Environmental microbiology最新文献_第10页

How good are global DNA-based environmental surveys for detecting all protist diversity? Arcellinida as an example of biased representation 基于 DNA 的全球环境调查在检测所有原生生物多样性方面的效果如何？以 Arcellinida 为例，说明其代表性存在偏差。

IF 5.1 2区生物学

Environmental microbiology Pub Date : 2024-03-20 DOI: 10.1111/1462-2920.16606

Fernando Useros, Iván García-Cunchillos, Nicolas Henry, Cédric Berney, Enrique Lara

{"title":"How good are global DNA-based environmental surveys for detecting all protist diversity? Arcellinida as an example of biased representation","authors":"Fernando Useros, Iván García-Cunchillos, Nicolas Henry, Cédric Berney, Enrique Lara","doi":"10.1111/1462-2920.16606","DOIUrl":"10.1111/1462-2920.16606","url":null,"abstract":"Metabarcoding approaches targeting microeukaryotes have deeply changed our vision of protist environmental diversity. The public repository EukBank consists of 18S v4 metabarcodes from 12,672 samples worldwide. To estimate how far this database provides a reasonable overview of all eukaryotic diversity, we used Arcellinida (lobose testate amoebae) as a case study. We hypothesised that (1) this approach would allow the discovery of unexpected diversity, but also that (2) some groups would be underrepresented because of primer/sequencing biases. Most of the Arcellinida sequences appeared in freshwater and soil, but their abundance and diversity appeared underrepresented. Moreover, 84% of ASVs belonged to the suborder Phryganellina, a supposedly species-poor clade, whereas the best-documented suborder (Glutinoconcha, 600 described species) was only marginally represented. We explored some possible causes of these biases. Mismatches in the primer-binding site seem to play a minor role. Excessive length of the target region could explain some of these biases, but not all. There must be some other unknown factors involved. Altogether, while metabarcoding based on ribosomal genes remains a good first approach to document microbial eukaryotic clades, alternative approaches based on other genes or sequencing techniques must be considered for an unbiased picture of the diversity of some groups.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":null,"pages":null},"PeriodicalIF":5.1,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.16606","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140179473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Employing a triple metabarcoding approach to differentiate active, dormant and dead microeukaryotes in sediments 采用三重代谢编码方法区分沉积物中活跃、休眠和死亡的微真核细胞。

IF 5.1 2区生物学

Environmental microbiology Pub Date : 2024-03-19 DOI: 10.1111/1462-2920.16615

Huiwen Deng, Cui He, Alexandra Z. Worden, Jun Gong

{"title":"Employing a triple metabarcoding approach to differentiate active, dormant and dead microeukaryotes in sediments","authors":"Huiwen Deng, Cui He, Alexandra Z. Worden, Jun Gong","doi":"10.1111/1462-2920.16615","DOIUrl":"10.1111/1462-2920.16615","url":null,"abstract":"Microbial communities are commonly characterised through the metabarcoding of environmental DNA. This DNA originates from both viable (including dormant and active) and dead organisms, leading to recent efforts to distinguish between these states. In this study, we further these approaches by distinguishing not only between viable and dead cells but also between dormant and actively growing cells. This is achieved by sequencing both rRNA and rDNA, in conjunction with propidium monoazide cross-linked rDNA, to partition the active, dormant and relic fractions in environmental samples. We apply this method to characterise the diversity and assemblage structure of these fractions of microeukaryotes in intertidal sediments during a wet-dry-rewet incubation cycle. Our findings indicate that a significant proportion of microeukaryotic phylotypes detected in the total rDNA pools originate from dormant and relic microeukaryotes in the sediments, both in terms of richness (dormant, 13 ± 2%; relic, 47 ± 5%) and read abundance (dormant, 20 ± 7%; relic, 14 ± 5%). The richness and sequence proportion of dormant microeukaryotes notably increase during the transition from wet to dry conditions. Statistical analyses suggest that the dynamics of diversity and assemblage structure across different activity fractions are influenced by various environmental drivers. Our strategy offers a versatile approach that can be adapted to characterise other microbes in a wide range of environments.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":null,"pages":null},"PeriodicalIF":5.1,"publicationDate":"2024-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140157852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phototrophic Fe(II) oxidation by Rhodopseudomonas palustris TIE-1 in organic and Fe(II)-rich conditions 在富含有机物和铁(II)的条件下，古朴红单胞菌 TIE-1 的光营养铁(II)氧化作用

