European Journal of Soil Biology最新文献

{"title":"Interplanting Phoebe bournei modifies soil microbial community characteristics in Cunninghamia lanceolata monocultures","authors":"Dan Zhou ,&nbsp;Chengjian Hong ,&nbsp;Jiahuan Guo ,&nbsp;Chang Pan ,&nbsp;Yazhou Tang ,&nbsp;Jie Yan ,&nbsp;Kaizhi Xie ,&nbsp;Yuanchun Yu","doi":"10.1016/j.ejsobi.2025.103762","DOIUrl":"10.1016/j.ejsobi.2025.103762","url":null,"abstract":"<div><div>The prolonged monoculture of <em>Cunninghamia lanceolata</em> depletes soil fertility, making the introduction of broadleaf trees—<em>Phoebe bournei</em> indispensable for soil restoration—yet its impact on the belowground ecological environment remains inadequately explored. Here, we investigated how uneven-aged interplanting of <em>P. bournei</em> in subtropical <em>C. lanceolata</em> stands alters microbial community characteristics and correlates with major edaphic variables across 0–60 cm soil profile. Interplanting <em>P. bournei</em> improved soil aeration, water conservation, and nutrient availability, driving significant shifts in bacterial and fungal β-diversity and partial but significant changes in community composition. It also enhanced ecological drift and reduced dispersal limitation, strengthening homogeneous selection in bacterial communities (0–40 cm) while promoting drift in fungal communities (0–60 cm). These structural and assembly changes suggest potential functional alterations in organic-matter degradation and nutrient cycling. Interplanting <em>P. bournei</em> led to marked improvements in soil microbial ecology, as evidenced by higher abundances of Acidobacteria and Ascomycota, indicative of enhanced complex-carbon degradation; elevated bacterial cellulolytic capacity and accelerated organic-matter turnover; promotion of saprotroph–symbiotroph nutrition; and reduced subsoil pathotroph abundance, which may indirectly support <em>C. lanceolata</em> health. Lastly, soil nutrient elements (e.g., soil organic carbon, total phosphorus) and enzyme activities (e.g., Cellobiohydrolase, Sucrase) were identified as key drivers of microbial community structure and functional potential, highlighting their critical roles in shaping soil microbial ecosystems. In summary, uneven-aged interplanting <em>P. bournei</em> in <em>C. lanceolata</em> plantations optimized soil ecosystem functions, offering a sustainable strategy to enhance forest productivity and improve soil health.</div></div>","PeriodicalId":12057,"journal":{"name":"European Journal of Soil Biology","volume":"126 ","pages":"Article 103762"},"PeriodicalIF":3.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144920215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes in carbon and nitrogen stable isotopic ratios with decomposition of plant residue","authors":"Xiaodong Chen ,&nbsp;Ryoko Senda ,&nbsp;Yuki Mori ,&nbsp;Syuntaro Hiradate","doi":"10.1016/j.ejsobi.2025.103755","DOIUrl":"10.1016/j.ejsobi.2025.103755","url":null,"abstract":"<div><div>The transformation of plant residue into soil organic matter (SOM) plays a crucial role in maintaining the function of ecosystems. To elucidate the formation processes of SOM from plant residue, we examined changes in carbon (C) and nitrogen (N) contents, along with δ¹³C and δ¹⁵N values, during the decomposition of <em>Artemisia princeps</em> residue in an incubation experiment using 100 % quartz sand as an artificial soil. The results indicated that plant residue decomposition occurred in two distinct stages: (1) a rapid initial loss of C and N (43–54 % and 36–47 %, respectively) within the early 90 days, followed by (2) a slower loss (4–14 % and 7–15 %, respectively) over the remaining 90–360 days. In the early stage, δ¹³C and δ¹⁵N values increased similarly to trophic fractionation, with a δ¹⁵N/δ¹³C ratio of 1.54. In the latter stage, this ratio increased significantly to 12.8, probably due to increased C resistance to decomposition, while continuous N metabolism. By integrating data from both the literature and our study, we concluded that C and N in plant residue undergo one to two times the metabolic turnover relative to trophic turnover to form stable SOM. These findings are essential for understanding the preservation of plant residue, particularly labile compounds, in soils. This preservation would occur through microbial transformation. Our study offers a theoretical framework for understanding the SOM dynamics based on δ¹³C and δ¹⁵N values, emphasizing the utility of stable C and N isotope analyses in elucidating SOM transformation.