European Journal of Medical Research最新文献

{"title":"Risk analysis of the association between EASIX and all-cause mortality in critical ill patients with atrial fibrillation: a retrospective study from MIMIC-IV database.","authors":"Yu Xia, Anfeng Liang, Mei Wang, Jianlin Zhang","doi":"10.1186/s40001-025-02621-4","DOIUrl":"https://doi.org/10.1186/s40001-025-02621-4","url":null,"abstract":"<p><strong>Background: </strong>The Endothelial Activation and Stress Index (EASIX) is a recognized marker of vascular endothelial health but has limited application in patients with atrial fibrillation (AF). This study aimed to explore the association between EASIX and prognosis in critically ill patients with AF.</p><p><strong>Methods: </strong>The patient's data were extracted from Medical Information Mart for Intensive Care IV(MIMIC-IV) database. EASIX was calculated as lactate dehydrogenase (U/L) × creatinine (mg/dL)/platelets (10⁹ cells/L) and log2-transformed for statistical analysis. The Boruta algorithm and Least Absolute Shrinkage and Selection Operator (Lasso) Regression were used for feature selection. Multivariable logistic regression and Cox proportional hazard models were employed to assess EASIX as a risk factor, with nonlinear relationships evaluated using restricted cubic spline curves. The area under the receiver operating characteristic curve (AUC) was utilized to compare the predictive performance of EASIX with the Sequential Organ Failure Assessment (SOFA) score and the CHA₂DS₂-VASc score. Furthermore, Kaplan-Meier survival analysis based on EASIX quartiles (with Q1 as the reference) and stratified analyses were conducted to further explore these associations.</p><p><strong>Results: </strong>A total of 4896 patients with complete data were included. In-hospital, 28-day, and 365-day all-cause mortality rates were26.04%, 29.25%, and 49.75%, respectively. The median EASIX was 5.64 (4.56, 6.84). Higher EASIX was significantly associated with increased in-hospital, short-term, and long-term all-cause mortality after multivariable adjustment. Patients in quartiles Q2, Q3, and Q4 had significantly higher mortality than those in Q1, showing a clear trend. Kaplan-Meier analysis confirmed that patients with higher EASIX scores had significantly lower survival. The AUC showed that the performance of EASIX in predicting both short-term and long-term all-cause mortality was comparable to the SOFA and higher than the CHA₂DS₂-VASc score. Stratified analyses indicated that the association remained robust across subgroups, accounting for various underlying conditions and hospital interventions.</p><p><strong>Conclusions: </strong>EASIX is a reliable predictor of both short- and long-term mortality in critically ill patients with AF. Future prospective studies are necessary to confirm its broader applicability in other populations.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"344"},"PeriodicalIF":2.8,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12039053/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143984395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NF-κB-mediated enhancement of H3K27me3 through EZH2: a mechanism to suppress pyocyanin-induced autophagy in macrophages.","authors":"Ji Wang, Min Bian, Simin Liang, Xiaowei Yi, Yu Du","doi":"10.1186/s40001-025-02614-3","DOIUrl":"https://doi.org/10.1186/s40001-025-02614-3","url":null,"abstract":"<p><strong>Background: </strong>Histone modification is a key mechanism of epigenetic regulation. Our previous study demonstrated that histone H3 acetylation at lysine 27 (H3K27ac) promotes pyocyanin (PYO)-induced autophagy in macrophages. However, the regulatory role of H3K27 trimethylation (H3K27me3) in this process remains unclear.</p><p><strong>Methods: </strong>THP-1 macrophages were treated with PYO, and autophagy was assessed by evaluating LC3B II expression and autophagosome formation. The expression of EZH2 and JMJD3 was analyzed to identify the key enzyme responsible for regulating H3K27me3. Nuclear-cytoplasmic fractionation and co-immunoprecipitation were performed to determine the distribution of NF-κB and its interaction with H3K27me3. To explore the role of H3K27me3 in PYO-induced autophagy, cells were co-treated with PYO and EZH2 inhibitors (EI1 or CPI-169), and the transcription of ULK1, BECN1, and MAP1LC3B was analyzed using ChIP-qPCR. Similarly, to assess the role of NF-κB, cells were co-treated with PYO and the NF-κB nuclear translocation inhibitor curcumin, followed by ChIP-qPCR analysis. Finally, the reciprocal transcriptional regulation between NF-κB and H3K27me3 was further investigated.