Ecotoxicology最新文献

{"title":"Recommended rates of azoxystrobin and tebuconazole seem to be environmentally safe but ineffective against target fungi.","authors":"Libânia Queirós,&nbsp;Nuno Aguiar,&nbsp;Patrícia Pereira,&nbsp;Fernando J M Gonçalves,&nbsp;Artur Alves,&nbsp;Joana Luísa Pereira","doi":"10.1007/s10646-023-02619-w","DOIUrl":"https://doi.org/10.1007/s10646-023-02619-w","url":null,"abstract":"<p><p>The use of fungicides in agriculture has been playing a role in the enhancement of agricultural yields through the control of pathogens causing serious diseases in crops. Still, adverse environmental and human health effects resulting from its application have been reported. In this study, the possibility of readjusting the formulation of a commercial product combining azoxystrobin and tebuconazole (active ingredients - AIs; Custodia®) towards environmentally safer alternative(s) was investigated. Specifically, the sensitivity of non-target aquatic communities to each AI was first evaluated by applying the Species Sensitivity Distributions (SSDs) approach. Then, mixtures of these AIs were tested in a non-target organism (Raphidocelis subcapitata) denoting sensitivity to both AIs as assessed from SSDs. The resulting data supported the design of the last stage of this study, where mixtures of those AIs at equivalent vs. alternative ratios and rates as in the commercial formulation were tested against two target fungal species: Pyrenophora teres CBS 123929 and Rhynchosporium secalis CBS 110524. The comparison between the sensitivity of non-target aquatic species and the corresponding efficacy towards target fungi revealed that currently applied mixture and rates of these AIs are generally environmentally safe (antagonistic interaction; concentrations below the EC₁ for R. subcapitata and generally below the HC₅ for aquatic non-target communities), but ineffective against target organisms (maximum levels of inhibition of 70 and 50% in P. teres CBS 123929 and R. secalis CBS 110524, respectively). Results additionally suggest a potentiation of the effects of the AIs by the other formulants added to the commercial product at tested rates. Overall, this study corroborates that commercial products can be optimized during design stages based on a systematic ecotoxicological testing for ingredient interactions and actual efficacy against targets. This could be a valuable pathway to reduce environmental contamination during transition to a more sustainable agricultural production.</p>","PeriodicalId":11497,"journal":{"name":"Ecotoxicology","volume":"32 1","pages":"102-113"},"PeriodicalIF":2.7,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9883303/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10583379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Temporal dynamic of antibiotic resistance genes in the Zaohe-Weihe hyporheic zone: driven by oxygen and bacterial community.","authors":"Ruyi Feng,&nbsp;Lei Duan,&nbsp;Siqi Shen,&nbsp;Yan Cheng,&nbsp;Yanhua Wang,&nbsp;Wenke Wang,&nbsp;Shengke Yang","doi":"10.1007/s10646-022-02616-5","DOIUrl":"https://doi.org/10.1007/s10646-022-02616-5","url":null,"abstract":"<p><p>The widespread spread of antibiotic resistance genes (ARGs) in hyporheic zone (HZ) has become an emerging environmental problem due to their potentially harmful nature. In this research, three different oxygen treatment systems were set up to study the effects of oxygen changes on the abundance of ARGs in the HZ. In addition, the effects of temperature and salinity on ARGs were investigated under aerobic and anaerobic systems, respectively. The bacterial community composition of sediment samples and the relationship with ARGs were analyzed. The explanation ratio and causality of the driving factors affecting ARGs were analyzed using variation partitioning analysis (VPA) and structural equation model (SEM). The relative abundance of ARGs and mobile genetic elements (MGEs) in the anaerobic system increased significantly, which was higher than that in the aerobic system and the aerobic-anaerobic interaction system. The experiment of salinity