Environmental microbiology最新文献_第7页

High-Throughput Tn-Seq Screens Identify Both Known and Novel Pseudomonas putida KT2440 Genes Involved in Metal Tolerance 高通量n- seq筛选鉴定已知和新的恶臭假单胞菌KT2440基因参与金属耐受性

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-04-29 DOI: 10.1111/1462-2920.70095

Kevin Royet, Laura Kergoat, Stefanie Lutz, Charlotte Oriol, Nicolas Parisot, Christian Schori, Christian H. Ahrens, Agnes Rodrigue, Erwan Gueguen

{"title":"High-Throughput Tn-Seq Screens Identify Both Known and Novel Pseudomonas putida KT2440 Genes Involved in Metal Tolerance","authors":"Kevin Royet, Laura Kergoat, Stefanie Lutz, Charlotte Oriol, Nicolas Parisot, Christian Schori, Christian H. Ahrens, Agnes Rodrigue, Erwan Gueguen","doi":"10.1111/1462-2920.70095","DOIUrl":"https://doi.org/10.1111/1462-2920.70095","url":null,"abstract":"Industrial and urban activities release toxic chemical waste into the environment. Pseudomonas putida, a soil bacterium, is known to degrade hydrocarbons and xenobiotics, and possesses numerous genes associated with heavy metal tolerance. Most studies on metal tolerance in P. putida focus solely on over- or underexpressed genes, potentially overlooking important genes with unchanged expression. This study employed a Tn-seq approach to identify the essential genes required for P. putida growth under metal stress. This method enables the identification of mutants with altered fitness in the presence of excess metals. The screen successfully identified a number of known genes implicated in metal resistance, including czcA-1, cadA-3, cadR, and pcoA2, thereby validating the approach. Further analyses using targeted mutagenesis and complementation assays revealed PP_5337 as a putative transcriptional regulator involved in copper tolerance and the two-component system RoxSR (PP_0887/PP_0888) as a key determinant of cadmium tolerance. Additionally, PP_1663 and PP_5002 were identified as contributing to cadmium and cobalt tolerance, respectively. This study provides the first evidence linking these genes to metal tolerance, highlighting gaps in our understanding of metal tolerance mechanisms in P. putida and demonstrating the utility of Tn-seq for identifying novel tolerance determinants.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 5","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70095","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143889165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Paradox of the Sub-Plankton: Plausible Mechanisms and Open Problems Underlying Strain-Level Diversity in Microbial Communities 浮游亚生物的悖论：微生物群落中菌株水平多样性的合理机制和开放问题

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-04-23 DOI: 10.1111/1462-2920.70094

Akshit Goyal, Griffin Chure

{"title":"Paradox of the Sub-Plankton: Plausible Mechanisms and Open Problems Underlying Strain-Level Diversity in Microbial Communities","authors":"Akshit Goyal, Griffin Chure","doi":"10.1111/1462-2920.70094","DOIUrl":"https://doi.org/10.1111/1462-2920.70094","url":null,"abstract":"Microbial communities are often complex and highly diverse, typically with dozens of species sharing spatially-restricted environments. Within these species, genetic and ecological variation often exists at a much finer scale, with closely related strains coexisting and competing. While the coexistence of strains in communities has been heavily explored over the past two decades, we have no self-consistent theory of how this diversity is maintained. This question challenges our conventional understanding of ecological coexistence, typically framed around species with clear phenotypic and ecological differences. In this review, we synthesise plausible mechanisms underlying strain-level diversity (termed microdiversity), focusing on niche-based mechanisms such as nutrient competition, neutral mechanisms such as migration, and evolutionary mechanisms such as horizontal gene transfer. We critically assess the strengths and caveats of these mechanisms, acknowledging key gaps that persist in linking genetic similarity to ecological divergence. Finally, we highlight how the origin and maintenance of microdiversity could pose a major challenge to conventional ecological thinking. We articulate a call-to-arms for a dialogue between well-designed experiments and new theoretical frameworks to address this grand conceptual challenge in understanding microbial biodiversity.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 4","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70094","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143865709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic Segregation and Functional Gene Clusters in Anaerobic Digestion Consortia 厌氧消化菌群的代谢分离和功能基因簇

