EcotoxicologyPub Date : 2024-12-01DOI: 10.1007/s10646-024-02841-0
José Ângeles Moreira de Oliveira, Leonardo David Tuffi Santos, Murilo Antônio Oliveira Ruas, Luan Mateus Silva Donato, Victor Augustus Vasconcelos de Oliveira, Yuri Silva Saraiva Guimarães, Richardson Fernandes de Souza, Rodinei Facco Pegoraro
{"title":"Picloram drift in Peltophorum dubium, a species native to the Brazilian Cerrado.","authors":"José Ângeles Moreira de Oliveira, Leonardo David Tuffi Santos, Murilo Antônio Oliveira Ruas, Luan Mateus Silva Donato, Victor Augustus Vasconcelos de Oliveira, Yuri Silva Saraiva Guimarães, Richardson Fernandes de Souza, Rodinei Facco Pegoraro","doi":"10.1007/s10646-024-02841-0","DOIUrl":"https://doi.org/10.1007/s10646-024-02841-0","url":null,"abstract":"<p><p>Peltophorum dubium, a common tree in areas close to agricultural activity in the Brazilian Cerrado, is vulnerable to damage from the drift of picloram, an herbicide widely used in pastures and agriculture in Brazil. The aim was to evaluate the application of 0.0; 19.2; 38.4; 76.8; 153.6; 307.2 g e. a. ha<sup>-1</sup> of picloram, in simulated drift, on P. dubium, as well as its use as a bioindicator plant for herbicide contamination. The doses of picloram applied to the plants caused symptoms typical of the action of picloram on sensitive plants and led to the death of P. dubium at the highest doses tested. At its highest dose, picloram caused a 52.86% reduction in photosynthetic rate, 42.51% in transpiration rate, and 64.28% in stomatal conductance compared to the non-treated control. Picloram at a dose of 19.2 g e. a. ha<sup>-1</sup> caused a reduction in N content and utilization and reduced plant protein. Picloram drift causes severe damage to P. dubium, reinforcing the concern about the risks of contamination with the herbicide. The species acted as a bioindicator of picloram in the environment and could be used in biomonitoring herbicide contamination.</p>","PeriodicalId":11497,"journal":{"name":"Ecotoxicology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142767504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
EcotoxicologyPub Date : 2024-11-30DOI: 10.1007/s10646-024-02837-w
María Timaná Morales, Viridiana Peraza Gómez, Eva R Kozak, José Vladimir Trejo Flores, Maricruz Robles Ravelero, Luis Daniel Espinosa Chaurand, Edgar Iván Jiménez Ruíz
{"title":"Microplastics in marine fish: a mini-review on presence, classification, and impacts.","authors":"María Timaná Morales, Viridiana Peraza Gómez, Eva R Kozak, José Vladimir Trejo Flores, Maricruz Robles Ravelero, Luis Daniel Espinosa Chaurand, Edgar Iván Jiménez Ruíz","doi":"10.1007/s10646-024-02837-w","DOIUrl":"https://doi.org/10.1007/s10646-024-02837-w","url":null,"abstract":"<p><p>Plastic production has experienced exponential growth in recent years due to its diverse industrial applications, low cost, and high availability, also causing issues, since plastic waste in aquatic ecosystems transforms into microplastics (MPs) through mechanical and weathering processes. Microplastics are distributed ubiquitously in water bodies, where they can be ingested by a wide aquatic organism range, including fish, which have been used as bioindicators to assess microplastic presence and toxicity. Research has revealed microplastic presence in various fish species worldwide; the most common characteristics are fibers and fragments of blue, black, and transparent colors, and polyethylene, terephthalate, polypropylene and cellophane chemical composition. Experimental studies under laboratory conditions have demonstrated microplastics impact on fish, showing physical, immunological, and hematological damage, and oxidative stress ultimately leading to organisms' death. However, laboratory results do not necessarily predict impacts on wild fish due to different conditions to which the organisms are exposed. Therefore, further research needs to simulate real scenarios faced by wild fish in the marine environment, providing greater certainty about microplastic impacts and negative effects.</p>","PeriodicalId":11497,"journal":{"name":"Ecotoxicology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142767470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
