Development最新文献_第4页

Sox8 and Sox9 regulate differentiation and nuclear positioning of retinal Müller glia. Sox8和Sox9调控视网膜<s:1>神经胶质细胞的分化和核定位。

IF 4 2区生物学

Development Pub Date : 2026-10-01 Epub Date: 2026-10-13 DOI: 10.1242/dev.205508

Nicole Pannullo, Debarpita Datta, Clayton P Santiago, Jared A Tangeman, Lizhi Jiang, Leighton H Duncan, Taqdees Gohar, Megan Gimmen, Seth Blackshaw

{"title":"Sox8 and Sox9 regulate differentiation and nuclear positioning of retinal Müller glia.","authors":"Nicole Pannullo, Debarpita Datta, Clayton P Santiago, Jared A Tangeman, Lizhi Jiang, Leighton H Duncan, Taqdees Gohar, Megan Gimmen, Seth Blackshaw","doi":"10.1242/dev.205508","DOIUrl":"https://doi.org/10.1242/dev.205508","url":null,"abstract":"Temporal patterning of retinal progenitor cells governs the sequential generation of retinal cell types, with gliogenesis occurring late in development. Sox8 and Sox9, members of the SoxE transcription factor family, are highly expressed in late-stage retinal progenitor cells and mature Müller glia, yet their functional roles remain incompletely defined. Here, we have employed gain- and loss-of-function approaches, single-cell multiomic profiling, and injury models to investigate Sox8 and Sox9 function. Overexpression of human SOX8 and SOX9 in early-stage mouse retinal progenitor cells both suppressed generation of early-born cells, consistent with a role in late-stage temporal identity, and induced ectopic generation of oligodendrocyte lineage-like cells. Conversely, conditional deletion of Sox8 and/or Sox9 in late-stage progenitors did not impair Müller glia specification, but caused radial displacement of glial nuclei into the outer retina and reduced expression of multiple markers of mature Müller glia. Loss of Sox8 and/or Sox9 in mature Müller glia modestly increased proliferation post-injury without inducing neurogenic competence. These findings suggest that Sox8 and Sox9 are dispensable for gliogenesis and repression of neurogenic competence, but are essential for proper laminar positioning and full terminal differentiation of retinal Müller glia.","PeriodicalId":11375,"journal":{"name":"Development","volume":"153 19","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149180065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

C. elegans dauer morphogenesis requires inhibition of DBL-1/BMP signaling by ztf-30. 秀丽隐杆线虫的形态发生需要ztf-30抑制DBL-1/BMP信号。

IF 4 2区生物学

Development Pub Date : 2026-10-01 Epub Date: 2026-09-30 DOI: 10.1242/dev.205808

Luciana F Godoy, M Eugenia Páez, Nicolás R Salvatore, Yvan Doctorovich, Judith L Yanowitz, Daniel Hochbaum

{"title":"C. elegans dauer morphogenesis requires inhibition of DBL-1/BMP signaling by ztf-30.","authors":"Luciana F Godoy, M Eugenia Páez, Nicolás R Salvatore, Yvan Doctorovich, Judith L Yanowitz, Daniel Hochbaum","doi":"10.1242/dev.205808","DOIUrl":"https://doi.org/10.1242/dev.205808","url":null,"abstract":"In response to changing environments, the nematode Caenorhabditis elegans enters a stress-resistant diapause stage to survive adverse conditions. While the pathways controlling developmental arrest, such as insulin/daf-2 and TGF-β/daf-7, are well characterized, mechanisms governing subsequent dauer morphogenesis remain poorly understood. Here, we report that the zinc-finger transcription factor ZTF-30 is dispensable for developmental arrest but essential for dauer morphogenesis. ztf-30 mutants form partial dauers that lack a protective thickened cuticle and specialized structures such as alae. Transcriptomics revealed that ztf-30 mutants precociously activate adult collagen expression and upregulate the DBL-1/BMP signaling pathway, which primarily modulates body size and innate immunity. These data suggest ZTF-30 represses BMP signaling. Consistent with this model, zt-f-30 partial dauers show increased dbl-1 expression, increased DBL-1 signaling phenocopies ztf-30 loss, and reducing DBL-1 signaling rescues the partial dauer phenotype. During larval growth, ztf-30 loss causes ectopic hypodermal dbl-1 expression and increased body length. Ultimately, our findings establish that the active inhibition of DBL-1/BMP signaling is strictly required for proper dauer morphogenesis and we identify ZTF-30 as an essential transcriptional repressor driving this developmental shutdown.","PeriodicalId":11375,"journal":{"name":"Development","volume":"153 19","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149079746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multi-gene biomarkers reveal spatial organization and subpopulation-specific damage response in biliary epithelial cells. 多基因生物标志物揭示胆道上皮细胞的空间组织和亚群特异性损伤反应。

