Environmental microbiology最新文献_第5页

Volatile Organic Compounds of Diverse Origins and Their Changes Associated With Cultivar Decay in a Fungus-Farming Termite

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-02-05 DOI: 10.1111/1462-2920.70049

Nanna Hjort Vidkjær, Suzanne Schmidt, Cleo Lisa Davie-Martin, Kolotchèlèma Simon Silué, N'golo Abdoulaye Koné, Riikka Rinnan, Michael Poulsen

{"title":"Volatile Organic Compounds of Diverse Origins and Their Changes Associated With Cultivar Decay in a Fungus-Farming Termite","authors":"Nanna Hjort Vidkjær, Suzanne Schmidt, Cleo Lisa Davie-Martin, Kolotchèlèma Simon Silué, N'golo Abdoulaye Koné, Riikka Rinnan, Michael Poulsen","doi":"10.1111/1462-2920.70049","DOIUrl":"10.1111/1462-2920.70049","url":null,"abstract":"Fungus-farming termites cultivate a Termitomyces fungus monoculture in enclosed gardens (combs) free of other fungi, except during colony declines, where Pseudoxylaria spp. stowaway fungi appear and take over combs. Here, we determined Volatile Organic Compounds (VOCs) of healthy Macrotermes bellicosus nests in nature and VOC changes associated with comb decay during Pseudoxylaria takeover. We identified 443 VOCs and unique volatilomes across samples and nest volatilomes that were mainly composed of fungus comb VOCs with termite contributions. Few comb VOCs were linked to chemical changes during decay, but longipinocarvone and longiverbenone were only emitted during comb decay. These terpenes may be involved in Termitomyces defence against antagonistic fungi or in fungus-termite signalling of comb state. Both comb and Pseudoxylaria biomass volatilomes contained many VOCs with antimicrobial activity that may serve in maintaining healthy Termitomyces monocultures or aid in the antagonistic takeover by Pseudoxylaria during colony decline. We further observed a series of oxylipins with known functions in the regulation of fungus germination, growth, and secondary metabolite production. Our volatilome map of the fungus-farming termite symbiosis provides new insights into the chemistry regulating complex interactions and serves as a valuable guide for future work on the roles of VOCs in symbioses.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 2","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70049","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143192106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic Transfer in Action: Uncovering DNA Flow in an Extremophilic Microbial Community

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-02-03 DOI: 10.1111/1462-2920.70048

Julia Van Etten, Timothy G. Stephens, Debashish Bhattacharya

{"title":"Genetic Transfer in Action: Uncovering DNA Flow in an Extremophilic Microbial Community","authors":"Julia Van Etten, Timothy G. Stephens, Debashish Bhattacharya","doi":"10.1111/1462-2920.70048","DOIUrl":"10.1111/1462-2920.70048","url":null,"abstract":"Horizontal genetic transfer (HGT) is a significant driver of genomic novelty in all domains of life. HGT has been investigated in many studies however, the focus has been on conspicuous protein-coding DNA transfers that often prove to be adaptive in recipient organisms and are therefore fixed longer-term in lineages. These results comprise a subclass of HGTs and do not represent exhaustive (coding and non-coding) DNA transfer and its impact on ecology. Uncovering exhaustive HGT can provide key insights into the connectivity of genomes in communities and how these transfers may occur. In this study, we use the term frequency-inverse document frequency (TF-IDF) technique, that has been used successfully to mine DNA transfers within real and simulated high-quality prokaryote genomes, to search for exhaustive HGTs within an extremophilic microbial community. We establish a pipeline for validating transfers identified using this approach. We find that most DNA transfers are within-domain and involve non-coding DNA. A relatively high proportion of the predicted protein-coding HGTs appear to encode transposase activity, restriction-modification system components, and biofilm formation functions. Our study demonstrates the utility of the TF-IDF approach for HGT detection and provides insights into the mechanisms of recent DNA transfer.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 2","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70048","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143083566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Temporal and Spatial Dynamics of Synechococcus Clade II and Other Microbes in the Eutrophic Subtropical San Diego Bay

