Environmental microbiology最新文献_第6页

Promotion of Fungal Bioactivity by Extracellular Polymeric Substances Under Phosphorus Deficiency 缺磷条件下胞外聚合物质对真菌生物活性的促进作用

IF 4 2区生物学

Environmental microbiology Pub Date : 2025-07-08 DOI: 10.1111/1462-2920.70147

Shang Pan, Yizhou Jiang, Shihui Yan, Meiyue Xu, Yuyang Cao, Haoyang Weng, Zhijun Wang, Yixiao Shi, Zhen Li

{"title":"Promotion of Fungal Bioactivity by Extracellular Polymeric Substances Under Phosphorus Deficiency","authors":"Shang Pan, Yizhou Jiang, Shihui Yan, Meiyue Xu, Yuyang Cao, Haoyang Weng, Zhijun Wang, Yixiao Shi, Zhen Li","doi":"10.1111/1462-2920.70147","DOIUrl":"10.1111/1462-2920.70147","url":null,"abstract":"<div>\u0000 \u0000 Extracellular polymeric substances (EPS) can work as a highly efficient nutrient source for many microorganisms. This study investigated the influences of EPS on the bioactivities of a typical phosphate-solubilising fungus, that is Aspergillus niger. The EPS were extracted from Rhodotorula mucilaginosa, with the addition of varying levels of inorganic phosphates. Under phosphorus (P) limitation, the cell wall thickness of A. niger increased from ~0.2 μm to ~0.4 μm. The addition of EPS relieved this phenomenon under the stress. In addition, it showed that EPS significantly promoted microbial growth with a four-fold increase in microbial biomass. For the treatment with the addition of sole inorganic phosphate, A. niger primarily secreted oxalic acid for phosphate solubilisation. However, for the treatment with sole EPS, the secretion of oxalic acid was inhibited, while the activity of acid phosphatase was promoted. Moreover, the concentration of oxalic acid was significantly reduced from approximately 90 to 1 mg/L during the co-existence of inorganic phosphate and EPS. Meanwhile, the activity of acid phosphatase was also enhanced. Therefore, A. niger can balance its strategy of P solubilisation, depending on the types of P sources. Furthermore, EPS was preferentially utilised over inorganic phosphate. Our findings highlight the potential of EPS for sustainable P supply.\u0000 </div>","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 7","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144582271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Eastern Joshua Tree Arbuscular Mycorrhizal Fungi Largely Consistent Across Roots, Soils, and Seasons 东部约书亚树丛枝菌根真菌在根、土壤和季节上基本一致

IF 4 2区生物学

Environmental microbiology Pub Date : 2025-07-07 DOI: 10.1111/1462-2920.70138

Arik Joukhajian, Sydney I. Glassman

{"title":"Eastern Joshua Tree Arbuscular Mycorrhizal Fungi Largely Consistent Across Roots, Soils, and Seasons","authors":"Arik Joukhajian, Sydney I. Glassman","doi":"10.1111/1462-2920.70138","DOIUrl":"10.1111/1462-2920.70138","url":null,"abstract":"The Mojave Desert is home to iconic Joshua trees threatened by climate change. Most desert plants form mutually beneficial partnerships with arbuscular mycorrhizal fungi (AMF), yet the AMF of the Eastern Joshua tree (Yucca jaegeriana) remain completely uncharacterized. We tested how Y. jaegeriana AMF spore abundance, richness, and composition varied when sampling 20 trees across 4 seasons from roots versus soils. We confirmed root colonisation via staining, assessed spore abundance via microscopy, and used Illumina MiSeq to sequence AMF virtual taxa (VT) with WANDA AML2 primers. We identified 12 spore morphotypes and 47 VTs across 5 families within Glomeromycotina, and the most abundant VT, Glomus VTX00294, appeared in 87% of soil and root samples. The majority of VTs (26/47) were present across all seasons and were shared among soil and roots (38/47), with more VTs unique to soil. In soil, per tree mean spore abundance and AMF richness were lowest in Summer but consistent across other seasons, with richness ranging from 8.8 to 11.5 VTs and mean root richness consistent across seasons. We conclude that sampling from soils rather than roots and any season other than Summer will yield the most diverse AMF communities.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 7","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70138","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144573767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extracellular Vesicles of a Phytobeneficial Bacterium Trigger Distinct Systemic Response in Plant 植物有益菌胞外囊泡在植物中引发不同的系统反应

