Environmental microbiology最新文献_第7页

Cold Surface Waters of the Sub-Antarctic Pacific Ocean Support High Cyanophage Abundances and Infection Levels 亚南极太平洋寒冷的表层水支持高的噬藻丰度和感染水平

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-01-10 DOI: 10.1111/1462-2920.70031

Camelia Shopen Gochev, David Demory, Adriana Lopes dos Santos, Michael C. G. Carlson, Andrés Gutiérrez-Rodríguez, Joshua S. Weitz, Debbie Lindell

{"title":"Cold Surface Waters of the Sub-Antarctic Pacific Ocean Support High Cyanophage Abundances and Infection Levels","authors":"Camelia Shopen Gochev, David Demory, Adriana Lopes dos Santos, Michael C. G. Carlson, Andrés Gutiérrez-Rodríguez, Joshua S. Weitz, Debbie Lindell","doi":"10.1111/1462-2920.70031","DOIUrl":"10.1111/1462-2920.70031","url":null,"abstract":"Cyanobacterial distributions are shaped by abiotic factors including temperature, light and nutrient availability as well as biotic factors such as grazing and viral infection. In this study, we investigated the abundances of T4-like and T7-like cyanophages and the extent of picocyanobacterial infection in the cold, high-nutrient-low-chlorophyll, sub-Antarctic waters of the southwest Pacific Ocean during austral spring. Synechococcus was the dominant picocyanobacterium, ranging from 4.7 × 103 to 1.2 × 105 cells∙mL−1, while Prochlorococcus abundances were relatively low overall, ranging from 1.0 × 103 to 3.9 × 104 cells∙mL−1. Using taxon-specific, single-virus and single-cell polony methods, we found that cyanophages were on average 15-fold, and up to 50-fold, more abundant than cyanobacteria in these waters. T4-like cyanophages (ranging from 1.7 × 105 to 6.5 × 105 phage·mL−1) were 2.7-fold more abundant than T7-like cyanophages (ranging from 3.1 × 104 to 2.8 × 105 phage·mL−1). Picocyanobacteria were primarily infected by T4-like cyanophages with more Synechococcus (4.8%–12.1%) infected than Prochlorococcus (2.5%–6.2%), whereas T7-like cyanophages infected less than 1% of both genera. These infection levels translated to daily mortality in the range of 5.7%–26.2% and 2.9%–14.3% of the standing stock of Synechococcus and Prochlorococcus, respectively. Our findings suggest that T4-like cyanophages are significant agents of cyanobacterial mortality in the cold, low-iron, sub-Antarctic waters of the South Pacific Ocean.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70031","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142961910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proximity-Ligation Metagenomic Sequence Analysis Reveals That the Antibiotic Resistome Makes Significant Transitions During Municipal Wastewater Treatment 接近-结扎宏基因组序列分析揭示抗生素抗性组在城市污水处理过程中发生重大转变

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-01-10 DOI: 10.1111/1462-2920.70036

Cassandra B. McCorison, Taegyu Kim, Justin J. Donato, Timothy M. LaPara

{"title":"Proximity-Ligation Metagenomic Sequence Analysis Reveals That the Antibiotic Resistome Makes Significant Transitions During Municipal Wastewater Treatment","authors":"Cassandra B. McCorison, Taegyu Kim, Justin J. Donato, Timothy M. LaPara","doi":"10.1111/1462-2920.70036","DOIUrl":"10.1111/1462-2920.70036","url":null,"abstract":"Shotgun and proximity-ligation metagenomic sequencing were used to generate thousands of metagenome assembled genomes (MAGs) from the untreated wastewater, activated sludge bioreactors, and anaerobic digesters from two full-scale municipal wastewater treatment facilities. Analysis of the antibiotic resistance genes (ARGs) in the pool of contigs from the shotgun metagenomic sequences revealed significantly different relative abundances and types of ARGs in the untreated wastewaster compared to the activated sludge bioreactors or the anaerobic digesters (p < 0.05). In contrast, these results were statistically similar when comparing the ARGs in the pool of MAGs, suggesting that proximity-ligation metagenomic sequencing is particularly useful for pairing ARGs with their hosts but less adept at discerning quantitative differences in ARG types and relative abundances. For example, numerous MAGs of the genera Acinetobacter, Enterococcus, Klebsiella and Pseudomonas were identified in the untreated wastewater, many of which harboured plasmid-borne and/or chromosomal-borne ARGs; none of these MAGs, however, were detected in the activated sludge bioreactors or anaerobic digesters. In conclusion, this research demonstrates that the antibiotic resistome undergoes significant transitions in both the relative abundance and the host organisms during the municipal wastewater treatment process.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70036","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142961301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential Responses of Methylobacterium and Sphingomonas Species to Multispecies Interactions in the Phyllosphere 甲基细菌和鞘氨单胞菌对层状圈内多物种相互作用的差异反应

