Development最新文献_第4页

The WIRS motifs in Fat2 are required for Drosophila egg chamber rotation but not for elongation. Fat2中的WIRS基序是果蝇卵室旋转所必需的，但不是延长所必需的。

IF 3.7 2区生物学

Development Pub Date : 2025-01-15 Epub Date: 2025-01-17 DOI: 10.1242/dev.204201

Akanksha Bhatt, Valentin Ruffine, Uwe Töpfer, Jinhee Ryu, Elisabeth Fischer-Friedrich, Christian Dahmann

{"title":"The WIRS motifs in Fat2 are required for Drosophila egg chamber rotation but not for elongation.","authors":"Akanksha Bhatt, Valentin Ruffine, Uwe Töpfer, Jinhee Ryu, Elisabeth Fischer-Friedrich, Christian Dahmann","doi":"10.1242/dev.204201","DOIUrl":"10.1242/dev.204201","url":null,"abstract":"The elongation of tissues and organs is important for proper morphogenesis in animal development. In Drosophila ovaries, the elongation of egg chambers involves aligned Collagen IV fiber-like structures, a gradient of extracellular matrix stiffness and actin-based protrusion-driven collective cell migration, leading to the rotation of the egg chamber. Egg chamber elongation and rotation depend on the atypical cadherin Fat2. Fat2 contains in its intracellular region three WRC interacting receptor sequence (WIRS) motifs, which previously had been shown to bind to the WAVE regulatory complex (WRC), a conserved actin regulator. Here, we show that in fat2 mutant flies lacking the WIRS motifs, egg chambers fail to rotate and Collagen IV fiber-like structures are impaired, yet a gradient of extracellular matrix stiffness is established and egg chambers properly elongate. We conclude that the WIRS motifs are required for egg chamber rotation and that egg chamber rotation might be a prerequisite for proper formation of Collagen IV fiber-like structures. Egg chamber rotation, however, is dispensable for extracellular matrix stiffness gradient formation and for egg chamber elongation.","PeriodicalId":11375,"journal":{"name":"Development","volume":"152 2","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11829772/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143001989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In preprints: giving the developing brain the energy it needs. 在预印本中：给予发育中的大脑所需的能量。

IF 3.7 2区生物学

Development Pub Date : 2025-01-15 Epub Date: 2025-01-16 DOI: 10.1242/dev.204594

Taylor R Pennington, Madeline G Andrews

引用次数: 0

Transcriptional dynamics during karyogamy in rice zygotes. 水稻受精卵核分裂过程中的转录动力学。

IF 3.7 2区生物学

Development Pub Date : 2025-01-15 Epub Date: 2025-01-27 DOI: 10.1242/dev.204497

Erika Toda, Shizuka Koshimizu, Atsuko Kinoshita, Tetsuya Higashiyama, Takeshi Izawa, Kentaro Yano, Takashi Okamoto

{"title":"Transcriptional dynamics during karyogamy in rice zygotes.","authors":"Erika Toda, Shizuka Koshimizu, Atsuko Kinoshita, Tetsuya Higashiyama, Takeshi Izawa, Kentaro Yano, Takashi Okamoto","doi":"10.1242/dev.204497","DOIUrl":"10.1242/dev.204497","url":null,"abstract":"Upon fertilization, male and female nuclei fuse to form the zygotic nucleus in angiosperms. Karyogamy is considered to be essential for proper embryogenesis; however, the transcriptional dynamics during karyogamy in plant zygotes remain unclear. In this study, we performed a single-cell transcriptome analysis of rice zygotes at six early developmental stages (15 min, 30 min, 1 h, 2 h, 4 h, and 6 h after gamete fusion) to reveal gene expression profiles during karyogamy in plant zygotes. The time-series RNA-sequencing analysis detected possible de novo and altered gene expression in zygotes from 15 min post-fertilization. Fertilization-induced transcription during karyogamy was characterized by protein interaction database and gene ontology (GO) analyses. Furthermore, paternal allele transcription was initiated approximately 30 min to 1 h after gamete fusion, when nuclear fusion begins in the zygote. Some transcripts preferentially expressed in egg cells were downregulated after gamete fusion. Moreover, a dynamic shift from maternal-biased transcripts to bi-parental expression occurred during early zygotic development. These results suggest that transcriptional dynamics during karyogamy plays an initial role in proper and sequential zygotic development and embryogenesis.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11829756/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142946436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A case for broadening our view of mechanism in developmental biology. 一个扩展我们对发育生物学机制观点的案例。

