European Journal of Medical Research最新文献

{"title":"Association between asthma and thyroid function as well as thyroid hormone sensitivity indicators: an NHANES study.","authors":"Ke Li, Qiangbin Ding, Ruidong Liu, Suqiong Lin, Rongliang Qiu, Guoyang Wu","doi":"10.1186/s40001-025-03122-0","DOIUrl":"10.1186/s40001-025-03122-0","url":null,"abstract":"<p><strong>Objective: </strong>Thyroid hormones significantly influence multiple physiological systems, particularly the respiratory system. Despite limited research on asthma-thyroid associations, emerging studies have begun exploring this link. This cross-sectional study investigates relationships between asthma, thyroid function, and thyroid hormone sensitivity in U.S. adults using NHANES data.</p><p><strong>Methods: </strong>A total of 8160 participants were included in this study. Weighted analyses of data from the 2007-2012 National Health and Nutrition Examination Survey (NHANES) were performed to examine the associations between asthma, thyroid function, and thyroid hormone sensitivity indices. Subgroup analyses and ROC curve investigations were also conducted. Additionally, a retrospective cohort of 30 asthma patients and 30 non-asthmatic controls seen at Zhongshan Hospital, Xiamen University between July 2023 and July 2025 was extracted; FT3 levels were compared and asthma control was evaluated across FT3 tertiles. Thyroid function parameters assessed were as follows: free triiodothyronine (FT3), free thyroxine (FT4), total triiodothyronine (TT3), total thyroxine (TT4), thyroid-stimulating hormone (TSH), thyroid peroxidase antibody (TPOAb), thyroglobulin antibody (TgAb), and thyroglobulin (Tg). Thyroid hormone sensitivity indices-namely, the FT3/FT4 ratio, thyrotroph T4 resistance index (TT4RI), thyroid-stimulating hormone index (TSHI), and thyroid feedback quantile index (TFQI)-were calculated from serum FT3, FT4, and TSH values.</p><p><strong>Results: </strong>Asthmatics exhibited higher FT3 and FT3/FT4 levels but lower TPOAb than non-asthmatics. However, adjusted models (2 and 3) revealed an inverse association between asthma risk and FT3/FT4 (β: - 0.05, 95% CI - 0.09 to - 0.01). Quartile stratification maintained this inverse trend, with significant dose-response relationships in unadjusted Model 1 (P < 0.05). Subgroup analyses showed Mexican American asthmatics had lower FT3 and FT3/FT4 levels. ROC curves indicated superior predictive accuracy for TPOAb (AUC = 0.60) compared to FT3/FT4. Our institutional validation revealed that asthma patients had significantly lower FT3 levels than controls, and higher FT3 was associated with a lower proportion of acute exacerbations; however, the trend did not reach statistical significance.</p><p><strong>Conclusion: </strong>FT3 and FT3/FT4 levels may inversely correlate with asthma risk, though causality remains unclear due to study design limitations. Further research is warranted.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"885"},"PeriodicalIF":3.4,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12476040/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145181946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of anion gap on in-hospital mortality in trauma-induced coagulopathy patients: a MIMIC-IV study.","authors":"Xin Zuo, Huan Zuo, Pengyu Zhu","doi":"10.1186/s40001-025-03089-y","DOIUrl":"10.1186/s40001-025-03089-y","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to investigate the association between anion gap (AG) and clinical outcomes in patients with trauma-induced coagulopathy (TIC), and to evaluate the predictive value of dynamic AG trajectories for 28-day mortality.</p><p><strong>Methods: </strong>A retrospective cohort study was conducted using the MIMIC-IV database, including 1,358 TIC patients (INR > 1.4 or PTT > 40 s). Demographics, laboratory data (including AG), vital signs, and severity scores within 24 h of ICU admission were collected. Latent class growth modeling was used to identify AG trajectories at 8, 16, and 24 h. The primary outcome was 28-day mortality, analyzed via Cox regression and Kaplan-Meier survival analysis.</p><p><strong>Results: </strong>Among 1,358 patients, the 28-day mortality rate was 19.9%. Non-survivors were older and had worse vital signs, higher AG, and lower platelet and bicarbonate levels (P < 0.05). Elevated AG was independently associated with increased mortality (HR = 2.615, P < 0.001), even after multivariate adjustment. Patients with AG > 14.5 mEq/L had significantly reduced 28-day survival (P < 0.001). Four distinct AG trajectories were identified; Class 4, with persistently rising AG, showed the highest mortality risk (HR = 2.46, P = 0.033).