Environmental microbiology最新文献_第8页

Metabolic Insights Into Microbially Induced Calcite Formation by Bacillaceae for Application in Bio-Based Construction Materials 杆菌科微生物诱导方解石形成的代谢研究及其在生物基建筑材料中的应用

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-04-02 DOI: 10.1111/1462-2920.70093

Michael Seidel, Charlotte Hamley-Bennett, Bianca J. Reeksting, Manpreet Bagga, Lukas Hellmann, Timothy D. Hoffmann, Christiane Kraemer, Irina Dana Ofiţeru, Kevin Paine, Susanne Gebhard

{"title":"Metabolic Insights Into Microbially Induced Calcite Formation by Bacillaceae for Application in Bio-Based Construction Materials","authors":"Michael Seidel, Charlotte Hamley-Bennett, Bianca J. Reeksting, Manpreet Bagga, Lukas Hellmann, Timothy D. Hoffmann, Christiane Kraemer, Irina Dana Ofiţeru, Kevin Paine, Susanne Gebhard","doi":"10.1111/1462-2920.70093","DOIUrl":"https://doi.org/10.1111/1462-2920.70093","url":null,"abstract":"Microbially induced calcite precipitation (MICP) offers promising solutions for sustainable, low-cement infrastructure materials. While it is known how urea catabolism leads to biomineralisation, the non-ureolytic pathways of MICP are less clear. This limits the use of the latter in biotechnology, despite its clear benefit of avoiding toxic ammonia release. To address this knowledge gap, the present study explored the interdependence between carbon source utilisation and non-ureolytic MICP. We show that acetate can serve as the carbon source driving calcite formation in several environmental Bacillaceae isolates. This effect was particularly clear in a Solibacillus silvestris strain, which could precipitate almost all provided calcium when provided with a 2:1 acetate-to-calcium molar ratio, and we show that this process was independent of active cell growth. Genome sequencing and gene expression analyses revealed an apparent link between acetate catabolism and calcite precipitation in this species, suggesting MICP may be a calcium stress response. Development of a simple genetic system for S. silvestris led to the deletion of a proposed calcium binding protein, although this showed minimal effects on MICP. Taken together, this study provides insights into the physiological processes leading to non-ureolytic MICP, paving the way for targeted optimisation of biomineralisation for sustainable materials development.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 4","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70093","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143762204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metagenome-Assembled Genomes and Metatranscriptome Analysis of Perfluorooctane Sulfonate-Reducing Bacteria Enriched From Activated Sludge 活性污泥富集的全氟辛烷磺酸还原菌的宏基因组组装基因组和宏转录组分析

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-04-01 DOI: 10.1111/1462-2920.70087

Sovannlaksmy Sorn, Norihisa Matsuura, Ryo Honda

{"title":"Metagenome-Assembled Genomes and Metatranscriptome Analysis of Perfluorooctane Sulfonate-Reducing Bacteria Enriched From Activated Sludge","authors":"Sovannlaksmy Sorn, Norihisa Matsuura, Ryo Honda","doi":"10.1111/1462-2920.70087","DOIUrl":"https://doi.org/10.1111/1462-2920.70087","url":null,"abstract":"Per- and polyfluoroalkyl substances (PFAS) exhibit a widespread distribution across diverse global ecosystems throughout their lifecycle, posing substantial risks to human health. The persistence of PFAS makes biodegradation a challenging yet environmentally friendly solution for their treatment. In the authors' previous study, a bacterial consortium capable of reducing perfluorooctane sulfonate (PFOS) was successfully enriched from activated sludge. This study aimed to investigate the array of genes associated with PFOS reduction via biosorption and biotransformation to elucidate the metabolic pathways. Two metagenome-assembled genomes (MAGs) based on 16S rRNA sequences that share 99.86% and 97.88% similarity with Hyphomicrobium denitrificans and Paracoccus yeei, respectively were obtained. They were found to contain several genes encoding enzymes that potentially regulate biofilm formation of biosorption and facilitate the desulfonation and defluorination processes of biotransformation. Transcriptomic analysis demonstrated the high expression levels of these genes, including alkanesulfonate monooxygenase, catechol dioxygenase, (S)-2-haloacid dehalogenase and putative cytochrome P450, suggesting their involvement in PFOS biotransformation. The expression of these genes supports the presence of candidate metabolites of PFOS biotransformation detected in the previous study. These findings emphasise the significant potential of bacterial consortia and the crucial role played by genes encoding enzymes in facilitating the remediation of PFOS contaminants.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 4","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70087","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143749424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic Analysis of Polyunsaturated Fatty Acids Biosynthesis Pathway Determines Four Distinct Thraustochytrid Types 多不饱和脂肪酸生物合成途径的遗传分析决定了四种不同的Thraustochytrid类型

