European Journal of Medical Research最新文献

{"title":"The potential of MCM8 as a biomarker in esophageal carcinoma: a comprehensive analysis integrating m6a methylation and angiogenesis.","authors":"Xu-Sheng Liu, Yong Xu, Li-Song Dai, Ao Li, Jie Liu, Yu Zhang, Jin Xie, Zhi-Jun Pei","doi":"10.1186/s40001-025-02541-3","DOIUrl":"https://doi.org/10.1186/s40001-025-02541-3","url":null,"abstract":"<p><strong>Background: </strong>Effective biomarkers for esophageal carcinoma (ESCA) are currently lacking. Here, we examined the role of minichromosome maintenance complex component 8 (MCM8) as a diagnostic and prognostic marker in ESCA and its association with m6a methylation and angiogenesis, and constructed a competing endogenous RNA (ceRNA) network.</p><p><strong>Methods: </strong>Clinical data and gene expression profiles were obtained from The Cancer Genome Atlas and Gene Expression Omnibus datasets. Differential gene expression analysis was performed using DESeq2 and limma packages. The prognostic significance of MCM8 expression regarding overall survival (OS) was examined using the Cox proportional hazards model. Receiver Operating Characteristic (ROC) analysis was used to assess the diagnostic potential of MCM8. MCM8 expression in ESCA tissues was evaluated by immunohistochemical staining on a tissue microarray. Pearson correlation analysis identified co-expressed genes, followed by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses. The GEPIA online tool was used to examine the relationship between MCM8 and m6a methylation as well as angiogenesis-related genes. MicroRNA and long noncoding RNA predictions were made using miRWalk, MicroT-CDS, ENCORI, and miRNet tools to construct the ceRNA network.</p><p><strong>Results: </strong>MCM8 was significantly overexpressed in tumor tissues and showed high diagnostic accuracy in the ROC analysis with an area under the curve of 0.920. Kaplan-Meier survival analysis revealed that high MCM8 expression correlated with poorer OS and disease-specific survival. Pearson correlation analysis identified a significant correlation between MCM8 and several m6a methylation-related genes such as HNRNPA2B1 and YTHDF1, as well as PTK2, an angiogenesis-related gene. A ceRNA network including MCM8, PURPL/hsa-miR- 135a- 5p/MCM8 was successfully predicted and constructed.</p><p><strong>Conclusions: </strong>MCM8 is a promising biomarker in ESCA and it is associated with m6a methylation and angiogenesis, showing potential as a therapeutic target. The ceRNA network provided insight into the pathogenesis of ESCA.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"373"},"PeriodicalIF":2.8,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12063286/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143998726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thirty-day mortality risk prediction for geriatric patients undergoing non-cardiac surgery in the surgical intensive care unit.","authors":"Mengke Ma, Jiatong Liu, Caiyun Li, Yingxue Chen, Huishu Jia, Aijie Hou, Hongzeng Xu","doi":"10.1186/s40001-025-02543-1","DOIUrl":"https://doi.org/10.1186/s40001-025-02543-1","url":null,"abstract":"<p><strong>Background: </strong>The prediction of mortality for elderly patients undergoing non-cardiac surgeries is a vital research area, as accurate risk assessment can help surgeons make better clinical decisions during the perioperative period. This study aims to build a mortality risk prediction model for surgical intensive care unit (ICU) patients aged 65 and older undergoing non-cardiac surgery.</p><p><strong>Methods: </strong>Data was obtained from 1960 patients who underwent non-cardiac surgery from the medical information mart for intensive care IV (MIMIC-IV) database. The least absolute shrinkage selection operator (LASSO) regularization algorithm and the extreme gradient boosting (XGBoost) for feature importance evaluation were used to screen important predictors. Five predictive models were established: categorical boosting (CatBoost), logistic regression (LR), decision tree (DT), random forest (RF), and support vector machine (SVM). External validation was performed utilizing data from 153 patients in the MIMIC-III database. Finally, shapley additive explanations (SHAP) was utilized for a personalized analysis of the models.