Environmental microbiology最新文献_第3页

Long-Term Impact of the Largest Environmental Disaster in Latin America (Fundão Dam Failure) on Microbial Communities in Lakes of the Doce River Basin, Brazil 拉丁美洲最大的环境灾害（fund<e:1> o大坝溃坝）对巴西多塞河流域湖泊微生物群落的长期影响

IF 4 2区生物学

Environmental microbiology Pub Date : 2025-09-01 DOI: 10.1111/1462-2920.70171

Pedro Almeida, André Torres, Marcelos Gomes, Ernesto Caffarena, Hugo Jesus, Pedro Pereira, Katariny Pereira Dos Santos, Carlos Eduardo Delfino Vieira, Yuri Dornelles Zebral, Camila Martins, Adalto Bianchini, Henrique Santos

{"title":"Long-Term Impact of the Largest Environmental Disaster in Latin America (Fundão Dam Failure) on Microbial Communities in Lakes of the Doce River Basin, Brazil","authors":"Pedro Almeida, André Torres, Marcelos Gomes, Ernesto Caffarena, Hugo Jesus, Pedro Pereira, Katariny Pereira Dos Santos, Carlos Eduardo Delfino Vieira, Yuri Dornelles Zebral, Camila Martins, Adalto Bianchini, Henrique Santos","doi":"10.1111/1462-2920.70171","DOIUrl":"10.1111/1462-2920.70171","url":null,"abstract":"The collapse of the Fundão tailings dam in 2015 stands as the largest environmental disaster in Latin America and the global mining industry. This catastrophic event released around 62 million m3 of mining waste into the Doce River basin. This study aimed to assess the long-term effects of the disaster by analysing microbial communities in four lakes within the Doce River basin. Conducted over 4 years (2018–2021), with a total of six sampling campaigns. The microbiome of water and sediment was analysed using high-throughput 16S rRNA gene sequencing. The results demonstrate a significant correlation between key microbial groups and metals associated with the disaster, including Deinococcus, Thermoanaerobaculaceae, Pirellula, and Rhodospirillaceae. Moreover, an enrichment of genes associated with xenobiotic degradation and metal metabolism pathways was detected. These findings suggest that microbial communities in the lakes remain functionally adapted to metal contamination, potentially playing a crucial role in ecosystem recovery and bioremediation. These microorganisms could be leveraged to monitor and mitigate the effects of heavy metal contamination. Despite years having passed since the disaster, the microbiota of the lakes remains significantly impacted, reinforcing the need for continued research to fully understand and mitigate long-term ecological consequences.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 9","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://enviromicro-journals.onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70171","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144923472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Uncovering the Role of Land Use Intensity in Shaping Forest and Grassland-Specific Soil Fungal Communities 揭示土地利用强度对森林和草地土壤真菌群落形成的作用

IF 4 2区生物学

Environmental microbiology Pub Date : 2025-08-22 DOI: 10.1111/1462-2920.70170

Rosario Iacono, François Buscot, Spaska Forteva, Ingo Schöning, Marion Schrumpf, Emily Solly, Stephan Wöllauer, Kezia Goldmann

{"title":"Uncovering the Role of Land Use Intensity in Shaping Forest and Grassland-Specific Soil Fungal Communities","authors":"Rosario Iacono, François Buscot, Spaska Forteva, Ingo Schöning, Marion Schrumpf, Emily Solly, Stephan Wöllauer, Kezia Goldmann","doi":"10.1111/1462-2920.70170","DOIUrl":"10.1111/1462-2920.70170","url":null,"abstract":"Soil fungal communities are shaped by land use intensity (LUI) and environmental conditions, but their combined effects remain unclear. Using data from 300 forest and grassland plots across Germany from 2021, we analysed fungal taxa relative abundance and associations with environmental variables. Soil conditions, soil fungal diversity, and community composition were linked to ecosystem variables and differed significantly across LUI levels. Forests showed greater variation in soil conditions across LUI levels; grasslands displayed the most pronounced differences in fungal diversity. In forest ecosystems, taxa belonging to the classes Leotiomycetes and Sordariomycetes (all Pezizomycotina) were indicators under both high and low LUI levels (R > 0.55, p < 0.005). High LUI in forests was characterised by a higher ratio between Basidiomycota and Ascomycota. In grasslands, high LUI levels were associated with more indicator taxa from Mortierellomycota and fewer from Glomeromycota compared to low LUI levels (R > 0.6, p < 0.005). This is the first comprehensive study addressing differences in soil fungal communities between grasslands and forests and across management intensities in Europe. Our findings suggest differential response of the two ecosystems to changes in LUI, with forests having more resilient traits in terms of soil fungal community richness and composition, while grassland fungi appear more sensitive to management practices.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 8","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://enviromicro-journals.onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70170","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144888333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Sulphate-Reducing Bacteria Mixed-Species Biofilms on Microbiologically Influenced Corrosion 硫酸盐还原菌混合种生物膜对微生物影响腐蚀的影响