</div></div>","PeriodicalId":12057,"journal":{"name":"European Journal of Soil Biology","volume":"126 ","pages":"Article 103755"},"PeriodicalIF":3.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144581421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of the soil springtail Folsomia candida on the composition, function, and microbial network of root-associated microbes of host plant Sedum plumbizincicola","authors":"Shaobing Li ,&nbsp;Liming Pu ,&nbsp;Zinan Wang ,&nbsp;Siyao Liu ,&nbsp;Fang Liang ,&nbsp;Yuping Ma ,&nbsp;Mingyun Jia ,&nbsp;Zhu Li ,&nbsp;Xin Ke ,&nbsp;Longhua Wu","doi":"10.1016/j.ejsobi.2025.103748","DOIUrl":"10.1016/j.ejsobi.2025.103748","url":null,"abstract":"<div><div>Plant root-associated microorganisms play a major role in promoting plant growth and suppressing pathogenic bacteria. Soil animals in complex soil systems, and especially springtails, interact closely with plants and microorganisms by preying on microorganisms and feeding on roots. However, currently there is a lack of knowledge about how soil springtails modify microbes associated with plant roots. Here the bacterial and fungal communities in the rhizosphere and endosphere of <em>Sedum plumbizincicola</em> in the presence of a soil springtail were investigated. 16S rRNA and ITS gene sequencing were used together with assessments of microbial biomass and carbon source utilization in the rhizosphere. The presence of the springtail did not impact the overall diversity of the microbial community or its assembly processes but it did alter the abundance of specific microorganisms. The springtail influenced the relative abundance of the genus <em>Pseudomonas</em> and the class Nitrospira in the rhizosphere. Moreover, the springtail increased the biomass and carbon source utilization of rhizosphere microbes and influenced the abundance of nitrogen cycle genes. Co-occurrence network analysis revealed an increase in the average degree and total number of nodes and edges within the microbial network in the presence of the springtail, indicating heightened microbial interactions and a more stable network. The results indicate that the springtail regulated the ecological functions of plant root-associated microorganisms and highlight for the first time the role of soil springtails in regulating the microbial community and functions associated with plant roots.</div></div>","PeriodicalId":12057,"journal":{"name":"European Journal of Soil Biology","volume":"126 ","pages":"Article 103748"},"PeriodicalIF":3.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144366465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From fire to soil: the role of prescribed burns in land snail communities for improve conservation","authors":"Meritxell Soler Brugués ,&nbsp;Vicenç Bros ,&nbsp;Santi Ramos ,&nbsp;Roger Puig-Gironès","doi":"10.1016/j.ejsobi.2025.103749","DOIUrl":"10.1016/j.ejsobi.2025.103749","url":null,"abstract":"<div><div>As climate change increases fire intensity and frequency, prescribed burns have become a key tool in ecosystem management. This study examines the effects of prescribed burning, burn frequency, and habitat heterogeneity on soil-dwelling snail communities in Mediterranean ecosystems, aiming to improve fire management to conserve biodiversity while reducing fire risks. Conducted in the Massís del Montgrí (NE Iberian Peninsula), 40 subplots with varying burn histories were sampled. Gastropods were surveyed through active searches for larger snails and soil samples for smaller ones. Vegetation cover, leaf litter, and organic matter content were also measured. Prescribed burns