Environmental microbiology最新文献

Two Cold-Shock Proteins Characterised as RNA Chaperone of Hyperthermophilic Archaeon Pyrococcus yayanosii 超嗜热古细菌雅氏焦球菌RNA伴侣蛋白的两种冷休克蛋白

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-05-05 DOI: 10.1111/1462-2920.70105

Huanhuan Zhang, Zhe Gao, Cong Li, Jun Xu

{"title":"Two Cold-Shock Proteins Characterised as RNA Chaperone of Hyperthermophilic Archaeon Pyrococcus yayanosii","authors":"Huanhuan Zhang, Zhe Gao, Cong Li, Jun Xu","doi":"10.1111/1462-2920.70105","DOIUrl":"https://doi.org/10.1111/1462-2920.70105","url":null,"abstract":"<div>\u0000 \u0000 Cold shock proteins (Csps) play a crucial role in facilitating cellular growth at suboptimal temperatures. In this study, we identified and characterised two Csps, PyCsp and PyTRAM, in the hyperthermophilic archaeon Pyrococcus yayanosii A1. Using bio-layer interferometry (BLI) and molecular beacon assays, we demonstrated that both proteins exhibit RNA binding and unfolding activities in vitro. Heterologously expressed PyCsp and PyTRAM exhibited transcription anti-termination activity in Escherichia coli RL211 and could restore the growth of the cold-sensitive E. coli BX04 at 22°C. Knockout of the coding genes of either PyCsp or PyTRAM impaired the growth of P. yayanosii A1 at 85°C, a comparatively lower temperature to the optimal 95°C. Gene knockout and cross-complementation analyses of the coding genes for these two proteins suggest that PyCsp and PyTRAM functionally complement each other at low temperatures. PyTRAM contains the conserved TRAM domain, which is a typical characteristic of archaeal RNA chaperones. Notably, PyCsp shows low similarity to known archaeal RNA chaperones. Deletion of PYCH_0765, the gene encoding PyCsp, led to 27.5% changes in the transcriptome. This work highlights PyCsp as a non-TRAM class RNA chaperone that globally alters the transcriptome of P. yayanosii under cold shock conditions.\u0000 </div>","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 5","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143909168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid Colonisation of Plastic Surfaces by Marine Alcanivorax Bacteria Is Flagellum-Dependent and Influenced by Polymer Type and Photo-Weathering State 海洋Alcanivorax细菌对塑料表面的快速定植依赖于鞭毛，并受聚合物类型和光风化状态的影响

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-05-02 DOI: 10.1111/1462-2920.70102

Keren Davidov, Sheli Itzahri, Aiswarya Kartha, Gilad Orr, Ziv Lang, Shiri Navon-Venezia, Matan Oren

{"title":"Rapid Colonisation of Plastic Surfaces by Marine Alcanivorax Bacteria Is Flagellum-Dependent and Influenced by Polymer Type and Photo-Weathering State","authors":"Keren Davidov, Sheli Itzahri, Aiswarya Kartha, Gilad Orr, Ziv Lang, Shiri Navon-Venezia, Matan Oren","doi":"10.1111/1462-2920.70102","DOIUrl":"https://doi.org/10.1111/1462-2920.70102","url":null,"abstract":"Marine plastic debris provides stable surfaces for microbial colonisation, forming a unique ecosystem known as the plastisphere. Among the early colonisers are Alcanivorax bacteria, hydrocarbon degraders commonly found in oil-polluted seawater and on marine plastic surfaces. This study examined factors influencing the adhesion and colonisation dynamics of six Alcanivorax species. Flagellated species—A. balearicus, A. dieselolei and A. xenomutans—rapidly colonised plastics, particularly polyethylene and polypropylene, while non-flagellated species did not. Notably, plastic photo-weathering treatments led to the elongation of A. dieselolei cells, secretion of extracellular polymeric substance in some cases, and increased colonisation on UVB-treated polyethylene terephthalate. These changes may be linked to the reduced plastic surface hydrophobicity recorded following photo-weathering. To confirm the role of flagella in Alcanivorax adhesion, we disrupted flagellar activity using sub-concentrations of polymyxin B sulfate, resulting in inhibition of swarming motility and complete disruption of colonisation. These results contribute to our understanding of the interactions between hydrocarbon-degrading Alcanivorax bacteria and their plastic substrate, which in turn contributes to the understanding of the ecological impact of plastic pollution in marine environments.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 5","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70102","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143896847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Towards a Stable Host–Parasite Relationship Between Honey Bees and Varroa Mites Through Innovative Beekeeping 通过创新养蜂方法建立蜜蜂与瓦螨稳定的寄主-寄生虫关系

