Environmental microbiology最新文献

A 'Genome-First' Framework for Next-Generation Bioinputs: From Functional Mining to Rational Synthetic Microbial Communities. 下一代生物输入的“基因组优先”框架：从功能挖掘到合理的合成微生物群落。

IF 4 2区生物学

Environmental microbiology Pub Date : 2026-05-01 DOI: 10.1111/1462-2920.70319

Osiel Silva Gonçalves

{"title":"A 'Genome-First' Framework for Next-Generation Bioinputs: From Functional Mining to Rational Synthetic Microbial Communities.","authors":"Osiel Silva Gonçalves","doi":"10.1111/1462-2920.70319","DOIUrl":"10.1111/1462-2920.70319","url":null,"abstract":"The demand for sustainable agriculture has shifted bioprospecting towards microbial bioinputs as alternatives to chemical fertilisers and pesticides. Whole-genome sequencing accelerates the discovery of plant-growth-promoting bacteria (PGPB) by enabling the identification of functional genes and the prediction of traits such as nutrient solubilisation, phytohormone production and biocontrol. Traditionally a secondary tool for strain characterisation, genomics has evolved into a 'genome-first' strategy that effectively collapses the phenotypic bottleneck in prospective bioprospecting and the rational design of synthetic microbial communities (SynComs). In this review, we argue for a transition from empirical phenotypic screening towards a genomics-guided paradigm for the selection of next-generation bioinputs. This work demonstrates how actionable insights can be gained through the integration of high-resolution genome mining into discovery pipelines. We explore the application of reverse ecology to infer ecological roles from genomic content and emphasise the critical role of pangenomics in identifying traits linked to host colonisation and niche adaptation. Furthermore, we advocate for biosafety screening as a non-negotiable prerequisite for bioinoculant development to ensure ecological and clinical safety. Finally, this work proposes that genome-scale metabolic networks are essential to enable the transition from single-strain inoculants to the assembly of stable SynComs. This framework establishes a comprehensive, data-driven approach to predictable interventions in the agricultural bioeconomy.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"28 5","pages":"e70319"},"PeriodicalIF":4.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13143410/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147835567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Native Aquatic Plastispheres in a River-Wastewater Catchment: Carbapenem-Resistant Bacteria Isolation and Microscopy-Based Structural Analysis. 河流污水集水区的原生水生塑料球：碳青霉烯抗性细菌的分离和基于显微镜的结构分析。

IF 4 2区生物学

Environmental microbiology Pub Date : 2026-05-01 DOI: 10.1111/1462-2920.70312

Rolbiecki Damian, Jaszczyszyn Katarzyna, Wawrocki Sebastian, Jóźwiak Piotr, Gajewska Joanna, Czatzkowska Małgorzata, Harnisz Monika, Kiedrzyńska Edyta

{"title":"Native Aquatic Plastispheres in a River-Wastewater Catchment: Carbapenem-Resistant Bacteria Isolation and Microscopy-Based Structural Analysis.","authors":"Rolbiecki Damian, Jaszczyszyn Katarzyna, Wawrocki Sebastian, Jóźwiak Piotr, Gajewska Joanna, Czatzkowska Małgorzata, Harnisz Monika, Kiedrzyńska Edyta","doi":"10.1111/1462-2920.70312","DOIUrl":"10.1111/1462-2920.70312","url":null,"abstract":"Plastispheres, microbial biofilms formed on plastic surfaces, are increasingly recognised as ecological niches capable of transporting pollutants and antibiotic-resistant microorganisms. However, mechanistic insights into antimicrobial resistance (AMR) dynamics in natural plastispheres remain limited, particularly for priority pathogens such as carbapenem-resistant Enterobacterales (CRE). Here, we evaluated plastispheres as environmental reservoirs and vectors of carbapenem-resistant bacteria, comparing wastewater (secondary settling tanks, representing the final stage before environmental discharge) and riverine environments. Using a combined SEM-CFM approach, we resolved plastic surface topography and the spatial organisation of biofilm-associated bacteria. Although CRE were not detected, carbapenem-resistant bacteria constituted a stable fraction of heterotrophic communities in both environments and were primarily associated with intrinsic resistance mechanisms. Carbapenem-resistant isolates included Aeromonas spp. (blaCphA), Stenotrophomonas maltophilia (blaL1), and Pseudomonas putida (efflux-based resistance). Microscopy revealed dense bacterial clusters on plastic surfaces, suggesting microenvironments that may facilitate cell-cell interactions, including horizontal gene transfer. These findings highlight plastispheres not only as vectors of AMR but also as potential evolutionary hotspots shaping resistance persistence and dissemination in aquatic systems. Future integrating metagenomic and genomic data on resistance gene mobility with spatially resolved microbial community structure will provide critical insights into the mechanisms and risks of AMR dissemination in plastisphere environments.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"28 5","pages":"e70312"},"PeriodicalIF":4.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147766315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Flush With Data (or) Optimizing and Validating the Efficacy of Free and Computationally Simple 16S Metabarcoding Approaches for Use in Wastewater Surveillance. 用数据冲洗（或）优化和验证用于废水监测的免费和计算简单的16S元条形码方法的有效性。