EcotoxicologyPub Date : 2024-11-29DOI: 10.1007/s10646-024-02836-x
Liu Yang, Kexin Liu, Lina Shi, Ming Chen, Junyan Liu, Shiniu Dai, Yilong Xi, Xinli Wen
{"title":"Chronic toxicity and intergenerational effects of N-(1,3-dimethylbutyl)-N'-phenyl-p-phenylenediamine (6PPD) exposure alone and in combination with Zn<sup>2+</sup> on Daphnia magna (Cladocera).","authors":"Liu Yang, Kexin Liu, Lina Shi, Ming Chen, Junyan Liu, Shiniu Dai, Yilong Xi, Xinli Wen","doi":"10.1007/s10646-024-02836-x","DOIUrl":"https://doi.org/10.1007/s10646-024-02836-x","url":null,"abstract":"<p><p>N-(1,3-dimethylbutyl)-N'-phenyl-p-phenylenediamine (6PPD) and Zn<sup>2+</sup>, extensively used in the tire manufacturing process, are frequently detected in freshwater environments. However, the intergenerational effects of isolated 6PPD exposure and joint 6PPD and Zn<sup>2+</sup> exposure at concentrations approximating environmental levels remain unknown. This study assessed the chronic toxicity and intergenerational effects of 6PPD (0.02-20 μg/L) and a mixture of 6PPD and Zn<sup>2+</sup> (5 μg/L) over three generations in Daphnia magna bioassays. In the F0 generation, a dose-dependent decline in total offspring number was observed with 6PPD exposure alone, while co-exposure with Zn<sup>2+</sup> exacerbated the reproductive toxicity of 6PPD. Across three generations, low-dose (0.02 µg/L) 6PPD alone and combined with Zn<sup>2+</sup> induced a cumulative degenerative maternal effect. Conversely, high-dose (20 µg/L) 6PPD, both independently and in combination with Zn<sup>2+</sup>, exhibited an adaptive maternal effect. Notably, the grandmaternal effect emerged exclusively in the co-exposure group treated with 20 μg/L 6PPD and 5 μg/L Zn<sup>2+</sup>, with no such effect in the group exposed to 20 μg/L 6PPD alone, suggesting that Zn<sup>2+</sup> may enhance the potential toxicity of 6PPD. Overall, this study provides novel insight into the intergenerational impacts of environmentally relevant levels of 6PPD alone and in combination with a heavy metal, elucidating the environmental risks posed by tire-derived chemicals through their synergistic effects on transgenerational toxicity.</p>","PeriodicalId":11497,"journal":{"name":"Ecotoxicology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142750480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
EcotoxicologyPub Date : 2024-11-29DOI: 10.1007/s10646-024-02839-8
Maria Eduarda Maia, Rafael Xavier Martins, Matheus Carvalho, Luís M Félix, Luis Fernando Marques-Santos, Davi Farias
{"title":"Effects of atrazine, diuron and glyphosate mixtures on zebrafish embryos: acute toxicity and oxidative stress responses.","authors":"Maria Eduarda Maia, Rafael Xavier Martins, Matheus Carvalho, Luís M Félix, Luis Fernando Marques-Santos, Davi Farias","doi":"10.1007/s10646-024-02839-8","DOIUrl":"https://doi.org/10.1007/s10646-024-02839-8","url":null,"abstract":"<p><p>Synthetic pesticides are known for their toxic effects on non-target aquatic organisms. However, little is known about their effects when present in mixtures, which are closer to realistic exposure scenarios. Therefore, this study evaluates the toxicity of pesticides such as diuron, atrazine and glyphosate, individually and in combination, in zebrafish embryos, investigating their mechanisms of oxidative stress. The results revealed acute toxicity for diuron and atrazine, with LC<sub>50</sub> values of 9.6 mg/L and 53.57 mg/L for 96-h-old zebrafish, respectively. On the other hand, no effect was observed for glyphosate alone at the maximum concentration tested (100 mg/L). The mixture of diuron and atrazine showed a synergistic effect, resulting in a decrease in the LC<sub>50</sub> of each pesticide. Mixtures of diuron + glyphosate and atrazine + glyphosate were considered additive and antagonistic, respectively. All biomarkers analyzed (AChE, LDH, GST, CAT and GPx) showed significant changes. Furthermore, an increase in ROS production was observed in larvae exposed to individual and in the mixture composed of atrazine and diuron. These findings indicate that atrazine and diuron exhibit increased toxicity when combined, with their mechanisms of action-both in isolation and in mixtures-being at least partially linked to oxidative stress.</p>","PeriodicalId":11497,"journal":{"name":"Ecotoxicology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142750481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
