Environmental microbiology最新文献_第6页

Ring A Cleaving Beta-Diketone Hydrolase Is a Key Enzyme of Steroid Degradation in Anaerobic Bacteria

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-01-27 DOI: 10.1111/1462-2920.70034

Christian Jacoby, Lina Peller, Jana Wenzler, Monika Luttermann, Wolfgang Seiche, Bernhard Breit, Matthias Boll

{"title":"Ring A Cleaving Beta-Diketone Hydrolase Is a Key Enzyme of Steroid Degradation in Anaerobic Bacteria","authors":"Christian Jacoby, Lina Peller, Jana Wenzler, Monika Luttermann, Wolfgang Seiche, Bernhard Breit, Matthias Boll","doi":"10.1111/1462-2920.70034","DOIUrl":"10.1111/1462-2920.70034","url":null,"abstract":"Bacterial degradation of ubiquitous and persistent steroids such as steroid hormones is important for their removal from the environment. Initial studies of steroid degradation in anaerobic bacteria suggested that ring-cleaving hydrolases are involved in oxygen-independent sterane skeleton degradation. However, the enzymes involved in ring A cleavage of the common intermediate androsta-1,4-diene-3,17-dione have remained unknown. Here, we enriched a ring A hydrolase from cholesterol/nitrate grown Sterolibacterium denitrificans and from Escherichia coli after heterologous expression of its gene. This enzyme specifically cleaves the cyclic 1,3-diketone of the central degradation intermediate, androsta-1,3,17-trione to 1,17-dioxo-2,3-seco-androstan-3-oate (DSAO), a hallmark reaction of anaerobic steroid degradation. The highly conserved ring A hydrolase was identified in all known and many previously unknown steroid-degrading proteobacteria. Using enriched enzymes, we enzymatically produced DSAO from the chemically synthesised androsta-1-en-3,17-dione precursor, allowing the identification of subsequent metabolites involved in ring A degradation. The results obtained suggest the involvement of an additional hydrolase, an aldolase, and a β-oxidation-like cascade for complete ring A degradation to form the three-ring 5,10-seco-1,2,3,4-tetranorandrosta-5,17-dione. The results identified a key enzyme of anaerobic steroid degradation that may serve as a functional marker for monitoring steroid contaminant degradation at anoxic environmental sites.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70034","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143044201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characteristics of Phages and Their Interactions With Hosts in Anaerobic Reactors

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-01-27 DOI: 10.1111/1462-2920.70040

Yan Zeng, Xiaozhong Zhong, Yating Chen, Min Gou, Ke Yu, Yue-Qin Tang

{"title":"Characteristics of Phages and Their Interactions With Hosts in Anaerobic Reactors","authors":"Yan Zeng, Xiaozhong Zhong, Yating Chen, Min Gou, Ke Yu, Yue-Qin Tang","doi":"10.1111/1462-2920.70040","DOIUrl":"10.1111/1462-2920.70040","url":null,"abstract":"<div>\u0000 \u0000 Anaerobic digestion (AD) of organic wastes relies on the interaction and cooperation of various microorganisms. Phages are crucial components of the microbial community in AD systems, but their diversity and interactions with the prokaryotic populations are still inadequately comprehended. In this study, 2121 viral operational taxonomic units (vOTUs) were recovered from 12 anaerobic fatty acid–fed reactors. Notably, 63.1% of these vOTUs could not be assigned to any known family, revealing a substantial presence of uncharted phages specifically associated with AD environments. Over half of the vOTUs associated with hosts had the capability to infect multiple hosts, ranging from 2 to 49, with a prevalent tendency to infect 2–5 hosts. In silico predictions of phage-host linkages uncovered that only a small fraction of vOTUs were shared across different functional groups, including fermentative bacteria, syntrophic fatty acid–oxidising bacteria (SFOB) and methanogens. Phages linked to hosts in all three groups primarily consisted of generalists and temperate species, especially those linked to SFOB. Additionally, metabolic reconstruction identified auxiliary metabolic genes participating in fatty acid degradation, methanogenesis and energy conservation. The present study provides insights into phage characteristics and their in situ interactions with prokaryotic hosts, highlighting their ecological role in AD systems.\u0000 </div>","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143044200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antarctic Geothermal Soils Exhibit an Absence of Regional Habitat Generalist Microorganisms