IF 4 2区生物学

Environmental microbiology Pub Date : 2025-07-07 DOI: 10.1111/1462-2920.70141

Timothée Zannis-Peyrot, Lucas Degusseau, Pierre-Yves Dugas, Fabiola Bastian, Matthieu Gaucher, Vincent Gaillard, Gilles Comte, Florence Wisniewski-Dye, Isabelle Kerzaon, Céline Lavire, Ludovic Vial

{"title":"Extracellular Vesicles of a Phytobeneficial Bacterium Trigger Distinct Systemic Response in Plant","authors":"Timothée Zannis-Peyrot, Lucas Degusseau, Pierre-Yves Dugas, Fabiola Bastian, Matthieu Gaucher, Vincent Gaillard, Gilles Comte, Florence Wisniewski-Dye, Isabelle Kerzaon, Céline Lavire, Ludovic Vial","doi":"10.1111/1462-2920.70141","DOIUrl":"10.1111/1462-2920.70141","url":null,"abstract":"<div>\u0000 \u0000 Bacterial extracellular vesicles (EVs) are lipidic shuttles involved in inter-species communication, virulence, and host immune modulation. While their roles are increasingly understood in animal-bacteria interactions, knowledge of phytobacterial EVs remains limited. Recent findings indicate that biotic factors like hydroxycinnamic acids can regulate EV production. Hydroxycinnamic acids, such as ferulic acid, are abundant lignin components in the plant environment, influencing the ecology of numerous phytobacteria. Azospirillum sp. B510, a phytobeneficial bacterium, induces the accumulation of hydroxycinnamic acid derivatives in plants and can metabolise them. This study hypothesised that ferulic acid in the Azospirillum sp. B510 environment would influence EV production and, conversely, that these EVs would impact plant metabolites and defence gene expression. Our results demonstrate that ferulic acid influences the content of EVs released by Azospirillum sp. B510. Furthermore, bacterial EVs impact plant physiology at a systemic level according to their cargo. EVs induce systemic changes in Solanum lycopersicum metabolome, including alterations in hydroxycinnamic acid amide derivatives in roots and steroidal alkaloids in aerial tissues. Bacterial EVs also modulate tomato defence gene expression correlating with observed metabolite accumulation patterns. This research provides evidence of a global effect of bacterial EVs, highlighting the dynamic nature of plant-bacteria interactions mediated by EVs.\u0000 </div>","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 7","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144573768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High Prevalence of Plasmid-Mediated Quinolone Resistance in Salmonella enterica Serovars Isolated From Surface Water 地表水分离的肠沙门氏菌血清型质粒介导的喹诺酮类耐药性高流行率

IF 4 2区生物学

Environmental microbiology Pub Date : 2025-07-07 DOI: 10.1111/1462-2920.70140

Daniel F. M. Monte, Alan Douglas de Lima Rocha, Mateus Lacerda Pereira Lemos, Laiorayne Araújo de Lima, Julia Memrava Cabrera, Nádyra Jerônimo da Silva, Xinyang Huang, Zhao Chen, Eric W. Brown, Marc W. Allard, Rebecca L. Bell, Magaly Toro, Jianghong Meng, Celso José Bruno de Oliveira

{"title":"High Prevalence of Plasmid-Mediated Quinolone Resistance in Salmonella enterica Serovars Isolated From Surface Water","authors":"Daniel F. M. Monte, Alan Douglas de Lima Rocha, Mateus Lacerda Pereira Lemos, Laiorayne Araújo de Lima, Julia Memrava Cabrera, Nádyra Jerônimo da Silva, Xinyang Huang, Zhao Chen, Eric W. Brown, Marc W. Allard, Rebecca L. Bell, Magaly Toro, Jianghong Meng, Celso José Bruno de Oliveira","doi":"10.1111/1462-2920.70140","DOIUrl":"10.1111/1462-2920.70140","url":null,"abstract":"Considering the increasing reports of Salmonella enterica strains resistant to quinolones, antimicrobials frequently employed as therapeutic agents globally, our goal was to investigate the occurrence of plasmid-mediated quinolone resistance (PMQR) determinants in S. enterica recovered from natural surface waters in Paraíba state, Brazil. Water samples (n = 230) were collected monthly in triplicate using modified Moore swabs from 29 sampling sites belonging to 10 large dams. After conventional microbial isolation, representative isolates (n = 938) were submitted to whole genome sequencing, assembly and annotation. Antimicrobial resistance genes (ARGs) were identified, and core genome multilocus sequence typing (cgMLST) was used to infer phylogenetic relationships. Among recovered S. enterica, 130 (13.9%) isolates harboured PMQR determinants; 124 (95.4%) harboured qnrB19, while 6 (4.6%) harboured qnrS1. Multiple other ARGs associated with resistance to aminoglycosides, β-lactams, sulphonamides, tetracyclines and fosfomycin were identified. The diversity of ARGs and plasmids suggests a highly complex resistance landscape. Phylogenetic analysis revealed clustering by serovar and sequence type but not by resistance profile or geographic origin. The absence of association between phylogeny and ARGs highlights the potential role of horizontal gene transfer in disseminating resistance genes in water. Our findings reinforce the importance of antimicrobial resistance surveillance in surface waters.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 7","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70140","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144573769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structure and Function Features of Abundant and Rare Prokaryotic Communities Along Nearshore to Offshore Transitions 近岸向近海过渡中丰富和稀有原核生物群落的结构和功能特征

