Environmental microbiology最新文献_第2页

Centimetre scale functional dispersal limitation of freshwater copiotrophs 淡水共栖生物一厘米尺度的功能扩散限制。

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2024-08-11 DOI: 10.1111/1462-2920.16682

Marie Dannenmann, Alizée Le Moigne, Cyrill Hofer, Jakob Pernthaler

{"title":"Centimetre scale functional dispersal limitation of freshwater copiotrophs","authors":"Marie Dannenmann, Alizée Le Moigne, Cyrill Hofer, Jakob Pernthaler","doi":"10.1111/1462-2920.16682","DOIUrl":"10.1111/1462-2920.16682","url":null,"abstract":"The freshwater microbiome harbours numerous copiotrophic bacteria that rapidly respond to elevated substrate concentrations. We hypothesized that their high centimetre-scale beta diversity in lake water translates into pronounced metabolic variability, and that a large fraction of microbial ‘metabolic potential’ originates from point sources such as fragile organic aggregates. Three experiments were conducted in pre-alpine Lake Zurich over the course of a harmful cyanobacterial bloom: Spatially explicit 9 ml ‘syringe’ samples were collected in situ at centimetre distances along with equally sized ‘mixed’ samples drawn from pre-homogenized lake water and incubated in BIOLOG EcoPlate substrate arrays. Fewer compounds promoted bacterial growth in the syringe than in the mixed samples, in particular during the pre- and late bloom periods. Community analysis of enrichments on three frequently utilized substrates revealed both pronounced heterogeneity and functional redundancy. Bacterial consortia had higher richness in mixed than in syringe samples and differed in composition. Members of the Enterobacter cloacae complex dominated the EcoPlate assemblages during the mid-bloom period irrespective of treatment or substrate. We conclude that small-scale functional dispersal limitation among free-living copiotrophs in lake water reduces local biotransformation potential, and that lacustrine blooms of harmful cyanobacteria can be environmental reservoirs for metabolically versatile potential pathogens.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":null,"pages":null},"PeriodicalIF":4.3,"publicationDate":"2024-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.16682","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141916405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Virus ecology and 7-year temporal dynamics across a permafrost thaw gradient 永冻土融化梯度上的病毒生态学和 7 年时间动态。

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2024-08-05 DOI: 10.1111/1462-2920.16665

Christine L. Sun, Akbar Adjie Pratama, Maria Consuelo Gazitúa, Dylan Cronin, Bridget B. McGivern, James M. Wainaina, Dean R. Vik, Ahmed A. Zayed, Benjamin Bolduc, IsoGenie Project Field Teams 2010-2017, the IsoGenie and EMERGE Projects Coordinators, Kelly C. Wrighton, Virginia I. Rich, Matthew B. Sullivan

{"title":"Virus ecology and 7-year temporal dynamics across a permafrost thaw gradient","authors":"Christine L. Sun, Akbar Adjie Pratama, Maria Consuelo Gazitúa, Dylan Cronin, Bridget B. McGivern, James M. Wainaina, Dean R. Vik, Ahmed A. Zayed, Benjamin Bolduc, IsoGenie Project Field Teams 2010-2017, the IsoGenie and EMERGE Projects Coordinators, Kelly C. Wrighton, Virginia I. Rich, Matthew B. Sullivan","doi":"10.1111/1462-2920.16665","DOIUrl":"10.1111/1462-2920.16665","url":null,"abstract":"Soil microorganisms are pivotal in the global carbon cycle, but the viruses that affect them and their impact on ecosystems are less understood. In this study, we explored the diversity, dynamics, and ecology of soil viruses through 379 metagenomes collected annually from 2010 to 2017. These samples spanned the seasonally thawed active layer of a permafrost thaw gradient, which included palsa, bog, and fen habitats. We identified 5051 virus operational taxonomic units (vOTUs), doubling the known viruses for this site. These vOTUs were largely ephemeral within habitats, suggesting a turnover at the vOTU level from year to year. While the diversity varied by thaw stage and depth-related patterns were specific to each habitat, the virus communities did not significantly change over time. The abundance ratios of virus to host at the phylum level did not show consistent trends across the thaw gradient, depth, or time. To assess potential ecosystem impacts, we predicted hosts in silico and found viruses linked to microbial lineages involved in the carbon cycle, such as methanotrophy and methanogenesis. This included the identification of viruses of Candidatus Methanoflorens, a significant global methane contributor. We also detected a variety of potential auxiliary metabolic genes, including 24 carbon-degrading glycoside hydrolases, six of which are uniquely terrestrial. In conclusion, these long-term observations enhance our understanding of soil viruses in the context of climate-relevant processes and provide opportunities to explore their role in terrestrial carbon cycling.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":null,"pages":null},"PeriodicalIF":4.3,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.16665","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141888839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A mesophilic relative of common glacier algae, Ancylonema palustre sp. nov., provides insights into the induction of vacuolar pigments in zygnematophytes 一种常见冰川藻类的嗜中性亲缘植物 Ancylonema palustre sp.

