Ecotoxicology最新文献

{"title":"Impact of field evolved resistance on biological parameters of non-targeted Aedes aegypti populations.","authors":"Nimra Batool, Muhammad Asif Farooq, Waqar Jaleel, Ahmed Noureldeen, Akram Alghamdi, Hadeer Darwish, Naif H Ashri, Muhammad Nadir Naqqash","doi":"10.1007/s10646-024-02842-z","DOIUrl":"https://doi.org/10.1007/s10646-024-02842-z","url":null,"abstract":"<p><p>The yellow fever mosquito, Aedes aegypti L., known for transmitting viruses causing yellow fever, dengue, chikungunya, and Zika fever, presents a substantial risk to global human health. The development of insecticide resistance in disease vectors has become a significant problem in Ae. aegypti. Monitoring insecticide resistance is essential for resistance management in Ae. aegypti. This study involved the collection of Ae. aegypti populations from four important cotton-growing regions in southern Punjab, Pakistan, for resistance monitoring over a two-year period (2021-2022). This study also assessed the impact of insecticide resistance on biological parameters of Ae. aegypti. Moderate-to-high levels of resistance were observed against all the tested insecticides viz., chlorpyrifos, chlorfenapyr, deltamethrin, flonicamid, spirotetramat, and spinetoram. However, compared to the Lab-susceptible population, higher levels of resistance to buprofezin (59.03-84.40) and imidacloprid (68.49-100.01) were found in all populations. This high resistance can be attributed to increased use of these two insecticides in cotton fields, as compared to other insecticides. In the lab-susceptible population, higher values for the intrinsic rate of increase (r) and the net reproductive rate (R₀) i.e., 0.20 per day and 23.24 offspring/female were observed, respectively. This was also validated by population projection data where more than 2.5-fold adults (1,020,361.80 individuals) were calculated in the Lab-susceptible population as compared to the most resistant populations. Sublethal exposure to insecticides may induce physiological or biochemical changes in organisms, subsequently influencing the biological traits. Resistance monitoring provides essential guidance before launching a successful chemical-based vector management program.</p>","PeriodicalId":11497,"journal":{"name":"Ecotoxicology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142817313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of enzymatic and non-enzymatic biomarkers of sublethal cadmium toxicity in the freshwater mussel (Unio tigridis).","authors":"Esin G Canli, Mustafa Canli","doi":"10.1007/s10646-024-02844-x","DOIUrl":"https://doi.org/10.1007/s10646-024-02844-x","url":null,"abstract":"<p><p>Mussels are filter-feeding animals with a sedentary lifestyle and thus, they were accepted as good bioindicator animals to investigate environmental pollution. In this study, freshwater mussels (Unio tigridis) were exposed to cadmium (0, 30, 90, 270 µg Cd/L) for up to 21 days. Then, the responses of several biomarkers belonging to the antioxidant, osmoregulation and nervous systems, as well as the energy reserves of mussels were investigated. The animals were fed on laboratory-cultured algae (Chlorella vulgaris) during the experiments. Data showed that the exposure conditions did not cause mussel mortality within 21 days, though the levels of all biomarkers altered significantly (p < 0.05) compared to controls. Cadmium exposures significantly altered the activities of antioxidant enzymes in the digestive glands. Similarly, malondialdehyde (MDA) levels in the digestive glands significantly increased after cadmium exposures. Likewise, acetylcholinesterase (AChE) activity and Ca-ATPase activity in the muscle significantly decreased. There were decreases in Na-ATPase and increases in Mg-ATPase activities in the gill. The total energy reserves of mussels significantly decreased, especially at the higher cadmium concentrations. This study showed that environmentally relevant cadmium concentrations could alter the levels of biomarkers belonging to different metabolic systems, emphasizing their possible usage in evaluating metal contamination.