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-05-01 DOI: 10.1111/1462-2920.70101

Lioba Hilsmann, Lena Wolf, Markus Thamm, Sylvie Vandenabeele, Ricarda Scheiner

{"title":"Towards a Stable Host–Parasite Relationship Between Honey Bees and Varroa Mites Through Innovative Beekeeping","authors":"Lioba Hilsmann, Lena Wolf, Markus Thamm, Sylvie Vandenabeele, Ricarda Scheiner","doi":"10.1111/1462-2920.70101","DOIUrl":"https://doi.org/10.1111/1462-2920.70101","url":null,"abstract":"Varroa destructor is a major factor in declining honey bee health worldwide. Conventional beekeeping involves multiple Varroa treatments, limiting bees' ability to adapt to the mite. To foster a stable host–parasite relationship, we tested an “innovative” beekeeping method with fewer Varroa treatments, focusing on its impact on honey bee health. We compared Varroa mite fall, immune responses, and seasonal dynamics of Deformed Wing Virus-B (DWV-B) in colonies managed under conventional and innovative practices. Viral loads of newly emerged honey bees and foragers were quantified three times during the season. Varroa mite fall was monitored and immune responses were assessed. In spring, bees managed with the innovative method had significantly lower haemocyte counts 48 h after emergence. DWV-B loads did not differ between groups in spring but were higher in summer in bees managed with the innovative method. After summer treatment, DWV-B loads and Varroa mite fall were similar between groups. Despite higher numbers in Varroa mite fall and DWV-B loads in summer, the innovative method reduced both by fall, ensuring healthy winter bee production and colony survival. These findings suggest that reducing Varroa treatments can support a stable host–parasite relationship while minimising negative effects on honey bee health.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 5","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70101","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143897147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Is the Gelatinous Matrix of Nassellaria (Radiolaria) a Strategy for Coping With Oligotrophy? 放射菌的凝胶基质是一种应对少营养的策略吗？

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-05-01 DOI: 10.1111/1462-2920.70098

Natalia Llopis Monferrer, Sarah Romac, Manon Laget, Yasuhide Nakamura, Tristan Biard, Miguel M. Sandin

{"title":"Is the Gelatinous Matrix of Nassellaria (Radiolaria) a Strategy for Coping With Oligotrophy?","authors":"Natalia Llopis Monferrer, Sarah Romac, Manon Laget, Yasuhide Nakamura, Tristan Biard, Miguel M. Sandin","doi":"10.1111/1462-2920.70098","DOIUrl":"https://doi.org/10.1111/1462-2920.70098","url":null,"abstract":"Radiolaria are heterotrophic protists abundant in the world's oceans, playing important roles in biogeochemical cycles. Some host photosynthetic algae, contributing to primary production. Such mixotrophic behaviour is believed to explain their success in oligotrophic waters, notably Collodaria, exclusively mixotrophic radiolarians within a gelatinous matrix. Yet, understanding of Radiolaria ecology is limited to direct observations, as they have so far withstood reproduction in culture and lack genome data. Sampling oligotrophic California Current revealed abundant, rarely observed Nassellaria of the genus Phlebarachnium, characterised to live within a gelatinous matrix. Phylogenetic reconstruction of ribosomal DNA suggests that distantly related Nassellaria lineages independently developed the ability to produce a gelatinous matrix ~150 million years ago. By matching physical samples with genetic data, we identified these rarely observed organisms in global datasets, revealing their affinity for oligotrophic conditions. Co-occurrence networks showed distinct biogeography patterns for gelatinous matrix-forming Radiolaria compared to those without. Results suggest the matrix might be an adaptation to oligotrophic waters, increasing the effective volume, favouring prey capture, and creating a larger microenvironment for symbionts, thus promoting ecological success in nutrient-depleted waters. This study advances our understanding of the adaptation of poorly known eukaryotic groups, specifically when evolution occurs independently across lineages.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 5","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70098","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143897146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-Throughput Tn-Seq Screens Identify Both Known and Novel Pseudomonas putida KT2440 Genes Involved in Metal Tolerance 高通量n- seq筛选鉴定已知和新的恶臭假单胞菌KT2440基因参与金属耐受性

