Environmental microbiology最新文献_第4页

Marine Community Metabolomes in the Eastern Tropical North Pacific Oxygen Deficient Zone Reveal Glycine Betaine as a Metabolic Link Between Prochlorococcus and SAR11 北太平洋东部热带缺氧区海洋群落代谢组学揭示甜菜碱是原绿球藻与SAR11之间的代谢联系

IF 4 2区生物学

Environmental microbiology Pub Date : 2025-08-05 DOI: 10.1111/1462-2920.70119

Natalie A. Kellogg, Clara A. Fuchsman, Laura T. Carlson, Robert M. Morris, Anitra E. Ingalls, Gabrielle Rocap

{"title":"Marine Community Metabolomes in the Eastern Tropical North Pacific Oxygen Deficient Zone Reveal Glycine Betaine as a Metabolic Link Between Prochlorococcus and SAR11","authors":"Natalie A. Kellogg, Clara A. Fuchsman, Laura T. Carlson, Robert M. Morris, Anitra E. Ingalls, Gabrielle Rocap","doi":"10.1111/1462-2920.70119","DOIUrl":"10.1111/1462-2920.70119","url":null,"abstract":"Oxygen deficient zones (ODZs) are subsurface marine systems that harbour distinct microbial communities, including populations of the picocyanobacteria Prochlorococcus that can form a secondary chlorophyll maximum (SCM), and low-oxygen tolerant strains of the globally abundant heterotroph Pelagibacter (SAR11). Yet, the small labile molecules (metabolites) responsible for maintaining these ODZ communities are unknown. Here, we compared the metabolome of an ODZ to that of an oxygenated site by quantifying 87 metabolites across depth profiles in the eastern tropical North Pacific ODZ and the oxygenated waters of the North Pacific Gyre. Metabolomes were largely consistent between anoxic and oxic water columns. However, the osmolyte glycine betaine (GBT) was enriched in the oxycline and SCM of the ETNP, comprising as much as 1.2% of particulate organic carbon. Transcriptomes revealed two active GBT production pathways, glycine methylation (SDMT/bsmB) expressed by Prochlorococcus and choline oxidation (betB) expressed by Gammaproteobacteria. GBT consumption through demethylation involved diverse microbial taxa, with SAR11 contributing nearly half of the transcripts for the initial step of GBT demethylation (BHMT), which is predicted to convert GBT and homocysteine into dimethylglycine and methionine, a compound SAR11 cannot otherwise produce. Thus, GBT connects the metabolisms of the dominant phototroph and heterotroph in the oceans.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 8","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70119","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144782658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hydrogen Oxidation Benefits Alphaproteobacterial Methanotrophs Under Severe Methane Limitation 在严重的甲烷限制下，氢氧化有利于甲变形杆菌的甲烷氧化

IF 4 2区生物学

Environmental microbiology Pub Date : 2025-08-03 DOI: 10.1111/1462-2920.70163

Ida F. Peterse, Arjan Pol, Geert Cremers, Tom Berben, Theo A. van Alen, Huub J. M. Op den Camp, Annelies J. Veraart, Sebastian Lücker

{"title":"Hydrogen Oxidation Benefits Alphaproteobacterial Methanotrophs Under Severe Methane Limitation","authors":"Ida F. Peterse, Arjan Pol, Geert Cremers, Tom Berben, Theo A. van Alen, Huub J. M. Op den Camp, Annelies J. Veraart, Sebastian Lücker","doi":"10.1111/1462-2920.70163","DOIUrl":"10.1111/1462-2920.70163","url":null,"abstract":"Hydrogen (H2) and methane (CH4) are produced in the anoxic layers of wetlands and sediments. In the overlaying oxygenated surface layers, these gases become available for oxidation by aerobic hydrogenotrophic and methanotrophic microorganisms. While H2 oxidation by verrucomicrobial methane-oxidising bacteria (MOB) is extensively studied, less is known about this metabolism in MOB from the class Alphaproteobacteria, which frequently inhabit wetlands. We show that Methylocystis bryophila H2sT, Methylocapsa aurea KYGT, and “Methylosinus acidophilus” 29 encode diverse hydrogenases, instantly oxidise H2 when cultivated under CH4-limited and low-oxygen conditions, under which hydrogenase transcription is upregulated compared to CH4-replete conditions. H2 exposure accelerated the maximum H2 oxidation rates but caused no upregulation of hydrogenases. Furthermore, while CH4 oxidation activity was affected by substrate-limited growth conditions, H2 oxidation rates remained unaffected, and H2 supply to CH4-limited chemostats caused increased biomass yield. Moreover, CH4 oxidation was severely inhibited by sulfide (H2S), while H2 and methanol oxidation rates were only moderately affected. In summary, the ability to conserve energy from H2 oxidation increases resilience and enhances growth of alphaproteobacterial methanotrophs in CH4-limited environments, which revises the ecological role of these MOB in ecosystems with naturally fluctuating CH4 and H2 concentrations.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 8","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70163","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144767597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Contact- and Water-Mediated Interactions With an Allelopathic Macroalga Drive Distinct Coral Microbiome and Metabolome 接触和水介导的大藻化感作用驱动不同的珊瑚微生物组和代谢组

