Environmental microbiology最新文献

Adaptive Responses of Cyanobacteria to Phosphate Limitation: A Focus on Marine Diazotrophs

IF 5.1 2区生物学

Environmental microbiology Pub Date : 2024-12-23 DOI: 10.1111/1462-2920.70023

Chloé Caille, Solange Duhamel, Amel Latifi, Sophie Rabouille

{"title":"Adaptive Responses of Cyanobacteria to Phosphate Limitation: A Focus on Marine Diazotrophs","authors":"Chloé Caille, Solange Duhamel, Amel Latifi, Sophie Rabouille","doi":"10.1111/1462-2920.70023","DOIUrl":"https://doi.org/10.1111/1462-2920.70023","url":null,"abstract":"Phosphorus is an essential component of numerous macromolecules and is vital for life. Its availability significantly influences primary production, particularly in oligotrophic environments. Marine diazotrophic cyanobacteria, which play key roles in biogeochemical cycles through nitrogen fixation (N2 fixation), have adapted to thrive in phosphate (Pi)-poor areas. However, the molecular mechanisms that facilitate their adaptation to such conditions remain incompletely understood. Bacteria have evolved various strategies to cope with Pi limitation, including detecting Pi availability, utilising high-affinity Pi transporters, and hydrolyzing dissolved organic phosphorus (DOP) with various enzymes. This review synthesises current knowledge regarding how cyanobacteria adapt to Pi scarcity, with particular emphasis on subtropical marine free-living diazotrophs and their ability to utilise diverse DOP molecules. Omics approaches, such as (meta)genomics and (meta)transcriptomics, reveal the resilience of marine diazotrophs in the face of Pi scarcity and highlight the need for further research into their molecular adaptive strategies. Adaptation to Pi limitation is often intertwined with the broader response of cyanobacteria to multiple limitations and stresses. This underscores the importance of understanding Pi adaptation to assess the ecological resilience of these crucial microorganisms in dynamic environments, particularly in the context of global climate change.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"24 1","pages":""},"PeriodicalIF":5.1,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142874434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial Community of a Sandy Beach Subterranean Estuary is Spatially Heterogeneous and Impacted by Winter Waves

IF 5.1 2区生物学

Environmental microbiology Pub Date : 2024-12-22 DOI: 10.1111/1462-2920.70009

Jessica A. Bullington, Kathryn Langenfeld, Jacob R. Phaneuf, Alexandria B. Boehm, Christopher A. Francis

{"title":"Microbial Community of a Sandy Beach Subterranean Estuary is Spatially Heterogeneous and Impacted by Winter Waves","authors":"Jessica A. Bullington, Kathryn Langenfeld, Jacob R. Phaneuf, Alexandria B. Boehm, Christopher A. Francis","doi":"10.1111/1462-2920.70009","DOIUrl":"https://doi.org/10.1111/1462-2920.70009","url":null,"abstract":"Subterranean estuaries (STEs) are critical ecosystems at the interface of meteoric groundwater and subsurface seawater that are threatened by sea level rise. To characterize the influence of tides and waves on the STE microbial community, we collected porewater samples from a high-energy beach STE at Stinson Beach, California, USA, over the two-week neap-spring tidal transition during both a wet and dry season. The microbial community, analyzed by 16S rRNA gene (V4) amplicon sequencing, clustered according to consistent physicochemical features found within STEs. The porewater community harbored relatively abundant Proteobacteria, Verrucomicrobiota, and Bacteroidota, as well as members of the archaeal DPANN superphylum and bacterial Candidate Phyla Radiation (CPR). Tidal conditions were not associated with microbial community composition; however, a wave overtopping event significantly impacted the beach microbiome. As a baseline for environmental change, our results elucidate the unique dynamics of a STE microbiome with unprecedented temporal resolution, highlighting the transport of cellular material through beach porewater due to waves.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"32 1","pages":""},"PeriodicalIF":5.1,"publicationDate":"2024-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142874433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unravelling a Latent Pathobiome Across Coral Reef Biotopes

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2024-12-20 DOI: 10.1111/1462-2920.70008

Vanessa Oliveira, Daniel F. R. Cleary, Ana R. M. Polónia, Yusheng M. Huang, Ulisses Rocha, Nicole J. de Voogd, Newton C. M. Gomes

