Development最新文献

Destabilisation of bam transcripts terminates the mitotic phase of Drosophila female germline differentiation.

IF 3.7 2区生物学

Development Pub Date : 2025-02-18 DOI: 10.1242/dev.204405

Tamsin J Samuels, Elizabeth J Torley, Valeriia Nadmitova, Emily L Naden, Phoebe E Blair, Frankjel A Hernandez Frometa, Felipe Karam Teixeira

{"title":"Destabilisation of bam transcripts terminates the mitotic phase of Drosophila female germline differentiation.","authors":"Tamsin J Samuels, Elizabeth J Torley, Valeriia Nadmitova, Emily L Naden, Phoebe E Blair, Frankjel A Hernandez Frometa, Felipe Karam Teixeira","doi":"10.1242/dev.204405","DOIUrl":"https://doi.org/10.1242/dev.204405","url":null,"abstract":"The tight control of the mitotic phase of differentiation is crucial to prevent tumourigenesis while securing tissue homeostasis. In the Drosophila female germline, differentiation involves precisely four mitotic divisions, and accumulating evidence suggests that bag-of-marbles (bam), the initiator of differentiation, is also involved in controlling the number of divisions. To test this hypothesis, we depleted Bam from differentiating cells and found a reduced number of mitotic divisions. We examined the regulation of Bam using RNA imaging methods and found that the bam 3' UTR conveys instability to the transcript in the 8-cell cyst and early 16-cell cyst. We show that the RNA binding protein, Rbp9, is responsible for timing bam mRNA decay. Rbp9 itself is part of a sequential cascade of RNA binding proteins activated downstream of Bam, and we show that it is regulated through a change in transcription start site, driven by Rbfox1. Altogether, we propose a model in which Bam expression at the dawn of differentiation initiates a series of events that eventually terminates the Bam expression domain.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143440303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Machine learning approaches for image classification in developmental biology and clinical embryology.

IF 3.7 2区生物学

Development Pub Date : 2025-02-15 Epub Date: 2025-02-17 DOI: 10.1242/dev.202066

Camilla Mapstone, Berenika Plusa

引用次数: 0

Dendritic cells in developing and adult zebrafish arise from different origins and display distinct flt3 dependencies.

IF 3.7 2区生物学

Development Pub Date : 2025-02-15 Epub Date: 2025-02-17 DOI: 10.1242/dev.204410

Guanzhen Lin, Youqi Wang, Thi Giang Pham, Zilong Wen

引用次数: 0

Autonomous function of Antennapedia in adult muscle precursors directly connects Hox genes to adult muscle development.

IF 3.7 2区生物学

Development Pub Date : 2025-02-15 Epub Date: 2025-02-17 DOI: 10.1242/dev.204341

Aakriti Singh, Meike van den Burgh, Vigneshwarr Boopathy, Patrick van Nierop Y Sanchez, Josephine Bageritz, Ingrid Lohmann, Katrin Domsch

{"title":"Autonomous function of Antennapedia in adult muscle precursors directly connects Hox genes to adult muscle development.","authors":"Aakriti Singh, Meike van den Burgh, Vigneshwarr Boopathy, Patrick van Nierop Y Sanchez, Josephine Bageritz, Ingrid Lohmann, Katrin Domsch","doi":"10.1242/dev.204341","DOIUrl":"10.1242/dev.204341","url":null,"abstract":"The evolutionarily conserved Hox genes define segment identities along the anterior-posterior axis and are expressed in most cell types within each segment, performing specific functions tailored to cellular needs. It has been suggested previously that Drosophila adult flight muscles in the second thoracic segment (T2) develop without direct Hox gene input, relying instead on ectodermal signals to shape their identity. However, our research, leveraging single-cell transcriptomics of Drosophila wing discs and Hox perturbation experiments using CRISPR technology and gain-of-function assays, unveiled a more intricate regulatory landscape. We found that the Hox protein Antennapedia (Antp) is essential for adult flight muscle development, acting in two crucial ways: by regulating the cell cycle rate of adult muscle precursors (AMPs) through repression of proliferation genes, and by guiding flight muscle fate via regulation of Hedgehog (Hh) signalling during cell fate establishment. Antp, along with its co-factor Apterous (Ap), directly interacts with the patched (ptc) locus to control its expression in AMPs. These findings challenge the notion of T2 as a 'Hox-free' zone, highlighting the indispensable role of low-level Antp expression in adult muscle development.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143370453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A cardiac transcriptional enhancer is repurposed during regeneration to activate an anti-proliferative program. 心脏转录增强子在再生过程中被重新利用以激活抗增殖程序。