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-04-29 DOI: 10.1111/1462-2920.70095

Kevin Royet, Laura Kergoat, Stefanie Lutz, Charlotte Oriol, Nicolas Parisot, Christian Schori, Christian H. Ahrens, Agnes Rodrigue, Erwan Gueguen

{"title":"High-Throughput Tn-Seq Screens Identify Both Known and Novel Pseudomonas putida KT2440 Genes Involved in Metal Tolerance","authors":"Kevin Royet, Laura Kergoat, Stefanie Lutz, Charlotte Oriol, Nicolas Parisot, Christian Schori, Christian H. Ahrens, Agnes Rodrigue, Erwan Gueguen","doi":"10.1111/1462-2920.70095","DOIUrl":"https://doi.org/10.1111/1462-2920.70095","url":null,"abstract":"Industrial and urban activities release toxic chemical waste into the environment. Pseudomonas putida, a soil bacterium, is known to degrade hydrocarbons and xenobiotics, and possesses numerous genes associated with heavy metal tolerance. Most studies on metal tolerance in P. putida focus solely on over- or underexpressed genes, potentially overlooking important genes with unchanged expression. This study employed a Tn-seq approach to identify the essential genes required for P. putida growth under metal stress. This method enables the identification of mutants with altered fitness in the presence of excess metals. The screen successfully identified a number of known genes implicated in metal resistance, including czcA-1, cadA-3, cadR, and pcoA2, thereby validating the approach. Further analyses using targeted mutagenesis and complementation assays revealed PP_5337 as a putative transcriptional regulator involved in copper tolerance and the two-component system RoxSR (PP_0887/PP_0888) as a key determinant of cadmium tolerance. Additionally, PP_1663 and PP_5002 were identified as contributing to cadmium and cobalt tolerance, respectively. This study provides the first evidence linking these genes to metal tolerance, highlighting gaps in our understanding of metal tolerance mechanisms in P. putida and demonstrating the utility of Tn-seq for identifying novel tolerance determinants.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 5","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70095","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143889165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Paradox of the Sub-Plankton: Plausible Mechanisms and Open Problems Underlying Strain-Level Diversity in Microbial Communities 浮游亚生物的悖论：微生物群落中菌株水平多样性的合理机制和开放问题

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-04-23 DOI: 10.1111/1462-2920.70094

Akshit Goyal, Griffin Chure

{"title":"Paradox of the Sub-Plankton: Plausible Mechanisms and Open Problems Underlying Strain-Level Diversity in Microbial Communities","authors":"Akshit Goyal, Griffin Chure","doi":"10.1111/1462-2920.70094","DOIUrl":"https://doi.org/10.1111/1462-2920.70094","url":null,"abstract":"Microbial communities are often complex and highly diverse, typically with dozens of species sharing spatially-restricted environments. Within these species, genetic and ecological variation often exists at a much finer scale, with closely related strains coexisting and competing. While the coexistence of strains in communities has been heavily explored over the past two decades, we have no self-consistent theory of how this diversity is maintained. This question challenges our conventional understanding of ecological coexistence, typically framed around species with clear phenotypic and ecological differences. In this review, we synthesise plausible mechanisms underlying strain-level diversity (termed microdiversity), focusing on niche-based mechanisms such as nutrient competition, neutral mechanisms such as migration, and evolutionary mechanisms such as horizontal gene transfer. We critically assess the strengths and caveats of these mechanisms, acknowledging key gaps that persist in linking genetic similarity to ecological divergence. Finally, we highlight how the origin and maintenance of microdiversity could pose a major challenge to conventional ecological thinking. We articulate a call-to-arms for a dialogue between well-designed experiments and new theoretical frameworks to address this grand conceptual challenge in understanding microbial biodiversity.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 4","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70094","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143865709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic Segregation and Functional Gene Clusters in Anaerobic Digestion Consortia 厌氧消化菌群的代谢分离和功能基因簇

