Environmental microbiology最新文献_第5页

Intertidal Warming Causes Mortality and Disrupts the Microbiome of Oysters 潮间带变暖导致牡蛎死亡并破坏微生物群

IF 4 2区生物学

Environmental microbiology Pub Date : 2025-07-18 DOI: 10.1111/1462-2920.70152

Elliot Scanes, Nachshon Siboni, Maquel Brandimarti, Justin Seymour

{"title":"Intertidal Warming Causes Mortality and Disrupts the Microbiome of Oysters","authors":"Elliot Scanes, Nachshon Siboni, Maquel Brandimarti, Justin Seymour","doi":"10.1111/1462-2920.70152","DOIUrl":"10.1111/1462-2920.70152","url":null,"abstract":"Intertidal ecosystems are physically stressful habitats, with resident organisms often living close to their limits. These limits include the balance between host organisms and microbial partners; a balance that may be tipped by climate change. We simulated intertidal warming in the field by establishing populations of the Sydney rock oyster, Saccostrea glomerata, on black and white concrete tiles, resulting in differing thermal conditions. Tiles were placed on the intertidal shoreline among natural oyster populations. Oysters on black tiles were up to 3°C warmer than those on white tiles during low tide. We monitored the tiles for oyster survival and took gill and haemolymph samples from oysters for microbiological analysis using qPCR, 16S, and HSP60 rRNA sequencing. We found that after six days, levels of oyster mortality were 50% greater on the black tiles. Oysters on black tiles exhibited a significant shift in their microbiome, involving increases in putative pathogenic bacteria from the Vibrio genus, including the known oyster pathogen V. harveyi and the human pathogen V. parahaemolyticus. These findings demonstrate that relatively small increases in temperature within intertidal ecosystems can cause significant shifts in the microbiome and mortality among oyster populations, with putative links to bacterial pathogens.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 7","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70152","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144657602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Drivers of Marine Phytoplankton Diversity and Connectivity in the Galápagos Archipelago Spanning an ENSO Cycle 横跨ENSO循环的Galápagos群岛海洋浮游植物多样性和连通性的驱动因素

IF 4 2区生物学

Environmental microbiology Pub Date : 2025-07-18 DOI: 10.1111/1462-2920.70146

Prisca Lim, Harvey Seim, Oliva Torano, Erika Neave, Se Hyeon Jang, Zackary Johnson, Sara Haines, Scott Gifford, Natalie Cohen, Carly M. Moreno, Margarita Lankford, Cristina Vintimilla Palacios, Adrian Marchetti

{"title":"Drivers of Marine Phytoplankton Diversity and Connectivity in the Galápagos Archipelago Spanning an ENSO Cycle","authors":"Prisca Lim, Harvey Seim, Oliva Torano, Erika Neave, Se Hyeon Jang, Zackary Johnson, Sara Haines, Scott Gifford, Natalie Cohen, Carly M. Moreno, Margarita Lankford, Cristina Vintimilla Palacios, Adrian Marchetti","doi":"10.1111/1462-2920.70146","DOIUrl":"10.1111/1462-2920.70146","url":null,"abstract":"<div>\u0000 \u0000 The Galápagos Islands are a biodiversity hotspot, largely due to the Equatorial Undercurrent (EUC) which supplies nutrient-rich waters to the euphotic zone and supports enhanced levels of primary productivity performed by phytoplankton. Understanding phytoplankton responses to changing environmental conditions is crucial for regional conservation and management efforts. Research cruises conducted between 2014 and 2022, spanning a major El Niño event in 2015 and a La Niña event in 2022, observed varying oceanic conditions and diverse phytoplankton community composition. At most EUC-influenced stations, larger-sized phytoplankton groups (≥ 5 μm) were dominant while warmer, oligotrophic sites favoured smaller-sized phytoplankton groups (< 5 μm). Predictably, nutrient supply was suppressed during the El Niño event associated with the weakening of the EUC and deepening of the thermocline. Counterintuitively, nutrient levels were not significantly enhanced during the La Niña event likely because increased stratification between the mixed and deep water layers reduced entrainment, particularly at Eastern stations. Protist community composition was evaluated using 18S rRNA gene metabarcoding; the majority of detected OTUs were associated with upwelling conditions prevalent around the archipelago. Taxonomic variability reflected heterogeneous environmental conditions generated by the convergence of multiple ocean currents. These results highlight the dynamic interplay of physical and biological factors shaping primary productivity in the Galápagos marine ecosystem.\u0000 </div>","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 7","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144647231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacterial Dynamics in Newly Settled Acropora kenti: Insights From Inoculations With Individual Probiotic Candidates 新定居的肯氏Acropora的细菌动力学：从接种单个候选益生菌的见解