</p><p><strong>Results: </strong>Among the five predictive models developed in this study, the CatBoost model demonstrated superior overall performance in both the test data set (AUC = 0.96, F1 = 0.90) and the external validation data set (AUC = 0.98, F1 = 0.91). The decision curve analysis showed that the model offers a beneficial net benefit. The CatBoost model showed significant enhancements in classification accuracy when compared to the conventional revised cardiac risk index (RCRI) score. SHAP analysis revealed that anion gap, age, prothrombin time (PT), and weight were the four key variables influencing the predictive performance of the CatBoost model.</p><p><strong>Conclusions: </strong>This study demonstrates the potential of machine learning methods for early prediction of outcomes in critically ill elderly patients undergoing non-cardiac surgery. A web-based application was developed, which could serve as an effective tool for clinicians in their risk assessment and clinical decision-making processes.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"372"},"PeriodicalIF":2.8,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12063265/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143975929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The relationship between the triglyceride-glucose index and functional outcomes in patients with aneurysmal subarachnoid hemorrhage: a retrospective cohort study.","authors":"Yuyang Hou, Xinyi Guo, Hongkuan Yang, Hua Li, Rudong Chen, Xiaoli Min, Jiasheng Yu","doi":"10.1186/s40001-025-02629-w","DOIUrl":"https://doi.org/10.1186/s40001-025-02629-w","url":null,"abstract":"<p><strong>Background: </strong>Aneurysmal subarachnoid hemorrhage (aSAH) is a life-threatening disease with high morbidity and mortality. The triglyceride-glucose (TyG) index, a marker of insulin resistance (IR), has been linked to adverse outcomes in cerebrovascular conditions; however, its influence on functional prognosis in aSAH remains unclear. This study aimed to elucidate the relationship between the TyG index and functional outcomes in aSAH patients.</p><p><strong>Methods: </strong>A retrospective cohort study included consecutive aSAH patients. Functional outcomes were assessed using the modified Rankin Scale (mRS) at 3 months and categorized as favorable (mRS 0-2) or unfavorable (mRS 3-6). Univariate and multivariate logistic regression analyzed the association between the TyG index and functional outcomes. Propensity score matching (PSM) was used to mitigate confounding. Non-linear relationships were explored with restricted cubic splines (RCS), and subgroup analyses were performed. A nomogram integrating the TyG index and traditional prognostic scales was developed, and model predictive performance was compared using the area under the curve (AUC) on a test set.</p><p><strong>Results: </strong>A total of 470 patients (61.7% female) were enrolled, with 154 experiencing unfavorable outcomes. Multivariate logistic regression showed a significant association between the TyG index and adverse outcomes (OR: 1.86, 95% CI 1.12-3.1, P = 0.017). An optimal TyG index cutoff of 8.83 was identified. Patients with TyG index ≥ 8.83 had a higher risk of poor outcomes (48.7% vs. 24.8%; P = 0.015). PSM confirmed these findings. RCS indicated a progressive association between elevated TyG index and increased risk of adverse functional outcome. Subgroup analyses showed consistent relationships. The enhanced model with the TyG index had a higher AUC (0.899) than the traditional model (0.889, DeLong test P = 0.048).</p><p><strong>Conclusions: </strong>A high TyG index is significantly associated with an increased risk of unfavorable functional outcomes in patients with aSAH.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"375"},"PeriodicalIF":2.8,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12063392/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143957722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Concomitant body-wide trauma patterns in patients with head and neck injuries: a comparison based on the trauma register DGU® by the German trauma society and the dortmund maxillofacial trauma registry.","authors":"Ákos Bicsák, Fatma Topcuoglu, Stefan Hassfeld, Rolf Lefering, Lars Bonitz, Jens-Peter Stahl","doi":"10.1186/s40001-025-02636-x","DOIUrl":"https://doi.org/10.1186/s40001-025-02636-x","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Introduction: &lt;/strong&gt;Interdisciplinary trauma patients with multiple injuries require special attention from the first minute of care. This study aimed to determine the overall injury distribution in patients with head and neck injury (HNI) with respect to the head and neck injury location.