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-03-28 DOI: 10.1111/1462-2920.70090

Sou-Yu Cheng, Yi-Jing Chen, Hsiu-Chin Lin, Hsin-Yang Chang, Ming-Der Huang

{"title":"Genetic Analysis of Polyunsaturated Fatty Acids Biosynthesis Pathway Determines Four Distinct Thraustochytrid Types","authors":"Sou-Yu Cheng, Yi-Jing Chen, Hsiu-Chin Lin, Hsin-Yang Chang, Ming-Der Huang","doi":"10.1111/1462-2920.70090","DOIUrl":"https://doi.org/10.1111/1462-2920.70090","url":null,"abstract":"Thraustochytrids, diverse marine unicellular protists encompassing over 10 recognised genera, are renowned for synthesising polyunsaturated fatty acids (PUFAs), with content and composition varying substantially across genera. While PUFAs are known to be produced via PUFA synthase (PUFA-S) and/or elongase/desaturase (ELO/DES) pathways, the distinctions in genes involved remain unexplored. This study analysed PUFA biosynthetic genes in 19 thraustochytrid strains across six genera, categorising them into four types. Type I exclusively utilises the ELO/DES pathway, Type II employs both PUFA-S and complete ELO/DES pathways, while Types III and IV primarily rely on PUFA-S, with Type III lacking the canonical Δ9 desaturase and Type IV missing most desaturase and elongase enzymes. Notably, the Δ9 desaturase and ATP-citrate lyase (ACLY) are exclusive to Types I and II, while β-carotene hydroxylase (CrtZ) is absent in these types. ACLY absence suggests alternative acetyl-CoA supply pathways in Types III and IV, whereas CrtZ absence implies either a lack of specific xanthophylls or alternative biosynthetic pathways in Types I and II. Synteny analysis revealed conserved genomic organisation of PUFA biosynthetic genes, indicating a shared evolutionary trajectory. This study provides insights into the genetic diversity underlying PUFA biosynthesis in thraustochytrids, while proposing putative evolutionary pathways for the four lineages.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 4","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70090","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Continuous Rice Cultivation Increases Celery Yield by Enhancing Plant Beneficial Bacteria in Rice-Celery Rotations 水稻连作通过增加水稻-芹菜轮作中植物有益菌群提高芹菜产量

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-03-28 DOI: 10.1111/1462-2920.70085

Danyan Qiu, Mingjing Ke, Nuohan Xu, Hang Hu, Yuke Zhu, Tao Lu, MingKang Jin, Zhenyan Zhang, Qi Zhang, Josep Penuelas, Michael Gillings, Haifeng Qian

{"title":"Continuous Rice Cultivation Increases Celery Yield by Enhancing Plant Beneficial Bacteria in Rice-Celery Rotations","authors":"Danyan Qiu, Mingjing Ke, Nuohan Xu, Hang Hu, Yuke Zhu, Tao Lu, MingKang Jin, Zhenyan Zhang, Qi Zhang, Josep Penuelas, Michael Gillings, Haifeng Qian","doi":"10.1111/1462-2920.70085","DOIUrl":"https://doi.org/10.1111/1462-2920.70085","url":null,"abstract":"<div>\u0000 \u0000 The sustainable management of crops is a fundamental challenge as the human population and demand for food increase. Crop rotation, a practice that has been used for centuries, offers a sustainable solution with minimal environmental impact. However, our understanding of how microbial diversity changes during rotation and how microbially mediated functions enhance plant production remains limited. In our study, we combined field surveys of rice–celery rotations with greenhouse experiments. We found that crop rotation increased yield by increasing the presence of plant-beneficial bacteria, including a novel strain named Acinetobacter bohemicus HfQ1. Bacteria that promote plant growth are enriched under crop rotation, leading to increased ammonia oxidation, siderophore production and indole-3-acetic acid synthesis. These beneficial ecological consequences of crop rotation were consistent across various crops during our metadata analysis. Our study provides new insights into the development of innovative crop rotation models and effective strategies to safeguard food production and advance sustainable agriculture. Additionally, the Acinetobacter strain may serve as a potential microbial agent to replace chemical fertilisers, further supporting sustainable agricultural practices.\u0000 </div>","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 4","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Flowering Plant Microbiomes and Network Interactions Across an Urban Gradient 开花植物微生物组和城市梯度的网络相互作用

