Environmental microbiology最新文献_第10页

Calcium and the ecology of photosynthesis in purple sulfur bacteria 钙与紫硫细菌光合作用生态学

IF 5.1 2区生物学

Environmental microbiology Pub Date : 2024-02-22 DOI: 10.1111/1462-2920.16591

Michael T. Madigan, W. Matthew Sattley, Yukihiro Kimura, Zheng-Yu Wang-Otomo

{"title":"Calcium and the ecology of photosynthesis in purple sulfur bacteria","authors":"Michael T. Madigan, W. Matthew Sattley, Yukihiro Kimura, Zheng-Yu Wang-Otomo","doi":"10.1111/1462-2920.16591","DOIUrl":"10.1111/1462-2920.16591","url":null,"abstract":"The ecological success of purple sulfur bacteria (PSB) is linked to their ability to collect near-infrared solar energy by membrane-integrated, pigment–protein photocomplexes. These include a Core complex containing both light-harvesting 1 (LH1) and reaction centre (RC) components (called the LH1–RC photocomplex) present in all PSB and a peripheral light-harvesting complex present in most but not all PSB. In research to explain the unusual absorption properties of the thermophilic purple sulfur bacterium Thermochromatium tepidum, Ca2+ was discovered bound to LH1 polypeptides in its LH1–RC; further work showed that calcium controls both the thermostability and unusual spectrum of the Core complex. Since then, Ca2+ has been found in the LH1–RC photocomplexes of several other PSB, including mesophilic species, but not in the LH1–RC of purple non-sulfur bacteria. Here we focus on four species of PSB—two thermophilic and two mesophilic—and describe how Ca2+ is integrated into and affects their photosynthetic machinery and why this previously overlooked divalent metal is a key nutrient for their ecological success.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":null,"pages":null},"PeriodicalIF":5.1,"publicationDate":"2024-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.16591","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139928771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

How to identify and quantify the members of the Bacillus genus? 如何识别和量化芽孢杆菌属的成员？

IF 5.1 2区生物学

Environmental microbiology Pub Date : 2024-02-21 DOI: 10.1111/1462-2920.16593

Xinming Xu, Ákos T. Kovács

引用次数: 0

Adding experimental precision to the realism of field observations: Plant communities structure bacterial communities in a glacier forefield 为实地观测的真实性增加实验精度：冰川前场的植物群落结构细菌群落。

IF 5.1 2区生物学

Environmental microbiology Pub Date : 2024-02-14 DOI: 10.1111/1462-2920.16590

Xie He, Maximilian Hanusch, Laura Böll, Alexander Lach, Tobias Seifert, Robert R. Junker

{"title":"Adding experimental precision to the realism of field observations: Plant communities structure bacterial communities in a glacier forefield","authors":"Xie He, Maximilian Hanusch, Laura Böll, Alexander Lach, Tobias Seifert, Robert R. Junker","doi":"10.1111/1462-2920.16590","DOIUrl":"10.1111/1462-2920.16590","url":null,"abstract":"Ecological studies are aligned along a realism–precision continuum ranging from field observations to controlled lab experiments that each have their own strengths and limitations. Ecological insight may be most robust when combining approaches. In field observations along a successional gradient, we found correlations between plant species composition and soil bacterial communities, while bacterial Shannon diversity was unrelated to vegetation characteristics. To add a causal understanding of the processes of bacterial community assembly, we designed lab experiments to specifically test the influence of plant composition on bacterial communities. Using soil and seeds from our field site, we added different combinations of surface-sterilised seeds to homogenised soil samples in microcosms and analysed bacterial communities 4 months later. Our results confirmed the field observations suggesting that experimental plant community composition shaped bacterial community composition, while Shannon diversity was unaffected. These results reflect intimate plant–bacteria interactions that are important drivers of plant health and community assembly. While this study provided insights into the role of plants underlying the assembly of bacterial communities, we did not experimentally manipulate other drivers of community assembly such as abiotic factors. Therefore, we recommend multi-factorial laboratory experiments to quantify the relative importance of different factors contributing to microbial composition.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":null,"pages":null},"PeriodicalIF":5.1,"publicationDate":"2024-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.16590","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139734770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Taxonomic and metabolic diversity of Actinomycetota isolated from faeces of a 28,000-year-old mammoth 从一头 2.8 万年前的猛犸象粪便中分离出的放线菌群的分类和代谢多样性。

IF 5.1 2区生物学

Environmental microbiology Pub Date : 2024-02-14 DOI: 10.1111/1462-2920.16589

Doris A. van Bergeijk, Hannah E. Augustijn, Somayah S. Elsayed, Joost Willemse, Victor J. Carrión, Chao Du, Mia Urem, Lena V. Grigoreva, Maksim Y. Cheprasov, Semyon Grigoriev, Hans Jansen, Bas Wintermans, Andries E. Budding, Herman P. Spaink, Marnix H. Medema, Gilles P. van Wezel

