European Journal of Medical Research最新文献

{"title":"Saliva as a potential and non-invasive approach to identify upregulated genes associated with comorbidities of T1DM: a brief report.","authors":"Bashair M Mussa, Ankita Srivastava, Reeja Rajan, Thenmozhi Venkatachalam, Abeer Al-Habshi, Elamin Abdelgadir, Alaaeldin Bashier, Fatheya Al Awadi, Khadija Hafidh, Rifat Hamoudi, Salah Abusnana","doi":"10.1186/s40001-025-03105-1","DOIUrl":"10.1186/s40001-025-03105-1","url":null,"abstract":"<p><strong>Background: </strong>One of the main challenges in Type 1 diabetes mellitus (T1DM) research is the sample size of the participants and the invasive process of collecting an adequate number of blood samples from young patients with T1DM. Therefore, it is of great interest to investigate the possibility of using saliva as a non-invasive tool to investigate the genetic factors that are associated with T1DM comorbidities. The present study aims to identify differentially expressed genes (DEGs) in saliva samples of T1DM patients with various comorbidities using transcriptomic profiling.</p><p><strong>Methods: </strong>A total of 56 participants were recruited from the University Hospital Sharjah, Dubai Hospital and Rashid Hospital, United Arab Emirates. Participants were divided into various groups: Control Group: healthy Emirati (n = 13), Group 1: patients with T1DM without any comorbidities (G1; n = 14), Group 2: patients with T1DM and hyperlipidemia (G2; n = 10), Group 3: patients with T1DM and neuropathy (G3; n = 5), Group 4: patients with T1DM and ketoacidosis (G4; n = 6), Group 5: patients with T1DM and hypothyroidism (G5; n = 6) and Group 6: patients with T1DM and polycystic ovary syndrome (PCOS, G6; n = 5). Saliva samples and blood were collected from all participants and RNA was extracted for transcriptomic analysis.</p><p><strong>Results: </strong>The transcriptomic analysis of saliva samples of T1DM patients showed several DEGs that were associated with T1DM comorbidities. Interestingly, the number of upregulated genes in blood (n = 350) and saliva (n = 353) was comparable in the PCOS group (G6). In addition, the number of DEGs was more than double in saliva (n = 270) compared to blood (n = 118) samples in the ketoacidosis group (G4). Ten common upregulated genes between saliva and blood were identified in T1DM patients with hyperlipidemia (G2): LYPD5, TCF15, PRKY, CYHR1, CCDC173, CHRNA5, MIAT, HBA2, RAD54L, and TSHZ3. In addition, two common upregulated genes were identified in the neuropathy group (G3): KCTD19, FAM209B. Moreover, the following upregulated genes were observed in ketoacidosis (G4), hypothyroidism (G5) and PCOS (G6): (NCKAP5, ADAD2, C20orf144), (ZFY, KCTD19, P2RY14, RPS60) and (C4orf19, RSAD2, MEGF10, CMPK2, CHRNA5, TSHZ3, CCDC77, CLEC12A, RTP4), respectively.</p><p><strong>Conclusions: </strong>The present findings demonstrated that the number of upregulated genes can be comparable between saliva and blood of T1DM patients with comorbidities, and in some groups, the number of upregulated genes was higher in the saliva compared to blood, emphasizing that saliva can be a potential non-invasive tool to identify DEGs in T1DM comorbidities.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"955"},"PeriodicalIF":3.4,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12512297/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145274267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between sleep duration and estimated glomerular filtration rate in Chinese patients with diabetes: evidence from a cross-sectional study.","authors":"Cishuang Fu, Zhiming Deng","doi":"10.1186/s40001-025-03240-9","DOIUrl":"10.1186/s40001-025-03240-9","url":null,"abstract":"<p><strong>Background: </strong>Previous epidemiological studies have demonstrated substantial associations between sleep duration and diabetes, vascular complications of diabetes, and cardiovascular disease in diabetic patients. However, few studies have so far explored the effect of sleep time on estimated glomerular filtration rate (eGFR) in patients with diabetes. This study was performed for the purpose of exploring the relationship between sleep duration and eGFR in diabetic patients.