IF 4 2区生物学

Environmental microbiology Pub Date : 2025-08-20 DOI: 10.1111/1462-2920.70116

Liam Jones, Maria Salta, Torben Lund Skovhus, Kathryn Thomas, Timothy Illson, Julian Wharton, Jeremy Webb

{"title":"Effects of Sulphate-Reducing Bacteria Mixed-Species Biofilms on Microbiologically Influenced Corrosion","authors":"Liam Jones, Maria Salta, Torben Lund Skovhus, Kathryn Thomas, Timothy Illson, Julian Wharton, Jeremy Webb","doi":"10.1111/1462-2920.70116","DOIUrl":"10.1111/1462-2920.70116","url":null,"abstract":"Sulphate reducing prokaryotes are widely acknowledged as key contributors to microbiologically influenced corrosion in industry. Characterisation of their behaviour within mixed-species biofilms that reflect ecologically relevant conditions is limited. A novel dual anaerobic biofilm reactor protocol allowed a complex microbial consortium to be investigated. Continual biofilm growth resulted in significantly greater corrosive pit density, with 15 and 47 pits mm−2 in the biotic reactor for as received (AR) and polished (P) coupons respectively. There was an average pit density of 3 pits mm−2 in the abiotic reactor for both AR and P coupons. Moreover, a greater pit depth and size were observed when compared to the sterile abiotic conditions. Identifying and understanding the relative contributions of different microbial mechanisms within mixed-species biofilms is critical. Importantly, electroactive and corrosive Desulfovibrio desulfuricans and Desulfovibrio vulgaris were identified within the biofilm. These microorganisms play a crucial role in extracellular electron transfer, a key process in microbiologically influenced corrosion. The protocol not only deepens the mechanistic understanding of MIC but also offers a versatile tool for testing mitigation strategies under realistic and customizable conditions. This integrated approach can ultimately support the development of more targeted, sustainable corrosion prevention and management practices.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 8","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://enviromicro-journals.onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70116","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144869884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Global Review of Blue Carbon Ecosystem Microbial Communities 全球蓝碳生态系统微生物群落综述

IF 4 2区生物学

Environmental microbiology Pub Date : 2025-08-16 DOI: 10.1111/1462-2920.70168

Christina Birnbaum, Pawel Waryszak, Stacey M. Trevathan-Tackett, Jennifer L. Bowen, Rod M. Connolly, Carlos M. Duarte, Peter I. Macreadie

{"title":"Global Review of Blue Carbon Ecosystem Microbial Communities","authors":"Christina Birnbaum, Pawel Waryszak, Stacey M. Trevathan-Tackett, Jennifer L. Bowen, Rod M. Connolly, Carlos M. Duarte, Peter I. Macreadie","doi":"10.1111/1462-2920.70168","DOIUrl":"10.1111/1462-2920.70168","url":null,"abstract":"Microbial communities underpin biogeochemical processes in Blue Carbon Ecosystems (BCEs); however, a comprehensive review of geographic patterns in microbial diversity, microbial functions, and distribution is currently lacking. Here, for the first time, we have analysed 70 years (1930–2020) of archaeal, bacterial, and fungal diversity and functions in mangrove, saltmarsh, and seagrass ecosystems to elucidate publication and geographic trends in reporting data in BCEs and to identify knowledge gaps. Of the 649 journal articles analysed, research on BCE microbial communities has focused overwhelmingly on assessing bacterial richness and functions in BCEs. Our gap analysis revealed that only ~25%–50% of the countries that have BCEs have been represented, suggesting that our understanding of archaeal, bacterial, and fungal geographic diversity in BCEs is still incomplete. In the context of taxonomic-based limitations in our study's approach, we have identified gaps of knowledge in archaeal and fungal sediment biodiversity in saltmarsh and seagrass ecosystems. This significantly impacts our ability to forecast ecosystem services amid current and future human and climate pressures in BCEs. The results from this synthesis could serve as a useful reference for microbial baseline data and research trends in BCEs to develop novel hypothesis-testing research.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 8","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://enviromicro-journals.onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70168","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144853682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Low Temperature Enhances N-Metabolism in Paxillus involutus Mycelia In Vitro: Evidence From an Untargeted Metabolomic Study 低温促进体外对角Paxillus involutus菌丝体n代谢：来自非靶向代谢组学研究的证据

