European Journal of Medical Research最新文献

{"title":"Comparison of effect of remimazolam and propofol on respiration of patients under deep sedation for colonoscopy: a prospective multicenter randomized controlled trial.","authors":"Zhengjia Wang, Renshu Zhan, Liqun Mo, Jin Zhang, Jie Hu, Shoupeng Tan, Qiongzhen He, Ping Li, Wekong Sun, Xiaobin Wang, Jun Jiang, Li Liu, Yingying Zhang, Yiping Bai","doi":"10.1186/s40001-025-02519-1","DOIUrl":"10.1186/s40001-025-02519-1","url":null,"abstract":"<p><strong>Background: </strong>Remimazolam recently became available as a sedative. The comparison of the respiratory suppression effects of remimazolam and propofol under deep sedation for colonoscopy was not thoroughly unclear, particularly with regard to the novel metric of time to first airway intervention. The goal of this study was to systemically compare the respiration profiles of the patients sedated with remimazolam and propofol at the comparable sedation level in the patients undergoing colonoscopy.</p><p><strong>Methods: </strong>Four hundred-fifty outpatients were randomly assigned to remimazolam (Group Rem, n = 225) and propofol (Group Pro, n = 225). The target sedation level was the modified Observer's Assessment of Alertness/Sedation ≤ 2. The primary outcome was elapsed time from anesthesia induction to first airway intervention. Secondary outcomes included incidence and severity of hypoxia and apnea, minute ventilation (MV), tidal volume (TV), and respiratory rate (RR).</p><p><strong>Results: </strong>The elapsed time from induction to the first airway intervention was 11 ± 8 min in Group Rem (n = 208) vs. 5 ± 6 min in Group Pro (n = 208, P < 0.001). Patients in Group Rem required less frequent airway intervention and had a lower incidence of and shorter duration of apnea than patients in Group Pro (all P < 0.001). MV at 1 min, 2 min, 4 min post-induction, and at the end of the procedure were higher in Group Rem than those in Group Pro (P < 0.001).</p><p><strong>Conclusions: </strong>Patients sedated with remimazolam vs. propofol during colonoscopy maintain improved respiration and require less frequent airway intervention, and have lower incidence of adverse events. Clinical trial registration and registry URL ChiCTR2000034527, registered at www.chictr.org.cn.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"250"},"PeriodicalIF":2.8,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11974117/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143794979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical-genomic characteristics of homologous recombination deficiency (HRD) in breast cancer: application model for practice.","authors":"Jinsui Du, Lizhe Zhu, Chenglong Duan, Nan Ma, Yudong Zhou, Danni Li, Jianing Zhang, Jiaqi Zhang, Yalong Wang, Xi Liu, Yu Ren, Bin Wang","doi":"10.1186/s40001-025-02520-8","DOIUrl":"10.1186/s40001-025-02520-8","url":null,"abstract":"<p><strong>Background: </strong>Homologous recombination deficiency (HRD) affects breast cancer patients. Treatment guided by multigene testing may be particularly beneficial in HRD patients by using platinum-based drugs and poly ADP-ribose polymerase inhibitor (PARPi). However, the optimal method for HRD testing remains undetermined by guidelines or consensus and economic disparities limit the availability of genetic testing. Prioritizing HRD testing by clinical-genomic characteristics is critical for efficient utilization of healthcare resources and improved treatment accuracy.</p><p><strong>Methods: </strong>A total of 93 breast cancer patients who underwent HRD genetic testing were included in the study. According to the machine learning model called genomic scar (GS) HRD was defined as a genomic scar score (GSS) ≥ 50 or with deleterious mutation in the BRCA. Multivariate logistic regression analysis was employed to identify the clinical-pathological factors potentially associated with HRD. Suitable variables were selected to construct a predictive model, and the model's efficacy was evaluated using the area under the receiver operating characteristic (ROC) curve. Internal validation was performed using bootstrap resampling (500 replicates).