IF 3.6 2区生物学

Development Pub Date : 2025-10-15 Epub Date: 2025-08-07 DOI: 10.1242/dev.205106

N Sumru Bayin, Benjamin Steventon, Mekayla Storer

引用次数: 0

Mesenchymal SLMAP coordinates with MST3 to govern gut elongation during development. 间充质SLMAP与MST3协同控制发育过程中的肠伸长。

IF 3.7 2区生物学

Development Pub Date : 2025-10-15 Epub Date: 2025-07-09 DOI: 10.1242/dev.204483

Yuwei Pan, Shiyang Wang, Wuqi Yang, Xi Wu, Hanfu Zhang, Sujuan Du, Mingxin Zhang, Liyuan Hou, Maksim V Plikus, Jianwei Shuai, Cong Lv, Lu Yu, Zhengquan Yu

{"title":"Mesenchymal SLMAP coordinates with MST3 to govern gut elongation during development.","authors":"Yuwei Pan, Shiyang Wang, Wuqi Yang, Xi Wu, Hanfu Zhang, Sujuan Du, Mingxin Zhang, Liyuan Hou, Maksim V Plikus, Jianwei Shuai, Cong Lv, Lu Yu, Zhengquan Yu","doi":"10.1242/dev.204483","DOIUrl":"10.1242/dev.204483","url":null,"abstract":"Developing gut in mice undergoes rapid elongation during late embryogenesis, yet significantly slows down after birth. The precise regulatory mechanism of this dynamic morphogenetic process remains unknown. By utilizing single-cell RNA-sequencing analysis, we show that YAP activity in intestinal fibroblasts is the major molecular contributor to gut elongation. To determine how mesenchymal YAP activity is controlled, we identified canonical sarcolemma membrane-associated protein (SLMAP) as its critical regulator during mouse embryonic gut morphogenesis. Deleting Slmap in gut mesenchyme impairs YAP activity, leading to a short gut and a significant decrease in intestinal epithelial cell proliferation. Mechanistically, SLMAP activates YAP by directly regulating MST3 kinase. Physiologically, MST3 levels prominently increase over the developmental time, reaching their peak on postnatal day (P)14, when gut elongation in mice slows down. Depleting Mst3 in mesenchyme results in increased gut length at P14 accompanied by enhanced YAP activity. Importantly, a short gut phenotype in mesenchyme-specific Slmap mutant mice is partially compensated for by concomitant deletion of mesenchymal Mst3. Taken together, our findings demonstrate that SLMAP interacts with MST3 kinase to regulate the mesenchymal YAP activity that governs dynamic gut elongation across embryonic and postnatal development.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144301363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nelfb promotes dermal white adipose tissue formation through RNA polymerase II-mediated adipogenic gene regulation. Nelfb通过RNA聚合酶II介导的脂肪生成基因调控促进皮肤白色脂肪组织的形成。

IF 3.6 2区生物学

Development Pub Date : 2025-10-15 Epub Date: 2025-10-10 DOI: 10.1242/dev.204976

Samiksha Mahapatra, Julian Gomez, Uyanga Batzorig, Ye Liu, Celia Fernández-Méndez, Yifang Chen, George L Sen

{"title":"Nelfb promotes dermal white adipose tissue formation through RNA polymerase II-mediated adipogenic gene regulation.","authors":"Samiksha Mahapatra, Julian Gomez, Uyanga Batzorig, Ye Liu, Celia Fernández-Méndez, Yifang Chen, George L Sen","doi":"10.1242/dev.204976","DOIUrl":"10.1242/dev.204976","url":null,"abstract":"Dermal white adipose tissue (dWAT) is crucial for skin homeostasis, contributing to hair follicle regeneration, immune defense and skin wound healing. dWAT is formed and maintained by the differentiation of adipocyte precursors found in the dermis of the skin. While transcription factors that control adipocyte differentiation have been well characterized, other aspects of transcription control, such as pausing/elongation, are poorly understood. Here, we show that deletion of the transcriptional pause factor, Nelfb, from preadipocyte lineages in mice led to a failure of dWAT and other fat depot formation, perinatal lethality and reduced expression of adipogenic genes. Nelfb promotes an open chromatin structure and stabilizes RNA Polymerase II binding to Pparg, Cebpa, Krox20 and Stat3 to allow their transcription, which is necessary for adipocyte differentiation. Retroviral expression of Pparg in Nelfb-depleted cells restored adipocyte differentiation in cultured cells, while treatment of Nelfb-deleted mice with the Pparg agonist, rosiglitazone, allowed for dWAT formation and prolonged lifespan. These findings highlight the essential role of Nelfb in promoting the expression of key adipogenic genes that are necessary for dWAT formation and adipocyte differentiation.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145074609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An interview with Mansi Srivastava. 对Mansi Srivastava的采访。