IF 4 2区生物学

Development Pub Date : 2026-10-01 Epub Date: 2026-10-09 DOI: 10.1242/dev.205690

Kendall G Kanakanui, Fransky Hantelys, Hannah R Hrncir, Sergei Bombin, Adam D Gracz

{"title":"Multi-gene biomarkers reveal spatial organization and subpopulation-specific damage response in biliary epithelial cells.","authors":"Kendall G Kanakanui, Fransky Hantelys, Hannah R Hrncir, Sergei Bombin, Adam D Gracz","doi":"10.1242/dev.205690","DOIUrl":"10.1242/dev.205690","url":null,"abstract":"Biliary epithelial cell (BEC) heterogeneity remains challenging to define. Here, we performed scRNA-seq on Sox9EGFP+ liver epithelium from mice subjected to bile duct ligation (BDL) and sham controls. We identified 4 BEC subpopulations in sham controls, each associated with specific enrichment of a minimal one or two gene biomarker ('metagene'). Metagenes were validated by hybridization chain reaction fluorescence in situ hybridization, which also facilitated spatial analysis of intrahepatic bile ducts. BECs belonging to a given subpopulation were most likely to have neighbors of the same identity, forming homogenous cellular compartments within otherwise heterogeneous ducts. Subpopulation specific differential gene expression (DGE) analysis revealed that BDL decreases transcriptomic heterogeneity among BECs by suppressing subpopulation specific genes. BDL samples also included a proliferative subpopulation not found in controls, which contained populations enriched for three of the four metagenes. All BEC subpopulations were observed in vitro in monolayer cultures, where they clustered spatially with BECs of the same subtype. Together, our data demonstrate that metagene biomarkers facilitate spatial localization of BEC subpopulations in situ, identify subpopulation-specific injury responses and confirm that BEC heterogeneity is preserved in vitro.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149012045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Wnt2bb-triggered neighbor cell proliferation is essential for cloaca formation by inducing apoptosis in the cloaca through cell competition. wnt2bb触发的邻近细胞增殖是泄殖腔形成的必要条件，通过细胞竞争诱导泄殖腔凋亡。

IF 4 2区生物学

Development Pub Date : 2026-10-01 Epub Date: 2026-10-15 DOI: 10.1242/dev.205141

Yujie Wang, Mengna Yuan, Hao Wang, Zhengyu Liu, Liping Yang, Ying Yi Zhang, Hezhe Lu, Xinyu He, Qiang Wang

{"title":"Wnt2bb-triggered neighbor cell proliferation is essential for cloaca formation by inducing apoptosis in the cloaca through cell competition.","authors":"Yujie Wang, Mengna Yuan, Hao Wang, Zhengyu Liu, Liping Yang, Ying Yi Zhang, Hezhe Lu, Xinyu He, Qiang Wang","doi":"10.1242/dev.205141","DOIUrl":"10.1242/dev.205141","url":null,"abstract":"The cloaca serves as the only opening for the digestive, urinary and reproductive tracts in multiple species. Apoptosis is crucial for shaping the cloaca, yet the underlying mechanisms remain largely unclarified. Here, we find that Wnt2bb-triggered neighbor cell proliferation is essential for zebrafish cloaca formation by inducing apoptosis in the cloaca, likely through mechanical cell competition. wnt2bb is expressed robustly in the cloaca throughout its formation. wnt2bb inactivation downregulates nuclear accumulation of β-catenin and expression of the downstream target cyclin D1, severely suppressing peri-cloacal cell proliferation. The resulting decline in local cell density is associated with a biosensor-indicated reduction in mechanical compression on the cloacal cells, which prevents their competitive elimination and eventually causes cloacal obstruction. Moreover, palbociclib, a CDK4/6 inhibitor, not only inhibits peri-cloacal cell proliferation, but also abolishes apoptosis in the cloaca, generating similar defects in wild-type embryos. Overexpression of constitutively active β-catenin exclusively in the cells surrounding the cloaca, but not within the cloaca itself, is sufficient to rescue the cloacal obstruction. Thus, we uncover an important role of Wnt2bb-regulated cell competition during cloaca development.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148988665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanical anisotropy and medio-apical force transmission shape cellular strain heterogeneity in epithelia. 机械各向异性和中尖力传递形成上皮细胞应变异质性。