IF 4 2区生物学

Environmental microbiology Pub Date : 2025-07-07 DOI: 10.1111/1462-2920.70144

Mengjie Wu, Tingrui Zhang, Shuo Han, Shengxin Huan, Yuelu Jiang, Yong Wang, Zhonghua Cai, Jin Zhou

{"title":"Structure and Function Features of Abundant and Rare Prokaryotic Communities Along Nearshore to Offshore Transitions","authors":"Mengjie Wu, Tingrui Zhang, Shuo Han, Shengxin Huan, Yuelu Jiang, Yong Wang, Zhonghua Cai, Jin Zhou","doi":"10.1111/1462-2920.70144","DOIUrl":"10.1111/1462-2920.70144","url":null,"abstract":"<div>\u0000 \u0000 Abundant and rare taxa are crucial members of the marine microbial community. However, their biodiversity, assembly mechanisms, functional characteristics and ecological response strategies remain poorly understood. In this study, 16S rRNA and metagenomic sequencing were carried out to reveal the structural and functional features of abundant and rare taxa across the transition from nearshore to offshore. The results showed that the biodiversity of both abundant and rare taxa decreased with increasing distance from shore, with rare taxa exhibiting relatively higher diversity indices than abundant ones. Neutral model analysis revealed that the assembly process gradually changed from deterministic to stochastic from nearshore to offshore among abundant taxa. In contrast, among rare taxa, a stochastic process dominated nearshore, whereas a deterministic process was predominant in the offshore environment. Meanwhile, the proportion of variance that could be explained by environmental factors was relatively higher among abundant communities than among rare ones. A co-occurrence network analysis indicated that rare communities displayed greater complexity and a higher degree of modularity than abundant communities. Functionally, abundant communities tended to favour an r-strategy, whereas rare communities leaned towards a K-strategy. Our results strengthen the understanding of the ecological mechanisms controlling microbial community patterns along coastal-to-open water transitions.\u0000 </div>","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 7","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144573766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Successful Transmission and Isolation of a Fungal Pathogen From Wild Frogs to a Captive Amphibian Model Species: Fine Scale Pathogen Genetic Diversity and Infection-Induced Changes in Skin Bacteria 一种真菌病原体从野生青蛙到圈养两栖动物模型物种的成功传播和分离：细尺度病原体遗传多样性和感染诱导皮肤细菌的变化

IF 4 2区生物学

Environmental microbiology Pub Date : 2025-07-03 DOI: 10.1111/1462-2920.70136

Tamilie Carvalho, Daniel Medina, Timothy Y. James

{"title":"Successful Transmission and Isolation of a Fungal Pathogen From Wild Frogs to a Captive Amphibian Model Species: Fine Scale Pathogen Genetic Diversity and Infection-Induced Changes in Skin Bacteria","authors":"Tamilie Carvalho, Daniel Medina, Timothy Y. James","doi":"10.1111/1462-2920.70136","DOIUrl":"10.1111/1462-2920.70136","url":null,"abstract":"Amphibian populations worldwide are severely threatened, in part, by the pathogenic fungus Batrachochytrium dendrobatidis (Bd). While Bd has driven many amphibian declines and extinctions, its impact varies, with some populations exhibiting resistance or tolerance. Understanding the mechanisms behind this variation, together with Bd genetic diversity, is crucial for conservation. We used the model organism Hymenochirus boettgeri in a lab-based transmission experiment designed to isolate Bd derived from wild amphibian hosts with low pathogen loads. Through successful transmission and subsequent isolation and genotyping, we identified multiple Bd genotypes from a single population, including a potential sexual recombinant, all belonging to the Global Panzootic Lineage (Bd-GPL). This finding contributes to evidence of ongoing genetic mixing of Bd in natural environments. Additionally, we leveraged this experiment to assess Bd-induced changes in amphibian skin bacteria. Our results showed significant changes in the skin bacterial communities of H. boettgeri after acquiring Bd infection, including reductions in bacterial diversity, and shifts in community composition, as observed in others susceptible species. These findings highlight the value of transmission experiments in isolating Bd from wild hosts and underscore the potential of H. boettgeri as a model to investigate Bd genetic diversity and host-associated microbiome responses to infection.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 7","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70136","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144536910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deterioration of Masonry Cultural Relics by Microbes: Current Remediation Strategies and Prospects 微生物对砖石文物破坏的修复策略与展望