IF 3.7 2区生物学

Development Pub Date : 2025-10-15 Epub Date: 2025-06-17 DOI: 10.1242/dev.204952

引用次数: 0

A developmental atlas of zebrafish gills links early vascular patterning to adult architecture. 斑马鱼鳃的发育图谱将早期血管模式与成年结构联系起来。

IF 3.6 2区生物学

Development Pub Date : 2025-10-15 Epub Date: 2025-09-15 DOI: 10.1242/dev.204984

Mathieu Preußner, Anna Mertens, Marion Basoglu, Virginie Lecaudey

{"title":"A developmental atlas of zebrafish gills links early vascular patterning to adult architecture.","authors":"Mathieu Preußner, Anna Mertens, Marion Basoglu, Virginie Lecaudey","doi":"10.1242/dev.204984","DOIUrl":"10.1242/dev.204984","url":null,"abstract":"Gills are essential for fish respiration and have a highly specialized cellular architecture enabling efficient gas exchange. Surprisingly, the developmental processes underlying gill formation in zebrafish remain poorly understood. Here, we present for the first time a comprehensive analysis of the morphogenesis of gill arteries, filaments and lamellae during lifelong development. Our results provide important insights into the temporal and spatial pattern of gill angiogenesis, revealing fundamental differences in the formation of lateral versus medial filaments along the dorso-ventral axis. These early asymmetries correlate with, and likely underlie, the structural asymmetries observed in adult gills, which we quantitatively characterize. This indicates that a region-specific developmental programme establishes a blueprint for gill architecture maintained throughout life. We further show that lamellae develop through a complex interplay between endothelial and cranial neural crest-derived pillar cells. Notably, lamellar size, which strongly influences respiratory efficiency, depends on the position of the filament in the arch. Together, our work identifies key cellular and temporal mechanisms driving gill development, and provides a framework to investigate broader principles of branching morphogenesis and angiogenesis in vertebrates.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12517346/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144946992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dendritic atoh1a+ cells serve as Merkel cell precursors during skin development and regeneration. 树突状atoh1a+细胞在皮肤发育和再生过程中作为默克尔细胞的前体。

IF 3.7 2区生物学

Development Pub Date : 2025-10-15 Epub Date: 2025-06-20 DOI: 10.1242/dev.204810

Evan W Craig, Erik C Black, Samantha Z Fernandes, Ahlan S Ferdous, Camille E A Goo, Sheridan M Sargent, Elgene J A Quitevis, Avery Angell Swearer, Nathaniel G Yee, Jimann Shin, Lilianna Solnica-Krezel, Jeffrey P Rasmussen