IF 4 2区生物学

Environmental microbiology Pub Date : 2025-08-12 DOI: 10.1111/1462-2920.70162

Agnieszka Szuba, Weronika B. Żukowska, Joanna Mucha, Aleksander Strugała, Łukasz Marczak

{"title":"Low Temperature Enhances N-Metabolism in Paxillus involutus Mycelia In Vitro: Evidence From an Untargeted Metabolomic Study","authors":"Agnieszka Szuba, Weronika B. Żukowska, Joanna Mucha, Aleksander Strugała, Łukasz Marczak","doi":"10.1111/1462-2920.70162","DOIUrl":"10.1111/1462-2920.70162","url":null,"abstract":"This metabolomic study investigates, using GC MS/MS analysis, the molecular response of Paxillus involutus mycelia to prolonged low temperature (4°C) exposure. Alongside reduced growth, decreased overall nutrient levels, and increased oxidative stress indicators, analyses revealed a significant increase in nitrogen (N) concentration and enhanced N metabolism, particularly via the GS–GOGAT pathway, which was associated with elevated concentrations of numerous amino acids. In contrast, carbon (C) metabolism was not intensified but largely reprogrammed, with varying changes in carbohydrate abundance but higher levels of several stress-related metabolites, such as trehalose and inositol family members, indicating activation of tolerance mechanisms, all with unchanged C (%). These changes suggest enhanced NH4+ uptake and a redirection of glycolysis-derived C skeletons towards N-compound biosynthesis. The lack of massive upregulation of typical anti-stress compounds under low temperature exposure indicates either acclimatisation or mild stress. Mycelial restructuring, including increased dry mass (%) and accumulation of chitin precursors, implies cell wall remodelling and cold acclimatisation, supported by changes in membrane components. All these findings suggest that low temperatures may enhance N metabolism in ECM fungi even without additional carbon supply, potentially affecting symbiotic balance under climate change. Further studies are needed to validate these mechanisms and ecological implications.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 8","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://enviromicro-journals.onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70162","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144832459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Air Plasmidome: A Discreet Route for Pathogens and the Transmission of Antibiotic Resistance Genes? 空气质粒：病原体和抗生素抗性基因传播的谨慎途径？

IF 4 2区生物学

Environmental microbiology Pub Date : 2025-08-10 DOI: 10.1111/1462-2920.70166

Didier Debroas, Claire Hennequin, Geneviève Bricheux

{"title":"The Air Plasmidome: A Discreet Route for Pathogens and the Transmission of Antibiotic Resistance Genes?","authors":"Didier Debroas, Claire Hennequin, Geneviève Bricheux","doi":"10.1111/1462-2920.70166","DOIUrl":"10.1111/1462-2920.70166","url":null,"abstract":"<div>\u0000 \u0000 Since the COVID-19 pandemic started, preventing airborne microorganism transmission has become everyone's concern. Viruses, bacteria, and fungal spores are airborne biological particles referring to bioaerosols. Through bacteria, a panel of genes (e.g., resistance or metabolic genes) might be found in bioaerosols and disseminated. Genes found on plasmids are potentially more exposed to the risk of transmissibility than those harboured by chromosomes. From 2422 air metagenomes analysed across various studies on bacteria, Proteobacteria were the most abundant microorganisms identified in air, with pathogens representing between 0.2% and 35.3% of the bacterial community per country. We identified 674,495 representative plasmids; 63,564 of them were linked to 5679 metagenome-assembled genomes. Among them, 89 plasmids were defined as keystones in terms of location numbers and plasmid coverage being more universal. Accessory traits of plasmids allow bacteria to adapt to their environment and contribute to host fitness. Antibiotic resistance genes represented < 1% of predicted plasmid genes and were mainly genes encoding efflux pumps. The plasmid similarity was significantly linked to the location and the microbial community (p < 0.001). Based on CRISPR detection, we determined that plasmids of bacteria coming from built environments and soils were overrepresented.\u0000 </div>","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 8","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144811087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Contrasting the Relative Importance of Microbial Generalists and Specialists in Maintaining Assembly Processes and Community Stability 比较微生物通才和专家在维持组装过程和群落稳定方面的相对重要性。

