Environmental microbiology最新文献

Patchy Distribution of Potato Cyst Nematodes Within Single Arable Fields Reveals Local Disease Suppressiveness Mediated by Disparate Microbial Communities 马铃薯囊肿线虫在单一耕地内的斑状分布揭示了不同微生物群落介导的局部疾病抑制作用

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-05-26 DOI: 10.1111/1462-2920.70113

Robbert van Himbeeck, Stefan Geisen, Casper van Schaik, Sven van den Elsen, Roeland Berendsen, André Bertran, Egbert Schepel, Johannes Helder

{"title":"Patchy Distribution of Potato Cyst Nematodes Within Single Arable Fields Reveals Local Disease Suppressiveness Mediated by Disparate Microbial Communities","authors":"Robbert van Himbeeck, Stefan Geisen, Casper van Schaik, Sven van den Elsen, Roeland Berendsen, André Bertran, Egbert Schepel, Johannes Helder","doi":"10.1111/1462-2920.70113","DOIUrl":"https://doi.org/10.1111/1462-2920.70113","url":null,"abstract":"Disease suppressiveness is a complex phenomenon that is assumed to be the resultant of the actions of local microbial antagonists. Exploitation of disease suppressiveness as a tool to manage pathogens is hindered by our poor understanding of this phenomenon. Here we investigated soil microbiome-based suppression of potato cyst nematodes (PCN), and to this end, four apparently homogeneous potato fields with an unexplained non-homogeneous PCN distribution were selected. We hypothesised that this patchy PCN distribution resulted from local variation in disease suppressiveness. Under controlled greenhouse conditions, we confirmed the overall suppressiveness of these soils vis-à-vis PCN and soils were gamma-irradiated to corroborate the biotic origin of this suppression. Subsequent DNA-based analysis of the microbial community in the potato rhizosphere revealed suppressiveness-related contrasts in community composition between suppressive and conducive patches. Elevated abundances of fungal (e.g., Metacordyceps chlamydosporia) and bacterial (e.g., Pseudomonas fluorescens) nematode antagonists were positively associated with PCN suppressive patches. Distinct sets of antagonists were found to be associated with PCN suppression despite the geographical closeness of the locations under investigation. Our findings confirm the biotic origin of local PCN suppressiveness and reveal that disparate microbial communities could achieve similar outcomes.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 5","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70113","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144140333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enteroviruses in Water: Epidemiology, Detection and Inactivation 水中肠病毒：流行病学、检测和灭活

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-05-19 DOI: 10.1111/1462-2920.70109

Corentin Morvan, Magloire P. Nekoua, Chaldam J. Mbani, Cyril Debuysschere, Enagnon K. Alidjinou, Didier Hober

引用次数: 0

Unearthing Vertical Stratified Archaeal Community and Associated Methane Metabolism in Thermokarst Sediments 热岩溶沉积物中垂直分层古细菌群落及其甲烷代谢

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-05-19 DOI: 10.1111/1462-2920.70110

Ze Ren, Mei Wang, Jinlei Yu, Lixiang Zhang, Zhenmei Lin, Xia Li, Yunlin Zhang

{"title":"Unearthing Vertical Stratified Archaeal Community and Associated Methane Metabolism in Thermokarst Sediments","authors":"Ze Ren, Mei Wang, Jinlei Yu, Lixiang Zhang, Zhenmei Lin, Xia Li, Yunlin Zhang","doi":"10.1111/1462-2920.70110","DOIUrl":"https://doi.org/10.1111/1462-2920.70110","url":null,"abstract":"<div>\u0000 \u0000 Thermokarst lakes are hotspots for greenhouse gas emissions across the Arctic and Qinghai-Tibet Plateau. Investigating the vertical stratification of archaeal communities in thermokarst lake sediments is essential for understanding their ecological roles and contributions to methane production. Here, we analysed archaeal communities along a depth gradient in thermokarst lake sediments. Alpha diversity (richness and Shannon index) generally decreased with depth. Euryarchaeota was the most abundant phylum, though its relative abundance declined with depth, while Thaumarchaeota increased. At the order level, Methanosarcinales and Nitrosopumilales showed increased relative abundance with depth, indicating adaptation to deeper anoxic layers, whereas Methanomicrobiales and Methanotrichales decreased. Beta diversity increased with depth, shifting from stochastic to deterministic processes. Network topology revealed reduced species connectivity but heightened modularity at depth, signalling niche specialisation. Functionally, genes associated with the initial steps of methane metabolism (Fwd, Mtd, Mer) increased with depth, while those involved in later steps (Mtr, Mcr) decreased, suggesting reduced energy conservation efficiency and lower overall methanogenesis rates in deeper sediments. These findings highlight the significant impact of vertical stratification on archaeal community structure, interaction networks, and functional capabilities.\u0000 </div>","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 5","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144091833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plant Genetic Bases Associated With Microbiota Descriptors Shed Light Into a Novel Holobiont Generalist Genes Theory 与微生物群描述符相关的植物遗传基础揭示了一种新的全生物通才基因理论。

