree species), analyzing the concentrations of key soil nutrient forms (e.g., available phosphorus, organic phosphorus, ammonium, nitrate) and the AM fungal community structure via high-throughput sequencing of the 18S rRNA gene. Correlation revealed that AM fungal biomarkers (NLFA 16:1ω5) increased with soil pH. AM fungal richness exhibited a significant quadratic relationship with soil organic phosphorus (OP) concentrations and was negatively correlated with the available phosphorus (AP) to OP ratio, suggesting that a higher proportion of readily available phosphorus suppresses AM fungal diversity. Community structure (indicated by NMDS1) was primarily influenced by total phosphorus (TP) concentrations and pH, while network complexity was significantly associated with AP and NH₄⁺-N. The assembly of AM fungal communities was dominated by deterministic processes, whose influence decreased with increasing soil OP and TP concentrations. Random forest analyses and structure equation modeling (SEM) demonstrated that the structure and assembly of AM fungal communities were jointly shaped by soil pH and P dynamic (especially OP and TP). Overall, these findings elucidate that soil pH and P forms (particularly OP) are pivotal factors governing the structure and assembly processes of AM fungal communities in subtropical broadleaved forests, advancing our understanding of AM fungal community variation in nutrient-poor environments.</div></div>","PeriodicalId":12057,"journal":{"name":"European Journal of Soil Biology","volume":"127 ","pages":"Article 103785"},"PeriodicalIF":3.3,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145473908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}