Environmental microbiology最新文献_第2页

Archaeal Signalling Networks—New Insights Into the Structure and Function of Histidine Kinases and Response Regulators of the Methanogenic Archaeon Methanosarcina acetivorans

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-01-31 DOI: 10.1111/1462-2920.70047

Nora F. K. Georgiev, Anne L. Andersson, Zoe Ruppe, Loriana Kattwinkel, Nicole Frankenberg-Dinkel

{"title":"Archaeal Signalling Networks—New Insights Into the Structure and Function of Histidine Kinases and Response Regulators of the Methanogenic Archaeon Methanosarcina acetivorans","authors":"Nora F. K. Georgiev, Anne L. Andersson, Zoe Ruppe, Loriana Kattwinkel, Nicole Frankenberg-Dinkel","doi":"10.1111/1462-2920.70047","DOIUrl":"10.1111/1462-2920.70047","url":null,"abstract":"The methanogenic archaeon Methanosarcina acetivorans has one of the largest known archaeal genomes. With 53 histidine kinases (HK), it also has the largest set of signal transduction systems. To gain insight into the hitherto not very well understood signal transduction in Archaea and M. acetivorans in particular, we have categorised the predicted HK into four types based on their H-box using an in silico analysis. Representatives of three types were recombinantly produced in Escherichia coli and purified by affinity chromatography. All investigated kinases showed ATP binding and hydrolysis. The MA_type 2 kinase, which lacks the classical H-box, showed no autokinase activity. Furthermore, we could show that M. acetivorans possesses an above-average number of response regulators (RR), consisting of only a REC domain (REC-only). Using the hybrid kinase MA4377 as an example we show that both intra-and intermolecular transphosphorylation to REC domains occur. These experiments are furthermore indicative of complex phosphorelay systems in M. acetivorans and suggest that REC-only proteins act as a central hub in signal transduction in M. acetivorans.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 2","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11784639/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143064685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adaptive Laboratory Evolution Uncovers Potential Role of a DNA Helicase Mutation in Torulaspora delbrueckii Increased Sulphite Resistance

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-01-31 DOI: 10.1111/1462-2920.70038

Carolina Osório, Ticiana Fernandes, Teresa Rito, Pedro Soares, Ricardo Franco-Duarte, Maria João Sousa

{"title":"Adaptive Laboratory Evolution Uncovers Potential Role of a DNA Helicase Mutation in Torulaspora delbrueckii Increased Sulphite Resistance","authors":"Carolina Osório, Ticiana Fernandes, Teresa Rito, Pedro Soares, Ricardo Franco-Duarte, Maria João Sousa","doi":"10.1111/1462-2920.70038","DOIUrl":"10.1111/1462-2920.70038","url":null,"abstract":"<div>\u0000 \u0000 Wine industry has faced pressure to innovate its products. Saccharomyces cerevisiae has been the traditional yeast for producing alcoholic beverages, but interest has shifted from the conventional S. cerevisiae to non-Saccharomyces yeasts for their biotechnological potential. Among these, Torulaspora delbrueckii is particularly notable for its ability to enrich wine with novel flavours. During winemaking, sulphites are added to suppress spoilage microorganisms, making sulphite tolerance a valuable characteristic of wine yeasts. Adaptive laboratory evolution in liquid and solid media improved sulphite resistance in two T. delbrueckii strains, achieving, in the best case, a fourfold increase from 0.50 to 2.00 mM of sodium metabisulphite, highlighting the potential of these evolve strains for winemaking applications. Genomic analysis revealed SNPs/InDels in all the strains, including a novel unique missense mutation common to the four evolved isolates, but absent from the parental strains, located in chromosome VIII (protein TDEL0H03170, homologue of S. cerevisiae MPH1). These genes code for a protein catalogued as an ATP-dependent DNA helicase, known for its role in maintaining genome stability by participating in DNA repair pathways. We propose that this valine-to-serine mutation, common to all the evolved isolates, helps the evolved strains repair sulphite-induced DNA damage more effectively.\u0000 </div>","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 2","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143064516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbiome Stability Is Linked to Acropora Coral Thermotolerance in Northwestern Philippines

