Ying Guo, Hong Wang, Nu Mi, Qianmin Chen, RongRong Wang, Hui Ni
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引用次数: 0
Abstract
Objectives: This study controlled experimental and histological study based on an ovariectomized (OVX) rat model, and aimed to clarify the distribution changes of TRAP-positive osteoclasts and sclerostin-positive osteocytes in the alveolar septal area of rats after Eldecalcitol (ELD) treatment, and to explore their association with bone metabolism-related factors, thereby preliminarily elucidating the early pathological mechanism of alveolar osteoporosis.
Methods: A total of 32 female Wistar rats (8 weeks) were randomly divided into three groups: sham-operated control (Sham, n = 10), ovariectomized (OVX, n = 11), and ovariectomized treated with Eldecalcitol (OVX + ELD, n = 11). Observations were Made at different timepoints, including 1 week and 2 weeks after surgery. OVX rat models were established by bilateral ovariectomy. Eldecalcitol (0.03 µg/kg/day) was administered orally for 2 weeks starting immediately after surgery. Mandibular samples were fixed, decalcified, and prepared into thin sections for histological analyses. Osteocyte distribution and characteristics were studied in detail using TRAP staining, ALP/TRAP staining, and sclerostin/TRAP/silver triple staining techniques. In addition, double staining of RANKL and TRAP was performed to further explore the expression of bone resorption-related molecules.
Results: The experimental results showed that in the bone groove interval area, rats that underwent ovariectomy (OVX) had a small number of TRAP-positive osteoclasts in the early stage (1 week), but this number gradually increased over time. In the OVX group after 1 week of combined treatment with Eldecalcitol, the number of TRAP-positive osteoclasts was significantly reduced. In addition, the expression of sclerostin-positive osteocytes decreased over time, but this decrease was smaller in the combination treatment group.
Conclusions: This study reveals the distribution of TRAP-positive osteoclasts and sclerostin-positive osteocytes in the alveolar septal area and their correlation with bone metabolism-related factors. Eldecalcitol treatment effectively inhibits osteoclast activity, reduces bone resorption, and significantly mitigates the reduction of sclerostin expression in osteocytes induced by ovariectomy. These findings suggest that Eldecalcitol may regulate alveolar bone metabolism partially by modulating sclerostin expression, making it a promising therapeutic strategy for osteoporosis.
目的：本研究以去卵巢（OVX）大鼠模型为基础，对照实验和组织学研究，旨在阐明Eldecalcitol （ELD）治疗后大鼠肺泡间隔区trap阳性破骨细胞和硬化蛋白阳性骨细胞的分布变化，并探讨其与骨代谢相关因素的关系，从而初步阐明肺泡骨质疏松的早期病理机制。方法：32只雌性Wistar大鼠（8周龄）随机分为3组：假手术对照组（Sham, n = 10）、去卵巢组（OVX, n = 11）和去卵巢组（OVX + ELD, n = 11）。在术后1周和2周的不同时间点进行观察。采用双侧卵巢切除术建立OVX大鼠模型。术后立即口服埃地骨糖醇（0.03µg/kg/天）2周。下颌标本固定，脱钙，制备成薄片用于组织学分析。使用TRAP染色、ALP/TRAP染色和硬化蛋白/TRAP/银三重染色技术详细研究骨细胞分布和特征。此外，通过RANKL和TRAP双染色进一步探讨骨吸收相关分子的表达。结果：实验结果显示，在骨沟间隔区，卵巢切除术（OVX）大鼠在早期（1周）有少量trap阳性破骨细胞，但随着时间的推移，该数量逐渐增加。OVX组联合Eldecalcitol治疗1周后，trap阳性破骨细胞数量明显减少。此外，硬化蛋白阳性骨细胞的表达随着时间的推移而下降，但这种下降在联合治疗组较小。结论：本研究揭示了trap阳性破骨细胞和硬化蛋白阳性骨细胞在肺泡间隔区的分布及其与骨代谢相关因素的相关性。Eldecalcitol治疗有效抑制破骨细胞活性，减少骨吸收，并显著减轻卵巢切除术诱导的骨细胞中硬化蛋白表达的减少。这些发现表明，骨糖醇可能通过调节硬化蛋白的表达来部分调节牙槽骨代谢，使其成为治疗骨质疏松症的一种有希望的策略。
期刊介绍：
European Journal of Medical Research publishes translational and clinical research of international interest across all medical disciplines, enabling clinicians and other researchers to learn about developments and innovations within these disciplines and across the boundaries between disciplines. The journal publishes high quality research and reviews and aims to ensure that the results of all well-conducted research are published, regardless of their outcome.
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