</p>","PeriodicalId":11497,"journal":{"name":"Ecotoxicology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142791260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hidden target, hidden effects: chlorantraniliprole on the coffee leaf miner (Leucoptera coffeella).","authors":"D S Souza, C A D Nascimento, J Broêtto, M T C S Resende, L S Carneiro, M A Castellani, J E Serrão, R N C Guedes","doi":"10.1007/s10646-024-02845-w","DOIUrl":"https://doi.org/10.1007/s10646-024-02845-w","url":null,"abstract":"<p><p>Agricultural insecticide usage presents a complex challenge, particularly when addressing hidden targets such as concealed pest species. Typically, insecticide spraying targets either the host plant or the soil substrate, reaching the target when the pests move or feed, yet their vulnerability when concealed remains low. This study delves into the often-obscure effects of insecticides on hidden herbivore species, focusing specifically on the diamide insecticide chlorantraniliprole and its impact on the concealed insect herbivore, the coffee leaf miner Leucoptera coffeella (Guérin-Mèneville & Perrottet) (Lepidoptera: Lyonetidae). We document the progressive effects of chlorantraniliprole from egg-laying through the insect's development and reproductive output. By examining egg-laying preferences, development, survival, respiration rate, leaf consumption, and polysaccharide and protein accumulation, alongside fecundity, in two field-collected leaf miner populations, we elucidate chlorantraniliprole's broader effects. While the insecticide did not alter the leaf miner's egg-laying preferences, one population exhibited higher larval survival, indicating chlorantraniliprole resistance. This chlorantraniliprole-resistant population displayed a lower respiration rate-indicative of reduced stress-and higher leaf consumption, accompanied by increased sugar and protein accumulation. Although this population showed lower adult longevity, it exhibited higher fecundity. These findings highlight the multifaceted impacts of insecticides, extending beyond survival to affect development, fecundity, and potential fitness. Variations in response among insect populations suggest that resistant insects may outperform susceptible ones even under sublethal exposure, with significant implications for management strategies and future outbreaks.</p>","PeriodicalId":11497,"journal":{"name":"Ecotoxicology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142791265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ecotoxicological evaluation of urban wastewater treatment plants: a Sicilian study.","authors":"Paola Rapisarda, Gea Oliveri Conti, Eloise Pulvirenti, Antonio Cristaldi, Claudia Favara, Maria Fiore, Chiara Copat, Alfina Grasso, Maria Castrogiovanni, Giuseppe Mancini, Margherita Ferrante","doi":"10.1007/s10646-024-02840-1","DOIUrl":"https://doi.org/10.1007/s10646-024-02840-1","url":null,"abstract":"<p><p>(1) Background: Ecotoxicological screening evaluates the acute toxicity of WWs. The Vibrio fischeri ecotoxicological assay analyses inlet and outlet wastewater samples from two urban wastewater treatment plants in Catania, Sicily, Italy. (2) Methods: The APAT CNR IRSA 8030 Man 29 method was used as method; (3) Results: The results showed toxicity values below the limit of the Italian Legislative Decree 152/06; (4) Conclusions: This monitoring study allows to verify the efficacy, and the outlet quality of WWs discharged to sea water. This ecotoxicological assay is a valuable tool for evaluating the combined toxicity of various pollutants that underline the total damage of the studied matrices detecting the true effect of complex mixtures on the environment and its fauna.</p>","PeriodicalId":11497,"journal":{"name":"Ecotoxicology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142779661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ecological risk assessment of p-toluidine in freshwater, sediment, and soil media.","authors":"Inhye Lee, Daeyeop Lee, Hun-Je Jo, Hyun Soo Kim, Kyung Sook Woo, Kyunghee Ji","doi":"10.1007/s10646-024-02834-z","DOIUrl":"https://doi.org/10.1007/s10646-024-02834-z","url":null,"abstract":"<p><p>Para-toluidine (p-toluidine) is a high production volume chemical, of which about 5,000 tons/year are imported into Korea as of the 2018 registration. This substance is mainly used as an intermediate in the