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-04-22 DOI: 10.1111/1462-2920.70091

Yubo Wang, Ruoqun Zhang, Chunxiao Wang, Weifu Yan, Tong Zhang, Feng Ju

{"title":"Metabolic Segregation and Functional Gene Clusters in Anaerobic Digestion Consortia","authors":"Yubo Wang, Ruoqun Zhang, Chunxiao Wang, Weifu Yan, Tong Zhang, Feng Ju","doi":"10.1111/1462-2920.70091","DOIUrl":"https://doi.org/10.1111/1462-2920.70091","url":null,"abstract":"<div>\u0000 \u0000 A combined enrichment experiment and genome-centric meta-omics analysis demonstrated that metabolic specificity, rather than flexibility, governs the anaerobic digestion (AD) ecosystem. This study provides new insights into interspecies electron transfer in the AD process, highlighting a segregation in the metabolism of H2 and formate. Our findings show that H2 acts as the primary electron sink for recycling redox cofactors, including NAD+ and oxidised ferredoxin (Fdox), during primary fermentation, while formate is the dominant electron carrier in secondary fermentation, especially under conditions with elevated H2 concentrations. Importantly, no evidence of biochemical interconversion between H2 and formate was identified in the primary fermenting bacteria or in syntrophs enriched in this study. This segregation of H2 and formate metabolism likely benefits the anaerobic oxidation of butyrate and propionate with a higher tolerance to H2 accumulation. Moreover, this study highlights the functional partitioning among microbial populations in key AD niches: primary fermentation, secondary fermentation (syntrophic acetogenesis), hydrogenotrophic methanogenesis, and acetoclastic methanogenesis. Genome-centric analysis of the AD microbiome identified several key functional gene clusters, which could enhance genome-centric genotype–phenotype correlations, particularly for strict anaerobes that are difficult to isolate and characterise in pure culture.\u0000 </div>","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 4","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143861512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Warming is Associated With More Encoded Antimicrobial Resistance Genes and Transcriptions Within Five Drug Classes in Soil Bacteria: A Case Study and Synthesis 变暖与土壤细菌中5种药物类别中更多编码的抗微生物药物抗性基因和转录相关：一个案例研究和综合

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-04-22 DOI: 10.1111/1462-2920.70097

Melanie T. Hacopian, Alberto Barrón-Sandoval, Adriana L. Romero-Olivares, Renaud Berlemont, Kathleen K. Treseder

{"title":"Warming is Associated With More Encoded Antimicrobial Resistance Genes and Transcriptions Within Five Drug Classes in Soil Bacteria: A Case Study and Synthesis","authors":"Melanie T. Hacopian, Alberto Barrón-Sandoval, Adriana L. Romero-Olivares, Renaud Berlemont, Kathleen K. Treseder","doi":"10.1111/1462-2920.70097","DOIUrl":"https://doi.org/10.1111/1462-2920.70097","url":null,"abstract":"The effect of warming on anti-microbial resistance (AMR) genes in the environment has critical implications for public health but is little studied. We collected published soil bacterial genomes from the BV-BRC database and tested the correlation between reported optimal growth temperature and the number of encoded AMR genes. Furthermore, we tested the relationship between temperature and AMR gene transcription in a natural ecosystem by analysing soil transcriptomes from a warming manipulation experiment in an Alaskan boreal forest. We hypothesised that there is a positive relationship between warming and AMR prevalence in gene content in bacterial genomes and transcriptomic sequences, and that this effect would vary by drug class. Regarding the bacterial genomes, we found a positive relationship between the fraction of encoded AMR genes and the reported optimal temperature of soil bacteria. The drug classes tetracycline and lincosamide/macrolide/streptogramin had the strongest positive relationship with reported optimal temperature. For the case study in a natural ecosystem, we found 61 significantly upregulated AMR gene-associated transcripts spanning eight drug classes in warmed plots. In the Alaskan soil samples, we found that warming elicited the strongest positive effect on transcripts targeting lincosamide/streptogramin, beta-lactam and phenicol/quinolone antibiotics. Overall, higher temperatures were linked to AMR gene prevalence.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 4","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70097","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143861513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ZfpA-Dependent Quorum Sensing Shifts in Morphology and Secondary Metabolism in Aspergillus flavus zfpa依赖性群体感应在黄曲霉形态和次生代谢中的变化