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-01-10 DOI: 10.1111/1462-2920.70025

R. O. Schlechter, M. N. P. Remus-Emsermann

{"title":"Differential Responses of Methylobacterium and Sphingomonas Species to Multispecies Interactions in the Phyllosphere","authors":"R. O. Schlechter, M. N. P. Remus-Emsermann","doi":"10.1111/1462-2920.70025","DOIUrl":"10.1111/1462-2920.70025","url":null,"abstract":"The leaf surface, known as the phylloplane, presents an oligotrophic and heterogeneous environment due to its topography and uneven distribution of resources. Although it is a challenging environment, leaves support abundant bacterial communities that are spatially structured. However, the factors influencing these spatial distribution patterns are not well understood. To study the changes in population density and spatial distribution of bacteria in synthetic communities, the behaviour of two common bacterial groups in the Arabidopsis thaliana leaf microbiota—Methylobacterium (methylobacteria) and Sphingomonas (sphingomonads)—was examined. Using synthetic communities consisting of two or three species, the hypothesis was tested that the presence of a third species affects the density and spatial interaction of the other two species. Results indicated that methylobacteria exhibit greater sensitivity to changes in population densities and spatial patterns, with higher intra-genus competition and lower densities and aggregation compared to sphingomonads. Pairwise comparisons were insufficient to explain the shifts observed in three-species communities, suggesting that higher-order interactions influence the structuring of complex communities. This emphasises the role of multispecies interactions in determining spatial patterns and community dynamics on the phylloplane.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70025","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142961302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Analysis of Protist Communities in Oilsands Tailings Using Amplicon Sequencing and Metagenomics 油砂尾矿中原生生物群落的扩增子测序与宏基因组学比较分析

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-01-10 DOI: 10.1111/1462-2920.70029

Kristína Záhonová, Harpreet Kaur, Chantel C. Furgason, Angela V. Smirnova, Peter F. Dunfield, Joel B. Dacks

{"title":"Comparative Analysis of Protist Communities in Oilsands Tailings Using Amplicon Sequencing and Metagenomics","authors":"Kristína Záhonová, Harpreet Kaur, Chantel C. Furgason, Angela V. Smirnova, Peter F. Dunfield, Joel B. Dacks","doi":"10.1111/1462-2920.70029","DOIUrl":"10.1111/1462-2920.70029","url":null,"abstract":"The Canadian province of Alberta contains substantial oilsands reservoirs, consisting of bitumen, clay and sand. Extracting oil involves separating bitumen from inorganic particles using hot water and chemical diluents, resulting in liquid tailings waste with ecotoxicologically significant compounds. Ongoing efforts aim to reclaim tailings-affected areas, with protist colonisation serving as one assessment method of reclamation progress. Oilsands-associated protist communities have mainly been evaluated using amplicon sequencing of the 18S rRNA V4 region; however, this barcode may overlook important protist groups. This study examined how community assessment methods between the V4 and V9 regions differ in representing protist diversity across four oilsands-associated environments. The V9 barcode identified more operational taxonomical units (OTUs) for Discoba, Metamonada and Amoebozoa compared with the V4. A comparative shotgun metagenomics approach revealed few eukaryotic contigs but did recover a complete Paramicrosporidia mitochondrial genome, only the second publicly available from microsporidians. Both V4 and V9 markers were informative for assessing community diversity in oilsands-associated environments and are most effective when combined for a comprehensive taxonomic estimate, particularly in anoxic environments.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70029","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142961300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mosquitoes Reared in Nearby Insectaries at the Same Institution Have Significantly Divergent Microbiomes 在同一机构附近的昆虫饲养场饲养的蚊子有明显不同的微生物组

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-01-08 DOI: 10.1111/1462-2920.70027