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-01-31 DOI: 10.1111/1462-2920.70041

Jake Ivan P. Baquiran, John Bennedick Quijano, Madeleine J. H. van Oppen, Patrick C. Cabaitan, Peter L. Harrison, Cecilia Conaco

{"title":"Microbiome Stability Is Linked to Acropora Coral Thermotolerance in Northwestern Philippines","authors":"Jake Ivan P. Baquiran, John Bennedick Quijano, Madeleine J. H. van Oppen, Patrick C. Cabaitan, Peter L. Harrison, Cecilia Conaco","doi":"10.1111/1462-2920.70041","DOIUrl":"10.1111/1462-2920.70041","url":null,"abstract":"<div>\u0000 \u0000 Corals associate with a diverse community of prokaryotic symbionts that provide nutrition, antioxidants and other protective compounds to their host. However, the influence of microbes on coral thermotolerance remains understudied. Here, we examined the prokaryotic microbial communities associated with colonies of Acropora cf. tenuis that exhibit high or low thermotolerance upon exposure to 33°C (heated) relative to 29°C (control). Using 16S rRNA sequencing, we show that the microbial community structure of all A. cf. tenuis colonies was similar to each other at control temperature. Thermotolerant colonies, however, had relatively greater abundance of Endozoicomonas, Arcobacter, Bifidobacterium and Lactobacillus. At elevated temperature, only thermosensitive colonies showed a distinct shift in their microbiome, with an increase in Flavobacteriales, Rhodobacteraceae and Vibrio, accompanying a marked bleaching response. Functional prediction indicated that prokaryotic communities associated with thermotolerant corals were enriched for genes related to metabolism, while microbiomes of thermosensitive colonies were enriched for cell motility and antibiotic compound synthesis. These differences may contribute to the variable performance of thermotolerant and thermosensitive corals under thermal stress. Identification of microbial taxa correlated with thermotolerance provides insights into beneficial bacterial groups that could be used for microbiome engineering to support reef health in a changing climate.\u0000 </div>","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 2","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143064703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Water Quality and Land Use Shape Bacterial Communities Across 621 Canadian Lakes

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-01-27 DOI: 10.1111/1462-2920.70037

Vera E. Onana, Beatrix E. Beisner, David A. Walsh

{"title":"Water Quality and Land Use Shape Bacterial Communities Across 621 Canadian Lakes","authors":"Vera E. Onana, Beatrix E. Beisner, David A. Walsh","doi":"10.1111/1462-2920.70037","DOIUrl":"10.1111/1462-2920.70037","url":null,"abstract":"<div>\u0000 \u0000 Human activities such as agriculture and urban development are linked to water quality degradation. Canada represents a large and heterogeneous landscape of freshwater lakes, where variations in climate, geography and geology interact with land cover alteration to influence water quality differently across regions. In this study, we investigated the influence of water quality and land use on bacterial communities across 12 ecozones. At the pan-Canadian scale, total phosphorus (TP) was the most significant water quality variable influencing community structure, and the most pronounced shift was observed at 110 μg/L of TP, corresponding to the transition from eutrophic to hypereutrophic conditions. At the regional scale, water quality significantly explained bacterial community structure in all ecozones. In terms of land use effect, at the pan-Canadian scale, agriculture and, to a lesser extent, urbanisation were significant land use variables influencing community structure. Regionally, in ecozones characterised by extensive agriculture, this land cover variable was consistently significant in explaining community structure. Likewise, in extensively urbanised ecozones, urbanisation was consistently significant in explaining community structure. Overall, these results demonstrate that bacterial richness and community structure are influenced by water quality and shaped by agriculture and urban development in different ways.\u0000 </div>","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143044763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ring A Cleaving Beta-Diketone Hydrolase Is a Key Enzyme of Steroid Degradation in Anaerobic Bacteria