generally reduced snail abundance and richness, with unburnt control areas hosting more snails. Small species were more common in unburnt plots with higher tree cover and organic matter content, while larger species generally thrived in frequently burned areas with higher herbaceous cover. Burn frequency and vegetation cover were key factors shaping snail communities, highlighting the role of fire-driven microhabitat changes – particularly through their effects on organic matter availability – in shifting soil-dwelling snail assemblages. By identifying species-specific responses to fire, this study contributes to our understanding of how fire shapes soil biodiversity and offer insights for prescribed burn regime management. We advocate for context-dependent Integrated Fire Management strategies that balance fire recurrence and intensity with habitat recovery. Adaptive management approaches and ongoing interdisciplinary monitoring will be essential to optimizing fire practices for biodiversity conservation.</div></div>","PeriodicalId":12057,"journal":{"name":"European Journal of Soil Biology","volume":"126 ","pages":"Article 103749"},"PeriodicalIF":3.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144321759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ant-isopod interactions: spanning predation, facilitation, and myrmecophily","authors":"Thomas Parmentier ,&nbsp;Stefano Taiti ,&nbsp;Pallieter De Smedt","doi":"10.1016/j.ejsobi.2025.103753","DOIUrl":"10.1016/j.ejsobi.2025.103753","url":null,"abstract":"<div><div>Ants and terrestrial isopods are two key groups within soil ecosystems both in terms of their biomass and the vital functions they contribute to. Despite the ecological importance of both groups and their frequent interactions, the dynamics between ants and terrestrial isopods remain poorly understood. We provide a systematic overview and discuss the diverse spectrum of ant-isopod interactions ranging from predation, through facilitation, to myrmecophily.</div><div>A limited number of ant species, restricted to the genus <em>Leptogenys</em>, specialize in preying on isopods, while a very broad group of ants opportunistically include them in their diet. To evade ant predation, terrestrial isopods employ defensive strategies such as avoidance, conglobation or secretion of repellent substances. Apart from the direct negative impacts of predation, ants impose several non-lethal effects on terrestrial isopods, influencing their spatial distribution, behaviour, and fitness. Invasive ants may, in contrast, indirectly benefit isopods by feeding on their predators or competitors. A diverse group of isopods are not deterred by ants and reside in their nests. These myrmecophilous isopods vary from facultative associates, which cohabit with ants only occasionally and are represented by many records, to obligate guests that live permanently within ant nests. These obligate guests thrive in ant fortresses by specific behavioural, chemical and morphological adaptations. Their effect on the host can range from negative to neutral, or potentially even beneficial. Though modest in number, with 23 species across 10 genera, the diversity of obligate myrmecophilous isopods showcases a fascinating pattern of independent evolution in soil ecosystems.</div></div>","PeriodicalId":12057,"journal":{"name":"European Journal of Soil Biology","volume":"126 ","pages":"Article 103753"},"PeriodicalIF":3.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144633561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering the microbial mechanisms underlying glucose induced soil priming effects under low and high nutrient levels","authors":"Jiaqi Wang ,&nbsp;Xichang Zhang ,&nbsp;Yingyi Fu ,&nbsp;Lukas Van Zwieten ,&nbsp;Han Sun ,&nbsp;Georg Guggenberger ,&nbsp;Lingfei Hu ,&nbsp;Yu Luo ,&nbsp;Tida Ge ,&nbsp;Yakov Kuzyakov","doi":"10.1016/j.ejsobi.2025.103757","DOIUrl":"10.1016/j.ejsobi.2025.103757","url":null,"abstract":"<div><div>Soil priming effect, which refers to the impact of labile carbon inputs on the decomposition of soil organic