European Journal of Soil Biology最新文献

{"title":"Fire and clipping drive microbial fixation pathways in soil phosphorus and sulfur cycling in China's key karst region","authors":"Ansa Rebi ,&nbsp;Guan Wang ,&nbsp;Irsa Ejaz ,&nbsp;Trevan Flynn ,&nbsp;Jasper Kanomanyanga ,&nbsp;Tao Yang ,&nbsp;Adnan Mustafa ,&nbsp;Jinxing Zhou","doi":"10.1016/j.ejsobi.2024.103707","DOIUrl":"10.1016/j.ejsobi.2024.103707","url":null,"abstract":"<div><div>Despite growing interest in nutrient cycling genes, the influence of fire and clipping on soil microbes, phosphorus (P) and sulfur (S) cycling genes in Karst landscape remain unclear yet are critical for soil fertility in vegetation restoration landscape. Microorganisms have developed various adaptive mechanisms to improve nutrient availability in the soil in response to various landscape disturbances. In this study, we analyzed soil microbial communities and their role in mediating 90 P and 46 S genes under five fire and clipping management practices including: high-intensity fire (HIF), low-intensity fire (LIF), clipping and fire (CF), clipping (CP), and undisturbed control (CK) in Jianshui research station, Yunan province, China. The results indicated no significant (p &lt; 0.05) differences in the predominant bacterial and fungal genera among the treatments. For bacterial compositions such as Sphingomonas, the relative abundance was highest (0.069 %) in LIF. In contrast, the relative abundance of Micromonospora was lowest (0.012 %) in LIF compared to CK. In the case of the fungal genus, Rhizophagus and Trichophyton were highest (0.187, 0.128 %) in CP and LIF respectively compared to control. Bacterial diversity was highest in CF (4.69) following the CK (4.71) while Fungal diversity was highest in CP (3.33) following the CK. P cycling genes increased in LIF, particularly those related to organic phosphoester hydrolysis and transporters, while the other treatments showed no considerable changes. S cycling genes related to S mineralization and assimilation increased in HIF and LIF, respectively, with CF showing a higher presence of sulfide cycling genes. Network analysis of P and S cycling genes indicated that S interactions formed tighter clusters under fire and clipping treatments, while P interactions had more extensive connectivity among genes. These findings underscore the distinct roles and network behaviours of P and S and provide valuable insights into the microbial mechanisms that regulate P availability and S cycling in Karst soils treated with fire and clipping. This also sheds light on the taxonomy of the microbes involved in informed decision-making in karst landscape management.</div></div>","PeriodicalId":12057,"journal":{"name":"European Journal of Soil Biology","volume":"124 ","pages":"Article 103707"},"PeriodicalIF":3.7,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143152989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Warmer summers have the potential to affect food security by increasing the prevalence and activity of Actinobacteria","authors":"Yuanyuan Bao ,&nbsp;Jan Dolfing ,&nbsp;Zhiying Guo ,&nbsp;Jie Liu ,&nbsp;Xianzhang Pan ,&nbsp;Xiaodan Cui ,&nbsp;Yuanyuan Wang ,&nbsp;Yang Jin ,&nbsp;Lixia Zhang ,&nbsp;Ruirui Chen ,&nbsp;Xin Li ,&nbsp;Youzhi Feng","doi":"10.1016/j.ejsobi.2024.103708","DOIUrl":"10.1016/j.ejsobi.2024.103708","url":null,"abstract":"<div><div>Climate warming impacts agricultural ecosystems in an unpredictable manner. Below-ground microbes are pivotal for aboveground productivity, but their influences on crop productivity in a warming climate are unknown. We conducted a regional-scale field survey in 253 rice‒wheat rotation systems using bacterial 16S amplicon sequencing and satellite-derived crop net primary productivity (NPP) data to investigate the relationships between soil bacteria and crop NPP under different temperatures. <em>Actinobacteria</em> were identified as the main driver of crop NPP, accounting for 4.2 % of the variation, with summer warming accounting for 11.9 % of the increase in their relative abundance. Summer warming resulted in an increase in antibiotic production genes within <em>Actinobacteria</em>, potentially reducing crop productivity by inhibiting seed germination and root elongation and by suppressing plant growth-promoting microorganisms. Taken together, our study indicates that warmer summers are expected to increase the relative abundance of soil <em>Actinobacteria</em> in rice-wheat rotation systems, which will negatively impact crop NPP due to their production of antibiotics that suppress beneficial plant microbes and/or inhibit crop seed germination and root elongation.