{"title":"Dendritic atoh1a+ cells serve as Merkel cell precursors during skin development and regeneration.","authors":"Evan W Craig, Erik C Black, Samantha Z Fernandes, Ahlan S Ferdous, Camille E A Goo, Sheridan M Sargent, Elgene J A Quitevis, Avery Angell Swearer, Nathaniel G Yee, Jimann Shin, Lilianna Solnica-Krezel, Jeffrey P Rasmussen","doi":"10.1242/dev.204810","DOIUrl":"10.1242/dev.204810","url":null,"abstract":"Sensory cells often adopt specific morphologies that aid in the detection of external stimuli. Merkel cells encode gentle touch stimuli in vertebrate skin and adopt a reproducible shape characterized by spiky actin-rich microvilli that emanate from the cell surface. The mechanisms by which Merkel cells acquire this stereotyped morphology from keratinocyte progenitors are unknown. Here, we establish that dendritic Merkel cells (dMCs) express atonal homolog 1a (atoh1a), extend dynamic filopodial processes, and arise in transient waves during zebrafish skin development and regeneration. We find that dMCs share molecular similarities with both basal keratinocytes and Merkel cells, yet display mesenchymal-like behaviors, including local cell motility and proliferation within the epidermis. Furthermore, dMCs can directly adopt the mature, microvilliated Merkel cell morphology through substantial remodeling of the actin cytoskeleton. Loss of Ectodysplasin A signaling alters the morphology of dMCs and Merkel cells within specific skin regions. Our results show that dMCs represent an intermediate state in the Merkel cell maturation program and identify Ectodysplasin A signaling as a key regulator of Merkel cell morphology.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12212647/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144186792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The transcription factor LHX2 suppresses astrocyte proliferation in the postnatal mammalian cerebral cortex. 转录因子LHX2抑制出生后哺乳动物大脑皮层星形胶质细胞增殖。

IF 3.7 2区生物学

Development Pub Date : 2025-10-15 Epub Date: 2025-06-02 DOI: 10.1242/dev.204358

Archana Iyer, Reanne Fronteiro, Prachi Bhatia, Sanjna Kumari, Amrita Singh, Jiafeng Zhou, Riccardo Bocchi, Rishikesh Narayanan, Shubha Tole

引用次数: 0

KLF7 orchestrates hippocampal development through neurogenesis and Draxin-mediated neuronal migration. KLF7通过神经发生和draxin介导的神经元迁移协调海马发育。

IF 3.6 2区生物学

Development Pub Date : 2025-10-15 Epub Date: 2025-08-05 DOI: 10.1242/dev.204718

Yitong Liu, Wentong Hong, Yuyan Zhou, Ailing Zhang, Pifang Gong, Guibo Qi, Xuan Song, Zhenru Wang, Xuanming Shi, Congcong Qi, Song Qin

{"title":"KLF7 orchestrates hippocampal development through neurogenesis and Draxin-mediated neuronal migration.","authors":"Yitong Liu, Wentong Hong, Yuyan Zhou, Ailing Zhang, Pifang Gong, Guibo Qi, Xuan Song, Zhenru Wang, Xuanming Shi, Congcong Qi, Song Qin","doi":"10.1242/dev.204718","DOIUrl":"10.1242/dev.204718","url":null,"abstract":"The hippocampus, a brain region that is crucial for cognitive learning, memory and emotional regulation, undergoes its primary development during embryonic and early postnatal stages. Krüppel-like factor 7 (KLF7), a transcription factor associated with autism spectrum disorder and intellectual developmental disorders, plays a pivotal role in brain development. In this study, we investigated the role of KLF7 in hippocampal development using conditional knockout mice [Emx1-Cre;Klf7Flox(F)/F]. We found that KLF7 deletion in hippocampal progenitors resulted in significant hippocampal shrinkage, disrupting neurogenesis, neuronal differentiation and migration. KLF7 mutant mice exhibited abnormal neuronal projections, anxiety- and depression-like behaviors, and memory impairments. Transcriptomic profiling identified Draxin, a neural chemorepellent, as a key downstream target of KLF7. Remarkably, overexpression of Draxin rescued dentate gyrus granule cell migration defects in KLF7 mutant mice. These findings demonstrate that KLF7 is essential for proper hippocampal development and function, regulating neuronal migration through Draxin. This study provides mechanistic insights into the neurological deficits associated with KLF7 pathogenic variants and highlights potential therapeutic targets for neurodevelopmental disorders.","PeriodicalId":11375,"journal":{"name":"Development","volume":"152 20","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12377812/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144783788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A single-cell transcriptomic atlas of sensory-dependent gene expression in developing mouse visual cortex. 发育中的小鼠视觉皮层中感觉依赖基因表达的单细胞转录组图谱。