matter, plays a significant role in carbon storage. Investigating substrate induced soil priming effects and the involved microbial mechanisms, particularly under nutrients gradient, is central to the understanding of carbon processes and potential accrual in agriculture soil. Thus, a 7 days laboratory incubation was conducted to assess ¹³C labeled glucose induced priming effects in soil receiving the high (TH) and low nutrient (TL) addition. Also, DNA-SIP coupled with metagenomic were adopted to identify the core microbial groups and functional guild responsible for soil priming effects. Here, we found that i) soil priming effects were significantly larger in TL treatment than in the TH treatment, and ii) the larger priming in TL was likely driven by N-mining processes dominated by K-strategy microbes, whereas less priming in TH might be explained by co-metabolism led by r-strategy microbes. Additionally, functional changes of microbial community were revealed by Shotgun sequencing. Both KEGG, EggNOG and CAZymes showed the relative abundance of the functional genes (e.g., GH13_10 and GH77) encoding cellulase enzymes involved in soil organic carbon decomposition were more abundant in TL compared to TH, suggesting higher priming effects in TL was mainly due to the nutrient constraints on microbial demands. This study revealed the main microbial groups and their functions in glucose induced soil priming effects under low and high nutrient levels.</div></div>","PeriodicalId":12057,"journal":{"name":"European Journal of Soil Biology","volume":"126 ","pages":"Article 103757"},"PeriodicalIF":3.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144655953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diazotrophic communities shift with organic fertilizer substitution and growth stages in tomato field soil","authors":"Chunmiao Lu ,&nbsp;Jiahui Luo ,&nbsp;Tianlong Sheng ,&nbsp;Yuebin Xie ,&nbsp;Yuchen Xian ,&nbsp;Yanqiong Jiang ,&nbsp;Xiangzhen Li ,&nbsp;Minjie Yao","doi":"10.1016/j.ejsobi.2025.103746","DOIUrl":"10.1016/j.ejsobi.2025.103746","url":null,"abstract":"<div><div>Applying organic fertilizers can improve soil quality, promote the growth of nitrogen-fixing bacteria, and reduce dependence on chemical fertilizers. Here we investigated the effects of different organic fertilization treatments (organic fertilizer substituting 20 %, 40 % or 60 % of chemical nitrogen fertilizer and increased organic fertilizers at the rate of 45, 90 and 135 t ha⁻¹) on soil diazotrophic communities at different growth stages (seedling, flowering, fruiting and maturity) of tomato. The results indicated that both organic fertilizer treatments and growth stages significantly affected the diversity and compositions of soil diazotrophic community, and organic fertilizer had a greater effect than growth stages. Compared to chemical fertilizer, the organic substitution overall increased the relative abundances of <em>Bradyrhizobium</em>, <em>Skermanella</em>, <em>Paenibacillus</em> and <em>Azospirillum</em>. Increased organic fertilizers raised the relative abundances of <em>Methylocaldum</em> and <em>Hyphomicrobium</em>. Organic substitution treatments increased the network complexity and microbial interactions of diazotrophic communities, but increased organic fertilizers reduced the alpha diversity and network complexity. Organic fertilizer affected the diazotrophic community structure and key taxa mainly through altering soil available nutrients and pH. The key diazotrophic genera varied at different growth stages. <em>Azospirilum</em> and <em>Skermanella</em> played an important role in diazotrophic community assembly in the flowering stage, additionally, <em>Sinorhizobium</em>, <em>Paenibacillus</em>, and <em>Zoogloea</em> were important in the fruiting stage. This study provided a deep understanding of the roles of organic fertilizer in regulating soil diazotrophic communities in tomato field.</div></div>","PeriodicalId":12057,"journal":{"name":"European Journal of Soil Biology","volume":"126 ","pages":"Article 103746"},"PeriodicalIF":3.