Environmental microbiology最新文献_第2页

Distinct Patterns of Antibiotic Sensitivities in Ammonia-Oxidising Archaea

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-03-11 DOI: 10.1111/1462-2920.70063

Timothy Klein, Logan H. Hodgskiss, Max Dreer, J. Colin Murrell, Matthew I. Hutchings, Christa Schleper, Laura E. Lehtovirta-Morley

{"title":"Distinct Patterns of Antibiotic Sensitivities in Ammonia-Oxidising Archaea","authors":"Timothy Klein, Logan H. Hodgskiss, Max Dreer, J. Colin Murrell, Matthew I. Hutchings, Christa Schleper, Laura E. Lehtovirta-Morley","doi":"10.1111/1462-2920.70063","DOIUrl":"https://doi.org/10.1111/1462-2920.70063","url":null,"abstract":"Ammonia-oxidising archaea (AOA) are important microorganisms contributing towards the nitrogen flux in the environment. Unlike archaea from other major phyla, genetic tools are yet to be developed for the AOA, and identification of antibiotic resistance markers for selecting mutants is required for a genetic system. The aim of this study was to test the effects of selected antibiotics (hygromycin B, neomycin, apramycin, puromycin, novobiocin) on pure cultures of three well studied AOA strains, ‘Candidatus Nitrosocosmicus franklandianus C13’, Nitrososphaera viennensis EN76 and Nitrosopumilus maritimus SCM1. Puromycin, hygromycin B and neomycin inhibited some but not all tested archaeal strains. All strains were resistant to apramycin and inhibited by novobiocin to various degrees. As N. viennensis EN76 was relatively more resistant to the tested antibiotics, a wider range of concentrations and compounds (chloramphenicol, trimethoprim, statins) was tested against this strain. N. viennensis EN76 was inhibited by trimethoprim, but not by chloramphenicol, and growth recovered within days in the presence of simvastatin, suggesting either degradation of, or spontaneous resistance against, this compound. This study highlights the physiological differences between different genera of AOA and has identified new candidate antibiotics for selective enrichment and the development of selectable markers for genetic systems in AOA.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 3","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70063","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143595237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the Exclusive Isolation of Pseudomonas syringae in Peltigera Lichens via Metabolite Analysis and Growth Assays

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-03-10 DOI: 10.1111/1462-2920.70057

Natalia Ramírez, Diana Vinchira-Villarraga, Mojgan Rabiey, Margrét Auður Sigurbjörnsdóttir, Starri Heidmarsson, Oddur Vilhelmsson, Robert W. Jackson

{"title":"Exploring the Exclusive Isolation of Pseudomonas syringae in Peltigera Lichens via Metabolite Analysis and Growth Assays","authors":"Natalia Ramírez, Diana Vinchira-Villarraga, Mojgan Rabiey, Margrét Auður Sigurbjörnsdóttir, Starri Heidmarsson, Oddur Vilhelmsson, Robert W. Jackson","doi":"10.1111/1462-2920.70057","DOIUrl":"https://doi.org/10.1111/1462-2920.70057","url":null,"abstract":"<div>\u0000 \u0000 The specific association of the potentially plant-pathogenic Pseudomonas syringae with Peltigera lichens raises questions about the factors driving this host specificity. To explore this, the metabolic profile of seven lichen species belonging to three genera (Cladonia, Peltigera and Stereocaulon) was analysed using LC-MSMS. In addition, we assessed the growth of P. syringae strains in media supplemented with extracts from each lichen species. This revealed that Peltigera exhibits lower metabolite richness compared to other genera, but shows a higher chemical investment in specific compounds. Growth kinetics showed comparable P. syringae growth across lichen-supplemented media, except for Cladonia arbuscula and Cladonia sp., where the former exhibited lower growth rates. Inhibition assays with lichen extracts showed no inhibition of P. syringae. The lichen metabolome is predominantly composed of lipids and organic acids. Furthermore, specific compounds, such as aminoglycosides, may facilitate P. syringae presence in Peltigera by inhibiting Bacillus subtilis and other antagonists. In addition, compounds absent in Peltigera, like anthracene, might serve as a carbon source inhibitor like Bacillus velezensis.\u0000 </div>","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 3","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143595150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New Insight Into the Mechanism of Nitrite Enhancement on Heterotrophic Nitrification and Aerobic Denitrification Bacterium in Gene Expression

