European Journal of Medical Research最新文献

{"title":"Association of systemic inflammatory biomarkers with ocular disease: a large population-based cross-sectional study.","authors":"Xue Wang, Haitao Jiang, Can Zhang","doi":"10.1186/s40001-025-02473-y","DOIUrl":"10.1186/s40001-025-02473-y","url":null,"abstract":"<p><strong>Background: </strong>The aim of this study was to explore the association of systemic inflammatory biomarkers (systemic immune-inflammation (SII) index and systemic inflammatory response index (SIRI)) with the prevalence of ocular disease in the general population of the United States (U.S.).</p><p><strong>Methods: </strong>We conducted a cross-sectional study of subjects in the National Health and Nutrition Examination Survey 2005-2008 years. For the analysis of the association of SII index, and SIRI with the prevalence of ocular disease (glaucoma, cataract, age-related macular degeneration (ARMD), and diabetic retinopathy), the restricted cubic spline (RCS) plot, multivariable logistic regression models, and subgroup analysis were performed.</p><p><strong>Results: </strong>There was a total of 5377 individuals. As shown by the RCS plot, SII index and SIRI were linked with ARMD risk in a U-shaped pattern. Additionally, the SII index and SIRI were linearly positive with glaucoma and cataract. Finally, the risk of diabetic retinopathy was associated with the L-shaped and N-shaped curves of the SII index and SIRI, respectively.</p><p><strong>Conclusions: </strong>Two new systemic inflammatory biomarkers, SII index and SIRI, are closely related to the risk of eye disease. There are different associations between SII index and different ocular diseases. This should raise more concerns and lead to better prevention strategies for systemic inflammation.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"206"},"PeriodicalIF":2.8,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11938706/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143718177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reducing stroke-associated pneumonia through pulmonary rehabilitation in moderate-to-severe acute ischemic stroke.","authors":"QingSha Zhang, HongKun Liu, Jie Sun, HongGe Shi","doi":"10.1186/s40001-025-02451-4","DOIUrl":"10.1186/s40001-025-02451-4","url":null,"abstract":"<p><strong>Objectives: </strong>This study investigated the effect of a comprehensive pulmonary rehabilitation (CPR) program on stroke-associated pneumonia (SAP) in patients with moderate-to-severe acute ischemic stroke (AIS) after thrombolysis.</p><p><strong>Methods: </strong>This study was a prospective randomized controlled intervention study. Eighty patients with moderate-to-severe AIS were divided into the conventional rehabilitation (CR) and CPR groups. Demographic and general clinical data were collected. Patients were evaluated by the Fatigue Severity Scale (FSS), Fugl-Meyer Assessment (FMA), and Fugl-Meyer balance (FMB). The incidence of pneumonia in the acute phase and the treatment efficacy were compared.</p><p><strong>Results: </strong>FSS scores at T1 and T2 (2 weeks and 4 weeks after treatment), FMA scores, and FMB scores were higher than those at T0 (first day of admission). FSS scores in the CPR group were lower, while FMA and FMB scores were higher than those in the CR group at T1 and T2. The incidence of pneumonia was 10.00% in the CPR group and 25.00% in the CR group. The rehabilitation effective rate was 92.50% in the CPR group and 80.00% in the CR group, but the proportion of rehabilitation effect in the CPR group was higher than that in the CR group.</p><p><strong>Conclusions: </strong>CPR program improves fatigue and motor function and reduces the occurrence of SAP in AIS patients.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"208"},"PeriodicalIF":2.8,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11948786/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143718318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comparison of conventional high-flow nasal cannula vs the duet high-flow nasal cannula in extubated patients: a randomized crossover trial.","authors":"Ravindranath Tiruvoipati, Mallikarjuna Ponnapa Reddy, Eldho Paul, Sachin Gupta, Ashwin Subramaniam, David Zhang, Kavi Haji","doi":"10.1186/s40001-025-02447-0","DOIUrl":"10.1186/s40001-025-02447-0","url":null,"abstract":"<p><strong>Objectives: </strong>High-flow nasal cannulae (HFNC) are often used for oxygen delivery in intensive care units (ICU). A new configuration HNFC, the Duet HFNC, can deliver higher pressures and potentially improve gas exchange. The clinical performance, safety and patient comfort of these Duet cannula are unclear. Furthermore, there is no data on performance of conventional or Duet HFNC in patients with nasogastric tubes (NG) or between male and female patients. We compared the gas exchange, tolerance, and comfort with conventional and Duet HFNC.