IF 4 2区生物学

Environmental microbiology Pub Date : 2025-07-16 DOI: 10.1111/1462-2920.70143

Callaway Thatcher, Katarina Damjanovic, Felicity Kuek, Patrick W. Laffy, David G. Bourne, Lone Høj

{"title":"Bacterial Dynamics in Newly Settled Acropora kenti: Insights From Inoculations With Individual Probiotic Candidates","authors":"Callaway Thatcher, Katarina Damjanovic, Felicity Kuek, Patrick W. Laffy, David G. Bourne, Lone Høj","doi":"10.1111/1462-2920.70143","DOIUrl":"10.1111/1462-2920.70143","url":null,"abstract":"Bacterial probiotics hold promise for enhancing coral health and stress resilience; however, their application to coral juveniles, which are critical for restorative aquaculture, remains underexplored. Here, we show that the microbiome of newly settled Acropora kenti is amenable to manipulation, with individual probiotic candidates inducing distinct microbiome responses that differed in their stability after the final inoculation. Three of the eight tested strains, Halomonas smyrnensis, Endozoicomonas acroporae and Roseivivax lentus, remained prevalent in the microbiome at least 5 days post-inoculation. Notably, aggregates of Endozoicomonas cells were observed exclusively in the corresponding treatment, marking the first report of coral-associated microbial aggregates (CAMAs) in early life stage acroporids and the first induced through bacterial manipulation. In the Pseudoalteromonas rubra treatment, the inoculum was detected at low relative abundance but induced a microbiome shift that persisted beyond the final inoculation. While no significant coral health benefits were observed in this short 17-day experiment, the Ruegeria arenilitoris strain unexpectedly induced tissue regression and mortality. The described experimental system offers a systematic approach for tracking bacterial dynamics in newly settled corals, facilitating the identification of potentially beneficial strains and the exclusion of harmful ones to inform the design of probiotic consortia for coral aquaculture.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 7","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70143","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144647077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Distribution of Iron Importers and Ecological Traits in Pseudomonas Species Highlights the Potential Role of Efe System in Plant-Related Environments 假单胞菌的铁输入物分布及其生态特性，揭示了铁系统在植物相关环境中的潜在作用

IF 4 2区生物学

Environmental microbiology Pub Date : 2025-07-16 DOI: 10.1111/1462-2920.70128

Kenji Okumura, Kohei Ogura, Wataru Hashimoto

{"title":"Distribution of Iron Importers and Ecological Traits in Pseudomonas Species Highlights the Potential Role of Efe System in Plant-Related Environments","authors":"Kenji Okumura, Kohei Ogura, Wataru Hashimoto","doi":"10.1111/1462-2920.70128","DOIUrl":"10.1111/1462-2920.70128","url":null,"abstract":"<div>\u0000 \u0000 Iron is essential for bacterial survival; however, the relationship of Pseudomonas species between iron import systems and environmental adaptation remains poorly understood. Multi-locus sequence and pan-genome analyses using 320 Pseudomonas genomes classified the species into four groups: P. aeruginosa, P. putida, P. syringae, and P. fluorescens, each exhibiting distinct patterns of iron importer and siderophore synthetic gene cluster. The P. aeruginosa group contained fewer iron importers, whereas the P. putida group exhibited a higher prevalence of xenosiderophore importers. The Fe2+-importing Efe system was predominantly detected in the P. syringae and P. fluorescens groups, both of which include plant pathogens and plant growth-promoting rhizobacteria. Reanalysis of publicly available transcriptome data revealed efeU, foxA, and fpvA were significantly upregulated in plant roots but not in insect hosts, and these genes were specifically suppressed during plant immune responses. Growth test and in planta competitive assay using P. fluorescens wild-type and Efe system-deficient mutant suggested that the Efe system gives an advantage under plant-related environments. Phylogenetic analysis indicated the Pseudomonas Efe system was inherited through vertical gene transfer from a common ancestor. Our comprehensive analysis revealed the distribution of iron importers across Pseudomonas groups, highlighting the importance of these systems in environmental adaptation.\u0000 </div>","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 7","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144647078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Confrontations of the Pathogenic Fungus Colletotrichum graminicola With a Biocontrol Bacterium or a Ubiquitous Fungus Trigger Synthesis of Secondary Metabolites With Lead Structures of Synthetic Fungicides 致病性真菌炭疽杆菌与生物防治菌或普遍存在的真菌的对抗引发合成杀菌剂含铅结构次级代谢物的合成