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-04-22 DOI: 10.1111/1462-2920.70091

Yubo Wang, Ruoqun Zhang, Chunxiao Wang, Weifu Yan, Tong Zhang, Feng Ju

{"title":"Metabolic Segregation and Functional Gene Clusters in Anaerobic Digestion Consortia","authors":"Yubo Wang, Ruoqun Zhang, Chunxiao Wang, Weifu Yan, Tong Zhang, Feng Ju","doi":"10.1111/1462-2920.70091","DOIUrl":"https://doi.org/10.1111/1462-2920.70091","url":null,"abstract":"<div>\u0000 \u0000 A combined enrichment experiment and genome-centric meta-omics analysis demonstrated that metabolic specificity, rather than flexibility, governs the anaerobic digestion (AD) ecosystem. This study provides new insights into interspecies electron transfer in the AD process, highlighting a segregation in the metabolism of H2 and formate. Our findings show that H2 acts as the primary electron sink for recycling redox cofactors, including NAD+ and oxidised ferredoxin (Fdox), during primary fermentation, while formate is the dominant electron carrier in secondary fermentation, especially under conditions with elevated H2 concentrations. Importantly, no evidence of biochemical interconversion between H2 and formate was identified in the primary fermenting bacteria or in syntrophs enriched in this study. This segregation of H2 and formate metabolism likely benefits the anaerobic oxidation of butyrate and propionate with a higher tolerance to H2 accumulation. Moreover, this study highlights the functional partitioning among microbial populations in key AD niches: primary fermentation, secondary fermentation (syntrophic acetogenesis), hydrogenotrophic methanogenesis, and acetoclastic methanogenesis. Genome-centric analysis of the AD microbiome identified several key functional gene clusters, which could enhance genome-centric genotype–phenotype correlations, particularly for strict anaerobes that are difficult to isolate and characterise in pure culture.\u0000 </div>","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 4","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143861512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Warming is Associated With More Encoded Antimicrobial Resistance Genes and Transcriptions Within Five Drug Classes in Soil Bacteria: A Case Study and Synthesis 变暖与土壤细菌中5种药物类别中更多编码的抗微生物药物抗性基因和转录相关：一个案例研究和综合

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-04-22 DOI: 10.1111/1462-2920.70097

Melanie T. Hacopian, Alberto Barrón-Sandoval, Adriana L. Romero-Olivares, Renaud Berlemont, Kathleen K. Treseder

{"title":"Warming is Associated With More Encoded Antimicrobial Resistance Genes and Transcriptions Within Five Drug Classes in Soil Bacteria: A Case Study and Synthesis","authors":"Melanie T. Hacopian, Alberto Barrón-Sandoval, Adriana L. Romero-Olivares, Renaud Berlemont, Kathleen K. Treseder","doi":"10.1111/1462-2920.70097","DOIUrl":"https://doi.org/10.1111/1462-2920.70097","url":null,"abstract":"The effect of warming on anti-microbial resistance (AMR) genes in the environment has critical implications for public health but is little studied. We collected published soil bacterial genomes from the BV-BRC database and tested the correlation between reported optimal growth temperature and the number of encoded AMR genes. Furthermore, we tested the relationship between temperature and AMR gene transcription in a natural ecosystem by analysing soil transcriptomes from a warming manipulation experiment in an Alaskan boreal forest. We hypothesised that there is a positive relationship between warming and AMR prevalence in gene content in bacterial genomes and transcriptomic sequences, and that this effect would vary by drug class. Regarding the bacterial genomes, we found a positive relationship between the fraction of encoded AMR genes and the reported optimal temperature of soil bacteria. The drug classes tetracycline and lincosamide/macrolide/streptogramin had the strongest positive relationship with reported optimal temperature. For the case study in a natural ecosystem, we found 61 significantly upregulated AMR gene-associated transcripts spanning eight drug classes in warmed plots. In the Alaskan soil samples, we found that warming elicited the strongest positive effect on transcripts targeting lincosamide/streptogramin, beta-lactam and phenicol/quinolone antibiotics. Overall, higher temperatures were linked to AMR gene prevalence.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 4","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70097","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143861513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ZfpA-Dependent Quorum Sensing Shifts in Morphology and Secondary Metabolism in Aspergillus flavus zfpa依赖性群体感应在黄曲霉形态和次生代谢中的变化