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-05-15 DOI: 10.1111/1462-2920.70108

Loeiz Maillet, Manon Norest, Adam Kautsky, Anna Geraci, Elisabetta Oddo, Angelo Troia, Anne-Yvonne Guillerm-Erckelboudt, Cyril Falentin, Mathieu Rousseau-Gueutin, Anne-Marie Chèvre, Benjamin Istace, Corinne Cruaud, Caroline Belser, Jean-Marc Aury, Rosario Schicchi, Léa Frachon, Claudia Bartoli

{"title":"Plant Genetic Bases Associated With Microbiota Descriptors Shed Light Into a Novel Holobiont Generalist Genes Theory","authors":"Loeiz Maillet, Manon Norest, Adam Kautsky, Anna Geraci, Elisabetta Oddo, Angelo Troia, Anne-Yvonne Guillerm-Erckelboudt, Cyril Falentin, Mathieu Rousseau-Gueutin, Anne-Marie Chèvre, Benjamin Istace, Corinne Cruaud, Caroline Belser, Jean-Marc Aury, Rosario Schicchi, Léa Frachon, Claudia Bartoli","doi":"10.1111/1462-2920.70108","DOIUrl":"10.1111/1462-2920.70108","url":null,"abstract":"Plants as animals are associated with a cortege of microbes influencing their health, fitness and evolution. Scientists refer to all living organisms as holobionts, complex genetic units that coevolve simultaneously. This is what has been recently proposed as the hologenome theory. This exciting theory has important implications on animal and plant health; however, it still needs consistent proof to be validated. Indeed, holobionts are still poorly studied in their natural habitats where coevolution processes occur. Compared to animals, wild plant populations are an excellent model to explore the hologenome theory. These sessile holobionts have coevolved with their microbiota for decades, and natural selection and adaptive processes acting on wild plants are likely to regulate the plant–microbe interactions. Here, we conducted a microbiota survey, plant genome sequencing and genome–environmental analysis (GEA) of 26 natural populations of the plant species Brassica rapa. We collected plants over two seasons in Italy and France and analysed the root and rhizosphere microbiota. When conducting GEA, we evidenced neat peaks of association correlating with both fungal and bacterial microbiota. Surprisingly, we found 13 common genes between fungal and bacterial diversity descriptors that we referred to under the name of holobiont generalist genes (HGGs).","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 5","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70108","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144065806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Environmental Heterogeneity Drives Ecological Differentiation in Vibrio Populations Across Subtropical Marine Habitats

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-05-13 DOI: 10.1111/1462-2920.70107

Siu Hei Wan, Yangbing Xu, Wenqian Xu, Shara K. K. Leung, Erin Y. N. Yu, Charmaine C. M. Yung

{"title":"Environmental Heterogeneity Drives Ecological Differentiation in Vibrio Populations Across Subtropical Marine Habitats","authors":"Siu Hei Wan, Yangbing Xu, Wenqian Xu, Shara K. K. Leung, Erin Y. N. Yu, Charmaine C. M. Yung","doi":"10.1111/1462-2920.70107","DOIUrl":"https://doi.org/10.1111/1462-2920.70107","url":null,"abstract":"Elucidating how environmental gradients structure bacterial communities remains fundamental to microbial ecology. We investigated Vibrio population dynamics across contrasting subtropical marine environments in Hong Kong over a year period. Using an integrated approach combining cultivation techniques with molecular analyses of Hsp60 and 16S rRNA genes, we characterised the population structure between a coastal site (Clear Water Bay) and an estuarine site (Deep Bay). The estuarine environment consistently harboured higher Vibrio abundances (104–107 copies/mL) compared to coastal waters (102–104 copies/mL), with significantly greater phylogenetic diversity. Multivariate analyses revealed salinity as the primary driver of community differentiation between sites, while temperature governed seasonal succession patterns. Phylogenetic analysis of 1521 Vibrio isolates identified three distinct ecological groups corresponding to specific temperature-salinity niches, with evidence of habitat-specific thermal adaptations among closely related strains. Experimental characterisation of thermal performance curves confirmed physiological differentiation between warm- and cool-temperature adapted strains despite high genetic similarity (> 97% Hsp60 gene sequence identity). Several abundant species detected via amplicon sequencing (including V. navarrensis and V. mimicus) displayed site-specific ecotypes but remained uncultivated, highlighting methodological constraints in community characterisation. Our findings demonstrate how environmental heterogeneity drives fine-scale ecological differentiation in Vibrio populations, providing insights into mechanisms of bacterial adaptation in dynamic marine environments.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 5","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70107","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143944408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ampicillin Exposure and Glutathione Deficiency Synergistically Promote Conjugative Transfer of Plasmid-Borne Antibiotic Resistance Genes 氨苄西林暴露和谷胱甘肽缺乏协同促进质粒携带的抗生素抗性基因的共轭转移