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-01-27 DOI: 10.1111/1462-2920.70034

Christian Jacoby, Lina Peller, Jana Wenzler, Monika Luttermann, Wolfgang Seiche, Bernhard Breit, Matthias Boll

{"title":"Ring A Cleaving Beta-Diketone Hydrolase Is a Key Enzyme of Steroid Degradation in Anaerobic Bacteria","authors":"Christian Jacoby, Lina Peller, Jana Wenzler, Monika Luttermann, Wolfgang Seiche, Bernhard Breit, Matthias Boll","doi":"10.1111/1462-2920.70034","DOIUrl":"10.1111/1462-2920.70034","url":null,"abstract":"Bacterial degradation of ubiquitous and persistent steroids such as steroid hormones is important for their removal from the environment. Initial studies of steroid degradation in anaerobic bacteria suggested that ring-cleaving hydrolases are involved in oxygen-independent sterane skeleton degradation. However, the enzymes involved in ring A cleavage of the common intermediate androsta-1,4-diene-3,17-dione have remained unknown. Here, we enriched a ring A hydrolase from cholesterol/nitrate grown Sterolibacterium denitrificans and from Escherichia coli after heterologous expression of its gene. This enzyme specifically cleaves the cyclic 1,3-diketone of the central degradation intermediate, androsta-1,3,17-trione to 1,17-dioxo-2,3-seco-androstan-3-oate (DSAO), a hallmark reaction of anaerobic steroid degradation. The highly conserved ring A hydrolase was identified in all known and many previously unknown steroid-degrading proteobacteria. Using enriched enzymes, we enzymatically produced DSAO from the chemically synthesised androsta-1-en-3,17-dione precursor, allowing the identification of subsequent metabolites involved in ring A degradation. The results obtained suggest the involvement of an additional hydrolase, an aldolase, and a β-oxidation-like cascade for complete ring A degradation to form the three-ring 5,10-seco-1,2,3,4-tetranorandrosta-5,17-dione. The results identified a key enzyme of anaerobic steroid degradation that may serve as a functional marker for monitoring steroid contaminant degradation at anoxic environmental sites.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70034","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143044201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characteristics of Phages and Their Interactions With Hosts in Anaerobic Reactors

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-01-27 DOI: 10.1111/1462-2920.70040

Yan Zeng, Xiaozhong Zhong, Yating Chen, Min Gou, Ke Yu, Yue-Qin Tang

{"title":"Characteristics of Phages and Their Interactions With Hosts in Anaerobic Reactors","authors":"Yan Zeng, Xiaozhong Zhong, Yating Chen, Min Gou, Ke Yu, Yue-Qin Tang","doi":"10.1111/1462-2920.70040","DOIUrl":"10.1111/1462-2920.70040","url":null,"abstract":"<div>\u0000 \u0000 Anaerobic digestion (AD) of organic wastes relies on the interaction and cooperation of various microorganisms. Phages are crucial components of the microbial community in AD systems, but their diversity and interactions with the prokaryotic populations are still inadequately comprehended. In this study, 2121 viral operational taxonomic units (vOTUs) were recovered from 12 anaerobic fatty acid–fed reactors. Notably, 63.1% of these vOTUs could not be assigned to any known family, revealing a substantial presence of uncharted phages specifically associated with AD environments. Over half of the vOTUs associated with hosts had the capability to infect multiple hosts, ranging from 2 to 49, with a prevalent tendency to infect 2–5 hosts. In silico predictions of phage-host linkages uncovered that only a small fraction of vOTUs were shared across different functional groups, including fermentative bacteria, syntrophic fatty acid–oxidising bacteria (SFOB) and methanogens. Phages linked to hosts in all three groups primarily consisted of generalists and temperate species, especially those linked to SFOB. Additionally, metabolic reconstruction identified auxiliary metabolic genes participating in fatty acid degradation, methanogenesis and energy conservation. The present study provides insights into phage characteristics and their in situ interactions with prokaryotic hosts, highlighting their ecological role in AD systems.\u0000 </div>","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143044200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antarctic Geothermal Soils Exhibit an Absence of Regional Habitat Generalist Microorganisms