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-04-22 DOI: 10.1111/1462-2920.70100

Benjamin Otoo, Dante G. Calise, Sung Chul Park, Jin Woo Bok, Nancy P. Keller, Mira Syahfriena Amir Rawa

{"title":"ZfpA-Dependent Quorum Sensing Shifts in Morphology and Secondary Metabolism in Aspergillus flavus","authors":"Benjamin Otoo, Dante G. Calise, Sung Chul Park, Jin Woo Bok, Nancy P. Keller, Mira Syahfriena Amir Rawa","doi":"10.1111/1462-2920.70100","DOIUrl":"https://doi.org/10.1111/1462-2920.70100","url":null,"abstract":"Development of the fungal pathogen Aspergillus flavus involves the balance of asexual spores (conidia) and overwintering hardened hyphal masses (sclerotia). This balance is achieved by an oxylipin-based density-dependent mechanism regulating the switch from sclerotia to conidia as population density increases in A. flavus. Here, we show the transcription factor ZfpA, required for normal oxylipin synthesis, regulates the morphology switch. ZfpA overexpression (OE::zfpA) accelerates the shift leading to increased conidial production and reduced sclerotial production under conditions normally supporting sclerotia formation. In contrast, zfpA deletion (ΔzfpA) produces more sclerotia than wild-type control. These morphology changes are coupled with changes in tissue-specific secondary metabolites. Specifically, the production of four sclerotial metabolites (oxyasparasone A, hydroxyaflatrem, aflavinine, and kotanin) decreases in OE::zfpA whereas the hyphal metabolite aspergillic acid is upregulated in this mutant. Chemical profiling of OE::zfpA compared to a double mutant where the aspergillic acid non-ribosomal synthetase was deleted in the OE::zfpA background confirmed synthesis of known aspergillic acid pathway products as well as putative Val-derived pyrazinones involved in metal chelation. These findings offer valuable insights into the quorum sensing networks connecting fungal development and tissue-specific secondary metabolite production.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 4","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70100","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143861635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proteomic Analysis of Marine Bacteriophages: Structural Conservation, Post-Translational Modifications, and Phage–Host Interactions 海洋噬菌体的蛋白质组学分析：结构保护、翻译后修饰和噬菌体-宿主相互作用

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-04-22 DOI: 10.1111/1462-2920.70099

Shuzhen Wei, Anan Wang, Lanlan Cai, Ruijie Ma, Longfei Lu, Jiangtao Li, Rui Zhang

{"title":"Proteomic Analysis of Marine Bacteriophages: Structural Conservation, Post-Translational Modifications, and Phage–Host Interactions","authors":"Shuzhen Wei, Anan Wang, Lanlan Cai, Ruijie Ma, Longfei Lu, Jiangtao Li, Rui Zhang","doi":"10.1111/1462-2920.70099","DOIUrl":"https://doi.org/10.1111/1462-2920.70099","url":null,"abstract":"Marine bacteriophages, the most abundant biological entities in marine ecosystems, are essential in biogeochemical cycling. Despite extensive genomic data, many phage genes remain uncharacterised, creating a gap between genomic diversity and gene function knowledge. This gap limits our understanding of phage life cycles, assembly, and host interactions. In this study, we used mass spectrometry to profile the proteomes of 13 marine phages from diverse lifestyles and hosts. The analysis accurately annotated hypothetical genes, mapped virion protein arrangements, and revealed structural similarities among phages infecting the same host, particularly in tail fibre proteins. Protein structure comparisons showed conservation and variability in head and tail proteins, particularly in key domains involved in virion stabilisation and host recognition. For the first time, we identified post-translational modifications (PTMs) in marine phage proteins, which may enhance phage adaptability and help evade host immune systems. These findings suggest that phages optimise their infection strategies through structural variations and PTM modifications, improving their adaptability and host interactions.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 4","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70099","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143861634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0