</div></div>","PeriodicalId":12057,"journal":{"name":"European Journal of Soil Biology","volume":"124 ","pages":"Article 103708"},"PeriodicalIF":3.7,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143153808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Collembola–Myxomycetes relationships: Spore feeding and coexistence on dead trees","authors":"Michiko Yano ,&nbsp;Taizo Nakamori","doi":"10.1016/j.ejsobi.2024.103705","DOIUrl":"10.1016/j.ejsobi.2024.103705","url":null,"abstract":"<div><div>Dead trees in forests are home to several organisms interacting with each other. However, research on the interactions between Myxomycetes and other organisms has not progressed. This study investigated the interactions between Myxomycetes, which depends on dead trees in forests, and Collembola, which visits their fruiting bodies. It was hypothesized that Collembola may have viable myxomycetes spores inside and outside their bodies and experiments were conducted to determine whether these spores would germinate. The Myxomycetes families Cribrariaceae and Trichiaceae were collected from the study site and Collembola (Isotomidae) were extracted from the fruiting bodies. We waited for the Collembola to excrete and molt, cultured their feces and exuviae separately, observed germination from the spores, and calculated the percentage germination. Myxomycete spores were found in both feces and exuviae. This result suggests an interaction in which Myxomycetes provides fruiting bodies and spores as food for Collembola, and Collembola not only feed on them but also play a role in their dispersal. This study is significant considering it clarifies part of the interaction between Collembola and Myxomycetes. This study applied a novel approach, using a culture method with double-sided tape and slide glass for the long-term culture of feces and exuviae. This study demonstrated the potential for Myxomycetes to disperse spores by utilizing the endozoochory and epizoochory of Collembola. This study discusses the effectiveness of the spore dispersal of Myxomycetes via Collembola.</div></div>","PeriodicalId":12057,"journal":{"name":"European Journal of Soil Biology","volume":"124 ","pages":"Article 103705"},"PeriodicalIF":3.7,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143153812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of heavy metal pollution on soil microorganisms: Influence of soil physicochemical properties. A systematic review","authors":"Claudia Campillo-Cora ,&nbsp;Andrés Rodríguez-Seijo ,&nbsp;Paula Pérez-Rodríguez ,&nbsp;David Fernández-Calviño ,&nbsp;Vanesa Santás-Miguel","doi":"10.1016/j.ejsobi.2024.103706","DOIUrl":"10.1016/j.ejsobi.2024.103706","url":null,"abstract":"<div><div>This review examines the complex interaction between heavy metals and soil microorganisms, focusing on five common heavy metals (HM) (chromium -Cr-, copper -Cu-, nickel -Ni-, lead -Pb-, and zinc -Zn-) in polluted areas worldwide. The systematic review was performed following PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) guidelines. The literature selection procedure involved searching four databases (Web of Science, Scopus, Google Scholar, and PubMed) with a variety of search queries and inclusion and exclusion criteria. As a result of the review, 106 scientific articles that addressed Cr, Cu, Ni, Pb and/or Zn effect on soil microorganisms between 2018 and 2022 were identified. Soil microorganisms, crucial for soil functions/functioning, are impacted by heavy metal pollution, affecting essential functions such as nutrient cycling, organic matter cycling, and carbon sequestration. Various microbial properties (microbial activity -including enzymatic activity-, microbial community