Laura E. Brettell, Ananya F. Hoque, Tara S. Joseph, Vishaal Dhokiya, Emily A. Hornett, Grant L. Hughes, Eva Heinz

{"title":"Mosquitoes Reared in Nearby Insectaries at the Same Institution Have Significantly Divergent Microbiomes","authors":"Laura E. Brettell, Ananya F. Hoque, Tara S. Joseph, Vishaal Dhokiya, Emily A. Hornett, Grant L. Hughes, Eva Heinz","doi":"10.1111/1462-2920.70027","DOIUrl":"10.1111/1462-2920.70027","url":null,"abstract":"The microbiome influences critical aspects of mosquito biology and variations in microbial composition can impact the outcomes of laboratory studies. To investigate how biotic and abiotic conditions in an insectary affect the composition of the mosquito microbiome, a single cohort of Aedes aegypti eggs was divided into three batches and transferred to three different climate-controlled insectaries within the Liverpool School of Tropical Medicine. The bacterial microbiome composition was compared as mosquitoes developed, the microbiome of the mosquitoes' food sources was characterised, environmental conditions over time in each insectary were measured, and mosquito development and survival were recorded. While developmental success was similar across all three insectaries, differences in microbiome composition were observed between mosquitoes from each insectary. Environmental conditions and bacterial input via food sources varied between insectaries, potentially contributing to the observed differences in microbiome composition. At both adult and larval stages, specific members of the mosquito microbiome were associated with particular insectaries; the insectary with less stable and cooler conditions resulted in a slower pupation rate and higher diversity of the larval microbiome. These findings underscore that even minor inconsistencies in rearing conditions can affect the composition of the mosquito microbiome, which may influence experimental outcomes.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70027","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142936966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New Strains of the Deep Branching Streptophyte Streptofilum: Phylogenetic Position, Cell Biological and Ecophysiological Traits, and Description of Streptofilum arcticum sp. nov 深分枝链霉菌新菌株：系统发育位置、细胞生物学和生态生理特性及北极链霉菌描述。11月

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-01-08 DOI: 10.1111/1462-2920.70033

Karin Glaser, Tatiana Mikhailyuk, Charlotte Permann, Andreas Holzinger, Ulf Karsten

{"title":"New Strains of the Deep Branching Streptophyte Streptofilum: Phylogenetic Position, Cell Biological and Ecophysiological Traits, and Description of Streptofilum arcticum sp. nov","authors":"Karin Glaser, Tatiana Mikhailyuk, Charlotte Permann, Andreas Holzinger, Ulf Karsten","doi":"10.1111/1462-2920.70033","DOIUrl":"10.1111/1462-2920.70033","url":null,"abstract":"Streptofilum capillatum was recently described and immediately caught scientific attention, because it forms a phylogenetically deep branch in the streptophytes and is characterised by a unique cell coverage composed of piliform scales. Its phylogenetic position and taxonomic rank are still controversial discussed. In the present study, we isolated further strains of Streptofilum from biocrusts in sand dunes and Arctic tundra soil. Molecular and morphological characterisation including transmission electron microscopy confirmed that both new strains belong to Streptofilum. The Arctic strain is described as a new species, Streptofilum arcticum sp. nov., based on molecular differences, a specific sarcinoid morphology and unique ultrastructure with massive cell coverage composed of pili-shaped scales. A comprehensive characterisation of the ecophysiological traits of both new Streptofilum isolates and the original one revealed a broad temperature tolerance, a rapid recovery of photosynthetic performance after desiccation, an efficient photosynthesis at low light and a tolerance to high-light conditions. In addition, Streptofilum could cope with UV irradiation, but only S. capillatum grew under UV exposure. All Streptofilum strains are well-adapted to water-deprived terrestrial habitats such as biocrusts. From this study it can be concluded that already early-branching streptophytes were able to tolerate terrestrial conditions.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70033","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142936965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Novel Lineage of Endosymbiotic Actinomycetales: Genome Reduction and Acquisition of New Functions in Bifidobacteriaceae Associated With Termite Gut Flagellates 一个新的内共生放线菌谱系：与白蚁肠道鞭毛虫相关的双歧杆菌科基因组还原和新功能的获得

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-01-08 DOI: 10.1111/1462-2920.70010