{"title":"Taxonomic and metabolic diversity of Actinomycetota isolated from faeces of a 28,000-year-old mammoth","authors":"Doris A. van Bergeijk, Hannah E. Augustijn, Somayah S. Elsayed, Joost Willemse, Victor J. Carrión, Chao Du, Mia Urem, Lena V. Grigoreva, Maksim Y. Cheprasov, Semyon Grigoriev, Hans Jansen, Bas Wintermans, Andries E. Budding, Herman P. Spaink, Marnix H. Medema, Gilles P. van Wezel","doi":"10.1111/1462-2920.16589","DOIUrl":"10.1111/1462-2920.16589","url":null,"abstract":"Ancient environmental samples, including permafrost soils and frozen animal remains, represent an archive with microbial communities that have barely been explored. This yet unexplored microbial world is a genetic resource that may provide us with new evolutionary insights into recent genomic changes, as well as novel metabolic pathways and chemistry. Here, we describe Actinomycetota Micromonospora, Oerskovia, Saccharopolyspora, Sanguibacter and Streptomyces species were successfully revived and their genome sequences resolved. Surprisingly, the genomes of these bacteria from an ancient source show a large phylogenetic distance to known strains and harbour many novel biosynthetic gene clusters that may well represent uncharacterised biosynthetic potential. Metabolic profiles of the strains display the production of known molecules like antimycin, conglobatin and macrotetrolides, but the majority of the mass features could not be dereplicated. Our work provides insights into Actinomycetota isolated from an ancient source, yielding unexplored genomic information that is not yet present in current databases.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":null,"pages":null},"PeriodicalIF":5.1,"publicationDate":"2024-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.16589","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139734771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acetic acid stress response of the acidophilic sulfate reducer Acididesulfobacillus acetoxydans 嗜酸性硫酸盐还原菌 Acididesulfobacillus acetoxydans 的醋酸应激反应。

IF 5.1 2区生物学

Environmental microbiology Pub Date : 2024-02-14 DOI: 10.1111/1462-2920.16565

Reinier A. Egas, Diana X. Sahonero-Canavesi, Nicole J. Bale, Michel Koenen, Çağlar Yildiz, Laura Villanueva, Diana Z. Sousa, Irene Sánchez-Andrea

{"title":"Acetic acid stress response of the acidophilic sulfate reducer Acididesulfobacillus acetoxydans","authors":"Reinier A. Egas, Diana X. Sahonero-Canavesi, Nicole J. Bale, Michel Koenen, Çağlar Yildiz, Laura Villanueva, Diana Z. Sousa, Irene Sánchez-Andrea","doi":"10.1111/1462-2920.16565","DOIUrl":"10.1111/1462-2920.16565","url":null,"abstract":"Acid mine drainage (AMD) waters are a severe environmental threat, due to their high metal content and low pH (pH <3). Current technologies treating AMD utilize neutrophilic sulfate-reducing microorganisms (SRMs), but acidophilic SRM could offer advantages. As AMDs are low in organics these processes require electron donor addition, which is often incompletely oxidized into organic acids (e.g., acetic acid). At low pH, acetic acid is undissociated and toxic to microorganisms. We investigated the stress response of the acetotrophic Acididesulfobacillus acetoxydans to acetic acid. A. acetoxydans was cultivated in bioreactors at pH 5.0 (optimum). For stress experiments, triplicate reactors were spiked until 7.5 mM of acetic acid and compared with (non-spiked) triplicate reactors for physiological, transcriptomic, and membrane lipid changes. After acetic acid spiking, the optical density initially dropped, followed by an adaptation phase during which growth resumed at a lower growth rate. Transcriptome analysis revealed a downregulation of genes involved in glutamate and aspartate synthesis following spiking. Membrane lipid analysis revealed a decrease in iso and anteiso fatty acid relative abundance; and an increase of acetyl-CoA as a fatty acid precursor. These adaptations allow A. acetoxydans to detoxify acetic acid, creating milder conditions for other microorganisms in AMD environments.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":null,"pages":null},"PeriodicalIF":5.1,"publicationDate":"2024-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.16565","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139734769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Viral communities in millipede guts: Insights into the diversity and potential role in modulating the microbiome 千足虫内脏中的病毒群落：洞察微生物组的多样性和潜在调节作用。