</p><p><strong>Conclusion: </strong>AG is an independent predictor of 28-day mortality in TIC patients. AG > 14.5 mEq/L and persistently elevated trajectories are associated with poor short-term outcomes, especially in those with renal dysfunction or myocardial infarction. Dynamic AG monitoring may help identify high-risk patients early and guide clinical management.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"884"},"PeriodicalIF":3.4,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12476045/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145181881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between trace metal element levels and ideal cardiovascular health in adolescents.","authors":"Long Yang, Jiangrong Shi, Xia Shen, Jing Tang, Abudushalamu Kasimujiang, Tuohutasheng Dejite, Zhangming Deng, Tengchao Yang, Yanyang Zheng, Chenmeng Liu, Songfeng Ma, Xuefeng Shan","doi":"10.1186/s40001-025-03061-w","DOIUrl":"10.1186/s40001-025-03061-w","url":null,"abstract":"<p><strong>Backgrounds: </strong>Adolescence is a critical stage in the development of cardiovascular disease. The American Heart Association has introduced a state-of-the-art algorithm called \"Life Essential 8\" (LE8) designed to quantify cardiovascular health (CVH). Research on the association of trace metal elements with cardiovascular disease in adolescents is inconsistent. This study aims to examine the association between trace metal elements and ideal CVH in adolescents.</p><p><strong>Methods: </strong>This cross-sectional study included 5724 adolescents who participated in the National Health and Nutrition Examination Survey (NHANES) from 2005 to 2018. CVH was assessed using Life's Essential 8 (LE8) developed by the American Heart Association (AHA). Exposure factors were trace metal elements (iron, iodine, cadmium, lead, mercury, selenium, and manganese).</p><p><strong>Results: </strong>More than half of the adolescents had non-ideal CVH and only 40.8% achieved ideal CVH scores. Of the CVH measures, high blood glucose (96.1%), body mass index (81.3%), blood pressure (77.4%), and physical activity (65.0%) had high proportions of \"ideal\" values. However, diet, lipids and nicotine intake had lower rates of achieving the ideal CVH criteria (26.9%, 42.6% and 47.6%, respectively). The results of regression logistic showed that iron and mercury were negatively associated with the occurrence of non-ideal CVH (adjusted ORs of 0.997 [95% CI 0.995,0.999] and 0.913 [95% CI 0.840,0.991], and that urinary iodine, blood cadmium, lead, and selenium were positively associated with the occurrence of non-ideal CVH (adjusted ORs of 1.432 [95% CI 1.054,1.945], 3.845 [95% CI 2.278,6.491], and 1.010 [95% CI 1.003,1.017], respectively). No correlation between manganese and CVH was observed.</p><p><strong>Conclusions: </strong>There is an urgent need to intervene in the CVH of children and adolescents. It is important to emphasize the need to maintain adequate iron intake while reducing iodine, cadmium, lead, and selenium intake.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"857"},"PeriodicalIF":3.4,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12465298/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145174185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Machine learning-driven predictions of metabolic syndrome in adults: evidence from a Kurdish cohort in Iran.","authors":"Narmin Mirzaei, Shayan Mostafaei, Neda Izadi, Farid Najafi, Mitra Darbandi, Yahya Pasdar","doi":"10.1186/s40001-025-03139-5","DOIUrl":"10.1186/s40001-025-03139-5","url":null,"abstract":"<p><strong>Background: </strong>The prevalence of metabolic syndrome (MetS) is increasing worldwide. Early detection of MetS by valid and available indicators can help to prevent, control and reduce its complications. This study aimed to identify the most important anthropometric, biochemical and nutritional indices for the prediction of MetS using a machine-learning algorithm.</p><p><strong>Methods: </strong>This study was conducted with 9602 participants from the baseline data of the Ravansar Non-Communicable Disease Cohort (RaNCD), which is part of the PERSIAN study including adults aged 35-65 years. The reference model for MetS was considered according to the International Diabetes Federation (IDF) criteria. The Boruta algorithm and ROC curve analysis were used to select and assess the most important predictors of MetS.