IF 3.7 2区生物学

Development Pub Date : 2025-02-15 Epub Date: 2025-02-17 DOI: 10.1242/dev.204458

Anupama Rao, Andrew Russell, Jose Segura-Bermudez, Charles Franz, Rejenae Dockery, Anton Blatnik, Jacob Panten, Mateo Zevallos, Carson McNulty, Maciej Pietrzak, Joseph Aaron Goldman

{"title":"A cardiac transcriptional enhancer is repurposed during regeneration to activate an anti-proliferative program.","authors":"Anupama Rao, Andrew Russell, Jose Segura-Bermudez, Charles Franz, Rejenae Dockery, Anton Blatnik, Jacob Panten, Mateo Zevallos, Carson McNulty, Maciej Pietrzak, Joseph Aaron Goldman","doi":"10.1242/dev.204458","DOIUrl":"10.1242/dev.204458","url":null,"abstract":"Zebrafish have a high capacity to regenerate their hearts. Several studies have surveyed transcriptional enhancers to understand how gene expression is controlled during heart regeneration. We have identified REN (the runx1 enhancer) that, during regeneration, regulates the expression of the nearby runx1 gene. We show that runx1 mRNA is reduced with deletion of REN (ΔREN), and cardiomyocyte proliferation is enhanced in ΔREN mutants only during regeneration. Interestingly, in uninjured hearts, ΔREN mutants have reduced expression of adamts1, a nearby gene that encodes a Collagen protease. This results in excess Collagen within cardiac valves of uninjured hearts. The ΔREN Collagen phenotype is rescued by an allele with Δrunx1 mutations, suggesting that in uninjured hearts REN regulates adamts1 independently of runx1. Taken together, this suggests that REN is rewired from adamts1 in uninjured hearts to stimulate runx1 transcription during regeneration. Our data point to a previously unappreciated mechanism for gene regulation during zebrafish heart regeneration. We report that an enhancer is rewired from expression in a distal cardiac domain to activate a different gene in regenerating tissue.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142970054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dynamic map illuminates Hippo-cMyc module crosstalk driving cardiomyocyte proliferation.

IF 3.7 2区生物学

Development Pub Date : 2025-02-15 Epub Date: 2025-02-17 DOI: 10.1242/dev.204397

Bryana N Harris, Laura A Woo, R Noah Perry, Alexia M Wallace, Mete Civelek, Matthew J Wolf, Jeffrey J Saucerman

引用次数: 0

POU4F2/BRN3B overexpression promotes the genesis of retinal ganglion cell-like projection neurons from late progenitors.

IF 3.7 2区生物学

Development Pub Date : 2025-02-13 DOI: 10.1242/dev.204297

Viviane Medeiros Oliveira-Valença, Jacqueline Mary Roberts, Vitória Melo Fernandes-Cerqueira, Carolina Herkenhoff Colmerauer, Beatriz Cardoso de Toledo, Pedro Lucas Santos-França, Rafael Linden, Rodrigo Alves Portela Martins, Maurício Rocha-Martins, Alejandra Bosco, Monica Lynn Vetter, Mariana Souza da Silveira