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-01-27 DOI: 10.1111/1462-2920.70032

Stephen E. Noell, Jaber Abbaszadeh, Huw Richards, Marie Labat Saint Vincent, Charles K. Lee, Craig W. Herbold, Matthew B. Stott, S. Craig Cary, Ian R. McDonald

{"title":"Antarctic Geothermal Soils Exhibit an Absence of Regional Habitat Generalist Microorganisms","authors":"Stephen E. Noell, Jaber Abbaszadeh, Huw Richards, Marie Labat Saint Vincent, Charles K. Lee, Craig W. Herbold, Matthew B. Stott, S. Craig Cary, Ian R. McDonald","doi":"10.1111/1462-2920.70032","DOIUrl":"10.1111/1462-2920.70032","url":null,"abstract":"Active geothermal systems are relatively rare in Antarctica and represent metaphorical islands ideal to study microbial dispersal. In this study, we tested the macro-ecological concept that high dispersal rates result in communities being dominated by either habitat generalists or specialists by investigating the microbial communities on four geographically separated geothermal sites on three Antarctic volcanoes (Mts. Erebus, Melbourne, and Rittman). We found that the microbial communities at higher temperature (max 65°C) sites (Tramway Ridge on Erebus and Rittmann) were unique from each other and were dominated by a variety of novel Archaea from class Nitrososphaeria, while lower temperature (max 50°C) sites (Western Crater on Erebus and Melbourne) had characteristically mesophilic communities (Planctomycetes, Acidobacteriota, etc.) that were highly similar. We found that 97% of the detected microbial taxa were regional habitat specialists, with no generalists, with community assembly driven by high dispersal rates and drift (25% and 30% of community assembly, respectively), not environmental selection. Our results indicate that for microbial communities experiencing high dispersal rates between isolated communities, habitat specialists may tend to out-compete habitat generalists.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70032","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143050867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evolutionary Novelties in Bacteria and the Missing Backdrop of the Environment

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-01-27 DOI: 10.1111/1462-2920.70044

Shraddha Karve

{"title":"Evolutionary Novelties in Bacteria and the Missing Backdrop of the Environment","authors":"Shraddha Karve","doi":"10.1111/1462-2920.70044","DOIUrl":"10.1111/1462-2920.70044","url":null,"abstract":"<div>\u0000 \u0000 Evolutionary novelty has been one of the central themes in the field of evolutionary biology for many years. Structural and functional innovations such as scales in the reptiles, fins in the fishes and mammary glands in the mammals have been the focus of the studies. Insights obtained from these studies have shaped the criterion for the identification of novelty as well as provide the framework for studying novelty. In this article, I argue that unicellular organisms present an excellent opportunity for the investigation of evolutionary novelty. Even though bacteria share some fundamental aspects of novelty with higher organisms, there are definite departures. Here, I outline these departures in four different contexts: criterion for the identification of novelty, types of evolutionary novelties, level of biological complexity that bacteria embody and, most importantly, the role of the environment. Identifying the role of the environment allows the categorisation of novelty as probable or improbable and adaptive or latent. This categorisation of novel traits, based on the role of the environment, can facilitate the study of novelty in bacteria. Insights obtained from such studies are crucial for understanding the fundamental aspects of evolutionary novelty.\u0000 </div>","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143044202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulation of Rhizobial Nodulation Genes by Flavonoid-Independent NodD Supports Nitrogen-Fixing Symbioses With Legumes

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-01-25 DOI: 10.1111/1462-2920.70014