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2024-07-30 DOI: 10.1111/1462-2920.16680

Anna Busch, Emilia Slominski, Daniel Remias, Lenka Procházková, Sebastian Hess

{"title":"A mesophilic relative of common glacier algae, Ancylonema palustre sp. nov., provides insights into the induction of vacuolar pigments in zygnematophytes","authors":"Anna Busch, Emilia Slominski, Daniel Remias, Lenka Procházková, Sebastian Hess","doi":"10.1111/1462-2920.16680","DOIUrl":"10.1111/1462-2920.16680","url":null,"abstract":"The green algae of the genus Ancylonema, which belong to the zygnematophytes, are prevalent colonizers of glaciers worldwide. They display a striking reddish-brown pigmentation in their natural environment, due to vacuolar compounds related to gallic acid. This pigmentation causes glacier darkening when these algae bloom, leading to increased melting rates. The Ancylonema species known so far are true psychrophiles, which hinders experimental work and limits our understanding of these algae. For instance, the biosynthesis, triggering factors, and biological function of Ancylonema's secondary pigments remain unknown. In this study, we introduce a mesophilic Ancylonema species, A. palustre sp. nov., from temperate moorlands. This species forms the sister lineage to all known psychrophilic strains. Despite its morphological similarity to the latter, it exhibits unique autecological and photophysiological characteristics. It allows us to describe vegetative and sexual cellular processes in great detail. We also conducted experimental tests for abiotic factors that induce the secondary pigments of zygnematophytes. We found that low nutrient conditions combined with ultraviolet B radiation result in vacuolar pigmentation, suggesting a sunscreen function. Our thriving, bacteria-free cultures of Ancylonema palustre will enable comparative genomic studies of mesophilic and extremophilic zygnematophytes. These studies may provide insights into how Ancylonema species colonized the world's glaciers.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":null,"pages":null},"PeriodicalIF":4.3,"publicationDate":"2024-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.16680","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141855239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microdiversity in marine pelagic ammonia-oxidizing archaeal populations in a Mediterranean long-read metagenome 地中海长读数元基因组中海洋浮游氨氧化古细菌种群的微观多样性。

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2024-07-30 DOI: 10.1111/1462-2920.16684

Pablo Suárez-Moo, Jose M. Haro-Moreno, Francisco Rodriguez-Valera

{"title":"Microdiversity in marine pelagic ammonia-oxidizing archaeal populations in a Mediterranean long-read metagenome","authors":"Pablo Suárez-Moo, Jose M. Haro-Moreno, Francisco Rodriguez-Valera","doi":"10.1111/1462-2920.16684","DOIUrl":"10.1111/1462-2920.16684","url":null,"abstract":"The knowledge of the different population-level processes operating within a species, and the genetic variability of the individual prokaryotic genomes, is key to understanding the adaptability of microbial populations. Here, we characterized the flexible genome of ammonia-oxidizing archaeal (AOA) populations using a metagenomic recruitment approach and long-read (PacBio HiFi) metagenomic sequencing. In the lower photic zone of the western Mediterranean Sea (75 m deep), the genomes Nitrosopelagicus brevis CN25 and Nitrosopumilus catalinensis SPOT1 had the highest recruitment values among available complete AOA genomes. They were used to analyse the diversity of flexible genes (variable from strain to strain) by examining the long-reads located within the flexible genomic islands (fGIs) identified by their under-recruitment. Both AOA genomes had a large fGI involved in the glycosylation of exposed structures, highly variable, and rich in glycosyltransferases. N. brevis had two fGIs related to the transport of phosphorus and ammonium respectively. N. catalinensis had fGIs involved in phosphorus transportation and metal uptake. A fGI5 previously reported as ‘unassigned function’ in N. brevis could be associated with defense. These findings demonstrate that the microdiversity of marine microbe populations, including AOA, can be effectively characterized using an approach that incorporates third-generation sequencing metagenomics.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":null,"pages":null},"PeriodicalIF":4.3,"publicationDate":"2024-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.16684","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141855240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transient, context-dependent fitness costs accompanying viral resistance in isolates of the marine microalga Micromonas sp. (class Mamiellophyceae) 海洋微藻小龙虾（Micromonas sp.，Mamiellophyceae 类）分离物的病毒抗性伴随着瞬时的、依赖环境的适应性成本。

