European Journal of Medical Research最新文献

{"title":"The multidimensional role of SLC16A4 in hepatocellular carcinoma in silico analysis: prognostic significance, metabolic pathways, and immune microenvironment regulation.","authors":"Lili Jiang, Yi Shen, Zhengyang Feng, Yong Wang, Yaqun Zhu, Yuntian Shen, Qiliang Peng","doi":"10.1186/s40001-025-03275-y","DOIUrl":"https://doi.org/10.1186/s40001-025-03275-y","url":null,"abstract":"<p><strong>Background: </strong>This study aims to analyze the expression patterns, clinical relevance, and biological functions of SLC16A4 across multiple cancer types, with a focus on liver hepatocellular carcinoma (LIHC) in silico analysis.</p><p><strong>Methods: </strong>We conducted a pan-cancer analysis using data from TCGA and GTEx databases to evaluate SLC16A4 expression in various cancer types. The LIHC cohort was stratified based on SLC16A4 expression levels for further clinicopathological and survival analysis. Functional enrichment, immune infiltration, and RNA modification correlations were explored using bioinformatics tools, including GO, KEGG, GSEA, and ssGSEA.</p><p><strong>Results: </strong>In LIHC, SLC16A4 was markedly downregulated in tumor tissues compared to normal tissues, and its low expression correlated with advanced pathologic stages and poor histologic grades. Survival analysis revealed that high SLC16A4 expression was associated with improved overall survival and disease-specific survival in LIHC, with multivariate analysis confirming its role as an independent prognostic factor. Functional enrichment analysis linked SLC16A4 to key metabolic pathways. In addition, SLC16A4 expression was correlated with RNA modification genes and mismatch repair genes, suggesting its involvement in post-transcriptional regulation and genomic stability. Immune infiltration analysis revealed that high SLC16A4 expression was associated with increased infiltration of immune cells and regulating immune-related molecules, indicating its potential role in modulating the tumor immune microenvironment.</p><p><strong>Conclusions: </strong>SLC16A4 exhibits cancer-specific expression patterns and plays a multifaceted role in tumor progression, metabolism, and immune regulation. Its potential as a diagnostic and prognostic biomarker, particularly in LIHC, warrants further investigation. These findings highlight SLC16A4 as a promising target for future cancer research and therapy.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"1007"},"PeriodicalIF":3.4,"publicationDate":"2025-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145344294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decoding dynamic lipase trajectory patterns and in-hospital mortality in acute pancreatitis: insights from machine learning in intensive care units.","authors":"Yingyi Li, Xiaoqiang Liu, Xiaodong Zhu, Chanchan Lin, Qilin Yang, Zicheng Huang, Yisen Huang","doi":"10.1186/s40001-025-03299-4","DOIUrl":"10.1186/s40001-025-03299-4","url":null,"abstract":"<p><strong>Background: </strong>Serum lipase levels are crucial biomarkers in acute pancreatitis (AP), yet their dynamic patterns and prognostic implications remain incompletely understood. This study aimed to identify distinct lipase trajectory phenotypes and evaluate their association with in-hospital mortality in AP patients.</p><p><strong>Methods: </strong>We conducted a retrospective analysis of 834 AP patients from the MIMIC-IV database using latent class trajectory modeling (LCTM) to identify distinct lipase trajectory phenotypes. Cox regression models, adjusted for demographics, comorbidities, clinical therapies, and critical illness markers, were employed to assess the association between trajectory classes and in-hospital mortality.</p><p><strong>Results: </strong>Three distinct lipase trajectory phenotypes were identified: Class 1 (n = 543) with consistently low levels, Class 2 (n = 51) with extremely high and variable levels, and Class 3 (n = 240) with moderately elevated levels. Class 2 patients were significantly older (66.8 ± 17.6 years) and had higher comorbidity burden (CCI: 5.6 ± 3.0). In-hospital mortality rates were 12.2%, 17.6%, and 19.2% for Classes 1, 2, and 3, respectively. After comprehensive adjustment, both Class 2 (HR: 2.21, 95% CI 1.04-4.71, p = 0.042) and Class 3 (HR: 1.61, 95% CI 1.08-2.40, p = 0.022) showed significantly higher mortality risk compared to Class 1.