and temperature also further proved this result. There were many bacterial communities that affected tetracycline and sulfonamide ARGs in sediments, and these host bacteria are mainly concentrated in Proteobacteria, Firmicutes and Bacteroidetes. VPA and SEM further revealed that the abundance of ARGs was mainly influenced by changes in bacterial communities and oxygen conditions, and horizontal gene transfer (HGT) of MGEs also had a positive effect on the spread of ARGs. Those findings suggest that complex oxygen conditions in the HZ alter bacterial communities and promote MGEs-mediated horizontal transfer, which together lead to the spread of ARGs. This study has value as a reference for formulating effective strategies to minimize the propagation of ARGs in underground environment.</p>","PeriodicalId":11497,"journal":{"name":"Ecotoxicology","volume":"32 1","pages":"57-72"},"PeriodicalIF":2.7,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10637638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toxicological signature for thyroid endocrine disruption of dichlorooctylisothiazolinone in zebrafish larvae.","authors":"Sujin Lee,&nbsp;Kyunghee Ji","doi":"10.1007/s10646-022-02614-7","DOIUrl":"https://doi.org/10.1007/s10646-022-02614-7","url":null,"abstract":"<p><p>Dichlorooctylisothiazolinone (DCOIT), which is one of the isothiazolinone preservatives, is applied to water-based adhesives in food packaging. This study investigated the effects of DCOIT on the embryonic growth and thyroid endocrine system using zebrafish. Organism-level (hatchability, survival, and growth), hormone-level (triiodothyronine (T3) and thyroxine (T4)), gene-level (genes associated with the hypothalamus-pituitary-thyroid axis), and microRNA-level (microRNAs related to thyroid endocrine disruption) endpoints were measured. Significant rise in embryonic coagulation and delayed hatching (≥0.3 μg/L), and decreased larval length (30 μg/L) were observed in fish exposed to DCOIT. Lower contents of T3 and T4 were observed after exposure to DCOIT, which was accompanied by the upregulation of genes associated with the thyrotropin releasing hormone and thyroid stimulating hormone and the downregulation of genes associated with the thyroid hormone receptors and deiodination. Strong influence of DCOIT on dre-miR-193b and -499 may be a critical mechanism to inhibit transcription of trαa and trβ, which in turn may affect thyroid hormones and development of the organism. Our findings suggest that hypothyroidism induced by the exposure to DCOIT is potentially associated with genetic and microRNA-level changes, which eventually affects development.</p>","PeriodicalId":11497,"journal":{"name":"Ecotoxicology","volume":"32 1","pages":"38-45"},"PeriodicalIF":2.7,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10659515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Roundup Original DI® and thermal stress affect survival, morphology and thermal tolerance in tadpoles of Boana faber (Hylidae, Anura).","authors":"Gabriela Alves-Ferreira,&nbsp;Marco Katzenberger,&nbsp;Fernanda Guimarães Fava,&nbsp;Renan Nunes Costa,&nbsp;Leildo Machado Carilo Filho,&nbsp;Mirco Solé","doi":"10.1007/s10646-023-02622-1","DOIUrl":"https://doi.org/10.1007/s10646-023-02622-1","url":null,"abstract":"<p><p>In amphibians, stressful environments can lead to accelerated metamorphosis at the expense of total length, resulting in the occurrence of morphological abnormalities. Many studies have linked the occurrence of these phenomena to the pollution of habitats by pesticides and thermal stress. Here, we assessed how exposure to Roundup Original DI® and higher constant temperatures affect the survival of Boana faber tadpoles and estimate the CL5096hs for the population. In addition, we evaluated how exposure to Roundup affects larval growth, morphology and thermal tolerance. Our findings suggest that even at sublethal doses, Roundup Original DI® may affect the survival of Boana faber larvae. There also appears to be an additive effect between Roundup and temperature increase on larval survival, however, we need to