IF 5.1 2区生物学

Environmental microbiology Pub Date : 2024-03-19 DOI: 10.1111/1462-2920.16608

Verena Nikeleit, Markus Maisch, James M. Byrne, Caroline Harwood, Andreas Kappler, Casey Bryce

{"title":"Phototrophic Fe(II) oxidation by Rhodopseudomonas palustris TIE-1 in organic and Fe(II)-rich conditions","authors":"Verena Nikeleit, Markus Maisch, James M. Byrne, Caroline Harwood, Andreas Kappler, Casey Bryce","doi":"10.1111/1462-2920.16608","DOIUrl":"https://doi.org/10.1111/1462-2920.16608","url":null,"abstract":"Rhodopseudomonas palustris TIE-1 grows photoautotrophically with Fe(II) as an electron donor and photoheterotrophically with a variety of organic substrates. However, it is unclear whether R. palustris TIE-1 conducts Fe(II) oxidation in conditions where organic substrates and Fe(II) are available simultaneously. In addition, the effect of organic co-substrates on Fe(II) oxidation rates or the identity of Fe(III) minerals formed is unknown. We incubated R. palustris TIE-1 with 2 mM Fe(II), amended with 0.6 mM organic co-substrate, and in the presence/absence of CO2. We found that in the absence of CO2, only the organic co-substrates acetate, lactate and pyruvate, but not Fe(II), were consumed. When CO2 was present, Fe(II) and all organic substrates were consumed. Acetate, butyrate and pyruvate were consumed before Fe(II) oxidation commenced, whereas lactate and glucose were consumed at the same time as Fe(II) oxidation proceeded. Lactate, pyruvate and glucose increased the Fe(II) oxidation rate significantly (by up to threefold in the case of lactate). 57Fe Mössbauer spectroscopy revealed that short-range ordered Fe(III) oxyhydroxides were formed under all conditions. This study demonstrates phototrophic Fe(II) oxidation proceeds even in the presence of organic compounds, and that the simultaneous oxidation of organic substrates can stimulate Fe(II) oxidation.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":null,"pages":null},"PeriodicalIF":5.1,"publicationDate":"2024-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.16608","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140164355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nontarget impacts of neonicotinoids on nectar-inhabiting microbes 新烟碱对花蜜栖息微生物的非目标影响。

IF 5.1 2区生物学

Environmental microbiology Pub Date : 2024-03-17 DOI: 10.1111/1462-2920.16603

Jacob M. Cecala, Rachel L. Vannette

{"title":"Nontarget impacts of neonicotinoids on nectar-inhabiting microbes","authors":"Jacob M. Cecala, Rachel L. Vannette","doi":"10.1111/1462-2920.16603","DOIUrl":"10.1111/1462-2920.16603","url":null,"abstract":"Plant-systemic neonicotinoid (NN) insecticides can exert non-target impacts on organisms like beneficial insects and soil microbes. NNs can affect plant microbiomes, but we know little about their effects on microbial communities that mediate plant-insect interactions, including nectar-inhabiting microbes (NIMs). Here we employed two approaches to assess the impacts of NN exposure on several NIM taxa. First, we assayed the in vitro effects of six NN compounds on NIM growth using plate assays. Second, we inoculated a standardised NIM community into the nectar of NN-treated canola (Brassica napus) and assessed microbial survival and growth after 24 h. With few exceptions, in vitro NN exposure tended to decrease bacterial growth metrics. However, the magnitude of the decrease and the NN concentrations at which effects were observed varied substantially across bacteria. Yeasts showed no consistent in vitro response to NNs. In nectar, we saw no effects of NN treatment on NIM community metrics. Rather, NIM abundance and diversity responded to inherent plant qualities like nectar volume. In conclusion, we found no evidence that NIMs respond to field-relevant NN levels in nectar within 24 h, but our study suggests that context, specifically assay methods, time and plant traits, is important in assaying the effects of NNs on microbial communities.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":null,"pages":null},"PeriodicalIF":5.1,"publicationDate":"2024-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.16603","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140143042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential structure and function of phosphorus-mineralizing microbial communities in organic and upper mineral soil horizons across a temperate rainforest chronosequence 温带雨林时序中有机土壤层和上层矿质土壤层中磷矿化微生物群落的结构和功能差异。