IF 4 2区生物学

Environmental microbiology Pub Date : 2025-07-03 DOI: 10.1111/1462-2920.70142

Sheng Xiong, Yuzhe Xu, Ji Zhou

{"title":"Deterioration of Masonry Cultural Relics by Microbes: Current Remediation Strategies and Prospects","authors":"Sheng Xiong, Yuzhe Xu, Ji Zhou","doi":"10.1111/1462-2920.70142","DOIUrl":"10.1111/1462-2920.70142","url":null,"abstract":"We review the mechanism of microbial deterioration of masonry cultural relics and their protective strategies. Firstly, the biogeochemical and biogeophysical mechanisms of microbial deterioration of cultural relics are described, including microbial colonisation, biofilm formation, inorganic and organic acid secretion and salt crystallisation. Then, this work introduces a variety of microbial deterioration prevention strategies, including biological control, chemical protection, physical and mechanical removal technology and the application of advanced materials. Finally, we discuss the application potential of advanced materials, such as nano-antibacterial materials and functional polymer materials in the protection of cultural relics, which can not only effectively isolate the colonisation of external microbes but also enhance the contamination resistance of cultural relics surfaces. Future studies should further explore the specific mechanism of microbial deterioration on cultural relics of different materials and combine protection technology with environmental factors to achieve long-term effective protection of cultural relics.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 7","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70142","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144536908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sediments From a Seasonally Euxinic Coastal Ecosystem Show High Nitrogen Cycling Potential 季节性富氧海岸生态系统沉积物显示出高氮循环潜力

IF 4 2区生物学

Environmental microbiology Pub Date : 2025-07-02 DOI: 10.1111/1462-2920.70139

Isabel M. L. Rigutto, Ştefania C. Sburlan, Lars W. P. de Bont, Tom Berben, Rob M. de Graaf, Caroline P. Slomp, Mike S. M. Jetten

{"title":"Sediments From a Seasonally Euxinic Coastal Ecosystem Show High Nitrogen Cycling Potential","authors":"Isabel M. L. Rigutto, Ştefania C. Sburlan, Lars W. P. de Bont, Tom Berben, Rob M. de Graaf, Caroline P. Slomp, Mike S. M. Jetten","doi":"10.1111/1462-2920.70139","DOIUrl":"10.1111/1462-2920.70139","url":null,"abstract":"Coastal ecosystems are susceptible to eutrophication and deoxygenation, which may alter their nitrogen cycle dynamics. Here, we investigated the microbial nitrogen cycling potential in the sediment of a seasonally euxinic coastal ecosystem (Lake Grevelingen, NL) in winter and summer. Activity tests revealed ammonium (NH4+) oxidation potential with maximum potential rates up to 53 μmol g−1 day−1, even in anoxic sediment layers. A nitrifying microbial community was present in both oxic and anoxic sediment sections (up to 1.4% relative abundance). Nitrate (NO3−), nitrite (NO2−), and nitrous oxide (N2O) reduction potential were prominent across all sediment sections, with the highest potential rates (167 μmol NO3−∙g−1 day−1) in the surface sediment in summer. Denitrification (79.3%–98.4%) and dissimilatory nitrate reduction to ammonium (DNRA; 1.6%–20.7%) were the major NO3− removal pathways, as supported by the detection of the narG/napA, nirK/nirS, norB, nosZ and nrfA/otr genes in all sediment sections. The DNRA contribution increased with depth and with the addition of electron donors, such as monomethylamine. Anaerobic ammonium oxidation (anammox) was not detected in these eutrophic sediments. Combined, our results show that there is high potential for nitrogen removal in eutrophic coastal ecosystems, which may help further restoration measures.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 7","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70139","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144524579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Insight Into the Hydrolysis of Phthalate Esters by a Family IV Esterase 一类酯酶水解邻苯二甲酸酯的分子研究