composition/diversity, microbial biomass/abundance), reflecting heavy metal effects, show diverse microbial responses influenced by both heavy metal pollution and soil properties (soil pH, organic matter content, texture). Although extensive research has been conducted in this field, further studies are needed to better understand the intricate relationship between heavy metal (HM) pollution, soil microbial responses, and soil properties influence. This review explores the most common methodologies and their main challenges and underscores the need for methodologies to specifically assess HM toxicity. Understanding these details is essential for developing effective strategies to mitigate the adverse effects of HM pollution on soil ecosystems.</div></div>","PeriodicalId":12057,"journal":{"name":"European Journal of Soil Biology","volume":"124 ","pages":"Article 103706"},"PeriodicalIF":3.7,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143153815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Legacy effects of grazing and nitrogen fertilization on soil carbon, nitrogen and phosphorus in an alpine meadow on the Qinghai-Tibetan Plateau","authors":"Lan Li ,&nbsp;Xiong Zhao He ,&nbsp;Yi Sun ,&nbsp;Tianhao Xiao ,&nbsp;Yang Liu ,&nbsp;Fujiang Hou","doi":"10.1016/j.ejsobi.2024.103704","DOIUrl":"10.1016/j.ejsobi.2024.103704","url":null,"abstract":"<div><div>Increasing soil carbon (C) and nitrogen (N) storage can help mitigate climate change and sustain soil fertility. Changes in herbivore and anthropogenic nutrient enrichment intensities can lead to dramatic shifts in the plant and microbial communities, soil organic carbon (SOC) and nutrient dynamics. However, the legacy effects of grazing and N enrichment on the biogeochemical processes remain unclear. Here, we conducted a 6-year rotational grazing (Stocking rates: 0, 8 and 16 sheep ha⁻¹) and 4-year N-addition (N addition levels: 0, 50, 100 and 200 kg N ha⁻¹ yr⁻¹) experiment to investigate how soil C, N and phosphorus (P) components respond to the legacy effects of grazing and N fertilization after a 3-year cessation of grazing and N addition treatments in an alpine meadow on the Qinghai-Tibetan Plateau (QTP). We show that previous grazing significantly increased soil total nitrogen (STN), slightly increased SOC and decreased soil total phosphorus (STP); while previous N fertilization significantly decreased SOC, but it did not significantly alter STN and STP. Previous grazing at low stocking rates (≤ 8 sheep ha⁻¹) might amplify the negative legacy effects of N fertilization on SOC, while a higher stocking rate would weaken the negative impacts of previous N fertilization on SOC. The interactive and synergistic impacts of historical grazing and N fertilization induced a significantly negative effect on STP. Previous N fertilization decreased soil microbial carbon (MBC) and increased soil available N:P, resulting in the reduction of SOC. The increase in plant diversity caused by previous grazing increased SOC, which counteracted the negative effects of increasing bacterial diversity. Previous grazing-induced decreasing bacterial community heterogeneity may lead to increased STN. Although previous grazing-induced increases in soil moisture and soil nutrient availability may have positive effects on STP, previous grazing-induced negative effects on STP may exceed those positive effects. Therefore, the legacy effects of grazing could be beneficial for improving soil C and N, but may increase the risk of soil P loss in the short term, while residual exogenous N could pose a detrimental effect on C storage over time. Reintroducing grazing and/or P addition may be an appropriate choice to offset the adverse consequence of N deposition in the context of global change. Our findings suggest that the stocking rate at about 8 sheep ha⁻¹ could be a suitable grassland management technique for soil fertility sequestration and mitigating the negative influences of residual exogenous N in the QTP.