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-04-22 DOI: 10.1111/1462-2920.70100

Benjamin Otoo, Dante G. Calise, Sung Chul Park, Jin Woo Bok, Nancy P. Keller, Mira Syahfriena Amir Rawa

{"title":"ZfpA-Dependent Quorum Sensing Shifts in Morphology and Secondary Metabolism in Aspergillus flavus","authors":"Benjamin Otoo, Dante G. Calise, Sung Chul Park, Jin Woo Bok, Nancy P. Keller, Mira Syahfriena Amir Rawa","doi":"10.1111/1462-2920.70100","DOIUrl":"https://doi.org/10.1111/1462-2920.70100","url":null,"abstract":"Development of the fungal pathogen Aspergillus flavus involves the balance of asexual spores (conidia) and overwintering hardened hyphal masses (sclerotia). This balance is achieved by an oxylipin-based density-dependent mechanism regulating the switch from sclerotia to conidia as population density increases in A. flavus. Here, we show the transcription factor ZfpA, required for normal oxylipin synthesis, regulates the morphology switch. ZfpA overexpression (OE::zfpA) accelerates the shift leading to increased conidial production and reduced sclerotial production under conditions normally supporting sclerotia formation. In contrast, zfpA deletion (ΔzfpA) produces more sclerotia than wild-type control. These morphology changes are coupled with changes in tissue-specific secondary metabolites. Specifically, the production of four sclerotial metabolites (oxyasparasone A, hydroxyaflatrem, aflavinine, and kotanin) decreases in OE::zfpA whereas the hyphal metabolite aspergillic acid is upregulated in this mutant. Chemical profiling of OE::zfpA compared to a double mutant where the aspergillic acid non-ribosomal synthetase was deleted in the OE::zfpA background confirmed synthesis of known aspergillic acid pathway products as well as putative Val-derived pyrazinones involved in metal chelation. These findings offer valuable insights into the quorum sensing networks connecting fungal development and tissue-specific secondary metabolite production.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 4","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70100","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143861635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proteomic Analysis of Marine Bacteriophages: Structural Conservation, Post-Translational Modifications, and Phage–Host Interactions 海洋噬菌体的蛋白质组学分析：结构保护、翻译后修饰和噬菌体-宿主相互作用

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-04-22 DOI: 10.1111/1462-2920.70099

Shuzhen Wei, Anan Wang, Lanlan Cai, Ruijie Ma, Longfei Lu, Jiangtao Li, Rui Zhang

{"title":"Proteomic Analysis of Marine Bacteriophages: Structural Conservation, Post-Translational Modifications, and Phage–Host Interactions","authors":"Shuzhen Wei, Anan Wang, Lanlan Cai, Ruijie Ma, Longfei Lu, Jiangtao Li, Rui Zhang","doi":"10.1111/1462-2920.70099","DOIUrl":"https://doi.org/10.1111/1462-2920.70099","url":null,"abstract":"Marine bacteriophages, the most abundant biological entities in marine ecosystems, are essential in biogeochemical cycling. Despite extensive genomic data, many phage genes remain uncharacterised, creating a gap between genomic diversity and gene function knowledge. This gap limits our understanding of phage life cycles, assembly, and host interactions. In this study, we used mass spectrometry to profile the proteomes of 13 marine phages from diverse lifestyles and hosts. The analysis accurately annotated hypothetical genes, mapped virion protein arrangements, and revealed structural similarities among phages infecting the same host, particularly in tail fibre proteins. Protein structure comparisons showed conservation and variability in head and tail proteins, particularly in key domains involved in virion stabilisation and host recognition. For the first time, we identified post-translational modifications (PTMs) in marine phage proteins, which may enhance phage adaptability and help evade host immune systems. These findings suggest that phages optimise their infection strategies through structural variations and PTM modifications, improving their adaptability and host interactions.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 4","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70099","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143861634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Methanol and Carbon Monoxide Metabolism of the Thermophile Moorella caeni 嗜热菌墨氏菌的甲醇和一氧化碳代谢