</p><p><strong>Results: </strong>The importance value (IV) for the components of the models predicting MetS was confirmed before the models were implemented. The identified model with components of age, waist circumference (WC), body mass index (BMI), fasting blood sugar (FBS), systolic-diastolic blood pressure (SBP-DBP), triglyceride, hip circumference and an AUC of 0.89 (95% CI 0.88-0.90) for men and 0.86 (95% CI 0.85-0.88) for women was the strongest model for predicting MetS risk. The AUC for the non-invasive model was 0.75 (95% CI 0.74-0.76) in the total population and has good predictive power for MetS risk with the components age, WC, BMI, SBP, DBP.</p><p><strong>Conclusions: </strong>This study showed that in addition to aggressive models, non-invasive models (anthropometric indices, blood pressure and energy intake) can be a good and convenient screening tool for predicting MetS. The models can be used in clinical diagnosis as well as in research on large populations.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"876"},"PeriodicalIF":3.4,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12465373/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145174286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic and prognostic significance of Super-enhancer-associated genes in Crohn's disease and immune regulatory roles.","authors":"Xiaojiao Wu, Fang Yan, Yang Yang, Yuanyuan Li","doi":"10.1186/s40001-025-03168-0","DOIUrl":"10.1186/s40001-025-03168-0","url":null,"abstract":"<p><strong>Background: </strong>Super-enhancers (SEs) are implicated in the regulation of the onset and progression of Crohn's disease (CD). However, the precise mechanisms remain unclear. This study aims to investigate the diagnostic potential and molecular mechanisms of SE-related genes (SERGs) as biomarkers for CD from a transcriptomics perspective.</p><p><strong>Methods: </strong>Data on CD and SERGs were retrieved from public databases. Differential expression analysis, the Maximal Clique Centrality algorithm, and two machine learning methods were applied, complemented by receiver operating characteristic analysis and expression level assessment to identify biomarkers and construct a nomogram for evaluating their diagnostic value. Enrichment and immune infiltration analyses were performed subsequently. Unsupervised clustering categorized CD samples into distinct subtypes based on the biomarkers, and functional and immune microenvironmental differences among these subtypes were examined. Immunohistochemical experiments confirmed the elevated protein expression of hub genes in colonic tissues from patients with CD relative to healthy controls (P < 0.05).</p><p><strong>Results: </strong>PAQR5, IFITM3, PSMB8, and IRF1, were identified as biomarkers for CD, demonstrating robust diagnostic performance, with area under curve (AUC) values of 0.988, 0.973, 0.959, and 0.940, respectively. When integrated into a nomogram, the AUC reached 0.991. Except for PAQR5, the other three genes showed significantly higher expression in CD samples, a trend confirmed clinically by immunohistochemical experiments. These biomarkers were involved in areas such as immune regulation, cell biology, and sensory perception. Immunoassays further elucidated the specific infiltration patterns of different immune cells in CD and highlighted the importance of these biomarkers in regulating immune cell functions. Additionally, we classified the CD samples into two subtypes, with the biomarkers exhibiting significant expression differences between the subtypes. These two subtypes possessed distinct immune microenvironments, with the biomarkers showing moderate correlation with the differential immune cells between the subtypes.</p><p><strong>Conclusion: </strong>PAQR5, IFITM3, PSMB8, and IRF1 serve as biomarkers for CD, offering substantial diagnostic and immune regulatory value. These biomarkers differentiate CD into two subtypes, providing insights for personalized therapeutic strategies.