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-03-28 DOI: 10.1111/1462-2920.70089

Katherine D. Chau, Makaylee K. Crone, Phuong N. Nguyen, Sandra M. Rehan

{"title":"Flowering Plant Microbiomes and Network Interactions Across an Urban Gradient","authors":"Katherine D. Chau, Makaylee K. Crone, Phuong N. Nguyen, Sandra M. Rehan","doi":"10.1111/1462-2920.70089","DOIUrl":"https://doi.org/10.1111/1462-2920.70089","url":null,"abstract":"We used flowers to explore how ephemeral anthosphere microbiomes differ among flowering plant species and along an urban gradient. Here, we sequenced 16S rRNA for bacteria, ITS1 for fungi and rbcL for plant DNA from 10 different plant species sampled to characterise anthosphere microbiomes along an urban gradient and identify important network interactions. Bacterial and fungal flower microbiomes significantly differed in diversity across plant species, especially among Asteraceae and Fabaceae. Across all analyses, four taxa, the bacteria Pantoea and Rosenbergiella and the fungi Alternaria and Cladosporium were highly prevalent and contributed to the majority of microbiome composition differences observed between plant species. These four taxa harbour strains or species that may be either pathogenic or beneficial to plants. Across a land use gradient, the plant community bacterial and fungal microbiome was stable and consistent. Flower-plant networks confirmed all focal flower families in abundance on each sampled flower, with the addition of Paulowniaceae, suggesting that pollinators visiting the focal flowers also visit this plant family. Our findings reveal that anthosphere microbiomes are diverse at the plant community level and encouragingly remain robust against urbanisation.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 4","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70089","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diterpenoid Phytoalexins Shape Rice Root Microbiomes and Their Associations With Root Parasitic Nematodes 二萜类植物抗毒素影响水稻根系微生物群及其与根系寄生线虫的关系

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-03-28 DOI: 10.1111/1462-2920.70084

Enoch Narh Kudjordjie, Willem Desmedt, Tina Kyndt, Mogens Nicolaisen, Reuben J. Peters, Mette Vestergård

{"title":"Diterpenoid Phytoalexins Shape Rice Root Microbiomes and Their Associations With Root Parasitic Nematodes","authors":"Enoch Narh Kudjordjie, Willem Desmedt, Tina Kyndt, Mogens Nicolaisen, Reuben J. Peters, Mette Vestergård","doi":"10.1111/1462-2920.70084","DOIUrl":"https://doi.org/10.1111/1462-2920.70084","url":null,"abstract":"Rice synthesises diterpenoid phytoalexins (DPs) which are known to operate in defence against foliar microbial pathogens and the root-knot nematode Meloidogyne graminicola. Here, we examined the role of DPs in shaping rice-associated root microbiomes in nematode-infested field soil. Further, we assessed how DPs affect interactions between the root microbiomes and M. graminicola. We used 16S and ITS2 rRNA gene amplicon analysis to characterise the root- and rhizosphere-associated microbiomes of DP knock-out rice mutants and their wild-type parental line, at an early (17 days) and late (28 days) stage of plant development in field soil. Disruption of DP synthesis resulted in distinct changes in the composition and structure of microbial communities both relative to the parental/wild-type line but also between individual mutants, indicating specificity in DP-microbe interactions. Moreover, the abundance of nematode-suppressive microbial taxa, including Streptomyces, Stenotrophomonas and Enterobacter was negatively correlated with that of Meloidogyne. Differential enrichment of microbial taxa in the roots of rice DP knock-out mutants versus wild-type suggests that DPs modulate specific taxa in the rice root microbiome. These findings indicate a role for DPs in plant-microbiome assembly and nematode interactions, further underscoring the potential of leveraging phytoalexins for sustainable management of crop diseases.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 4","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70084","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cyanophage Lysis of the Cyanobacterium Nodularia spumigena Affects the Variability and Fitness of the Host-Associated Microbiome 蓝藻结核菌的噬藻裂解影响宿主相关微生物组的变异性和适应性

