IF 3.7 2区生物学

Development Pub Date : 2024-10-15 Epub Date: 2024-08-09 DOI: 10.1242/dev.202610

Julien Leclercq, Jorge Torres-Paz, Maxime Policarpo, François Agnès, Sylvie Rétaux

{"title":"Evolution of the regulation of developmental gene expression in blind Mexican cavefish.","authors":"Julien Leclercq, Jorge Torres-Paz, Maxime Policarpo, François Agnès, Sylvie Rétaux","doi":"10.1242/dev.202610","DOIUrl":"10.1242/dev.202610","url":null,"abstract":"Developmental evolution and diversification of morphology can arise through changes in the regulation of gene expression or protein-coding sequence. To unravel mechanisms underlying early developmental evolution in cavefish of the species Astyanax mexicanus, we compared transcriptomes of surface-dwelling and blind cave-adapted morphs at the end of gastrulation. Twenty percent of the transcriptome was differentially expressed. Allelic expression ratios in cave X surface hybrids showed that cis-regulatory changes are the quasi-exclusive contributors to inter-morph variations in gene expression. Among a list of 108 genes with change at the cis-regulatory level, we explored the control of expression of rx3, which is a master eye gene. We discovered that cellular rx3 levels are cis-regulated in a cell-autonomous manner, whereas rx3 domain size depends on non-autonomous Wnt and Bmp signalling. These results highlight how uncoupled mechanisms and regulatory modules control developmental gene expression and shape morphological changes. Finally, a transcriptome-wide search for fixed coding mutations and differential exon use suggested that variations in coding sequence have a minor contribution. Thus, during early embryogenesis, changes in gene expression regulation are the main drivers of cavefish developmental evolution.","PeriodicalId":11375,"journal":{"name":"Development","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141616120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Topological analysis of 3D digital ovules identifies cellular patterns associated with ovule shape diversity. 三维数字胚珠拓扑分析确定了与胚珠形状多样性相关的细胞模式。

IF 4.6 2区生物学

Development Pub Date : 2024-10-15 Epub Date: 2024-05-30 DOI: 10.1242/dev.202590

Tejasvinee Atul Mody, Alexander Rolle, Nico Stucki, Fabian Roll, Ulrich Bauer, Kay Schneitz

{"title":"Topological analysis of 3D digital ovules identifies cellular patterns associated with ovule shape diversity.","authors":"Tejasvinee Atul Mody, Alexander Rolle, Nico Stucki, Fabian Roll, Ulrich Bauer, Kay Schneitz","doi":"10.1242/dev.202590","DOIUrl":"10.1242/dev.202590","url":null,"abstract":"Tissue morphogenesis remains poorly understood. In plants, a central problem is how the 3D cellular architecture of a developing organ contributes to its final shape. We address this question through a comparative analysis of ovule morphogenesis, taking advantage of the diversity in ovule shape across angiosperms. Here, we provide a 3D digital atlas of Cardamine hirsuta ovule development at single cell resolution and compare it with an equivalent atlas of Arabidopsis thaliana. We introduce nerve-based topological analysis as a tool for unbiased detection of differences in cellular architectures and corroborate identified topological differences between two homologous tissues by comparative morphometrics and visual inspection. We find that differences in topology, cell volume variation and tissue growth patterns in the sheet-like integuments and the bulbous chalaza are associated with differences in ovule curvature. In contrast, the radialized conical ovule primordia and nucelli exhibit similar shapes, despite differences in internal cellular topology and tissue growth patterns. Our results support the notion that the structural organization of a tissue is associated with its susceptibility to shape changes during evolutionary shifts in 3D cellular architecture.","PeriodicalId":11375,"journal":{"name":"Development","volume":null,"pages":null},"PeriodicalIF":4.6,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11168579/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140911018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The cellular basis of feeding-dependent body size plasticity in sea anemones. 海葵摄食依赖体型可塑性的细胞基础。