</p><p><strong>Results: </strong>PYO increases LC3B II expression and autophagosome formation in THP-1 macrophages. It also elevates H3K27me3 levels by upregulating EZH2 expression, while JMJD3 remains unchanged. Co-treatment with EZH2 inhibitors reduces H3K27me3 levels, leading to increased LC3B II expression and enhanced autophagosome formation. ChIP-qPCR analysis shows that H3K27me3 enrichment at the ULK1 and MAP1LC3B promoters correlates with reduced transcription, whereas BECN1 remains unaffected. PYO promotes nuclear translocation of NF-κB and enhances its interaction with H3K27me3. ChIP-qPCR further reveals that NF-κB represses the transcription of ULK1 and MAP1LC3B and upregulates EZH2 transcription, which contributes to increased H3K27me3 levels and further suppression of autophagy-related gene expression.</p><p><strong>Conclusions: </strong>The NF-κB/EZH2/H3K27me3 axis plays a pivotal role in suppressing PYO-induced autophagy in macrophages by repressing the transcription of ULK1 and MAP1LC3B.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"346"},"PeriodicalIF":2.8,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12038963/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143976548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neoadjuvant immunochemotherapy for locally advanced esophageal squamous cell carcinoma in real-world practice: an analysis of the clinical outcomes and long-term survival, and the feasibility of using major pathological response as a surrogate endpoint.","authors":"Jiacong Liu, Ziheng Wu, Shihong Zhou, Wang Lv, Yiqing Wang, Pinghui Xia, Linhai Zhu, Jian Hu","doi":"10.1186/s40001-025-02599-z","DOIUrl":"https://doi.org/10.1186/s40001-025-02599-z","url":null,"abstract":"<p><strong>Background: </strong>Neoadjuvant immunochemotherapy is expected to become the standard treatment mode for locally advanced esophageal squamous cell carcinoma (ESCC). This study aims to analyze the clinical outcomes and long-term survival of neoadjuvant immunochemotherapy for locally advanced ESCC, and explore the feasibility of using major pathological response (MPR) as a surrogate endpoint.</p><p><strong>Methods: </strong>This real-world retrospective study consecutively included eligible patients with stage II-IVA locally advanced ESCC who received neoadjuvant immunochemotherapy and surgery between 2019 and 2022 at the Department of Thoracic Surgery, the First Affiliated Hospital, Zhejiang University School of Medicine.</p><p><strong>Results: </strong>This study collected a total of 166 patients, and ultimately included 126 patients after screening. The objective response rate (ORR) was 69.8% (88/126). The incidence of grade 3-4 adverse events (AEs) was 13.5% (17/126). MPR was observed in 49 (38.9%) patients, and 24 (19.0%) patients achieved a complete pathological response (pCR). The median progression-free survival (PFS) was 31.7 months and the 3-year PFS rate was 56.3%. The median overall survival (OS) was not reached and the 3-year OS rate was 70.6%. The median PFS of the non-MPR group was 25.0 months, with the MPR group not achieved (hazard ratio [HR], 2.503; 95% CI 1.359-4.610; P = 0.0022). The median OS in the non-MPR group was 31.7 months and not reached in the MPR group (HR, 3.607; 95% CI 1.576-8.254; P = 0.0012). MPR is an independent prognostic factor affecting OS (HR, 2.522; 95% CI 1.018-6.401; P = 0.046).</p><p><strong>Conclusions: </strong>Neoadjuvant immunochemotherapy is safe and effective for locally advanced ESCC, and can result in certain survival benefits. MPR can serve as a surrogate endpoint for predicting long-term OS.