Environmental microbiology最新文献

Confrontations of the Pathogenic Fungus Colletotrichum graminicola With a Biocontrol Bacterium or a Ubiquitous Fungus Trigger Synthesis of Secondary Metabolites With Lead Structures of Synthetic Fungicides 致病性真菌炭疽杆菌与生物防治菌或普遍存在的真菌的对抗引发合成杀菌剂含铅结构次级代谢物的合成

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-07-14 DOI: 10.1111/1462-2920.70145

Bennet Rohan Fernando Devasahayam, Henriette Uthe, Yvonne Poeschl, Holger B. Deising

{"title":"Confrontations of the Pathogenic Fungus Colletotrichum graminicola With a Biocontrol Bacterium or a Ubiquitous Fungus Trigger Synthesis of Secondary Metabolites With Lead Structures of Synthetic Fungicides","authors":"Bennet Rohan Fernando Devasahayam, Henriette Uthe, Yvonne Poeschl, Holger B. Deising","doi":"10.1111/1462-2920.70145","DOIUrl":"https://doi.org/10.1111/1462-2920.70145","url":null,"abstract":"Microbial biological control agents are increasingly used as an alternative to synthetic pesticides. The application of these microorganisms massively affects all members of plant-colonising microbial communities, including pathogenic fungi. In the majority of cases, the resulting competition for ecological niches is decided by the toxicity of microbial secondary metabolites (SMs) formed. In this study, we devised confrontation experiments employing the fungal maize pathogen Colletotrichum graminicola and antagonistic partners, that is the biocontrol bacterium Bacillus amyloliquefaciens and the ubiquitous ascomycete Aspergillus nidulans. Transcriptome studies uncovered strong de-regulation of the vast majority of the C. graminicola secondary metabolite biosynthetic gene clusters (SMBGCs), with 69% and 86% of these clusters de-regulated at confrontation sites with B. amyloliquefaciens or A. nidulans, respectively. In the biocontrol bacterium and in A. nidulans confronting the maize pathogen, 100% and 74% of the SMBGCs were transcriptionally de-regulated, respectively. Correspondingly, non-targeted high-resolution LC–MS/MS revealed a large repertoire of 1738 and 1466 novel features formed in the fungus–bacterium and fungus–fungus confrontation, respectively. Surprisingly, several of these belong to chemical classes with lead structures of synthetic fungicides.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 7","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70145","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144624610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling Crocosphaera Responses to Phosphorus Depletion: Insights From Genome Analysis and Functional Characterization 揭示鳄鱼对磷枯竭的反应：来自基因组分析和功能表征的见解

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-07-14 DOI: 10.1111/1462-2920.70153

Chloé Caille, Sophie Rabouille, Eva Ortega-Retuerta, Yann Denis, Olivier Crispi, Barbara Marie, Mireille Pujo-Pay, Vladimir Daric, Emmanuel Talla, Amel Latifi

{"title":"Unveiling Crocosphaera Responses to Phosphorus Depletion: Insights From Genome Analysis and Functional Characterization","authors":"Chloé Caille, Sophie Rabouille, Eva Ortega-Retuerta, Yann Denis, Olivier Crispi, Barbara Marie, Mireille Pujo-Pay, Vladimir Daric, Emmanuel Talla, Amel Latifi","doi":"10.1111/1462-2920.70153","DOIUrl":"https://doi.org/10.1111/1462-2920.70153","url":null,"abstract":"Unicellular, nitrogen-fixing cyanobacteria (UCYN) thrive and support primary production in oligotrophic oceans, playing a significant role in the marine nitrogen cycle. Crocosphaera sp., a model organism for studying marine nitrogen fixation, is adapted to low phosphate (Pi) concentrations. Yet, how Crocosphaera copes with Pi depletion is rather poorly understood. We present a genomics analysis of Pi stress-responsive genes in this genus, encompassing six C. watsonii and two strains isolated in coastal environments, C. subtropica and C. chwakensis. We identified genes involved in Pi signalling and uptake, and dissolved organic phosphorus (DOP) hydrolysis. Results showed different genetic potentials to cope with Pi scarcity between the Crocosphaera strains. Physiological monitoring of cultures of C. watsonii WH8501 exposed to Pi depletion highlighted a capacity to survive for at least nine days, albeit with a skewed C:N:P stoichiometry. Upon addition of DOP, cultures efficiently recovered to a growth rate and cell composition equivalent to those observed under favourable conditions. The concomitant transcription analysis revealed diel expression patterns of Pi-related genes and endogenous clock genes, suggesting a possible circadian regulation. Our data deepen our understanding of the growth strategies Crocosphaera employs in Pi-limited environments, offering broader insights into microbial resilience in marine ecosystems.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 7","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70153","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144624609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Herbicides Have Minimal and Variable Effects on the Structure and Function of Bacterial Communities in Agricultural Soils 除草剂对农业土壤细菌群落结构和功能的影响是微小的和可变的