further explore this point to determine a pattern, proving to be a promising issue to be investigated in the future. We observed effects of chronic exposure to the herbicide formulation on the morphology and growth of the tadpoles, resulting in a reduction in total length and differences in the shape of the larvae. Although we did not recover any direct effects of herbicide exposure on CTMax, we did observe an upward trend in CTMax for tadpoles exposed to Roundup. Understanding how anthropogenic changes affect anuran persistence is fundamental for the management and conservation of the species and can be considered an initial step toward the formulation of legislations that regulate the use of herbicides.</p>","PeriodicalId":11497,"journal":{"name":"Ecotoxicology","volume":"32 1","pages":"93-101"},"PeriodicalIF":2.7,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10638973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physicochemical and bioinformatic characterization of Oreochromis niloticus vitellogenin as an endocrine disruption biomarker.","authors":"Tugstênio L Souza,&nbsp;Tobias P de Morais,&nbsp;Francisco Filipak Neto,&nbsp;Iracema Opuskevitch,&nbsp;Fernando C A S Ferreira,&nbsp;Marco Antônio Ferreira Randi,&nbsp;Ciro Alberto de Oliveira Ribeiro,&nbsp;Claudemir de Souza,&nbsp;Maritana Mela Prodocimo","doi":"10.1007/s10646-022-02612-9","DOIUrl":"https://doi.org/10.1007/s10646-022-02612-9","url":null,"abstract":"<p><p>Aquatic biota is increasingly being exposed to chemical pollutants due to human activities and the relationship between the level of environmental pollution and fish reproduction is a continuously ongoing issue. The vitellogenin (Vtg) protein synthesis can be induced in the liver of juvenile and male fish after stimulation of the estrogen receptor and therefore, Vtg has been used as a biomarker of xenoestrogen exposure in several fish species. The current study reported the first physicochemical characterization of Vtg from Oreochromis niloticus. Adult male fish were exposed to 17α-ethinylestradiol for Vtg induction. Purified vitellogenin from plasma showed low stability at 25 and 4 °C in saline conditions, and good stability in acidic (low pH) or in heated conditions. The 3D modeling provided useful information on the structure of O. niloticus Vtg showing conserved structural features. According to bioinformatics and experimental results, there are important structural differences between the two chemical forms of Vtg (VtgAb and VtgC) in a phylogenetic context. The present results add information about the development of ecotoxicological immunoassays to study the endocrine disruption in O. niloticus improving the Vtg performance as a biomarker of reproduction in fish.</p>","PeriodicalId":11497,"journal":{"name":"Ecotoxicology","volume":"32 1","pages":"12-24"},"PeriodicalIF":2.7,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10658782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Soil flooding and its outcome on cadmium and nutrient uptake affect photosynthetic activity in Inga laurina plants.","authors":"Namir G M Lopes,&nbsp;Rodrigo B Kloss,&nbsp;Ivanildes C Dos Santos,&nbsp;Vânia L Souza,&nbsp;Majeti N V Prasad,&nbsp;Pedro A O Mangabeira,&nbsp;Marcel G C França","doi":"10.1007/s10646-022-02615-6","DOIUrl":"https://doi.org/10.1007/s10646-022-02615-6","url":null,"abstract":"<p><p>Urban areas next to mangroves are subject to progressive heavy metal contamination. Treelets of Inga laurina were collected in this ecosystem and cultivated for 30 days in waterlogged conditions and closed pots (WC) and at field capacity (FC), while exposed to different Cd concentrations (0, 50 and 100 mg·kg^-1). Soil water conditions did not affect total Cd in plants, with Cd accumulating in roots and WC inducing less leaf chlorophyll while increasing carotenoids and chlorophyll ratio. Higher net photosynthesis, stomatal conductance, transpiration, and Ci/Ca ratio were observed under the highest Cd concentration and WC, while being conservative in water consumption as shown by the reduction in both water use efficiencies. Nutritional