IF 4 2区生物学

Environmental microbiology Pub Date : 2025-08-09 DOI: 10.1111/1462-2920.70167

Xiaohong Niu, Xuming Sun, Yansong Bai, Na Wen, Xiao Wei, Gehong Wei, Duntao Shu

{"title":"Contrasting the Relative Importance of Microbial Generalists and Specialists in Maintaining Assembly Processes and Community Stability","authors":"Xiaohong Niu, Xuming Sun, Yansong Bai, Na Wen, Xiao Wei, Gehong Wei, Duntao Shu","doi":"10.1111/1462-2920.70167","DOIUrl":"10.1111/1462-2920.70167","url":null,"abstract":"<div>\u0000 \u0000 Microbial generalists and specialists play pivotal roles in community dynamics, yet their contributions to ecosystem stability under prolonged agricultural disturbance remain unclear. Here, to explore the different contributions of generalists and specialists to community stability, we collected 60 rhizosphere soil samples over three consecutive years (2021–2023) from long-term fertilised wheat fields on the Chinese Loess Plateau. We found that bacterial communities were dominated by generalists with higher microbial diversity, whereas fungal communities exhibited a contrasting pattern with a higher proportion of specialists. Bacterial generalists and specialists were predominantly shaped by homogeneous selection and dispersal limitation, respectively, while both fungal generalists and specialists were mainly governed by heterogeneous selection. We further found that soil NH4+ and available phosphorus (AP) mediated the balance between stochastic and deterministic assembly of generalists and specialists. Combined with network and community stability analysis, we found that generalists enhanced community stability through greater network robustness and species cohesion, whereas specialists substantially contributed by reinforcing network modularity and enhancing ecological adaptation to environmental changes. Our findings indicate the distinct importance of the generalists and specialists in regulating community stability, providing valuable guidance for understanding relationships between community assembly and ecosystem stability under anthropogenic disturbances.\u0000 </div>","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 8","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144802646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbiome of the Boring Giant Clam Provides Insights Into Holobiont Resilience Under Coral Reef Environmental Stress 钻孔巨蛤的微生物组提供了珊瑚礁环境压力下全息生物恢复力的见解

IF 4 2区生物学

Environmental microbiology Pub Date : 2025-08-06 DOI: 10.1111/1462-2920.70161

Taiga Uchida, Yanxiu Li, Hiroshi Yamashita, Go Shimada, Chuya Shinzato

{"title":"Microbiome of the Boring Giant Clam Provides Insights Into Holobiont Resilience Under Coral Reef Environmental Stress","authors":"Taiga Uchida, Yanxiu Li, Hiroshi Yamashita, Go Shimada, Chuya Shinzato","doi":"10.1111/1462-2920.70161","DOIUrl":"10.1111/1462-2920.70161","url":null,"abstract":"Giant clams are key denizens of coral reef ecosystems, forming holobionts through symbiotic relationships with algae of the family Symbiodiniaceae, as in reef-building corals. In this study, we performed a tissue-specific microbiome analysis of the boring giant clam, Tridacna crocea and evaluated the impact of dark-induced bleaching on its outer mantle bacterial community. Using 16S rRNA metabarcoding, Endozoicomonas was identified as the dominant bacterial genus in most tissues, particularly in gills, implying an important contribution to the giant clam holobiont. In contrast, in the outer mantle, where algal symbionts reside, the microbiome exhibited greater diversity, with a significant presence of carotenoid-producing bacteria such as Rubritalea (Rubritaleaceae) and Muricauda (Flavobacteriaceae). These bacteria may protect symbiotic algae from light and thermal stresses, potentially enhancing holobiont resilience in coral reef environments. Although dark-induced bleaching significantly reduced algal cell density, bacterial diversity remained largely unaffected, suggesting a robust bacterial association, independent of algal dynamics. This study highlights the potential ecological significance of Endozoicomonas and carotenoid-producing bacteria in sustaining giant clam holobiont health and provides insights into microbial mechanisms that support stress tolerance in coral reef organisms.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 8","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70161","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144782366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polyphosphate Dynamics in Marine Heterotrophic Bacteria Under Phosphorus and Organic Carbon Limitations 磷和有机碳限制下海洋异养细菌的多磷酸盐动态。