</p><p><strong>Results: </strong>Patients harboring pathogenic mutation in BRCA exhibited higher GSS (99.85 vs 36.90). HRD was not detected in 41.75% of patients, and 34.95% had HRD but no BRCA pathogenic mutations. HRD risk in human epidermal factor growth receptor 2 (HER2) low or positive was significantly lower compared to HER2 negative (OR: 0.390, 95% CI: 0.159-0.959, P = 0.040). High Ki- 67 index was strongly associated with HRD (OR: 28.434, 95% CI: 3.283-246.293, P = 0.002). Significant variations in GSS were observed based on estrogen receptor (ER) and progesterone receptor (PR) status, histological grade, and molecular types. The area under the ROC curve (AUC) of the combined prediction model combining HER2 status and Ki- 67 index was 0.749, and the accuracy of the model was further validated using bootstrap resampling (500 replicates), resulting in an AUC of 0.730, indicating a high predictive accuracy for HRD status.</p><p><strong>Conclusions: </strong>BRCA mutation status did not fully reflect HRD status. Patients with a negative HER2 status and high Ki- 67 index are more likely to exhibit positive results when undergoing HRD genetic testing. The ER, PR, HER- 2 status, Ki- 67 index, molecular typing, and histological grading may have a strong influence on the HRD status.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"249"},"PeriodicalIF":2.8,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11974012/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143794957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent advances in 3D printing applications for CNS tumours.","authors":"Wireko Andrew Awuah, Simran Karkhanis, Adam Ben-Jaafar, Jonathan Sing Huk Kong, Krishitha Meenu Mannan, Princess Afia Nkrumah-Boateng, Joecelyn Kirani Tan, Adeleke Oluwaseun Dorcas, Vallabh Shet, Muhammad Hamza Shah, Toufik Abdul-Rahman, Oday Atallah","doi":"10.1186/s40001-025-02483-w","DOIUrl":"10.1186/s40001-025-02483-w","url":null,"abstract":"<p><p>Three-dimensional printing (3DP) has emerged as a transformative technology in the field of central nervous system (CNS) tumours, offering innovative advancements in various aspects of diagnosis, treatment and education. By precisely replicating the microenvironment of CNS tumours, modelling tumour vascularisation, and capturing genetic heterogeneity, 3DP enables the development of targeted therapies and personalised treatment strategies. The technology has markedly enhanced preoperative planning and intraoperative guidance, providing highly accurate, patient-specific models that improve tumour localisation, facilitate tailored surgical planning, and offer superior visualisation of complex anatomical structures. Furthermore, 3DP has revolutionised education and training for neurosurgeons, trainees, and patients by delivering realistic simulations that enhance surgical skills and decision-making. Despite its transformative potential, the widespread adoption of 3DP faces challenges, including material biocompatibility issues, high costs, and technical limitations. Furthermore, the ethical and regulatory landscape for 3DP in clinical practice requires further development. This review concludes that while 3DP offers significant promise for advancing CNS tumour treatment, ongoing research is essential to address these challenges and optimising its clinical impact.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"251"},"PeriodicalIF":2.8,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11974138/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143794981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The potential role of immune environment in dental patients treated with PMMA-based dental interim prosthetic materials.","authors":"Jiayi Chen","doi":"10.1186/s40001-025-02536-0","DOIUrl":"10.1186/s40001-025-02536-0","url":null,"abstract":"","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"255"},"PeriodicalIF":2.8,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11974197/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143795024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasonic measurement of acetabular alpha angle to evaluate developmental dysplasia of the hip: differences between AIUM guideline and European consensus.","authors":"Ying Zhao, Yumiao Qiao, Kai Hong, Chao Zhang","doi":"10.1186/s40001-025-02530-6","DOIUrl":"10.1186/s40001-025-02530-6","url":null,"abstract":"<p><strong>Purpose: </strong>To clarify the difference between the alpha angles measured by European method and by AIUM method.