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-03-10 DOI: 10.1111/1462-2920.70080

Chunxia Zheng, Xiongfeng Zhang, Tengxia He, Pan Wu, Wenruo Wu, Manman Zhang, Hong Zhao

{"title":"New Insight Into the Mechanism of Nitrite Enhancement on Heterotrophic Nitrification and Aerobic Denitrification Bacterium in Gene Expression","authors":"Chunxia Zheng, Xiongfeng Zhang, Tengxia He, Pan Wu, Wenruo Wu, Manman Zhang, Hong Zhao","doi":"10.1111/1462-2920.70080","DOIUrl":"https://doi.org/10.1111/1462-2920.70080","url":null,"abstract":"<div>\u0000 \u0000 The growth and nitrogen metabolism of heterotrophic nitrification-aerobic denitrification (HN-AD) bacteria are affected by nitrite, but the mechanisms underlying this for strain Acinetobacter johnsonii EN-J1 are unclear. In this study, the addition of 10 mg/L nitrite increased the reduction rate of ammonium by 1.0 mg/L/h, and 20 mg/L nitrite increased the reduction rate of nitrate by 3.9 mg/L/h. Compared with the control, the nitrate reductase activity, electron transfer activity, and adenosine triphosphate content of EN-J1 were enhanced by 142.0%, 278.0% and 279.0%, respectively, in the nitrate removal process after the addition of 20 mg/L nitrite. The whole genome was annotated with nitrogen removal genes such as narGHI, narK, nsrR, nirBD, nasA, glnA, gltB, gdhA and amt. Transcriptome analysis showed that nitrite triggered significant upregulation of several key pathways, including nitrogen metabolism, the tricarboxylic acid cycle, and amino acid metabolism for enhancing denitrification. The expression of key denitrification genes (narG, narK, hmp, nirBD, glnA and nasA) was detected by real-time quantitative polymerase chain reaction. These results suggested that nitrite enhances denitrification by increasing the expression of denitrification genes, electron transfer and adenosine triphosphate levels, which is important for elucidating the mechanism of nitrite promotion of biological nitrogen removal efficiency.\u0000 </div>","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 3","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143595148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Abundance, Identity, and Potential Diazotrophic Activity of nifH-Containing Organisms at Marine Cold Seeps

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-03-10 DOI: 10.1111/1462-2920.70058

Amanda C. Semler, Emily R. Paris, Mikaela Salvador, Anne E. Dekas

{"title":"Abundance, Identity, and Potential Diazotrophic Activity of nifH-Containing Organisms at Marine Cold Seeps","authors":"Amanda C. Semler, Emily R. Paris, Mikaela Salvador, Anne E. Dekas","doi":"10.1111/1462-2920.70058","DOIUrl":"https://doi.org/10.1111/1462-2920.70058","url":null,"abstract":"<div>\u0000 \u0000 Diazotrophic microorganisms alleviate nitrogen limitation at marine cold seeps using nitrogenase, encoded in part by the gene nifH. Here, we investigated nifH-containing organisms (NCOs) inside and outside six biogeochemically heterogeneous seeps using amplicon sequencing and quantitative real-time PCR (qPCR) of nifH genes and transcripts. We detected nifH genes affiliated with phylogenetically and metabolically diverse organisms spanning 18 bacterial and archaeal phyla (17 within seeps). Detected NCOs included methane-oxidising ANME-2 archaea and sulfate-reducing Desulfobacteraceae, which have been shown to fix nitrogen at seeps previously, as well as Desulfuromonadales and putatively hydrocarbon-oxidising Desulfoglaeba and Candidatus Methanoliparia. We detected nifH transcripts at five of the six seeps, suggesting widespread diazotrophic activity. We corrected our qPCR data based on our amplicon results, which found that 71% of recovered sequences were not bona fide nifH, and we recommend a similar correction in future qPCR studies that use broad nifH primers. NifH abundance was up to three orders of magnitude higher within seeps, was correlated with mcrA abundance, and, when corrected, was negatively correlated with porewater ammonium < 25 μM, consistent with the inhibition of diazotrophy by ammonium. Our findings expand the known diversity of NCOs at seeps and emphasise seeps as hotspots for deep-sea diazotrophy.\u0000 </div>","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 3","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143595149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantity Versus Quality: Links Between Soil Organic Matter and Bacterial Community Composition Along a Geoclimatic Gradient

