Environmental microbiology最新文献_第8页

Insects as Natural Hosts, Vectors and Reservoirs of Botulinum Neurotoxin-Producing Clostridia and Their Non-Toxinogenic Counterparts: Preliminary Evidence 昆虫作为肉毒杆菌神经毒素产生梭菌及其非毒性对应体的天然宿主、媒介和宿主：初步证据

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-02-13 DOI: 10.1111/1462-2920.70053

François P. Douillard, Olivia Lanzoni, Anne Duplouy, Miia Lindström

{"title":"Insects as Natural Hosts, Vectors and Reservoirs of Botulinum Neurotoxin-Producing Clostridia and Their Non-Toxinogenic Counterparts: Preliminary Evidence","authors":"François P. Douillard, Olivia Lanzoni, Anne Duplouy, Miia Lindström","doi":"10.1111/1462-2920.70053","DOIUrl":"https://doi.org/10.1111/1462-2920.70053","url":null,"abstract":"<div>\u0000 \u0000 Insects play a significant role in the transmission and spread of bacterial pathogens that cause various diseases in humans and animals. The relationship among insects, bacterial pathogens and diseases is complex and depends on the specificity of the pathogens. Some clostridial species produce botulinum neurotoxin (BoNT), which is responsible for paralytic botulism. However, the ecology of these bacterial species and their non-toxinogenic phylogenetic counterparts remains unclear. This study specifically explored in silico evidence of the interconnection between BoNT-producing Clostridia and their non-toxinogenic counterparts with insects. Based on literature meta-analysis, the mining of 16S rRNA amplicon and metagenomic sequencing datasets and a pilot feeding experiment in the Glanville fritillary butterfly, Melitaea cinxia, we propose that BoNT-producing Clostridia and their non-toxinogenic phylogenetic counterparts are carried internally and/or externally in different insect orders. While previous case studies have indicated associations between Clostridia and insects, this work provides a more comprehensive view of their occurrence. It also highlights the need for further multidisciplinary investigations to characterise the natural ecology of BoNT-producing Clostridia and their non-toxinogenic counterparts in insects.\u0000 </div>","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 2","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143404447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phylogenetic and Functional Diversity of Soluble Di-Iron Monooxygenases 可溶性二铁单加氧酶的系统发育与功能多样性

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-02-13 DOI: 10.1111/1462-2920.70050

Sui Nin Nicholas Yang, Michael A. Kertesz, Nicholas V. Coleman

{"title":"Phylogenetic and Functional Diversity of Soluble Di-Iron Monooxygenases","authors":"Sui Nin Nicholas Yang, Michael A. Kertesz, Nicholas V. Coleman","doi":"10.1111/1462-2920.70050","DOIUrl":"https://doi.org/10.1111/1462-2920.70050","url":null,"abstract":"Monooxygenase (MO) enzymes are responsible for the oxidation of hydrocarbons and other compounds in the carbon and nitrogen cycles, are important for the biodegradation of pollutants and can act as biocatalysts for chemical manufacture. The soluble di-iron monooxygenases (SDIMOs) are of interest due to their broad substrate range, high enantioselectivity and ability to oxidise inert substrates such as methane. Here, we re-examine the phylogeny and functions of these enzymes, using recent advances in the field and expansions in sequence diversity in databases to highlight relationships between SDIMOs and revisit their classification. We discuss the impact of horizontal gene transfer on SDIMO phylogeny, the potential of SDIMOs for the biodegradation of pollutants and the importance of heterologous expression as a tool for understanding SDIMO functions and enabling their use as biocatalysts. Our analysis highlights current knowledge gaps, most notably, the unknown substrate ranges and physiological roles of enzymes that have so far only been detected via genome or metagenome sequencing. Enhanced understanding of the diversity and functions of the SDIMO enzymes will enable better prediction and management of biogeochemical processes and also enable new applications of these enzymes for biocatalysis and bioremediation.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 2","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70050","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143404445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Highly Dynamic Archaeal and Bacterial Communities From the Surface to the Deep in the Atlantic Ocean 从大西洋表层到深海的高度动态的古细菌和细菌群落

