Environmental microbiology最新文献_第4页

Pseudomonas Species Isolated From Lotus Nodules Are Genetically Diverse and Promote Plant Growth

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-02-27 DOI: 10.1111/1462-2920.70066

Yu-Hsiang Yu, Julian Kurtenbach, Duncan Crosbie, Andreas Brachmann, Macarena Marín Arancibia

{"title":"Pseudomonas Species Isolated From Lotus Nodules Are Genetically Diverse and Promote Plant Growth","authors":"Yu-Hsiang Yu, Julian Kurtenbach, Duncan Crosbie, Andreas Brachmann, Macarena Marín Arancibia","doi":"10.1111/1462-2920.70066","DOIUrl":"https://doi.org/10.1111/1462-2920.70066","url":null,"abstract":"Nodules harbour microbial communities composed of rhizobia and other lower-abundance bacteria. These non-rhizobial bacteria can promote plant growth. However, their genomic diversity and how this relates to their plant growth-promoting traits remain poorly investigated. Here, we isolated 14 Pseudomonas strains from the nodules of Lotus plants, sequenced their genomes, analysed their genomic and phylogenetic diversity, and assessed their ability to promote plant growth. We identified five distinct species, including a novel species named Pseudomonas monachiensis sp. nov., with strain PLb12AT, as the type strain. Genome analysis of these nodule-isolated Pseudomonas revealed an abundance of genes associated to plant growth-promoting traits, especially auxin-related genes, compared to closely related type strains. In accordance, most nodule-isolated Pseudomonas strains enhanced shoot growth of Lotus burttii, while only some promoted root growth or early onset of root hair proliferation. However, none of the strains significantly affected the ability to form nodules. Overall, our findings highlight the genotypic diversity and the plant growth-promoting potential of nodule-isolated Pseudomonas and underscore their possible applications in mixed inocula with rhizobia.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 3","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70066","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143513727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Bacterial Antimonite Oxidase AnoA: Unexpected Diversity and Environmental Widespread Occurrence

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-02-26 DOI: 10.1111/1462-2920.70069

Claire Da Costa, Thierry Berthe, Titouan Dehaies, Sophie Ayrault, Yannick Colin

{"title":"The Bacterial Antimonite Oxidase AnoA: Unexpected Diversity and Environmental Widespread Occurrence","authors":"Claire Da Costa, Thierry Berthe, Titouan Dehaies, Sophie Ayrault, Yannick Colin","doi":"10.1111/1462-2920.70069","DOIUrl":"https://doi.org/10.1111/1462-2920.70069","url":null,"abstract":"<div>\u0000 \u0000 The growing contamination of urban areas by antimony (Sb) has sparked interest in microbial processes that modulate Sb speciation in ecosystems. The bacterial antimonite oxidase AnoA is the only oxidase known so far whose gene expression is specifically induced by Sb(III), but its annotation in public databases is currently lacking. Here, the computational search for AnoA orthologs predicted an unexpected phylogenetic distribution across the Pseudomonadota and Actinomycetota. Putative orthologs were identified in both known Sb(III)-oxidisers (e.g., Shinella, Hydrogenophaga, Bosea, Cupriavidus and Pseudomonas) and taxa not previously linked to the Sb cycle (e.g., Bradyrhizobium, Mesorhizobium, Methylobacterium and Paraburkholderia). The anoA gene is single-copy in most Proteobacterial genomes, but is often detected in multiple copies in the Actinomycetota. Furthermore, sequence evolutionary distances suggest that it is mainly inherited vertically, with horizontal transfer events, in particular towards the Gammaproteobacteria. Using the constructed database, new PCR primers were designed and outperformed existing strain-specific primers in amplifying the anoA gene from samples with varying Sb levels and microbial profiles. Sequencing and quantification of PCR amplicons revealed a diverse range of sequences in sediments and natural biofilms, indicating that the oxidase is more environmentally diverse and widespread than previously thought and may play a significant role in Sb(III) detoxification.\u0000 </div>","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 3","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143489993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Co-Existence Slows Diversification in Experimental Populations of E. coli and P. fluorescens

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-02-23 DOI: 10.1111/1462-2920.70061