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-05-10 DOI: 10.1111/1462-2920.70106

Shu-Yao Chen, Ke Huang, Ze-Hao He, Fang-Jie Zhao

{"title":"Ampicillin Exposure and Glutathione Deficiency Synergistically Promote Conjugative Transfer of Plasmid-Borne Antibiotic Resistance Genes","authors":"Shu-Yao Chen, Ke Huang, Ze-Hao He, Fang-Jie Zhao","doi":"10.1111/1462-2920.70106","DOIUrl":"https://doi.org/10.1111/1462-2920.70106","url":null,"abstract":"<div>\u0000 \u0000 Plasmid-mediated conjugation is an important pathway for the spread of antibiotic resistance genes (ARGs), posing a significant risk to global public health. It has been reported that the conjugative transfer of ARGs could be enhanced by oxidative stress. Whether endogenous glutathione (GSH), a major non-protein thiol compound involved in cellular redox homeostasis, influences conjugative transfer is unknown. In this study, we show that the deletion of the GSH biosynthesis gene gshA and ampicillin exposure synergistically promoted the conjugative transfer of plasmid RP4 bearing multiple ARGs from the soil bacterium Enterobacter sp. CZ-1 to Escherichia coli S17-1λπ in co-culture experiments and to diverse soil bacteria belonging to eight phyla, including some potential human pathogens, in a soil incubation experiment. The deletion of gshA increased ROS generation and cell membrane permeability, and upregulated the expression of the genes involved in intracellular oxidative stress regulation, membrane permeability, plasmid replication, and the SOS response process, especially under ampicillin exposure. These results suggest that endogenous GSH is an important factor affecting the spread of plasmid-borne ARGs. Exposure to antibiotics and environmental stresses that cause a depletion of endogenous GSH in vivo are likely to increase the risk of ARG dissemination in the environment.\u0000 </div>","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 5","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143930325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of Emerging Pathogens and Antibiotic Resistance Genes in the Biofilm of Microplastics Incubated Under a Wastewater Discharge Simulation 废水模拟培养微塑料生物膜中新发病原体和抗生素耐药基因的评估

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-05-09 DOI: 10.1111/1462-2920.70103

Stephanie A. Eytcheson, Sarah A. Brown, Huiyun Wu, Christopher T. Nietch, Paul C. Weaver, John A. Darling, Erik M. Pilgrim, S. Thomas Purucker, Marirosa Molina

{"title":"Assessment of Emerging Pathogens and Antibiotic Resistance Genes in the Biofilm of Microplastics Incubated Under a Wastewater Discharge Simulation","authors":"Stephanie A. Eytcheson, Sarah A. Brown, Huiyun Wu, Christopher T. Nietch, Paul C. Weaver, John A. Darling, Erik M. Pilgrim, S. Thomas Purucker, Marirosa Molina","doi":"10.1111/1462-2920.70103","DOIUrl":"https://doi.org/10.1111/1462-2920.70103","url":null,"abstract":"<div>\u0000 \u0000 Microplastics (MPs) are known vectors for the transport of pathogens and antibiotic resistance genes (ARGs), but few studies have examined the long-term (> 30 days) development of MP biofilms. Wastewater Treatment Plant (WWTP) effluents are a significant source of MPs, pathogens, and antibiotics released into the environment. We explored the development of biofilms on high- and low-density polyethylene, polypropylene, and polystyrene incubated in an experimental flow-through stream facility over the course of 10 weeks. Treatments included natural river water (RW) and RW amended with treated wastewater (TWW). Analysis of 16S rRNA amplicon sequencing results revealed that MPs in TWW and RW treatments developed distinct bacterial communities, displaying significant shifts in composition over time. Plastic type had only a minor effect influencing community composition after 10 weeks of incubation. The abundance of the sulfonamide resistance gene sulI, the mobile genetic element intI1, and the emerging pathogens Pseudomonas aeruginosa and Stenotrophomonas maltophilia increased significantly during the same time period. Our results indicate that as MPs persist and disperse in the environment, they may actively contribute to an increase in the risk of human exposure to ARGs and pathogens, especially if the system is impacted by wastewater treatment effluents.\u0000 </div>","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 5","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143925891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Environmental Microbiology and Environmental Microbiology Reports: Two Journals, One Goal 环境微生物学与环境微生物学报告：两种期刊，一个目标