</div></div>","PeriodicalId":12057,"journal":{"name":"European Journal of Soil Biology","volume":"124 ","pages":"Article 103704"},"PeriodicalIF":3.7,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143153814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of nitrogen addition and seasonal changes on moss biocrust soil fungal communities in a temperate desert","authors":"Tingwen Huang ,&nbsp;Xi-En Long ,&nbsp;Weiguo Liu","doi":"10.1016/j.ejsobi.2024.103703","DOIUrl":"10.1016/j.ejsobi.2024.103703","url":null,"abstract":"<div><div>Many studies have reported that changes in nitrogen (N) deposition affect the structure and diversity of fungal communities in moss crust soils, but few studies have addressed the seasonal patterns of soil fungal community response to N inputs in desert habitats. Therefore, we conducted a one-time field N addition experiment in March 2017 in the Gurbantünggüt Desert, northwestern China. Four N addition rates, 0 (CK), 1.8 (LN), 3.6 (MN), and 7.2 (HN) g N m⁻² yr⁻¹, were applied, and soil was sampled at different seasons. We found that the effects of N addition on soil fungal communities varied with season, with stronger effects in November and March compared to May. Seasonal variation strongly affected fungal community structure, composition, and function, with the highest diversity index in May. The impact of N addition on fungal communities is attributed to changes in soil pH, total phosphorus, and available phosphorus, while the effect of season on fungal communities is driven by changes in temperature, soil moisture and soil organic carbon. Additionally, season has a greater effect on fungal communities than N addition. Overall, the fungal communities in soils underlying moss crusts responded strongly to seasonal variation, but their response to N addition was seasonally dependent.</div></div>","PeriodicalId":12057,"journal":{"name":"European Journal of Soil Biology","volume":"124 ","pages":"Article 103703"},"PeriodicalIF":3.7,"publicationDate":"2024-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143153813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Responses of soil microbial community structure under litter to changes in precipitation and nitrogen addition in a desert steppe","authors":"Jinpeng Ma ,&nbsp;Lin Chen ,&nbsp;Danbo Pang ,&nbsp;Yinglong Chen ,&nbsp;Mengyao Wu ,&nbsp;Yaqi Zhang ,&nbsp;Wenqiang He ,&nbsp;Xuebin Li","doi":"10.1016/j.ejsobi.2024.103696","DOIUrl":"10.1016/j.ejsobi.2024.103696","url":null,"abstract":"<div><div>Soil microorganisms are essential in maintaining terrestrial ecosystem function and are central drivers of soil-plant nutrient cycling. However, relatively few studies have explored the impact of precipitation and nitrogen (N) addition on soil microbial community structure beneath litter. In this study, we conducted a field simulation control experiment on litter decomposition under varying precipitation regimes (normal, increased by 30 %, and decreased by 30 %) and N addition levels (0 and 10 g m⁻² y⁻¹) in the desert steppe of Yanchi County, China. Our findings revealed that changes in precipitation and N addition promoted litter decomposition and caused the accumulation of soil nutrients. Specifically, N addition significantly increased nitrate nitrogen (51.95 %), ammonium nitrogen (42.92 %), soil organic carbon (6.81 %), and total phosphorus (7.82 %)(<em>P</em>＜0.05), decreased precipitation significantly elevated contents of nitrate nitrogen (26.80 %), total nitrogen (24.47 %), soil organic carbon (37.62 %), total phosphorus (22.78 %), and microbial biomass C (33.20 %) (<em>P</em>＜0.05). N addition decreased microbial biomarkers content by 1.13 %, but increased microbial diversity indices (<em>Shannon-Wiener</em> index (1.53 %)<em>, Brillouin</em> diversity index (0.54 %)<em>, Pielou</em> evenness index (1.12 %)<em>, Simpson</em> dominance index (0.91 %)<em>, Mcintosh</em> diversity index (1.11 %)) (<em>P</em>＜0.05). Meanwhile, decreased precipitation significantly enhanced microbial biomarkers content by 5.83 % and diversity indices (<em>Shannon-Wiener</em> index (3.67 %)<em>, Brillouin</em> diversity index (2.16 %)<em>, Pielou</em> evenness index (1.55 %)<em>, Simpson</em> dominance index (1.82 %)<em>, Mcintosh</em> diversity index (2.63 %)) (<em>P</em>＜0.05). We indicated the decreased precipitation enhanced the effect of N addition on microbial community and diversity, while increased precipitation showed the opposite trend. Redundancy analysis highlighted MBC as a critical factor influencing microbial community structure, accounting for 35.3 % of the variation (<em>P</em>＜0.01). This study provides valuable insights into managing and conserving desert steppe ecosystems.