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-04-14 DOI: 10.1111/1462-2920.70096

Nicolas A. Vecchini Santaella, Alfons J. M. Stams, Diana Z. Sousa

{"title":"Methanol and Carbon Monoxide Metabolism of the Thermophile Moorella caeni","authors":"Nicolas A. Vecchini Santaella, Alfons J. M. Stams, Diana Z. Sousa","doi":"10.1111/1462-2920.70096","DOIUrl":"https://doi.org/10.1111/1462-2920.70096","url":null,"abstract":"Moorella species are thermophilic acetogens that primarily produce acetate from one-carbon (C1) compounds including CO, CO2 (+H2), methanol and formate. Notably, Moorella caeni DSM 21394T displays a hydrogenogenic metabolism on CO and an acetogenic metabolism on methanol. Furthermore, M. caeni is unable to use CO2 (+H2) and grows only on formate in the presence of a methanogen or when thiosulfate is added as an electron acceptor. Presently, all theoretical frameworks for C1 metabolism in Moorella species are derived from experimental and genomic analyses of Moorella thermoacetica, which exhibits an acetogenic metabolism with all C1 substrates. In this study, we applied a transcriptomics approach to elucidate the mechanisms underlying the C1 metabolism of Moorella caeni during growth on methanol and CO. Our results indicate that respiratory Complex 1, a proton-translocating (ubi)quinone oxidoreductase, is the primary respiratory enzyme in methanol-grown cells of M. caeni. Conversely, in CO-grown cells, an energy-conserving hydrogenase complex (Ech) appears to be the primary respiratory complex, alongside respiratory Complex 1. This study provides insight into the C1 metabolism of M. caeni and reveals variations in gene syntenies related to C1 metabolism among the Moorella genus.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 4","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70096","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143830967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Needle in a Haystack: Culturing Plant-Beneficial Helotiales Lineages From Plant Roots 大海捞针从植物根部培养有益于植物的螺旋藻菌系

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-04-14 DOI: 10.1111/1462-2920.70082

Pauline Bruyant, Jeanne Doré, Laurent Vallon, Yvan Moënne-Loccoz, Juliana Almario

{"title":"Needle in a Haystack: Culturing Plant-Beneficial Helotiales Lineages From Plant Roots","authors":"Pauline Bruyant, Jeanne Doré, Laurent Vallon, Yvan Moënne-Loccoz, Juliana Almario","doi":"10.1111/1462-2920.70082","DOIUrl":"https://doi.org/10.1111/1462-2920.70082","url":null,"abstract":"Root-associated Helotiales fungi are increasingly recognised as beneficial fungal partners promoting plant growth under nutrient-limited conditions, particularly, in non-mycorrhizal hosts lacking the ancestral arbuscular mycorrhizal symbiosis. However, the ecology of these fungi is still cryptic as relatively few lineages have been successfully cultivated from roots for further study. Here, we attempted the mass isolation of root endophytic fungi to evaluate the recovery of known plant-beneficial Helotiales lineages using a tailored culture-based approach. We sampled six wild non-mycorrhizal species from the Brassicaceae, Caryophyllaceae, and Cyperaceae, growing in nutrient-limited alpine soils. We isolated 602 root endophytes and compared this culturable diversity with the one observed via fungal ITS2 metabarcoding. Metabarcoding revealed that Helotiales taxa dominated the fungal communities, with 43% of these detected taxa also represented in our collection. Accordingly, most root endophytes in our collection (53%) were Helotiales. These isolates, some with P solubilisation potential, belonged primarily to three Helotialean clades and were phylogenetically related to plant growth-promoting or mycorrhizal-like strains. This analysis highlights that the roots of alpine non-mycorrhizal plants harbour diverse plant-beneficial root-endophytic Helotiales, and the isolates obtained are a promising resource to explore the plant-beneficial mechanisms and ecological traits of these fungi.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 4","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70082","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143831067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Wine Fermentation as a Model System for Microbial Ecology and Evolution 葡萄酒发酵作为微生物生态学和进化的模式系统

