Environmental microbiology最新文献_第10页

Defence systems and horizontal gene transfer in bacteria 细菌的防御系统和水平基因转移

IF 5.1 2区生物学

Environmental microbiology Pub Date : 2024-04-21 DOI: 10.1111/1462-2920.16630

Roman Kogay, Yuri I. Wolf, Eugene V. Koonin

{"title":"Defence systems and horizontal gene transfer in bacteria","authors":"Roman Kogay, Yuri I. Wolf, Eugene V. Koonin","doi":"10.1111/1462-2920.16630","DOIUrl":"https://doi.org/10.1111/1462-2920.16630","url":null,"abstract":"Horizontal gene transfer (HGT) is a fundamental process in prokaryotic evolution, contributing significantly to diversification and adaptation. HGT is typically facilitated by mobile genetic elements (MGEs), such as conjugative plasmids and phages, which often impose fitness costs on their hosts. However, a considerable number of bacterial genes are involved in defence mechanisms that limit the propagation of MGEs, suggesting they may actively restrict HGT. In our study, we investigated whether defence systems limit HGT by examining the relationship between the HGT rate and the presence of 73 defence systems across 12 bacterial species. We discovered that only six defence systems, three of which were different CRISPR-Cas subtypes, were associated with a reduced gene gain rate at the species evolution scale. Hosts of these defence systems tend to have a smaller pangenome size and fewer phage-related genes compared to genomes without these systems. This suggests that these defence mechanisms inhibit HGT by limiting prophage integration. We hypothesize that the restriction of HGT by defence systems is species-specific and depends on various ecological and genetic factors, including the burden of MGEs and the fitness effect of HGT in bacterial populations.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"26 4","pages":""},"PeriodicalIF":5.1,"publicationDate":"2024-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.16630","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140632035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling the culturable and non-culturable actinobacterial diversity in two macroalgae species from the northern Portuguese coast 揭示葡萄牙北部海岸两种大型藻类中可培养和不可培养放线菌的多样性

IF 5.1 2区生物学

Environmental microbiology Pub Date : 2024-04-16 DOI: 10.1111/1462-2920.16620

Mariana Girão, Diogo A. M. Alexandrino, Weiwei Cao, Isabel Costa, Zhongjun Jia, Maria F. Carvalho

{"title":"Unveiling the culturable and non-culturable actinobacterial diversity in two macroalgae species from the northern Portuguese coast","authors":"Mariana Girão, Diogo A. M. Alexandrino, Weiwei Cao, Isabel Costa, Zhongjun Jia, Maria F. Carvalho","doi":"10.1111/1462-2920.16620","DOIUrl":"https://doi.org/10.1111/1462-2920.16620","url":null,"abstract":"Actinomycetota, associated with macroalgae, remains one of the least explored marine niches. The secondary metabolism of Actinomycetota, the primary microbial source of compounds relevant to biotechnology, continues to drive research into the distribution, dynamics, and metabolome of these microorganisms. In this study, we employed a combination of traditional cultivation and metagenomic analysis to investigate the diversity of Actinomycetota in two native macroalgae species from the Portuguese coast. We obtained and taxonomically identified a collection of 380 strains, which were distributed across 12 orders, 15 families, and 25 genera affiliated with the Actinomycetia class, with Streptomyces making up approximately 60% of the composition. Metagenomic results revealed the presence of Actinomycetota in both Chondrus crispus and Codium tomentosum datasets, with relative abundances of 11% and 2%, respectively. This approach identified 12 orders, 16 families, and 17 genera affiliated with Actinomycetota, with minimal overlap with the cultivation results. Acidimicrobiales emerged as the dominant actinobacterial order in both macroalgae, although no strain affiliated with this taxonomic group was successfully isolated. Our findings suggest that macroalgae represent a hotspot for Actinomycetota. The synergistic use of both culture-dependent and independent approaches proved beneficial, enabling the identification and recovery of not only abundant but also rare taxonomic members.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"26 4","pages":""},"PeriodicalIF":5.1,"publicationDate":"2024-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.16620","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140559534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clonal genomic population structure of Beauveria brongniartii and Beauveria pseudobassiana: Pathogens of the common European cockchafer (Melolontha melolontha L.) Beauveria brongniartii 和 Beauveria pseudobassiana 的克隆基因组种群结构：欧洲普通鸡毛虫（Melolontha melolontha L.）的病原体