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"882"},"PeriodicalIF":3.4,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12465794/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145174252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of liposomal bupivacaine erector spinae plane block versus wound infiltration for postoperative analgesia and quality of recovery in lumbar fusion surgery: a randomized controlled trial.","authors":"Jing-Yi Niu, Yi-Di Yang, Rui-Ning Ouyang, Dong Liu, Quan Zhang, Jia-Long Qi, Guo-Song Han, Ke Zheng, Li Ma, Jun-Ma Yu, Deng-Pan Yao","doi":"10.1186/s40001-025-03174-2","DOIUrl":"10.1186/s40001-025-03174-2","url":null,"abstract":"<p><strong>Background: </strong>This study aimed to compare the efficacy of liposomal bupivacaine ultrasound-guided erector spinae plane block (ESPB) with wound infiltration (WI) on postoperative analgesia and quality of recovery in patients undergoing lumbar spinal fusion surgery.</p><p><strong>Methods: </strong>Eighty patients scheduled for lumbar fusion surgery were randomized to receive either WI (n = 40) or ultrasound-guided bilateral ESPB (n = 40). Pain intensity in the postanesthesia care unit (PACU) and at 6, 12, 24, 48 and 72 h after surgery was the primary outcome. The secondary outcomes included quality of recovery, intraoperative opioid consumption, use of rescue analgesics within 72 h and postoperative lumbar function. Adverse events were recorded.</p><p><strong>Results: </strong>Compared with the WI group, the ESPB group demonstrated lower pain scores in the PACU (median [IQR]: 2 [1,2] vs. 2 [2,3]; median difference, -1; 95% confidence interval [CI] -2 to -1; P < 0.001) and at 6 h after surgery (median [IQR]: 2 [1,2] vs. 3 [2,3]; median difference, -1; 95% CI -1 to -1; P < 0.001). There were no significant differences in VAS scores between the two groups beyond 6 h. In addition, the ESPB group had higher Quality of Recovery-15 scale scores at 72 h after surgery (mean ± SD: [119.49 ± 5.01] vs. [116.61 ± 4.33]; P = 0.009). The doses of sufentanil (median [IQR]: 20 [20,22] vs. 25 [23,30]; P < 0.001) and remifentanil (median [IQR]: 680 [560,850] vs 775 [647.5,1005]; P = 0.042) were significantly lower in the ESPB group than those in the WI group. However, there were no differences in the Oswestry Disability Index between the groups at 1 or 3 months after surgery.</p><p><strong>Conclusions: </strong>For patients undergoing lumbar spinal fusion surgery, compared with WI, ultrasound‑guided ESPB with liposomal bupivacaine provided a slight advantage in improving postoperative analgesia and quality of recovery, and reduced intraoperative opioid consumption. However, these findings probably lack clinical significance.</p><p><strong>Trial registration: </strong>This study was registered at the Chinese Clinical Trial Registry ( http://www.chictr.org.cn , ChiCTR240084425; Registration Date: May 16, 2024).</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"883"},"PeriodicalIF":3.4,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12465786/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145174260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Up-regulating methylation of CAP1 promoter can promote apoptosis and inhibit migration and invasion of lung adenocarcinoma cells.","authors":"Guoshu Li, Yunlu Gu, Kai Wang, Yongen Miao, Min Tan, Jushan Zhang, Junyong Zou, Haoxiang Li, Changhui Wang, Shuanshuan Xie","doi":"10.1186/s40001-025-03135-9","DOIUrl":"10.1186/s40001-025-03135-9","url":null,"abstract":"<p><strong>Background: </strong>Lung adenocarcinoma (LUAD) is the most common histological subtype of lung cancer, which poses a significant threat to human health. Adenylate cyclase-associated protein 1 (CAP1) is an important protein closely linked to cancer initiation and progression.</p><p><strong>Method: </strong>Target Gene fragments were amplified by PCR, and the products of 2 fragments were ligated to construct pdCas9-Dnmt3a-BSD plasmid. Stable cell lines with methylation of CAP1 promoter up-regulated were then established through transfection and screening. Cell proliferation was assessed using colony formation and proliferation assays, while apoptosis was assessed by flow cytometry. Wound healing, transwell migration, and invasion assays were conducted to evaluate cell migration and invasion. Western blot and PCR assays were used to study the expression of molecules involved in apoptosis, migration, and invasion.</p><p><strong>Result: </strong>CAP1 protein expression was higher in early-stage LUAD tissues than in adjacent normal tissues, and elevated in A549, H1299, and PC9 cells as compared to Beas-2B control cells. In addition, CAP1 promoter was abnormally hypo-methylated in LUAD cells and tissues. Up-regulating CAP1 promoter methylation through the CRISPR-dCas9-Dnmt3a system which reduced CAP1 expression can induce apoptosis via the Bax/Bcl-2/Caspase-3 pathway, and inhibited migration and invasion. We also found that the methylation of the CAP1 promoter was regulated by Dnmt3a, Tet1, and/or Tet2.