</p><p><strong>Methods: </strong>This study analyzed 1389 patients with diabetes from the National Metallic Management Center in China. This cross-sectional study assessed sleep duration and eGFR in patients with diabetes. Sleep duration at night was categorized into four groups: very short (< 6 h), short (6-6.9 h), optimal (7-8 h), and long (> 8 h). The association of sleep duration with eGFR was analyzed using univariate linear regression, and generalized additive models were applied to assess the non-linear relationship between sleep duration and eGFR.</p><p><strong>Results: </strong>Compared to optimal sleep duration (7-8 h), both long sleep duration (> 8 h) and very short sleep duration (< 6 h) were associated with level of eGFR (β = -3.63, 95%CI: - 5.54 to - 1.71, P = 0.0002 and β = - 4.79, 95%CI: - 8.55 to - 1.03, P = 0.0126, respectively). The smooth curve showed that the relationship between sleep duration and eGFR level was non-linear after adjusting for potential confounders. Through the use of a two-piecewise linear regression model, we calculated the inflection point was 7 h.</p><p><strong>Conclusions: </strong>Both very short and long sleep durations were associated with low eGFR in diabetics. There is a U-shaped relationship between sleep duration and eGFR levels.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"953"},"PeriodicalIF":3.4,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12512362/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145274118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The miR-3164/PAD4 axis regulates NETosis to prevent airway inflammation and remodeling through the TLR2/NF-κB signaling pathway.","authors":"Liang He, Ruixue Qiang, Weiqun Li","doi":"10.1186/s40001-025-03175-1","DOIUrl":"10.1186/s40001-025-03175-1","url":null,"abstract":"<p><strong>Background: </strong>Alleviating airway inflammation and reversing airway remodeling are critical therapeutic objectives in asthma treatment. This study aimed to investigate the role of miR-3164 in regulating PAD4-mediated NETosis and to elucidate the underlying mechanisms through which it attenuates airway inflammation and reverses remodeling.</p><p><strong>Methods: </strong>The effects of miR-3164 on PAD4 expression and neutrophil extracellular trap (NET) formation were evaluated via dual-luciferase reporter assays, Western blotting, quantitative real-time PCR (qRT‒PCR), and flow cytometry. The influence of neutrophils treated with miR-3164 mimics on the proliferation and migration of mouse airway smooth muscle cells (ASMCs) was assessed via MTT and Transwell assays.</p><p><strong>Results: </strong>The dual-luciferase reporter assay confirmed a targeting relationship between miR-3164 and PAD4. Treatment with miR-3164 mimics suppressed PAD4 expression in neutrophils and significantly altered the levels of myeloperoxidase (MPO) and neutrophil elastase (NE) within NETs. These effects confer the ability of miR-3164 mimics to markedly downregulate the expression of inflammation- and airway remodeling-related biomarkers, including TLR2, IκBα, NF-κB, calponin, α-SMA, and E-cadherin, in airway smooth muscle cells (ASMCs). Furthermore, NETs treated with miR-3164 mimics significantly attenuated the viability and migration capacity of ASMCs exposed to LPS and ATP.</p><p><strong>Conclusions: </strong>Our findings demonstrate that modulating NETosis represents a potential therapeutic strategy to attenuate airway inflammatory responses and remodeling via the TLR2/NF-κB signaling pathway, thereby offering a novel target for the treatment of asthma.</p><p><strong>Clinical trial number: </strong>Not applicable.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"947"},"PeriodicalIF":3.4,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12512812/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145257869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and validation of feature genes of acute myocardial infarction based on ferroptosis-related genes.","authors":"Yuting Zhou, Yehong Liu, Baida Xu, Tianhui Jin, Ting Ye, Wentao Su, Chengsi Li, Tingting Kang, Haoran Xie, Gangjun Zong","doi":"10.1186/s40001-025-03216-9","DOIUrl":"10.1186/s40001-025-03216-9","url":null,"abstract":"<p><strong>Background: </strong>Acute myocardial infarction (AMI) represents one of the most severe complications of coronary artery disease (CAD) and is a leading cause of mortality from noncommunicable diseases globally. In recent years, with the deepening of research on ferroptosis, the role of this cell death mode accompanied by iron accumulation and lipid peroxidation in myocardial infarction has been increasingly confirmed, which eventually leads to cell oxidative death. However, prognosis mechanism, and participation degree of ferroptosis in the occurrence of AMI still need to be further explored.