IF 3.7 2区生物学

Development Pub Date : 2024-10-15 Epub Date: 2024-07-09 DOI: 10.1242/dev.202926

Kathrin Garschall, Eudald Pascual-Carreras, Belén García-Pascual, Daria Filimonova, Annika Guse, Iain G Johnston, Patrick R H Steinmetz

{"title":"The cellular basis of feeding-dependent body size plasticity in sea anemones.","authors":"Kathrin Garschall, Eudald Pascual-Carreras, Belén García-Pascual, Daria Filimonova, Annika Guse, Iain G Johnston, Patrick R H Steinmetz","doi":"10.1242/dev.202926","DOIUrl":"10.1242/dev.202926","url":null,"abstract":"Many animals share a lifelong capacity to adapt their growth rates and body sizes to changing environmental food supplies. However, the cellular and molecular basis underlying this plasticity remains only poorly understood. We therefore studied how the sea anemones Nematostella vectensis and Aiptasia (Exaiptasia pallida) respond to feeding and starvation. Combining quantifications of body size and cell numbers with mathematical modelling, we observed that growth and shrinkage rates in Nematostella are exponential, stereotypic and accompanied by dramatic changes in cell numbers. Notably, shrinkage rates, but not growth rates, are independent of body size. In the facultatively symbiotic Aiptasia, we show that growth and cell proliferation rates are dependent on the symbiotic state. On a cellular level, we found that >7% of all cells in Nematostella juveniles reversibly shift between S/G2/M and G1/G0 cell cycle phases when fed or starved, respectively. Furthermore, we demonstrate that polyp growth and cell proliferation are dependent on TOR signalling during feeding. Altogether, we provide a benchmark and resource for further investigating the nutritional regulation of body plasticity on multiple scales using the genetic toolkit available for Nematostella.","PeriodicalId":11375,"journal":{"name":"Development","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11267454/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141558316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insights into the molecular bases of multicellular development from brown algae. 从褐藻中洞察多细胞发育的分子基础。

IF 3.7 2区生物学

Development Pub Date : 2024-10-15 Epub Date: 2024-09-20 DOI: 10.1242/dev.203004

Rita A Batista, Liping Wang, Kenny A Bogaert, Susana M Coelho

{"title":"Insights into the molecular bases of multicellular development from brown algae.","authors":"Rita A Batista, Liping Wang, Kenny A Bogaert, Susana M Coelho","doi":"10.1242/dev.203004","DOIUrl":"https://doi.org/10.1242/dev.203004","url":null,"abstract":"The transition from simple to complex multicellularity represents a major evolutionary step that occurred in only a few eukaryotic lineages. Comparative analyses of these lineages provide insights into the molecular and cellular mechanisms driving this transition, but limited understanding of the biology of some complex multicellular lineages, such as brown algae, has hampered progress. This Review explores how recent advances in genetic and genomic technologies now allow detailed investigations into the molecular bases of brown algae development. We highlight how forward genetic techniques have identified mutants that enhance our understanding of pattern formation and sexual differentiation in these organisms. Additionally, the existence and nature of morphogens in brown algae and the potential influence of the microbiome in key developmental processes are examined. Outstanding questions, such as the identity of master regulators, the definition and characterization of cell types, and the molecular bases of developmental plasticity are discussed, with insights into how recent technical advances could provide answers. Overall, this Review highlights how brown algae are emerging as alternative model organisms, contributing to our understanding of the evolution of multicellular life and the diversity of body plans.","PeriodicalId":11375,"journal":{"name":"Development","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142282058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extraordinary model systems for regeneration. 非凡的再生模型系统。

IF 3.7 2区生物学

Development Pub Date : 2024-10-15 Epub Date: 2024-07-15 DOI: 10.1242/dev.203083

Alice Accorsi, Longhua Guo, Wallace F Marshall, Mathilda T M Mommersteeg, Yu-Ichiro Nakajima