IF 5.1 2区生物学

Environmental microbiology Pub Date : 2024-03-14 DOI: 10.1111/1462-2920.16600

Kari E. Dunfield, Eduardo K. Mitter, Alan E. Richardson, Jonathan R. Gaiero, Kamini Khosla, Xiaodong Chen, Andrew Wells, Philip M. Haygarth, Leo M. Condron

{"title":"Differential structure and function of phosphorus-mineralizing microbial communities in organic and upper mineral soil horizons across a temperate rainforest chronosequence","authors":"Kari E. Dunfield, Eduardo K. Mitter, Alan E. Richardson, Jonathan R. Gaiero, Kamini Khosla, Xiaodong Chen, Andrew Wells, Philip M. Haygarth, Leo M. Condron","doi":"10.1111/1462-2920.16600","DOIUrl":"10.1111/1462-2920.16600","url":null,"abstract":"Microbial community structure and function were assessed in the organic and upper mineral soil across a ~4000-year dune-based chronosequence at Big Bay, New Zealand, where total P declined and the proportional contribution of organic soil in the profile increased with time. We hypothesized that the organic and mineral soils would show divergent community evolution over time with a greater dependency on the functionality of phosphatase genes in the organic soil layer as it developed. The structure of bacterial, fungal, and phosphatase-harbouring communities was examined in both horizons across 3 dunes using amplicon sequencing, network analysis, and qPCR. The soils showed a decline in pH and total phosphorus (P) over time with an increase in phosphatase activity. The organic horizon had a wider diversity of Class A (phoN/phoC) and phoD-harbouring communities and a more complex microbiome, with hub taxa that correlated with P. Bacterial diversity declined in both horizons over time, with enrichment of Planctomycetes and Acidobacteria. More complex fungal communities were evident in the youngest dune, transitioning to a dominance of Ascomycota in both soil horizons. Higher phosphatase activity in older dunes was driven by less diverse P-mineralizing communities, especially in the organic horizon.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":null,"pages":null},"PeriodicalIF":5.1,"publicationDate":"2024-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.16600","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140119171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacterial rarity in a subarctic stream network: Biodiversity patterns, assembly mechanisms and types of rarity 亚北极溪流网络中的细菌稀有性：生物多样性模式、组装机制和稀有类型。

IF 5.1 2区生物学

Environmental microbiology Pub Date : 2024-03-14 DOI: 10.1111/1462-2920.16592

Jacqueline Malazarte, Timo Muotka, Jussi Jyväsjärvi, Kaisa Lehosmaa, Kaisa-Riikka Mustonen, Laura Tarvainen, Kaisa-Leena Huttunen

{"title":"Bacterial rarity in a subarctic stream network: Biodiversity patterns, assembly mechanisms and types of rarity","authors":"Jacqueline Malazarte, Timo Muotka, Jussi Jyväsjärvi, Kaisa Lehosmaa, Kaisa-Riikka Mustonen, Laura Tarvainen, Kaisa-Leena Huttunen","doi":"10.1111/1462-2920.16592","DOIUrl":"10.1111/1462-2920.16592","url":null,"abstract":"Dendritic stream networks are an intriguing subject for exploring the spatial and temporal variability of the rare and common bacterial biosphere, yet very few such studies have been conducted. We sampled riverine bacterioplankton at 13 sites in a subarctic riverine network across 3 years, with five sampling times each year. Ordinations showed a consistent pattern of downstream shift for both rare and abundant subcommunities. We also detected a temporal signal, with seasonal community shifts reflecting changes in water temperature and groundwater contribution, and an inter-annual pattern where the year 2018 differed from other years. Phylogenetic turnover of the rare subcommunity indicated homogeneous selection, whereas the abundant subcommunity was mainly stochastically structured. Transiently rare taxa were the dominant type of rarity with the highest proportion at the headwater regions. The bacterioplankton community was characterized by a small group of core taxa that occurred at most sites with little temporal variation, a very large number of permanently or transiently rare taxa, and taxa shifting through time between the rare and abundant biosphere. While this basic structure could have been detected with less extensive temporal replication, a comprehensive understanding of the rare biosphere in riverine bacterioplankton can only be achieved via inter-annual, spatially replicated sampling that covers the whole stream network.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":null,"pages":null},"PeriodicalIF":5.1,"publicationDate":"2024-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.16592","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140119170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Environmental filtering governs consistent vertical zonation in sedimentary microbial communities across disconnected mountain lakes 环境过滤作用于互不相连的高山湖泊中沉积微生物群落的一致垂直分带。