Joana Kästle Silva, Vincent Hervé, Undine S. Mies, Katja Platt, Andreas Brune

{"title":"A Novel Lineage of Endosymbiotic Actinomycetales: Genome Reduction and Acquisition of New Functions in Bifidobacteriaceae Associated With Termite Gut Flagellates","authors":"Joana Kästle Silva, Vincent Hervé, Undine S. Mies, Katja Platt, Andreas Brune","doi":"10.1111/1462-2920.70010","DOIUrl":"10.1111/1462-2920.70010","url":null,"abstract":"Cellulolytic flagellates are essential for the symbiotic digestion of lignocellulose in the gut of lower termites. Most species are associated with host-specific consortia of bacterial symbionts from various phyla. 16S rRNA-based diversity studies and taxon-specific fluorescence in situ hybridization revealed a termite-specific clade of Actinomycetales that colonise the cytoplasm of Trichonympha spp. and other gut flagellates, representing the only known case of intracellular Actinomycetota in protists. Comparative analysis of eleven metagenome-assembled genomes from lower termites allowed us to describe them as new genera of Bifidobacteriaceae. Like the previously investigated Candidatus Ancillula trichonymphae, they ferment sugars via the bifidobacterium shunt but, unlike their free-living relatives, experienced significant genome erosion. Additionally, they acquired new functions by horizontal gene transfer from other gut bacteria, including the capacity to produce hydrogen. Members of the genus Ancillula (average genome size 1.56 ± 0.2 Mbp) retained most pathways for the synthesis of amino acids, including a threonine/serine exporter, providing concrete evidence for the basis of the mutualistic relationship with their host. By contrast, Opitulatrix species (1.23 ± 0.1 Mbp) lost most of their biosynthetic capacities, indicating that an originally mutualistic symbiosis is on the decline.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70010","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142936539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Niche Partitioning and Intraspecific Variation of Thaumarchaeota in Deep Ocean Sediments 深海沉积物中太古菌的生态位分配及种内变异

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-01-08 DOI: 10.1111/1462-2920.70018

Ronghua Liu, Xinxin He, Gaoyang Ren, Da-Wei Li, Meixun Zhao, Laura Lehtovirta-Morley, Jonathan D. Todd, Xiao-Hua Zhang, Jiwen Liu

{"title":"Niche Partitioning and Intraspecific Variation of Thaumarchaeota in Deep Ocean Sediments","authors":"Ronghua Liu, Xinxin He, Gaoyang Ren, Da-Wei Li, Meixun Zhao, Laura Lehtovirta-Morley, Jonathan D. Todd, Xiao-Hua Zhang, Jiwen Liu","doi":"10.1111/1462-2920.70018","DOIUrl":"10.1111/1462-2920.70018","url":null,"abstract":"<div>\u0000 \u0000 Deep-sea sediments contain a large number of Thaumarchaeota that are phylogenetically distinct from their pelagic counterparts. However, their ecology and evolutionary adaptations are not well understood. Metagenomic analyses were conducted on samples from various depths of a 750-cm sediment core collected from the Mariana Trench Challenger Deep. The abundance of Thaumarchaeota and archaeal amoA generally decreased with depth, except for an unexpected peak midway through the core. The thaumarchaeotal metagenome-assembled genomes were classified into diverse phylogenetic clusters associated with amoA-NP-γ, amoA-NP-θ, and amoA-NP-δ of ammonia-oxidising Thaumarchaeota and non-ammonia-oxidising lineages. The most abundant group was within amoA-NP-γ, which is usually found in coastal and shallow habitats, indicating potential niche expansion from marine shallow to hadal environments. This benthic group showed within-species genomic variations compared to the previously identified Hadal water group, suggesting microdiversification of hadal Thaumarchaeota along with niche separation between benthic and pelagic environments. Evolutionary adaptations associated with the benthic-to-pelagic transition included reduced genome size, loss of motility/cell adhesion, altered energy metabolism, and different mechanisms for substrate acquisition and regulation (e.g., ammonium). These findings offer new insights into the evolution of hadal Thaumarchaeota and demonstrate, for the first time, intraspecies-level genomic variation in Thaumarchaeota related to the benthic-versus-pelagic niche partitioning in the deep ocean.\u0000 </div>","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142936540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identifying Bacteria Responsible for Non-Sulphate-Based Hydrogen Sulphide Production in Aquaculture 鉴定水产养殖中产生非硫酸盐硫化氢的细菌