IF 4 2区生物学

Environmental microbiology Pub Date : 2026-05-01 DOI: 10.1111/1462-2920.70276

Joe Berta, Lori A Rowe, Evan Multala, Robert F Garry

{"title":"Flush With Data (or) Optimizing and Validating the Efficacy of Free and Computationally Simple 16S Metabarcoding Approaches for Use in Wastewater Surveillance.","authors":"Joe Berta, Lori A Rowe, Evan Multala, Robert F Garry","doi":"10.1111/1462-2920.70276","DOIUrl":"10.1111/1462-2920.70276","url":null,"abstract":"We propose free and low-computationally complex methods of 16S rRNA metabarcoding analysis, then optimized and validate their accuracy for wastewater bacterial surveillance. Three taxonomic analysis pipelines were augmented: NCBI BLAST subsampling, Kraken 2/Bracken and QIIME 2/DADA 2. Our optimization strategies for the high complexity of wastewater samples raised QIIME 2/DADA 2's sensitivity to species-level taxa by 240.5%, while they increased the species-level selectivity of Kraken 2/Bracken and NCBI BLAST subsampling by 18.7% and 79.1%, respectively. Optimization vastly lowered the read mapping error for BLAST subsampling and Kraken 2/Bracken, by 42.0% and 11.4%, respectively. Microbial community diversity estimates were also improved through our optimization strategies. Richness measurements for BLAST subsampling became 95.6% more accurate, while Kraken 2/Bracken and QIIME 2/DADA 2 improved by 2.2% and 37.8%. Shannon entropy estimates by BLAST subsampling increased in accuracy by 17.4%, while for Kraken 2/Bracken and QIIME 2/DADA 2 they increased by 19.7% and 41.4%. For beta diversity, Bray-Curtis dissimilarity estimates by QIIME 2/DADA 2 increased in accuracy by 8.5% and by Kraken 2/Bracken by 174.3%.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"28 5","pages":"e70276"},"PeriodicalIF":4.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13130369/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147766259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Novel Glutamine Riboswitch, LRN, Regulates the Expression of a Nucleoside Permease in Bacillus thuringiensis. 一种新的谷氨酰胺核糖开关，LRN，调节苏云金芽孢杆菌核苷渗透酶的表达。