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2024-07-30 DOI: 10.1111/1462-2920.16686

Anamica Bedi de Silva, Shawn W. Polson, Christopher R. Schvarcz, Grieg F. Steward, Kyle F. Edwards

{"title":"Transient, context-dependent fitness costs accompanying viral resistance in isolates of the marine microalga Micromonas sp. (class Mamiellophyceae)","authors":"Anamica Bedi de Silva, Shawn W. Polson, Christopher R. Schvarcz, Grieg F. Steward, Kyle F. Edwards","doi":"10.1111/1462-2920.16686","DOIUrl":"10.1111/1462-2920.16686","url":null,"abstract":"Marine microbes are important in biogeochemical cycling, but the nature and magnitude of their contributions are influenced by their associated viruses. In the presence of a lytic virus, cells that have evolved resistance to infection have an obvious fitness advantage over relatives that remain susceptible. However, susceptible cells remain extant in the wild, implying that the evolution of a fitness advantage in one dimension (virus resistance) must be accompanied by a fitness cost in another dimension. Identifying costs of resistance is challenging because fitness is context-dependent. We examined the context dependence of fitness costs in isolates of the picophytoplankton genus Micromonas and their co-occurring dsDNA viruses using experimental evolution. After generating 88 resistant lineages from two ancestral Micromonas strains, each challenged with one of four distinct viral strains, we found resistance led to a 46% decrease in mean growth rate under high irradiance and a 19% decrease under low. After a year in culture, the experimentally selected lines remained resistant, but fitness costs had attenuated. Our results suggest that the cost of resistance in Micromonas is dependent on environmental conditions and the duration of population adaptation, illustrating the dynamic nature of fitness costs of viral resistance among marine protists.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":null,"pages":null},"PeriodicalIF":4.3,"publicationDate":"2024-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141855241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assembly and comparative analyses of the Geosiphon pyriformis metagenome 组装和比较分析Geosiphon pyriformis元基因组。

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2024-07-26 DOI: 10.1111/1462-2920.16681

Essam Sorwar, Jordana Inacio Nascimento Oliveira, Mathu Malar C., Manuela Krüger, Nicolas Corradi

{"title":"Assembly and comparative analyses of the Geosiphon pyriformis metagenome","authors":"Essam Sorwar, Jordana Inacio Nascimento Oliveira, Mathu Malar C., Manuela Krüger, Nicolas Corradi","doi":"10.1111/1462-2920.16681","DOIUrl":"10.1111/1462-2920.16681","url":null,"abstract":"Geosiphon pyriformis, a representative of the fungal sub-phylum Glomeromycotina, is unique in its endosymbiosis with cyanobacteria within a fungal cell. This symbiotic relationship occurs in bladders containing nuclei of G. pyriformis, Mollicutes-like bacterial endosymbionts (MRE), and photosynthetically active and dividing cells of Nostoc punctiforme. Recent genome analyses have shed light on the biology of G. pyriformis, but the genome content and biology of its endosymbionts remain unexplored. To fill this gap, we gathered and examined metagenomic data from the bladders of G. pyriformis, where N. punctiforme and MRE are located. This ensures that our analyses are focused on the organs directly involved in the symbiosis. By comparing this data with the genetic information of related cyanobacteria and MREs from other species of Arbuscular Mycorrhizal Fungi, we aimed to reveal the genetic content of these organisms and understand how they interact at a genetic level to establish a symbiotic relationship. Our analyses uncovered significant gene expansions in the Nostoc endosymbiont, particularly in mobile elements and genes potentially involved in xenobiotic degradation. We also confirmed that the MRE of Glomeromycotina are monophyletic and possess a highly streamlined genome. These genomes show dramatic differences in both structure and content, including the presence of enzymes involved in environmental sensing and stress response.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":null,"pages":null},"PeriodicalIF":4.3,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141757897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome-based taxonomic analysis of the genus Pseudoalteromonas reveals heterotypic synonyms 基于基因组的假交替单胞菌属分类分析揭示了异型同物异名。