</p><p><strong>Conclusions: </strong>Dynamic lipase trajectory patterns in AP patients demonstrate distinct phenotypes with significant prognostic value for in-hospital mortality. These findings suggest that monitoring lipase trajectories may enhance risk stratification and guide clinical management in AP patients.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"1011"},"PeriodicalIF":3.4,"publicationDate":"2025-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12542429/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145343933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel inflammation biomarkers in adult minimal change disease: predicting steroid-resistance and relapse.","authors":"Weiwei Zhang, Wei Lin, Huipeng Ge, Wenbin Tang, Zhangzhe Peng, Qiongjing Yuan, Xiangcheng Xiao","doi":"10.1186/s40001-025-03279-8","DOIUrl":"https://doi.org/10.1186/s40001-025-03279-8","url":null,"abstract":"<p><strong>Introduction: </strong>The novel systemic inflammation markers are a class of indicators combining clinically common laboratory indices, reflecting the underlying immune and inflammatory status. Minimal change disease (MCD) is an important cause of idiopathic nephrotic syndrome (INS) in adults. Novel systemic inflammation markers were evaluated for their ability to predict treatment response to steroids and subsequent relapse in adult-onset MCD.</p><p><strong>Methods: </strong>This unicentric, retrospective study included the clinical data of adult-onset INS patients who were pathologically diagnosed with MCD at Xiangya Hospital of Central South University from January 2010 to December 2021 and followed up with the patients' remission after adequate steroid treatment, starting from the time of initial complete remission to May 31, 2022, with recurrence as the end-point event. Eight common and associated novel systemic inflammation markers were collected, and univariate analysis of these markers was performed using dummy variable assignment or maximally selected rank statistics. Multivariate logistic regression and Cox regression statistics identified the independent risk factors for steroid resistance and relapse after initial remission in adult-onset MCD, respectively.</p><p><strong>Results: </strong>A total of 121 patients were included; the median age was 22 (19,40) years, and there were 92 (76%) men. Adequate corticosteroids were the initial treatment: 98 (81%) patients achieved remission, and 23 (19%) developed steroid resistance. Of the 98 patients with remission, the median age was 25 (19, 44) years, 76 (77.6%) were male, the median follow-up time was 11.4 (6.3, 33.4) months, and 46 (46.9%) experienced relapse. The multivariate analysis showed that elevated C-reactive protein to albumin ratio (CAR) (≥ 0.196) and derived neutrophil/ (leukocyte minus neutrophil) ratio (dNLR) (≥ 1.32) were independent predictors of steroid resistance and relapse in adult-onset MCD, respectively.</p><p><strong>Conclusions: </strong>The novel systemic inflammation markers CAR and dNLR may play significant roles in steroid treatment and the prognosis of MCD in adults, and deeper clinical studies in the future are warranted.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"1008"},"PeriodicalIF":3.4,"publicationDate":"2025-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145344297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bruceine D inhibits hepatocellular carcinoma by regulating the STAT3 signaling pathway through HSP70.","authors":"Pu Chen, Qing Xu, Shuling Wu, Fanyun Zhu, Yi Zhou, Qingqing Lin, Min Zhao, Ju Yang, Haiyang Zhao, Bin Zhou, Chengguang Zhao","doi":"10.1186/s40001-025-03270-3","DOIUrl":"https://doi.org/10.1186/s40001-025-03270-3","url":null,"abstract":"<p><strong>Background: </strong>Hepatocellular carcinoma (HCC) is a leading cause of cancer-related mortality worldwide, with limited effective therapies and poor prognosis. Although sorafenib and lenvatinib are approved for advanced HCC, their clinical efficacy is often compromised by drug resistance and adverse effects. Bruceine D (BD) has shown antitumor potential in several cancers, but its mechanisms in HCC remain poorly defined.