IF 3.7 2区生物学

Development Pub Date : 2025-01-15 Epub Date: 2025-01-16 DOI: 10.1242/dev.204605

B Duygu Özpolat, Swathi Arur, Mansi Srivastava

引用次数: 0

Positive autoregulation of Sox17 is necessary for gallbladder and extrahepatic bile duct formation. Sox17的阳性自动调节对于胆囊和肝外胆管的形成是必要的。

IF 3.7 2区生物学

Development Pub Date : 2025-01-15 Epub Date: 2025-01-16 DOI: 10.1242/dev.203033

Linh T Trinh, Ryan R Finnel, Anna B Osipovich, Jessica R Musselman, Leesa L Sampson, Christopher V E Wright, Mark A Magnuson

引用次数: 0

Optimized husbandry and targeted gene-editing for the cnidarian Nematostella vectensis. 刺胞线虫病的优化饲养和靶向基因编辑。

IF 3.7 2区生物学

Development Pub Date : 2025-01-15 Epub Date: 2025-02-04 DOI: 10.1242/dev.204387

João E Carvalho, Maxence Burtin, Olivier Detournay, Aldine R Amiel, Eric Röttinger

{"title":"Optimized husbandry and targeted gene-editing for the cnidarian Nematostella vectensis.","authors":"João E Carvalho, Maxence Burtin, Olivier Detournay, Aldine R Amiel, Eric Röttinger","doi":"10.1242/dev.204387","DOIUrl":"10.1242/dev.204387","url":null,"abstract":"Optimized laboratory conditions for research models are crucial for the success of scientific projects. This includes controlling the entire life cycle, having access to all developmental stages and maintaining stable physiological conditions. Reducing the life cycle of a research model can also enhance the access to biological material and speed up genetic tool development. Thus, we optimized the rearing conditions for the sea anemone Nematostella vectensis, a cnidarian research model, to study embryonic and post-metamorphic processes, such as regeneration. We adopted a semi-automated aquaculture system for N. vectensis and developed a dietary protocol optimized for the different life stages. Thereby, we increased spawning efficiencies, juvenile growth and survival rates, and considerably reduced the overall life cycle down to 2 months. To further improve the obtention of CRISPR-Cas9 mutants, we optimized the design of sgRNAs leading to full knockout animals in F0 polyps using a single sgRNA. Finally, we show that NHEJ-mediated transgene insertion is possible in N. vectensis. In summary, our study provides additional resources for the scientific community that uses or plans to use N. vectensis as a research model.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142946429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An interview with Sarah Bray.

IF 3.7 2区生物学

Development Pub Date : 2025-01-15 Epub Date: 2025-02-03 DOI: 10.1242/dev.204645

引用次数: 0

Abnormal H3K27me3 underlies degenerative spermatogonial stem cells in cryptorchid testis. H3K27me3异常是隐睾退行性精原干细胞的基础。

IF 3.7 2区生物学

Development Pub Date : 2025-01-15 Epub Date: 2025-01-16 DOI: 10.1242/dev.204239

Kazushige Kuroha, Ivana Dočkal, Uroš Radović, Kuniko Nakajima, Ikue Hoshi, Shion Matsuda, Noriko Kojitani, Kazuyuki Ohbo, Shin-Ichi Tomizawa

{"title":"Abnormal H3K27me3 underlies degenerative spermatogonial stem cells in cryptorchid testis.","authors":"Kazushige Kuroha, Ivana Dočkal, Uroš Radović, Kuniko Nakajima, Ikue Hoshi, Shion Matsuda, Noriko Kojitani, Kazuyuki Ohbo, Shin-Ichi Tomizawa","doi":"10.1242/dev.204239","DOIUrl":"10.1242/dev.204239","url":null,"abstract":"Cryptorchidism is the most frequent congenital defect in newborn males characterized by the absence of the testis from the scrotum. Approximately 90% of individuals with untreated bilateral cryptorchidism exhibit azoospermia due to defective spermatogenesis in the affected testis. Although abnormal spermatogonial stem cell maintenance or differentiation is suggested to cause germ cell degeneration in the cryptorchid testis, the underlying molecular mechanisms remain unclear. Here, we profiled spermatogonial epigenetic landscapes using surgically induced cryptorchid testis in the mouse. We show that cryptorchidism leads to alterations in local, but not global, H3K27me3 and H3K9me3 in undifferentiated spermatogonia. Of these, the loss of H3K27me3 was correlated with activation of developmental and proapoptotic pathway genes that are repressed by the polycomb machinery in germ cells. Cryptorchid spermatogonia exhibit an increase of the H3K27me3 demethylases KDM6A and KMD6B. Furthermore, we reveal that an increased temperature leads to Kdm6a/b upregulation in germline stem cells cultured in vitro. Thus, our study suggests that temperature-dependent histone demethylation may induce mRNA dysregulation due to the partial loss of H3K27me3 in spermatogonia.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11829757/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142913931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nup107 contributes to the maternal-to-zygotic transition by preventing the premature nuclear export of pri-miR427. Nup107通过阻止pri-miRNA 427过早的核输出来促进母代向合子的转变。