IF 5.1 2区生物学

Environmental microbiology Pub Date : 2024-04-15 DOI: 10.1111/1462-2920.16612

Chiara Pedrazzini, Stephen A. Rehner, Hermann Strasser, Niklaus Zemp, Rolf Holderegger, Franco Widmer, Jürg Enkerli

{"title":"Clonal genomic population structure of Beauveria brongniartii and Beauveria pseudobassiana: Pathogens of the common European cockchafer (Melolontha melolontha L.)","authors":"Chiara Pedrazzini, Stephen A. Rehner, Hermann Strasser, Niklaus Zemp, Rolf Holderegger, Franco Widmer, Jürg Enkerli","doi":"10.1111/1462-2920.16612","DOIUrl":"https://doi.org/10.1111/1462-2920.16612","url":null,"abstract":"Beauveria brongniartii is a fungal pathogen that infects the beetle Melolontha melolontha, a significant agricultural pest in Europe. While research has primarily focused on the use of B. brongniartii for controlling M. melolontha, the genomic structure of the B. brongniartii population remains unknown. This includes whether its structure is influenced by its interaction with M. melolontha, the timing of beetle-swarming flights, geographical factors, or reproductive mode. To address this, we analysed genome-wide SNPs to infer the population genomics of Beauveria spp., which were isolated from infected M. melolontha adults in an Alpine region. Surprisingly, only one-third of the isolates were identified as B. brongniartii, while two-thirds were distributed among cryptic taxa within B. pseudobassiana, a fungal species not previously recognized as a pathogen of M. melolontha. Given the prevalence of B. pseudobassiana, we conducted analyses on both species. We found no spatial or temporal genomic patterns within either species and no correlation with the population structure of M. melolontha, suggesting that the dispersal of the fungi is independent of the beetle. Both species exhibited clonal population structures, with B. brongniartii fixed for one mating type and B. pseudobassiana displaying both mating types. This implies that factors other than mating compatibility limit sexual reproduction. We conclude that the population genomic structure of Beauveria spp. is primarily influenced by predominant asexual reproduction and dispersal.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"26 4","pages":""},"PeriodicalIF":5.1,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.16612","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140556226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microorganisms uniquely capture and predict stony coral tissue loss disease and hurricane disturbance impacts on US Virgin Island reefs 微生物独特捕捉和预测美属维尔京群岛珊瑚礁石珊瑚组织损失疾病和飓风干扰的影响

IF 5.1 2区生物学

Environmental microbiology Pub Date : 2024-04-04 DOI: 10.1111/1462-2920.16610

Cynthia C. Becker, Laura Weber, Joel K. Llopiz, T. Aran Mooney, Amy Apprill

{"title":"Microorganisms uniquely capture and predict stony coral tissue loss disease and hurricane disturbance impacts on US Virgin Island reefs","authors":"Cynthia C. Becker, Laura Weber, Joel K. Llopiz, T. Aran Mooney, Amy Apprill","doi":"10.1111/1462-2920.16610","DOIUrl":"https://doi.org/10.1111/1462-2920.16610","url":null,"abstract":"Coral reef ecosystems are now commonly affected by major climate and disease disturbances. Disturbance impacts are typically recorded using reef benthic cover, but this may be less reflective of other ecosystem processes. To explore the potential for reef water-based disturbance indicators, we conducted a 7-year time series on US Virgin Island reefs where we examined benthic cover and reef water nutrients and microorganisms from 2016 to 2022, which included two major disturbances: hurricanes Irma and Maria in 2017 and the stony coral tissue loss disease outbreak starting in 2020. The disease outbreak coincided with the largest changes in the benthic habitat, with increases in the percent cover of turf algae and Ramicrusta, an invasive alga. While sampling timepoint contributed most to changes in reef water nutrient composition and microbial community beta diversity, both disturbances led to increases in ammonium concentration, a mechanism likely contributing to observed microbial community shifts. We identified 10 microbial taxa that were sensitive and predictive of increasing ammonium concentration. This included the decline of the oligotrophic and photoautotrophic Prochlorococcus and the enrichment of heterotrophic taxa. As disturbances impact reefs, the changing nutrient and microbial regimes may foster a type of microbialization, a process that hastens reef degradation.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"26 4","pages":""},"PeriodicalIF":5.1,"publicationDate":"2024-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.16610","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140348564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preventing multi-resistance: New insights for managing fungal adaptation 预防多重抗药性：管理真菌适应性的新见解