</p><p><strong>Conclusion: </strong>Up-regulating CAP1 promoter methylation promotes apoptosis and inhibits migration and invasion of LUAD cells. This suggests that methylating the CAP1 promoter could be a potential therapeutic approach for early-stage LUAD.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"869"},"PeriodicalIF":3.4,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12465864/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145174287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Global Burden of Disease of interstitial lung disease and pulmonary sarcoidosis in adolescents and young adults (1990-2019), and projections for the next 30 years.","authors":"Xiaoshuang He, Lu Wang, Yu Zhao, Yuanyuan Qu, Wenyan Xin, Lina Xu, Wanyu Li, Chao Wu","doi":"10.1186/s40001-025-03141-x","DOIUrl":"10.1186/s40001-025-03141-x","url":null,"abstract":"<p><strong>Background: </strong>Interstitial lung disease (ILD) and pulmonary sarcoidosis are common respiratory diseases that are difficult to diagnose and costly to treat. Adolescents and young adults (AYAs) aged 15-39 years are frequently overlooked in global burden estimates despite their unique epidemiological and societal implications. Comprehensive global burden estimates are lacking. To address this gap, we analysed the results of the Global Burden of Disease Study (GBD) 2019, focusing on Disability-Adjusted Life Years (DALYs).</p><p><strong>Methods: </strong>Using the GBD 2019 dataset, we calculated age-standardised rate of incidence, mortality, and DALYs and their average annual percentage change from 1990 to 2019 across various factors, including sex, socio-demographic index (SDI), and geographic region. Decomposition analyses explored the impacts of population growth, changing age structures, and epidemiological changes on ILD incidence, DALYs, and mortality in AYAs globally and across different SDI and GBD regions.interstitial lung disease and pulmonary sarcoidosis among adolescents and young adults trends from 2020 to 2049 were predicted using Bayesian Age-Period-Cohort (BAPC).</p><p><strong>Results: </strong>In 2019, there were 2.6 million cases of ILD and pulmonary sarcoidosis in AYAs, and 3,428 deaths were attributed to these diseases. Age-standardised incidence and DALY rates significantly increased between 1990 and 2019. Females experienced higher rates than males. Standardised DALY rates decreased in high and middle-high SDI countries but increased in low, middle-low, and low-SDI countries. The primary reason was population growth, significantly impacting areas in the lower SDI quintiles.The BAPC model projected a gradual increase in the interstitial lung disease and pulmonary sarcoidosis in adolescents and young adults burden from 2020 to 2049.</p><p><strong>Conclusions: </strong>This study provides recent epidemiological data on the burden of ILD and pulmonary sarcoidosis in AYAs. These findings highlight the importance of targeted control measures to reduce the burden in this age group.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"878"},"PeriodicalIF":3.4,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12465843/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145174341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum miRNA-124 expression in patients diagnosed with hemorrhagic cerebrovascular accident and its clinical significance.","authors":"Kai-Li Liu, He Wang","doi":"10.1186/s40001-025-03152-8","DOIUrl":"10.1186/s40001-025-03152-8","url":null,"abstract":"<p><strong>Objectives: </strong>This study aimed to examine serum miRNA-124 expression in patients diagnosed with hemorrhagic cerebrovascular accident (CVA) and evaluate its clinical significance.</p><p><strong>Methods: </strong>A prospective observational study was conducted involving 20 patients diagnosed with hemorrhagic CVA who were admitted to the Department of Neurosurgery, Affiliated Hospital of Beihua University. Quantitative reverse transcription polymerase chain reaction was used to measure serum miRNA-124 levels within 12 h of symptom onset and on day 7 after onset.