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-01-27 DOI: 10.1111/1462-2920.70032

Stephen E. Noell, Jaber Abbaszadeh, Huw Richards, Marie Labat Saint Vincent, Charles K. Lee, Craig W. Herbold, Matthew B. Stott, S. Craig Cary, Ian R. McDonald

{"title":"Antarctic Geothermal Soils Exhibit an Absence of Regional Habitat Generalist Microorganisms","authors":"Stephen E. Noell, Jaber Abbaszadeh, Huw Richards, Marie Labat Saint Vincent, Charles K. Lee, Craig W. Herbold, Matthew B. Stott, S. Craig Cary, Ian R. McDonald","doi":"10.1111/1462-2920.70032","DOIUrl":"10.1111/1462-2920.70032","url":null,"abstract":"Active geothermal systems are relatively rare in Antarctica and represent metaphorical islands ideal to study microbial dispersal. In this study, we tested the macro-ecological concept that high dispersal rates result in communities being dominated by either habitat generalists or specialists by investigating the microbial communities on four geographically separated geothermal sites on three Antarctic volcanoes (Mts. Erebus, Melbourne, and Rittman). We found that the microbial communities at higher temperature (max 65°C) sites (Tramway Ridge on Erebus and Rittmann) were unique from each other and were dominated by a variety of novel Archaea from class Nitrososphaeria, while lower temperature (max 50°C) sites (Western Crater on Erebus and Melbourne) had characteristically mesophilic communities (Planctomycetes, Acidobacteriota, etc.) that were highly similar. We found that 97% of the detected microbial taxa were regional habitat specialists, with no generalists, with community assembly driven by high dispersal rates and drift (25% and 30% of community assembly, respectively), not environmental selection. Our results indicate that for microbial communities experiencing high dispersal rates between isolated communities, habitat specialists may tend to out-compete habitat generalists.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70032","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143050867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evolutionary Novelties in Bacteria and the Missing Backdrop of the Environment

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-01-27 DOI: 10.1111/1462-2920.70044

Shraddha Karve

{"title":"Evolutionary Novelties in Bacteria and the Missing Backdrop of the Environment","authors":"Shraddha Karve","doi":"10.1111/1462-2920.70044","DOIUrl":"10.1111/1462-2920.70044","url":null,"abstract":"<div>\u0000 \u0000 Evolutionary novelty has been one of the central themes in the field of evolutionary biology for many years. Structural and functional innovations such as scales in the reptiles, fins in the fishes and mammary glands in the mammals have been the focus of the studies. Insights obtained from these studies have shaped the criterion for the identification of novelty as well as provide the framework for studying novelty. In this article, I argue that unicellular organisms present an excellent opportunity for the investigation of evolutionary novelty. Even though bacteria share some fundamental aspects of novelty with higher organisms, there are definite departures. Here, I outline these departures in four different contexts: criterion for the identification of novelty, types of evolutionary novelties, level of biological complexity that bacteria embody and, most importantly, the role of the environment. Identifying the role of the environment allows the categorisation of novelty as probable or improbable and adaptive or latent. This categorisation of novel traits, based on the role of the environment, can facilitate the study of novelty in bacteria. Insights obtained from such studies are crucial for understanding the fundamental aspects of evolutionary novelty.\u0000 </div>","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143044202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulation of Rhizobial Nodulation Genes by Flavonoid-Independent NodD Supports Nitrogen-Fixing Symbioses With Legumes

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-01-25 DOI: 10.1111/1462-2920.70014