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-07-14 DOI: 10.1111/1462-2920.70148

Margaret Gaylord, Abigail Thompson, Franck E. Dayan, Andrew R. Kniss, Dave Reichert, Kristen Otto, Rebecca Larson, Pankaj Trivedi

{"title":"Herbicides Have Minimal and Variable Effects on the Structure and Function of Bacterial Communities in Agricultural Soils","authors":"Margaret Gaylord, Abigail Thompson, Franck E. Dayan, Andrew R. Kniss, Dave Reichert, Kristen Otto, Rebecca Larson, Pankaj Trivedi","doi":"10.1111/1462-2920.70148","DOIUrl":"https://doi.org/10.1111/1462-2920.70148","url":null,"abstract":"Synthetic herbicides are relied on for weed management in the United States, and non-chemical alternatives, such as tillage, can be inefficient and expensive at an agricultural scale. Previous research examining the influence of weed management strategies on soil microbial communities has shown variability and inconsistency in experimental design and results. This study investigates the impact of repeated applications of glyphosate, a mixture of selective herbicides, and tillage on soil bacterial communities in sugar beet production across two locations in the United States. Using next-generation sequencing and various assays, we explore the effects on bacterial community structure, composition, and function related to nutrient cycling and soil health. Although transient and minor differences in bacterial community structure could be observed for herbicides and tillage, these statistical differences do not appear to be biologically relevant as the soil function was equivalent for all applied treatments. Our findings provide evidence that repeated herbicide usage does not directly impact soil health and function in sugar beet production. This study emphasises the need for well-replicated, field-realistic, and long-term experiments to understand the ecological consequences of herbicides and tillage in agroecosystems.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 7","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70148","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144624506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Species-Specific Exudates From the Harmful Algae Alexandrium minutum and Prymnesium parvum Drive the Structure of a Synthetic Microbial Community 有害藻类小亚历山大藻和小樱草藻的物种特异性分泌物驱动合成微生物群落的结构

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-07-11 DOI: 10.1111/1462-2920.70150

Enora Briand, Malwenn Lassudrie, Jean-Baptiste Bérard, Cécile Jauzein, Claire Labry, Thomas Lacour, Charlotte Nef, Cyril Noël, Virginie Raimbault, Nathalie Schreiber, Manoëlla Sibat, Simon Tanniou, Damien Réveillon, Matthieu Garnier