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-02-13 DOI: 10.1111/1462-2920.70039

Kristin M. Yoshimura, Sunita R. Shah Walter, Benjamin J. Tully, Jennifer F. Biddle

{"title":"Highly Dynamic Archaeal and Bacterial Communities From the Surface to the Deep in the Atlantic Ocean","authors":"Kristin M. Yoshimura, Sunita R. Shah Walter, Benjamin J. Tully, Jennifer F. Biddle","doi":"10.1111/1462-2920.70039","DOIUrl":"https://doi.org/10.1111/1462-2920.70039","url":null,"abstract":"<div>\u0000 \u0000 Though archaea are ubiquitous in the oceans, at times present in quantities equal to bacteria, in general, archaea are underreported when compared with bacteria, and little is known about archaeal connectivity in the ocean. Here, we present the first time series data of size-fractionated archaea with temporal resolution of days across a depth transect in the oligotrophic Atlantic Ocean. Archaeal communities were variable from day to day, both in regards to community composition and relative abundance. In the context of the total prokaryotic community, we found that water depth and particle size were both determinants of the archaeal share of microbial populations, though the archaeal portion of the community was less discriminating between size and depth classes than the rest of the community. Quantitative PCR shows lower relative abundances of archaea in the deep Atlantic Ocean than previously reported. Overall, we find far lower connectivity between depths in the Atlantic Ocean than suggested by previous studies.\u0000 </div>","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 2","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143404446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Invertebrate Decline Has Minimal Effects on Oak-Associated Microbiomes 无脊椎动物的减少对橡树相关微生物群的影响微乎其微

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-02-12 DOI: 10.1111/1462-2920.70051

Cynthia Albracht, François Buscot, Nico Eisenhauer, Alban Gebler, Sylvie Herrmann, Anja Schmidt, Mika Tarkka, Kezia Goldmann

{"title":"Invertebrate Decline Has Minimal Effects on Oak-Associated Microbiomes","authors":"Cynthia Albracht, François Buscot, Nico Eisenhauer, Alban Gebler, Sylvie Herrmann, Anja Schmidt, Mika Tarkka, Kezia Goldmann","doi":"10.1111/1462-2920.70051","DOIUrl":"https://doi.org/10.1111/1462-2920.70051","url":null,"abstract":"Recently, biomass of invertebrates has declined substantially at many locations with the implications of this biodiversity loss for ecosystems yet unknown. Through multitrophic interactions, plant- and soil-associated microbiomes might be altered, causing a cascade of changes on diverse ecosystem processes. We simulated aboveground invertebrate decline in grassland ecosystems with two levels of invertebrate biomass (36% and 100% of current ambient conditions), plus a control with no invertebrates present. Each standardised grassland mesocosm additionally contained one clonal Quercus robur L. sapling to investigate the extent of invertebrate decline effects exceeding grasslands. We investigated oak biomass partitioning and mycorrhiza formation, oak leaf transcriptome and microbiome composition of leaves, roots and rhizosphere. While invertebrate decline did not significantly affect oak performance and herbivory-related gene expression, fungal communities presented an increase of saprotrophs and pathogens, especially in leaves. Among leaf-inhabiting bacteria, Proteobacteria and Actinobacteria increased under invertebrate decline. The belowground microbiome was only little affected. But, invertebrate decline came along with a reduced influence on predators leading to an elevated aphids infestation that proofed able to alter microbiota. Our findings establish a strong difference between above- and belowground, with the impacts of invertebrate decline being more pronounced in the leaf microbiome.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 2","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70051","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143396952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Persistent Desert Microbiota in the Southern European Sky 南欧天空中持续存在的沙漠微生物群

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-02-09 DOI: 10.1111/1462-2920.70046

Joan Cáliz, Mateu Menéndez-Serra, Xavier Triadó-Margarit, Anna Avila, Emilio O. Casamayor