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-02-03 DOI: 10.1111/1462-2920.70043

Katie J. Harding, Maitreyi Nagarkar, Maggie Wang, Kailey Ramsing, Niv Anidjar, Sarah Giddings, Bianca Brahamsha, Brian Palenik

{"title":"Temporal and Spatial Dynamics of Synechococcus Clade II and Other Microbes in the Eutrophic Subtropical San Diego Bay","authors":"Katie J. Harding, Maitreyi Nagarkar, Maggie Wang, Kailey Ramsing, Niv Anidjar, Sarah Giddings, Bianca Brahamsha, Brian Palenik","doi":"10.1111/1462-2920.70043","DOIUrl":"10.1111/1462-2920.70043","url":null,"abstract":"The diversity of the marine cyanobacterium Synechococcus can be broadly separated into clades, with clade II typically present in warm oligotrophic water, and clades I and IV found in cooler coastal water. We found amplicon sequence variants (ASVs) belonging to clade II in the nutrient-replete waters of San Diego Bay (SDB). Using the 16S rRNA gene, 18S rRNA gene and internal transcribed spacer region sequencing, we analysed multiple locations in SDB monthly for over a year, with additional samples dating back to 2015. Synechococcus community composition differed from the nearby coast into SDB in terms of dominant clade and ASVs. Specific clade II ASVs became relatively more abundant towards the back of the bay and showed seasonality, with higher relative abundance in the warm months. Select ASVs group phylogenetically and show similar seasonal and spatial distribution patterns, indicating these ASVs have adapted to SDB. Isolates matching clade II ASVs from SDB show pigment composition that is better adapted to the green light available in SDB, further supporting our findings. Other microbial taxa also show SDB enrichment, providing evidence that SDB is a chemostat-like environment where circulation, temperature, light and other environmental conditions create a zone for microbial evolution and diversification.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 2","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70043","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143083567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacterial Diversity of Marine Biofilm Communities in Terra Nova Bay (Antarctica) by Culture-Dependent and -Independent Approaches

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-02-02 DOI: 10.1111/1462-2920.70045

Bisaccia Melissa, Binda Elisa, Caruso Gabriella, Azzaro Maurizio, Dell' Acqua Ombretta, Di Cesare Andrea, Ester Maria Eckert, Marinelli Flavia

{"title":"Bacterial Diversity of Marine Biofilm Communities in Terra Nova Bay (Antarctica) by Culture-Dependent and -Independent Approaches","authors":"Bisaccia Melissa, Binda Elisa, Caruso Gabriella, Azzaro Maurizio, Dell' Acqua Ombretta, Di Cesare Andrea, Ester Maria Eckert, Marinelli Flavia","doi":"10.1111/1462-2920.70045","DOIUrl":"10.1111/1462-2920.70045","url":null,"abstract":"Applying both culture-independent and -dependent approaches, bacterial diversity of marine biofilm communities colonising polyvinyl chloride panels submerged in Terra Nova Bay (Ross Sea, Antarctica) was investigated. Panels were deployed in two sites subjected to a different degree of anthropogenic impact (Road Bay [RB] impacted site and Punta Stocchino [PTS] control site). Biofilm samples were collected after 3 or 12 months to evaluate both short- and long-term microbial colonisation. Taxonomic composition of the microbial community was studied by 16S rRNA gene amplicon sequencing. Proteobacteria was the predominant phylum, followed by Bacteroidetes, Actinobacteria, Verrucomicrobia and Firmicutes. Impacted RB biofilms were found to contain a relevant fraction of potentially pathogenic bacterial genera, accounting for 27.49% of the whole community. A total of 86 psychrotolerant bacterial strains were isolated from the biofilm samples using culture-dependent techniques designed to enrich in Actinobacteria. These strains were assigned to three different phyla: Actinobacteria (54.65%), Firmicutes (32.56%) and Proteobacteria (12.79%). 2.73% of genera identified by metabarcoding were recovered also through cultivation, while 11 additional genera were uniquely yielded by cultivation. Functional screening of the isolates revealed their hydrolytic and oxidative enzyme activity patterns, giving new insights into the metabolic and biotechnological potential of microbial biofilm communities in Terra Nova Bay seawater.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 2","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70045","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143077200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Archaeal Signalling Networks—New Insights Into the Structure and Function of Histidine Kinases and Response Regulators of the Methanogenic Archaeon Methanosarcina acetivorans