IF 5.1 2区生物学

Environmental microbiology Pub Date : 2024-04-03 DOI: 10.1111/1462-2920.16614

Agathe Ballu, Claire Ugazio, Clémentine Duplaix, Alicia Noly, Juerg Wullschleger, Stefano F. F. Torriani, Anne Dérédec, Florence Carpentier, Anne-Sophie Walker

{"title":"Preventing multi-resistance: New insights for managing fungal adaptation","authors":"Agathe Ballu, Claire Ugazio, Clémentine Duplaix, Alicia Noly, Juerg Wullschleger, Stefano F. F. Torriani, Anne Dérédec, Florence Carpentier, Anne-Sophie Walker","doi":"10.1111/1462-2920.16614","DOIUrl":"https://doi.org/10.1111/1462-2920.16614","url":null,"abstract":"Sustainable crop protection is vital for food security, yet it is under threat due to the adaptation of a diverse and evolving pathogen population. Resistance can be managed by maximising the diversity of selection pressure through dose variation and the spatial and temporal combination of active ingredients. This study explores the interplay between operational drivers for maximising the sustainability of management strategies in relation to the resistance status of fungal populations. We applied an experimental evolution approach to three artificial populations of Zymoseptoria tritici, an economically significant wheat pathogen, each differing in initial resistance status. Our findings reveal that diversified selection pressure curtails the selection of resistance in naïve populations and those with low frequencies of single resistance. Increasing the number of modes of action most effectively delays resistance development, surpassing the increase in the number of fungicides, fungicide choice based on resistance risk, and temporal variation in fungicide exposure. However, this approach favours generalism in the evolved populations. The prior presence of multiple resistant isolates and their subsequent selection in populations override the effects of diversity in management strategies, thereby invalidating any universal ranking. Therefore, the initial resistance composition must be specifically considered in sustainable resistance management to address real-world field situations.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"26 4","pages":""},"PeriodicalIF":5.1,"publicationDate":"2024-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.16614","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140345746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Experimental evidence of d-glutamate racemase activity in the uncultivated bacterium Candidatus Saccharimonas aalborgensis 实验证明未培养的奥尔堡酵母菌具有 d-谷氨酸消旋酶活性

IF 5.1 2区生物学

Environmental microbiology Pub Date : 2024-04-01 DOI: 10.1111/1462-2920.16621

Marcos Peñalver, Alberto Paradela, César Palacios-Cuéllar, M. Graciela Pucciarelli, Francisco García-del Portillo

{"title":"Experimental evidence of d-glutamate racemase activity in the uncultivated bacterium Candidatus Saccharimonas aalborgensis","authors":"Marcos Peñalver, Alberto Paradela, César Palacios-Cuéllar, M. Graciela Pucciarelli, Francisco García-del Portillo","doi":"10.1111/1462-2920.16621","DOIUrl":"https://doi.org/10.1111/1462-2920.16621","url":null,"abstract":"The Candidate Phyla Radiation (CPR) encompasses widespread uncultivated bacteria with reduced genomes and limited metabolic capacities. Most CPR bacteria lack the minimal set of enzymes required for peptidoglycan (PG) synthesis, leaving it unclear how these bacteria produce this essential envelope component. In this study, we analysed the distribution of d-amino acid racemases that produce the universal PG components d-glutamate (d-Glu) or d-alanine (d-Ala). We also examined moonlighting enzymes that synthesize d-Glu or d-Ala. Unlike other phyla in the domain Bacteria, CPR bacteria do not exhibit these moonlighting activities and have, at most, one gene encoding either a Glu or Ala racemase. One of these ‘orphan’ racemases is a predicted Glu racemase (MurICPR) from the CPR bacterium Candidatus Saccharimonas aalborgenesis. The expression of MurICPR restores the growth of a Salmonella d-Glu auxotroph lacking its endogenous racemase and results in the substitution of l-Ala by serine as the first residue in a fraction of the PG stem peptides. In vitro, MurICPR exclusively racemizes Glu as a substrate. Therefore, Ca. Saccharimonas aalborgensis may couple Glu racemization to serine and d-Glu incorporation into the stem peptide. Our findings provide the first insights into the synthesis of PG by an uncultivated environmental bacterium and illustrate how to experimentally test enzymatic activities from CPR bacteria related to PG metabolism.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"26 4","pages":""},"PeriodicalIF":5.1,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.16621","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140333285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The distinctive weathering crust habitat of a High Arctic glacier comprises discrete microbial micro-habitats 北极高纬度冰川独特的风化壳生境由离散的微生物微生境组成