EcotoxicologyPub Date : 2024-11-29DOI: 10.1007/s10646-024-02838-9
Rachel Raymer, Soraya M Jessa, W James Cooper, M Brady Olson
{"title":"The effects of diatom polyunsaturated aldehydes on embryonic and larval zebrafish (Danio rerio).","authors":"Rachel Raymer, Soraya M Jessa, W James Cooper, M Brady Olson","doi":"10.1007/s10646-024-02838-9","DOIUrl":"https://doi.org/10.1007/s10646-024-02838-9","url":null,"abstract":"<p><p>Marine diatoms are pervasive in many planktonic and benthic environments and represent an important food source for a wide range of species. Some diatoms produce polyunsaturated aldehydes (PUAs) as defensive toxins. PUA exposure is known to reduce the fecundity of invertebrate grazers like copepods and echinoderm larvae, but little is known about the effects of PUAs on vertebrates. Many fish species are likely to come into close contact with diatoms. Many may deposit eggs on diatom-coated substrates, consume diatoms as larvae, and/or feed heavily on zooplankters that may be gut-loaded with diatoms. The purpose of this study was to test whether dissolved diatom PUAs affect the early life stages of a model fish, Danio rerio (zebrafish). To test this, zebrafish embryos and larvae were exposed to proportionally increasing mixtures of the dissolved diatom PUAs 2E,4E-decadienal, 2E,4E-octadienal and 2E,4E-heptadienal. Under PUA exposure, three metrics of fitness were assessed: embryo heart rate, larval size at hatch, and pre-feeding mortality rate. Zebrafish embryos exposed at 24 h post fertilization (hpf) experienced decreased average heart rate after 2 days of PUA exposure. Embryos 24 hpf exposed to PUA mixtures for 6 days showed a reduction in size in comparison to embryos from controls. Embryos exposed to PUAs from 2 hpf until death showed lower survivorship compared to larvae in controls. The results of this study suggest that larval fish that are sympatric with PUA producing diatoms during their embryonic and larval stages may be susceptible to detrimental effects from PUA exposure.</p>","PeriodicalId":11497,"journal":{"name":"Ecotoxicology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142754757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
EcotoxicologyPub Date : 2024-11-28DOI: 10.1007/s10646-024-02832-1
Debkanta Ghosh, Samir Kumar Saha, Anilava Kaviraj, Subrata Saha
{"title":"Activity pattern of antioxidant enzymes in relation to the time of exposure of hexavalent chromium to Nile tilapia Oreochromis niloticus.","authors":"Debkanta Ghosh, Samir Kumar Saha, Anilava Kaviraj, Subrata Saha","doi":"10.1007/s10646-024-02832-1","DOIUrl":"https://doi.org/10.1007/s10646-024-02832-1","url":null,"abstract":"<p><p>Hexavalent chromium (Cr (VI)), a toxicant of environmental concern, frequently enters into water bodies and produces oxidative stress in fish. The antioxidant enzymes, Superoxide dismutase (SOD), Catalase (CAT), and Glutathion S-transferase (GST) are activated to counteract the oxidative stress in fish. This study explores the pattern of activation of these enzymes in gill, muscle, liver, and kidney tissues of Nile tilapia Oreochromis niloticus exposed to 9.35 mg/L and 18.70 mg/L of Cr (VI) for 96 h. The optimal hour of activity of these enzymes was revealed through extensive regression analysis. The results indicate a bell-shaped time response curve in the activity of the enzymes in both the treatments, except CAT in the gill of fish exposed to 18.70 mg/L Cr (VI) and GST in the gill, liver, and kidney of fish exposed to 18.70 mg/L Cr (VI). The results indicate that the optimal hour of activity of SOD changes in tandem with CAT, SOD responding first followed by CAT, both diminishing within 96 h. However, deviating from the bell-shaped pattern, the activity of CAT in gill and GST in gill, liver, and kidney in fish exposed to 18.70 mg/L Cr (VI) continued to rise even at 96 h, indicating that these antioxidant enzymes could not diminish the oxidative stress produced by the higher dose of Cr (VI). It was concluded that the activity of SOD, CAT, and GST between 30 and 70 h in the gill, liver, and kidney of Nile tilapia could serve as excellent biomarkers of oxidative stress under low doses of Cr (VI).</p>","PeriodicalId":11497,"journal":{"name":"Ecotoxicology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142738708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