引用次数: 0

The role of TEAD4 in trophectoderm commitment and development is not conserved in non-rodent mammals. TEAD4在非啮齿类哺乳动物的滋养层外胚层承诺和发育中的作用并不保守。

IF 3.7 2区生物学

Development Pub Date : 2024-10-15 Epub Date: 2024-09-24 DOI: 10.1242/dev.202993

Alba Pérez-Gómez, Leopoldo González-Brusi, Inés Flores-Borobia, Beatriz Galiano-Cogolludo, Ismael Lamas-Toranzo, Julieta G Hamze, Adolfo Toledano-Díaz, Julián Santiago-Moreno, Priscila Ramos-Ibeas, Pablo Bermejo-Álvarez

{"title":"The role of TEAD4 in trophectoderm commitment and development is not conserved in non-rodent mammals.","authors":"Alba Pérez-Gómez, Leopoldo González-Brusi, Inés Flores-Borobia, Beatriz Galiano-Cogolludo, Ismael Lamas-Toranzo, Julieta G Hamze, Adolfo Toledano-Díaz, Julián Santiago-Moreno, Priscila Ramos-Ibeas, Pablo Bermejo-Álvarez","doi":"10.1242/dev.202993","DOIUrl":"10.1242/dev.202993","url":null,"abstract":"The first lineage differentiation in mammals gives rise to the inner cell mass and the trophectoderm (TE). In mice, TEAD4 is a master regulator of TE commitment, as it regulates the expression of other TE-specific genes and its ablation prevents blastocyst formation, but its role in other mammals remains unclear. Herein, we have observed that TEAD4 ablation in two phylogenetically distant species (bovine and rabbit) does not impede TE differentiation, blastocyst formation and the expression of TE markers, such as GATA3 and CDX2, although a reduced number of cells in the inner cell mass was observed in bovine TEAD4 knockout (KO) blastocysts. Transcriptional analysis in bovine blastocysts revealed no major transcriptional effect of the ablation, although the expression of hypoblast and Hippo signalling-related genes tended to be decreased in KO embryos. Experiments were conducted in the bovine model to determine whether TEAD4 was required for post-hatching development. TEAD4 KO spherical conceptuses showed normal development of the embryonic disc and TE, but hypoblast migration rate was reduced. At later stages of development (tubular conceptuses), no differences were observed between KO and wild-type conceptuses.","PeriodicalId":11375,"journal":{"name":"Development","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142016667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual role of BdMUTE during stomatal development in the model grass Brachypodium distachyon. BdMUTE 在模式草 Brachypodium distachyon 气孔发育过程中的双重作用。

IF 3.7 2区生物学

Development Pub Date : 2024-10-15 Epub Date: 2024-09-26 DOI: 10.1242/dev.203011

Roxane P Spiegelhalder, Lea S Berg, Tiago D G Nunes, Melanie Dörr, Barbara Jesenofsky, Heike Lindner, Michael T Raissig

{"title":"Dual role of BdMUTE during stomatal development in the model grass Brachypodium distachyon.","authors":"Roxane P Spiegelhalder, Lea S Berg, Tiago D G Nunes, Melanie Dörr, Barbara Jesenofsky, Heike Lindner, Michael T Raissig","doi":"10.1242/dev.203011","DOIUrl":"10.1242/dev.203011","url":null,"abstract":"Grasses form morphologically derived, four-celled stomata, where two dumbbell-shaped guard cells (GCs) are flanked by two lateral subsidiary cells (SCs). This innovative form enables rapid opening and closing kinetics and efficient plant-atmosphere gas exchange. The mobile bHLH transcription factor MUTE is required for SC formation in grasses. Yet whether and how MUTE also regulates GC development and whether MUTE mobility is required for SC recruitment is unclear. Here, we transgenically impaired BdMUTE mobility from GC to SC precursors in the emerging model grass Brachypodium distachyon. Our data indicate that reduced BdMUTE mobility severely affected the spatiotemporal coordination of GC and SC development. Furthermore, although BdMUTE has a cell-autonomous role in GC division orientation, complete dumbbell morphogenesis of GCs required SC recruitment. Finally, leaf-level gas exchange measurements showed that dosage-dependent complementation of the four-celled grass morphology was mirrored in a gradual physiological complementation of stomatal kinetics. Together, our work revealed a dual role of grass MUTE in regulating GC division orientation and SC recruitment, which in turn is required for GC morphogenesis and the rapid kinetics of grass stomata.","PeriodicalId":11375,"journal":{"name":"Development","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11449446/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142016665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microtubules and actin filaments direct nuclear movement during the polarisation of Marchantia spore cells. 微管和肌动蛋白丝在马氏囊孢子细胞极化过程中指导核运动。