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-03-28 DOI: 10.1111/1462-2920.70042

Sigitas Šulčius, Gediminas Alzbutas, Valiantsin Lukashevich

{"title":"Cyanophage Lysis of the Cyanobacterium Nodularia spumigena Affects the Variability and Fitness of the Host-Associated Microbiome","authors":"Sigitas Šulčius, Gediminas Alzbutas, Valiantsin Lukashevich","doi":"10.1111/1462-2920.70042","DOIUrl":"https://doi.org/10.1111/1462-2920.70042","url":null,"abstract":"<div>\u0000 \u0000 Cyanobacteria are intricately linked with its microbiome through multiple metabolic interactions. We assessed how these interactions might be affected by cyanophage infection and lysis in cyanobacterium Nodularia spumigena. The genome-scale metabolic models and analysis of putative metabolic interactions revealed a bidirectional cross-feeding potential within the N. spumigena microbiome, with heterotrophic bacteria exhibiting a greater level of trophic dependency on the cyanobacterium. Our results indicate that microbes associated with N. spumigena rely on the supply of various amino acids, reduced carbon compounds and protein synthesis cofactors released by the cyanobacterial host. We observed that compositional changes in the N. spumigena microbiome were associated with the multiplicity of infection and increased with increasing initial viral load. Higher mortality of N. spumigena led to decreased variability in the relative abundances of bacteria, suggesting an indirect restriction of their niche space. Lysis of N. spumigena resulted in a substantial decline in the estimated absolute abundances of heterotrophic bacteria, indicating reduced fitness of co-occurring bacteria in the absence of N. spumigena. Altogether, we demonstrate how a gradual increase in viral pressure on the photosynthetic host propagates through the co-occurring microbial community, disrupting cooperative nature and microbial connectivity within the N. spumigena microbiome.\u0000 </div>","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 4","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tracking Different States of Spiked Environmental DNA Using Multiplex Digital PCR Assays 使用多重数字PCR分析跟踪不同状态的加标环境DNA

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-03-28 DOI: 10.1111/1462-2920.70086

Julia Zöhrer, Judith Ascher-Jenull, Andreas O. Wagner

{"title":"Tracking Different States of Spiked Environmental DNA Using Multiplex Digital PCR Assays","authors":"Julia Zöhrer, Judith Ascher-Jenull, Andreas O. Wagner","doi":"10.1111/1462-2920.70086","DOIUrl":"https://doi.org/10.1111/1462-2920.70086","url":null,"abstract":"The study of microbial communities based on the total environmental DNA (eDNA) is influenced by the presence of different eDNA states, i.e., intracellular (iDNA) and extracellular DNA (exDNA), and the choice of the DNA extraction method. Although the use of spike-and-recovery controls facilitates the diagnosis of such issues, appropriate experimental setups simultaneously accounting for the different eDNA states and their bacterial origins are missing. Here, we used two single-gene deletion mutants of both Escherichia coli and Bacillus subtilis to trace exDNA and iDNA spike-ins of each selected model organism within environmental samples. Unique primer/probe sets were developed for each strain, allowing their absolute quantification using multiplex digital PCR assays. The proposed spike-and-recovery controls were successfully applied to various environments including soil, sediment, sludge and compost. While the percent recovery of spiked iDNA differed significantly between E. coli and B. subtilis, results were similar for both model organisms in the case of spiked exDNA, emphasising that the fate of DNA molecules in the environment is similar irrespective of their bacterial origin. Hence, future studies may benefit from the proposed approach to better understand methodological ambiguities related to the eDNA extraction in general as well as the separation of the different eDNA states.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 4","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70086","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacteriophage–Bacteria Interactions Promote Ecological Multifunctionality in Compost-Applied Soils 噬菌体-细菌相互作用促进堆肥土壤的生态多功能性