IF 4 2区生物学

Development Pub Date : 2026-09-24 DOI: 10.1242/dev.205417

Jing Yang, Yicheng Dong, Carter B Jones, Yingli Wang, Corey V Merino, Carsten Stuckenholz, Lance A Davidson

{"title":"Mechanical anisotropy and medio-apical force transmission shape cellular strain heterogeneity in epithelia.","authors":"Jing Yang, Yicheng Dong, Carter B Jones, Yingli Wang, Corey V Merino, Carsten Stuckenholz, Lance A Davidson","doi":"10.1242/dev.205417","DOIUrl":"10.1242/dev.205417","url":null,"abstract":"Mechanical forces play crucial roles in morphogenesis and function, yet individual cells often respond differently to the same external force. Strain heterogeneity, the non-uniform deformation of cells under uniform external mechanical stimuli, may underlie tissue-level robustness and guide morphogenetic outcomes. In this study, we applied a defined uniaxial strain to Xenopus laevis embryonic epithelial explants and investigated how strain was distributed at the single cell level. Our quantitative analysis revealed that cellular strain was heterogeneous, suggesting variable responses to a uniform mechanical stimulus. We found that cell intrinsic material properties, e.g., cell specific mechanical anisotropy had the strongest correlation with strain heterogeneity, suggesting a dominant role in variable mechanical response. We further analyzed how force was distributed at the cellular level using a vinculin force sensor and laser ablation. These experiments demonstrated that forces are primarily transmitted through the medio-apical actin cortex whereas junctional actin facilitates dissipation and remodeling. These findings provide new insights into the physical principles that underlie epithelial resilience and adaptive remodeling, highlighting the importance of distinct functions of junctional and medio-apical actin networks in mechanical adaptation.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149037054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pathway to Independence - an interview with Evan Healy. 独立之路-对Evan Healy的采访。

IF 4 2区生物学

Development Pub Date : 2026-09-15 Epub Date: 2026-09-17 DOI: 10.1242/dev.206072

引用次数: 0

Subepithelial myofibroblasts enhance stem cell regeneration after intestinal epithelial damage. 上皮下肌成纤维细胞促进肠上皮损伤后干细胞再生。

IF 4 2区生物学

Development Pub Date : 2026-09-15 Epub Date: 2026-09-28 DOI: 10.1242/dev.205629

Neil McCarthy, Camila Mendez Jimenez, Guodong Tie, Gizelle McCarthy, Shariq Madha-Krause, Ruiyang He, Ramesh A Shivdasani

{"title":"Subepithelial myofibroblasts enhance stem cell regeneration after intestinal epithelial damage.","authors":"Neil McCarthy, Camila Mendez Jimenez, Guodong Tie, Gizelle McCarthy, Shariq Madha-Krause, Ruiyang He, Ramesh A Shivdasani","doi":"10.1242/dev.205629","DOIUrl":"10.1242/dev.205629","url":null,"abstract":"When intestinal stem cells (ISCs) are damaged, other epithelial cells de-differentiate to restore the ISC compartment. Immune, nerve and lymphatic endothelial cells contribute signals that promote this response, but it is unclear whether or how the native mesenchymal niche senses and responds to ISC loss. In homeostasis, trophocytes and smooth muscle beneath intestinal crypts secrete BMP inhibitors (BMPi) and R-spondins to foster ISC self-renewal, whereas sub-epithelial myofibroblasts (SEMFs) concentrated at crypt tops promote differentiation through bone morphogenetic proteins (BMPs). We assessed transcriptional responses in mouse sub-epithelial populations after ablating ISCs with whole-body irradiation and treating Lgr5DTR mice with diphtheria toxin. More than any other cell type, SEMFs modulated expression of genes related to ISC expansion. However, the BMPi gene Smoc1 and non-canonical Wnt signals, both expressed in SEMFs after whole-body irradiation, were dispensable for ISC restoration. Instead, SEMFs appear to support ISC regeneration through factors that are not induced by ISC attrition per se but by local inflammation. These findings reveal unexpectedly limited transcriptional modulation of the native niche after ISC losses and context-dependent signaling functions for SEMFs.","PeriodicalId":11375,"journal":{"name":"Development","volume":"153 18","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13677008/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149069409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A protoplast-based method to visualize early cell biological events in plant cellular reprogramming and regeneration. 基于原生质体的植物细胞重编程和再生早期细胞生物学事件可视化方法。