EcotoxicologyPub Date : 2024-11-23DOI: 10.1007/s10646-024-02829-w
Bruna Ferrari Schedenffeldt, Bruno Barburgian Ramalho Siqueira, Rafaela Oliva da Silva, Patrícia Andrea Monquero
{"title":"Evaluation of herbicide toxicity to Lemna minor L. and Azolla caroliniana Willd.","authors":"Bruna Ferrari Schedenffeldt, Bruno Barburgian Ramalho Siqueira, Rafaela Oliva da Silva, Patrícia Andrea Monquero","doi":"10.1007/s10646-024-02829-w","DOIUrl":"10.1007/s10646-024-02829-w","url":null,"abstract":"<p><p>Long-residual herbicides can negatively impact aquatic ecosystems through surface runoff and leaching. This study evaluated the acute toxicity of indaziflam, sulfentrazone, clomazone, and tebuthiuron on Lemna minor and Azolla caroliniana. Experiments were conducted in the laboratory using a completely randomized design (CRD) with six concentrations of each herbicide across four replicates. The study assessed the concentration causing acute effects in 50% of the organisms (EC<sub>50</sub>), the effects on fresh weight (g), and the risk quotient (RQ). For L. minor, EC<sub>50</sub> values were 0.41, 0.73, 0.12, and 0.21 mg L⁻¹ for clomazone, sulfentrazone, indaziflam, and tebuthiuron, respectively; and for A. caroliniana, the values were 3.26, 0.51, 0.11, and 0.71 mg L⁻¹, respectively. All tested herbicides were classified as highly toxic to macrophyte species, except for clomazone, which was categorized as 'moderately toxic' for A. caroliniana. Moreover, these herbicides significantly reduced fresh weight even at the lowest concentrations.</p>","PeriodicalId":11497,"journal":{"name":"Ecotoxicology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142695279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
EcotoxicologyPub Date : 2024-11-20DOI: 10.1007/s10646-024-02833-0
Michał Urbański, Burak Mete Yiğit, Anna Ekner-Grzyb, Jagna Chmielowska-Bąk
{"title":"Physiological and oxidative status of soybean seedlings exposed to short term treatment with polystyrene nanoparticles.","authors":"Michał Urbański, Burak Mete Yiğit, Anna Ekner-Grzyb, Jagna Chmielowska-Bąk","doi":"10.1007/s10646-024-02833-0","DOIUrl":"https://doi.org/10.1007/s10646-024-02833-0","url":null,"abstract":"<p><p>Plastic is widely used worldwide due to its durability and relatively low production costs. However, its durability also has significant drawbacks - plastic is a slowly degrading material and greatly contributes to the environmental pollution. Increasing body of evidence shows that contamination of the environment with plastic negatively affects plants and other living organisms. The aim of present research was to determine whether short-term exposure to polystyrene nanoparticles (PSNP) has toxic effect on soybean seedlings (Glycine max L). In the first stage of the research, the effect of two hour long incubation in PSNP solutions (10 and 100 mgl<sup>-1</sup>) on the germination of soybean seeds was determined. In the second part of the study, the potential cytotoxic effect of PSNP on young seedlings was measured. The results indicate that incubation in PSNP solutions inhibits the germination of soybean seeds by approx. 10% (at p = 0.05). However, this effect was only observed after 48 and 72 h of germination and by lower PSNP concentrations, 10 mgl<sup>-1</sup>. In turn, in young soybean seedlings exposure to PSNP had no effect on growth, cell viability or oxidative status by p = 0.05. The results indicate that germination is a PSNP-sensitive process. In turn, already germinated seedlings are relatively resistant to the short-term exposure to this stressor.</p>","PeriodicalId":11497,"journal":{"name":"Ecotoxicology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142675456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
EcotoxicologyPub Date : 2024-11-20DOI: 10.1007/s10646-024-02830-3
Andrés Egea-Serrano, Elaine Macêdo, Mirco Solé