{"title":"Species-Specific Exudates From the Harmful Algae Alexandrium minutum and Prymnesium parvum Drive the Structure of a Synthetic Microbial Community","authors":"Enora Briand, Malwenn Lassudrie, Jean-Baptiste Bérard, Cécile Jauzein, Claire Labry, Thomas Lacour, Charlotte Nef, Cyril Noël, Virginie Raimbault, Nathalie Schreiber, Manoëlla Sibat, Simon Tanniou, Damien Réveillon, Matthieu Garnier","doi":"10.1111/1462-2920.70150","DOIUrl":"https://doi.org/10.1111/1462-2920.70150","url":null,"abstract":"<div>\u0000 \u0000 In this study, we examined the role of species-specific algal exudates in shaping bacterial community structure and interactions using synthetic microbial communities co-cultured with Alexandrium minutum and Prymnesium parvum. Cell-free algal exudates supported bacterial growth as the sole carbon source and revealed distinct exometabolomes unique to each algal species. These exometabolomes selectively influenced bacterial community composition, even among common copiotrophic taxa. Furthermore, co-culture experiments highlighted that the presence of algal cells drove further bacterial assembly, particularly within particle-attached communities, emphasising the role of close algal–bacterial interactions in structuring microbial consortia. Metabolomic analyses showed significant modulation of algal exudates by bacteria, with axenic cultures containing a broader and more diverse range of metabolites. This suggests microbial consumption, degradation or suppression of metabolite production under xenic conditions. Importantly, we observed a bacterial-mediated increase in extracellular paralytic shellfish toxins (PSTs) in A. minutum cultures. Exudates of both algal species displayed hemolytic activity, which was not affected by the presence of bacteria, suggesting bioactive extracellular compounds (BECs) production does not rely on bacteria. These findings underscore the complex and dynamic nature of algal–bacterial interactions, with implications for nutrient cycling, toxin dynamics and harmful algal bloom ecology.\u0000 </div>","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 7","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144606690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Methanotrophic Flexibility of ‘Ca. Methanoperedens’ and Its Interactions With Sulphate-Reducing Bacteria in the Sediment of Meromictic Lake Cadagno Ca的甲烷营养柔韧性。卡达尼奥分生湖沉积物中甲烷开孔菌及其与硫酸盐还原菌的相互作用

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-07-10 DOI: 10.1111/1462-2920.70133

Maider J. Echeveste Medrano, Guangyi Su, Lucas A. Blattner, Pedro Leão, Irene Sánchez-Andrea, Mike S. M. Jetten, Cornelia U. Welte, Jakob Zopfi

{"title":"Methanotrophic Flexibility of ‘Ca. Methanoperedens’ and Its Interactions With Sulphate-Reducing Bacteria in the Sediment of Meromictic Lake Cadagno","authors":"Maider J. Echeveste Medrano, Guangyi Su, Lucas A. Blattner, Pedro Leão, Irene Sánchez-Andrea, Mike S. M. Jetten, Cornelia U. Welte, Jakob Zopfi","doi":"10.1111/1462-2920.70133","DOIUrl":"https://doi.org/10.1111/1462-2920.70133","url":null,"abstract":"The greenhouse gas methane is an important contributor to global warming, with freshwater sediments representing important potential methane sources. Anaerobic methane-oxidising archaea mitigate methane release into the atmosphere by coupling the oxidation of methane to the reduction of extracellular electron acceptors or through interspecies electron transfer with microbial partners. Understanding their metabolic flexibility and microbial interactions is crucial to assess their role in global methane cycling. Here, we investigated anoxic sediments of the meromictic freshwater Lake Cadagno (Switzerland), where ‘Ca. Methanoperedens’ co-occur with a specific sulphate-reducing bacterium, with metagenomics and long-term incubations. Incubations were performed with different electron acceptors, revealing that manganese oxides supported highest CH4 oxidation potential but enriched for ‘Ca. Methanoperedens’ phylotypes that were hardly present in the inoculum. Combining data from the inoculum and incubations, we obtained five ‘Ca. Methanoperedens’ genomes, each harbouring different extracellular electron transfer pathways. In a reconstructed Desulfobacterota QYQD01 genome, we observed large multi-heme cytochromes, type IV pili, and a putative loss of hydrogenases, suggesting facultative syntrophic interactions with ‘Ca. Methanoperedens’. This research deepens our understanding of the metabolic flexibility and potential interspecific interactions of ‘Ca. Methanoperedens’ in freshwater lakes.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 7","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70133","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144598631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Wolbachia Feminises a Spider Host With Assistance From Co-Infecting Symbionts 沃尔巴克氏体在共感染共生体的帮助下使蜘蛛宿主雌性化

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-07-10 DOI: 10.1111/1462-2920.70149

Virginija Mackevicius-Dubickaja, Yuval Gottlieb, Jennifer A. White, Matthew R. Doremus