{"title":"Unravelling a Latent Pathobiome Across Coral Reef Biotopes","authors":"Vanessa Oliveira, Daniel F. R. Cleary, Ana R. M. Polónia, Yusheng M. Huang, Ulisses Rocha, Nicole J. de Voogd, Newton C. M. Gomes","doi":"10.1111/1462-2920.70008","DOIUrl":"10.1111/1462-2920.70008","url":null,"abstract":"<div>\u0000 \u0000 Previous studies on disease in coral reef organisms have neglected the natural distribution of potential pathogens and the genetic factors that underlie disease incidence. This study explores the intricate associations between hosts, microbial communities, putative pathogens, antibiotic resistance genes (ARGs) and virulence factors (VFs) across diverse coral reef biotopes. We observed a substantial compositional overlap of putative bacterial pathogens, VFs and ARGs across biotopes, consistent with the ‘everything is everywhere, but the environment selects’ hypothesis. However, flatworms and soft corals deviated from this pattern, harbouring the least diverse microbial communities and the lowest diversity of putative pathogens and ARGs. Notably, our study revealed a significant congruence between the distribution of putative pathogens, ARGs and microbial assemblages across different biotopes, suggesting an association between pathogen and ARG occurrence. This study sheds light on the existence of this latent pathobiome, the disturbance of which may contribute to disease onset in coral reef organisms.\u0000 </div>","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"26 12","pages":""},"PeriodicalIF":4.3,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142867063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selective Pressure Influences Inter-Biome Dispersal in the Assembly of Saline Microbial Communities

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2024-12-19 DOI: 10.1111/1462-2920.70019

Mateu Menéndez-Serra, Joan Cáliz, Xavier Triadó-Margarit, David Alonso, Emilio O. Casamayor

{"title":"Selective Pressure Influences Inter-Biome Dispersal in the Assembly of Saline Microbial Communities","authors":"Mateu Menéndez-Serra, Joan Cáliz, Xavier Triadó-Margarit, David Alonso, Emilio O. Casamayor","doi":"10.1111/1462-2920.70019","DOIUrl":"10.1111/1462-2920.70019","url":null,"abstract":"Selection and dispersal are the primary processes influencing community assembly at both global and regional scales. Although the effectiveness of dispersal is often examined within the same biome, microscopic organisms demonstrate the capability to colonise and thrive across different biomes. In this study, we evaluated the relationship between (i) aquatic, (ii) sedimentary and (iii) aerial microbial communities, and how local selective pressures influence the potential impact of inter-biome dispersal, focusing on the salinity gradient stress over time in ephemeral saline lakes. Our taxonomic ordination analyses revealed that the three communities were distinctly segregated yet interconnected by shared populations. Organisms prevalent across the three biomes exhibited cosmopolitan behaviour based on global databases, indicating an inherent ability to cross biome boundaries. Cosmopolitan groups dominated the planktonic community at lower salinities but gradually diminished as salinity increased, resulting in communities dominated by aquatic specialists with more restricted environmental distributions. The aerial community was primarily composed of generalists, although airborne halophiles were also identified, suggesting long-range dispersal as a source of colonisers in isolated extremophile environments. Our findings contribute to a better understanding of the dynamic interplay between dispersal and selective pressures on community assembly across biomes, highlighting the significance of aerial microbiota in remote colonisation.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"26 12","pages":""},"PeriodicalIF":4.3,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70019","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142857718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oxylipin Receptors and Their Role in Inter-Partner Signalling in a Model Cnidarian-Dinoflagellate Symbiosis

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2024-12-19 DOI: 10.1111/1462-2920.70015

Andrea G. Gamba, Clinton A. Oakley, Immy A. Ashley, Arthur R. Grossman, Virginia M. Weis, David J. Suggett, Simon K. Davy