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"342"},"PeriodicalIF":2.8,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12038973/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143976013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intelligent predictive risk assessment and management of sarcopenia in chronic disease patients using machine learning and a web-based tool.","authors":"Ke Rong, Gu Li Jiang Yi Ke Ran, Changgui Zhou, Xinglin Yi","doi":"10.1186/s40001-025-02606-3","DOIUrl":"https://doi.org/10.1186/s40001-025-02606-3","url":null,"abstract":"<p><strong>Background: </strong>Individuals with chronic diseases are at higher risk of sarcopenia, and precise prediction is essential for its prevention. This study aims to develop a risk scoring model using longitudinal data to predict the probability of sarcopenia in this population over next 3-5 years, thereby enabling early warning and intervention.</p><p><strong>Methods: </strong>Using data from a nationwide survey initiated in 2011, we selected patient data records from wave 1 (2011-2012) and follow-up data from wave 3 (2015-2016) as the study cohort. Retrospective data collection included demographic information, health conditions, and biochemical markers. After excluding records with missing values, a total of 2891 adults with chronic conditions were enrolled. Sarcopenia was assessed based on the Asian Working Group for Sarcopenia (AWGS) 2019 guidelines. A generalized linear mixed model (GLMM) with random effects and diverse machine learning models were utilized to explore feature contributions to sarcopenia risk. The Recursive Feature Elimination (RFE) algorithm was employed to optimize the full Multilayer Perceptron (MLP) model and develop an online application tool.</p><p><strong>Results: </strong>Among total population, 580 (20.1%) individuals were diagnosed with sarcopenia in wave 1 (2011-2012), and 638 (22.1%) were diagnosed in wave 3 (2015-2016), while 2165 (74.9%) individuals were not diagnosed with sarcopenia across the study period. MLP model, performed better than other three classic machine learning models, demonstrated a ROC AUC of 0.912, a PR AUC of 0.401, a sensitivity of 0.875, a specificity of 0.844, a Kappa value of 0.376, and an F1 score of 0.44. According to MLP model-based SHapley Additive exPlanations (SHAP) scoring, weight, age, BMI, height, total cholesterol, PEF, and gender were identified as the most important features of chronic disease individuals for sarcopenia. Using the RFE algorithm, we selected six key variables-weight, age, BMI, height, total cholesterol, and gender-achieving an ROC AUC of about 0.9 for the online application tool.</p><p><strong>Conclusion: </strong>We developed an MLP machine learning model that incorporates only six easily accessible variables, enabling the prediction of sarcopenia risk in individuals with chronic diseases. Additionally, we created a practical online application tool to assist in decision-making and streamline clinical assessments.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"345"},"PeriodicalIF":2.8,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12039279/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143991582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Opsin3 regulates cell proliferation, migration, and apoptosis in lung adenocarcinoma via GPX3 pathway.","authors":"Xiaojia Li, Yu Wang, Yan Liu, Qingwei Meng","doi":"10.1186/s40001-025-02581-9","DOIUrl":"https://doi.org/10.1186/s40001-025-02581-9","url":null,"abstract":"<p><p>Despite recent progress in understanding lung adenocarcinoma (LUAD) and the emergence of new therapeutic strategies, LUAD continues to be one of the deadliest lung cancer types, with a five-year survival rate of under 5%. Opsin3 (OPN3), a member of the G protein-coupled receptor superfamily, has been linked to various cancer-related processes, including tumor progression and therapy resistance. However, its specific role in LUAD remains insufficiently investigated. This study aimed to explore OPN3's regulatory functions in LUAD and evaluate its potential as a therapeutic target. OPN3 expression in LUAD cells was assessed using quantitative PCR, Western blotting, and immunohistochemistry. The effects of OPN3 on cell migration and invasion were evaluated through wound healing and transwell assays. Additionally, the influence of OPN3 on cell cycle progression and signaling pathways in vivo-critical for cellular responses to external stimuli-was examined. Pathway enrichment analysis revealed significant disruption of genes associated with glutathione