IF 5.1 2区生物学

Environmental microbiology Pub Date : 2024-03-13 DOI: 10.1111/1462-2920.16607

Jordan M. Von Eggers, Nathan I. Wisnoski, John W. Calder, Eric Capo, Dulcinea V. Groff, Amy C. Krist, Bryan Shuman

{"title":"Environmental filtering governs consistent vertical zonation in sedimentary microbial communities across disconnected mountain lakes","authors":"Jordan M. Von Eggers, Nathan I. Wisnoski, John W. Calder, Eric Capo, Dulcinea V. Groff, Amy C. Krist, Bryan Shuman","doi":"10.1111/1462-2920.16607","DOIUrl":"10.1111/1462-2920.16607","url":null,"abstract":"Subsurface microorganisms make up the majority of Earth's microbial biomass, but ecological processes governing surface communities may not explain community patterns at depth because of burial. Depth constrains dispersal and energy availability, and when combined with geographic isolation across landscapes, may influence community assembly. We sequenced the 16S rRNA gene of bacteria and archaea from 48 sediment cores across 36 lakes in four disconnected mountain ranges in Wyoming, USA and used null models to infer assembly processes across depth, spatial isolation, and varying environments. Although we expected strong dispersal limitations across these isolated settings, community composition was primarily shaped by environmental selection. Communities consistently shifted from domination by organisms that degrade organic matter at the surface to methanogenic, low-energy adapted taxa in deeper zones. Stochastic processes—like dispersal limitation—contributed to differences among lakes, but because these effects weakened with depth, selection processes ultimately governed subsurface microbial biogeography.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":null,"pages":null},"PeriodicalIF":5.1,"publicationDate":"2024-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140109715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aphid populations are frequently infected with facultative endosymbionts 蚜虫种群经常感染兼性内生菌。

IF 5.1 2区生物学

Environmental microbiology Pub Date : 2024-03-08 DOI: 10.1111/1462-2920.16599

S. Helena Donner, Marijn Slingerland, Mariska M. Beekman, Arthur Comte, Marcel Dicke, Bas J. Zwaan, Bart A. Pannebakker, Eveline C. Verhulst

{"title":"Aphid populations are frequently infected with facultative endosymbionts","authors":"S. Helena Donner, Marijn Slingerland, Mariska M. Beekman, Arthur Comte, Marcel Dicke, Bas J. Zwaan, Bart A. Pannebakker, Eveline C. Verhulst","doi":"10.1111/1462-2920.16599","DOIUrl":"10.1111/1462-2920.16599","url":null,"abstract":"The occurrence of facultative endosymbionts has been studied in many commercially important crop pest aphids, but their occurrence and effects in non-commercial aphid species in natural populations have received less attention. We screened 437 aphid samples belonging to 106 aphid species for the eight most common facultative aphid endosymbionts. We found one or more facultative endosymbionts in 53% (56 of 106) of the species investigated. This likely underestimates the situation in the field because facultative endosymbionts are often present in only some colonies of an aphid species. Oligophagous aphid species carried facultative endosymbionts significantly more often than monophagous species. We did not find a significant correlation between ant tending and facultative endosymbiont presence. In conclusion, we found that facultative endosymbionts are common among aphid populations. This study is, to our knowledge, the first of its kind in the Netherlands and provides a basis for future research in this field. For instance, it is still unknown in what way many of these endosymbionts affect their hosts, which is important for determining the importance of facultative endosymbionts to community dynamics.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":null,"pages":null},"PeriodicalIF":5.1,"publicationDate":"2024-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.16599","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140065012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The ectomycorrhizal fungus Paxillus ammoniavirescens influences the effects of salinity on loblolly pine in response to potassium availability 外生菌根真菌 Paxillus ammoniavirescens 可根据钾的供应情况影响盐度对龙柏松的影响。

IF 5.1 2区生物学

Environmental microbiology Pub Date : 2024-03-07 DOI: 10.1111/1462-2920.16597

Benjamin D. Rose, Marissa A. Dellinger, Clancy P. Larmour, Mira I. Polishook, Maria I. Higuita-Aguirre, Summi Dutta, Rachel L. Cook, Sabine D. Zimmermann, Kevin Garcia