{"title":"POU4F2/BRN3B overexpression promotes the genesis of retinal ganglion cell-like projection neurons from late progenitors.","authors":"Viviane Medeiros Oliveira-Valença, Jacqueline Mary Roberts, Vitória Melo Fernandes-Cerqueira, Carolina Herkenhoff Colmerauer, Beatriz Cardoso de Toledo, Pedro Lucas Santos-França, Rafael Linden, Rodrigo Alves Portela Martins, Maurício Rocha-Martins, Alejandra Bosco, Monica Lynn Vetter, Mariana Souza da Silveira","doi":"10.1242/dev.204297","DOIUrl":"https://doi.org/10.1242/dev.204297","url":null,"abstract":"Retinal ganglion cells (RGCs) are the projection neurons of the retina, and their death promotes an irreversible blindness. Several factors were described to control their genesis during retinal development which include Atoh7 as a major orchestrator for RGC program and downstream targets, including Pou4f factors, which in turn regulate key aspects of terminal differentiation. The absence of POU4F family genes results in defects in RGC differentiation, aberrant axonal elaboration and ultimately RGC death, confirming the requirement of POU4F factors for RGC development and survival, with a critical role in regulating RGC axon outgrowth and pathfinding. Here, we investigated in vivo whether ectopic Pou4f2 expression in late retinal progenitor cells (late RPCs) is sufficient to induce the generation of cells with RGC properties, including long range axon projections. We showed that Pou4f2 overexpression generates RGC-like cells that share morphological and transcriptional features with RGCs normally generated during early development and extend axonal projections up to the brain. In conclusion, these results showed that POU4F2 alone was sufficient to promote critical properties of projection neurons from retinal progenitors outside their developmental window.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143413505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Khdrbs1 drives redifferentiation of bipotential progenitor cell by inhibiting p53 in zebrafish biliary-mediated liver regeneration.

IF 3.7 2区生物学

Development Pub Date : 2025-02-13 DOI: 10.1242/dev.204266

Kai Gang, Qi Chen, Junhui Sun, Tingwei Zhang, Pengcheng Cai, Rui Ni, Jianlong Ma

{"title":"Khdrbs1 drives redifferentiation of bipotential progenitor cell by inhibiting p53 in zebrafish biliary-mediated liver regeneration.","authors":"Kai Gang, Qi Chen, Junhui Sun, Tingwei Zhang, Pengcheng Cai, Rui Ni, Jianlong Ma","doi":"10.1242/dev.204266","DOIUrl":"https://doi.org/10.1242/dev.204266","url":null,"abstract":"After severe liver injury, biliary epithelial cells (BECs) undergo dedifferentiation into bipotential progenitor cells (BPPCs), which subsequently redifferentiate into nascent hepatocytes and BECs to accomplish liver regeneration. However, the critical factors governing the redifferentiation process of BPPCs remain largely unknown. Here, using a zebrafish model of severe liver injury, we observed specific expression of khdrbs1a and khdrbs1b (collectively known as khdrbs1) in BPPCs through single-cell RNA analyses and fluorescence in situ hybridization. Subsequently, to eliminate the genetic compensation, we generated a CRISPR/dead Cas9-mediated system for interfering khdrbs1 in BECs, which caused the defective liver regeneration and impaired redifferentiation of BPPCs. Furthermore, the khdrbs1-/- mutant displayed impaired proliferation and redifferentiation of BPPCs during liver regeneration. Mechanistically, p53 signaling was activated in response to the loss of khdrbs1, and tp53 mutation partially rescued the defective liver regeneration of the khdrbs1-/- mutant. In summary, we elucidate that Khdrbs1 promotes the redifferentiation of BPPCs in part by inhibiting p53 activation during biliary-mediated liver regeneration in zebrafish.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143440304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multi-modal refinement of the human heart atlas during the first gestational trimester.