{"title":"Sensitivity of larval Dendropsophus haddadi (Bastos and Pombal 1996), an anuran species from the Brazilian Atlantic Forest, to acute exposure to nitrate.","authors":"Andrés Egea-Serrano, Elaine Macêdo, Mirco Solé","doi":"10.1007/s10646-024-02830-3","DOIUrl":"https://doi.org/10.1007/s10646-024-02830-3","url":null,"abstract":"<p><p>Nitrogenous pollution has been reported to be a major threat to biodiversity and, therefore, it may be related to the decline of amphibians, the most threatened group of vertebrates in the world. In spite of this, and the widespread release of nitrogenous compounds into the environment, research on the impact of this pollution on Neotropical species remains limited. The aim of this study was to assess the sensitivity of one anuran species inhabiting in the Brazilian Atlantic Forest (Dendropsophus haddadi) to NaNO<sub>3</sub> by an acute exposure laboratory experiment, addressing also whether carryover effects exist. Larval mortality increased as time went on in the case of the highly polluted treatments, which also affected swimming performance. Mean lethal concentration (LC<sub>50</sub>) values revealed that the sensitivity of the study species to NaNO<sub>3</sub> was similar to that described for other amphibians, both tropical and temperate species. Additionally, larvae exposed to moderate levels of pollution significantly increased final mass. In spite of this, once larvae were transfered into clean water, no further mortality was recorded, and swimming performance improved. These results indicate that tropical species may not be more vulnerable to chemical pollution than temperate ones, and suggest that they are able to recover from acute short exposure to nitrogenous compounds, all of which represent a new addition to the knowledge of the vulnerability of Dendropsophus haddadi to pollution and, thus, to Neotropical ecotoxicology.</p>","PeriodicalId":11497,"journal":{"name":"Ecotoxicology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142675457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
EcotoxicologyPub Date : 2024-11-20DOI: 10.1007/s10646-024-02835-y
Álvaro Alonso, M C Llandres-Díez, P Cruces-Estepa
{"title":"Contrasting behavioural responses to concurrent stressors in an aquatic snail: the importance of stress type and combination.","authors":"Álvaro Alonso, M C Llandres-Díez, P Cruces-Estepa","doi":"10.1007/s10646-024-02835-y","DOIUrl":"https://doi.org/10.1007/s10646-024-02835-y","url":null,"abstract":"<p><p>Behaviour is a fundamental parameter for understanding the animal fitness, serving as an indicator of exposure to stressors. In ecosystems, animals often face multiple stressors simultaneously. Their behavioural responses may vary when exposed to individual stressors, whilst synergistic, additive, or antagonistic effects can result from the interaction of multiple stressors. Therefore, it is imperative to conduct studies that take into account the common occurrence of multi-stress scenarios in aquatic ecosystems. We tested the effects of three sources of stress (acidity (A), toxicity with acetone (T) and conspecific chemical cues (S)) on the behaviour of the aquatic snail Potamopyrgus antipodarum (Tateidae, Mollusca). We evaluated the impact of each stressor, as well as in combinations of two or three stressors simultaneously. The highest time to initiate movement was shown by the animals of the low water pH (A) followed by those exposed to the combination of low water pH and acetone exposure (AT). The differences between the time to initiate movement of each treatment with control revealed a marked decrease in the differences for the snails from the conspecific chemical cues (S) and ST treatments, which mean a higher time to initiate movements. It is concluded that behaviour varied depending on the source and combination of stress. While an acid environment and conspecific signals had contrasting effects when applied separately, their simultaneous exposure resulted in no significant impact. This highlights the importance of considering the combined effects of multiple stressors when extrapolating laboratory results to real-world scenarios, where organisms are often exposed to more than one stressor at a time.</p>","PeriodicalId":11497,"journal":{"name":"Ecotoxicology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142675454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}