</div></div>","PeriodicalId":12057,"journal":{"name":"European Journal of Soil Biology","volume":"124 ","pages":"Article 103696"},"PeriodicalIF":3.7,"publicationDate":"2024-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142756786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Coupling methane oxidation and N2 fixation under methanogenic conditions in contrasting environments","authors":"Yongliang Mo ,&nbsp;Jiwei Li ,&nbsp;Xiaotong Peng ,&nbsp;Adrian Ho ,&nbsp;Zhongjun Jia","doi":"10.1016/j.ejsobi.2024.103693","DOIUrl":"10.1016/j.ejsobi.2024.103693","url":null,"abstract":"<div><div>Microbial methane oxidation under widespread suboxic environment is crucial for understanding methane emission. However, the role of aerobic methanotrophs in mediating methane oxidation and nitrogen fixation is less understood in oxygen-limiting environments. In this study, we identified diazotrophic methanotrophs under oxygen-limited conditions (initial O₂ of 6–8 μM) in two contrasting habitats (paddy soil and marine sediment) using DNA-based stable isotope probing combined with amplicon sequencing. Consistently, we documented significant ¹³CH₄ oxidation and ¹⁵N₂ fixation after 740 days of suboxic isotope labeling. Sequencing analysis revealed the predominance of <em>Methylobacter</em>–affiliated aerobic methanotrophs in the ¹³C-labeled DNA fractions. These <em>Methylobacter</em>-like OTUs accounted for 97.86 % in paddy soil and 99.49 % in marine sediment of the total <em>pmoA</em> gene sequences; while relative abundances for the <em>nifH</em> gene sequences were 91.59 % in paddy soil and 99.49 % in marine sediment. Taken together, our analyses revealed that <em>Methylobacter</em> is responsible for methane oxidation and nitrogen fixation under oxygen limitation in both habitats, demonstrating convergent emergence of this aerobic methanotroph under oxygen deficiency.</div></div>","PeriodicalId":12057,"journal":{"name":"European Journal of Soil Biology","volume":"123 ","pages":"Article 103693"},"PeriodicalIF":3.7,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142705591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nutrient supply enhances positive priming of soil organic C under straw amendment and accelerates the incorporation of straw-derived C into organic C pool in paddy soils","authors":"Yuxuan Zhang ,&nbsp;Mengya Lu ,&nbsp;Zhiquan Wang ,&nbsp;Kun Zhang ,&nbsp;Bin Zhang ,&nbsp;Reziwanguli Naimaiti ,&nbsp;Shangyuan Wei ,&nbsp;Xueli Ding","doi":"10.1016/j.ejsobi.2024.103695","DOIUrl":"10.1016/j.ejsobi.2024.103695","url":null,"abstract":"<div><div>Straw return accelerates the decomposition of soil organic C (SOC), a phenomenon referred to as the priming effect. However, the interactive influence of nutrient supply levels on priming effect intensity and SOC sequestration in paddy soils still needs to be better understood. In this study, we investigated the dynamics of the priming effect and associated changes in phospholipid fatty acids, enzyme activity, and microbial necromass following the addition of ¹³C-labelled rice straw (98 % atom) to soils under three nutrient supply levels during a 300-d incubation period. Our results showed that the addition of straw (5 g C kg⁻¹ soil) with no-nutrient (S + Nu₀), low nutrient (S + Nu_low, 42 mg N kg⁻¹, 10 mg P kg⁻¹), and high nutrient (S + Nu_high, 126 mg N kg⁻¹, 30 mg P kg⁻¹) supply increased total CO₂ production by 42.9 %, 59.0 %, and 97.3 %, respectively, compared to the control soil. After 300 d, the cumulative priming effect was nearly doubled in the S + Nu_low and tripled in the S + Nu_high compared to the S + Nu₀. Moreover, the intensity of priming varied with the incubation stage under nutrient treatments. Similar patterns of priming effect were observed across all straw amendments during the early incubation stages; however, the priming