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;Data on patients with HNI were collected in the Dortmund Maxillofacial Trauma Register 2007-2017 based on a review of patient radiographs and files. All patients with concomitant injuries to central body parts were selected and analysed further by an expert traumatologist collecting data on GCS, ISS, injuries and outcome. Further comparisons with data from the German TraumaRegister DGU® 2007-2022 were acquired and used to determine whether the patient group with head and neck injuries differed from other injured patients.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;A total of 70.212 patients with head and neck injuries were identified from the 344.754 reported cases in the TraumaRegister DGU®. Among the 344,754 patients, 6127 were registered with mandibular injuries (1.8% of the total population/8.7% of patients with HNI), panfacial injuries were reported in 10,001 patients (2.9%/14.2%), and midfacial injuries were reported in 42,045 patients (12.2%/59.9%). Injuries to the thorax (39.5% of non-HNI patients vs. 35.4% of HNI patients), abdomen (10.8% vs. 6.7% of HNI patients), and extremities (25.6% non-HNI vs. 20.1% HNI, respectively), spine (29.8% non-HNI patients vs. 27.0% HNI patients), lower extremities (26.1% vs. 22.3%, respectively), and iliac bone (16.5% non-HNI patients vs. 13.6% HNI patients) and upper extremities (29.0% non-HNI patients and 33.8% HNI patients). A total of 289 severely injured patients from 7010 head and neck injuries were selected from the Dortmund Maxillofacial Trauma Register. The mechanism of the injuries included falls (n = 143; 49.5%), road traffic accidents (75, 26.0%), interpersonal violence (48, 16.6%), work-related accidents (14, 4.8%), sports accidents (7, 2.4%) and other causes (2, 0.7%). No in-hospital deaths were reported in this population. Seven of the 289 patients (2.4%) needed reanimation upon arrival at the emergency room. In this group, the average ISS = 50.3 (Injury Severity Score), and the GCS = 7.1 (Glasgow Coma Scale). Six of the 7 patients had a calvarial fracture (86%), and injuries to the cervical, thoracic and lumbar spine were detected in 1, 2 and 2 patients (14%, 28% and 28%, respectively). Ventilation was applied in 4 patients (57%). Overall, most soft tissue injuries were identified in the face (128) and head areas (57), followed by the thorax and back areas (54 and 28). The most joint injuries were found in the hip, shoulder and radiocarpal and hand regions (11, 10 and 10 injuries, respectively). Most cases were considered severe. The body-wide fracture distribution was similar: most fractures occurred in the calvaria (227) or thoracic area (27 multiple rib fractures and","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"371"},"PeriodicalIF":2.8,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12060487/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143991572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression and prognostic value of estrogen-related receptor β alternative splicing isoforms in esophageal squamous cell carcinoma.","authors":"Kai-Yuan Jiang, Jia-Xin Wan, Hong-Yun Li, Wei-Yang Chen, Chun-Mei Shen, Ke-Xuan Guo, Xiang-Yun Zheng, Hong-Ying Wen, Dong Tian","doi":"10.1186/s40001-025-02638-9","DOIUrl":"https://doi.org/10.1186/s40001-025-02638-9","url":null,"abstract":"<p><strong>Background: </strong>Estrogen-related receptor β (ERRβ) alternative splicing isoforms, including ERRβsf, ERRβ2, and ERRβΔ10, have been implicated in the pathogenesis of malignant tumors. Nevertheless, their specific impact on esophageal squamous cell carcinoma (ESCC) remains unclear. The study aimed to investigate the expression and prognostic value of ERRβ alternative splicing isoforms in ESCC.</p><p><strong>Methods: </strong>This study prospectively collected ESCC tissues and paired normal tissues of 54 patients with ESCC who underwent esophagectomy without neoadjuvant therapy. The protein expression levels of ERRβ alternative splicing isoforms in ESCC and normal tissues were detected by Western blot. The Kaplan-Meier method with a log-rank test was used to estimate overall survival (OS). The Cox proportional hazards regression analysis was used to evaluate the independent prognostic factors.