IF 5.1 2区生物学

Environmental microbiology Pub Date : 2024-02-14 DOI: 10.1111/1462-2920.16586

Julius Eyiuche Nweze, Johannes Sergej Schweichhart, Roey Angel

{"title":"Viral communities in millipede guts: Insights into the diversity and potential role in modulating the microbiome","authors":"Julius Eyiuche Nweze, Johannes Sergej Schweichhart, Roey Angel","doi":"10.1111/1462-2920.16586","DOIUrl":"10.1111/1462-2920.16586","url":null,"abstract":"Millipedes are important detritivores harbouring a diverse microbiome. Previous research focused on bacterial and archaeal diversity, while the virome remained neglected. We elucidated the DNA and RNA viral diversity in the hindguts of two model millipede species with distinct microbiomes: the tropical Epibolus pulchripes (methanogenic, dominated by Bacillota) and the temperate Glomeris connexa (non-methanogenic, dominated by Pseudomonadota). Based on metagenomic and metatranscriptomic assembled viral genomes, the viral communities differed markedly and preferentially infected the most abundant prokaryotic taxa. The majority of DNA viruses were Caudoviricetes (dsDNA), Cirlivirales (ssDNA) and Microviridae (ssDNA), while RNA viruses consisted of Leviviricetes (ssRNA), Potyviridae (ssRNA) and Eukaryotic viruses. A high abundance of subtypes I-C, I-B and II-C CRISPR-Cas systems was found, primarily from Pseudomonadota, Bacteroidota and Bacillota. In addition, auxiliary metabolic genes that modulate chitin degradation, vitamins and amino acid biosynthesis and sulphur metabolism were also detected. Lastly, we found low virus-to-microbe-ratios and a prevalence of lysogenic viruses, supporting a Piggyback-the-Winner dynamic in both hosts.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":null,"pages":null},"PeriodicalIF":5.1,"publicationDate":"2024-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.16586","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139734772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decoding the RNA virome of the tree parasite Armillaria provides new insights into the viral community of soil-borne fungi 解码树寄生虫阿米拉里亚的 RNA 病毒组为了解土传真菌的病毒群落提供了新的视角

IF 5.1 2区生物学

Environmental microbiology Pub Date : 2024-02-13 DOI: 10.1111/1462-2920.16583

Wajeeha Shamsi, Renate Heinzelmann, Sven Ulrich, Hideki Kondo, Carolina Cornejo

{"title":"Decoding the RNA virome of the tree parasite Armillaria provides new insights into the viral community of soil-borne fungi","authors":"Wajeeha Shamsi, Renate Heinzelmann, Sven Ulrich, Hideki Kondo, Carolina Cornejo","doi":"10.1111/1462-2920.16583","DOIUrl":"10.1111/1462-2920.16583","url":null,"abstract":"The globally distributed basidiomycete genus Armillaria includes wood decomposers that can act as opportunistic parasites, causing deadly root rot on woody plants. To test whether RNA viruses are involved in this opportunistic behaviour, a large isolate collection of five Armillaria species collected over 40 years in Switzerland from trees, dead wood and soil was analysed. De novo assembly of RNA-Seq data revealed 21 viruses, 14 of which belong to putative new species. Two dsRNA viruses and an unclassified Tymovirales are formally described for the first time for Armillaria. One mitovirus occurred with a high prevalence of 71.1%, while all other viruses were much less prevalent (0.6%–16.9%). About half of all viruses were found only in one fungal species, others occurred in 2–6 fungal species. Co-infections of 2–7 viruses per isolate were not uncommon (34.9%), and most viruses persisted circulating within fungal populations for decades. Some viruses were related to viruses associated with other Armillaria species, supporting the hypothesis that virus transmission can occur between different fungal species. Although no specific correlation between viruses and the fungal trophic strategy was found, this study opens new insights into viral diversity hidden in the soil microbiome.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":null,"pages":null},"PeriodicalIF":5.1,"publicationDate":"2024-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.16583","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139728262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbiology of Big Soda Lake, a multi-extreme meromictic volcanic crater lake in the Nevada desert 大苏打湖的微生物学--内华达沙漠中的一个多极端子母火山口湖