IF 4 2区生物学

Environmental microbiology Pub Date : 2026-05-01 DOI: 10.1111/1462-2920.70318

Jiaxin Qin, Yizhuo Zhang, Cheng Qian, Xia Cai, Bing Yan, Jun Cai

{"title":"A Novel Glutamine Riboswitch, LRN, Regulates the Expression of a Nucleoside Permease in Bacillus thuringiensis.","authors":"Jiaxin Qin, Yizhuo Zhang, Cheng Qian, Xia Cai, Bing Yan, Jun Cai","doi":"10.1111/1462-2920.70318","DOIUrl":"https://doi.org/10.1111/1462-2920.70318","url":null,"abstract":"Knowledge of the regulatory mechanism of riboswitches is vital for understanding how microorganisms cope with changes in both intracellular and extracellular environments and for developing and applying RNA biosensors. To date, two types of glutamine-based riboswitches, which are exclusively distributed in cyanobacteria, have been identified. Here, we found an RNA regulatory element in the 5'UTR of the nucleoside permease gene (nupC) in Bacillus thuringensis BMB171; it was identified as a novel glutamine riboswitch and named LRN (leader RNA of nupC). Unlike the two previously known types of glutamine riboswitches found in cyanobacteria, LRN is a single-domain RNA element representing a novel type III glutamine riboswitch. Binding glutamine leads to rearrangements the LRN RNA structure, which inhibits downstream gene expression at the transcriptional level. Biocomputational searches revealed that LRN is frequently found in the Bacillus cereus group and is located mainly upstream of the coding region of the nupC homologues. Thus, this RNA-based sensing mechanism establishes a regulatory feedback loop that couples intracellular glutamine levels to nucleoside transport, which is shared by the B. cereus group.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"28 5","pages":"e70318"},"PeriodicalIF":4.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147835553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pandemic Vibrio cholerae and the Environmental Reservoir Hypothesis-Outstanding Questions Central to Global Cholera Control. 大流行霍乱弧菌和环境水库假说-全球霍乱控制中心悬而未决的问题。

IF 4 2区生物学

Environmental microbiology Pub Date : 2026-05-01 DOI: 10.1111/1462-2920.70302

Azfar D Hossain, William P Robins, John J Mekalanos, Louise C Ivers

引用次数: 0

Protist Diversity in La Union, Philippines Revealed by Metabarcoding: Ecosystem- and Database-Dependent Lineage Patterns. 元条形码揭示的菲律宾La Union原生生物多样性：生态系统和数据库依赖的谱系模式。

IF 4 2区生物学

Environmental microbiology Pub Date : 2026-05-01 DOI: 10.1111/1462-2920.70321

Christon Jairus M Racoma, Nikki Heherson A Dagamac, Priyal Patel, Saron Ghebrezadik, Yonas Isaak Tekle

{"title":"Protist Diversity in La Union, Philippines Revealed by Metabarcoding: Ecosystem- and Database-Dependent Lineage Patterns.","authors":"Christon Jairus M Racoma, Nikki Heherson A Dagamac, Priyal Patel, Saron Ghebrezadik, Yonas Isaak Tekle","doi":"10.1111/1462-2920.70321","DOIUrl":"https://doi.org/10.1111/1462-2920.70321","url":null,"abstract":"Protists are widely distributed, single-celled eukaryotes whose hidden diversity has increasingly been revealed through molecular techniques. Despite this progress, global studies remain uneven, with Southeast Asia, particularly the Philippines, understudied. This research investigates protist community diversity, including Amoebozoa as a focal subgroup, across freshwater and marine ecosystems using a metabarcoding approach supported by two reference databases. Environmental DNA was collected from sites, and the V4 region of the 18S rRNA gene was amplified and sequenced using Oxford Nanopore Technologies. Results showed that the Telonemia, Stramenopiles, Alveolata, and Rhizaria (TSAR) supergroup consistently dominated protist communities across ecosystems. Richness curves approached saturation, with freshwater habitats exhibiting broader family level diversity and generally higher taxonomic richness. While community composition differed between freshwater and marine environments, multivariate analyses revealed partial overlap, with patterns influenced by the choice of reference database. Marine communities were more compositionally heterogeneous, whereas Amoebozoa displayed relatively stable but low abundance distributions. Although database selection strongly affected inferred taxonomic composition and reduced overlap between datasets, the ecological gradient between ecosystems remained consistent. These findings demonstrate that metabarcoding captures broad ecological patterns in protist biogeography while highlighting the need for improved reference databases and further exploration of microbial diversity in the Philippines.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"28 5","pages":"e70321"},"PeriodicalIF":4.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147856184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Dairy Ruminant Gut Microbiome: Profile, Responsiveness to Seasonality and Impact on Milk Quality. 乳反刍动物肠道微生物群：特征、对季节性的反应和对牛奶品质的影响。