IF 4 2区生物学

Environmental microbiology Pub Date : 2025-07-31 DOI: 10.1111/1462-2920.70160

Chloé Pozas-Schacre, Hugo Bischoff, Camille Vizon, Delphine Raviglione, Camille Clerissi, Isabelle Bonnard, Maggy M. Nugues

{"title":"Contact- and Water-Mediated Interactions With an Allelopathic Macroalga Drive Distinct Coral Microbiome and Metabolome","authors":"Chloé Pozas-Schacre, Hugo Bischoff, Camille Vizon, Delphine Raviglione, Camille Clerissi, Isabelle Bonnard, Maggy M. Nugues","doi":"10.1111/1462-2920.70160","DOIUrl":"10.1111/1462-2920.70160","url":null,"abstract":"Macroalgal proliferation constitutes a major threat to coral reef resilience. Macroalgae can affect corals by altering their microbiome and metabolome. However, our understanding of the spatial scale of these effects and the influence of environmental factors is limited. We conducted a manipulative field experiment to investigate how interaction types (direct contact and close proximity) with the allelopathic macroalga Dictyota bartayresiana and prevailing water current influence the microbiome and metabolome of the coral Pocillopora acuta and its near-surface seawater. Coral tissue damage was spatially constrained to the algal contact zone. Direct contact caused significant increases in harmful bacteria at the expense of beneficial ones in side coral fragments. Non-significant changes were observed within the microbiome of apex fragments, suggesting a resistance of the coral holobiont to colony-wide microbial colonisation. The coral metabolome responded to both algal contact and proximity. We detected several compounds potentially relevant for oxidative stress mitigation and coral defence. This metabolomic response was similar between apex and side fragments, supportive of a colony-wide metabolomic response. In the near-surface coral seawater, only a microbial response to algal contact was detected. We conclude that coral holobionts are capable of colony-wide metabolomic responses to maintain homeostasis against macroalgal competitors.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 8","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70160","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144740543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In Situ Metabolic Rates of Alkane-Degrading Sulphate-Reducing Bacteria in Hydrocarbon Seep Sediments Revealed by Combining CARD-FISH, NanoSIMS, and Mathematical Modelling 结合CARD-FISH， NanoSIMS和数学模型揭示烃渗漏沉积物中烷烃降解硫酸盐还原菌的原位代谢率

IF 4 2区生物学

Environmental microbiology Pub Date : 2025-07-29 DOI: 10.1111/1462-2920.70151

Sara Kleindienst, Lubos Polerecky, Rudolf Amann, Florin Musat, Katrin Knittel

{"title":"In Situ Metabolic Rates of Alkane-Degrading Sulphate-Reducing Bacteria in Hydrocarbon Seep Sediments Revealed by Combining CARD-FISH, NanoSIMS, and Mathematical Modelling","authors":"Sara Kleindienst, Lubos Polerecky, Rudolf Amann, Florin Musat, Katrin Knittel","doi":"10.1111/1462-2920.70151","DOIUrl":"10.1111/1462-2920.70151","url":null,"abstract":"Marine hydrocarbon seeps are hotspots for sulphate reduction coupled to hydrocarbon oxidation. In situ metabolic rates of sulphate-reducing bacteria (SRB) degrading hydrocarbons other than methane, however, remain poorly understood. Here, we assessed the environmental role of Desulfosarcinaceae clades SCA1, SCA2 for degradation of n-butane and clade LCA2 for n-dodecane. Quantification by CARD-FISH showed that SCA1 constituted up to 31%, SCA2 up to 9%, and LCA2 up to 6% of cells from the recently re-classified class Deltaproteobacteria across diverse hydrocarbon seeps. Cell-specific oxidation rates estimated by stable-isotope probing combined with NanoSIMS and modelling were ~0.73 and ~2.11 fmol butane cell−1 d−1 for SCA1 and SCA2, respectively, and ~0.023 fmol dodecane cell−1 d−1 for LCA2 in sediments from Amon Mud Volcano and Guaymas Basin sediments. Cellular carbon assimilation, dissolved inorganic carbon production, and sulphate reduction rates indicated that butane-degrading SRB have higher metabolic activity than those utilising dodecane. Estimates based on in situ cell abundances, biovolumes, and cellular activities suggest that at certain seeps, clades SCA1, SCA2 and LCA2 account for nearly all sulphate reduction not driven by methane oxidation. These findings highlight the important role of alkane-degrading SRB in influencing marine carbon and sulphur cycles, particularly at seeps emitting higher hydrocarbons.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 8","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70151","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144716960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determinants of Cyanobacteria and Algae Diversity in Natural Freshwater Micro-Ecosystems 自然淡水微生态系统中蓝藻多样性的决定因素