</p><p><strong>Research methodology: </strong>This single-center, open labeled randomized crossover study included invasively ventilated patients who were extubated. Patients were randomized to protocol A (n = 25; Duet cannula for 30 min followed by conventional HFNC for 30 min) or protocol B (n = 25; conventional followed by Duet HFNC). Subgroup analysis included comparisons between patients with and without a NG, and between male and female patients when receiving Duet and conventional HFNC. During the study period the HFNCs flow rates were set at 40 L/min with the same FiO₂ standardizing the comparisons for both cannulas.</p><p><strong>Results: </strong>There was no significant difference in PaO₂ (86.8 [22.2] vs. 88.9 [23.5]; p = 0.38), PaCO₂ (37.7 [3.9] vs. 37.8 [3.9]; p = 0.81) and SaO₂ (96.2% [2.0] vs. 96.4% [1.8]; p = 0.32), comfort and tolerance between the Duet and conventional HFNC. The subgroup analysis did not show a significant difference between these cannulas in patients with or without nasogastric tubes or between males and females.</p><p><strong>Conclusion: </strong>Duet HFNC is comparable to conventional HFNC. Further studies on higher flow rates and for longer period are warranted to determine the utility of Duet cannula.</p><p><strong>Clinical trial registration: </strong>This trial was registered with ClinicalTrials.gov (Number: NCT06398951) on 3rd May 2024- Retrospectively registered.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"205"},"PeriodicalIF":2.8,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11938778/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143718238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pregnancy is influenced by more than just embryo ploidy: a retrospective study on preimplantation genetic testing.","authors":"Sui-Bing Miao, Geng Tian, Zhen-Chuan Zhao, Xiao-Wei Wang, Jian Zhao, Cai-Ping Geng","doi":"10.1186/s40001-025-02457-y","DOIUrl":"10.1186/s40001-025-02457-y","url":null,"abstract":"<p><strong>Background: </strong>Assisted reproductive technology (ART) has been widely used to treat infertility for more than four decades, but its efficacy is still lower than expected. Therefore, further exploration of the factors that affect the pregnancy outcome of ART treatment is necessary.</p><p><strong>Materials and methods: </strong>A retrospective study of chromosome rearrangement carrier couples who requested preimplantation genetic testing (PGT) for structural rearrangements at the Fourth Hospital of Shijiazhuang was conducted between February 2019 and December 2022. Multivariate logistic regression analysis was performed to determine the risk factors for pregnancy.</p><p><strong>Results: </strong>In total, 113 couples were transferred with a single euploid blastocyst, and 77 couples achieved pregnancy. Women with good-quality embryos transferred had a higher probability of pregnancy than women with poor-quality embryos transferred (OR 6.149, 95% CI 2.026-18.658). The chance of pregnancy was higher in women with a pregnancy history than in women without a pregnancy history (OR 3.181, 95% CI 1.157-8.747). The progesterone level on the day of trigger was positively associated with pregnancy (OR 2.605, 95% CI 1.226-5.538).</p><p><strong>Conclusion: </strong>Embryo quality is significantly associated with the pregnancy rate in patients treated with PGT. Embryo ploidy is just one of the factors affecting embryo development. Future studies should focus on the molecular mechanisms of embryo development and develop corresponding detection methods.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"207"},"PeriodicalIF":2.8,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11938619/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143718314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Risk factors and prediction model for carbapenem-resistant organism infection in sepsis patients.","authors":"Ronghua Liu, Xiang Li, Jie Yang, Yue Peng, Xiaolu Liu, Chanchan Tian","doi":"10.1186/s40001-025-02448-z","DOIUrl":"10.1186/s40001-025-02448-z","url":null,"abstract":"<p><strong>Background: </strong>It aimed to identify the key risk factors associated with carbapenem-resistant organism (CRO) infections in septic patients, and subsequently develop a nomogram and assess its predictive accuracy.