manufacture of paints, azo dyes, and pigments. In this study, an ecological risk assessment of p-toluidine was performed to investigate the risk to the environment assuming the worst-case exposure scenario. Ecotoxicity data was collected from the literature and the predicted no effect concentration (PNEC) was derived. The predicted environmental concentration (PEC) was calculated using the SimpleBox Korea v2.0 model. Hazard quotient (HQ) was calculated based on PEC and PNEC. The concentration of p-toluidine was measured in soil samples from workplaces with a HQ exceeding 1 to verify the results of modeling. PNECs for surface water, sediment, and soil were 0.00022 mg/L, 0.0025 mg/kg (dw), and 0.00037 mg/kg (dw), respectively. Based on regional-scale PEC, the HQ of freshwater, sediment, and soil media were all less than 1, but the HQ exceeded 1 at some local sites. Based on the fact that the measured environmental concentration in soil samples around the workplace is below the limit of detection and that all waste is incinerated at the actual workplace, p-toluidine is not likely to pose a high risk to the environment in Korea.</p>","PeriodicalId":11497,"journal":{"name":"Ecotoxicology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142767459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating the toxicity of polylactic acid microplastics on the health and physiology of freshwater fish, Cirrhinus mrigala.","authors":"Eram Rashid, Syed Makhdoom Hussain, Shafaqat Ali, Pallab K Sarker, Mohammad Abul Farah","doi":"10.1007/s10646-024-02813-4","DOIUrl":"10.1007/s10646-024-02813-4","url":null,"abstract":"<p><p>The widespread presence of microplastics (MPs) in aquatic ecosystems has raised growing concerns among ecotoxicologists regarding their potential toxicity. This study explored the impacts of polylactic acid (PLA) MPs on the physiology and health of freshwater fish, Cirrhinus mrigala, by dietary exposure for 90 days. The experiment consisted of six groups: five treatment groups (0.5%, 1%, 1.5%, 2%, and 2.5% PLA-MP) and a control group (0% PLA-MP). Each group was comprised of fifteen fish, and the experiment was replicated three times. The exposure severity of PLA-MPs varied from low to high, with treatment levels ranging from 0.5% to 2.5% PLA-MPs, relative to the control group. This exposure significantly affected their growth performance. Additionally, the apparent digestibility of the SFM-based diet decreased with increasing PLA-MPs concentration. Exposure to PLA-MPs induced considerable changes in body composition, characterized by increased moisture and crude fat content and decreased ash content and crude protein. The blood profile, including MCHC, RBCs, Hb, PLT and PCV exhibited significant declines in the high treatment group (2.5% PLA-MPs), while MCH, WBCs and MCV showed notable increases. Furthermore, histopathological examination of the intestine revealed an increase in abnormalities in the intestine at 2.5% PLA-MPs level. The high treatment group (2.5% PLA-MPs) showed the lowest mineral content in the fish muscles. In summary, dietary exposure to PLA-MPs led to alterations in overall body performance across the treatment groups, ranging from low to high severity levels.</p>","PeriodicalId":11497,"journal":{"name":"Ecotoxicology","volume":" ","pages":"1210-1221"},"PeriodicalIF":2.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142361328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toxicodynamics of cadmium in the green mussel Perna viridis (Linnaeus, 1758) using bioenergetic and physiological biomarkers.","authors":"Kariyil Veettil Neethu, Neethu Xavier, Punnakkal Hari Praved, Naduvath Deepak Sankar, Punnathi Anilkumar Athira, Sivasankaran Bijoy Nandan, Kandussery Joseph Joseph, Shambanagouda Rudragouda Marigoudar, Krishna Venkatarama Sharma","doi":"10.1007/s10646-024-02814-3","DOIUrl":"10.1007/s10646-024-02814-3","url":null,"abstract":"<p><p>This study evaluated the toxicity of cadmium (Cd) on the green mussel Perna viridis, aiming to identify toxicological endpoints and investigate its responses across physiological, bioenergetic, and biochemical parameters. The 96-hour LC₅₀ value for Cd in P. viridis was 3.03 ± 0.12 mg L^-1, with a 95% confidence interval of 2.35-3.91 mg