IF 5.1 2区生物学

Environmental microbiology Pub Date : 2024-04-01 DOI: 10.1111/1462-2920.16617

Sara M. E. Rassner, Joseph M. Cook, Andrew C. Mitchell, Ian T. Stevens, Tristram D. L. Irvine-Fynn, Andrew J. Hodson, Arwyn Edwards

{"title":"The distinctive weathering crust habitat of a High Arctic glacier comprises discrete microbial micro-habitats","authors":"Sara M. E. Rassner, Joseph M. Cook, Andrew C. Mitchell, Ian T. Stevens, Tristram D. L. Irvine-Fynn, Andrew J. Hodson, Arwyn Edwards","doi":"10.1111/1462-2920.16617","DOIUrl":"https://doi.org/10.1111/1462-2920.16617","url":null,"abstract":"Sunlight penetrates the ice surfaces of glaciers and ice sheets, forming a water-bearing porous ice matrix known as the weathering crust. This crust is home to a significant microbial community. Despite the potential implications of microbial processes in the weathering crust for glacial melting, biogeochemical cycles, and downstream ecosystems, there have been few explorations of its microbial communities. In our study, we used 16S rRNA gene sequencing and shotgun metagenomics of a Svalbard glacier surface catchment to characterise the microbial communities within the weathering crust, their origins and destinies, and the functional potential of the weathering crust metagenome. Our findings reveal that the bacterial community in the weathering crust is distinct from those in upstream and downstream habitats. However, it comprises two separate micro-habitats, each with different taxa and functional categories. The interstitial porewater is dominated by Polaromonas, influenced by the transfer of snowmelt, and exported via meltwater channels. In contrast, the ice matrix is dominated by Hymenobacter, and its metagenome exhibits a diverse range of functional adaptations. Given that the global weathering crust area and the subsequent release of microbes from it are strongly responsive to climate projections for the rest of the century, our results underscore the pressing need to integrate the microbiome of the weathering crust with other communities and processes in glacial ecosystems.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"26 4","pages":""},"PeriodicalIF":5.1,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.16617","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140333287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Wolbachia infection negatively impacts Drosophila simulans heat tolerance in a strain- and trait-specific manner 沃尔巴克氏体感染以品系和性状特异性的方式对果蝇的耐热性产生负面影响

IF 5.1 2区生物学

Environmental microbiology Pub Date : 2024-04-01 DOI: 10.1111/1462-2920.16609

Liam F. Ferguson, Perran A. Ross, Belinda van Heerwaarden

{"title":"Wolbachia infection negatively impacts Drosophila simulans heat tolerance in a strain- and trait-specific manner","authors":"Liam F. Ferguson, Perran A. Ross, Belinda van Heerwaarden","doi":"10.1111/1462-2920.16609","DOIUrl":"https://doi.org/10.1111/1462-2920.16609","url":null,"abstract":"The susceptibility of insects to rising temperatures has largely been measured by their ability to survive thermal extremes. However, the capacity for maternally inherited endosymbionts to influence insect heat tolerance has been overlooked. Further, while some studies have addressed the impact of heat on traits like fertility, which can decline at temperatures below lethal thermal limits, none have considered the impact of endosymbionts. Here, we assess the impact of three Wolbachia strains (wRi, wAu and wNo) on the survival and fertility of Drosophila simulans exposed to heat stress during development or as adults. The effect of Wolbachia infection on heat tolerance was generally small and trait/strain specific. Only the wNo infection significantly reduced the survival of adult males after a heat shock. When exposed to fluctuating heat stress during development, the wRi and wAu strains reduced egg-to-adult survival but only the wNo infection reduced male fertility. Wolbachia densities of all three strains decreased under developmental heat stress, but reductions occurred at temperatures above those that reduced host fertility. These findings emphasize the necessity to account for endosymbionts and their effect on both survival and fertility when investigating insect responses to heat stress.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"26 4","pages":""},"PeriodicalIF":5.1,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.16609","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140333288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial hitchhikers on microplastics: The exchange of aquatic microbes across distinct aquatic habitats 微塑料上的微生物搭便车：水生微生物在不同水生栖息地之间的交换。