</p><p><strong>Methods: </strong>The effects of BD on HCC cell proliferation, apoptosis, and migration were assessed by MTT, colony formation, EdU incorporation, flow cytometry, wound healing, and transwell assays. Differentially expressed genes were identified by RNA sequencing and validated by RT-qPCR, with particular focus on Hsp70 family members. Protein expression and interactions were analyzed using Western blotting, co-immunoprecipitation (Co-IP), and Cellular Thermal Shift Assay (CETSA). Molecular docking was performed to predict BD binding sites on Hsp70. The antitumor efficacy and safety of BD were further evaluated in BALB/c nude mouse xenograft models.</p><p><strong>Results: </strong>BD significantly inhibited HCC cell growth, induced apoptosis, and suppressed migration in a dose-dependent manner in vitro study. Transcriptomic profiling revealed downregulation of Hsp70 family members (HspA1A, HspA1B, HspA8), confirmed by RT-qPCR and Western blotting. Molecular docking suggested hydrogen bond interactions of BD with multiple residues in the Hsp70 domain. Co-IP assays demonstrated that Hsp70 binds STAT3, and BD disrupted this interaction, resulting in reduced STAT3 phosphorylation and suppression of downstream effectors (MCL-1, survivin). CETSA proved BD protected HSP70 at various temperature gradients in HCC cells. In vivo, BD markedly reduced tumor volume without affecting body weight or causing histopathological abnormalities in major organs.</p><p><strong>Conclusions: </strong>This study demonstrates that BD exerts potent antitumor effects against HCC by inhibiting proliferation, inducing apoptosis, and suppressing migration, primarily through disruption of the Hsp70/STAT3 signaling axis. With its favorable safety profile, BD represents a promising candidate for further development as a therapeutic agent in HCC.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"1010"},"PeriodicalIF":3.4,"publicationDate":"2025-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145344411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dysregulation of mineral metabolism: association of albumin-corrected calcium and vitamin D with diabetic kidney disease in type 2 diabetes-a cross-sectional study.","authors":"Qiong Ma, Xue Li, Lin Liu, Fuqiang Liu, Ying Lv, Junkui Wang, Ting Wang","doi":"10.1186/s40001-025-03277-w","DOIUrl":"https://doi.org/10.1186/s40001-025-03277-w","url":null,"abstract":"<p><strong>Background: </strong>Disturbances in calcium (Ca), vitamin D, and electrolyte balance have been implicated in diabetic kidney disease (DKD), yet existing evidence remains inconsistent. Moreover, the role of albumin-corrected Ca-a more accurate measure of biologically active Ca in patients with frequent hypoalbuminemia-remains poorly defined in type 2 diabetes mellitus (T2DM). We aimed to investigate the association between albumin-corrected Ca, vitamin D, electrolytes and DKD in a large T2DM cohort.</p><p><strong>Methods: </strong>In this cross-sectional study, 5550 T2DM patients were enrolled, including 1574 participants with DKD. Multivariable regression analyses were used to assess the association between albumin-corrected Ca, vitamin D and DKD. Spearman's correlation analyses were conducted to examine the correlation between electrolyte levels, vitamin D and indicators of renal impairment.</p><p><strong>Results: </strong>Patients with DKD in T2DM were older, predominantly male, with longer diabetes duration, higher blood pressure, poorly glycemic control and more diabetic complications compared to those without DKD. Notably, patients with DKD also showed significant electrolyte imbalances, suggesting dysregulated mineral metabolism. Multivariate logistic regression revealed albumin-corrected Ca (odds ratio [OR]: 4.032, 95% confidence interval [CI]: 2.017-8.062, P < 0.001), 1,25-(OH)₂-VitD₃ (OR: 0.967, 95% CI: 0.960-0.974, P < 0.001) were independently associated with DKD after accounting for confounding factors.</p><p><strong>Conclusions: </strong>We demonstrated that elevated albumin-corrected Ca and decreased 1,25-(OH)₂-VitD₃ were significantly associated with DKD in T2DM patients. These findings suggest the potential of mineral metabolism parameters, particularly albumin-corrected Ca, to enhance early detection and risk stratification. Future prospective cohort studies are essential to establish a causal relationship and clarify their clinical therapeutic guidance value.