effect increased with the nutrient supply levels in the later stages. These patterns are linked to microbial metabolic limitation and resource acquisition strategies, as evidenced by a lower C-to-N stoichiometry of extracellular enzymes and necromass in the S + Nu_low S + Nu_high. A greater proportion of straw-derived C incorporation into SOC (indicated by higher levels ¹³C-SOC) in nutrient-enriched was found, which largely offset the native SOC losses, resulting in high SOC content by the end of incubation. Our findings highlight the critical role of nutrient supply in regulating the priming effect and the balance of SOC after straw return in paddy soils.</div></div>","PeriodicalId":12057,"journal":{"name":"European Journal of Soil Biology","volume":"123 ","pages":"Article 103695"},"PeriodicalIF":3.7,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142652449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In-depth insights into carbohydrate-active enzyme genes regarding the disparities in soil organic carbon after 12-year rotational cropping system field study","authors":"Hao Wang ,&nbsp;JinPing Chen ,&nbsp;Mingxue Du ,&nbsp;Yihao Ruan ,&nbsp;Jiameng Guo ,&nbsp;Ruixin Shao ,&nbsp;Yongchao Wang ,&nbsp;Qinghua Yang","doi":"10.1016/j.ejsobi.2024.103694","DOIUrl":"10.1016/j.ejsobi.2024.103694","url":null,"abstract":"<div><div>Carbohydrate-active enzymes (CAZymes) play a crucial role in plant-derived carbon utilization and decomposition and are influenced by the crop rotation system; however, our knowledge of how different agricultural systems impact CAZyme functionality is still limited. We conducted a metagenomic analysis to evaluate the functional genes of CAZymes in a 12-year in situ farmland with three commonly used crop rotation systems: wheat-maize rotation (WM), wheat-cotton rotation (WC), and wheat-soybean rotation (WS). We aimed to study the impact of long-term use of crop rotation, especially crop rotation involving soybean, on soil organic carbon (SOC) content and to gain an in-depth understanding of the CAZyme genes in context of the disparities in SOC. After 12 years, the SOC content was significantly higher in WS than in WC (5.44 %) and WM (17.6 %). Furthermore, the crop rotation system had a significant effect on the soil microbial communities and CAZyme function genes. Detailly, WS increased the phyla abundance of <em>Proteobacteria</em>, <em>Actinobacteria</em>, and <em>Firmicutes</em> and enriched the glycoside hydrolase (GH) and carbohydrate-binding modules (CBM) genes; WC increased the abundance of <em>Acidobacteria</em> and <em>Bacteroidota</em> and enriched the polysaccharide lyase gene; WM increased the abundance of <em>Nitrospirae</em>, <em>Candidatus_Rokubacteria</em>, <em>Chloroflexi</em> and <em>Gemmatimonadetes</em> and enriched the gene abundance of glycosyltransferases and auxiliary activity genes. Additionally, <em>Acidobacteria</em>, <em>Proteobacteria</em>, and <em>Actinobacteria</em> are key phyla involved in soil carbon cycling and collectively contribute &gt;70 % of the total CAZyme functional genes, which highlights their importance. In addition, our results indicated that total nitrogen content played a major role in influencing genes related to CAZymes, especially those belonging to the GH family. Our study demonstrates that WS conferred the advantage of increasing SOC across the three crop rotation systems. CAZyme analysis revealed that WS's could potentially support the increased abundance of <em>Proteobacteria</em>, <em>Actinobacteria</em> and <em>Firmicutes</em> in the soil community, at the same time potentially leading to increased number of GH and CBM genes in the soil, which may bolster the decomposition and transformation of plant-derived carbon, thus promoting an increase in SOC content. The findings of this study offer new insights into the microbial factors contributing to SOC enhancement in rotation systems.</div></div>","PeriodicalId":12057,"journal":{"name":"European Journal of Soil Biology","volume":"123 ","pages":"Article 103694"},"PeriodicalIF":3.7,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142652448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}