IF 5.1 2区生物学

Environmental microbiology Pub Date : 2024-04-01 DOI: 10.1111/1462-2920.16618

Máté Vass, Kesava Priyan Ramasamy, Agneta Andersson

{"title":"Microbial hitchhikers on microplastics: The exchange of aquatic microbes across distinct aquatic habitats","authors":"Máté Vass, Kesava Priyan Ramasamy, Agneta Andersson","doi":"10.1111/1462-2920.16618","DOIUrl":"10.1111/1462-2920.16618","url":null,"abstract":"Microplastics (MPs) have the potential to modify aquatic microbial communities and distribute microorganisms, including pathogens. This poses a potential risk to aquatic life and human health. Despite this, the fate of ‘hitchhiking’ microbes on MPs that traverse different aquatic habitats remains largely unknown. To address this, we conducted a 50-day microcosm experiment, manipulating estuarine conditions to study the exchange of bacteria and microeukaryotes between river, sea and plastisphere using a long-read metabarcoding approach. Our findings revealed a significant increase in bacteria on the plastisphere, including Pseudomonas, Sphingomonas, Hyphomonas, Brevundimonas, Aquabacterium and Thalassolituus, all of which are known for their pollutant degradation capabilities, specifically polycyclic aromatic hydrocarbons. We also observed a strong association of plastic-degrading fungi (i.e., Cladosporium and Plectosphaerella) and early-diverging fungi (Cryptomycota, also known as Rozellomycota) with the plastisphere. Sea MPs were primarily colonised by fungi (70%), with a small proportion of river-transported microbes (1%–4%). The mere presence of MPs in seawater increased the relative abundance of planktonic fungi from 2% to 25%, suggesting significant exchanges between planktonic and plastisphere communities. Using microbial source tracking, we discovered that MPs only dispersed 3.5% and 5.5% of river bacterial and microeukaryotic communities into the sea, respectively. Hence, although MPs select and facilitate the dispersal of ecologically significant microorganisms, drastic compositional changes across distinct aquatic habitats are unlikely.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"26 4","pages":""},"PeriodicalIF":5.1,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.16618","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140335188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multiple toxins and a protease contribute to the aphid-killing ability of Pseudomonas fluorescens PpR24 多种毒素和一种蛋白酶增强了荧光假单胞菌 PpR24 杀死蚜虫的能力。

IF 5.1 2区生物学

Environmental microbiology Pub Date : 2024-04-01 DOI: 10.1111/1462-2920.16604

Deepa Paliwal, Mojgan Rabiey, Tim H. Mauchline, Keywan Hassani-Pak, Ralf Nauen, Carol Wagstaff, Simon Andrews, Chris Bass, Robert W. Jackson

{"title":"Multiple toxins and a protease contribute to the aphid-killing ability of Pseudomonas fluorescens PpR24","authors":"Deepa Paliwal, Mojgan Rabiey, Tim H. Mauchline, Keywan Hassani-Pak, Ralf Nauen, Carol Wagstaff, Simon Andrews, Chris Bass, Robert W. Jackson","doi":"10.1111/1462-2920.16604","DOIUrl":"10.1111/1462-2920.16604","url":null,"abstract":"Aphids are globally important pests causing damage to a broad range of crops. Due to insecticide resistance, there is an urgent need to develop alternative control strategies. In our previous work, we found Pseudomonas fluorescens PpR24 can orally infect and kill the insecticide-resistant green-peach aphid (Myzus persicae). However, the genetic basis of the insecticidal capability of PpR24 remains unclear. Genome sequencing of PpR24 confirmed the presence of various insecticidal toxins such as Tc (toxin complexes), Rhs (rearrangement hotspot) elements, and other insect-killing proteases. Upon aphids infection with PpR24, RNA-Seq analysis revealed 193 aphid genes were differentially expressed with down-regulation of 16 detoxification genes. In addition, 1325 PpR24 genes (542 were upregulated and 783 downregulated) were subject to differential expression, including genes responsible for secondary metabolite biosynthesis, the iron-restriction response, oxidative stress resistance, and virulence factors. Single and double deletion of candidate virulence genes encoding a secreted protease (AprX) and four toxin components (two TcA-like; one TcB-like; one TcC-like insecticidal toxins) showed that all five genes contribute significantly to aphid killing, particularly AprX. This comprehensive host–pathogen transcriptomic analysis provides novel insight into the molecular basis of bacteria-mediated aphid mortality and the potential of PpR24 as an effective biocontrol agent.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"26 4","pages":""},"PeriodicalIF":5.1,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.16604","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140335189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0