</p><p><strong>Methods: </strong>The gene expression data sets (GSE97320, GSE60993) of AMI patients and the control group were obtained from the Gene Expression Omnibus (GEO) data sets. Screening for ferroptosis-related differentially expressed genes (FDEGs). In addition, the biological functions of these FDEGs were analyzed by Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. Least absolute shrinkage and selection operator (LASSO) and support vector machine-recursive feature elimination (SVM-RFE) were used to further check FDEGs to construct the prognostication model. ROC curves were used to validate the model's accuracy. GSE48060 and GSE59867 were set as a validation set to verify the model. Finally, the peripheral blood of AMI and control samples was collected for qRT-PCR to examine the differential expression situation of FDEGs.</p><p><strong>Results: </strong>The CYB5R1, TSC1, LAMP2, PARK7, and MGST1 were identified as the most characteristic FDEGs, and the diagnostic model composed of them has excellent diagnostic ability for AMI.</p><p><strong>Conclusions: </strong>The identification of FDEGs in AMI by machine learning is a reliable research method that can provide further ideas for the study of ferroptosis in AMI.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"948"},"PeriodicalIF":3.4,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12512630/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145257849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HMGB1 regulates autophagy via TLR4/NF-κB signaling pathway in myocardial injury induced by coronary microembolization.","authors":"Tingsheng Yang, Xiaodan Wu, Xiantao Wang","doi":"10.1186/s40001-025-03220-z","DOIUrl":"10.1186/s40001-025-03220-z","url":null,"abstract":"<p><strong>Background: </strong>Coronary microembolization (CME) is a common and refractory complication of coronary atherosclerotic plaque spontaneous rupture or reperfusion treatment in patients with coronary heart disease, which is closely related to the patient's near-term and long-term prognosis. The specific molecular mechanism of CME leading to myocardial injury is not yet fully understood. This study aims to elucidate the regulatory function of high-mobility group protein box 1 (HMGB1) within the TLR4/NF-κB signaling pathway, and explore the role of autophagy mediated by this signaling pathway in CME-induced myocardial injury.</p><p><strong>Methods: </strong>A rat model of CME was established by left ventricle injection of microspheres. HMGB1 inhibitor (glycyrrhizin) or autophagy inhibitor (3-methyladenine) was pretreated before CME modeling. Following the animal modeling of CME, we examined alterations in the expression and localization of proteins associated with signaling pathways. Additionally, we monitored the autophagic activity within the myocardium and assessed cardiac function, histological changes in myocardium, and biomarkers indicative of myocardial injury present in serum samples.</p><p><strong>Results: </strong>In the CME model group, there was a significant upregulation of HMGB1 expression and its migration into the cytoplasm. Concurrently, TLR4 and NF-κB expression levels were elevated, while autophagic activity was diminished. In contrast, when compared to the model group, the glycyrrhizin acid pretreatment group exhibited notable improvements in cardiac dysfunction (LVEF increased by 18.50 ± 1.13%) and myocardial injury (microinfarct size reduced by 19.94 ± 1.07%, P < 0.001). However, this cardioprotective effect was nullified upon pretreatment with the autophagy inhibitor 3-MA.</p><p><strong>Conclusion: </strong>HMGB1 regulates autophagy through TLR4/NF-κB signaling pathway in CME rat model of myocardial injury induced by coronary microembolization.