metabolism. Notably, a strong correlation between OPN3 expression and the regulation of Glutathione Peroxidase 3 (GPX3), a key enzyme in this metabolic pathway, was identified. Our results demonstrate that OPN3 is markedly overexpressed in LUAD tissues relative to normal lung tissues. Silencing OPN3 via siRNA significantly diminished the malignant features of LUAD cells, including proliferation, migration, and invasion. In contrast, OPN3 overexpression enhanced these malignant characteristics, indicating its involvement in tumor progression. Moreover, an inverse relationship between OPN3 expression and GPX3 levels was observed, suggesting that OPN3 may drive LUAD progression through the GPX3 pathway. This study offers new insights into the function of OPN3 in LUAD and suggests that targeting the OPN3-GPX3 axis could provide a promising therapeutic strategy for LUAD patients.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"343"},"PeriodicalIF":2.8,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12038953/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143991781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Frailty in older adults patients: a prospective observational cohort study on subtype identification.","authors":"Zhikai Yang, Chen Ji, Ting Wang, Wei He, Yuhao Wan, Min Zeng, Di Guo, Lingling Cui, Hua Wang","doi":"10.1186/s40001-025-02450-5","DOIUrl":"10.1186/s40001-025-02450-5","url":null,"abstract":"<p><strong>Background: </strong>While the FRAIL scale has been used in primary care, cluster analysis on frail patients in a hospital setting has not been performed.</p><p><strong>Objectives: </strong>To identify potential subtypes of frail patients, and develop a simple, clinically applicable model for improved patient management.</p><p><strong>Methods: </strong>The study included 214 frail patients aged 65 and above who were hospitalized in a hospital in Beijing from September 2018 to April 2019. This study applied the K-means clustering algorithm to analyze 27 variables, determining the optimal cluster number using the Elbow method and Silhouette coefficient. Key variables for predictive modeling were identified through LASSO (least absolute shrinkage and selection operator) regression, SVM-RFE (support vector machine-recursive feature elimination), and random forest techniques. A logistic regression model was then developed to predict patient subtypes, aimed at enhancing clinical identification and management of frailty subtypes.</p><p><strong>Results: </strong>Clustering analysis distinguished two unique subgroups among the frail patients, revealing significant disparities in clinical characteristics and survival outcomes. One-year survival rates for Class 1 and Class 2 were 62.51% and 47.51%, respectively. The logistic regression model exhibited robust predictive capability, with an AUC (Area under curve) of 0.88. Validation through 1000 bootstrap resamples confirmed the model's reliability, with an average AUC of 0.8707 and a 95% CI (Confidence intervals) of 0.8572 to 0.8792.</p><p><strong>Conclusions: </strong>This study identifies two frailty subtypes in a hospital setting using unsupervised machine learning, demonstrating significant differences in survival outcomes. Clinical Trial registration ChiCTR1800017204; date of reqistration: 07/18/2018.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"336"},"PeriodicalIF":2.8,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12036271/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143987086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From infection to infertility: a review of the role of human papillomavirus-induced oxidative stress on reproductive health and infertility.","authors":"Omid Salahi Ardekani, Arash Letafati, Sepehr Ebrahimi Dehkordi, Ali Vasheghani Farahani, Mahshid Bahari, Bahar Mahdavi, Negar Ariamand, Mahdie Taghvaei, Moein Kohkalani, Angila Ataei Pirkooh, Seyed Mohammad Jazayeri, Luciano Saso","doi":"10.1186/s40001-025-02605-4","DOIUrl":"https://doi.org/10.1186/s40001-025-02605-4","url":null,"abstract":"<p><p>Infertility has emerged as a significant global health concern, affecting nearby 8-12% of couples in reproductive age worldwide. Increasing evidence suggests a potential link between human papillomavirus (HPV) and infertility