Timothy L. Haskett, Louise Cooke, Patrick Green, Philip S. Poole

{"title":"Regulation of Rhizobial Nodulation Genes by Flavonoid-Independent NodD Supports Nitrogen-Fixing Symbioses With Legumes","authors":"Timothy L. Haskett, Louise Cooke, Patrick Green, Philip S. Poole","doi":"10.1111/1462-2920.70014","DOIUrl":"10.1111/1462-2920.70014","url":null,"abstract":"Rhizobia and legumes form a symbiotic relationship resulting in the formation of root structures known as nodules, where bacteria fix nitrogen. Legumes release flavonoids that are detected by the rhizobial nodulation (Nod) protein NodD, initiating the transcriptional activation of nod genes and subsequent synthesis of Nod Factors (NFs). NFs then induce various legume responses essential for this symbiosis. Although evidence suggests differential regulation of nodD expression and NF biosynthesis during symbiosis, the necessity of this regulation for the formation of nitrogen-fixing nodules remains uncertain. Here, we demonstrate that deletion of the Rlv3841 NodD regulatory domain results in a constitutively active protein (NodDFI) capable of activating NF biosynthesis gene expression without the presence of flavonoids. Optimised constitutive expression of nodDFI or nodD3 in nodD null mutants led to wild-type levels of nodulation and nitrogen fixation in pea and M. truncatula, respectively, indicating that flavonoid-regulated nodD expression is not essential for supporting symbiosis. These findings illustrate that transcriptional control of flavonoid-independent NodD regulators can be employed to drive NF biosynthesis, which holds potential for engineering symbiosis between rhizobia and cereals equipped with reconstituted NF receptors.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70014","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143030936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heat-Evolved Microalgae (Symbiodiniaceae) Are Stable Symbionts and Influence Thermal Tolerance of the Sea Anemone Exaiptasia diaphana 热演化微藻（共生藻科）是稳定的共生体并影响海葵耐热性

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-01-21 DOI: 10.1111/1462-2920.70011

Wing Yan Chan, Rumi Sakamoto, Talisa Doering, Vinod K. Narayana, David P. De Souza, Malcolm J. McConville, Madeleine J. H. van Oppen

{"title":"Heat-Evolved Microalgae (Symbiodiniaceae) Are Stable Symbionts and Influence Thermal Tolerance of the Sea Anemone Exaiptasia diaphana","authors":"Wing Yan Chan, Rumi Sakamoto, Talisa Doering, Vinod K. Narayana, David P. De Souza, Malcolm J. McConville, Madeleine J. H. van Oppen","doi":"10.1111/1462-2920.70011","DOIUrl":"10.1111/1462-2920.70011","url":null,"abstract":"Symbiotic cnidarians, such as sea anemones and corals, rely on their mutualistic microalgal partners (Symbiodiniaceae) for survival. Marine heatwaves can disrupt this partnership, and it has been proposed that introducing experimentally evolved, heat-tolerant algal symbionts could enhance host thermotolerance. To test this hypothesis, the sea anemone Exaiptasia diaphana (a coral model) was inoculated with either the heterologous wild type or heat-evolved algal symbiont, Cladocopium proliferum, and homologous wild-type Breviolum minutum. The novel symbioses persisted for 1.5 years and determined holobiont thermotolerance during a simulated summer heatwave. Anemones hosting SS8, one of the six heat-evolved strains tested, exhibited the highest thermotolerance. Notably, anemones hosting the wild-type C. proliferum (WT10) were the second most thermally tolerant group, whereas anemones hosting the heat-evolved SS5 or SS9 strains were among the most thermosensitive. Elevated temperatures led to an increase in the levels of many amino acids and a decrease in tricarboxylic acid (TCA) metabolites in all anemone hosts, potentially indicating an increase in autophagy and a reduction in energy and storage production. Some consistent differences were observed in changes in metabolite levels between anemone groups in response to elevated temperature, suggesting that the algal symbiont influenced host metabolome and nutritional budget.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70011","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142991912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Functional and Structural Succession of Mesic-Grassland Soil Microbiomes Beneath Decomposing Large Herbivore Carcasses 大型草食动物尸体分解下Mesic -草地土壤微生物群的功能和结构演替