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"943"},"PeriodicalIF":3.4,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12512573/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145257860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular interplay between TXNIP and GLUT9 underlies uric acid transport dysregulation in vitro under hyperuricemic stress.","authors":"Yuqiu Zhu, Bei Zhang, Yi He, Manxi Du, Wujin Chen, Xiaoyu Chen, Qingqing Yang, Mayina Kahaer, Yuping Sun","doi":"10.1186/s40001-025-03209-8","DOIUrl":"10.1186/s40001-025-03209-8","url":null,"abstract":"<p><strong>Background: </strong>This study aimed to investigate the role of Thioredoxin Interacting Protein (TXNIP) in hyperuricemia, specifically examining its influence on uric acid (UA) metabolism and the associated processes.</p><p><strong>Methods: </strong>A hyperuricemia cellular model was created using human renal tubular epithelial cells (HK-2). The DCFH-DA probe was utilized to quantify the quantities of reactive oxygen species (ROS) within cells. Cell lines exhibiting TXNIP overexpression and knockdown were established. The impact of TXNIP overexpression on UA metabolism in HK-2 cells was examined using integrated metabolomic and transcriptome analysis. Real-time fluorescence quantitative PCR (RT-qPCR) and Western blot were employed to assess the expression levels of TXNIP and the UA transporters GLUT9, ABCG2, URAT1, and OAT3. The connection between TXNIP and GLUT9 was investigated by the Co-immunoprecipitation. Additionally, immunofluorescence labeling was utilized to examine the subcellular localization of TXNIP and GLUT9.</p><p><strong>Results: </strong>A high UA environment stimulated ROS generation and markedly elevated TXNIP expression. Research utilizing metabolomics and transcriptomics suggests that TXNIP may modify the UA metabolic pathway in HK-2 cells, hence affecting UA stability. Conversely, TXNIP overexpression enhanced net UA uptake in HK-2 cells by elevating GLUT9 expression, but TXNIP knockdown yielded the contrary effect. Immunofluorescence labeling demonstrated the co-localization of TXNIP and GLUT9 in HK-2 cells. CO-IP studies demonstrated that TXNIP interacted with GLUT9.</p><p><strong>Conclusion: </strong>TXNIP may influence UA reabsorption in hyperuricemia via ROS-induced upregulation and subsequent enhancement of its expression through interaction with the UA transporter protein, GLUT9, hence impacting the progression of hyperuricemia.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"942"},"PeriodicalIF":3.4,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12512431/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145257853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The association between systemic immune-inflammatory index and cardiac biomarkers among US population: a cross-sectional study.","authors":"Xinmeng Liu, Shihao Jin, Mei Ma","doi":"10.1186/s40001-025-03143-9","DOIUrl":"10.1186/s40001-025-03143-9","url":null,"abstract":"<p><strong>Background: </strong>The systemic immune-inflammatory index (SII), calculated from platelet, neutrophil, and lymphocyte counts, is a novel marker of inflammation potentially relevant to cardiac disease. While cardiac biomarkers are used clinically, the relationship between SII and these markers in the general population is unclear.</p><p><strong>Methods: </strong>We analyzed data from 3206 US adults (aged 20-85 years) in the 1999-2004 National Health and Nutrition Examination Survey (NHANES). Multivariable linear regression models, adjusted for demographics, comorbidities, and lifestyle factors, assessed cross-sectional associations of SII with cardiac biomarkers. Nonlinearity was examined using restricted cubic splines. Subgroup analyses explored effect modification.</p><p><strong>Results: </strong>SII showed a consistent positive association with C-reactive protein (CRP) levels across all models (All P < 0.001). In contrast, the positive association of SII with NT-pro BNP observed in the unadjusted model (β = 0.20; 95% CI 0.11, 0.29; P < 0.001) was attenuated and became non-significant after full adjustment in the general population. Subgroup analyses showed significantly positive associations between SII and NT-pro BNP specifically in individuals aged > 55 years (β = 0.17; 95% CI 0.014, 0.33), and in those with myocardial infarction (MI) (β = 0.82; 95% CI 0.22, 1.43) or angina pectoris (AP) (β = 0.73; 95% CI 0.19, 1.47). The robust SII-CRP association was confirmed in subgroups. Dose-response relationships were supported by spline analyses.