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-01-31 DOI: 10.1111/1462-2920.70047

Nora F. K. Georgiev, Anne L. Andersson, Zoe Ruppe, Loriana Kattwinkel, Nicole Frankenberg-Dinkel

{"title":"Archaeal Signalling Networks—New Insights Into the Structure and Function of Histidine Kinases and Response Regulators of the Methanogenic Archaeon Methanosarcina acetivorans","authors":"Nora F. K. Georgiev, Anne L. Andersson, Zoe Ruppe, Loriana Kattwinkel, Nicole Frankenberg-Dinkel","doi":"10.1111/1462-2920.70047","DOIUrl":"10.1111/1462-2920.70047","url":null,"abstract":"The methanogenic archaeon Methanosarcina acetivorans has one of the largest known archaeal genomes. With 53 histidine kinases (HK), it also has the largest set of signal transduction systems. To gain insight into the hitherto not very well understood signal transduction in Archaea and M. acetivorans in particular, we have categorised the predicted HK into four types based on their H-box using an in silico analysis. Representatives of three types were recombinantly produced in Escherichia coli and purified by affinity chromatography. All investigated kinases showed ATP binding and hydrolysis. The MA_type 2 kinase, which lacks the classical H-box, showed no autokinase activity. Furthermore, we could show that M. acetivorans possesses an above-average number of response regulators (RR), consisting of only a REC domain (REC-only). Using the hybrid kinase MA4377 as an example we show that both intra-and intermolecular transphosphorylation to REC domains occur. These experiments are furthermore indicative of complex phosphorelay systems in M. acetivorans and suggest that REC-only proteins act as a central hub in signal transduction in M. acetivorans.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 2","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11784639/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143064685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adaptive Laboratory Evolution Uncovers Potential Role of a DNA Helicase Mutation in Torulaspora delbrueckii Increased Sulphite Resistance

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-01-31 DOI: 10.1111/1462-2920.70038

Carolina Osório, Ticiana Fernandes, Teresa Rito, Pedro Soares, Ricardo Franco-Duarte, Maria João Sousa

{"title":"Adaptive Laboratory Evolution Uncovers Potential Role of a DNA Helicase Mutation in Torulaspora delbrueckii Increased Sulphite Resistance","authors":"Carolina Osório, Ticiana Fernandes, Teresa Rito, Pedro Soares, Ricardo Franco-Duarte, Maria João Sousa","doi":"10.1111/1462-2920.70038","DOIUrl":"10.1111/1462-2920.70038","url":null,"abstract":"<div>\u0000 \u0000 Wine industry has faced pressure to innovate its products. Saccharomyces cerevisiae has been the traditional yeast for producing alcoholic beverages, but interest has shifted from the conventional S. cerevisiae to non-Saccharomyces yeasts for their biotechnological potential. Among these, Torulaspora delbrueckii is particularly notable for its ability to enrich wine with novel flavours. During winemaking, sulphites are added to suppress spoilage microorganisms, making sulphite tolerance a valuable characteristic of wine yeasts. Adaptive laboratory evolution in liquid and solid media improved sulphite resistance in two T. delbrueckii strains, achieving, in the best case, a fourfold increase from 0.50 to 2.00 mM of sodium metabisulphite, highlighting the potential of these evolve strains for winemaking applications. Genomic analysis revealed SNPs/InDels in all the strains, including a novel unique missense mutation common to the four evolved isolates, but absent from the parental strains, located in chromosome VIII (protein TDEL0H03170, homologue of S. cerevisiae MPH1). These genes code for a protein catalogued as an ATP-dependent DNA helicase, known for its role in maintaining genome stability by participating in DNA repair pathways. We propose that this valine-to-serine mutation, common to all the evolved isolates, helps the evolved strains repair sulphite-induced DNA damage more effectively.\u0000 </div>","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 2","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143064516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbiome Stability Is Linked to Acropora Coral Thermotolerance in Northwestern Philippines