</p><p><strong>Methods: </strong>The study population comprised adult critically ill patients with sepsis, drawn from the MIMIC-IV 2.0 data set. The data were split into a training set and a validation set at a 7:3 ratio. Independent predictors were identified using both univariate and multivariate logistic regression models, followed by the construction of a nomogram. The predictive performance of the model was evaluated using the C-index, receiver operating characteristic (ROC) curve, area under the curve (AUC), calibration curve, and decision curve.</p><p><strong>Results: </strong>We enrolled 8814 patients, with 529 (6%) CRO-infected and 8285 (94%) non-CRO-infected. Using risk factors such as age, gender, laboratory values (WBC_max, Creatinine_max, BUN_max, Hemoglobin_min, Sodium_max), and medical conditions (COPD, hypoimmunity, diabetes), along with medications (meropenem, ceftriaxone), we developed a predictive model for CRO infection in septic patients. The model demonstrated good performance, with AUC values of 0.747 for the training set and 0.725 for the validation set. The calibration curve indicates that predicted outcomes align well with observed outcomes. The clinical decision curve results indicate that the nomogram prediction model has a high net benefit, which is clinically beneficial.</p><p><strong>Conclusions: </strong>The nomogram we have developed for predicting the risk of CRO infection in sepsis patients is reasonably accurate and reliable.</p><p><strong>Clinical trial number: </strong>Not applicable.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"201"},"PeriodicalIF":2.8,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11934461/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143699547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Uncovering the subtle relationship between vitamin D and kidney stones: a cross-sectional NHANES-based study.","authors":"Fan Zhang, Wenjian Li","doi":"10.1186/s40001-025-02474-x","DOIUrl":"10.1186/s40001-025-02474-x","url":null,"abstract":"<p><strong>Objective: </strong>This study investigated the potential correlation between serum vitamin D levels and the risk of kidney stones. To this end, the National Health and Nutrition Examination Survey (NHANES) database resources from 2007 to 2018 were utilized. The influence of other demographic characteristics, lifestyle habits, and chronic diseases on this relationship was also assessed.</p><p><strong>Methods: </strong>This study included 59,842 participants from the NHANES survey, and after exclusions, 24,323 individuals with complete data were analyzed. Logistic regression modeling assessed odds ratios (OR) and 95% confidence intervals (CI) between vitamin D levels and kidney stone risk, and multivariable adjustment models were constructed to control for potential confounders. To investigate the dose-response relationship between vitamin D and kidney stones, restricted cubic spline (RCS) modeling was employed. Subgroup and interaction analyses were also conducted.</p><p><strong>Results: </strong>The preliminary analyses indicated a statistically significant positive correlation between vitamin D levels and kidney stone risk before adjustment for potential confounding variables (OR = 1.01, P < 0.001). However, after gradual adjustment for age, gender, race, and multiple lifestyle and chronic diseases, this association became non-significant (OR = 1.00, P = 0.186). Furthermore, RCS analyses demonstrated that the non-linear relationship between vitamin D levels and kidney stone risk was no longer statistically significant after adjustment for confounders. In the subgroup analyses, only slight statistical associations were observed in the subgroups of vigorous exercise and those with diabetes, with no significant differences in the remaining subgroups.</p><p><strong>Conclusion: </strong>The present study indicates that serum vitamin D level is not an independent predictor of kidney stone risk. Rather, its effect may be co-regulated by multiple confounding factors. Further research is required to elucidate the precise mechanisms through which vitamin D contributes to kidney stone formation and to consider the combined effects of genetic polymorphisms, dose effects, and other factors.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"202"},"PeriodicalIF":2.8,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11934492/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143699637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The dual role of diabetes on oral potentially malignant disorders.","authors":"Xin Chen, Zheng Cheng, Junyu Xu, Qianyi Wang, Zhibai Zhao, Qing Cheng, Qianglin Jiang","doi":"10.1186/s40001-025-02462-1","DOIUrl":"10.1186/s40001-025-02462-1","url":null,"abstract":"<p><strong>Background: </strong>Observational studies suggest a link between diabetes and oral potentially malignant disorders (OPMDs), such as oral lichen planus (OLP) and oral leukoplakia (OLK). The causal relationship, as well as the type of diabetes that promotes OPMDs development, remains unclear. This Mendelian randomization (MR) study estimated the causal effects of diabetes-related traits on OPMDs.