in both men and women. Some research indicate that HPV can infect various components of semen, potentially affecting sperm quality by decreasing motility, viability, and increasing DNA fragmentation, all of which may contribute to male infertility. The virus can attach to the equatorial region of the sperm head, enabling infected sperm to transmit the virus to the oocyte or placenta. Consequently, HPV potentially induces apoptosis in trophoblastic cells and disrupts their adhesion to endometrial cells, which raises the risk of miscarriage. HPV may also affect ovarian reserve by causing chronic inflammation, which can impair granulosa cell function and lower serum anti-Müllerian hormone (AMH) levels. Besides, HPV-related immune responses also contribute to infertility by producing anti-sperm antibodies (ASAs), which cause sperm clumping, reduce motility through cervical mucus, activate the complement system that damages sperm in the female reproductive tract and interfere with sperm-egg interactions. Moreover, HPV infection has been linked to reduced success rates in assisted reproductive technologies (ART), potentially disrupting critical processes such as the acrosome reaction, sperm-oocyte interaction, and fusion. One potential mechanism through which HPV contributes to infertility is oxidative stress (OS). Triggered OS can negatively impact sperm quality and cause damage to the female reproductive system, ultimately contributing to infertility. Despite these associations, the precise mechanisms and the strength of the relationship remain uncertain. Thus, this review seeks to investigate the potential impact of HPV on infertility, particularly its effects on the reproductive system through OS. A clearer understanding of these processes could inform future health strategies for addressing HPV-related infertility.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"339"},"PeriodicalIF":2.8,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12036311/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143993357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biopsy and interventional therapy of subglottic lesions with flexible bronchoscope under protection of endotracheal intubation.","authors":"Huihui Lin, Tingfen Ji, Hao Zheng, Wenjiang Ma, Shengwen Song, Yueying Zheng, Danjuan Yu, Lijing Ye, Hequan Li","doi":"10.1186/s40001-025-02615-2","DOIUrl":"https://doi.org/10.1186/s40001-025-02615-2","url":null,"abstract":"<p><p>Subglottic lesions represent a complex and challenging clinical entity, often associated with high procedural risks due to their anatomical location and vascularity. While a laryngeal mask airway (LMA) is commonly employed for airway management in such cases, it has notable limitations, particularly in scenarios involving significant bleeding or the need for extended intervention. This article presents an alternative and effective approach for the biopsy and interventional treatment of subglottic lesions using a flexible bronchoscope under the protection of an endotracheal tube (ETT). By inserting the bronchoscope through the space between the ETT and the tracheal wall, various procedures-including biopsy, argon plasma coagulation (APC), and CO₂ cryotherapy-can be performed with relative safety. This method provides a feasible option for airway protection, bleeding control, and lesion management, offering new possibilities in clinical practice.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"337"},"PeriodicalIF":2.8,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12036187/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143988177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proteomics and lipidomics of human umbilical cord mesenchymal stem cells exposed to ionizing radiation.","authors":"Dongmei Han, Li Ding, Xiaoli Zheng, Sheng Li, Hongmin Yan, Jing Liu, Hengxiang Wang","doi":"10.1186/s40001-025-02578-4","DOIUrl":"https://doi.org/10.1186/s40001-025-02578-4","url":null,"abstract":"<p><strong>Objectives: </strong>Mesenchymal stem cell (MSC)-based therapies exhibit beneficial effects on various forms of tissue damage, including ionizing radiation-induced lesions. However, whether ionizing radiation affects the functions of human umbilical cord mesenchymal stem cells (hucMSCs) remains unclear. This study aimed to investigate the effect and possible mechanisms of ionizing radiation on the proliferation and differentiation of hucMSCs.