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"1009"},"PeriodicalIF":3.4,"publicationDate":"2025-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145343939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and validation of a risk prediction model for co-existing depression in middle-aged and older adults with low back pain.","authors":"Kaixia Gao, Meichi Yan, Jinmeng Tao, Jian Shi, Chen Gong, Haozhi Zhao, Junting You, Beibei Feng, Yuling Wang","doi":"10.1186/s40001-025-03281-0","DOIUrl":"https://doi.org/10.1186/s40001-025-03281-0","url":null,"abstract":"<p><strong>Background: </strong>Low back pain (LBP), a prevalent disorder among middle-aged and older adults, imposes substantial medical and socioeconomic burdens on individuals and society. Worse still, comorbid depression is frequently present in chronic LBP, which aggravates the functional prognosis. It is of significance to identify relevant predictors associated with the occurrence of depression in LBP. Therefore, this study developed a risk prediction model to estimate depression risk in LBP patients, which may support its early detection and intervention.</p><p><strong>Methods: </strong>This study used representative data from the China Health and Retirement Longitudinal Study. Thirty-one candidate variables encompassing socio-demographic, pain-related, behavioral, health status, and psychological factors were analyzed. Participants were randomly split into the training and validation cohorts (7:3 ratio). LASSO regression with tenfold cross-validation was used to select predictors. A logistic regression model was constructed, and a nomogram was developed based on the final predictors. Model performance was evaluated by area under the receiver operating characteristic curve (AUC), calibration plots, and decision curve analysis (DCA).</p><p><strong>Results: </strong>A total of 1,693 participants with LBP from 2018 to 2020 were included, with a depression incidence of 29.4%. Multivariable logistic regression identified predictors of depression in LBP, involving gender, education, sleep quality, chronic diseases, life satisfaction, cognitive function, pain severity, use of analgesics, and among others. The nomogram demonstrated good discrimination (AUC 0.736 and 0.718 in the training and validation set, respectively). Hosmer-Lemeshow tests indicated a good model fit (P > 0.05). DCA confirmed favorable clinical utility.</p><p><strong>Conclusion: </strong>The developed model provides a practical tool for assessing the risk of depression in middle-aged and older adults with LBP, supporting early identification and targeted preventive strategies in clinical practice.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"1006"},"PeriodicalIF":3.4,"publicationDate":"2025-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145343942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sarcopenic obesity exacerbates gastric cancer prognosis via increased intermuscular and visceral fat accumulation.","authors":"Chun-Wei Li, Qi Li, Yu Liu, Yu Zhang, Zhong-Kui Wang, Yu Xing, Yi-Shan Niu, Yi Pang, Kang Yu","doi":"10.1186/s40001-025-03251-6","DOIUrl":"https://doi.org/10.1186/s40001-025-03251-6","url":null,"abstract":"<p><strong>Background: </strong>Ectopic fat distribution reflects patient's metabolic profile, shaping the tumor microenvironment, which may be a key prognostic indicator. Our study pursues to explore the independent and combined effects of muscle atrophy and abnormal fat distribution on survival in gastric cancer patients.</p><p><strong>Method: </strong>This retrospective cohort study analyzed body composition in 283 gastric cancer patients from Center Hospital, Tianjin University (June 2016-May 2025) using pretreatment CT at L₃, assessing muscle mass, intermuscular, and visceral fat. Ectopic fat phenotypes included visceral obesity (increased visceral  adipose tissue, VAT) and myosteatosis (reduced skeletal muscle density, SMD). Associations between body composition parameters/phenotypes and progression-free survival (PFS), overall survival (OS), and cachexia-related survival (CRS) were evaluated using Kaplan-Meier analysis with log-rank tests and Cox proportional hazards regression.