IF 4 2区生物学

Environmental microbiology Pub Date : 2025-07-02 DOI: 10.1111/1462-2920.70134

Zhen Rong, Li-Guo Hong, Ying-Yi Huo, Jixi Li, Dao-Qiong Zheng, Yang Ha, Jeffrey Fan, Xue-Wei Xu, Yue-Hong Wu

{"title":"Molecular Insight Into the Hydrolysis of Phthalate Esters by a Family IV Esterase","authors":"Zhen Rong, Li-Guo Hong, Ying-Yi Huo, Jixi Li, Dao-Qiong Zheng, Yang Ha, Jeffrey Fan, Xue-Wei Xu, Yue-Hong Wu","doi":"10.1111/1462-2920.70134","DOIUrl":"10.1111/1462-2920.70134","url":null,"abstract":"<div>\u0000 \u0000 Phthalate esters (PAEs) are prevalent environmental contaminants, with their biodegradation efficiently driven by microorganisms through ester bond hydrolysis. This study investigates the mechanism of Poc14, a novel family IV esterase, using x-ray crystallography, bioinformatics, biochemistry and site-directed mutagenesis. Phylogenetic analysis classifies Poc14 as a family IV esterase with conserved catalytic motifs crucial for its activity. Poc14 retains over 80% activity at 50°C for 4 h and tolerates up to 5% methanol or DMF, though surfactants like Tweens inhibit its function. Poc14 activity is independent of metal ions, and the addition of EDTA further enhances its activity by approximately 130%. The 1.8 Å crystal structure reveals a CAP domain and two substrate channels. Enzyme assays show Poc14 hydrolyses short-chain diethyl phthalate (DEP) (Km = 0.068 mM, Vmax = 9975 μM/min/mg) but not long-chain di(2-ethylhexyl) phthalate (DEHP) due to steric hindrance. Molecular docking assessed Poc14's potential to hydrolyse DEP and DEHP after residue mutations, resulting in the Poc14-AAG variant. Poc14-AAG could hydrolyse one bond of DEHP and diester bonds of DEP. Our study positions Poc14 as a promising enzyme for environmental remediation, with potential for optimising DEHP degradation and exploring dimerisation effects.\u0000 </div>","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 7","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144524582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Skin Bacteriome Structure and Batrachochytrium dendrobatidis Prevalence Differs Amongst Two Sympatric Salamanders in the San Francisco Bay Area 旧金山湾区两种同域蝾螈皮肤菌群结构和树突壶菌流行率的差异

IF 4 2区生物学

Environmental microbiology Pub Date : 2025-07-02 DOI: 10.1111/1462-2920.70131

Aria Norwood, Jadyn Jamora, Micuel Madison, Jamiee Nguyen, Azan Yousaf, Katya Morales, Emily Vu, Obed Hernández-Gómez

{"title":"Skin Bacteriome Structure and Batrachochytrium dendrobatidis Prevalence Differs Amongst Two Sympatric Salamanders in the San Francisco Bay Area","authors":"Aria Norwood, Jadyn Jamora, Micuel Madison, Jamiee Nguyen, Azan Yousaf, Katya Morales, Emily Vu, Obed Hernández-Gómez","doi":"10.1111/1462-2920.70131","DOIUrl":"10.1111/1462-2920.70131","url":null,"abstract":"<div>\u0000 \u0000 Microbial surveys are becoming an important component of wildlife health research, especially in cases where environmental change and infectious diseases are serious issues. Amongst amphibians, Batrachochytrium spp. have the potential to be fatal pathogens that can impact the population health of numerous species. We assessed the skin bacteriome, prevalence of Batrachochytrium dendrobatidis (Bd) and body conditions of two salamander genera with different life histories: the California slender salamander (Batrachoseps attenuatus) and members of the Pacific newt species complex (Taricha torosa and Taricha granulosa; Taricha sp.). We used 16S rRNA V4 amplicon sequencing to characterise the bacterial communities. Taricha sp. had higher prevalence and loads of Bd compared to B. attenuatus, although the prevalence of Bd was restricted to two of the four regions tested. Both salamander types possessed similar bacterial community richness, and the composition of the skin bacteriome varied mostly by region. However, we did find certain associations between the salamander genera and certain ASVs, with mostly members of the family Burkholderiaceae driving the difference. Our results provide additional evidence for the presence of structure in Bd prevalence and bacteriome composition amongst sympatric amphibians that inhabit different microhabitats.\u0000 </div>","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 7","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144524581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0