L^-1. Chronic toxicity tests revealed a No Observable Effect Concentration (NOEC), Lowest Observable Effect Concentration (LOEC), and chronic toxicity values of 0.20, 0.37, and 0.29 mg L^-1, respectively. Cadmium accumulation in treated mussels increased 46- to 215-fold compared to the control group. Superoxide dismutase, catalase, glutathione S-transferase, and glutathione peroxidase levels in exposed mussels exhibited a significant increase compared to the control group. The redox index ratio, acetylcholinesterase activity, and lysosomal membrane stability decreased with increasing exposure concentrations. Levels of reduced and oxidized glutathione, glutathione reductase, lipid peroxidation, and metallothionein-like proteins increased in exposed mussels. Clearance rate, respiration rate, and excretion rate decreased in a dose-dependent manner. Protein, carbohydrate, and lipid levels decreased with increasing exposure concentration (p < 0.001). Mitochondrial electron transport system activity increased, while cellular energy allocation (p < 0.001) and scope for growth decreased in a dose-dependent manner (p < 0.01). The significant increase in antioxidants suggests heightened oxidative stress in mussels under Cd exposure. The physiological activities of the mussels were severely affected, ultimately leading to a reduced scope for growth. The toxicological data generated in this study contribute to the development of seawater quality criteria for the metal Cd.</p>","PeriodicalId":11497,"journal":{"name":"Ecotoxicology","volume":" ","pages":"1222-1241"},"PeriodicalIF":2.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142388928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of aerial liming on soil chemical and biological properties in metal contaminated and inaccessible lands in Ontario (Canada).","authors":"Katheryn Klajman, Peter Beckett, Graeme Spiers, Kabwe Nkongolo","doi":"10.1007/s10646-024-02804-5","DOIUrl":"10.1007/s10646-024-02804-5","url":null,"abstract":"<p><p>The manual addition of lime to soil, in addition to tree planting and fertilization have been the dominant strategy described in restoration protocols for ecosystems damaged by acid rain and metal contamination. Investigations on aerial-limed soils in inaccessible lands are limited. The objective of this study was to assess the effects of aerial liming on soil pH, organic matter, microbial biomass, and enzymatic activities, and aboveground plant population quality in metal-contaminated lands in northern Ontario, Canada. Soil samples were collected from three sites around the City of Greater Sudbury with each pair being composed of a reclaimed (areal-limed) site and an adjacent undisturbed (unlimed) area. Soil physico-chemistry, microbial biomass (assessed by Phospholipid fatty acid analysis) and enzymatic activities were analyzed. Soil pH was higher in limed sites compared to unlimed at recently restored sites (Baby Lake and Wahnapitae) but not at the oldest reclaimed site (HWY 80 N). Organic matter was higher in limed areas compared to the unlimed reference site only at most recently reclaimed site at Baby Lake. Aboveground plant population health was visibly improved in limed sites compared to unlimed areas. Metal concentrations of iron (Fe) and arsenic (As), total microbial biomasses, gram-negative bacterial, fungal, and eukaryotic biomasses were all significantly increased in the limed soils compared to the unlimed samples. The same trend was observed for the activities of three of the enzymes tested, β-N-acetylglucosaminidase (BG), aryl sulfatase (AS), and glycine aminopeptidase (GAP). Interestingly, strong positive correlations between the levels of soil organic matter, microbial biomasses, and NAGase and ALP activities were observed. Although expensive, aerial liming is effective in restoring inaccessible sites impacted by smelting operations where other methods cannot easily be used.</p>","PeriodicalId":11497,"journal":{"name":"Ecotoxicology","volume":" ","pages":"1145-1160"},"PeriodicalIF":2.