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-03-08 DOI: 10.1111/1462-2920.70070

Daniel Wasner, Xingguo Han, Joerg Schnecker, Aline Frossard, Erick Zagal Venegas, Sebastian Doetterl

{"title":"Quantity Versus Quality: Links Between Soil Organic Matter and Bacterial Community Composition Along a Geoclimatic Gradient","authors":"Daniel Wasner, Xingguo Han, Joerg Schnecker, Aline Frossard, Erick Zagal Venegas, Sebastian Doetterl","doi":"10.1111/1462-2920.70070","DOIUrl":"https://doi.org/10.1111/1462-2920.70070","url":null,"abstract":"<div>\u0000 \u0000 Soil organic matter (SOM) quantity drives soil bacterial community composition from the regional to global scale. Qualitative characteristics of SOM are known to affect soil bacterial communities in manipulation experiments. However, it remains unresolved how strongly SOM characteristics affect soil bacterial community composition at the macroscale. Here, we investigated how quantity versus qualitative characteristics of SOM shape community composition along a biogeochemical gradient of grassland soils. We assessed relative abundance patterns of soil bacteria and characterised SOM based on scalable methods. Soils with higher SOM content (along a continuum between 0.6% and 18.7% SOC) and acidic pH (along a continuum between pH 4.1–6.7) hosted fewer narrowly distributed taxa (i.e., taxa occurring in few sites) and therefore had lower bacterial alpha diversity. We could explain a larger fraction of bacterial community composition (up to 59.6% of 16S rRNA reads) in these soils. Consequently, we understand community composition in low-SOM soils less than in high-SOM soils, because the drivers of narrowly distributed taxa remain poorly understood. Qualitative SOM characteristics did not strongly affect biogeographical patterns of widely distributed soil bacterial taxa. This suggests that broad aspects of SOM quality do not dominate soil bacterial community composition at the investigated macroscale.\u0000 </div>","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 3","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143571426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Influence of Symbiont Identity on the Proteomic and Metabolomic Responses of the Model Cnidarian Aiptasia to Thermal Stress

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-03-08 DOI: 10.1111/1462-2920.70073

Bobby Lust, Jennifer L. Matthews, Clinton A. Oakley, Robert E. Lewis, Himasha Mendis, Lifeng Peng, Arthur R. Grossman, Virginia M. Weis, Simon K. Davy

{"title":"The Influence of Symbiont Identity on the Proteomic and Metabolomic Responses of the Model Cnidarian Aiptasia to Thermal Stress","authors":"Bobby Lust, Jennifer L. Matthews, Clinton A. Oakley, Robert E. Lewis, Himasha Mendis, Lifeng Peng, Arthur R. Grossman, Virginia M. Weis, Simon K. Davy","doi":"10.1111/1462-2920.70073","DOIUrl":"https://doi.org/10.1111/1462-2920.70073","url":null,"abstract":"We examined the effects of symbiont identity and heat stress on the host metabolome and proteome in the cnidarian–dinoflagellate symbiosis. Exaiptasia diaphana (‘Aiptasia’) was inoculated with its homologous (i.e., native) symbiont Breviolum minutum or a heterologous (i.e., non-native) symbiont (Symbiodinium microadriaticum; Durusdinium trenchii) and thermally stressed. Integrated metabolome and proteome analyses characterised host thermal responses between symbioses, with clear evidence of enhanced nutritional deprivation and cellular stress in hosts harbouring heterologous symbionts following temperature stress. Host metabolomes were partially distinct at the control temperature; however, thermal stress caused metabolomes of anemones containing the two heterologous symbionts to become more alike and more distinct from those containing B. minutum. While these patterns could be partly explained by innate symbiont-specific differences, they may also reflect differences in symbiont density, as under control conditions D. trenchii attained 60% and S. microadriaticum 15% of the density attained by B. minutum, and at elevated temperature only D. trenchii–colonised anemones bleached (60% loss). Our findings add to a growing literature that highlights the physiological limits of partner switching as a means of adaptation to global warming. However, we also provide tentative evidence for improved metabolic functioning with a heterologous symbiont (D. trenchii) after sustained symbiosis.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 3","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70073","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143571425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Photoheterotroph Dokdonia sp. MED134 Modulates the Expression of Resource Acquisition and Anaplerotic Carbon Fixation Pathways in Response to Temperature