Gareth Howells, Aysha L. Sezmis, Christopher Blake, Michael J. McDonald

{"title":"Co-Existence Slows Diversification in Experimental Populations of E. coli and P. fluorescens","authors":"Gareth Howells, Aysha L. Sezmis, Christopher Blake, Michael J. McDonald","doi":"10.1111/1462-2920.70061","DOIUrl":"https://doi.org/10.1111/1462-2920.70061","url":null,"abstract":"Microbes grown in heterogeneous laboratory environments can rapidly diversify into multiple, coexisting variants. While the genetic and evolutionary mechanisms of laboratory adaptive radiations are well studied, how the presence of other species alters the outcomes of diversification is less well understood. To test the effect of co-culture growth on the Pseudomonas fluorescens SBW25 adaptive radiation, Escherichia coli and P. fluorescens were cultured in monoculture and co-culture for 8 weeks. In P. fluorescens monoculture, Wrinkly and Smooth Spreader types rapidly evolved and were maintained over 8 weeks, while E. coli monocultures evolved two colony types, a big and a small colony variant. In contrast, we found that in co-culture, E. coli did not evolve small colony variants. Whole genome sequencing revealed the genetic basis of possible co-culture specific adaptations in both E. coli and P. fluorescens. Altogether, our data support that the presence of multiple species changed the outcome of adaptive radiation.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 2","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70061","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143475568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Bacterial Genus Ramlibacter: Betaproteobacteria Capable of Surviving in Oligotrophic Environments Thanks to Several Shared Genetic Adaptation Traits

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-02-23 DOI: 10.1111/1462-2920.70059

Gilles De Luca, Mohamed Barakat, André Verméglio, Wafa Achouak, Thierry Heulin

{"title":"The Bacterial Genus Ramlibacter: Betaproteobacteria Capable of Surviving in Oligotrophic Environments Thanks to Several Shared Genetic Adaptation Traits","authors":"Gilles De Luca, Mohamed Barakat, André Verméglio, Wafa Achouak, Thierry Heulin","doi":"10.1111/1462-2920.70059","DOIUrl":"https://doi.org/10.1111/1462-2920.70059","url":null,"abstract":"Ramlibacter tataouinensis, the type species of the genus Ramlibacter, is renowned for its ability to thrive in hot, arid and nutrient-poor desert soils. To investigate whether its adaptive properties are shared across all 20 currently described Ramlibacter species found in diverse terrestrial and aquatic habitats worldwide, we conducted a comprehensive analysis of 16S rRNA sequences and genomic information available from the literature. Our study encompassed approximately 40 deposited genomes, allowing us to propose a genomic phylogeny that aligns with the 16S rRNA phylogeny. Our findings reveal several conserved features across the genus Ramlibacter. This includes the presence of light sensors, environmental sensing networks, organic carbon and phosphate acquisition systems and the ability to store carbon and energy in the form of polyhydroxyalkanoate or polyphosphate granules. These shared traits rationalise the widespread distribution of Ramlibacter in oligotrophic terrestrial and aquatic environments. They also explain the genus' ability to withstand desiccation, endure extended periods of starvation, and survive in nutrient-depleted conditions. Notably, certain adaptive features are further enhanced in several species by their pleiomorphism and ability to form cysts. Overall, our study not only highlights the ecological adaptations of Ramlibacter species but also extends our understanding of microbial ecology in oligotrophic environments.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 2","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70059","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143475567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Contrasting Methane, Sulfide and Nitrogen-Loading Regimes in Bioreactors Shape Microbial Communities Originating From Methane-Rich Coastal Sediment of the Stockholm Archipelago

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-02-16 DOI: 10.1111/1462-2920.70056

Maider J. Echeveste Medrano, Garrett J. Smith, Irene Sánchez-Andrea, Mike S. M. Jetten, Cornelia U. Welte

{"title":"Contrasting Methane, Sulfide and Nitrogen-Loading Regimes in Bioreactors Shape Microbial Communities Originating From Methane-Rich Coastal Sediment of the Stockholm Archipelago","authors":"Maider J. Echeveste Medrano, Garrett J. Smith, Irene Sánchez-Andrea, Mike S. M. Jetten, Cornelia U. Welte","doi":"10.1111/1462-2920.70056","DOIUrl":"https://doi.org/10.1111/1462-2920.70056","url":null,"abstract":"Coastal ecosystems are increasingly exposed to high nutrient loads and salinity intrusions due to rising seawater levels. Microbial communities, key drivers of elemental cycles in these ecosystems, consequently, experience fluctuations. This study investigates how the methane-rich coastal sediment microbiome from the Stockholm Archipelago copes with high and low nitrogen and sulfide loading by simulating coastal conditions in two methane-saturated anoxic brackish bioreactors. Over a year, the bioreactors were subjected to the same ratio of nitrate, ammonium and sulfide (2:1:1) under eutrophic or oligotrophic conditions and monitored using 16S rRNA gene amplicon and metagenomic sequencing. Sulfide was depleted in both conditions. Sulfide-dependent denitrification was the predominant process in eutrophic conditions, whereas dissimilatory nitrate reduction to ammonium dominated under oligotrophic conditions. Methane oxidation was driven by Methylobacter and Methylomonas in eutrophic conditions, whereas a more diverse methane-oxidising microbial community developed under oligotrophic conditions, which likely competed for nitrate with anaerobic methanotrophic archaea and the gammaproteobacterial MBAE14. Novel putative copper-dependent membrane-bound monooxygenases (Cu-MMOs) were identified in MBAE14 and co-enriched Rugosibacter genomes, suggesting the need for further physiological and genetic characterisation. This study highlights the importance of understanding coastal anoxic microbiomes under fluctuating conditions, revealing complex interactions and novel pathways crucial for ecosystem functioning.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 2","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70056","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143423751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Thermodynamics Underpinning the Microbial Community-Level Nitrogen Energy Metabolism