{"title":"Persistent Desert Microbiota in the Southern European Sky","authors":"Joan Cáliz, Mateu Menéndez-Serra, Xavier Triadó-Margarit, Anna Avila, Emilio O. Casamayor","doi":"10.1111/1462-2920.70046","DOIUrl":"10.1111/1462-2920.70046","url":null,"abstract":"<div>\u0000 \u0000 Long-range atmospheric processes facilitate global microbial dispersal, with a pivotal role in Earth's ecosystem functioning and global health. Aerobiological studies have traditionally focused on low troposphere aerosols, leading to the assumption that airborne communities are primarily controlled by neighbouring ecosystems. We show a temporal sampling of aerosols from the free troposphere extending a period of almost three decades, coupled with the study of both high troposphere air masses provenances and genetic data of topsoils from North Africa and from a global public bacterial database. The results unveil a long-lasting influence of airborne North African desert microorganisms in Southern Europe. Although sea spray dominates global aerosol emissions, the predominance of desert microorganisms was widespread even in rain traced back to the Atlantic Ocean. The frequency of dust outbreaks, altitude reached, and long residence times are postulated as critical factors that significantly shape the long-range and persistence of aerial assemblages, with air mass provenance playing a secondary role. This study advances the current understanding of atmospheric microorganisms, underscoring their close and long-lasting relationship with terrestrial ecosystems. Further research is needed to fully understand intercontinental aerial connections with deserts and drylands elsewhere, and the influence of desert immigrants on worldwide ecosystems.\u0000 </div>","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 2","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143375376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Shallow Hydrothermal Fluids Shape Microbial Dynamics at the Tagoro Submarine Volcano (Canary Islands, Spain) 塔戈罗海底火山浅层热液形成微生物动力学（西班牙加那利群岛）

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-02-09 DOI: 10.1111/1462-2920.70052

Clàudia Pérez-Barrancos, Eugenio Fraile-Nuez, Juan Pablo Martín-Díaz, Alba González-Vega, José Escánez-Pérez, María Isabel Díaz-Durán, Carmen Presas-Navarro, Mar Nieto-Cid, Jesús María Arrieta

{"title":"Shallow Hydrothermal Fluids Shape Microbial Dynamics at the Tagoro Submarine Volcano (Canary Islands, Spain)","authors":"Clàudia Pérez-Barrancos, Eugenio Fraile-Nuez, Juan Pablo Martín-Díaz, Alba González-Vega, José Escánez-Pérez, María Isabel Díaz-Durán, Carmen Presas-Navarro, Mar Nieto-Cid, Jesús María Arrieta","doi":"10.1111/1462-2920.70052","DOIUrl":"10.1111/1462-2920.70052","url":null,"abstract":"Shallow underwater hydrothermal systems are often overlooked despite their potential contribution to marine diversity and biogeochemistry. Over a decade after its eruption, the Tagoro submarine volcano continues to emit heat, reduced compounds, and nutrients into shallow waters, serving as a model system for studying the effects of diffuse hydrothermal fluids on surface microbial communities. The impact on both phytoplankton and bacterial communities was examined through experimental manipulations mimicking dilution levels up to ~100 m from the primary crater of Tagoro. Chlorophyll a concentration doubled in the presence of hydrothermal products, with peak levels detected about a day earlier than in controls. Picoeukaryotes and Synechococcus cell abundances moderately increased, yet small eukaryotic phytoplankton (≤ 5 μm) predominated in the hydrothermally enriched bottles. Dinoflagellates, diatoms, small green algae and radiolarians particularly benefited from the hydrothermal inputs, along with phototrophic and chemoautotrophic bacteria. Our results indicate that hydrothermal products in shallow waters enhance primary production driven by phototrophic microbes, potentially triggering a secondary response associated with increased organic matter availability. Additionally, protistan grazing and parasitism emerged as key factors modulating local planktonic communities. Our findings highlight the role of shallow submarine hydrothermal systems in enhancing local primary production and element cycling.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 2","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70052","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143375377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Volatile Organic Compounds of Diverse Origins and Their Changes Associated With Cultivar Decay in a Fungus-Farming Termite 不同来源的挥发性有机化合物及其与菌种栽培白蚁腐烂相关的变化

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-02-05 DOI: 10.1111/1462-2920.70049