IF 4 2区生物学

Environmental microbiology Pub Date : 2025-07-14 DOI: 10.1111/1462-2920.70145

Bennet Rohan Fernando Devasahayam, Henriette Uthe, Yvonne Poeschl, Holger B. Deising

{"title":"Confrontations of the Pathogenic Fungus Colletotrichum graminicola With a Biocontrol Bacterium or a Ubiquitous Fungus Trigger Synthesis of Secondary Metabolites With Lead Structures of Synthetic Fungicides","authors":"Bennet Rohan Fernando Devasahayam, Henriette Uthe, Yvonne Poeschl, Holger B. Deising","doi":"10.1111/1462-2920.70145","DOIUrl":"10.1111/1462-2920.70145","url":null,"abstract":"Microbial biological control agents are increasingly used as an alternative to synthetic pesticides. The application of these microorganisms massively affects all members of plant-colonising microbial communities, including pathogenic fungi. In the majority of cases, the resulting competition for ecological niches is decided by the toxicity of microbial secondary metabolites (SMs) formed. In this study, we devised confrontation experiments employing the fungal maize pathogen Colletotrichum graminicola and antagonistic partners, that is the biocontrol bacterium Bacillus amyloliquefaciens and the ubiquitous ascomycete Aspergillus nidulans. Transcriptome studies uncovered strong de-regulation of the vast majority of the C. graminicola secondary metabolite biosynthetic gene clusters (SMBGCs), with 69% and 86% of these clusters de-regulated at confrontation sites with B. amyloliquefaciens or A. nidulans, respectively. In the biocontrol bacterium and in A. nidulans confronting the maize pathogen, 100% and 74% of the SMBGCs were transcriptionally de-regulated, respectively. Correspondingly, non-targeted high-resolution LC–MS/MS revealed a large repertoire of 1738 and 1466 novel features formed in the fungus–bacterium and fungus–fungus confrontation, respectively. Surprisingly, several of these belong to chemical classes with lead structures of synthetic fungicides.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 7","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70145","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144624610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling Crocosphaera Responses to Phosphorus Depletion: Insights From Genome Analysis and Functional Characterization 揭示鳄鱼对磷枯竭的反应：来自基因组分析和功能表征的见解

IF 4 2区生物学

Environmental microbiology Pub Date : 2025-07-14 DOI: 10.1111/1462-2920.70153

Chloé Caille, Sophie Rabouille, Eva Ortega-Retuerta, Yann Denis, Olivier Crispi, Barbara Marie, Mireille Pujo-Pay, Vladimir Daric, Emmanuel Talla, Amel Latifi

{"title":"Unveiling Crocosphaera Responses to Phosphorus Depletion: Insights From Genome Analysis and Functional Characterization","authors":"Chloé Caille, Sophie Rabouille, Eva Ortega-Retuerta, Yann Denis, Olivier Crispi, Barbara Marie, Mireille Pujo-Pay, Vladimir Daric, Emmanuel Talla, Amel Latifi","doi":"10.1111/1462-2920.70153","DOIUrl":"10.1111/1462-2920.70153","url":null,"abstract":"Unicellular, nitrogen-fixing cyanobacteria (UCYN) thrive and support primary production in oligotrophic oceans, playing a significant role in the marine nitrogen cycle. Crocosphaera sp., a model organism for studying marine nitrogen fixation, is adapted to low phosphate (Pi) concentrations. Yet, how Crocosphaera copes with Pi depletion is rather poorly understood. We present a genomics analysis of Pi stress-responsive genes in this genus, encompassing six C. watsonii and two strains isolated in coastal environments, C. subtropica and C. chwakensis. We identified genes involved in Pi signalling and uptake, and dissolved organic phosphorus (DOP) hydrolysis. Results showed different genetic potentials to cope with Pi scarcity between the Crocosphaera strains. Physiological monitoring of cultures of C. watsonii WH8501 exposed to Pi depletion highlighted a capacity to survive for at least nine days, albeit with a skewed C:N:P stoichiometry. Upon addition of DOP, cultures efficiently recovered to a growth rate and cell composition equivalent to those observed under favourable conditions. The concomitant transcription analysis revealed diel expression patterns of Pi-related genes and endogenous clock genes, suggesting a possible circadian regulation. Our data deepen our understanding of the growth strategies Crocosphaera employs in Pi-limited environments, offering broader insights into microbial resilience in marine ecosystems.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 7","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70153","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144624609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Herbicides Have Minimal and Variable Effects on the Structure and Function of Bacterial Communities in Agricultural Soils 除草剂对农业土壤细菌群落结构和功能的影响是微小的和可变的