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-02-16 DOI: 10.1111/1462-2920.70055

Mayumi Seto, Risa Sasaki, Hideshi Ooka, Ryuhei Nakamura

{"title":"Thermodynamics Underpinning the Microbial Community-Level Nitrogen Energy Metabolism","authors":"Mayumi Seto, Risa Sasaki, Hideshi Ooka, Ryuhei Nakamura","doi":"10.1111/1462-2920.70055","DOIUrl":"https://doi.org/10.1111/1462-2920.70055","url":null,"abstract":"Nitrogen compounds often serve as crucial electron donors and acceptors in microbial energy metabolism, playing a key role in biogeochemical cycles. The energetic favorability of nitrogen oxidation–reduction (redox) reactions, driven by the thermodynamic properties of these compounds, may have shaped the evolution of microbial energy metabolism, though the extent of their influence remains unclear. This study quantitatively evaluated the similarity between energetically superior nitrogen reactions, identified from 988 theoretically plausible reactions, and the nitrogen community-level network, reconstructed as a combination of enzymatic reactions representing intracellular to interspecies-level reaction interactions. Our analysis revealed significant link overlap rates between these networks. Notably, composite enzymatic reactions aligned more closely with energetically superior reactions than individual enzymatic reactions. These findings suggest that selective pressure from the energetic favorability of redox reactions can operate primarily at the species or community level, underscoring the critical role of thermodynamics in shaping microbial metabolic networks and ecosystem functioning.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 2","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70055","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143423752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insects as Natural Hosts, Vectors and Reservoirs of Botulinum Neurotoxin-Producing Clostridia and Their Non-Toxinogenic Counterparts: Preliminary Evidence

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-02-13 DOI: 10.1111/1462-2920.70053

François P. Douillard, Olivia Lanzoni, Anne Duplouy, Miia Lindström

{"title":"Insects as Natural Hosts, Vectors and Reservoirs of Botulinum Neurotoxin-Producing Clostridia and Their Non-Toxinogenic Counterparts: Preliminary Evidence","authors":"François P. Douillard, Olivia Lanzoni, Anne Duplouy, Miia Lindström","doi":"10.1111/1462-2920.70053","DOIUrl":"https://doi.org/10.1111/1462-2920.70053","url":null,"abstract":"<div>\u0000 \u0000 Insects play a significant role in the transmission and spread of bacterial pathogens that cause various diseases in humans and animals. The relationship among insects, bacterial pathogens and diseases is complex and depends on the specificity of the pathogens. Some clostridial species produce botulinum neurotoxin (BoNT), which is responsible for paralytic botulism. However, the ecology of these bacterial species and their non-toxinogenic phylogenetic counterparts remains unclear. This study specifically explored in silico evidence of the interconnection between BoNT-producing Clostridia and their non-toxinogenic counterparts with insects. Based on literature meta-analysis, the mining of 16S rRNA amplicon and metagenomic sequencing datasets and a pilot feeding experiment in the Glanville fritillary butterfly, Melitaea cinxia, we propose that BoNT-producing Clostridia and their non-toxinogenic phylogenetic counterparts are carried internally and/or externally in different insect orders. While previous case studies have indicated associations between Clostridia and insects, this work provides a more comprehensive view of their occurrence. It also highlights the need for further multidisciplinary investigations to characterise the natural ecology of BoNT-producing Clostridia and their non-toxinogenic counterparts in insects.\u0000 </div>","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 2","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143404447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phylogenetic and Functional Diversity of Soluble Di-Iron Monooxygenases

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-02-13 DOI: 10.1111/1462-2920.70050