uptake behaved differently for each element, with N, Mg and Ca not being affected by Cd under WC but K increasing with Cd. At FC, plants showed higher values than WC, with the highest Cd concentration at FC showing the highest values overall. Nutrient allocation in organs was affected by WC in N, Mg, K, P and Ca but only P by Cd, with WC reducing nutrients overall and N, Mg and Ca behaving the same in both soil conditions while K was lowered in leaves and increased in roots under WC. P allocation under WC was not hindered by Cd even showing higher values in it than FC in some treatments. Altogether, results indicate that I. laurina can be considered a Cd-tolerant species, especially in WC and it presents a potential to be used as a phytoremediator plant.</p>","PeriodicalId":11497,"journal":{"name":"Ecotoxicology","volume":"32 1","pages":"73-81"},"PeriodicalIF":2.7,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10639340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of temperature and soil moisture on the toxic potential of clothianidin to collembolan Folsomia candida in a tropical field soil.","authors":"Thalia Smaniotto Graciani,&nbsp;Felipe Ogliari Bandeira,&nbsp;Elke Jurandy Bran Nogueira Cardoso,&nbsp;Paulo Roger Lopes Alves","doi":"10.1007/s10646-023-02621-2","DOIUrl":"https://doi.org/10.1007/s10646-023-02621-2","url":null,"abstract":"<p><p>Climate change can alter the toxic effects of pesticides on soil invertebrates. However, the nature and magnitude of the influence of climatic factors on clothianidin impacts in tropical soils are still unknown. The influence of increasing atmospheric temperature and the reduction in soil moisture on the toxicity and risk of clothianidin (seed dressing formulation Inside FS^®) were assessed through chronic toxicity tests with collembolans Folsomia candida in a tropical field soil (Entisol). The risk of clothianidin for collembolans was estimated using the Toxicity-Exposure Ratio (TER) approach. Organisms were exposed to increasing clothianidin concentrations at 20, 25 and 27 °C in combination with two soil moisture conditions (30 and 60% of the maximum water holding capacity-WHC). The effect of temperature and soil moisture content on clothianidin toxicity was verified through the number of F. candida juveniles generated after 28 days of exposure to the spiked soil. The toxicities estimated at 25 °C (EC₅₀__30%WHC = 0.014 mg kg^-1; EC_{50_60%WHC} = 0.010 mg kg^-1) and 27 °C (EC_{50_30%WHC} = 0.006 mg kg^-1; EC_{50_60%WHC} = 0.007 mg kg^-1) were 2.9-3.0-fold (25 °C) and 4.3-6.7-fold (27 °C) higher than those found at 20 °C (EC_{50_30%WHC} = 0.040 mg kg^-1; EC_{50_60%WHC} = 0.030 mg kg^-1), indicating that clothianidin toxicity increases with temperature. No clear influence of soil moisture content on clothianidin toxicity could be observed once the EC₅₀ values estimated at 30% and 60% WHC, within the same temperature, did not significantly differ. A significant risk was detected in all temperatures and soil moisture scenarios studied, and the TER values indicate that the risk can increase with increasing temperatures. Our results revealed that temperature could overlap with soil moisture in regulating clothianidin toxicity and reinforce the importance of including climatic factors in the prospective risk assessment of pesticides.</p>","PeriodicalId":11497,"journal":{"name":"Ecotoxicology","volume":"32 1","pages":"82-92"},"PeriodicalIF":2.7,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10638207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biotransformation and detoxification mechanism of inorganic arsenic in a freshwater benthic fish Tachysurus fulvidraco with dietborne exposure.","authors":"Denglong Lu,&nbsp;Wenbao Luo,&nbsp;Haipu Li,&nbsp;Zhaoguang Yang","doi":"10.1007/s10646-022-02611-w","DOIUrl":"https://doi.org/10.1007/s10646-022-02611-w","url":null,"abstract":"<p><p>Inorganic arsenic (iAs) is a pervasive environmental toxin, its metabolism and detoxification mechanism in freshwater benthic fish under dietary exposure remain unknown. In this study, dietborne exposure of two