</p><p><strong>Results: </strong>Of the 283 patients, 32 patients (11.3%) were BMI-define obese, 139 patients (49.1%) showed visceral obesity, and 176 patients (62.2%) presented with myosteatosis. Sarcopenia was present in 234 patients (82.7%), and 151 patients (53.4%) presented with a combination of sarcopenia and myosteatosis. During a median follow-up of 41 months (2-108 months), 53 patients developed metastasis, 6 relapse, and 142 died. Skeletal muscle mass (Normal Attenuation Muscle Area, NAMA, HR = 0.989, 95% CI 0.980-0.998, P = 0.022, for PFS) and quality (SMD, HR = 0.975, 95% CI 0.955-0.995, P = 0.013, for PFS) serve as protective prognostic factors after multiple covariate adjustments. Multivariate analyses identified all adiposity-related parameters, including Low Attenuation Muscle Area (LAMA, HR = 1.015, 95% CI 1.003-1.026, P = 0.012, for PFS), intermuscular adipose tissue (IMAT, HR = 1.018, 95% CI 1.002-1.034, P = 0.027, for PFS), and VFI (HR = 1.008, 95% CI 1.001-1.015, P = 0.042) as negative indicators for survival. Sensitivity analyses confirmed these associations. Besides, IMAT, LAMA, and VFI significantly influence prognosis in advanced patients (TNM stage III-IV). Kaplan-Meier survival analysis revealed sarcopenic visceral obesity markedly reduces survival; when combined with myosteatosis (SMVO), this combined phenotype further exacerbated CRS (5-year CRS: 74.7% in SMVO, P = 0.019) beyond the effects of individual phenotypes.</p><p><strong>Conclusion: </strong>Sarcopenic obesity (myosteatosis/visceral obesity) can identify poorer prognosis in gastric cancer patients, especially male patients. It is crucial for obese gastric cancer patients to evaluate their body composition, specifically focusing on fat distribution.</p><p><strong>Trial registration: </strong>Registered in August 2016, NCT02873676 at www.</p><p><strong>Clinicaltrials: </strong>gov .</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"1012"},"PeriodicalIF":3.4,"publicationDate":"2025-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145344306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tdrd15 is dispensable for male fertility and spermatogenesis in the golden hamster.","authors":"Miao Zhu, Yijian Xiang, Haodong Wang, Shanmeizi Zhao, Wentao Zeng, Jianmin Li, Bing Yao","doi":"10.1186/s40001-025-03285-w","DOIUrl":"https://doi.org/10.1186/s40001-025-03285-w","url":null,"abstract":"<p><p>Tudor domain-containing proteins (TDRDs) constitute an evolutionarily conserved protein family and are critical for germline development and piRNA pathway regulation, with established roles in male fertility. While multiple TDRD family members have been functionally linked to spermatogenic impairment, the precise biological role of TDRD15 remains to be elucidated. We used CRISPR/Cas9-mediated gene editing to generate Tdrd15 knockout (KO) golden hamsters (Mesocricetus auratus), a model necessitated by the absence of a functional Tdrd15 ortholog in the mouse genome, to investigate its function in male reproduction. Phylogenetic analysis demonstrated that TDRD15 is strongly conserved among eutherian mammals, with testis-restricted expression patterns in hamsters. Despite the successful induction of frameshift mutations and significant transcriptional knockdown, Tdrd15 KO males maintained normal fertility parameters, including unaltered testicular architecture, spermatogenic progression (confirmed by periodic acidic-Schiff (PAS) staining and immunohistochemistry), and sperm quality metrics determined using a computer-assisted analysis. Quantitative polymerase chain reaction (qPCR) revealed compensatory overexpression of paralogous Tdrd genes in KO testes, implying functional redundancy within this protein family. This study provides the first experimental evidence that TDRD15 is dispensable for male fertility in golden hamsters under physiological conditions, thereby challenging the prevailing assumptions of its obligatory function in spermiogenesis. Altogether, these findings support a more targeted allocation of research efforts within the field of male reproductive biology.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"1013"},"PeriodicalIF":3.4,"publicationDate":"2025-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145344339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the role of lumbar intervertebral space morphology: a novel classification and its impact on cage migration in lumbar fusion surgery.","authors":"Yang Hou, Lei Liu, Tianyi Zhao, Yongfei Guo, Jiangang Shi","doi":"10.1186/s40001-025-03261-4","DOIUrl":"10.1186/s40001-025-03261-4","url":null,"abstract":"<p><strong>Purpose: </strong>This study aimed to establish a novel classification system for lumbar intervertebral spaces based on anatomical morphology, to investigate the relationship between intervertebral space types and cage migration risk after transforaminal lumbar interbody fusion (TLIF), and to optimize fusion strategies tailored to different anatomical types.