</p><p><strong>Results: </strong>In ESCC tissues, the ERRβsf/ERRβ ratio was significantly higher (P = 0.017) compared to paired normal tissues. Based on a cut-off value of 0.24, there were 18 and 36 cases in the high ERRβsf/ERRβ expression group and low ERRβsf/ERRβ expression group, respectively. Patients with high ERRβsf/ERRβ ratios had significantly better OS than those patients with low ERRβsf/ERRβ ratios (77.1% vs 50.8%, P = 0.024). The multivariate analysis revealed that ERRβsf/ERRβ (hazard ratio [HR] = 0.219, 95% confidence interval [CI] 0.063-0.767, P = 0.018) and N stage (HR = 7.892, 95% CI 1.328-46.911, P = 0.023) were independent prognostic factors for ESCC patients.</p><p><strong>Conclusions: </strong>This study is the first to demonstrate the relationship between ERRβ alternative splicing isoforms in ESCC. A high ERRβsf/ERRβ ratio was associated with a better prognosis, indicating that ERRβ alternative splicing isoforms may serve as potential prognostic biomarkers for ESCC.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"369"},"PeriodicalIF":2.8,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12056985/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143993409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanisms through which laparoscopic sleeve gastrectomy mitigates atherosclerosis risk: a focus on visceral adipose tissue.","authors":"Juan Xu, Heyue Wang, Bin Han, Xiaomin Zhang","doi":"10.1186/s40001-025-02635-y","DOIUrl":"https://doi.org/10.1186/s40001-025-02635-y","url":null,"abstract":"<p><p>Bariatric surgery is currently considered the key treatment method for patients with obesity and related complications. Among the various surgeries, laparoscopic sleeve gastrectomy (LSG) is the most widely used. Obesity is a multifactor chronic disease characterized by the accumulation of visceral adipose tissue (VAT), leading to susceptibility to cardiac metabolic diseases. Many mechanisms, including abnormal lipid metabolism, insulin resistance, inflammation, endothelial dysfunction, adipocytokine imbalance and inflammasome activation, have been identified as the basis for the relationship between obesity and atherosclerosis. Bariatric surgery, such as LSG, reduces the risk of atherosclerosis in people living with obesity by reducing energy intake, disrupting energy balance and reducing the secretion of intestinal hormones to intervene in these risk factors. This review explores the current understanding of how LSG affects VAT and its impact on the risk of atherosclerosis.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"370"},"PeriodicalIF":2.8,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12057030/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143989616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Risk factors for postoperative complications in patients with pulmonary tuberculosis.","authors":"Bing Wang, Li Yao, Jian Sheng, Xiaoyu Liu, Yuhui Jiang, Lei Shen, Feng Xu, Qibin Liu, Sheng Bao, Chao Gao, Xiyong Dai","doi":"10.1186/s40001-025-02633-0","DOIUrl":"https://doi.org/10.1186/s40001-025-02633-0","url":null,"abstract":"<p><strong>Background: </strong>The risk factors associated with postoperative complications following pulmonary resection in individuals with tuberculosis remain incompletely understood.</p><p><strong>Methods: </strong>We conducted a retrospective analysis of baseline data-including sex, age, BMI, comorbidities, previous COVID-19 status, smoking history, respiratory function, ASA grade, affected lung lobe, and operative factors-in patients who underwent surgical treatment at Wuhan Pulmonary Hospital between January 2018 and September 2022.</p><p><strong>Results: </strong>This study included 204 patients diagnosed with pulmonary tuberculosis (PTB) who underwent surgery at our hospital between January 2018 and September 2022. Of these, 138 cases (67.6%) were male and the median age was 49 years. Postoperative complications were observed in 63 patients, representing an incidence rate of 30.9% (63/204). The most commonly reported complications were prolonged air leak (PAL; 29 cases), postoperative pleural effusion (PE; 23 cases), post-resectional space (PRS; 27 cases), pneumonia (9 cases), and hemorrhage (5 cases). Multivariate analysis identified male sex (odds ratio [OR]: 2.322, 95% confidence interval [CI] 1.015-5.313, p = 0.046), severe adhesion grade (OR 4.304, 95% CI 1.710-10.830, p = 0.002), and longer operative time (OR 1.007, 95% CI 1.003-1.011; p = 0.001) as significant risk factors for postoperative complications. For PAL specifically, male sex (OR 4.003, 95% CI 1.111-14.421, p = 0.034), severe adhesion grade (OR 3.943, 95% CI 1.313-11.839, p = 0.014), and longer operative time (OR 1.005, 95% CI 1.001-1.009, p = 0.016) were significant risk factors. Significant risk factors for postoperative PE included severe adhesion grade (OR 6.078, 95% CI 1.318-28.026, p = 0.021) and longer operative time (OR 1.005, 95% CI 1.000-1.010, p = 0.043). Blood transfusion (OR 4.493, 95% CI 1.270-15.888, p = 0.020) was identified as a significant risk factor for PRS.