IF 4 2区生物学

Environmental microbiology Pub Date : 2025-08-06 DOI: 10.1111/1462-2920.70165

Rixuan Gao, Xingyu Yang, Qiong Zhang, Charmaine C. M. Yung, Ding He, Hongbin Yin, Jiying Li

{"title":"Polyphosphate Dynamics in Marine Heterotrophic Bacteria Under Phosphorus and Organic Carbon Limitations","authors":"Rixuan Gao, Xingyu Yang, Qiong Zhang, Charmaine C. M. Yung, Ding He, Hongbin Yin, Jiying Li","doi":"10.1111/1462-2920.70165","DOIUrl":"10.1111/1462-2920.70165","url":null,"abstract":"<div>\u0000 \u0000 Marine heterotrophic bacteria produce polyphosphate (polyP) ubiquitously, yet their polyP functions and ecological significance are rarely studied. We investigated polyP dynamics of three common marine bacteria under phosphorus (P) and organic carbon limitations. Our results show that these bacteria accumulate varying levels of polyP: Alteromonas sp. accumulates up to 87% of polyP in total biomass P, Photobacterium ganghwense accumulates up to 35%, and Vibrio sp. accumulates less than 16%. This variability appears linked to differences in polyP functions. Under P limitation, polyP supports the growth of the two high-polyP-accumulating species, but not in Vibrio sp. with a low polyP level. Under organic carbon limitation, P. ganghwense uniquely degrades polyP for energy and survival. However, this mechanism is not observed under P limitation, despite similar levels of polyP accumulation in the bacteria. The phosphate produced from polyP degradation in P. ganghwense under organic carbon limitation is recycled within cells rather than released into the environment. Overall, our findings suggest that polyP enables some marine heterotrophic bacteria to cope with P and organic carbon limitations, potentially enhancing their competitiveness against phytoplankton for the common limiting nutrient phosphorus in marine ecosystems.\u0000 </div>","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 8","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144791979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extracellular rRNA Profiling Reveals the Sinking and Cell Lysis Dynamics of Marine Microeukaryotes 胞外rRNA谱揭示了海洋微真核生物的下沉和细胞裂解动力学

IF 4 2区生物学

Environmental microbiology Pub Date : 2025-08-05 DOI: 10.1111/1462-2920.70164

Hisashi Endo, Yuki Yamagishi, Thi Tuyen Nguyen, Hiroyuki Ogata

{"title":"Extracellular rRNA Profiling Reveals the Sinking and Cell Lysis Dynamics of Marine Microeukaryotes","authors":"Hisashi Endo, Yuki Yamagishi, Thi Tuyen Nguyen, Hiroyuki Ogata","doi":"10.1111/1462-2920.70164","DOIUrl":"10.1111/1462-2920.70164","url":null,"abstract":"Marine plankton communities consist of numerous species, and their composition and physiological states are closely linked to ecosystem functions. Understanding biogeochemical cycles requires measuring taxon-specific mortality due to viral lysis, sloppy feeding, and other mechanical stresses as the dissolved organic matter released contributes to rapid nutrient recycling and long-term carbon sequestration following microbial transformation. To examine the lytic cell death of marine microeukaryotes, we applied a quantitative and comprehensive analysis of the dissolved constituents of seawater using the Mortality by Ribosomal Sequencing (MoRS) method. Our experimental pipeline successfully recovered 83% of cell-free rRNA. A higher number of protist phylotypes was significantly lysed in the mesopelagic zone than in the surface ecosystems, indicating that the mesopelagic zone is a potential hotspot for eukaryotic cell lysis. Many protist lineages, including phytoplankton such as haptophytes, were less susceptible to cell lysis in the epipelagic layer yet were actively lysed in the mesopelagic zone. Notably, over 86% of the significantly lysed species in the mesopelagic layer had a habitat preference for the epipelagic layer. These findings suggest that sinking from the surface and lysis in the mesopelagic may represent prevalent dynamics for various eukaryotes.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 8","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70164","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144782656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0