</p><p><strong>Methods: </strong>The hucMSCs were divided into the 1 Gy group (exposure to a single dose (1 Gy) of X-ray radiation (1 Gy/min) for 14 days) and control (without radiation treatment) group. The proliferation, apoptosis, and adipogenic and osteogenic differentiation abilities of hucMSCs in the two groups were evaluated. Moreover, the lipidomics and proteomics analyses were conducted to explore crucial lipids and proteins by which ionizing radiation affected the functions of hucMSCs. In addition, the effects of BYSL on radiation-treated hucMSCs were explore, as well as the involved potential mechanisms.</p><p><strong>Results: </strong>X-ray radiation treatment inhibited proliferation, promoted apoptosis, and decreased adipogenic and osteogenic differentiation abilities of hucMSCs. Key lipids, such as triglyceride (TG) and phosphatidylcholine (PC), and hub proteins (BYSL, MRTO4, and RRP9) exhibited significant differences between the 1 Gy group and control group. Moreover, BYSL, MRTO4, and RRP9 were significantly correlated with TG and PC. BYSL overexpression evidently promoted the cell proliferation, adipogenic and osteogenic differentiation abilities of radiation-treated hucMSCs, as well as the protein expression levels of p-GSK-3β/GSK-3β and β-catenin, while suppressed cell apoptosis. However, the GSK-3β inhibitor (1-Az) treatment reversed the protein expression levels of p-GSK-3β/GSK-3β, β-catenin and BYSL, as well as the cell proliferation, apoptosis, adipogenic and osteogenic differentiation abilities of radiation-treated hucMSCs.</p><p><strong>Conclusions: </strong>Our findings reveal that the proliferation and differentiation of hucMSCs are suppressed by radiation, which may be associated with the changes of key lipids (TG and PC) and proteins (BYSL, MRTO4, and RRP9). Furthermore, BYSL promotes adipogenic and osteogenic differentiation abilities of radiation-treated hucMSCs via GSK-3β/β-catenin pathway. These findings help explain the response of hucMSCs to radiation and have clinical implications for improving the outcomes of MSC-based therapies after radiotherapy.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"340"},"PeriodicalIF":2.8,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12036141/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143973883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of T cells in the progression of dry eye disease using single-cell RNA sequencing in mice.","authors":"Zhizhi Zhang, Liwei Zhang, Baihua Chen","doi":"10.1186/s40001-025-02607-2","DOIUrl":"https://doi.org/10.1186/s40001-025-02607-2","url":null,"abstract":"<p><strong>Background: </strong>Increasing evidence indicated that T cells have significant effects in dry eye disease (DED). However, the regulatory role of T cells in DED remains unclear.</p><p><strong>Methods: </strong>In this study, we examined immune responses throughout the progression in murine DED model. Using cytometry by time-of-flight (CyTOF) and single-cell RNA sequencing (scRNA-seq), we observed dynamic alterations in the proportions of immune cell landscape. Pseudotime trajectory and cell-cell communication analyses further illustrated T-cell differentiation and interaction networks.</p><p><strong>Results: </strong>CD4⁺ and CD8⁺ T cells exhibited an initial decline on Day 3 (D3) and followed by a recovery on Day 7 (D7). Single-cell transcriptomics provided insights into 15 distinct subsets of T cells with heterogeneous functional states. Pseudotime trajectory analysis demonstrated coordinated differentiation patterns of CD4⁺ and CD8⁺ T cells, indicating their collaborative involvement in the inflammatory process.</p><p><strong>Conclusions: </strong>Our results clarify the dynamics of the adaptive immune response in DED and indicate that targeting T cells may serve as a promising immune-modulatory approach in the treatment of DED model.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"338"},"PeriodicalIF":2.8,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12036131/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143989196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}