IF 4 2区生物学

Environmental microbiology Pub Date : 2025-07-14 DOI: 10.1111/1462-2920.70148

Margaret Gaylord, Abigail Thompson, Franck E. Dayan, Andrew R. Kniss, Dave Reichert, Kristen Otto, Rebecca Larson, Pankaj Trivedi

{"title":"Herbicides Have Minimal and Variable Effects on the Structure and Function of Bacterial Communities in Agricultural Soils","authors":"Margaret Gaylord, Abigail Thompson, Franck E. Dayan, Andrew R. Kniss, Dave Reichert, Kristen Otto, Rebecca Larson, Pankaj Trivedi","doi":"10.1111/1462-2920.70148","DOIUrl":"10.1111/1462-2920.70148","url":null,"abstract":"Synthetic herbicides are relied on for weed management in the United States, and non-chemical alternatives, such as tillage, can be inefficient and expensive at an agricultural scale. Previous research examining the influence of weed management strategies on soil microbial communities has shown variability and inconsistency in experimental design and results. This study investigates the impact of repeated applications of glyphosate, a mixture of selective herbicides, and tillage on soil bacterial communities in sugar beet production across two locations in the United States. Using next-generation sequencing and various assays, we explore the effects on bacterial community structure, composition, and function related to nutrient cycling and soil health. Although transient and minor differences in bacterial community structure could be observed for herbicides and tillage, these statistical differences do not appear to be biologically relevant as the soil function was equivalent for all applied treatments. Our findings provide evidence that repeated herbicide usage does not directly impact soil health and function in sugar beet production. This study emphasises the need for well-replicated, field-realistic, and long-term experiments to understand the ecological consequences of herbicides and tillage in agroecosystems.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 7","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70148","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144624506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Species-Specific Exudates From the Harmful Algae Alexandrium minutum and Prymnesium parvum Drive the Structure of a Synthetic Microbial Community 有害藻类小亚历山大藻和小樱草藻的物种特异性分泌物驱动合成微生物群落的结构

IF 4 2区生物学

Environmental microbiology Pub Date : 2025-07-11 DOI: 10.1111/1462-2920.70150

Enora Briand, Malwenn Lassudrie, Jean-Baptiste Bérard, Cécile Jauzein, Claire Labry, Thomas Lacour, Charlotte Nef, Cyril Noël, Virginie Raimbault, Nathalie Schreiber, Manoëlla Sibat, Simon Tanniou, Damien Réveillon, Matthieu Garnier

{"title":"Species-Specific Exudates From the Harmful Algae Alexandrium minutum and Prymnesium parvum Drive the Structure of a Synthetic Microbial Community","authors":"Enora Briand, Malwenn Lassudrie, Jean-Baptiste Bérard, Cécile Jauzein, Claire Labry, Thomas Lacour, Charlotte Nef, Cyril Noël, Virginie Raimbault, Nathalie Schreiber, Manoëlla Sibat, Simon Tanniou, Damien Réveillon, Matthieu Garnier","doi":"10.1111/1462-2920.70150","DOIUrl":"10.1111/1462-2920.70150","url":null,"abstract":"<div>\u0000 \u0000 In this study, we examined the role of species-specific algal exudates in shaping bacterial community structure and interactions using synthetic microbial communities co-cultured with Alexandrium minutum and Prymnesium parvum. Cell-free algal exudates supported bacterial growth as the sole carbon source and revealed distinct exometabolomes unique to each algal species. These exometabolomes selectively influenced bacterial community composition, even among common copiotrophic taxa. Furthermore, co-culture experiments highlighted that the presence of algal cells drove further bacterial assembly, particularly within particle-attached communities, emphasising the role of close algal–bacterial interactions in structuring microbial consortia. Metabolomic analyses showed significant modulation of algal exudates by bacteria, with axenic cultures containing a broader and more diverse range of metabolites. This suggests microbial consumption, degradation or suppression of metabolite production under xenic conditions. Importantly, we observed a bacterial-mediated increase in extracellular paralytic shellfish toxins (PSTs) in A. minutum cultures. Exudates of both algal species displayed hemolytic activity, which was not affected by the presence of bacteria, suggesting bioactive extracellular compounds (BECs) production does not rely on bacteria. These findings underscore the complex and dynamic nature of algal–bacterial interactions, with implications for nutrient cycling, toxin dynamics and harmful algal bloom ecology.\u0000 </div>","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 7","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144606690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Methanotrophic Flexibility of ‘Ca. Methanoperedens’ and Its Interactions With Sulphate-Reducing Bacteria in the Sediment of Meromictic Lake Cadagno Ca的甲烷营养柔韧性。卡达尼奥分生湖沉积物中甲烷开孔菌及其与硫酸盐还原菌的相互作用