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2024-07-23 DOI: 10.1111/1462-2920.16672

Patrick Videau, Maximillian D. Shlafstein, David K. Oline, Scott A. Givan, Linda Fleet Chapman, Wendy K. Strangman, Richard L. Hahnke, Jimmy H. Saw, Blake Ushijima

{"title":"Genome-based taxonomic analysis of the genus Pseudoalteromonas reveals heterotypic synonyms","authors":"Patrick Videau, Maximillian D. Shlafstein, David K. Oline, Scott A. Givan, Linda Fleet Chapman, Wendy K. Strangman, Richard L. Hahnke, Jimmy H. Saw, Blake Ushijima","doi":"10.1111/1462-2920.16672","DOIUrl":"10.1111/1462-2920.16672","url":null,"abstract":"The Pseudoalteromonas genus comprises members that have been demonstrated to play significant ecological roles and produce enzymes, natural products, and activities that are beneficial to the environment and economy. A comprehensive evaluation of the genus revealed that the genomes of several Pseudoalteromonas species are highly similar to each other, exceeding species cutoff values. This evaluation involved determining and comparing the average nucleotide identity, in silico DNA–DNA hybridization, average amino acid identity, and the difference in G + C% between Pseudoalteromonas type strains with publicly available genomes. The genome of the Pseudoalteromonas elyakovii type strain was further assessed through additional sequencing and genomic comparisons to historical sequences. These findings suggest that six Pseudoalteromonas species, namely P. mariniglutinosa, P. donghaensis, P. maricaloris, P. elyakovii, P. profundi, and P. issachenkonii, should be reclassified as later heterotypic synonyms of the following validly published species: P. haloplanktis, P. lipolytica, P. flavipulchra, P. distincta, P. gelatinilytica, and P. tetraodonis. Furthermore, two names without valid standing, ‘P. telluritireducens’ and ‘P. spiralis’, should be associated with the validly published Pseudoalteromonas species P. agarivorans and P. tetraodonis, respectively.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":null,"pages":null},"PeriodicalIF":4.3,"publicationDate":"2024-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.16672","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141747820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vertical confinement enhances surface exploration in bacterial twitching motility 垂直限制增强了细菌抽动运动的表面探索能力。

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2024-07-22 DOI: 10.1111/1462-2920.16679

Xiao Chen, Rongjing Zhang, Junhua Yuan

{"title":"Vertical confinement enhances surface exploration in bacterial twitching motility","authors":"Xiao Chen, Rongjing Zhang, Junhua Yuan","doi":"10.1111/1462-2920.16679","DOIUrl":"10.1111/1462-2920.16679","url":null,"abstract":"Bacteria are often found in environments where space is limited, and they attach themselves to surfaces. One common form of movement on these surfaces is bacterial twitching motility, which is powered by the extension and retraction of type IV pili. Although twitching motility in unrestricted conditions has been extensively studied, the effects of spatial confinement on this behaviour are not well understood. In this study, we explored the diffusive properties of individual twitching Pseudomonas aeruginosa cells in spatially confined conditions. We achieved this by placing the bacteria between layers of agarose and glass, and then tracking the long-term twitching motility of individual cells. Interestingly, we found that while confinement reduced the immediate speed of twitching, it paradoxically increased diffusion. Through a combination of mechanical and geometrical analysis, as well as numerical simulations, we showed that this increase in diffusion could be attributed to mechanical factors. The constraint imposed by the agarose altered the diffusion pattern of the bacteria from normal to superdiffusion. These findings provide valuable insights into the motile behaviour of bacteria in confined environments.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":null,"pages":null},"PeriodicalIF":4.3,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141747821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