{"title":"Wolbachia Feminises a Spider Host With Assistance From Co-Infecting Symbionts","authors":"Virginija Mackevicius-Dubickaja, Yuval Gottlieb, Jennifer A. White, Matthew R. Doremus","doi":"10.1111/1462-2920.70149","DOIUrl":"https://doi.org/10.1111/1462-2920.70149","url":null,"abstract":"Arthropods commonly harbour maternally-transmitted bacterial symbionts that manipulate host biology. Multiple heritable symbionts can co-infect the same individual, allowing these host-restricted bacteria to engage in cooperation or conflict, which can ultimately affect host phenotype. The spider Mermessus fradeorum is infected with up to five heritable symbionts: Rickettsiella (R), Tisiphia (T), and three strains of Wolbachia (W1-3). Quintuply infected spiders are feminised, causing genetic males to develop as phenotypic females and produce almost exclusively female offspring. By comparing feminisation across nine infection combinations, we identified a Wolbachia strain, W1, that is required for feminisation. We also observed that spiders infected with both W1 and W3 produced ~10% more females than those lacking W3. This increase in feminisation rate does not seem to be due to direct changes in W1 titre, nor does W1 titre correlate with feminisation rate. Instead, we observed subtle titre interactions among symbionts, with lower relative abundance of R and T symbionts in strongly feminised infections. This synergistic effect of co-infection on Wolbachia feminisation may promote the spread of all five symbionts in spider populations. These results confirm the first instance of Wolbachia-induced feminisation in spiders and demonstrate that co-infecting symbionts can improve the efficacy of symbiont-induced feminisation.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 7","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70149","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144589681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to “Bacterial Diversity of Marine Biofilm Communities in Terra Nova Bay (Antarctica) by Culture-Dependent and -Independent Approaches” 更正“南极特拉诺瓦湾海洋生物膜群落细菌多样性的培养依赖和独立方法”

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-07-10 DOI: 10.1111/1462-2920.70135

引用次数: 0

Promotion of Fungal Bioactivity by Extracellular Polymeric Substances Under Phosphorus Deficiency 缺磷条件下胞外聚合物质对真菌生物活性的促进作用

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-07-08 DOI: 10.1111/1462-2920.70147

Shang Pan, Yizhou Jiang, Shihui Yan, Meiyue Xu, Yuyang Cao, Haoyang Weng, Zhijun Wang, Yixiao Shi, Zhen Li

{"title":"Promotion of Fungal Bioactivity by Extracellular Polymeric Substances Under Phosphorus Deficiency","authors":"Shang Pan, Yizhou Jiang, Shihui Yan, Meiyue Xu, Yuyang Cao, Haoyang Weng, Zhijun Wang, Yixiao Shi, Zhen Li","doi":"10.1111/1462-2920.70147","DOIUrl":"https://doi.org/10.1111/1462-2920.70147","url":null,"abstract":"<div>\u0000 \u0000 Extracellular polymeric substances (EPS) can work as a highly efficient nutrient source for many microorganisms. This study investigated the influences of EPS on the bioactivities of a typical phosphate-solubilising fungus, that is Aspergillus niger. The EPS were extracted from Rhodotorula mucilaginosa, with the addition of varying levels of inorganic phosphates. Under phosphorus (P) limitation, the cell wall thickness of A. niger increased from ~0.2 μm to ~0.4 μm. The addition of EPS relieved this phenomenon under the stress. In addition, it showed that EPS significantly promoted microbial growth with a four-fold increase in microbial biomass. For the treatment with the addition of sole inorganic phosphate, A. niger primarily secreted oxalic acid for phosphate solubilisation. However, for the treatment with sole EPS, the secretion of oxalic acid was inhibited, while the activity of acid phosphatase was promoted. Moreover, the concentration of oxalic acid was significantly reduced from approximately 90 to 1 mg/L during the co-existence of inorganic phosphate and EPS. Meanwhile, the activity of acid phosphatase was also enhanced. Therefore, A. niger can balance its strategy of P solubilisation, depending on the types of P sources. Furthermore, EPS was preferentially utilised over inorganic phosphate. Our findings highlight the potential of EPS for sustainable P supply.\u0000 </div>","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 7","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144582271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Eastern Joshua Tree Arbuscular Mycorrhizal Fungi Largely Consistent Across Roots, Soils, and Seasons 东部约书亚树丛枝菌根真菌在根、土壤和季节上基本一致