</p><p><strong>Conclusions: </strong>SII is persistently associated with CRP levels, supporting its role as an inflammatory indicator. Significant associations between SII and NT-pro BNP were observed only in specific high-risk subgroups (aged > 55 years, MI or AP patients), but not in the fully adjusted general population. These findings suggest SII may have particular relevance in specific cardiovascular contexts.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"949"},"PeriodicalIF":3.4,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12512276/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145257858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical application of rapid on-site evaluation of technology for the diagnosis of respiratory system tumors: a retrospective study.","authors":"Lingling Hong, Xiaoqiong Yang, Yuqing Chen, Weiwei Shao, Weikai Zeng","doi":"10.1186/s40001-025-03212-z","DOIUrl":"10.1186/s40001-025-03212-z","url":null,"abstract":"<p><strong>Objectives: </strong>This study aimed to investigate the clinical application of rapid on-site evaluation (ROSE) technology in the diagnosis of respiratory system tumors.</p><p><strong>Methods: </strong>A total of 175 consecutive hospitalized patients with a suspected respiratory system tumors between 2019 and 2024 were enrolled (124 from 2019 to 2022, and 20 from 2022 to 2024). Here, 144 cases underwent conventional pathological examination with ROSE assistance, while 31 patients received examination without ROSE. The concordance rate between ROSE and pathological results was compared, along with the timeliness of reports from 2019 to 2022. Using pathological examination results as the \"gold standard,\" the difference in diagnostic positive rates between the groups with and without ROSE was compared.</p><p><strong>Results: </strong>The concordance rate between ROSE and pathological diagnosis was 95.83%. The median time of DQ staining in 2019-2022 was 8.0 min, and the median time of pathological results was 5645.5 min, with statistical difference between the two groups (P < 0.05). The average time of pathological report in 2022-2024 was 1446.5 min, the longest time was 2562.0 min, and the shortest time was 1118.0 min. The concordance rate with pathological examination was 95.83% for the ROSE group and 83.87% for the non-ROSE group. The difference in positive rates between the ROSE group and the non-ROSE group was statistically significant (P < 0.05). Using pathological examination as the \"gold standard,\" the concordance rate for dual-operator ROSE was 95.97%, and for single-operator ROSE was 95.00% (P = 0.845). The average blood loss of the searchable ROSE group (16 cases) and non-ROSE group (27 cases) in 2022-2024 was 4.87 mL, 2.37 mL, and there was no statistically significant difference between the two groups (P > 0.05).</p><p><strong>Conclusions: </strong>ROSE technology demonstrated a higher positive rate, thereby improving the pathological diagnostic yield compared to conventional pathological examination alone. This enables clinicians to obtain preliminary diagnoses earlier, guides sampling site and quantity, possesses considerable clinical value, and warrants promotion.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"950"},"PeriodicalIF":3.4,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12512841/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145257881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Factors affecting etiology and En-DCR outcomes in nasolacrimal duct obstruction: a retrospective cohort study.","authors":"Xinyue Yu, Kerui Wang, Rongxin Chen, Yunming Liu, Meiqing Chen, Xuehua Liu, Shuiling Li, Jing Zhao, Yongqing Shen, Xielan Kuang, Huangxuan Shen, Xuanwei Liang","doi":"10.1186/s40001-025-03162-6","DOIUrl":"10.1186/s40001-025-03162-6","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to characterize the statistical profiles of potential pathogenic factors and identify determinants influencing the success of endoscopic dacryocystorhinostomy (En-DCR) through comprehensive analysis of clinical data from patients with nasolacrimal duct obstruction (NLDO).