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142379224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytotoxicity of single and mixed rare earth element (La, Nd and Sm) exposures on Lactuca sativa seed germination and growth.","authors":"Silvia Gonçalves Egler, Tamine Martins Roldão, Gabriel Oliveira Santos, Gisele Petronilho Heidelmann, Inês Gomes Fraga, Fabio Veríssimo Correia, Enrico Mendes Saggioro","doi":"10.1007/s10646-024-02812-5","DOIUrl":"10.1007/s10646-024-02812-5","url":null,"abstract":"<p><p>The mode of action, bioaccumulation potential and toxicity of Rare Earth Elements (REE), with several applications in the technology, medical and agricultural fields, are still understudied. The nitrates acute effects on single exposures, binary and ternary mixtures of lanthanum (La), neodymium (Nd) and samarium (Sm) on Lactuca sativa lettuce seed germination and wet biomass in an artificial soil (AS) and an Ultisol were assessed. Germination (EC₅₀), wet biomass (IC₅₀) and germination inhibition (% GI) were evaluated. EC₅₀ values show La was the most toxic in Ultisol, Sm in AS, and Nd appears with intermediate values on both substrates. The IC₅₀, both single and mixed, decreased from 3- to 181-fold with increasing test concentrations in relation to the control in AS, while increases in Ultisol were observed, followed by decreases at higher doses which may be associated with the low-dose stimulation effect (hormesis). Our findings may be used to subsidize REE risk assessment studies and reinforce the hormesis effect to prevent the use of high application of REE fertilizers, avoiding the accumulation of REE in agricultural soils.</p>","PeriodicalId":11497,"journal":{"name":"Ecotoxicology","volume":" ","pages":"1193-1209"},"PeriodicalIF":2.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142388926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stress biomarker response in Aporrectodea caliginosa earthworms exposed to single and combined pesticide treatments (Prosaro and Decis).","authors":"Djamila Hafsi, Ibtissem Sbartai, Hana Sbartai","doi":"10.1007/s10646-024-02811-6","DOIUrl":"10.1007/s10646-024-02811-6","url":null,"abstract":"<p><p>This study aims to assess the impact of two pesticides commonly used in Algeria (Prosaro XRT and Decis 25 EC), as well as their combinations at recommended doses, on a non-target species bioindicator of soil pollution, the earthworm Aporrectodea caliginosa, using physiological (mortality and growth) and biochemical parameters (proteins, glutathione, catalase activity and glutathione S-transferase, acetylcholine esterase, lipoxygenase). The recommended dose and its double were tested individually and in combination for this. It should be noted that the protocol used and the initial concentrations selected are the same as those used in the field. After 7 and 14 days (7D/14D) of exposure, all dosages were administered. Our findings show that the pesticides tested had no effect on earthworm survival. However, a significant decrease in their growth rates depending on the different concentrations was observed for the different treatments over the entire exposure period of 7 or 14 D. The greatest reductions (31.62%, 35.04%) are reported after 14D for the high concentrations of Decis alone (D2) as well as for the combined treatment Prosaro/Decis (P2/D2). At the same time, an increase in total protein contents (more than 50% after 14D) as well as a decrease in acetylcholine esterase activity were reported for all treatments. We were also able to identify the induction of oxidative stress after xenobiotic exposure, which is more pronounced at the end of the treatment (14D), resulting in the stimulation of the antioxidant system (gluthione, glutathione S-transférase, catalase) as well as the induction of lipoxygenase, which is responsible for the oxidation of polyunsaturated fatty acids as well as the generation of reactive oxygen species (ROS) involved in the inflammatory phenomenon. Finally, it turns out that the species Aporrectodea caliginosa is sensitive to the different concentrations applied, even those used in the open field, and that Decis (deltamethrin) seems to be more toxic than Prosaro and that the combinaison P2/D2 is as toxic as Decis alone (D2).</p>","PeriodicalId":11497,"journal":{"name":"Ecotoxicology","volume":" ","pages":"1180-1192"},"PeriodicalIF":2.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142388927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}