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-01-17 DOI: 10.1111/1462-2920.70022

Jacques Fouché, Pedro H. Lebre, Haemish A. Melville, Don A. Cowan

{"title":"The Functional and Structural Succession of Mesic-Grassland Soil Microbiomes Beneath Decomposing Large Herbivore Carcasses","authors":"Jacques Fouché, Pedro H. Lebre, Haemish A. Melville, Don A. Cowan","doi":"10.1111/1462-2920.70022","DOIUrl":"10.1111/1462-2920.70022","url":null,"abstract":"Plant detritus is abundant in grasslands but decomposes slowly and is relatively nutrient-poor, whereas animal carcasses are labile and nutrient-rich. Recent studies have demonstrated that labile nutrients from carcasses can significantly alter the long-term soil microbial function at an ecosystem scale. However, there is a paucity of knowledge on the functional and structural response and temporal scale of soil microbiomes beneath large herbivore carcasses. This study compared microbiome functions and structures of soil beneath Connochaetes taurinus (hereafter ‘wildebeest’) carcasses at various postmortem intervals of decomposition to matched control samples over 18 months. Microbial functions were compared by their community-level physiological profiles determined by sole-carbon substrate utilisation and structures by metagenomic sequences using 16S rRNA gene markers. Overall metabolism and metabolic diversity remained increased and functionally dissimilar to control soils throughout the experimental period, with successive sole-carbon substrate utilisation observed. Conversely, diversity was initially reduced and structurally dissimilar from the control soil but recovered within the experimental period. The study contributes to the knowledge of carcass decomposition by investigating the long-term soil microbiome dynamics resulting from large herbivore carcasses decomposing in a mesic grassland. Microbial functional succession and ecologically relevant bacterial biomarkers of soil beneath the decomposing carcasses were identified for various postmortem intervals.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70022","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142988499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid Icm/Dot T4SS Inactivation Prevents Resuscitation of Heat-Induced VBNC Legionella pneumophila by Amoebae 快速Icm/Dot T4SS失活阻止阿米巴热诱导的VBNC嗜肺军团菌复苏

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-01-14 DOI: 10.1111/1462-2920.70035

Camille Schmid, Hubert Hilbi

{"title":"Rapid Icm/Dot T4SS Inactivation Prevents Resuscitation of Heat-Induced VBNC Legionella pneumophila by Amoebae","authors":"Camille Schmid, Hubert Hilbi","doi":"10.1111/1462-2920.70035","DOIUrl":"10.1111/1462-2920.70035","url":null,"abstract":"<div>\u0000 \u0000 Legionella pneumophila, the causative agent of Legionnaires' disease, employs the Icm/Dot Type IV secretion system (T4SS) to replicate in amoebae and macrophages. The opportunistic pathogen responds to stress by forming ‘viable but non-culturable’ (VBNC) cells, which cannot be detected by standard cultivation-based techniques. In this study, we document that L. pneumophila enters the VBNC state after exposure to heat stress at 50°C for 30 h, at 55°C for 5 h or at 60°C for 30 min, while still retaining metabolic activity and intact cell membranes. Resuscitation of heat-induced VBNC L. pneumophila neither occurred in amoebae nor in macrophages. VBNC L. pneumophila showed impaired uptake by phagocytes, formation of Legionella-containing vacuoles (LCVs), and Icm/Dot-dependent secretion of effector proteins. The T4SS was rapidly inactivated already upon exposure to 50°C for 3–5 h, while the bacteria were still culturable. The Legionella quorum sensing (Lqs)-LvbR network is implicated in VBNC induction, since the ∆lvbR and ∆lqsR mutant strains showed a more pronounced heat sensitivity than the parental strain, and the ∆lqsA mutant was less heat sensitive. Taken together, our results reveal that heat exposure of L. pneumophila rapidly inactivates the Icm/Dot T4SS before the VBNC state is induced, thus impairing resuscitation by amoebae.\u0000 </div>","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142981894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assembly Graph as the Rosetta Stone of Ecological Assembly 组装图是生态组装的罗塞塔石碑