IF 4 2区生物学

Development Pub Date : 2026-09-15 DOI: 10.1242/dev.205715

Kelsey M Reed, Abigail A Masri, Andrew J Hanrahan, Emery L Ng, Mao Li, Bastiaan O R Bargmann

{"title":"A protoplast-based method to visualize early cell biological events in plant cellular reprogramming and regeneration.","authors":"Kelsey M Reed, Abigail A Masri, Andrew J Hanrahan, Emery L Ng, Mao Li, Bastiaan O R Bargmann","doi":"10.1242/dev.205715","DOIUrl":"10.1242/dev.205715","url":null,"abstract":"Plant developmental biology lacks cell-based experimental systems comparable to the organoids and live-imaging platforms that have transformed mechanistic discovery in animal research. To address this gap, we present a robust, trackable protoplast regeneration platform in Arabidopsis thaliana that enables high-resolution, time-resolved analysis from the single-cell stage through microcolony formation and early regenerative development. Protoplasts are embedded in thin alginate matrices containing fluorescent fiducial beads, maintaining physical separation and allowing repeated return to the same cells over days to weeks. This design supports long-term imaging using epifluorescence, confocal and lattice light-sheet microscopy, enabling visualization of cell-cycle re-entry, asymmetric division, organelle dynamics, dedifferentiation, redifferentiation and regenerative competence. Fluorescent reporters for nuclei, membranes, microtubules, Golgi and hormone signaling further permit observation of subcellular organization and signaling heterogeneity during early reprogramming. Together, this platform provides an accessible, scalable system for studying plant cellular plasticity at single-cell resolution and offers a foundation for plant organoid-like models. By enabling high-resolution study of regeneration from single cells, this method expands the experimental toolkit and supports efforts to overcome species- and genotype-dependent barriers to transformation and plant biotechnology.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148910841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In preprints - when drift is not neutral: evidence from lifelong monitoring of zebrafish spermatogenesis. 在预印本中——当漂移不是中性的时候：来自斑马鱼精子发生的终身监测的证据。

IF 4 2区生物学

Development Pub Date : 2026-09-15 Epub Date: 2026-09-17 DOI: 10.1242/dev.206040

Muhammad Shahan Anwar, Yue Rong Tan, Chen-Hui Chen

引用次数: 0

Histone H3K9 methyltransferases regulate cortical growth by coordinating heterochromatin formation and neural progenitor dynamics. 组蛋白H3K9甲基转移酶通过协调异染色质形成和神经祖细胞动力学来调节皮质生长。

IF 4 2区生物学

Development Pub Date : 2026-09-15 Epub Date: 2026-09-21 DOI: 10.1242/dev.205584

Sophie Warren, Chris Hemmerich, Ram Podicheti, José-Manuel Baizabal

{"title":"Histone H3K9 methyltransferases regulate cortical growth by coordinating heterochromatin formation and neural progenitor dynamics.","authors":"Sophie Warren, Chris Hemmerich, Ram Podicheti, José-Manuel Baizabal","doi":"10.1242/dev.205584","DOIUrl":"10.1242/dev.205584","url":null,"abstract":"Packaging of DNA into heterochromatin is a fundamental mechanism of transcriptional silencing, yet its role in regulating neural progenitor behavior during brain development remains poorly understood. Trimethylation of histone H3 lysine 9 (H3K9me3), catalyzed by the methyltransferases SETDB1, SUV39H1 and SUV39H2, is a defining feature of heterochromatin, but functional redundancy among these enzymes has obscured their developmental roles. Here, we generated a cortex-specific triple knockout mouse model lacking Setdb1, Suv39h1 and Suv39h2 to directly interrogate H3K9me3 function during corticogenesis. Combined loss of H3K9 methyltransferases caused genome-wide depletion of H3K9me3, disruption of neural progenitor cell-cycle progression, and impaired cortical neurogenesis, resulting in microcephaly. H3K9 methyltransferases preserve neural progenitor identity and function by silencing clustered protocadherins, meiosis-associated genes and a cell-cycle restraint program through H3K9me3 deposition. Loss of H3K9me3 promoted local chromatin opening and increased transcription factor occupancy, enabling transposable elements to acquire cryptic enhancer activity and modulate proximal gene expression. Together, these findings establish H3K9me3 heterochromatin as an active regulator of neural progenitor dynamics and lineage fidelity, revealing a central epigenetic mechanism that restricts aberrant transcriptional programs to ensure cortical growth.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148905363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0