IF 3.7 2区生物学

Development Pub Date : 2025-01-15 Epub Date: 2025-02-04 DOI: 10.1242/dev.202865

Valentyna Kostiuk, Rakib Kabir, Kaitlin Levangie, Stefany Empke, Kimberly Morgan, Nick D L Owens, C Patrick Lusk, Mustafa K Khokha

{"title":"Nup107 contributes to the maternal-to-zygotic transition by preventing the premature nuclear export of pri-miR427.","authors":"Valentyna Kostiuk, Rakib Kabir, Kaitlin Levangie, Stefany Empke, Kimberly Morgan, Nick D L Owens, C Patrick Lusk, Mustafa K Khokha","doi":"10.1242/dev.202865","DOIUrl":"10.1242/dev.202865","url":null,"abstract":"Emerging evidence suggests that the nuclear pore complex can have unique compositions and distinct nucleoporin functions in different cells. Here, we show that Nup107, a key component of the NPC scaffold, varies in expression over development: it is expressed at higher levels in the blastula compared to the gastrula, suggesting a crucial role before gastrulation in Xenopus. We find that depletion of Nup107 affects the differentiation of the early germ layers leading to an expansion of the ectoderm at the expense of endoderm and mesoderm. By analyzing an RNA-sequencing time course, we observed that depletion of Nup107 affects the maternal-zygotic transition by delaying the degradation of maternal transcripts that occurs as zygotic transcription begins. The transcripts are enriched in recognition sites for miR427, a conserved microRNA that destabilizes maternal transcripts including REST, which encodes a Kruppel-type zinc-finger transcription factor that we demonstrate is crucial for ectodermal cell fates. Mechanistically, we show that Nup107 is required to prevent the premature export of pri-miR427 transcript before processing. Nup107 depletion leads to the reduced production of mature miR427 and maternal transcript stabilization. We conclude that high levels of Nup107 in the early embryo are crucial for the nuclear retention and subsequent processing of pri-miR427 transcripts that is required for timely maternal RNA clearance to enable gastrulation.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11829755/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142946428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Canonical Wnt signalling from the area opaca induces and maintains the marginal zone in pre-primitive-streak stage chick embryos. 来自不透明区的典型Wnt信号在原始条纹期前的鸡胚胎中诱导和维持边缘区。

IF 3.7 2区生物学

Development Pub Date : 2025-01-15 Epub Date: 2025-01-23 DOI: 10.1242/dev.204350

Yara Fadaili, Hui-Chun Lu, Hyung Chul Lee, Amra Ryazapova, Claudio D Stern

{"title":"Canonical Wnt signalling from the area opaca induces and maintains the marginal zone in pre-primitive-streak stage chick embryos.","authors":"Yara Fadaili, Hui-Chun Lu, Hyung Chul Lee, Amra Ryazapova, Claudio D Stern","doi":"10.1242/dev.204350","DOIUrl":"10.1242/dev.204350","url":null,"abstract":"In chick embryos before primitive streak formation, the outermost extra-embryonic region, known as the area opaca (AO), was generally thought to act only by providing nutrients and mechanical support to the embryo. Immediately internal to the AO is a ring of epiblast called the marginal zone (MZ), separating the former from the inner area pellucida (AP) epiblast. The MZ does not contribute cells to any part of the embryo but is involved in determining the position of primitive streak formation from the adjacent AP epiblast. Recently, it was discovered that the AO can induce an MZ from AP epiblast. Here, we explore the nature of this inductive signal. We find that WNT8C is highly expressed in the AO, whereas canonical Wnt pathway targets are enriched in the MZ, along with strong nuclear β-catenin localization. Using isolation and recombination experiments combined with gain- and loss-of-function by exogenous chemical modulators of the pathway, we reveal that Wnt signalling is essential for induction and maintenance of the MZ, as well as sufficient to induce MZ properties in AP epiblast. We propose that canonical Wnt signalling is responsible for induction of the MZ by the area opaca.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11829775/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142970058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0