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-07-07 DOI: 10.1111/1462-2920.70138

Arik Joukhajian, Sydney I. Glassman

{"title":"Eastern Joshua Tree Arbuscular Mycorrhizal Fungi Largely Consistent Across Roots, Soils, and Seasons","authors":"Arik Joukhajian, Sydney I. Glassman","doi":"10.1111/1462-2920.70138","DOIUrl":"https://doi.org/10.1111/1462-2920.70138","url":null,"abstract":"The Mojave Desert is home to iconic Joshua trees threatened by climate change. Most desert plants form mutually beneficial partnerships with arbuscular mycorrhizal fungi (AMF), yet the AMF of the Eastern Joshua tree (Yucca jaegeriana) remain completely uncharacterized. We tested how Y. jaegeriana AMF spore abundance, richness, and composition varied when sampling 20 trees across 4 seasons from roots versus soils. We confirmed root colonisation via staining, assessed spore abundance via microscopy, and used Illumina MiSeq to sequence AMF virtual taxa (VT) with WANDA AML2 primers. We identified 12 spore morphotypes and 47 VTs across 5 families within Glomeromycotina, and the most abundant VT, Glomus VTX00294, appeared in 87% of soil and root samples. The majority of VTs (26/47) were present across all seasons and were shared among soil and roots (38/47), with more VTs unique to soil. In soil, per tree mean spore abundance and AMF richness were lowest in Summer but consistent across other seasons, with richness ranging from 8.8 to 11.5 VTs and mean root richness consistent across seasons. We conclude that sampling from soils rather than roots and any season other than Summer will yield the most diverse AMF communities.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 7","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70138","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144573767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extracellular Vesicles of a Phytobeneficial Bacterium Trigger Distinct Systemic Response in Plant 植物有益菌胞外囊泡在植物中引发不同的系统反应

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-07-07 DOI: 10.1111/1462-2920.70141

Timothée Zannis-Peyrot, Lucas Degusseau, Pierre-Yves Dugas, Fabiola Bastian, Matthieu Gaucher, Vincent Gaillard, Gilles Comte, Florence Wisniewski-Dye, Isabelle Kerzaon, Céline Lavire, Ludovic Vial

{"title":"Extracellular Vesicles of a Phytobeneficial Bacterium Trigger Distinct Systemic Response in Plant","authors":"Timothée Zannis-Peyrot, Lucas Degusseau, Pierre-Yves Dugas, Fabiola Bastian, Matthieu Gaucher, Vincent Gaillard, Gilles Comte, Florence Wisniewski-Dye, Isabelle Kerzaon, Céline Lavire, Ludovic Vial","doi":"10.1111/1462-2920.70141","DOIUrl":"https://doi.org/10.1111/1462-2920.70141","url":null,"abstract":"<div>\u0000 \u0000 Bacterial extracellular vesicles (EVs) are lipidic shuttles involved in inter-species communication, virulence, and host immune modulation. While their roles are increasingly understood in animal-bacteria interactions, knowledge of phytobacterial EVs remains limited. Recent findings indicate that biotic factors like hydroxycinnamic acids can regulate EV production. Hydroxycinnamic acids, such as ferulic acid, are abundant lignin components in the plant environment, influencing the ecology of numerous phytobacteria. Azospirillum sp. B510, a phytobeneficial bacterium, induces the accumulation of hydroxycinnamic acid derivatives in plants and can metabolise them. This study hypothesised that ferulic acid in the Azospirillum sp. B510 environment would influence EV production and, conversely, that these EVs would impact plant metabolites and defence gene expression. Our results demonstrate that ferulic acid influences the content of EVs released by Azospirillum sp. B510. Furthermore, bacterial EVs impact plant physiology at a systemic level according to their cargo. EVs induce systemic changes in Solanum lycopersicum metabolome, including alterations in hydroxycinnamic acid amide derivatives in roots and steroidal alkaloids in aerial tissues. Bacterial EVs also modulate tomato defence gene expression correlating with observed metabolite accumulation patterns. This research provides evidence of a global effect of bacterial EVs, highlighting the dynamic nature of plant-bacteria interactions mediated by EVs.\u0000 </div>","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 7","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144573768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0