</p><p><strong>Methods: </strong>Large-scale genome-wide association study data on type 1 diabetes (T1D), type 2 diabetes (T2D), fasting glucose (FG), fasting insulin (FI), glycated hemoglobin (HbA1c), OLP, OLK, and actinic cheilitis (AC) were used. Causal effects were assessed using inverse-variance weighted (IVW), weighted median, and MR-Egger methods. Multivariable MR analyses evaluated the independent roles of these traits, with extensive sensitivity analyses.</p><p><strong>Results: </strong>Genetic susceptibility to T1D (IVW OR = 1.09, 95% CI 1.02-1.17, P = 0.007) and T2D (IVW OR = 0.91, 95% CI 0.86-0.97, P = 0.002) showed protective effects against AC. T1D was associated with an increased risk of OLP (IVW OR = 1.09, 95% CI 1.02-1.17, P = 0.007). The effect of T1D on AC and OLP remained robust after adjusting for FI, FG, and HbA1c, while T2D's effect on AC was not significant when considering these glycemic traits. No potential pleiotropy was detected (P > 0.05).</p><p><strong>Conclusions: </strong>T1D may have a causal role in the development of OLP independent of glycemic traits, emphasizing the need for routine oral examinations in T1D patients. Conversely, genetically predicted T1D and T2D are significantly associated with a reduced risk of AC, challenging previous assumptions and offering new insights into the relationship between diabetes and OPMDs. Further extensive investigations are required to address the limitations of this study and to clarify these associations.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"199"},"PeriodicalIF":2.8,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11931820/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143691511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reproductive health needs of incarcerated women in developed countries: a mixed-method systematic review.","authors":"Mohaddeseh Gharagozloo, Maryam Moridi, Masoud Alimardi, Zahra Behboodi Moghadam","doi":"10.1186/s40001-025-02458-x","DOIUrl":"10.1186/s40001-025-02458-x","url":null,"abstract":"<p><strong>Background: </strong>With the increasing number of women prisoners under 45 years of age, identifying the reproductive health needs of these marginalized women has become crucial for providing healthcare and improving their well-being. This systematic review aims to determine the reproductive health needs of incarcerated women in developed countries.</p><p><strong>Methods: </strong>In this systematic review, four electronic databases (PubMed, Scopus, Web of Science, and Google Scholar) were searched until January 2023. The methodological quality of the included studies was assessed using the Mixed Methods Appraisal Tool. Qualitative studies were analyzed using thematic synthesis.</p><p><strong>Results: </strong>Seventeen studies were selected and analyzed narratively. The reproductive health needs of incarcerated women were classified into four main categories: preventive care, medical care, childbearing care, and health-promoting services. Key needs included appropriate screening tests, infection control practices, medical and psychological services, pregnancy-related care, parenting services, family planning, hygiene, nutrition, and health education.</p><p><strong>Conclusion: </strong>This study highlights the need for tailored reproductive healthcare for incarcerated women. An all-inclusive strategy involving various stakeholders is required to improve the health status of these vulnerable women.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"200"},"PeriodicalIF":2.8,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11931785/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143691509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of novel inflammatory response-related biomarkers in patients with ischemic stroke based on WGCNA and machine learning.","authors":"Chenyi Huang, Dengxuan Wu, Guifen Yang, Chuchu Huang, Li Li","doi":"10.1186/s40001-025-02454-1","DOIUrl":"10.1186/s40001-025-02454-1","url":null,"abstract":"<p><strong>Background: </strong>Ischemic stroke (IS) is one of the most common causes of disability in adults worldwide. This study aimed to identify key genes related to the inflammatory response to provide insights into the mechanisms and management of IS.