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-01-08 DOI: 10.1111/1462-2920.70024

Alexandre Nguyen-tiêt, Fernando Puente-Sánchez, Stefan Bertilsson, Sanni L. Aalto

{"title":"Identifying Bacteria Responsible for Non-Sulphate-Based Hydrogen Sulphide Production in Aquaculture","authors":"Alexandre Nguyen-tiêt, Fernando Puente-Sánchez, Stefan Bertilsson, Sanni L. Aalto","doi":"10.1111/1462-2920.70024","DOIUrl":"10.1111/1462-2920.70024","url":null,"abstract":"<div>\u0000 \u0000 The unintended microbiological production of hydrogen sulphide (H2S) poses a significant challenge in engineered systems, including sewage treatment plants, landfills and aquaculture systems. Although sulphur-rich amino acids and other substrates conducive to non-sulphate-based H2S production are frequently present, the capacity and potential of various microorganisms to perform sulphate-free H2S production remain unclear. In this study, we identify the identity, activity and genomic characteristics of bacteria that degrade cysteine to produce H2S in anaerobic enrichment bioreactors seeded with material from aquaculture systems. Our comparison with canonical sulphate-reducing bacteria reveals that both sulphur sources contribute to microbial H2S production, with cysteine facilitating a more rapid process compared to sulphate. 16S rRNA amplicon sequencing and metagenomic analysis identified four bacterial families—Dethiosulfatibacteraceae, Fusobacteriaceae, Vibrionaceae and Desulfovibrionaceae—as central to non-sulphate H2S production. Metagenome- and metatranscriptome-assembled genomes elucidated the primary cysteine degradation pathway mediated by cysteine desulphidase cyuA and indicated that some bacteria may also utilise cysteine as a carbon source in sulphate-based H2S production.\u0000 </div>","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142937630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oak Wilt Disease May Reduce the Initial Decay Rate of Dead Quercus serrata Stems by Altering Fungal Communities in the Wood 栎树枯萎病可能通过改变栎树木材中的真菌群落来降低栎树死茎的初始腐烂率

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-01-08 DOI: 10.1111/1462-2920.70026

Yu Fukasawa, Satsuki Kimura, Yuji Kominami, Masahiro Takagi, Kimiyo Matsukura, Kobayashi Makoto, Satoshi N. Suzuki, Shuhei Takemoto, Nobuaki Tanaka, Mayuko Jomura, Kohmei Kadowaki, Masayuki Ushio, Haruo Kinuura, Satoshi Yamashita

{"title":"Oak Wilt Disease May Reduce the Initial Decay Rate of Dead Quercus serrata Stems by Altering Fungal Communities in the Wood","authors":"Yu Fukasawa, Satsuki Kimura, Yuji Kominami, Masahiro Takagi, Kimiyo Matsukura, Kobayashi Makoto, Satoshi N. Suzuki, Shuhei Takemoto, Nobuaki Tanaka, Mayuko Jomura, Kohmei Kadowaki, Masayuki Ushio, Haruo Kinuura, Satoshi Yamashita","doi":"10.1111/1462-2920.70026","DOIUrl":"10.1111/1462-2920.70026","url":null,"abstract":"Oak wilt causes severe dieback of Quercus serrata, a dominant tree species in the lowlands across Japan. This study evaluated the effects of oak wilt on the wood-inhabiting fungal community and the decay rate of deadwood using a field monitoring experiment. We analysed the fungal metabarcoding community from 1200 wood samples obtained from 120 experimental logs from three forest sites at five different time points during the initial 1.5 years of the experiment. Death due to wilt significantly influenced the fungal community composition and reduced fungal diversity, likely due to the dominance of a limited number of species. The operational taxonomic unit richness, occurrence frequency, and DNA copy number of white rot fungi were also enhanced on the logs killed by wilt, depending on the sites. Structural equation modelling suggested that the wilt-initiated changes in the fungal community reduced the decay rate of oak logs. Temperature and wood moisture also affected the fungal community and log decomposition. These results suggest that, in addition to the direct effect of climate, oak wilt indirectly affects log decomposition by structuring the fungal community. Continuous monitoring is essential to evaluate the longer-term effects of oak wilt on the fungal decomposition of wood.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70026","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142936542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0