When the going gets tough, the tough get going—Novel bacterial AAA+ disaggregases provide extreme heat resistance 艰难困苦，玉汝于成--新型 AAA+ 细菌分解物具有极强的耐热性。

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2024-07-22 DOI: 10.1111/1462-2920.16677

Valentin Bohl, Axel Mogk

{"title":"When the going gets tough, the tough get going—Novel bacterial AAA+ disaggregases provide extreme heat resistance","authors":"Valentin Bohl, Axel Mogk","doi":"10.1111/1462-2920.16677","DOIUrl":"10.1111/1462-2920.16677","url":null,"abstract":"Heat stress can lead to protein misfolding and aggregation, potentially causing cell death due to the loss of essential proteins. Bacteria, being particularly exposed to environmental stress, are equipped with disaggregases that rescue these aggregated proteins. The bacterial Hsp70 chaperone DnaK and the ATPase associated with diverse cellular activities protein ClpB form the canonical disaggregase in bacteria. While this combination operates effectively during physiological heat stress, it is ineffective against massive aggregation caused by temperature-based sterilization protocols used in the food industry and clinics. This leaves bacteria unprotected against these thermal processes. However, bacteria that can withstand extreme, man-made stress conditions have emerged. These bacteria possess novel ATPase associated with diverse cellular activities disaggregases, ClpG and ClpL, which are key players in extreme heat resistance. These disaggregases, present in selected Gram-negative or Gram-positive bacteria, respectively, function superiorly by exhibiting increased thermal stability and enhanced threading power compared to DnaK/ClpB. This enables ClpG and ClpL to operate at extreme temperatures and process large and tight protein aggregates, thereby contributing to heat resistance. The genes for ClpG and ClpL are often encoded on mobile genomic islands or conjugative plasmids, allowing for their rapid spread among bacteria via horizontal gene transfer. This threatens the efficiency of sterilization protocols. In this review, we describe the various bacterial disaggregases identified to date, characterizing their commonalities and the specific features that enable these novel disaggregases to provide stress protection against extreme stress conditions.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":null,"pages":null},"PeriodicalIF":4.3,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.16677","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141747822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Environmental control and metabolic strategies of organic-matter-responsive bacterioplankton in the Weddell Sea (Antarctica) 威德尔海（南极洲）有机物质响应型浮游细菌的环境控制和代谢策略。

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2024-07-18 DOI: 10.1111/1462-2920.16675

Judith Piontek, Christiane Hassenrück, Birthe Zäncker, Klaus Jürgens

{"title":"Environmental control and metabolic strategies of organic-matter-responsive bacterioplankton in the Weddell Sea (Antarctica)","authors":"Judith Piontek, Christiane Hassenrück, Birthe Zäncker, Klaus Jürgens","doi":"10.1111/1462-2920.16675","DOIUrl":"10.1111/1462-2920.16675","url":null,"abstract":"Heterotrophic microbial communities play a significant role in driving carbon fluxes in marine ecosystems. Despite their importance, these communities remain understudied in remote polar oceans, which are known for their substantial contribution to the biological drawdown of atmospheric carbon dioxide. Our research focused on understanding the environmental factors and genetic makeup of key bacterial players involved in carbon remineralization in the Weddell Sea, including its coastal polynyas. Our experiments demonstrated that the combination of labile organic matter supply and temperature increase synergistically boosted bacterial growth. This suggests that, besides low seawater temperature, carbon limitation also hinders heterotrophic bacterial activity. Through the analysis of metagenome-assembled genomes, we discovered distinct genomic adaptation strategies in Bacteroidia and Gammaproteobacteria, both of which respond to organic matter. Both natural phytoplankton blooms and experimental addition of organic matter favoured Bacteroidia, which possess a large number of gene copies and a wide range of functional membrane transporters, glycoside hydrolases, and aminopeptidases. In contrast, the genomes of organic-matter-responsive Gammaproteobacteria were characterized by high densities of transcriptional regulators and transporters. Our findings suggest that bacterioplankton in the Weddell Sea, which respond to organic matter, employ metabolic strategies similar to those of their counterparts in temperate oceans. These strategies enable efficient growth at extremely low seawater temperatures, provided that organic carbon limitation is alleviated.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":null,"pages":null},"PeriodicalIF":4.3,"publicationDate":"2024-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.16675","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141632982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0