IF 4 2区生物学

Environmental microbiology Pub Date : 2025-07-29 DOI: 10.1111/1462-2920.70157

Yasmin Rodrigues de Souza, Beatriz Melissa Campos, Fernando Miranda Lansac-toha, Annika Busse, Jana S. Petermann, Gustavo Quevedo Romero, Pablo Augusto Poleto Antiqueira, Luzia Cleide Rodrigues

{"title":"Determinants of Cyanobacteria and Algae Diversity in Natural Freshwater Micro-Ecosystems","authors":"Yasmin Rodrigues de Souza, Beatriz Melissa Campos, Fernando Miranda Lansac-toha, Annika Busse, Jana S. Petermann, Gustavo Quevedo Romero, Pablo Augusto Poleto Antiqueira, Luzia Cleide Rodrigues","doi":"10.1111/1462-2920.70157","DOIUrl":"10.1111/1462-2920.70157","url":null,"abstract":"In this context, estimating the contributions of single sites to overall beta diversity (LCBD—Local Contribution to Beta Diversity, i.e., indicator of site's ecological uniqueness) or partitioning overall beta diversity into contributions of individual species (SCBD—Species Contribution to Beta Diversity, i.e., degree of variation of individual species across the study area) has proven to be a good approach to improve the knowledge of drivers of beta diversity. The number of studies on beta diversity in hyperdiverse environments, such as the Neotropics, is still scarce. We explored the contributions of each site and species to the overall cyanobacteria and algae beta diversity of 77 natural freshwater micro-ecosystems (i.e., tank bromeliads) of a neotropical ecosystem. We observed that LCBD was negatively related to Shannon diversity, turbidity and luminosity (% canopy cover). The negative relationship between LCBD and Shannon diversity indicates that micro ecosystems with less diversity reflect unique characteristics, and LCBD values can predict these environments. In our study, high LCBD values indicated environments in need of restoration, that is, poor in species richness and with greater turbidity and luminosity, showing that most bromeliad tanks presented high species diversity and low turbidity and luminosity.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 8","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70157","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144716961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring Energy Conservation in Sulphate-Dependent Anaerobic Methane-Oxidising Consortia Through Metabolic Modelling 通过代谢模型探索硫酸盐依赖性厌氧甲烷氧化联合体的能量节约

IF 4 2区生物学

Environmental microbiology Pub Date : 2025-07-24 DOI: 10.1111/1462-2920.70156

Gordon Bowman, Zena Jensvold, Qusheng Jin

{"title":"Exploring Energy Conservation in Sulphate-Dependent Anaerobic Methane-Oxidising Consortia Through Metabolic Modelling","authors":"Gordon Bowman, Zena Jensvold, Qusheng Jin","doi":"10.1111/1462-2920.70156","DOIUrl":"10.1111/1462-2920.70156","url":null,"abstract":"Anaerobic oxidation of methane (AOM) coupled with sulphate reduction (SR) is a crucial microbial process that mitigates methane emissions, a major contributor to climate change. However, the bioenergetics underlying this process remains poorly understood. Here, we present a metabolic model to quantify energy fluxes and conservation in AOM consortia by integrating enzyme-level thermodynamics and kinetics. Unlike previous models that impose artificial constraints on energy conservation kinetics and efficiency, our approach mechanistically predicts ATP yields and energy efficiencies. We show that both anaerobic methanotrophic archaea (ANME) and sulphate-reducing bacteria (SRB) invest energy in substrate activation, synthesising ATP with comparable yields (0.23–0.24 mol ATP per mol methane or sulphate), while achieving remarkable thermodynamic efficiency (~60%). However, ANME exhibit a higher return on investment (ROI, 18%) than SRB (11%) due to more efficient substrate activation. These findings highlight fundamental bioenergetic constraints governing methane oxidation and SR in anoxic environments, enhancing our understanding of how microbial processes regulate methane fluxes in natural ecosystems. By providing a quantitative framework for microbial energy conservation, our study advances biogeochemical modelling and informs strategies for methane mitigation in marine sediments and other anaerobic environments critical to climate regulation.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 7","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70156","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144695885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Is Everything Everywhere? Dispersal Limitation Impacts Methanotroph Community Functioning 万物无处不在吗？扩散限制对甲烷化菌群落功能的影响