Nanna Hjort Vidkjær, Suzanne Schmidt, Cleo Lisa Davie-Martin, Kolotchèlèma Simon Silué, N'golo Abdoulaye Koné, Riikka Rinnan, Michael Poulsen

{"title":"Volatile Organic Compounds of Diverse Origins and Their Changes Associated With Cultivar Decay in a Fungus-Farming Termite","authors":"Nanna Hjort Vidkjær, Suzanne Schmidt, Cleo Lisa Davie-Martin, Kolotchèlèma Simon Silué, N'golo Abdoulaye Koné, Riikka Rinnan, Michael Poulsen","doi":"10.1111/1462-2920.70049","DOIUrl":"10.1111/1462-2920.70049","url":null,"abstract":"Fungus-farming termites cultivate a Termitomyces fungus monoculture in enclosed gardens (combs) free of other fungi, except during colony declines, where Pseudoxylaria spp. stowaway fungi appear and take over combs. Here, we determined Volatile Organic Compounds (VOCs) of healthy Macrotermes bellicosus nests in nature and VOC changes associated with comb decay during Pseudoxylaria takeover. We identified 443 VOCs and unique volatilomes across samples and nest volatilomes that were mainly composed of fungus comb VOCs with termite contributions. Few comb VOCs were linked to chemical changes during decay, but longipinocarvone and longiverbenone were only emitted during comb decay. These terpenes may be involved in Termitomyces defence against antagonistic fungi or in fungus-termite signalling of comb state. Both comb and Pseudoxylaria biomass volatilomes contained many VOCs with antimicrobial activity that may serve in maintaining healthy Termitomyces monocultures or aid in the antagonistic takeover by Pseudoxylaria during colony decline. We further observed a series of oxylipins with known functions in the regulation of fungus germination, growth, and secondary metabolite production. Our volatilome map of the fungus-farming termite symbiosis provides new insights into the chemistry regulating complex interactions and serves as a valuable guide for future work on the roles of VOCs in symbioses.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 2","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70049","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143192106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic Transfer in Action: Uncovering DNA Flow in an Extremophilic Microbial Community 行动中的遗传转移：揭示嗜极微生物群落中的DNA流动

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-02-03 DOI: 10.1111/1462-2920.70048

Julia Van Etten, Timothy G. Stephens, Debashish Bhattacharya

{"title":"Genetic Transfer in Action: Uncovering DNA Flow in an Extremophilic Microbial Community","authors":"Julia Van Etten, Timothy G. Stephens, Debashish Bhattacharya","doi":"10.1111/1462-2920.70048","DOIUrl":"10.1111/1462-2920.70048","url":null,"abstract":"Horizontal genetic transfer (HGT) is a significant driver of genomic novelty in all domains of life. HGT has been investigated in many studies however, the focus has been on conspicuous protein-coding DNA transfers that often prove to be adaptive in recipient organisms and are therefore fixed longer-term in lineages. These results comprise a subclass of HGTs and do not represent exhaustive (coding and non-coding) DNA transfer and its impact on ecology. Uncovering exhaustive HGT can provide key insights into the connectivity of genomes in communities and how these transfers may occur. In this study, we use the term frequency-inverse document frequency (TF-IDF) technique, that has been used successfully to mine DNA transfers within real and simulated high-quality prokaryote genomes, to search for exhaustive HGTs within an extremophilic microbial community. We establish a pipeline for validating transfers identified using this approach. We find that most DNA transfers are within-domain and involve non-coding DNA. A relatively high proportion of the predicted protein-coding HGTs appear to encode transposase activity, restriction-modification system components, and biofilm formation functions. Our study demonstrates the utility of the TF-IDF approach for HGT detection and provides insights into the mechanisms of recent DNA transfer.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 2","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70048","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143083566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Temporal and Spatial Dynamics of Synechococcus Clade II and Other Microbes in the Eutrophic Subtropical San Diego Bay 圣地亚哥湾富营养化亚热带聚囊球菌及其他微生物的时空动态

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-02-03 DOI: 10.1111/1462-2920.70043

Katie J. Harding, Maitreyi Nagarkar, Maggie Wang, Kailey Ramsing, Niv Anidjar, Sarah Giddings, Bianca Brahamsha, Brian Palenik