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-01-31 DOI: 10.1111/1462-2920.70041

Jake Ivan P. Baquiran, John Bennedick Quijano, Madeleine J. H. van Oppen, Patrick C. Cabaitan, Peter L. Harrison, Cecilia Conaco

{"title":"Microbiome Stability Is Linked to Acropora Coral Thermotolerance in Northwestern Philippines","authors":"Jake Ivan P. Baquiran, John Bennedick Quijano, Madeleine J. H. van Oppen, Patrick C. Cabaitan, Peter L. Harrison, Cecilia Conaco","doi":"10.1111/1462-2920.70041","DOIUrl":"10.1111/1462-2920.70041","url":null,"abstract":"<div>\u0000 \u0000 Corals associate with a diverse community of prokaryotic symbionts that provide nutrition, antioxidants and other protective compounds to their host. However, the influence of microbes on coral thermotolerance remains understudied. Here, we examined the prokaryotic microbial communities associated with colonies of Acropora cf. tenuis that exhibit high or low thermotolerance upon exposure to 33°C (heated) relative to 29°C (control). Using 16S rRNA sequencing, we show that the microbial community structure of all A. cf. tenuis colonies was similar to each other at control temperature. Thermotolerant colonies, however, had relatively greater abundance of Endozoicomonas, Arcobacter, Bifidobacterium and Lactobacillus. At elevated temperature, only thermosensitive colonies showed a distinct shift in their microbiome, with an increase in Flavobacteriales, Rhodobacteraceae and Vibrio, accompanying a marked bleaching response. Functional prediction indicated that prokaryotic communities associated with thermotolerant corals were enriched for genes related to metabolism, while microbiomes of thermosensitive colonies were enriched for cell motility and antibiotic compound synthesis. These differences may contribute to the variable performance of thermotolerant and thermosensitive corals under thermal stress. Identification of microbial taxa correlated with thermotolerance provides insights into beneficial bacterial groups that could be used for microbiome engineering to support reef health in a changing climate.\u0000 </div>","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 2","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143064703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Water Quality and Land Use Shape Bacterial Communities Across 621 Canadian Lakes

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-01-27 DOI: 10.1111/1462-2920.70037

Vera E. Onana, Beatrix E. Beisner, David A. Walsh

{"title":"Water Quality and Land Use Shape Bacterial Communities Across 621 Canadian Lakes","authors":"Vera E. Onana, Beatrix E. Beisner, David A. Walsh","doi":"10.1111/1462-2920.70037","DOIUrl":"10.1111/1462-2920.70037","url":null,"abstract":"<div>\u0000 \u0000 Human activities such as agriculture and urban development are linked to water quality degradation. Canada represents a large and heterogeneous landscape of freshwater lakes, where variations in climate, geography and geology interact with land cover alteration to influence water quality differently across regions. In this study, we investigated the influence of water quality and land use on bacterial communities across 12 ecozones. At the pan-Canadian scale, total phosphorus (TP) was the most significant water quality variable influencing community structure, and the most pronounced shift was observed at 110 μg/L of TP, corresponding to the transition from eutrophic to hypereutrophic conditions. At the regional scale, water quality significantly explained bacterial community structure in all ecozones. In terms of land use effect, at the pan-Canadian scale, agriculture and, to a lesser extent, urbanisation were significant land use variables influencing community structure. Regionally, in ecozones characterised by extensive agriculture, this land cover variable was consistently significant in explaining community structure. Likewise, in extensively urbanised ecozones, urbanisation was consistently significant in explaining community structure. Overall, these results demonstrate that bacterial richness and community structure are influenced by water quality and shaped by agriculture and urban development in different ways.\u0000 </div>","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143044763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ring A Cleaving Beta-Diketone Hydrolase Is a Key Enzyme of Steroid Degradation in Anaerobic Bacteria