IF 4 2区生物学

Environmental microbiology Pub Date : 2025-07-10 DOI: 10.1111/1462-2920.70133

Maider J. Echeveste Medrano, Guangyi Su, Lucas A. Blattner, Pedro Leão, Irene Sánchez-Andrea, Mike S. M. Jetten, Cornelia U. Welte, Jakob Zopfi

{"title":"Methanotrophic Flexibility of ‘Ca. Methanoperedens’ and Its Interactions With Sulphate-Reducing Bacteria in the Sediment of Meromictic Lake Cadagno","authors":"Maider J. Echeveste Medrano, Guangyi Su, Lucas A. Blattner, Pedro Leão, Irene Sánchez-Andrea, Mike S. M. Jetten, Cornelia U. Welte, Jakob Zopfi","doi":"10.1111/1462-2920.70133","DOIUrl":"10.1111/1462-2920.70133","url":null,"abstract":"The greenhouse gas methane is an important contributor to global warming, with freshwater sediments representing important potential methane sources. Anaerobic methane-oxidising archaea mitigate methane release into the atmosphere by coupling the oxidation of methane to the reduction of extracellular electron acceptors or through interspecies electron transfer with microbial partners. Understanding their metabolic flexibility and microbial interactions is crucial to assess their role in global methane cycling. Here, we investigated anoxic sediments of the meromictic freshwater Lake Cadagno (Switzerland), where ‘Ca. Methanoperedens’ co-occur with a specific sulphate-reducing bacterium, with metagenomics and long-term incubations. Incubations were performed with different electron acceptors, revealing that manganese oxides supported highest CH4 oxidation potential but enriched for ‘Ca. Methanoperedens’ phylotypes that were hardly present in the inoculum. Combining data from the inoculum and incubations, we obtained five ‘Ca. Methanoperedens’ genomes, each harbouring different extracellular electron transfer pathways. In a reconstructed Desulfobacterota QYQD01 genome, we observed large multi-heme cytochromes, type IV pili, and a putative loss of hydrogenases, suggesting facultative syntrophic interactions with ‘Ca. Methanoperedens’. This research deepens our understanding of the metabolic flexibility and potential interspecific interactions of ‘Ca. Methanoperedens’ in freshwater lakes.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 7","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70133","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144598631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Wolbachia Feminises a Spider Host With Assistance From Co-Infecting Symbionts 沃尔巴克氏体在共感染共生体的帮助下使蜘蛛宿主雌性化

IF 4 2区生物学

Environmental microbiology Pub Date : 2025-07-10 DOI: 10.1111/1462-2920.70149

Virginija Mackevicius-Dubickaja, Yuval Gottlieb, Jennifer A. White, Matthew R. Doremus

{"title":"Wolbachia Feminises a Spider Host With Assistance From Co-Infecting Symbionts","authors":"Virginija Mackevicius-Dubickaja, Yuval Gottlieb, Jennifer A. White, Matthew R. Doremus","doi":"10.1111/1462-2920.70149","DOIUrl":"10.1111/1462-2920.70149","url":null,"abstract":"Arthropods commonly harbour maternally-transmitted bacterial symbionts that manipulate host biology. Multiple heritable symbionts can co-infect the same individual, allowing these host-restricted bacteria to engage in cooperation or conflict, which can ultimately affect host phenotype. The spider Mermessus fradeorum is infected with up to five heritable symbionts: Rickettsiella (R), Tisiphia (T), and three strains of Wolbachia (W1-3). Quintuply infected spiders are feminised, causing genetic males to develop as phenotypic females and produce almost exclusively female offspring. By comparing feminisation across nine infection combinations, we identified a Wolbachia strain, W1, that is required for feminisation. We also observed that spiders infected with both W1 and W3 produced ~10% more females than those lacking W3. This increase in feminisation rate does not seem to be due to direct changes in W1 titre, nor does W1 titre correlate with feminisation rate. Instead, we observed subtle titre interactions among symbionts, with lower relative abundance of R and T symbionts in strongly feminised infections. This synergistic effect of co-infection on Wolbachia feminisation may promote the spread of all five symbionts in spider populations. These results confirm the first instance of Wolbachia-induced feminisation in spiders and demonstrate that co-infecting symbionts can improve the efficacy of symbiont-induced feminisation.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 7","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70149","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144589681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0