</p><p><strong>Results: </strong>Paired t test analysis yielded a test statistic of t = 3.499 (p = 0.002), indicating a statistically significant difference between the two timepoints. Serum miRNA-124 levels on day 7 were significantly higher than within 12 h of onset, possibly reflecting the progression of post-hemorrhagic cerebral edema.</p><p><strong>Conclusions: </strong>Serum miRNA-124 expression in patients diagnosed with hemorrhagic CVA was significantly higher on day 7 after onset than within 12 h. This finding aligns with previous reports suggesting a potential relationship between miRNA-124 upregulation and the development of cerebral edema after hemorrhagic CVA.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"870"},"PeriodicalIF":3.4,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12465184/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145174345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated bioinformatics analysis unravels mitochondrial-immune crosstalk and infiltration dynamics in sepsis progression.","authors":"Fanjian Meng, Anyuan Zhong, Ting Li, Yun Yang, Chen Chen, Yongkang Huang, Tong Zhou, Yongjian Pei, Minhua Shi","doi":"10.1186/s40001-025-03142-w","DOIUrl":"10.1186/s40001-025-03142-w","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Background: &lt;/strong&gt;Sepsis is a critical illness, and mitochondrial dysfunction is associated with its progression. However, the classification of mitochondrial-related differentially expressed genes (MitoDEGs) in sepsis and the immune infiltration characteristics have not been thoroughly investigated. This study aimed to explore the relevant content.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;Gene expression data were obtained from the Gene Expression Omnibus (GEO), while mitochondrial-related genes were sourced from the MitoCarta3.0 database. We applied Weighted Gene Co-expression Network Analysis (WGCNA) to identify Sepsis-related MitoDEGs (Se-MitoDEGs), and utilized unsupervised clustering analysis to categorize sepsis samples into distinct clusters. Machine learning algorithms identified hub Se-MitoDEGs, and a validation set and a nomogram for sepsis diagnosis were established. The CIBERSORT algorithm was employed to investigate immune infiltration characteristics in sepsis and their association with hub Se-MitoDEGs. The expression levels of relevant genes were evaluated in peripheral blood samples from septic patients and normal controls through quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR). Associated transcription factors, miRNAs, and drugs were constructed into a diagram via NetworkAnalyst and Comparative Toxicogenomics Database (CTD).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;15 Se-MitoDEGs exhibited differential expression between septic and normal samples. Immune infiltration analysis demonstrated significant increases in neutrophils, activated mast cells, and M0 macrophages among septic patients compared to control subjects. We categorized sepsis samples into two clusters; most hub genes in cluster 2 (C2) were highly expressed, exhibiting low immune infiltration and immune score. Some differences were observed in the pathways between the two clusters. By utilizing machine learning techniques and the validation set, MSRB2, TSPO, and BLOC1S1 were identified, and a nomogram of the three genes exhibited a substantial area under the curve (AUC) of 0.886, and the AUC for the validation set was recorded at 0.866, highlighting the robustness of our predictive model. Survival analysis found that low expression of TSPO and high expression of MSRB2 in peripheral blood were negatively correlated with the 28-day survival rate of septic patients. qRT-PCR validation indicated that the expression levels of these three hub genes are consistent with our bioinformatics analysis results. Associated small molecules, including Estradiol, pirinixic acid, and Valproic acid, are potential therapeutic drugs for sepsis.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusion: &lt;/strong&gt;By integrating bioinformatics with machine learning models, we identified three mitochondrial and immune-related biomarkers (MSRB2, TSPO, and BLOC1S1) with diagnostic value for sepsis. These biomarkers provide new insights into subtype stratification, immune infiltration characteristi","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"863"},"PeriodicalIF":3.4,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12465656/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145174331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}