</p><p><strong>Methods: </strong>We reviewed 1008 patients (1140 eyes) with nasolacrimal duct obstruction (NLDO) who underwent endoscopic dacryocystorhinostomy (En-DCR) between September 2020 and June 2023. Data on demographics, clinical features, previous diagnosis and treatment, preoperative interventions, and the results of NLDO-related examinations were recorded. Postoperative outcomes were assessed by reviewing clinic visit records and telephone follow-up conducted during routine care, with success defined as complete resolution of epiphora and patent irrigation. Multivariable logistic regression was used to identify independent risk factors associated with surgical outcome.</p><p><strong>Results: </strong>Among 1008 patients (1140 eyes), 35.9% had snoring/sleep apnea, 20.9% gastroesophageal reflux disease (GERD), and 19.3% a family history of epiphora. Of 851 females, 80.5% were menopausal, with 26.8% having gynecologic disease history and 11.5% prior gynecologic surgery. En-DCR achieved a 95.6% success rate. Multivariable analysis identified regurgitation on pressure over the lacrimal sac (ROPLAS) sign (OR = 0.25, 95% CI 0.13-0.48, p < 0.001) and minimum lacrimal sac diameter on dacryocystography (OR = 8.71, 95% CI 2.57-29.57, p < 0.001) as independent predictors of outcome.</p><p><strong>Conclusion: </strong>NLDO frequently coexists with comorbidities including paranasal sinusitis/nasal polyps, chronic conjunctivitis, dry eye, GERD, family history of epistaxis, and sleep-disordered breathing (sleep apnea/snoring). Notably, ROPLAS sign and minimum lacrimal sac diameter ≤ 3 mm on dacryocystography independently predict En-DCR outcomes, with the latter demonstrating a particularly strong association (OR = 8.71, p < 0.001). These findings highlight the importance of preoperative assessment of lacrimal sac anatomy and clinical signs in optimizing surgical success.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"946"},"PeriodicalIF":3.4,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12512915/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145257828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ouabain-mediated IGF2BP2-GLS/NOS3 axis affects arginine metabolism in diffuse large B-cell lymphoma.","authors":"Yuxin Hong, Hehua Ma, Zhuoling Zhao, Wei Song, Juan Li","doi":"10.1186/s40001-025-03183-1","DOIUrl":"10.1186/s40001-025-03183-1","url":null,"abstract":"<p><p>Our group has previously demonstrated that ouabain can contribute to the malignant progression of diffuse large B-cell lymphoma (DLBCL) by regulating insulin-like growth factor 2 mRNA binding protein (IGF2BP2) at the genetic level. However, its potential impact on DLBCL metabolism remains unclear. To investigate this, we introduced ouabain or IGF2BP2 knockdown into DLBCL cells and observed that both treatments influenced the arginine metabolic pathway, as revealed by non-targeted metabolomics analysis. We further found significant changes in two genes: nitric oxide synthase 3 (NOS3) and glutaminase (GLS), which were involved in arginine metabolism. And it confirmed that NOS3 and GLS are highly expressed in DLBCL and associated with shorter patient survival. Real-time quantitative PCR (RT-qPCR) indicated that the expression levels of NOS3 and GLS were regulated by IGF2BP2. Cell proliferation was inhibited after interference with GLS and NOS3. Cell cycle and apoptosis experiments revealed that interference with GLS and NOS3 can delay cell progression and promote cell apoptosis. In conclusion, our study reveals that ouabain can interfere with arginine metabolism via the IGF2BP2-GLS/NOS3 axis, contributing to the progression of DLBCL. This provides novel targeted therapeutic strategies and potential targets for DLBCL treatment.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"945"},"PeriodicalIF":3.4,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12512809/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145257851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}