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-01-13 DOI: 10.1111/1462-2920.70030

Chuliang Song

{"title":"Assembly Graph as the Rosetta Stone of Ecological Assembly","authors":"Chuliang Song","doi":"10.1111/1462-2920.70030","DOIUrl":"10.1111/1462-2920.70030","url":null,"abstract":"<div>\u0000 \u0000 Ecological assembly—the process of ecological community formation through species introductions—has recently seen exciting theoretical advancements across dynamical, informational, and probabilistic approaches. However, these theories often remain inaccessible to non-theoreticians, and they lack a unifying lens. Here, I introduce the assembly graph as an integrative tool to connect these emerging theories. The assembly graph visually represents assembly dynamics, where nodes symbolise species combinations and edges represent transitions driven by species introductions. Through the lens of assembly graphs, I review how ecological processes reduce uncertainty in random species arrivals (informational approach), identify graphical properties that guarantee species coexistence and examine how the class of dynamical models constrain the topology of assembly graphs (dynamical approach), and quantify transition probabilities with incomplete information (probabilistic approach). To facilitate empirical testing, I also review methods to decompose complex assembly graphs into smaller, measurable components, as well as computational tools for deriving empirical assembly graphs. In sum, this math-light review of theoretical progress aims to catalyse empirical research towards a predictive understanding of ecological assembly.\u0000 </div>","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142975475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hepatincolaceae (Alphaproteobacteria) are Distinct From Holosporales and Independently Evolved to Associate With Ecdysozoa 肝菌科（阿尔法变形菌门）与全孢子菌门不同，并独立进化为与外翻虫门相关

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-01-10 DOI: 10.1111/1462-2920.70028

Michele Castelli, Leandro Gammuto, Diona Podushkina, Matteo Vecchi, Tiziana Altiero, Emanuela Clementi, Roberto Guidetti, Lorena Rebecchi, Davide Sassera

{"title":"Hepatincolaceae (Alphaproteobacteria) are Distinct From Holosporales and Independently Evolved to Associate With Ecdysozoa","authors":"Michele Castelli, Leandro Gammuto, Diona Podushkina, Matteo Vecchi, Tiziana Altiero, Emanuela Clementi, Roberto Guidetti, Lorena Rebecchi, Davide Sassera","doi":"10.1111/1462-2920.70028","DOIUrl":"10.1111/1462-2920.70028","url":null,"abstract":"The Hepatincolaceae (Alphaproteobacteria) are a group of bacteria that inhabit the gut of arthropods and other ecdysozoans, associating extracellularly with microvilli. Previous phylogenetic studies, primarily single-gene analyses, suggested their relationship to the Holosporales, which includes intracellular bacteria in protist hosts. However, the genomics of Hepatincolaceae is still in its early stages. In this study, the number of available Hepatincolaceae genomes was increased to examine their evolutionary and functional characteristics. It was found that the previous phylogenetic grouping with Holosporales was incorrect due to sequence compositional biases and that Hepatincolaceae form an independent branch within the Hepatincolaceae. This led to a reinterpretation of their features, proposing a new evolutionary scenario that involves an independent adaptation to host association compared to the Holosporales, with distinct specificities. The Hepatincolaceae exhibit greater nutritional flexibility, utilising various molecules available in the host gut and thriving in anaerobic conditions. However, they have a less complex mechanism for modulating host interactions, which are likely less direct than those of intracellular bacteria. In addition, representatives of Hepatincolaceae show several lineage-specific traits related to differences in host species and life conditions.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70028","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142961838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0