Timothy L. Haskett, Louise Cooke, Patrick Green, Philip S. Poole

{"title":"Regulation of Rhizobial Nodulation Genes by Flavonoid-Independent NodD Supports Nitrogen-Fixing Symbioses With Legumes","authors":"Timothy L. Haskett, Louise Cooke, Patrick Green, Philip S. Poole","doi":"10.1111/1462-2920.70014","DOIUrl":"10.1111/1462-2920.70014","url":null,"abstract":"Rhizobia and legumes form a symbiotic relationship resulting in the formation of root structures known as nodules, where bacteria fix nitrogen. Legumes release flavonoids that are detected by the rhizobial nodulation (Nod) protein NodD, initiating the transcriptional activation of nod genes and subsequent synthesis of Nod Factors (NFs). NFs then induce various legume responses essential for this symbiosis. Although evidence suggests differential regulation of nodD expression and NF biosynthesis during symbiosis, the necessity of this regulation for the formation of nitrogen-fixing nodules remains uncertain. Here, we demonstrate that deletion of the Rlv3841 NodD regulatory domain results in a constitutively active protein (NodDFI) capable of activating NF biosynthesis gene expression without the presence of flavonoids. Optimised constitutive expression of nodDFI or nodD3 in nodD null mutants led to wild-type levels of nodulation and nitrogen fixation in pea and M. truncatula, respectively, indicating that flavonoid-regulated nodD expression is not essential for supporting symbiosis. These findings illustrate that transcriptional control of flavonoid-independent NodD regulators can be employed to drive NF biosynthesis, which holds potential for engineering symbiosis between rhizobia and cereals equipped with reconstituted NF receptors.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70014","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143030936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heat-Evolved Microalgae (Symbiodiniaceae) Are Stable Symbionts and Influence Thermal Tolerance of the Sea Anemone Exaiptasia diaphana 热演化微藻（共生藻科）是稳定的共生体并影响海葵耐热性

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-01-21 DOI: 10.1111/1462-2920.70011

Wing Yan Chan, Rumi Sakamoto, Talisa Doering, Vinod K. Narayana, David P. De Souza, Malcolm J. McConville, Madeleine J. H. van Oppen

{"title":"Heat-Evolved Microalgae (Symbiodiniaceae) Are Stable Symbionts and Influence Thermal Tolerance of the Sea Anemone Exaiptasia diaphana","authors":"Wing Yan Chan, Rumi Sakamoto, Talisa Doering, Vinod K. Narayana, David P. De Souza, Malcolm J. McConville, Madeleine J. H. van Oppen","doi":"10.1111/1462-2920.70011","DOIUrl":"10.1111/1462-2920.70011","url":null,"abstract":"Symbiotic cnidarians, such as sea anemones and corals, rely on their mutualistic microalgal partners (Symbiodiniaceae) for survival. Marine heatwaves can disrupt this partnership, and it has been proposed that introducing experimentally evolved, heat-tolerant algal symbionts could enhance host thermotolerance. To test this hypothesis, the sea anemone Exaiptasia diaphana (a coral model) was inoculated with either the heterologous wild type or heat-evolved algal symbiont, Cladocopium proliferum, and homologous wild-type Breviolum minutum. The novel symbioses persisted for 1.5 years and determined holobiont thermotolerance during a simulated summer heatwave. Anemones hosting SS8, one of the six heat-evolved strains tested, exhibited the highest thermotolerance. Notably, anemones hosting the wild-type C. proliferum (WT10) were the second most thermally tolerant group, whereas anemones hosting the heat-evolved SS5 or SS9 strains were among the most thermosensitive. Elevated temperatures led to an increase in the levels of many amino acids and a decrease in tricarboxylic acid (TCA) metabolites in all anemone hosts, potentially indicating an increase in autophagy and a reduction in energy and storage production. Some consistent differences were observed in changes in metabolite levels between anemone groups in response to elevated temperature, suggesting that the algal symbiont influenced host metabolome and nutritional budget.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70011","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142991912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0