Warming Seawater Temperature and Nutrient Depletion Alters Microbial Community Composition on a Foundational Canopy Kelp Species

Abstract

Warming seawater temperatures and low dissolved inorganic nitrogen (DIN) levels are environmental stressors that affect the health and abundance of marine macroalgae and their microbiomes. Nereocystis luetkeana, a canopy-forming species of brown algae that forms critical habitat along the Pacific coast, has declined in regions impacted by these synergistic stressors. Little is known about how these environmental factors affect the microbiome of N. luetkeana, which could affect nutrient availability, vitamin production, and stress response for the host. We experimentally tested the interactive effects of three seawater temperatures (13°C, 16°C, 21°C) crossed with abundant and replete DIN levels on the diversity and composition of blade-associated microbiomes from two spatially separated kelp host populations. We hypothesised that kelp microbiomes exposed to high temperatures and low DIN would experience the lowest diversity. Contrary to our hypothesis, the highest temperature treatment resulted in the largest increase in microbial diversity, and microbiomes in all temperature treatments experienced a decrease in previously dominant taxa. Temperature had a larger effect than DIN on the kelp microbiome in all cases. The disruption to the kelp microbiome across all temperatures, especially at the highest temperature, suggests that the effects of warming on N. luetkeana extend to the microbiome.