</p><p><strong>Conclusions: </strong>Male gender, severe adhesions, and prolonged operative time were identified as significant risk factors for postoperative complications. Specifically, risk factors for postoperative PAL included male sex, severe adhesions, and longer operative time. Severe adhesions and prolonged operative time were also associated with an increased risk of postoperative PE. Intraoperative blood transfusion emerged as a significant risk factor for PRS. This finding helps us identify problems, improve operations, and reduce potential postoperative complications.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"367"},"PeriodicalIF":2.8,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12057153/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143990767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Topical tranexamic acid prevents scar tissue formation following craniectomy in a rat model.","authors":"Ömer Şahin, Tuncer Taşcıoğlu, Ayşegül Fırat, Hüseyin Selçuk Sürücü, Muzaffer Çaydere","doi":"10.1186/s40001-025-02634-z","DOIUrl":"https://doi.org/10.1186/s40001-025-02634-z","url":null,"abstract":"<p><strong>Background: </strong>We carried out a study to assess the efficacy of tranexamic acid in preventing scar tissue in the craniectomy area in rats.</p><p><strong>Method: </strong>Our study consisted of control and tranexamic acid groups with 10 subjects each. All subjects underwent bilateral frontoparietal craniectomy. After craniectomy, cotton pads were applied to the surgical sites. In the controls, the pads were soaked with saline and in the tranexamic acid group the pads were soaked with 30 mg/kg tranexamic acid. Rats were decapitated 30 days after surgery. The degree of scar formation was evaluated pathologically and by electron microscopy. In pathologic evaluation, dura mater thickness, scar tissue density, and arachnoid involvement were evaluated.</p><p><strong>Results: </strong>The outcomes demonstrated that no adhesions were present in the rats of the Tranexamic acid group, whereas the control group exhibited severe scar tissue [eight of ten rats (80%)] with adhesions. Additionally, comparison between the two groups showed that the dura mater thickness of tranexamic acid animals was thinner than that of the control group animals. Similarly, the intensity of scar tissue density and the intensity of arachnoid involvement were much better than the control group.</p><p><strong>Conclusions: </strong>Scar tissue formation following craniectomies represents a significant adverse outcome that may lead to various complications. Intraoperative topical application of tranexamic acid has demonstrated potential efficacy in preventing scar formation in the craniectomy region in rat models.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"366"},"PeriodicalIF":2.8,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12057060/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143997998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spatial transcriptomic characteristics of gastric cancer in young and the expression and role of TMEM176B in gastric cancer cells.","authors":"Zhenhua Jiang, Yongfeng Wang, Chi Zhang, Shuangyin Han","doi":"10.1186/s40001-025-02577-5","DOIUrl":"https://doi.org/10.1186/s40001-025-02577-5","url":null,"abstract":"<p><strong>Background: </strong>Gastric cancer in young (GCY) is increasing in incidence with poor prognosis. Current screening and molecular methods are inadequate, necessitating new approaches to explore its pathogenesis. This study used spatial transcriptomic sequencing (ST-seq) to analyze the cellular composition of gastric cancer (GC) tumors, compare gene expression patterns, explore signaling pathways, and investigate the role of the differentially expressed gene (DEG) TMEM176B in GCY.