IF 4 2区生物学

Environmental microbiology Pub Date : 2025-07-24 DOI: 10.1111/1462-2920.70158

Jie Fang, Yongcui Deng, Zihao Liu, Jonathan M. Adams

{"title":"Is Everything Everywhere? Dispersal Limitation Impacts Methanotroph Community Functioning","authors":"Jie Fang, Yongcui Deng, Zihao Liu, Jonathan M. Adams","doi":"10.1111/1462-2920.70158","DOIUrl":"10.1111/1462-2920.70158","url":null,"abstract":"<div>\u0000 \u0000 The significance of dispersal limitation in microbial ecology and biogeography remains debated. We aimed to clarify the role of dispersal limitation in the adaptation of methanotroph communities to salt-stress, essentially testing the ‘everything is everywhere’ hypothesis in functional terms. Riparian sediments along the Yangtze River and lakeshore sediments at varying geographical distances inland from the river were collected. Microcosms were incubated with ~5% CH4 under three conditions: 50 g/L salinity, 50 g/L salinity plus a methanotroph community inoculum, and a control. We observed a significant delay in methane oxidation at increased salinity, but salt-tolerant methanotrophic activity persisted in riparian sediments. Using DNA-SIP, we identified halotolerant Methylobacter-taxa that possibly dispersed from the saline estuary. By contrast, in lakes/ponds inland away from the Yangtze, progressively fewer samples oxidised methane under high salinity without inoculation, until at 130 km distance, no samples could adapt. Methanotrophy was restored in every case by inoculation with propagules from the saline Yangtze Delta, confirming the impact of dispersal limitation of halotolerant Methylobacter-propagules in constraining ecosystem functional adaptation. By focusing on ecosystem functions rather than just taxonomic communities, this study uniquely tests a key paradigm in microbial ecology, suggesting that broad-scale microbial dispersal limitation can constrain ecosystem adaptation.\u0000 </div>","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 7","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144695889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Eutrophication and Warming Drive Algal Community Shifts in Synchronised Time Series of Experimental Lakes 富营养化和变暖驱动实验湖藻类群落同步时间序列变化

IF 4 2区生物学

Environmental microbiology Pub Date : 2025-07-24 DOI: 10.1111/1462-2920.70159

Rebecca E. Garner, Zofia E. Taranu, Scott N. Higgins, Michael J. Paterson, Irene Gregory-Eaves, David A. Walsh

{"title":"Eutrophication and Warming Drive Algal Community Shifts in Synchronised Time Series of Experimental Lakes","authors":"Rebecca E. Garner, Zofia E. Taranu, Scott N. Higgins, Michael J. Paterson, Irene Gregory-Eaves, David A. Walsh","doi":"10.1111/1462-2920.70159","DOIUrl":"10.1111/1462-2920.70159","url":null,"abstract":"Lake ecosystems are increasingly impacted by eutrophication and climate change. Whole-lake experiments have provided ecosystem-scale insights into the effects of freshwater stressors, yet these are constrained to the duration of monitoring programmes. Here, we leveraged multidecadal monitoring records and century-scale paleogenetic reconstructions for experimentally fertilised and unmanipulated lakes in the IISD Experimental Lakes Area of northwestern Ontario, Canada, to evaluate the responses of algal communities to nutrient and air temperature variation. We first validated the paleogenetic analysis of sediment DNA by demonstrating the synchrony of algal community changes with monitoring records. Algal communities underwent significant compositional shifts across experimental nutrient loading regimes and climate periods, with baseline assemblages informed by paleogenetics. Nonlinear regression modelling of algal community change in monitoring and paleogenetic time series showed the expected response that nutrients were strong drivers in fertilised lakes. Paleogenetic records reflected the century-scale impacts of climate warming and its combined effects with eutrophication, previously underestimated by monitoring. The synergy between eutrophication and warming points to eutrophic priming of the food web to respond to rising temperatures. Overall, the paleogenetic integration of algal diversity across habitats and seasons enables the detection of slow-acting climate change on lake ecosystems increasingly altered by nutrient pollution.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 7","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70159","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144695886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cyanophage Infections in a Sponge Intracellular Cyanobacterial Symbiont 海绵细胞内蓝藻共生体中的噬藻感染