</p><p><strong>Materials and methods: </strong>In this prospective study, infants less than 6-month-old with clinical suspicion of DDH were enrolled from the pediatric outpatient service in a large provincial teaching hospital from October 2022 to October 2023. The hips were examined according to the protocols described by AIUM guideline and by European consensus. The acetabular alpha angle was measured on the standard sections. Statistically significant difference between groups was determined by using a paired-sample t test.</p><p><strong>Results: </strong>A total of 225 infants (median age, 4.0 months [interquartile range, 3.3-5.2 months]; 104 boys) were enrolled into the study. The mean value of alpha angle measured by AIUM method (62.1°; 95% CI 61.7, 62.4) was smaller than that by European method (63.4°; 95% CI 63.1, 63.7) (P < 0.0001). The 95% limits of agreement between alpha angles measured by the two methods were - 6.6 to 9.2 degree. Alpha angle measured by AIUM method could diagnose DDH with low sensitivity (56% [9 of 16 hips; 95% CI 31, 79]) and specificity (78% [300 of 384 hips; 95% CI 74, 82]) with Graf's technique as a reference standard. The position of transducer arranged on the skin of infant by AIUM method was 1.6 ± 0.3 cm back to the position by European method.</p><p><strong>Conclusion: </strong>Alpha angle measured by AIUM method is smaller than that by European method and should not be applied to Graf typing of DDH.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"254"},"PeriodicalIF":2.8,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11974195/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143795026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The metabolites of gut microbiota: their role in ferroptosis in inflammatory bowel disease.","authors":"Jingying Zhou, Penghui Lu, Haolong He, Ruhan Zhang, Dican Yang, Qiong Liu, Qianyan Liu, Mi Liu, Guoshan Zhang","doi":"10.1186/s40001-025-02524-4","DOIUrl":"10.1186/s40001-025-02524-4","url":null,"abstract":"<p><p>Inflammatory bowel disease (IBD) includes chronic inflammatory conditions, such as Crohn's disease and ulcerative colitis, characterized by impaired function of the intestinal mucosal epithelial barrier. In recent years, ferroptosis, a novel form of cell death, has been confirmed to be involved in the pathological process of IBD and is related to various pathological changes, such as oxidative stress and inflammation. Recent studies have further revealed the complex interactions between the microbiome and ferroptosis, indicating that ferroptosis is an important target for the regulation of IBD by the gut microbiota and its metabolites. This article reviews the significant roles of gut microbial metabolites, such as short-chain fatty acids, tryptophan, and bile acids, in ferroptosis in IBD. These metabolites participate in the regulation of ferroptosis by influencing the intestinal microenvironment, modulating immune responses, and altering oxidative stress levels, thereby exerting an impact on the pathological development of IBD. Treatments based on the gut microbiota for IBD are gradually becoming a research hotspot. Finally, we discuss the potential of current therapeutic approaches, including antibiotics, probiotics, prebiotics, and fecal microbiota transplantation, in modulating the gut microbiota, affecting ferroptosis, and improving IBD symptoms. With a deeper understanding of the interaction mechanisms between the gut microbiota and ferroptosis, it is expected that more precise and effective treatment strategies for IBD will be developed in the future.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"248"},"PeriodicalIF":2.8,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11974165/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143795022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Risk factors for and a preliminary prediction model of coronary artery calcification in patients beginning hemodialysis.","authors":"Hao Xiong, Cuifang Sun, Jie Song, Yan Yu, Chang Wang, Fang Yuan","doi":"10.1186/s40001-025-02523-5","DOIUrl":"10.1186/s40001-025-02523-5","url":null,"abstract":"<p><strong>Background and hypothesis: </strong>Vascular calcification (VC) is an important risk factor for cardiovascular events in patients undergoing maintenance hemodialysis (MHD); however, there is limited data on VC-related factors in patients beginning hemodialysis. Thus, this study aimed to determine the risk factors of VC and to establish a prediction model for evaluating VC progression in new patients undergoing hemodialysis.