IF 4 2区生物学

Environmental microbiology Pub Date : 2026-05-01 DOI: 10.1111/1462-2920.70317

Alexandra Ntemiri, Adamantini Kyriacou, Myrsini Kakagianni

{"title":"The Dairy Ruminant Gut Microbiome: Profile, Responsiveness to Seasonality and Impact on Milk Quality.","authors":"Alexandra Ntemiri, Adamantini Kyriacou, Myrsini Kakagianni","doi":"10.1111/1462-2920.70317","DOIUrl":"https://doi.org/10.1111/1462-2920.70317","url":null,"abstract":"The gut microbiome (GM) and particularly the rumen microbiome (RM) affect the ruminant health and they are associated with milk quality and safety. The bovine RM has been extensively studied due to the importance of cattle in Agriculture. Studies of the gastrointestinal microbiome (GITM) of other livestock ruminants like goats, lag behind, comparatively. Sheep and goat rearing sustains livelihoods in unprivileged areas, globally. Dominant ruminant GM shaping factors are host and diet. Exposure to seasonal stimuli (e.g., diet) is associated with livestock GITM shifts. These seasonal microbial changes correlate with milk production and milk microbiota (MM) variation, with mechanistic links to remain unclear. The seasonality impact could be intensified during extensive rearing. Goats are frequently described as resilient livestock due to behavioural and dietary adaptability. If the goat GM responsiveness to seasonal stimuli plays a functional role in the livestock resilience and milk quality remains unresolved. Goats are discussed here as a comparatively underexplored system for investigating microbiome-mediated adaptation to variable environments. Here, we overviewed the current knowledge on the ruminant GM with emphasis to goats, and how seasonality may affect its configuration, and explored evidence linking seasonal GM variation to MM, milk quality and safety.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"28 5","pages":"e70317"},"PeriodicalIF":4.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13133252/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147812708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biodiversity, Distribution and Ecological Interactions of Algal-Bacterial Biofilms in Wastewater Treatment Systems. 污水处理系统中藻-细菌生物膜的生物多样性、分布和生态相互作用。

IF 4 2区生物学

Environmental microbiology Pub Date : 2026-05-01 DOI: 10.1111/1462-2920.70303

Abigail Lin, Sayanti Ghosh, Marek Kirs, Zhiyue Wang

{"title":"Biodiversity, Distribution and Ecological Interactions of Algal-Bacterial Biofilms in Wastewater Treatment Systems.","authors":"Abigail Lin, Sayanti Ghosh, Marek Kirs, Zhiyue Wang","doi":"10.1111/1462-2920.70303","DOIUrl":"https://doi.org/10.1111/1462-2920.70303","url":null,"abstract":"Nutrient contamination from wastewater threatens aquatic ecosystems by promoting harmful algal blooms and creating hypoxic dead zones. In nutrient-rich systems, algae uptake nitrogen and phosphorus while bacteria carry out nitrification and denitrification. Although algae and bacteria have been employed together to enhance nutrient removal processes in wastewater treatment systems, there is limited understanding of specific algal-bacterial interactions, roles of macroalgae and biodiversity of non-suspended microbes. In this study, next-generation sequencing of the V4 region of 16S rRNA and the V8-V9 region of 18S rRNA was used to examine the biodiversity of biofilm communities at wastewater treatment plants and the influence of water quality and algal-bacterial interactions on dominant microbes. Redundancy analysis revealed that the dominant algae genera strongly influenced by nitrogen and phosphorus content were Neochloris, Characiopodium, Ulva and Cladophora. Positive co-occurrence was primarily observed between algae and plant growth-promoting rhizobacteria (PGPR), namely Prototheca-Blastopirellula and Cladophora-Gordonia. Conversely, algae formed negative co-occurrence relationships with both PGPR (Characiopodium-Pseudomonas, Neochloris-Pseudomonas, Characiopodium-Acinetobacter) and gut microbes (Neochloris-Christensenellaceae R-7, Ulva-Christensenellaceae R-7, Cladophora-Christensenellaceae R-7, Ulva-Desulfovibrio). These results highlight the significance of macroalgae in biofilm communities and suggest potential algae-bacteria pairs for enhanced nutrient removal in on-site wastewater treatment.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"28 5","pages":"e70303"},"PeriodicalIF":4.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147835586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Atmospheric Dispersal and Local Drivers Shape Snow and Air Microbial Communities in the Western Antarctic Peninsula. 大气扩散和局部驱动因素影响南极半岛西部的雪和空气微生物群落。