IF 4 2区生物学

Environmental microbiology Pub Date : 2025-07-22 DOI: 10.1111/1462-2920.70155

Tzipora Peretz, Esther Cattan-Tsaushu, Chiara Conti, Benyamin Rosental, Laura Steindler, Sarit Avrani

{"title":"Cyanophage Infections in a Sponge Intracellular Cyanobacterial Symbiont","authors":"Tzipora Peretz, Esther Cattan-Tsaushu, Chiara Conti, Benyamin Rosental, Laura Steindler, Sarit Avrani","doi":"10.1111/1462-2920.70155","DOIUrl":"10.1111/1462-2920.70155","url":null,"abstract":"Sponges are sessile animals that play crucial roles in marine ecosystems by facilitating nutrient cycling, enhancing biodiversity, and structuring benthic habitats. Microbial symbionts, including cyanobacteria, are vital to sponges, aiding in nutrient cycling, metabolism, and defence. However, due to the sponge's ability to concentrate phages from seawater, extracellular sponge symbionts are particularly vulnerable to phage infection. By contrast, little is known about the susceptibility of intracellular sponge symbionts to phage predation. Here, we present evidence that Candidatus Synechococcus feldmannii, a facultative, horizontally transmitted cyanobacterial endosymbiont of the sponge Petrosia ficiformis, is susceptible to cyanophages. We analysed four Ca. S. feldmannii genomes and found evidence for phage interactions in two, including CRISPR spacers matching sipho- and T4-like cyanophages. One genome harboured a prophage region resembling freshwater cyanobacterial prophages, featuring conserved regions associated with Type VI secretion systems, similar to Wolbachia endosymbionts prophages. Additionally, we developed a method for isolating cyanophages directly from purified sponge bacteriocytes (specialised sponge cells harbouring symbionts) and identified nine T4-like cyanophages with less than 60% similarity to known relatives. Collectively, our findings indicate that Ca. S. feldmannii is susceptible to cyanophages and suggest potential functional parallels between phages infecting endosymbionts across different animal hosts.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 7","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70155","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144681519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Distribution of Surface-Layer Prokaryotes in the Western Arctic Ocean: Responses to Pacific Water Inflow and Sea Ice Melting 北冰洋西部表层原核生物的分布：对太平洋水流入和海冰融化的响应

IF 4 2区生物学

Environmental microbiology Pub Date : 2025-07-21 DOI: 10.1111/1462-2920.70154

Puthiya Veettil Vipindas, Siddarthan Venkatachalam, Thajudeen Jabir, Eun Jin Yang, Kyoung-Ho Cho, Jinyoung Jung, Youngju Lee, Jong-Kuk Moon, Anand Jain

{"title":"Distribution of Surface-Layer Prokaryotes in the Western Arctic Ocean: Responses to Pacific Water Inflow and Sea Ice Melting","authors":"Puthiya Veettil Vipindas, Siddarthan Venkatachalam, Thajudeen Jabir, Eun Jin Yang, Kyoung-Ho Cho, Jinyoung Jung, Youngju Lee, Jong-Kuk Moon, Anand Jain","doi":"10.1111/1462-2920.70154","DOIUrl":"10.1111/1462-2920.70154","url":null,"abstract":"<div>\u0000 \u0000 Here, we evaluated how microbial community composition and functions vary along the path of Pacific water inflow, starting from the Bering Sea via the Chukchi Sea to the central Arctic Ocean. Our findings reveal that the inflow of Pacific water and sea ice melt significantly influence the environmental settings of the western Arctic Ocean, resulting in distinct prokaryotic communities with varied distribution patterns between the open Chukchi Sea and the Ice-covered central Arctic Ocean. The heterotrophic populations reliant on phytoplankton predominated in the Bering Sea and Southern Chukchi Sea, while in the Central Arctic Ocean, chemoautotrophic bacteria and archaea contributed equally with heterotrophic populations adapted to oligotrophic conditions. Although no specific functional genes were universally enriched across the metagenome libraries of prokaryotic communities, the relative abundance of functional genes varied among oceanic sectors. The assembly processes of prokaryotic communities in the western Arctic Ocean were found to be influenced by both deterministic and stochastic factors, with deterministic processes playing a more significant role. Thus, the ongoing increases in Pacific inflow and sea ice melt could lead to the displacement of native chemoautotrophic and oligotrophic populations in the Arctic Ocean by fast-growing heterotrophic populations better adapted to elevated nutrient concentrations and temperatures.\u0000 </div>","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 7","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144678136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0