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-03-20 DOI: 10.1111/1462-2920.70074

Shimao Wu, Wen Zhang, Danrui Wang, Jose Luis Balcazar, Guanghao Wang, Mao Ye, Huizhen Chao, Mingming Sun, Feng Hu

{"title":"Bacteriophage–Bacteria Interactions Promote Ecological Multifunctionality in Compost-Applied Soils","authors":"Shimao Wu, Wen Zhang, Danrui Wang, Jose Luis Balcazar, Guanghao Wang, Mao Ye, Huizhen Chao, Mingming Sun, Feng Hu","doi":"10.1111/1462-2920.70074","DOIUrl":"10.1111/1462-2920.70074","url":null,"abstract":"<div>\u0000 \u0000 Bacteriophages (phages) influence biogeochemical cycling in soil ecosystems by mediating bacterial metabolism. However, the participation of phages in soil's overall ecological functions (multifunctionality) remains unclear. Hence, this study investigated the potential for phages and bacterial communities to shape the multifunctionality of compost-applied soils. The findings revealed that cow compost and vermicompost applications enhanced the soil's multifunctionality; consequently, the highest multifunctionality was observed in the soil with vermicompost application (p < 0.05). The composition and diversity of bacteria and phages, as well as the abundance of functional genes of bacteria and phages related to carbon, nitrogen, phosphorus and sulphur metabolism, were dramatically altered following the application of both compost types. Moreover, the impact of phage diversity on soil multifunctionality is crucial for multi-threshold calculations. Structural equation modelling indicated that the effects of bacterial diversity on soil multifunctionality following compost application were paramount, with a path coefficient of 0.88 (p < 0.01). The rise in phage diversity and the enrichment of functional genes indirectly led to a dramatic increase in the soil's ecological multifunctionality by affecting the host bacteria's metabolic processes. These results offer a novel avenue to improve soil's functions and environmental services by transforming the phage community composition and functions of soils.\u0000 </div>","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 3","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143660634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial Community Metabolism of Coral Reef Exometabolomes Broadens the Chemodiversity of Labile Dissolved Organic Matter 珊瑚礁外代谢组的微生物群落代谢扩大了不稳定溶解有机质的化学多样性

IF 4.3 2区生物学

Environmental microbiology Pub Date : 2025-03-19 DOI: 10.1111/1462-2920.70064

Zachary A. Quinlan, Craig E. Nelson, Irina Koester, Daniel Petras, Louis-Felix Nothias, Jacqueline Comstock, Brandie M. White, Lihini I. Aluwihare, Barbara A. Bailey, Craig A. Carlson, Pieter C. Dorrestein, Andreas F. Haas, Linda Wegley Kelly

{"title":"Microbial Community Metabolism of Coral Reef Exometabolomes Broadens the Chemodiversity of Labile Dissolved Organic Matter","authors":"Zachary A. Quinlan, Craig E. Nelson, Irina Koester, Daniel Petras, Louis-Felix Nothias, Jacqueline Comstock, Brandie M. White, Lihini I. Aluwihare, Barbara A. Bailey, Craig A. Carlson, Pieter C. Dorrestein, Andreas F. Haas, Linda Wegley Kelly","doi":"10.1111/1462-2920.70064","DOIUrl":"10.1111/1462-2920.70064","url":null,"abstract":"Dissolved organic matter (DOM) comprises diverse compounds with variable bioavailability across aquatic ecosystems. The sources and quantities of DOM can influence microbial growth and community structure with effects on biogeochemical processes. To investigate the chemodiversity of labile DOM in tropical reef waters, we tracked microbial utilisation of over 3000 untargeted mass spectrometry ion features exuded from two coral and three algal species. Roughly half of these features clustered into over 500 biologically labile spectral subnetworks annotated to diverse structural superclasses, including benzenoids, lipids, organic acids, heterocyclics and phenylpropanoids, comprising on average one-third of the ion richness and abundance within each chemical class. Distinct subsets of these labile compounds were exuded by algae and corals during the day and night, driving differential microbial growth and substrate utilisation. This study expands the chemical diversity of labile marine DOM with implications for carbon cycling in coastal environments.","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"27 3","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.70064","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143660635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0