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-03-05 DOI: 10.1111/1462-2920.70071

José M. González, Semidán Robaina-Estévez, Ana María Cabello, Antonio S. Palacio, Ruairí Gallagher, Ángel López-Urrutia, Laura Alonso-Sáez

{"title":"The Photoheterotroph Dokdonia sp. MED134 Modulates the Expression of Resource Acquisition and Anaplerotic Carbon Fixation Pathways in Response to Temperature","authors":"José M. González, Semidán Robaina-Estévez, Ana María Cabello, Antonio S. Palacio, Ruairí Gallagher, Ángel López-Urrutia, Laura Alonso-Sáez","doi":"10.1111/1462-2920.70071","DOIUrl":"https://doi.org/10.1111/1462-2920.70071","url":null,"abstract":"<div>\u0000 \u0000 Temperature has an overriding impact on microbial physiology, but the molecular basis of thermal acclimation in many ecologically relevant marine bacterial taxa remains unexplored. We used quantitative transcriptomics to analyse the transcriptional reprogramming of a proteorhodopsin-based photoheterotroph, Dokdonia sp. MED134, during thermal acclimation from 10°C to 34°C. Temperature significantly impacted the expression of most MED134 genes (84%). Marker genes of the general stress response were induced towards cold temperature (10°C). Conversely, highly expressed genes associated with DNA replication and resource acquisition, like TonB-dependent transporters and gliding motility genes, were upregulated towards warm temperatures along this thermal range, when growth rates were fast. The mRNA transcript abundance of most genes related to the TCA cycle was not differentially expressed by temperature. By contrast, the expression of genes associated with anaplerotic carbon fixation was significantly enhanced at the optimum growth temperature (25°C). The expression of the proteorhodopsin was minimum at 10°C, and its regulation by light was impaired at 34°C, suggesting regulatory imbalances in this key phototrophic gene under supra-optimal warm conditions. Our findings highlight potential implications of growth temperature for regulating mechanisms of nutrient and energy acquisition, as well as the intracellular carbon flux in globally abundant marine photoheterotrophs.\u0000 </div>","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 3","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143554420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Resolving Symbiodiniaceae Diversity Across Coral Microhabitats and Reef Niches

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-03-04 DOI: 10.1111/1462-2920.70065

Wyatt C. Million, Christian R. Voolstra, Gabriela Perna, Giulia Puntin, Katherine Rowe, Maren Ziegler

{"title":"Resolving Symbiodiniaceae Diversity Across Coral Microhabitats and Reef Niches","authors":"Wyatt C. Million, Christian R. Voolstra, Gabriela Perna, Giulia Puntin, Katherine Rowe, Maren Ziegler","doi":"10.1111/1462-2920.70065","DOIUrl":"10.1111/1462-2920.70065","url":null,"abstract":"Dinoflagellates of the family Symbiodiniaceae are important symbionts of diverse marine animals and they also occupy different environmental niches on coral reefs. The link between diversity at ecosystem-scale to microhabitats of Symbiodiniaceae within the coral holobiont is largely unknown. Using ITS2-amplicon sequencing, we compared Symbiodiniaceae communities across four environments (seawater, near-reef vs. distant sediments and turf algae) and two coral microhabitats (tissue, mucus) on a coral reef in the Red Sea. We found that coral and environmental habitats were both dominated by the genera Symbiodinium, Cladocopium and Durusdinium, but environmental habitats additionally harboured Fugacium, Gerakladium and Halluxium. Each environmental habitat harboured a distinct Symbiodiniaceae community. Nonetheless, 17 ITS2 sequences were shared among coral and environmental habitats and were also part of nearly half of the ITS2 type profiles in coral-based communities. Tissues and mucus of 49 coral colonies from 17 genera had largely identical Symbiodiniaceae communities. Together with the large difference between environmental Symbiodiniaceae communities and those in the coral tissue and mucus, our results indicate a clear barrier between host-associated and environmental Symbiodiniaceae communities marked by only few shared complete type profiles. Monitoring coral colonies after mucus sampling confirmed its suitability for long-term monitoring of coral-associated Symbiodiniaceae communities.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 3","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70065","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143545921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Coexistence Theory for Microbial Ecology, and Vice Versa