{"title":"Temporal and Spatial Dynamics of Synechococcus Clade II and Other Microbes in the Eutrophic Subtropical San Diego Bay","authors":"Katie J. Harding, Maitreyi Nagarkar, Maggie Wang, Kailey Ramsing, Niv Anidjar, Sarah Giddings, Bianca Brahamsha, Brian Palenik","doi":"10.1111/1462-2920.70043","DOIUrl":"10.1111/1462-2920.70043","url":null,"abstract":"The diversity of the marine cyanobacterium Synechococcus can be broadly separated into clades, with clade II typically present in warm oligotrophic water, and clades I and IV found in cooler coastal water. We found amplicon sequence variants (ASVs) belonging to clade II in the nutrient-replete waters of San Diego Bay (SDB). Using the 16S rRNA gene, 18S rRNA gene and internal transcribed spacer region sequencing, we analysed multiple locations in SDB monthly for over a year, with additional samples dating back to 2015. Synechococcus community composition differed from the nearby coast into SDB in terms of dominant clade and ASVs. Specific clade II ASVs became relatively more abundant towards the back of the bay and showed seasonality, with higher relative abundance in the warm months. Select ASVs group phylogenetically and show similar seasonal and spatial distribution patterns, indicating these ASVs have adapted to SDB. Isolates matching clade II ASVs from SDB show pigment composition that is better adapted to the green light available in SDB, further supporting our findings. Other microbial taxa also show SDB enrichment, providing evidence that SDB is a chemostat-like environment where circulation, temperature, light and other environmental conditions create a zone for microbial evolution and diversification.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 2","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70043","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143083567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacterial Diversity of Marine Biofilm Communities in Terra Nova Bay (Antarctica) by Culture-Dependent and -Independent Approaches 南极特拉诺瓦湾海洋生物膜群落细菌多样性的培养依赖和独立研究

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-02-02 DOI: 10.1111/1462-2920.70045

Bisaccia Melissa, Binda Elisa, Caruso Gabriella, Azzaro Maurizio, Dell' Acqua Ombretta, Di Cesare Andrea, Ester Maria Eckert, Marinelli Flavia

{"title":"Bacterial Diversity of Marine Biofilm Communities in Terra Nova Bay (Antarctica) by Culture-Dependent and -Independent Approaches","authors":"Bisaccia Melissa, Binda Elisa, Caruso Gabriella, Azzaro Maurizio, Dell' Acqua Ombretta, Di Cesare Andrea, Ester Maria Eckert, Marinelli Flavia","doi":"10.1111/1462-2920.70045","DOIUrl":"10.1111/1462-2920.70045","url":null,"abstract":"Applying both culture-independent and -dependent approaches, bacterial diversity of marine biofilm communities colonising polyvinyl chloride panels submerged in Terra Nova Bay (Ross Sea, Antarctica) was investigated. Panels were deployed in two sites subjected to a different degree of anthropogenic impact (Road Bay [RB] impacted site and Punta Stocchino [PTS] control site). Biofilm samples were collected after 3 or 12 months to evaluate both short- and long-term microbial colonisation. Taxonomic composition of the microbial community was studied by 16S rRNA gene amplicon sequencing. Proteobacteria was the predominant phylum, followed by Bacteroidetes, Actinobacteria, Verrucomicrobia and Firmicutes. Impacted RB biofilms were found to contain a relevant fraction of potentially pathogenic bacterial genera, accounting for 27.49% of the whole community. A total of 86 psychrotolerant bacterial strains were isolated from the biofilm samples using culture-dependent techniques designed to enrich in Actinobacteria. These strains were assigned to three different phyla: Actinobacteria (54.65%), Firmicutes (32.56%) and Proteobacteria (12.79%). 2.73% of genera identified by metabarcoding were recovered also through cultivation, while 11 additional genera were uniquely yielded by cultivation. Functional screening of the isolates revealed their hydrolytic and oxidative enzyme activity patterns, giving new insights into the metabolic and biotechnological potential of microbial biofilm communities in Terra Nova Bay seawater.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 2","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70045","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143077200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0