</p><p><strong>Methods: </strong>This study selected 86 patients who initiated in-center MHD between March 2021 and November 2022. Demographic characteristics, medical history, and laboratory data were collected. Coronary artery calcification (CAC) was assessed based on the Agatston vascular score determined via computed tomography. Serum levels of the VC inhibitors fetuin-A was quantified via enzyme-linked immunosorbent assays. Univariate and multivariate regression analyses were conducted to determine the risk factors for VC, and a neural network-based approach was adopted to construct a VC prediction model.</p><p><strong>Results: </strong>The average age of the patients was 56.74 ± 12.79 years, and 65.1% were male. CAC was observed in 72.09% of patients. Age, body mass index, diabetes, the comorbidity index, and the number of coronary artery branches with calcification were positively correlated with the CAC score, whereas plasma fetuin-A levels was negatively correlated. The multivariate logistic regression analysis revealed that age [odds ratio (OR) 1.07, 95%CI 1.00-1.14], the comorbidity index [OR 1.72, 95%CI 1.16-2.57], diabetes [OR 3.97, 95%CI 1.16-13.58] were independent risk factors for CAC; these factors were used to establish a simple scoring model to predict VC risk.</p><p><strong>Conclusion: </strong>Age, the comorbidity index, diabetes were identified as independent risk factors for CAC in patients beginning hemodialysis, and the new VC prediction model based on these factors may help identify VC in patients undergoing MHD, facilitating clinical interventions.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"252"},"PeriodicalIF":2.8,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11974132/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143794982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"U‑shaped association between relative fat mass (RFM) and stress urinary incontinence: a cross‑sectional study.","authors":"Jiaqing Yang, Yuanzhuo Du, Ju Guo","doi":"10.1186/s40001-025-02481-y","DOIUrl":"10.1186/s40001-025-02481-y","url":null,"abstract":"<p><strong>Background: </strong>This study aimed to investigate the relationship between relative fat mass (RFM) and stress urinary incontinence (SUI).</p><p><strong>Methods: </strong>This cross-sectional study employed data from the National Health and Nutrition Examination Survey (NHANES), collected from 2005 to 2018. Weighted logistic regression and smooth curve fitting were employed to evaluate the association between RFM and SUI. Subgroup analyses and interaction tests were performed to validate the robustness of the findings. The predictive effect was evaluated using receiver operating characteristic (ROC) curves. Finally, we analyzed the role of RFM in predicting SUI using the Random Forest Variable Importance plot and SHAP Dependence Plot.</p><p><strong>Results: </strong>Among 32,594 participants aged 20 years and older, 22.94% were diagnosed with SUI. The fully adjusted multivariable model indicated that a higher RFM was associated with an increased risk of developing SUI (OR = 2.42; 95% CI 2.05-2.86). Subgroup analysis and interaction tests were performed to validate this association further. Smoothing curve fitting revealed a U-shaped relationship between RFM and SUI. The ROC curve demonstrated that RFM (AUC = 0.788, 95% CI 0.782-0.793) is a good predictor of SUI. Lastly, the Random Forest Variable Importance plot and SHAP Dependence Plot effectively identified the positive correlation and non-linear relationship between SUI and RFM.</p><p><strong>Conclusion: </strong>A non-linear correlation was observed between elevated RFM and the incidence of SUI. Especially within the female population, an increase in RFM is related to a higher likelihood of SUI, indicating that RFM could be a possible tool for identifying SUI.