{"title":"The ectomycorrhizal fungus Paxillus ammoniavirescens influences the effects of salinity on loblolly pine in response to potassium availability","authors":"Benjamin D. Rose, Marissa A. Dellinger, Clancy P. Larmour, Mira I. Polishook, Maria I. Higuita-Aguirre, Summi Dutta, Rachel L. Cook, Sabine D. Zimmermann, Kevin Garcia","doi":"10.1111/1462-2920.16597","DOIUrl":"10.1111/1462-2920.16597","url":null,"abstract":"Salinity is an increasing problem in coastal areas affected by saltwater intrusion, with deleterious effects on tree health and forest growth. Ectomycorrhizal (ECM) fungi may improve the salinity tolerance of host trees, but the impact of external potassium (K+) availability on these effects is still unclear. Here, we performed several experiments with the ECM fungus Paxillus ammoniavirescens and loblolly pine (Pinus taeda L.) in axenic and symbiotic conditions at limited or sufficient K+ and increasing sodium (Na+) concentrations. Growth rate, biomass, nutrient content, and K+ transporter expression levels were recorded for the fungus, and the colonization rate, root development parameters, biomass, and shoot nutrient accumulation were determined for mycorrhizal and non-mycorrhizal plants. P. ammoniavirescens was tolerant to high salinity, although growth and nutrient concentrations varied with K+ availability and increasing Na+ exposure. While loblolly pine root growth and development decreased with increasing salinity, ECM colonization was unaffected by pine response to salinity. The mycorrhizal influence on loblolly pine salinity response was strongly dependent on external K+ availability. This study reveals that P. ammoniavirescens can reduce Na+ accumulation of salt-exposed loblolly pine, but this effect depends on external K+ availability.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":null,"pages":null},"PeriodicalIF":5.1,"publicationDate":"2024-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.16597","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140049026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Active coexistence of the novel gammaproteobacterial methanotroph ‘Ca. Methylocalor cossyra’ CH1 and verrucomicrobial methanotrophs in acidic, hot geothermal soil 酸性高温地热土壤中新型伽马蛋白菌甲烷营养体'Ca.Methylocalor cossyra' CH1 和蛭石甲烷营养体在酸性高温地热土壤中的活跃共存。

IF 5.1 2区生物学

Environmental microbiology Pub Date : 2024-03-07 DOI: 10.1111/1462-2920.16602

Changqing Liu, Rob A. Schmitz, Arjan Pol, Carmen Hogendoorn, Daniël Verhagen, Stijn H. Peeters, Theo A. van Alen, Geert Cremers, Rob A. Mesman, Huub J. M. Op den Camp

{"title":"Active coexistence of the novel gammaproteobacterial methanotroph ‘Ca. Methylocalor cossyra’ CH1 and verrucomicrobial methanotrophs in acidic, hot geothermal soil","authors":"Changqing Liu, Rob A. Schmitz, Arjan Pol, Carmen Hogendoorn, Daniël Verhagen, Stijn H. Peeters, Theo A. van Alen, Geert Cremers, Rob A. Mesman, Huub J. M. Op den Camp","doi":"10.1111/1462-2920.16602","DOIUrl":"10.1111/1462-2920.16602","url":null,"abstract":"Terrestrial geothermal ecosystems are hostile habitats, characterized by large emissions of environmentally relevant gases such as CO2, CH4, H2S and H2. These conditions provide a niche for chemolithoautotrophic microorganisms. Methanotrophs of the phylum Verrucomicrobia, which inhabit these ecosystems, can utilize these gases and grow at pH levels below 1 and temperatures up to 65°C. In contrast, methanotrophs of the phylum Proteobacteria are primarily found in various moderate environments. Previously, novel verrucomicrobial methanotrophs were detected and isolated from the geothermal soil of the Favara Grande on the island of Pantelleria, Italy. The detection of pmoA genes, specific for verrucomicrobial and proteobacterial methanotrophs in this environment, and the partially overlapping pH and temperature growth ranges of these isolates suggest that these distinct phylogenetic groups could coexist in the environment. In this report, we present the isolation and characterization of a thermophilic and acid-tolerant gammaproteobacterial methanotroph (family Methylococcaceae) from the Favara Grande. This isolate grows at pH values ranging from 3.5 to 7.0 and temperatures from 35°C to 55°C, and diazotrophic growth was demonstrated. Its genome contains genes encoding particulate and soluble methane monooxygenases, XoxF- and MxaFI-type methanol dehydrogenases, and all enzymes of the Calvin cycle. For this novel genus and species, we propose the name ‘Candidatus Methylocalor cossyra’ CH1.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":null,"pages":null},"PeriodicalIF":5.1,"publicationDate":"2024-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.16602","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140058879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0