{"title":"Oxylipin Receptors and Their Role in Inter-Partner Signalling in a Model Cnidarian-Dinoflagellate Symbiosis","authors":"Andrea G. Gamba, Clinton A. Oakley, Immy A. Ashley, Arthur R. Grossman, Virginia M. Weis, David J. Suggett, Simon K. Davy","doi":"10.1111/1462-2920.70015","DOIUrl":"10.1111/1462-2920.70015","url":null,"abstract":"<div>\u0000 \u0000 Oxylipin signalling is central in biology, mediating processes such as cellular homeostasis, inflammation and molecular signalling. It may also facilitate inter-partner communication in the cnidarian-dinoflagellate symbiosis, though this aspect remains understudied. In this study, four oxylipin receptors were characterised using immunohistochemistry and immunoblotting in the sea anemone Exaiptasia diaphana (‘Aiptasia’): Prostaglandin E2 receptor 2 (EP2) and 4 (EP4), Transient Receptor Potential cation channel A1 (TRPA1) and Glutamate Receptor Ionotropic, Kainate 2 (GRIK2). Receptor abundance and localisation were compared between aposymbiotic anemones and symbiotic anemones hosting either native Breviolum minutum or non-native Durusdinium trenchii. All receptors were localised to the putative symbiosome of freshly isolated symbionts, suggesting a role in host-symbiont crosstalk. EP2, EP4 and TRPA1 abundance decreased in the gastrodermis of anemones hosting B. minutum, indicating potential downregulation of pathways mediated by these receptors. In contrast, GRIK2 abundance increased in anemones hosting D. trenchii in both the epidermis and gastrodermis; GRIK2 acts as a chemosensor of potential pathogens in other systems and could play a similar role here given D. trenchii's reputation as a sub-optimal partner for Aiptasia. This study contributes to the understanding of oxylipin signalling in the cnidarian-dinoflagellate symbiosis and supports further exploration of host-symbiont molecular signalling.\u0000 </div>","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"26 12","pages":""},"PeriodicalIF":4.3,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142857717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A CoA-Transferase and Acyl-CoA Dehydrogenase Convert 2-(Carboxymethyl)cyclohexane-1-Carboxyl-CoA During Anaerobic Naphthalene Degradation

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2024-12-19 DOI: 10.1111/1462-2920.70013

Yachao Kong, Jan Riebe, Malte Feßner, Torsten Schaller, Christoph Wölper, Florian Stappert, Sven W. Meckelmann, Matthias Krajnc, Philip Weyrauch, Oliver J. Schmitz, Christian Merten, Jochen Niemeyer, Xiaoke Hu, Rainer U. Meckenstock

{"title":"A CoA-Transferase and Acyl-CoA Dehydrogenase Convert 2-(Carboxymethyl)cyclohexane-1-Carboxyl-CoA During Anaerobic Naphthalene Degradation","authors":"Yachao Kong, Jan Riebe, Malte Feßner, Torsten Schaller, Christoph Wölper, Florian Stappert, Sven W. Meckelmann, Matthias Krajnc, Philip Weyrauch, Oliver J. Schmitz, Christian Merten, Jochen Niemeyer, Xiaoke Hu, Rainer U. Meckenstock","doi":"10.1111/1462-2920.70013","DOIUrl":"10.1111/1462-2920.70013","url":null,"abstract":"The CoA thioester of 2-(carboxymethyl)cyclohexane-1-carboxylic acid has been identified as a metabolite in anaerobic naphthalene degradation by the sulfate-reducing culture N47. This study identified and characterised two acyl-CoA dehydrogenases (ThnO/ThnT) and an intramolecular CoA-transferase (ThnP) encoded within the substrate-induced thn operon, which contains genes for anaerobic degradation of naphthalene. ThnP is a CoA transferase belonging to the family I (Cat 1 subgroup) that catalyses the intramolecular CoA transfer from the carboxyl group of 2-(carboxymethyl)cyclohexane-1-carboxyl-CoA to its carboxymethyl moiety, forming 2-carboxycyclohexylacetyl-CoA. Neither acetyl-CoA nor succinyl-CoA functions as an exogenous CoA donor for this reaction. The flavin-dependent homotetrameric dehydrogenase ThnO is specific for (1R,2R)-2-carboxycyclohexylacetyl-CoA with an apparent Km value of 61.5 μM, whereas ThnT is a promiscuous enzyme catalysing the same reaction at lower rates. Identifying these three enzymes confirmed the involvement of the thn gene cluster in the anaerobic naphthalene degradation pathway. This study establishes a modified metabolic pathway for anaerobic naphthalene degradation upstream of 2-(carboxymethyl)cyclohexane-1-carboxyl-CoA and provides further insight into the subsequent second-ring cleavage reaction.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"26 12","pages":""},"PeriodicalIF":4.3,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70013","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142857719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimal Cell Length for Exploration and Exploitation in Chemotactic Planktonic Bacteria