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144290447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Crop rotation and organic fertilizer maintains diversity and activity of cbbL-carrying CO2-fixing bacteria in reclaimed coal mining soils","authors":"Shuning Bai ,&nbsp;Meihua Fan ,&nbsp;Min Wu ,&nbsp;Xiaolong Sui ,&nbsp;Yibo Song ,&nbsp;Yunlong Jiang ,&nbsp;Huisheng Meng ,&nbsp;Yulin Liu ,&nbsp;Xiangying Wang ,&nbsp;Xianjun Hao ,&nbsp;Yi Li ,&nbsp;Jianping Hong ,&nbsp;Jie Zhang","doi":"10.1016/j.ejsobi.2025.103759","DOIUrl":"10.1016/j.ejsobi.2025.103759","url":null,"abstract":"<div><div>CO₂-fixing bacteria are an important factor in restoring soil health in coal mining areas. The impact of crop rotation and fertilization on CO₂-fixing bacteria in reclaimed mining soils remains unclear. To narrow this knowledge gap, in this study, maize (<em>Zea mays</em> L.) monoculture (M) and maize-soybean (<em>Glycine max</em>) rotation (R) cropping systems were set up in a coal-mining reclamation area with four fertilization treatments in each, namely, CK (without fertilization), inorganic fertilizer (F), organic fertilizer (O), and combined organic and inorganic fertilizer (OF). The abundance, diversity, community composition and RubisCO activity of CO₂-fixing bacteria in topsoil under those treatments were investigated respectively using quantitative PCR, high-throughput sequencing based on the <em>cbbL</em> gene [that encodes ribulose-1,5-biphosphate carboxylase/oxygenase (RubisCO)] and enzyme-linked immunosorbent assay (ELISA). The results showed that R_O significantly increased easily oxidized organic carbon (EOC), total nitrogen (TN), and available nitrogen (AN) (<em>P</em> &lt; 0.05). It also significantly increased the biomass of CO₂-fixing bacteria (<em>P</em> &lt; 0.05) and altered the CO₂-fixing bacterial community. The CO₂-fixing bacteria in R_OF, R_O and M_O exhibited comparable community structures and harbored a greater co-occurrence network complexity than other treatments. Several CO₂-fixing bacteria associated with nitrogen cycling, such as <em>Devosia</em>, <em>Nitrobacter</em>, Hyphomicrobiales and <em>Nitrosospira</em>, were significantly enriched under the maize-soybean rotation system (<em>P</em> &lt; 0.05). This study implied that crop rotation and organic fertilizer application could synergistically foster soil quality restoration in coal mining area by elevating soil nutrients and maintaining biomass, diversity and community structure of <em>cbbL</em>-carrying CO₂-fixing bacteria, establishing a theoretical foundation for optimizing carbon sequestration strategies in post-mining ecological rehabilitation.</div></div>","PeriodicalId":12057,"journal":{"name":"European Journal of Soil Biology","volume":"126 ","pages":"Article 103759"},"PeriodicalIF":3.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144739405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Substitution of organic fertilizer did not change the dominant role of biochar on bacterial community stability but the soil carbon fractions","authors":"Husen Qiu ,&nbsp;Jieyun Liu ,&nbsp;Tida Ge","doi":"10.1016/j.ejsobi.2025.103744","DOIUrl":"10.1016/j.ejsobi.2025.103744","url":null,"abstract":"<div><div>Biochar and organic fertilizers are used to improve soil carbon retention. However, the interactive roles of biochar and organic fertilizer in stabilizing soil organic carbon (SOC) at different horizons remain unclear. A field experiment with biochar application (0 %, 1 %, and 2 % of dry soil; Control, LB, and HB, respectively) and organic fertilizer substitution (0 %, 20 %, 40 %, and 60 % of inorganic N; T0, T2, T4, and T6, respectively) (fermented sheep manure replacing urea-N)) was conducted to reveal the mechanisms. The SOC, particulate organic carbon (POC), and mineral-associated organic carbon (MAOC) contents increased with the biochar addition and organic fertilizer substitution. In the topsoil (0–15 cm), values ranged from 17 to 24 g kg⁻¹ (SOC), 2.03–6.6 g kg⁻¹ (POC), and 15–19 g kg⁻¹ (MAOC). In the subsoil (16–30 cm), the ranges