IF 3.7 2区生物学

Development Pub Date : 2025-10-15 Epub Date: 2025-03-27 DOI: 10.1242/dev.204244

Andre M Xavier, Qianyu Lin, Chris J Kang, Lucas Cheadle

{"title":"A single-cell transcriptomic atlas of sensory-dependent gene expression in developing mouse visual cortex.","authors":"Andre M Xavier, Qianyu Lin, Chris J Kang, Lucas Cheadle","doi":"10.1242/dev.204244","DOIUrl":"10.1242/dev.204244","url":null,"abstract":"Sensory experience drives the maturation of neural circuits during postnatal brain development through molecular mechanisms that remain to be fully elucidated. One likely mechanism involves the sensory-dependent expression of genes that encode direct mediators of circuit remodeling within developing cells. To identify potential drivers of sensory-dependent synaptic development, we generated a single-nucleus RNA sequencing dataset describing the transcriptional responses of cells in the mouse visual cortex to sensory deprivation or to stimulation during a developmental window when visual input is necessary for circuit refinement. We sequenced 118,529 nuclei across 16 neuronal and non-neuronal cell types isolated from control, sensory deprived and sensory stimulated mice, identifying 1268 sensory-induced genes within the developing brain. While experience elicited transcriptomic changes in all cell types, excitatory neurons in layer 2/3 exhibited the most robust changes, and the sensory-induced genes in these cells are poised to strengthen synapse-to-nucleus crosstalk and to promote cell type-specific axon guidance pathways. Altogether, we expect this dataset to significantly broaden our understanding of the molecular mechanisms through which sensory experience shapes neural circuit wiring in the developing brain.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143523044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cited4a limits cardiomyocyte dedifferentiation and proliferation during zebrafish heart regeneration. Cited4a限制斑马鱼心脏再生过程中心肌细胞的去分化和增殖。

IF 3.6 2区生物学

Development Pub Date : 2025-10-15 Epub Date: 2025-08-19 DOI: 10.1242/dev.204659

Rachel Forman-Rubinsky, Angela Paul, Wei Feng, Brent T Schlegel, Daniel A Zuppo, Katarzyna Kedziora, Donna B Stoltz, Simon C Watkins, Dhivyaa Rajasundaram, Guang Li, Michael Tsang

{"title":"Cited4a limits cardiomyocyte dedifferentiation and proliferation during zebrafish heart regeneration.","authors":"Rachel Forman-Rubinsky, Angela Paul, Wei Feng, Brent T Schlegel, Daniel A Zuppo, Katarzyna Kedziora, Donna B Stoltz, Simon C Watkins, Dhivyaa Rajasundaram, Guang Li, Michael Tsang","doi":"10.1242/dev.204659","DOIUrl":"10.1242/dev.204659","url":null,"abstract":"Cardiac regeneration involves the interplay of complex interactions between many different cell types, including cardiomyocytes. In regeneration, cardiomyocytes undergo dedifferentiation and proliferation to replace lost cells at the injury border. The exact mechanism regulating this process is not completely understood. Here, we report a single nucleus RNA-sequencing profile of the injured zebrafish heart revealing distinct cardiomyocyte populations. These cardiomyocyte populations have diverse functions, including stress response, myofibril assembly, proliferation and contraction. Notably, increased expression of cited4a, a p300/CBP transcriptional coactivator, was detected in the mature contracting cardiomyocytes, but absent from proliferating cardiomyocytes, suggesting it may play a role in maintaining contractile function in a subset of cardiomyocytes. We reasoned that cited4a is induced in heart injury to maintain cardiomyocyte function and therefore cited4a+ populations are restricted from entering the cell cycle. Loss-of-function cited4a mutants were generated and, following ventricular resection, increased cardiomyocyte dedifferentiation and proliferation was observed. Our findings indicate that suppressing cited4a activity in the injured heart expands the pool of cardiomyocytes available for replacing damaged and lost myocardium and could be an approach to promote heart regeneration.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144689564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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