iAs (arsenate (AsV) and arsenite (AsIII)) was conducted for yellow catfish (Tachysurus fulvidraco) to investigate the bioaccumulation, biotransformation, and detoxification of iAs in the fish liver. The results showed that As significantly accumulated in both the AsIII and AsV treatments compared to the control, and the final As concentration was comparable for both treatments. The detoxification of iAs in freshwater fish depends on the degree of arsenic methylation and the level of antioxidants. Both reduction processes of AsV to AsIII and oxidation processes of AsIII to AsV were found in AsV and AsIII treatments. The major-low toxicity intermediates, which also are detoxification products in the AsIII treatment, were pentavalent dimethylarsinic acid and arsenobetaine (AsB), and AsB was major-low toxicity intermediate in the AsV treatment. Both antioxidants glutathione and glutathione S-transferase contribute to the detoxification of iAs by scavenging excessive reactive oxygen species and promoting iAs methylation in yellow catfish under iAs exposure.</p>","PeriodicalId":11497,"journal":{"name":"Ecotoxicology","volume":"32 1","pages":"46-56"},"PeriodicalIF":2.7,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9204855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to: Assessing the potential chronic, sublethal and lethal ecotoxicity of land-applying biosolids on Folsomia candida and Lumbricus terrestris.","authors":"Karen J Puddephatt,&nbsp;Lynda H McCarthy,&nbsp;Bryant M Serre","doi":"10.1007/s10646-022-02618-3","DOIUrl":"https://doi.org/10.1007/s10646-022-02618-3","url":null,"abstract":"","PeriodicalId":11497,"journal":{"name":"Ecotoxicology","volume":"32 1","pages":"135"},"PeriodicalIF":2.7,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9188134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Embryonic toxicity of Imidacloprid: Impact on hatchability, survivability, swimming speed and cardiac function of catfish, Clarias gariepinus.","authors":"Nosakhare Osazee Erhunmwunse,&nbsp;Isioma Tongo,&nbsp;Kelvin Omigie","doi":"10.1007/s10646-023-02625-y","DOIUrl":"https://doi.org/10.1007/s10646-023-02625-y","url":null,"abstract":"<p><p>Imidacloprid is a systemic insecticide that belongs to the neonicotinoid class of chemicals that act on the central nervous system of insects. Imidacloprid is used to control sucking insects, chewing insects such as termites, soil insects, and fleas on pets, as well as to treat structures, crops, soil, and seeds. As a result of these factors, this pesticide may end up in the aquatic environment via municipal discharges and runoff. Although the presence of imidacloprid in aquatic environments has been underreported as widespread, the toxic effects of this pesticide may have serious implications on aquatic organisms, particularly at environmentally relevant concentrations and demand more attention. Given this knowledge, the goal of this study was to investigate the effects of imidacloprid on Clarias gariepinus embryonic development. Clarias gariepinus embryos (3 h post-fertilization) were exposed to environmentally relevant concentrations of imidacloprid (10, 30, 100, and 500 µg/L) until 48 h post-fertilization using a modified fish embryo acute toxicity test (OECD TG 236). A stereomicroscope was used to assess hatchability, deformity, heart rate, and swimming speed as endpoints. According to our results of the developmental acute toxicity test, imidacloprid significantly reduced the hatching rate and heartbeats of C. gariepinus embryos. It also influenced the swimming kinematics of exposed embryos and caused teratogenic effects such as yolk sac rupture, pericardial oedema, lordosis, an abnormally shaped head, and altered epiboly. Our results allow us to conclude that imidacloprid is a toxic pesticide in the early life stages of C. gariepinus due to its high teratogenic potential.</p>","PeriodicalId":11497,"journal":{"name":"Ecotoxicology","volume":"32 1","pages":"127-134"},"PeriodicalIF":2.7,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10584141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}