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-03-03 DOI: 10.1111/1462-2920.70072

James A. Orr, David W. Armitage, Andrew D. Letten

{"title":"Coexistence Theory for Microbial Ecology, and Vice Versa","authors":"James A. Orr, David W. Armitage, Andrew D. Letten","doi":"10.1111/1462-2920.70072","DOIUrl":"https://doi.org/10.1111/1462-2920.70072","url":null,"abstract":"Classical models from theoretical ecology are seeing increasing uptake in microbial ecology, but there remains rich potential for closer cross-pollination. Here we explore opportunities for stronger integration of ecological theory into microbial research (and vice versa) through the lens of so-called “modern” coexistence theory. Coexistence theory can be used to disentangle the contributions different mechanisms (e.g., resource partitioning, environmental variability) make to species coexistence. We begin with a short primer on the fundamental concepts of coexistence theory, with an emphasis on the relevance to microbial communities. We next present a systematic review, which highlights the paucity of empirical applications of coexistence theory in microbial systems. In light of this gap, we then identify and discuss ways in which: (i) coexistence theory can help to answer fundamental and applied questions in microbial ecology, particularly in spatio-temporally heterogeneous environments, and (ii) experimental microbial systems can be leveraged to validate and advance coexistence theory. Finally, we address several unique but often surmountable empirical challenges posed by microbial systems, as well as some conceptual limitations. Nevertheless, thoughtful integration of coexistence theory into microbial ecology presents a wealth of opportunities for the advancement of both theoretical and microbial ecology.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 3","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70072","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143535739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic Diversity and Anatoxin Profiles of Freshwater Benthic Cyanobacteria From Nova Scotia (Canada)

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-02-27 DOI: 10.1111/1462-2920.70067

Cecilio Valadez-Cano, Nicolas Tromas, Adrian Reyes-Prieto, Lindsay Johnston, Yannan Huang, Hannah Morris, Lydia Zamlynny, Daniel G. Beach, Rob C. Jamieson, Janice Lawrence

{"title":"Genetic Diversity and Anatoxin Profiles of Freshwater Benthic Cyanobacteria From Nova Scotia (Canada)","authors":"Cecilio Valadez-Cano, Nicolas Tromas, Adrian Reyes-Prieto, Lindsay Johnston, Yannan Huang, Hannah Morris, Lydia Zamlynny, Daniel G. Beach, Rob C. Jamieson, Janice Lawrence","doi":"10.1111/1462-2920.70067","DOIUrl":"https://doi.org/10.1111/1462-2920.70067","url":null,"abstract":"Some mat-forming cyanobacteria produce harmful cyanotoxins, yet benthic species remain understudied compared to planktonic counterparts. This study assesses the diversity, distribution and toxin production of mat-forming cyanobacteria across lentic and lotic systems in Nova Scotia, Canada. We documented greater cyanobacterial species richness in lentic environments, with six dominant species distributed into two major Microcoleus clades, five of which represent putative novel taxa. Two Microcoleus species with the genetic repertoire to produce anatoxins were prevalent. One has been previously reported in Canada, while the second represents a novel species found exclusively in an environment impacted by discharge from a water treatment plant. We observed variability in the gene clusters responsible for the biosynthesis of anatoxin-a and associated analogues (ATXs), including the discovery of a novel anaG variant with a ~ 1.7 kb insertion in a Microcoleus strain dominating homoanatoxin-producing mats. This extended anaG, encoding a polyketide synthase with an additional methyltransferase domain, coexists with shorter variants, leading to the production of a mixture of ATXs. These findings highlight the genetic diversity of benthic cyanobacteria in freshwater environments, with Microcoleus as the primary contributor to the production of ATXs in both lentic and lotic systems, underscoring their potential to produce harmful toxins.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 3","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70067","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143513554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0