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-05-06 DOI: 10.1111/1462-2920.70078

Gareth B. Jenkins, Eric S. Boyd, Antoine Danchin, Vincent Hervé, Megan J. Huggett, Álvaro Sánchez, Paul Trevorrow

引用次数: 0

Two Cold-Shock Proteins Characterised as RNA Chaperone of Hyperthermophilic Archaeon Pyrococcus yayanosii 超嗜热古细菌雅氏焦球菌RNA伴侣蛋白的两种冷休克蛋白

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-05-05 DOI: 10.1111/1462-2920.70105

Huanhuan Zhang, Zhe Gao, Cong Li, Jun Xu

{"title":"Two Cold-Shock Proteins Characterised as RNA Chaperone of Hyperthermophilic Archaeon Pyrococcus yayanosii","authors":"Huanhuan Zhang, Zhe Gao, Cong Li, Jun Xu","doi":"10.1111/1462-2920.70105","DOIUrl":"https://doi.org/10.1111/1462-2920.70105","url":null,"abstract":"<div>\u0000 \u0000 Cold shock proteins (Csps) play a crucial role in facilitating cellular growth at suboptimal temperatures. In this study, we identified and characterised two Csps, PyCsp and PyTRAM, in the hyperthermophilic archaeon Pyrococcus yayanosii A1. Using bio-layer interferometry (BLI) and molecular beacon assays, we demonstrated that both proteins exhibit RNA binding and unfolding activities in vitro. Heterologously expressed PyCsp and PyTRAM exhibited transcription anti-termination activity in Escherichia coli RL211 and could restore the growth of the cold-sensitive E. coli BX04 at 22°C. Knockout of the coding genes of either PyCsp or PyTRAM impaired the growth of P. yayanosii A1 at 85°C, a comparatively lower temperature to the optimal 95°C. Gene knockout and cross-complementation analyses of the coding genes for these two proteins suggest that PyCsp and PyTRAM functionally complement each other at low temperatures. PyTRAM contains the conserved TRAM domain, which is a typical characteristic of archaeal RNA chaperones. Notably, PyCsp shows low similarity to known archaeal RNA chaperones. Deletion of PYCH_0765, the gene encoding PyCsp, led to 27.5% changes in the transcriptome. This work highlights PyCsp as a non-TRAM class RNA chaperone that globally alters the transcriptome of P. yayanosii under cold shock conditions.\u0000 </div>","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 5","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143909168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid Colonisation of Plastic Surfaces by Marine Alcanivorax Bacteria Is Flagellum-Dependent and Influenced by Polymer Type and Photo-Weathering State 海洋Alcanivorax细菌对塑料表面的快速定植依赖于鞭毛，并受聚合物类型和光风化状态的影响

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-05-02 DOI: 10.1111/1462-2920.70102

Keren Davidov, Sheli Itzahri, Aiswarya Kartha, Gilad Orr, Ziv Lang, Shiri Navon-Venezia, Matan Oren

{"title":"Rapid Colonisation of Plastic Surfaces by Marine Alcanivorax Bacteria Is Flagellum-Dependent and Influenced by Polymer Type and Photo-Weathering State","authors":"Keren Davidov, Sheli Itzahri, Aiswarya Kartha, Gilad Orr, Ziv Lang, Shiri Navon-Venezia, Matan Oren","doi":"10.1111/1462-2920.70102","DOIUrl":"https://doi.org/10.1111/1462-2920.70102","url":null,"abstract":"Marine plastic debris provides stable surfaces for microbial colonisation, forming a unique ecosystem known as the plastisphere. Among the early colonisers are Alcanivorax bacteria, hydrocarbon degraders commonly found in oil-polluted seawater and on marine plastic surfaces. This study examined factors influencing the adhesion and colonisation dynamics of six Alcanivorax species. Flagellated species—A. balearicus, A. dieselolei and A. xenomutans—rapidly colonised plastics, particularly polyethylene and polypropylene, while non-flagellated species did not. Notably, plastic photo-weathering treatments led to the elongation of A. dieselolei cells, secretion of extracellular polymeric substance in some cases, and increased colonisation on UVB-treated polyethylene terephthalate. These changes may be linked to the reduced plastic surface hydrophobicity recorded following photo-weathering. To confirm the role of flagella in Alcanivorax adhesion, we disrupted flagellar activity using sub-concentrations of polymyxin B sulfate, resulting in inhibition of swarming motility and complete disruption of colonisation. These results contribute to our understanding of the interactions between hydrocarbon-degrading Alcanivorax bacteria and their plastic substrate, which in turn contributes to the understanding of the ecological impact of plastic pollution in marine environments.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 5","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70102","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143896847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0