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2024-12-19 DOI: 10.1111/1462-2920.70021

Òscar Guadayol, Rudi Schuech, Stuart Humphries

{"title":"Optimal Cell Length for Exploration and Exploitation in Chemotactic Planktonic Bacteria","authors":"Òscar Guadayol, Rudi Schuech, Stuart Humphries","doi":"10.1111/1462-2920.70021","DOIUrl":"10.1111/1462-2920.70021","url":null,"abstract":"Elongated morphologies are prevalent among motile bacterioplankton in aquatic systems. This is often attributed to enhanced chemotactic ability, but how long is best? We hypothesized the existence of an optimal cell length for efficient chemotaxis resulting from shape-imposed physical constraints acting on the trade-off between rapid exploration versus efficient exploitation of nutrient sources. To test this hypothesis, we evaluated the chemotactic performance of elongated cephalexin-treated Escherichia coli towards α-methyl-aspartate in a microfluidic device creating linear, stable and quiescent chemical gradients. Our experiments showed cells of intermediate length aggregating most tightly to the chemoattractant source. A sensitivity analysis of an Individual-Based-Model replicating these results showed that 1) cells of intermediate length are optimal at transient states, whereas at steady state longest cells are best, 2) poor chemotactic performance of very short cells is caused by directionality loss, and 3) long cells are penalized by brief, slow runs. Finally, we evaluated chemotactic performance of cells of different length with simulations of a phycosphere, and found that long cells swimming in a run-and-reverse pattern with extended runs and moderate speeds are most efficient in this microenvironment. Overall, our results suggest that the stability of the chemical landscape plays a role in cell-size selection.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"26 12","pages":""},"PeriodicalIF":4.3,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70021","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142857715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New Campylobacter Lineages in New Zealand Freshwater: Pathogenesis and Public Health Implications

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2024-12-16 DOI: 10.1111/1462-2920.70016

Adrian L. Cookson, Sara Burgess, Anne C. Midwinter, Jonathan C. Marshall, Marie Moinet, Lynn Rogers, Ahmed Fayaz, Patrick J. Biggs, Gale Brightwell

{"title":"New Campylobacter Lineages in New Zealand Freshwater: Pathogenesis and Public Health Implications","authors":"Adrian L. Cookson, Sara Burgess, Anne C. Midwinter, Jonathan C. Marshall, Marie Moinet, Lynn Rogers, Ahmed Fayaz, Patrick J. Biggs, Gale Brightwell","doi":"10.1111/1462-2920.70016","DOIUrl":"10.1111/1462-2920.70016","url":null,"abstract":"This study investigated the diversity of thermophilic Campylobacter species isolated from three New Zealand freshwater catchments affected by pastoral and urban activities. Utilising matrix-assisted laser desorption ionisation-time of flight and whole genome sequence analysis, the study identified Campylobacter jejuni (n = 46, 46.0%), C. coli (n = 39, 39%), C. lari (n = 4, 4.0%), and two novel Campylobacter species lineages (n = 11, 11%). Core genome sequence analysis provided evidence of prolonged persistence or continuous faecal shedding of closely related strains. The C. jejuni isolates displayed distinct sequence types (STs) associated with human, ruminant, and environmental sources, whereas the C. coli STs included waterborne ST3302 and ST7774. Recombination events affecting loci implicated in human pathogenesis and environmental persistence were observed, particularly in the cdtABC operon (encoding the cytolethal distending toxin) of non-human C. jejuni STs. A low diversity of antimicrobial resistance genes (aadE-Cc in C. coli), with genotype/phenotype concordance for tetracycline resistance (tetO) in three ST177 isolates, was noted. The data suggest the existence of two types of naturalised waterborne Campylobacter: environmentally persistent strains originating from waterbirds and new environmental species not linked to human campylobacteriosis. Identifying and understanding naturalised Campylobacter species is crucial for accurate waterborne public health risk assessments and the effective allocation of resources for water quality management.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"26 12","pages":""},"PeriodicalIF":4.3,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70016","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142832158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pavlovian-Type Learning in Environmental Bacteria: Regulation of Herbicide Resistance by Arsenic in Pseudomonas putida