</p><p><strong>Methods: </strong>Transcriptomic data for IS were downloaded from the Gene Expression Omnibus (GEO) database. Weighted gene co-expression network analysis (WGCNA) and differential expression analysis were used to identify inflammation-related genes (IRGs) associated with IS. Hub IRGs were screened using Lasso, SVM-RFE, and random forest algorithms, and a nomogram diagnostic model was constructed. The diagnostic performance of the model was assessed using receiver operating characteristic (ROC) curves and calibration plots. Additionally, immune cell infiltration and potential small molecule drugs targeting IRGs were analyzed. The expression of IRG was verified by qRT-PCR in healthy controls and IS patients.</p><p><strong>Results: </strong>Nine differentially expressed IRGs were identified in IS, including NMUR1, AHR, CD68, OSM, CDKN1A, RGS1, BTG2, ATP2C1, and TLR3. Machine learning algorithms selected three hub IRGs (AHR, OSM, and NMUR1). A diagnostic model based on these three genes showed excellent diagnostic performance for IS, with an area under the curve (AUC) greater than 0.9 in both the training and validation sets. Immune infiltration analysis revealed higher levels of neutrophils and activated CD4 + T cells, and lower levels of CD8 + T cells, activated NK cells, and naive B cells in IS patients. The hub IRGs exhibited significant correlations with immune cell infiltration. Furthermore, small molecule drugs targeting hub IRGs were identified, including chrysin, piperine, genistein, and resveratrol, which have potential therapeutic effects for IS. qRT-PCR evaluation demonstrated that the levels of blood biomarkers (AHR, OSM, and NMUR1) in IS patients could serve as distinguishing indicators between IS patients and healthy controls (P < 0.05).</p><p><strong>Conclusion: </strong>This study confirmed the significant impact of IRGs on the progression of IS and provided new diagnostic and therapeutic targets for personalized treatment of IS.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"195"},"PeriodicalIF":2.8,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11929209/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143676570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical efficacy of metagenomic next-generation sequencing for the detection of pathogens in peritoneal dialysis-related peritonitis: a prospective cohort study.","authors":"Qingyan Zhang, Yuan Feng, Yangyang Xia, Qiuyuan Shao, Min Zhao, Pengfei Xu, Tianfeng Tang, Jing Liu, Bo Jin, Sixiu Liu, Chunming Jiang","doi":"10.1186/s40001-025-02322-y","DOIUrl":"10.1186/s40001-025-02322-y","url":null,"abstract":"<p><strong>Background: </strong>Metagenomic next-generation sequencing (mNGS) has been reported to improve pathogen identification in infectious diseases. This prospective cohort study aimed to explore the etiological diagnostic value of mNGS in peritoneal dialysis (PD)-related peritonitis.</p><p><strong>Methods: </strong>Patients with PD-related peritonitis were consecutively recruited at the Nephrology Department of Nanjing Drum Tower Hospital. PD effluent samples for mNGS and culture were collected simultaneously. The positive rate, detection time, and consistency of mNGS and culture were compared.</p><p><strong>Results: </strong>From August 1, 2021 to August 31, 2022, 38 patients with 41 episodes of PD-related peritonitis were enrolled. The positive rate of mNGS was higher than that of culture, although not statistically significant (92.7% vs 78.0%, P = 0.109). The average reporting time of mNGS was significantly shorter than that of culture (30.4 ± 10.5 vs 86.9 ± 22.2 h, P < 0.001). mNGS identified more co-pathogens and unusual pathogens than culture, with multiple pathogens being detected in nearly half of the samples. Among the 30 samples that tested positive by both methods, 27 (90%) showed completely (13 cases) or partly (14 cases) matched results between mNGS and culture. Fourteen patients (with 14 episodes of peritonitis) had used antibiotics within 2 weeks before specimen collection. Antibiotic usage led to a significant decrease in the culture-positive rate (57.1% vs 88.9%, P = 0.042), while the mNGS-positive rate remained unaffected (92.9% vs 92.6%, P = 1.000).</p><p><strong>Conclusions: </strong>This study revealed that mNGS exhibited higher sensitivity and shorter reporting time compared to culture in detecting pathogens in PD-related peritonitis. For samples that yielded positive results by both methods, the consistency between mNGS and culture was substantial. mNGS may offer a novel approach for the etiological diagnosis of PD-associated peritonitis, particularly in cases involving prior antibiotic use and unusual pathogens.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"198"},"PeriodicalIF":2.8,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11929341/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143691505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}