Sui Nin Nicholas Yang, Michael A. Kertesz, Nicholas V. Coleman

{"title":"Phylogenetic and Functional Diversity of Soluble Di-Iron Monooxygenases","authors":"Sui Nin Nicholas Yang, Michael A. Kertesz, Nicholas V. Coleman","doi":"10.1111/1462-2920.70050","DOIUrl":"https://doi.org/10.1111/1462-2920.70050","url":null,"abstract":"Monooxygenase (MO) enzymes are responsible for the oxidation of hydrocarbons and other compounds in the carbon and nitrogen cycles, are important for the biodegradation of pollutants and can act as biocatalysts for chemical manufacture. The soluble di-iron monooxygenases (SDIMOs) are of interest due to their broad substrate range, high enantioselectivity and ability to oxidise inert substrates such as methane. Here, we re-examine the phylogeny and functions of these enzymes, using recent advances in the field and expansions in sequence diversity in databases to highlight relationships between SDIMOs and revisit their classification. We discuss the impact of horizontal gene transfer on SDIMO phylogeny, the potential of SDIMOs for the biodegradation of pollutants and the importance of heterologous expression as a tool for understanding SDIMO functions and enabling their use as biocatalysts. Our analysis highlights current knowledge gaps, most notably, the unknown substrate ranges and physiological roles of enzymes that have so far only been detected via genome or metagenome sequencing. Enhanced understanding of the diversity and functions of the SDIMO enzymes will enable better prediction and management of biogeochemical processes and also enable new applications of these enzymes for biocatalysis and bioremediation.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 2","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70050","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143404445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Highly Dynamic Archaeal and Bacterial Communities From the Surface to the Deep in the Atlantic Ocean

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-02-13 DOI: 10.1111/1462-2920.70039

Kristin M. Yoshimura, Sunita R. Shah Walter, Benjamin J. Tully, Jennifer F. Biddle

{"title":"Highly Dynamic Archaeal and Bacterial Communities From the Surface to the Deep in the Atlantic Ocean","authors":"Kristin M. Yoshimura, Sunita R. Shah Walter, Benjamin J. Tully, Jennifer F. Biddle","doi":"10.1111/1462-2920.70039","DOIUrl":"https://doi.org/10.1111/1462-2920.70039","url":null,"abstract":"<div>\u0000 \u0000 Though archaea are ubiquitous in the oceans, at times present in quantities equal to bacteria, in general, archaea are underreported when compared with bacteria, and little is known about archaeal connectivity in the ocean. Here, we present the first time series data of size-fractionated archaea with temporal resolution of days across a depth transect in the oligotrophic Atlantic Ocean. Archaeal communities were variable from day to day, both in regards to community composition and relative abundance. In the context of the total prokaryotic community, we found that water depth and particle size were both determinants of the archaeal share of microbial populations, though the archaeal portion of the community was less discriminating between size and depth classes than the rest of the community. Quantitative PCR shows lower relative abundances of archaea in the deep Atlantic Ocean than previously reported. Overall, we find far lower connectivity between depths in the Atlantic Ocean than suggested by previous studies.\u0000 </div>","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 2","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143404446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Invertebrate Decline Has Minimal Effects on Oak-Associated Microbiomes 无脊椎动物的减少对橡树相关微生物群的影响微乎其微

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-02-12 DOI: 10.1111/1462-2920.70051

Cynthia Albracht, François Buscot, Nico Eisenhauer, Alban Gebler, Sylvie Herrmann, Anja Schmidt, Mika Tarkka, Kezia Goldmann

{"title":"Invertebrate Decline Has Minimal Effects on Oak-Associated Microbiomes","authors":"Cynthia Albracht, François Buscot, Nico Eisenhauer, Alban Gebler, Sylvie Herrmann, Anja Schmidt, Mika Tarkka, Kezia Goldmann","doi":"10.1111/1462-2920.70051","DOIUrl":"https://doi.org/10.1111/1462-2920.70051","url":null,"abstract":"Recently, biomass of invertebrates has declined substantially at many locations with the implications of this biodiversity loss for ecosystems yet unknown. Through multitrophic interactions, plant- and soil-associated microbiomes might be altered, causing a cascade of changes on diverse ecosystem processes. We simulated aboveground invertebrate decline in grassland ecosystems with two levels of invertebrate biomass (36% and 100% of current ambient conditions), plus a control with no invertebrates present. Each standardised grassland mesocosm additionally contained one clonal Quercus robur L. sapling to investigate the extent of invertebrate decline effects exceeding grasslands. We investigated oak biomass partitioning and mycorrhiza formation, oak leaf transcriptome and microbiome composition of leaves, roots and rhizosphere. While invertebrate decline did not significantly affect oak performance and herbivory-related gene expression, fungal communities presented an increase of saprotrophs and pathogens, especially in leaves. Among leaf-inhabiting bacteria, Proteobacteria and Actinobacteria increased under invertebrate decline. The belowground microbiome was only little affected. But, invertebrate decline came along with a reduced influence on predators leading to an elevated aphids infestation that proofed able to alter microbiota. Our findings establish a strong difference between above- and belowground, with the impacts of invertebrate decline being more pronounced in the leaf microbiome.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 2","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70051","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143396952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0