IF 4 2区生物学

Environmental microbiology Pub Date : 2026-05-01 DOI: 10.1111/1462-2920.70316

María E Alcamán-Arias, Ignacio Ramos-Tapia, Francisco Fuentes, Roberto Bastías, Carlos A Manzano, Nicolás Plaza, Gastón Higuera, Daniel Araneda, Macarena Troncoso, Juan A Ugalde, Konstanza Vergara

{"title":"Atmospheric Dispersal and Local Drivers Shape Snow and Air Microbial Communities in the Western Antarctic Peninsula.","authors":"María E Alcamán-Arias, Ignacio Ramos-Tapia, Francisco Fuentes, Roberto Bastías, Carlos A Manzano, Nicolás Plaza, Gastón Higuera, Daniel Araneda, Macarena Troncoso, Juan A Ugalde, Konstanza Vergara","doi":"10.1111/1462-2920.70316","DOIUrl":"https://doi.org/10.1111/1462-2920.70316","url":null,"abstract":"Microbial communities in Antarctica's snow and atmosphere are critical for nutrient cycling and are highly sensitive to environmental change. We characterised the bacterial composition, diversity, and co-occurrence network structure of surface snow and airborne microbiomes from two contrasting bays along the Western Antarctic Peninsula (WAP): Arturo Prat Base (APB; Chile Bay, Greenwich Island) and Yelcho Base (YB; South Bay, Doumer Island), sampled during consecutive austral summers (2022-2023). Surface snow and air samples were analysed using 16S rRNA gene sequencing, diversity indices, and network analyses to evaluate spatial and interannual dynamics. Proteobacteria and Bacteroidota dominated both environments, but community structures differed between sites: APB showed stronger local and anthropogenic signals near logistic stations, whereas YB reflected more stable deposition regimes and distant atmospheric inputs. Airborne communities displayed limited interannual variation and high compositional similarity between sites, consistent with atmospheric seeding by polar air masses. Co-occurrence networks revealed denser, more structured interactions within snow communities, with keystone taxa linking snow and air, sustaining deposition-resuspension dynamics. These findings highlight that long-range atmospheric transport and local conditions shape Antarctic microbial dynamics, providing insights for forecasting microbiome responses and assessing airborne health risks amid human activity and climate change in polar regions.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"28 5","pages":"e70316"},"PeriodicalIF":4.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147835622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Core Microbial Taxa Strengthen Root Microbial Network Stability Under Drought Stress. 干旱胁迫下核心微生物类群增强根系微生物网络稳定性

IF 4 2区生物学

Environmental microbiology Pub Date : 2026-05-01 DOI: 10.1111/1462-2920.70307

Keren Wu, Hang-Wei Hu, Dorin Gupta, Yuan Li, Zi-Yang He, Feng Wang, Ji-Zheng He

{"title":"Core Microbial Taxa Strengthen Root Microbial Network Stability Under Drought Stress.","authors":"Keren Wu, Hang-Wei Hu, Dorin Gupta, Yuan Li, Zi-Yang He, Feng Wang, Ji-Zheng He","doi":"10.1111/1462-2920.70307","DOIUrl":"10.1111/1462-2920.70307","url":null,"abstract":"Drought stress is intensifying globally, but its effects on plant-associated microbiome diversity and stability remain poorly understood. We grew wheat under drought stress and sampled bulk soils, rhizosphere soils and roots across three growth stages to quantify microbial diversity, co-occurrence network stability and the contributions of core taxa to network stability. Drought affected microbial diversity depending on microbial kingdoms, plant niches and growth stages. We further found that drought stress reduced the complexity and stability of microbial networks in the rhizosphere soils while enhancing those in the roots, mainly through shifts in the abundances of core taxa (i.e., taxa that are widely distributed across samples, specific to drought stress and highly connected in the network). Three types of analyses (shared operational taxonomic units, network keystone nodes and taxa with high specificity and occupancy values) were employed to identify the core taxa enriched in the roots under drought stress, including Glycomyces and Thermoactinomycetaceae, which were typical drought-tolerant taxa that are important for maintaining root microbial network stability. Environment stress usually disrupts microbial community stability, but we found drought stress enriched core taxa, enhancing drought-tolerant crop root microbiomes stability. Our findings provide a blueprint for enhancing crop stress tolerance via microbiome manipulation.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"28 5","pages":"e70307"},"PeriodicalIF":4.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13128541/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147766349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0