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2024-12-12 DOI: 10.1111/1462-2920.70012

David Paez-Espino, Gonzalo Durante-Rodríguez, Elena Alonso Fernandes, Manuel Carmona, Victor de Lorenzo

{"title":"Pavlovian-Type Learning in Environmental Bacteria: Regulation of Herbicide Resistance by Arsenic in Pseudomonas putida","authors":"David Paez-Espino, Gonzalo Durante-Rodríguez, Elena Alonso Fernandes, Manuel Carmona, Victor de Lorenzo","doi":"10.1111/1462-2920.70012","DOIUrl":"10.1111/1462-2920.70012","url":null,"abstract":"The canonical arsRBC genes of the ars1 operon in Pseudomonas putida KT2440, which confer tolerance to arsenate and arsenite, are followed by a series of additional ORFs culminating in phoN1. The phoN1 gene encodes an acetyltransferase that imparts resistance to the glutamine synthetase inhibitor herbicide phosphinothricin (PPT). The co-expression of phoN1 and ars genes in response to environmental arsenic, along with the physiological effects, was analysed through transcriptomics of cells exposed to the oxyanion and phenotypic characterization of P. putida strains deficient in different components of the bifan motif governing arsenic resistance in this bacterium. Genetic separation of arsRBC and phoN1 revealed that their associated phenotypes operate independently, indicating that their natural co-regulation is not functionally required for simultaneous response to the same signal. The data suggest a scenario of associative evolution, akin to Pavlovian conditioning, where two unrelated but frequently co-occurring signals result in one regulating the other's response – even if there is no functional link between the signal and the response. Such surrogate regulatory events may provide an efficient solution to complex regulatory challenges and serve as a genetic patch to address transient gaps in evolving regulatory networks.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"26 12","pages":""},"PeriodicalIF":4.3,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70012","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142815714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gene Expression by a Model Fungus in the Ascomycota Provides Insight Into the Decay of Fungal Necromass

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2024-12-08 DOI: 10.1111/1462-2920.70006

Irshad Ul Haq, Peter Kennedy, Kathryn M. Schreiner, Julia C. Agnich, Jonathan S. Schilling

{"title":"Gene Expression by a Model Fungus in the Ascomycota Provides Insight Into the Decay of Fungal Necromass","authors":"Irshad Ul Haq, Peter Kennedy, Kathryn M. Schreiner, Julia C. Agnich, Jonathan S. Schilling","doi":"10.1111/1462-2920.70006","DOIUrl":"10.1111/1462-2920.70006","url":null,"abstract":"Dead fungal cells, known as necromass, are increasingly recognised as significant contributors to long-term soil carbon pools, yet the genes involved in necromass decomposition are poorly understood. In particular, how microorganisms degrade necromass with differing initial cell wall chemical compositions using carbohydrate-active enzymes (CAZymes) has not been well studied. Based on the frequent occurrence and high abundance of the fungal genus Trichoderma on decaying fungal necromass in situ, we grew Trichoderma reesei RUT-C30 on low and high melanin necromass of Hyaloscypha bicolor (Ascomycota) in liquid cultures and assessed T. reesei gene expression relative to each other and relative to glucose. Transcriptome data revealed that T. reesei up-regulated many genes (over 100; necromass versus glucose substrate) coding for CAZymes, including enzymes that would target individual layers of an Ascomycota fungal cell wall. We also observed differential expression of protease- and laccase-encoding genes on high versus low melanin necromass, highlighting a subset of genes (fewer than 15) possibly linked to the deconstruction of melanin, a cell wall constituent that limits necromass decay rates in nature. Collectively, these results advance our understanding of the genomic traits underpinning the rates and fates of carbon turnover in an understudied pool of Earth's belowground carbon, fungal necromass.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"26 12","pages":""},"PeriodicalIF":4.3,"publicationDate":"2024-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70006","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142793544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0