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-04-13 DOI: 10.1111/1462-2920.70092

Ignacio Belda, Sergio Izquierdo-Gea, Belen Benitez-Dominguez, Javier Ruiz, Jean C. C. Vila

{"title":"Wine Fermentation as a Model System for Microbial Ecology and Evolution","authors":"Ignacio Belda, Sergio Izquierdo-Gea, Belen Benitez-Dominguez, Javier Ruiz, Jean C. C. Vila","doi":"10.1111/1462-2920.70092","DOIUrl":"https://doi.org/10.1111/1462-2920.70092","url":null,"abstract":"<div>\u0000 \u0000 In vitro microbial communities have proven to be invaluable model systems for studying ecological and evolutionary processes experimentally. However, it remains unclear whether quantitative insights obtained from these laboratory systems can be applied to complex communities assembling and evolving in their natural ecological context. To bridge the gap between the lab and the ‘real-world’, there is a need for laboratory model systems that better approximate natural and semi-natural ecosystems. Wine fermentation presents an ideal system for this purpose, balancing experimental tractability with rich ecological and evolutionary dynamics. In this perspective piece we outline the key features that make wine fermentation a fruitful model system for ecologists and evolutionary biologists. We highlight the diversity of environmentally mediated interactions that shape community dynamics during fermentation, the complex evolutionary history of wine microbial populations, and the opportunity to study the impact of complex ecologies on evolutionary dynamics. By integrating knowledge from both wine research and microbial ecology and evolution we aim to enhance understanding and foster collaboration between these fields.\u0000 </div>","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 4","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143826926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Honeybee Associate Galleria mellonella Can Acquire Arsenophonus apicola Through Oral and Parenteral Infection Routes 蜜蜂伴生mellongallia可通过口腔和肠外感染途径获得尖Arsenophonus

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-04-13 DOI: 10.1111/1462-2920.70088

Trefor Simmons, Pol Nadal-Jimenez, Gregory D. D. Hurst

{"title":"The Honeybee Associate Galleria mellonella Can Acquire Arsenophonus apicola Through Oral and Parenteral Infection Routes","authors":"Trefor Simmons, Pol Nadal-Jimenez, Gregory D. D. Hurst","doi":"10.1111/1462-2920.70088","DOIUrl":"https://doi.org/10.1111/1462-2920.70088","url":null,"abstract":"Members of the genus Arsenophonus are classically considered to be vertically transmitted endosymbiotic associates of invertebrates. Acquisition of Arsenophonus apicola by Apis mellifera honeybees through social and environmental pathways raises the possibility that this species can infect a broader range of host species. In this study, we tested whether a natural inhabitant of bee hives, the wax moth Galleria mellonella, was a suitable host for A. apicola. We first demonstrated A. apicola colonised G. mellonella larvae following injection at doses as low as 104 CFU. A similar capacity of A. apicola to infect G. mellonella orally was evidenced, impacting waxworm development and mortality. Microscopy indicated that A. apicola crossed from gut to hemocoel in the G. mellonella crop, inducing melanisation. PCR screening of Galleria individuals in an apiary sample confirmed exposure of Galleria in the hive context. We conclude that A. apicola is capable of infecting and damaging hive associates. These findings raise two onward avenues of research: first, to investigate whether A. apicola's presence could protect hives against Galleria infestations, and second, to utilise model insect G. mellonella for immunity research to uncover the interplay between A. apicola and insect host defences whilst elucidating virulence factors utilised by A. apicola during infection.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 4","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70088","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143826927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0