IF 3.7 2区生物学

Development Pub Date : 2025-02-10 DOI: 10.1242/dev.204555

Christopher De Bono, Yichi Xu, Samina Kausar, Marine Herbane, Camille Humbert, Sevda Rafatov, Chantal Missirian, Mathias Moreno, Weiyang Shi, Yorick Gitton, Alberto Lombardini, Ivo Vanzetta, Séverine Mazaud-Guittot, Alain Chédotal, Anaïs Baudot, Stéphane Zaffran, Heather C Etchevers

{"title":"Multi-modal refinement of the human heart atlas during the first gestational trimester.","authors":"Christopher De Bono, Yichi Xu, Samina Kausar, Marine Herbane, Camille Humbert, Sevda Rafatov, Chantal Missirian, Mathias Moreno, Weiyang Shi, Yorick Gitton, Alberto Lombardini, Ivo Vanzetta, Séverine Mazaud-Guittot, Alain Chédotal, Anaïs Baudot, Stéphane Zaffran, Heather C Etchevers","doi":"10.1242/dev.204555","DOIUrl":"https://doi.org/10.1242/dev.204555","url":null,"abstract":"Forty first-trimester human hearts were studied to lay groundwork for further studies of principles underlying congenital heart defects. We first sampled 49,227 cardiac nuclei from three fetuses at 8.6, 9.0, and 10.7 post-conceptional weeks (pcw) for single-nucleus RNA sequencing, enabling distinction of six classes comprising 21 cell types. Improved resolution led to identification of novel cardiomyocytes and minority autonomic and lymphatic endothelial transcriptomes, among others. After integration with 5-7 pcw heart single-cell RNAseq, we identified a human cardiomyofibroblast progenitor preceding diversification of cardiomyocyte and stromal lineages. Analysis of six Visium sections from two additional hearts was aided by deconvolution, and key spatial markers validated on sectioned and whole hearts in two- and three-dimensional space and over time. Altogether, anatomical-positional features including innervation, conduction and subdomains of the atrioventricular septum translate latent molecular identity into specialized cardiac functions. This atlas adds unprecedented spatial and temporal resolution to the characterization of human-specific aspects of early heart formation.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143381787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single cell-derived multicellular meristem: insights into male-to-hermaphrodite conversion and de novo meristem formation in Ceratopteris. 单细胞衍生的多细胞分生组织：角翅目雄性向雌雄同体转化和新生分生组织形成的见解。

IF 3.7 2区生物学

Development Pub Date : 2025-02-01 Epub Date: 2025-02-13 DOI: 10.1242/dev.204411

Xi Yang, An Yan, Xing Liu, Alexandria Volkening, Yun Zhou

{"title":"Single cell-derived multicellular meristem: insights into male-to-hermaphrodite conversion and de novo meristem formation in Ceratopteris.","authors":"Xi Yang, An Yan, Xing Liu, Alexandria Volkening, Yun Zhou","doi":"10.1242/dev.204411","DOIUrl":"10.1242/dev.204411","url":null,"abstract":"Land plants alternate between asexual sporophytes and sexual gametophytes. Unlike seed plants, ferns develop free-living gametophytes. Gametophytes of the model fern Ceratopteris exhibit two sex types: hermaphrodites with pluripotent meristems and males lacking meristems. In the absence of the pheromone antheridiogen, males convert to hermaphrodites by forming de novo meristems, although the mechanisms remain unclear. Using long-term time-lapse imaging and computational analyses, we captured male-to-hermaphrodite conversion at single-cell resolution and reconstructed the lineage and division atlas of newly formed meristems. Lineage tracing revealed that the de novo-formed meristem originates from a single non-antheridium cell: the meristem progenitor cell (MPC). During conversion, the MPC lineage showed increased mitotic activity, with marginal cells proliferating faster than inner cells. A mathematical model suggested that stochastic variation in cell division, combined with strong inhibitory signals from dividing marginal cells, is sufficient to explain gametophyte dynamics. Experimental disruption of division timing agreed with the model, showing that precise cell cycle progression is essential for MPC establishment and sex-type conversion. These findings reveal cellular mechanisms governing sex conversion and de novo meristem formation in land plants.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143001979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0