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-01-27 DOI: 10.1111/1462-2920.70034

Christian Jacoby, Lina Peller, Jana Wenzler, Monika Luttermann, Wolfgang Seiche, Bernhard Breit, Matthias Boll

{"title":"Ring A Cleaving Beta-Diketone Hydrolase Is a Key Enzyme of Steroid Degradation in Anaerobic Bacteria","authors":"Christian Jacoby, Lina Peller, Jana Wenzler, Monika Luttermann, Wolfgang Seiche, Bernhard Breit, Matthias Boll","doi":"10.1111/1462-2920.70034","DOIUrl":"10.1111/1462-2920.70034","url":null,"abstract":"Bacterial degradation of ubiquitous and persistent steroids such as steroid hormones is important for their removal from the environment. Initial studies of steroid degradation in anaerobic bacteria suggested that ring-cleaving hydrolases are involved in oxygen-independent sterane skeleton degradation. However, the enzymes involved in ring A cleavage of the common intermediate androsta-1,4-diene-3,17-dione have remained unknown. Here, we enriched a ring A hydrolase from cholesterol/nitrate grown Sterolibacterium denitrificans and from Escherichia coli after heterologous expression of its gene. This enzyme specifically cleaves the cyclic 1,3-diketone of the central degradation intermediate, androsta-1,3,17-trione to 1,17-dioxo-2,3-seco-androstan-3-oate (DSAO), a hallmark reaction of anaerobic steroid degradation. The highly conserved ring A hydrolase was identified in all known and many previously unknown steroid-degrading proteobacteria. Using enriched enzymes, we enzymatically produced DSAO from the chemically synthesised androsta-1-en-3,17-dione precursor, allowing the identification of subsequent metabolites involved in ring A degradation. The results obtained suggest the involvement of an additional hydrolase, an aldolase, and a β-oxidation-like cascade for complete ring A degradation to form the three-ring 5,10-seco-1,2,3,4-tetranorandrosta-5,17-dione. The results identified a key enzyme of anaerobic steroid degradation that may serve as a functional marker for monitoring steroid contaminant degradation at anoxic environmental sites.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70034","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143044201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characteristics of Phages and Their Interactions With Hosts in Anaerobic Reactors

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-01-27 DOI: 10.1111/1462-2920.70040

Yan Zeng, Xiaozhong Zhong, Yating Chen, Min Gou, Ke Yu, Yue-Qin Tang

{"title":"Characteristics of Phages and Their Interactions With Hosts in Anaerobic Reactors","authors":"Yan Zeng, Xiaozhong Zhong, Yating Chen, Min Gou, Ke Yu, Yue-Qin Tang","doi":"10.1111/1462-2920.70040","DOIUrl":"10.1111/1462-2920.70040","url":null,"abstract":"<div>\u0000 \u0000 Anaerobic digestion (AD) of organic wastes relies on the interaction and cooperation of various microorganisms. Phages are crucial components of the microbial community in AD systems, but their diversity and interactions with the prokaryotic populations are still inadequately comprehended. In this study, 2121 viral operational taxonomic units (vOTUs) were recovered from 12 anaerobic fatty acid–fed reactors. Notably, 63.1% of these vOTUs could not be assigned to any known family, revealing a substantial presence of uncharted phages specifically associated with AD environments. Over half of the vOTUs associated with hosts had the capability to infect multiple hosts, ranging from 2 to 49, with a prevalent tendency to infect 2–5 hosts. In silico predictions of phage-host linkages uncovered that only a small fraction of vOTUs were shared across different functional groups, including fermentative bacteria, syntrophic fatty acid–oxidising bacteria (SFOB) and methanogens. Phages linked to hosts in all three groups primarily consisted of generalists and temperate species, especially those linked to SFOB. Additionally, metabolic reconstruction identified auxiliary metabolic genes participating in fatty acid degradation, methanogenesis and energy conservation. The present study provides insights into phage characteristics and their in situ interactions with prokaryotic hosts, highlighting their ecological role in AD systems.\u0000 </div>","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143044200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0