were 16–20 g kg⁻¹ (SOC), 1.2–3.4 g kg⁻¹ (POC), and 14–18 g kg⁻¹ (MAOC). HB combined with organic fertilizer substitution promoted SOC, POC, and MAOC more effectively than biochar alone. The β-1,4-glucosidase and cellobiohydrolase (CBH) activities in the topsoil were 1.2–1.6 times higher than those in the subsoil. Increases in biochar reduced the POC/MAOC ratio by limiting CBH activity (P &lt; 0.05). Substituting organic fertilizer increased the POC/MAOC ratio by alleviating the limitation of biochar on bacterial biomass in the soil horizons. Bacterial community composition varied significantly with biochar addition and between soil horizons (P &lt; 0.05). Biochar enhanced the dominant role of heterogeneous selection and increased bacterial network complexity in both the topsoil and subsoil. Based on structural equation modeling, an increase in bacterial negative/positive cohesion significantly improved the proportion of MAOC in the topsoil (P &lt; 0.05). This study provides evidence that biochar (but not organic fertilizer substitution) helped improve C storage by decreasing the CBH activity and bacterial biomass, and enhancing network complexity. Thus, it is essential to consider soil horizons when evaluating the dynamic effects of agricultural practices on SOC pools.</div></div>","PeriodicalId":12057,"journal":{"name":"European Journal of Soil Biology","volume":"126 ","pages":"Article 103744"},"PeriodicalIF":3.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144239503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanisms of furrow-applied biochar in enhancing rhizosphere soil microbiota and metabolites in continuous sesame cropping","authors":"Ruiqing Wang ,&nbsp;Fengjuan Lyu ,&nbsp;Rujie Lyu ,&nbsp;Junhai He ,&nbsp;Lingen Wei","doi":"10.1016/j.ejsobi.2025.103763","DOIUrl":"10.1016/j.ejsobi.2025.103763","url":null,"abstract":"<div><div>Biochar application significantly reduces bacterial wilt and boosts yield in continuous sesame cropping, but high costs limit its widespread use. This study used metagenomic and metabolomic analyses to evaluate three biochar application strategies (surface [BR3, 15,000 kg ha⁻¹], furrow [BR4, 1,875 kg ha⁻¹], and no biochar [BR0]) across four sample types (D0/D1: diseased; H0/H1: healthy; rhizosphere/non-rhizosphere), analyzing their effects on microbial community as well as their functional and metabolic profiles in sesame soil. The results showed that BR4 significantly increased sesame yield by 26.57 % compared to the control (BR0). Biochar treatments (BR3/BR4) enhanced the relative abundance of <em>Actinomycetota</em> while suppressing <em>Pseudomonas</em> in rhizosphere and non-rhizosphere soils of diseased plants. <em>Sphingobium amiense</em> and <em>S. indicum</em> were differentially abundant in BR3H0 and BR4H0, respectively. We observed disruption of fatty acid metabolism in BR3D0, which was linked to growth inhibition in continuously cropped sesame. BR4H0 treatment significantly increased the relative abundance of pyruvate dehydrogenase and the superpathway for de novo biosynthesis of pyrimidine ribonucleotides. The rhizospheres treated with BR4 from healthy plants showed higher levels of C17-sphinganine (+2.79 percentage points) and 6-[3-(dihydroxyphenyl-methyl)-methoxybutyl]-benzodioxole (+0.18 percentage points) compared to diseased plants. Partial Least Squares Discriminant Analysis (PLS-DA) identified Jasmine lactone and Tris(hydroxymethyl)aminomethane as key metabolite discriminators under positive ion mode, as opposed to Taurochenoxycholate and Palmitic acid under negative ion mode. These findings suggest that furrow-applied biochar at a reduced rate (1,875 kg ha⁻¹) can enhance soil microbial structure, function, and metabolite composition, ultimately improving yield in continuous sesame cropping systems.</div></div>","PeriodicalId":12057,"journal":{"name":"European Journal of Soil Biology","volume":"126 ","pages":"Article 103763"},"PeriodicalIF":3.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144908200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}