</p><p><strong>Methods: </strong>A retrospective cohort study was conducted on 103 patients who underwent TLIF between February 2022 and June 2023. Lumbar intervertebral spaces were classified into 3 fundamental types: lordotic, neutral and kyphotic and 2 modified types: normal and degenerative. Clinical outcomes (Visual Analogue Scale [VAS], Oswestry Disability Index [ODI]), and radiographic parameters (Intervertebral Space Angle [ISA], Intervertebral Space Height [ISH], Intervertebral Foramen Height [IFH]) were evaluated. Statistical analyses included t tests, ANOVA, chi-square tests, and logistic regression.</p><p><strong>Results: </strong>VAS and ODI scores significantly improved postoperatively (P < 0.05). ISA, ISH, and IFH showed significant postoperative increases across all fundamental types. Cage migration occurred in 8 segments, predominantly in degenerative-type intervertebral spaces. Logistic regression revealed that the degenerative type was associated with a 17-fold increased risk of cage migration (OR = 17.02). No significant association was found between fundamental type and migration. Fusion rates exceeded 98% across all groups.</p><p><strong>Conclusion: </strong>The novel classification of lumbar intervertebral spaces effectively predicts cage migration risk. The degenerative modified type is a strong risk factor for migration. Tailoring surgical strategies to intervertebral space morphology may improve stability and outcomes in lumbar interbody fusion procedures.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"1003"},"PeriodicalIF":3.4,"publicationDate":"2025-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12542467/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145344312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TIGD6 in gastric cancer: exploring its prognostic value and therapeutic potential through molecular and clinical investigations.","authors":"Quanli Han, Muhong Deng, Zhi Cui, Qi Wang, Dongbing Li","doi":"10.1186/s40001-025-03219-6","DOIUrl":"10.1186/s40001-025-03219-6","url":null,"abstract":"<p><strong>Background: </strong>Gastric cancer (GC) predominantly contributes to cancer mortality, with adenocarcinomas accounting for more than 95% of incidences. Early detection improves survival, but most cases are diagnosed at advanced stages due to subtle symptoms and rapid progression. The role of TIGD6 in GC is unclear.</p><p><strong>Methods: </strong>We analyzed TIGD6 expression using TCGA data and correlated it with clinical features and outcomes in GC patients. Bioinformatics tools, including GSEA and single-cell sequencing, were used to elucidate TIGD6's role in GC. In the GC cell lines AGS and HGC-27, TIGD6 was knocked down using RNA interference, and subsequent in vitro experiments were conducted to evaluate cell proliferation, migration, and invasion capabilities.</p><p><strong>Results: </strong>The expression of TIGD6 was markedly elevated in GC tissues relative to normal tissues (p < 0.001). Higher TIGD6 levels were linked to residual tumors (p = 0.027), history of reflux (p = 0.019), and antireflux treatment (p = 0.0012). Increased TIGD6 expression was associated with decreased overall survival (OS, p = 0.009) and disease-specific survival (DSS, p = 0.008), and it served as an independent predictor of worse OS (p = 0.043). Knocking down TIGD6 in GC cells suppressed proliferation, migration, and invasion, while enhancing apoptosis through modulation of the Hedgehog signaling pathway.</p><p><strong>Conclusion: </strong>TIGD6 is overexpressed in GC and linked to unfavorable outcomes. It could potentially function as a biomarker and therapeutic target for this malignancy. Future studies should validate its clinical relevance and explore its detailed molecular mechanisms. Collectively, this study provides the first functional, mechanistic, and immune-phenotypic characterization of TIGD6 in GC, positioning it as a dual biomarker and therapeutic target.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"1001"},"PeriodicalIF":3.4,"publicationDate":"2025-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12538967/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145344327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}