IF 5.1 2区生物学

Environmental microbiology Pub Date : 2024-02-13 DOI: 10.1111/1462-2920.16578

Hengameh H. Soufi, Daniel Tran, Stilianos Louca

{"title":"Microbiology of Big Soda Lake, a multi-extreme meromictic volcanic crater lake in the Nevada desert","authors":"Hengameh H. Soufi, Daniel Tran, Stilianos Louca","doi":"10.1111/1462-2920.16578","DOIUrl":"10.1111/1462-2920.16578","url":null,"abstract":"Big Soda Lake, Nevada, is a multi-extreme meromictic lake, whose hypersaline hyperalkaline bottom waters feature permanent anoxia and high concentrations of arsenic, sulphide and ammonia. These properties make Big Soda Lake—and the adjacent Little Soda Lake—a fascinating system for exploring life's boundaries, discovering novel microbial taxa and identifying biotechnologically useful strains. To date, the taxonomic diversity and metabolic capabilities of microorganisms in this system remain largely unknown. Here, we fill this gap using microbiome surveys across the Big and Little Soda Lake water columns, including 16S rRNA sequencing, fungal ITS2 sequencing and gene- and genome-resolved metagenomics. We accompany these surveys with measurements of salinity, pH, temperature, oxygen, ammonium and ammonia concentrations. Our analyses reveal rich bacterial communities, taxonomically and functionally differentiated along Big Soda Lake's oxycline and, to lesser extent, between lakes. Fungal communities were dominated by a small number of families, while nearly no archaea were detected. Pathways related to perchlorate reduction, anoxygenic phototrophy, fermentation, dissimilatory metabolism of arsenite/arsenate, sulphur compounds, nitrogen compounds and hydrogen, were particularly prevalent. A total of 129 high-quality bacterial and archaeal metagenome-assembled genomes (completeness ≥ 80%, contamination ≤ 5%) were recovered, yielding insight into the taxonomic distribution of microbial metabolic pathways.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":null,"pages":null},"PeriodicalIF":5.1,"publicationDate":"2024-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139728273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dissecting host-microbiome interaction effects on phytoplankton microbiome composition and diversity 剖析宿主-微生物组相互作用对浮游植物微生物组组成和多样性的影响

IF 5.1 2区生物学

Environmental microbiology Pub Date : 2024-02-13 DOI: 10.1111/1462-2920.16585

Jinny Wu Yang, Vincent J. Denef

{"title":"Dissecting host-microbiome interaction effects on phytoplankton microbiome composition and diversity","authors":"Jinny Wu Yang, Vincent J. Denef","doi":"10.1111/1462-2920.16585","DOIUrl":"10.1111/1462-2920.16585","url":null,"abstract":"Phytoplankton and their associated microbiomes of heterotrophic bacteria are foundational to primary production, energy transfer, and biogeochemical cycling in aquatic systems. While it is known that these microbiomes are shaped by host-released dissolved organic matter (DOM), the extent to which dynamic phytoplankton-bacteria interactions shape bacterial community assembly remains to be examined. Here, we investigated the effects of two mechanisms in host-microbiome interactions on phytoplankton bacterial microbiome formation: (i) innate host selection and (ii) host-microbiome feedback. For the former, phytoplankton-produced DOM composition is based solely on the host's properties (species or physiological state); for the latter, the presence of the microbiome modifies host DOM production. The microbiome of Chlorella sorokiniana was extracted and exposed to six ratios of the two effects. We found that microbiome composition changed along with the six host-microbiome feedback versus innate host selection ratios, with the highest compositional distance between communities under the strongest and the weakest ratio of the two effects. This indicates that each mechanism selects for different bacterial species. In addition, our findings showed that when both selective forces were applied, it led to a higher community richness, while host-microbiome feedback alone reduces community evenness due to its strong species-specific selection.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":null,"pages":null},"PeriodicalIF":5.1,"publicationDate":"2024-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.16585","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139728287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Archaeal blooms and busts in an estuarine time series 河口时间序列中的古生物繁荣与萧条

IF 5.1 2区生物学

Environmental microbiology Pub Date : 2024-02-07 DOI: 10.1111/1462-2920.16584

Justin T. Guider, Kristin M. Yoshimura, Kaleigh R. Block, Jennifer F. Biddle, Sunita R. Shah Walter

{"title":"Archaeal blooms and busts in an estuarine time series","authors":"Justin T. Guider, Kristin M. Yoshimura, Kaleigh R. Block, Jennifer F. Biddle, Sunita R. Shah Walter","doi":"10.1111/1462-2920.16584","DOIUrl":"https://doi.org/10.1111/1462-2920.16584","url":null,"abstract":"Coastal bays, such as Delaware Bay, are highly productive, ecologically important transitions between rivers and the coastal ocean. They offer opportunities to investigate archaeal assemblages across seasons, with the exchange of water masses that occurs with tidal cycles, and in the context of variable organic matter quality. For a year-long estuarine, size-fractionated time series, we used amplicon sequencing, chemical measurements, and qPCR to follow archaeal groups through the seasons. We detected seasonally high abundances of Marine Group II archaea in summer months which correlate with indicators of phytoplankton production, although not phytoplankton biomass. Although previous studies have reported associations between Marine Group II archaea and particles, here they are almost entirely found in very small particles (0.22–0.7 μm), suggesting they are free-living cells. Populations of Nitrososphaeria did not vary with particle size or environmental conditions. Methanogens were significant fractions of archaeal sequences in large particles at low tide during winter months. Contrary to expectations, Nanoarchaeia were found predominantly in the free-living fraction despite the previous observation that they require an association with hosts. These results underscore the utility of time series studies in shallow, tidally mixed estuarine environments that capture variable conditions for understanding the ecology and biogeochemistry of planktic archaea.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":null,"pages":null},"PeriodicalIF":5.1,"publicationDate":"2024-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139704681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0