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"256"},"PeriodicalIF":2.8,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143802820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Risk factors for enteral nutrition-associated diarrhea in older patients with severe traumatic brain injury: a retrospective cohort study.","authors":"Xiao-Xiao Shi, Qiao-Ling Zhang, Qian Li, Xiao-Dan Lu, Li Fan, Jia-Jiang Jiang, Xin-Yi Tu, Qiu-Si Hu, Li-Zhu Wang","doi":"10.1186/s40001-025-02504-8","DOIUrl":"10.1186/s40001-025-02504-8","url":null,"abstract":"<p><strong>Background: </strong>Severe traumatic brain injury (STBI) is one of the major causes of death and disability worldwide. The incidence and risk factors of enteral nutrition (EN)-associated diarrhea in older patients with STBI remain unclear.</p><p><strong>Methods: </strong>A cohort of adult STBI patients were retrospectively studied. The patients were stratified into an older group (≥ 65years) and a young group (< 65 years). All patients received EN for at least 48 h. Demographic, clinical and nutritional data were collected for analysis. We utilize multiple logistic regression models to evaluate predictors of diarrhea.</p><p><strong>Results: </strong>Among 292 patients with STBI aged 60.38 ± 14.89 years (mean ± standard deviation), 114 cases developed diarrhea, with an incidence of 39.04%. Older patients had a higher incidence of diarrhea than young patients (46.77% vs 33.33%, p = 0.020). Three variables were found to be significantly associated with diarrhea in young STBI patients. In contrast, five variables were significantly associated with this complication in older STBI patients, including acute physiology and chronic health evaluation II score (adjusted OR 1.134, 95% CI 1.019-1.272, p = 0.025), high-fat energy (adjusted OR 1.221, 95% CI 1.055-1.789, p = 0.025), EN duration (adjusted OR 1.105, 95% CI 1.005-1.223, p = 0.044), antibiotics total defined daily dose (DDDs) (adjusted OR 1.076, 95% CI 1.029-1.211, p = 0.039) and tube feeding of potassium (adjusted OR 2.525, 95% CI 1.031-6.450, p = 0.046).</p><p><strong>Conclusions: </strong>Enteral nutrition-associated diarrhea was prevalent among STBI patients. Older STBI patients had a higher incidence of diarrhea and more risk factors than young patients. Early management of modifiable risk factors may help reduce the incidence of diarrhea.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"253"},"PeriodicalIF":2.8,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11974193/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143795020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of thyroid cancer biomarkers using WGCNA and machine learning.","authors":"Gaofeng Hu, Wenyuan Niu, Jiaming Ge, Jie Xuan, Yanyang Liu, Mengjia Li, Huize Shen, Shang Ma, Yuanqiang Li, Qinglin Li","doi":"10.1186/s40001-025-02466-x","DOIUrl":"10.1186/s40001-025-02466-x","url":null,"abstract":"<p><strong>Objective: </strong>The incidence of thyroid cancer (TC) is increasing in China, largely due to overdiagnosis from widespread screening and improved ultrasound technology. Identifying precise TC biomarkers is crucial for accurate diagnosis and effective treatment.</p><p><strong>Methods: </strong>TC patient data were obtained from TCGA. DEGs were analyzed using DESeq2, and WGCNA identified gene modules associated with TC. Machine learning algorithms (XGBoost, LASSO, RF) identified key biomarkers, with ROC and AUC > 0.95 indicating strong diagnostic performance. Immune cell infiltration and biomarker correlation were analyzed using CIBERSORT.</p><p><strong>Results: </strong>Four key genes (P4HA2, TFF3, RPS6KA5, EYA1) were found as potential biomarkers. High P4HA2 expression was associated with suppressed anti-tumor immune responses and promoted disease progression. In vitro studies showed that P4HA2 upregulation increased TC cell growth and migration, while its suppression reduced these activities.</p><p><strong>Conclusion: </strong>Through bioinformatics and experimental validation, we identified P4HA2 as a key potential thyroid cancer biomarker. This finding provides new molecular targets for diagnosis and treatment. P4HA2 has the potential to be a diagnostic or therapeutic target, which could have significant implications for improving clinical outcomes in thyroid cancer patients.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"244"},"PeriodicalIF":2.8,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11971869/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143788234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}