</p><p><strong>Methods: </strong>The surgical specimens of six patients with GCY were included to construct a tissue microarray containing the tumor core region (TCR), cancer-adjacent tissue (CAT), and normal gastric tissue (NGT). ST-seq was performed to obtain the transcript expression levels at different spatial locations. After quality control, normalization, standardization, clustering, dimensionality reduction, and cell-type prediction analyses were carried out to identify the DEGs. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses were used to clarify the underlying mechanisms of GC. Based on the results, TMEM176B was selected for functional analysis. Western blotting was used to assess TMEM176B expression in normal gastric cells and cancerous cells. shRNA-mediated TMEM176B knockdown in cancer cells was used for phenotypic analysis, proliferation assays, and apoptosis experiments.</p><p><strong>Results: </strong>This study identified heterogeneous cell populations in GCY tissues. Exactly 18,082 DEGs were found between the TCR and CAT, mainly enriched in the IL-17, AGE-RAGE, and relaxin pathways. Moreover, 17,586 DEGs were identified between the TCR and NGT, primarily related to the HIF-1 and apoptosis pathways. TMEM176B was a key DEG in the TCR vs. CAT and TCR vs. NGT comparisons. It was highly expressed in GCY tissues and GC cell lines. Further analysis using The Cancer Genome Atlas database confirmed its oncogenic effects. TMEM176B knockdown in GC cell lines inhibited cell proliferation (reduced CCK8 and colony formation), increased apoptosis (higher Bax/Bcl2 ratio), and arrested the cell cycle in the G0/G1 phase.</p><p><strong>Conclusions: </strong>This study used ST-seq to map the transcriptomic profiles of the TCR, CAT, and NGT in patients with GCY, investigating gene spatial expression patterns and tumor heterogeneity. We identified TMEM176B's role in GC development and progression, offering molecular targets and a foundation for future treatments.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"368"},"PeriodicalIF":2.8,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12057093/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143977602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sex-specific association between atherogenic index of plasma and risk of newly diagnosed abdominal aortic aneurysm: a large population-based cohort study.","authors":"Peng Qiu, Hongbin Guo, Chao Zhu, Yijun Liu, Jiazhen Zheng, Hongji Pu, Xinwu Lu, Qun Huang, Guang Liu, Kaichuang Ye, Zhen Zhou","doi":"10.1186/s40001-025-02586-4","DOIUrl":"https://doi.org/10.1186/s40001-025-02586-4","url":null,"abstract":"<p><strong>Objectives: </strong>Atherosclerosis of aortic wall has been suggested as a key pathological feature of abdominal aortic aneurysm (AAA). We conducted a first-ever prospective cohort study aiming at assessing the sex-specific association between atherogenic index of plasma (AIP) and risk of newly diagnosed AAA.</p><p><strong>Methods: </strong>This study included 193,013 male and 226,785 female participants from the UK Biobank. AIP was calculated as a ratio of logarithmically transformed triglycerides to high-density lipoprotein-cholesterol. The outcome of interest was new AAA, identified by ICD-10 and OPCS-4 code, or by AAA-related death. All analyses were sex-stratified: Multivariable Cox proportional-hazard models were employed to assess the association between baseline AIP and AAA risk. Harrell's c index was estimated to assess the value of AIP added to the discrimination of AAA prediction model.</p><p><strong>Results: </strong>Over an average follow-up of 15.3 years, 1931 (1.00%) new AAA cases were recorded in males and 424 (0.19%) in females. In the fully adjusted models, compared with the bottom AIP quintile, HRs (95% CI) of newly diagnosed AAA was 1.67 (1.41, 1.96) in males and 1.75 (1.22, 2.52) in females within the top quintile. Subgroup analysis found smoking status significantly modified the association in females, with association existing only in female ever-smokers. Adding AIP into prediction model comprising age, smoking, and CVD history significantly improved the discrimination in males and male high-risk subgroups and in female ever-smokers (p < 0.05).</p><p><strong>Conclusions: </strong>This study highlights the potential of AIP as a biomarker for AAA and its utility in identifying high-risk individuals qualified for AAA screening.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"363"},"PeriodicalIF":2.8,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12054325/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143974321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}