IF 3.7 2区生物学

Development Pub Date : 2024-10-15 Epub Date: 2024-07-23 DOI: 10.1242/dev.202823

Sarah T Attrill, Hugh Mulvey, Clément Champion, Liam Dolan

{"title":"Microtubules and actin filaments direct nuclear movement during the polarisation of Marchantia spore cells.","authors":"Sarah T Attrill, Hugh Mulvey, Clément Champion, Liam Dolan","doi":"10.1242/dev.202823","DOIUrl":"https://doi.org/10.1242/dev.202823","url":null,"abstract":"The multicellular haploid stage of land plants develops from a single haploid cell produced by meiosis - the spore. Starting from a non-polar state, these spores develop polarity, divide asymmetrically and establish the first axis of symmetry. Here, we show that the nucleus migrates from the cell centroid to the basal pole during polarisation of the Marchantia polymorpha spore cell. A microtubule organising centre on the leading edge of the nucleus initiates a microtubule array between the nuclear surface and the cortex at the basal pole. Simultaneously, cortical microtubules disappear from the apical hemisphere but persist in the basal hemisphere. This is accompanied by the formation a dense network of fine actin filaments between the nucleus and the basal pole cortex. Experimental depolymerisation of either microtubules or actin filaments disrupts cellular asymmetry. These data demonstrate that the cytoskeleton reorganises during spore polarisation and controls the directed migration of the nucleus to the basal pole. The presence of the nucleus at the basal pole provides the cellular asymmetry for the asymmetric cell division that establishes the apical-basal axis of the plant.","PeriodicalId":11375,"journal":{"name":"Development","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141747660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adhesion and shrinkage transform the rounded pupal horn into an angular adult horn in Japanese rhinoceros beetle. 粘附和收缩将日本犀角金龟的圆形蛹角转化为角形成虫角。

IF 4.6 2区生物学

Development Pub Date : 2024-10-15 Epub Date: 2024-03-13 DOI: 10.1242/dev.202082

Keisuke Matsuda, Haruhiko Adachi, Hiroki Gotoh, Yasuhiro Inoue, Shigeru Kondo

引用次数: 0

Dissecting the dynamic cellular transcriptional atlas of adult teleost testis development throughout the annual reproductive cycle. 剖析成鱼睾丸在整个年度生殖周期中的动态细胞转录图谱。

IF 3.7 2区生物学

Development Pub Date : 2024-10-15 Epub Date: 2024-03-13 DOI: 10.1242/dev.202296

Chaofan Jin, Kai Yan, Mengya Wang, Weihao Song, Bo Wang, Yu Men, Jingjing Niu, Yan He, Quanqi Zhang, Jie Qi

{"title":"Dissecting the dynamic cellular transcriptional atlas of adult teleost testis development throughout the annual reproductive cycle.","authors":"Chaofan Jin, Kai Yan, Mengya Wang, Weihao Song, Bo Wang, Yu Men, Jingjing Niu, Yan He, Quanqi Zhang, Jie Qi","doi":"10.1242/dev.202296","DOIUrl":"10.1242/dev.202296","url":null,"abstract":"Teleost testis development during the annual cycle involves dramatic changes in cellular compositions and molecular events. In this study, the testicular cells derived from adult black rockfish at distinct stages - regressed, regenerating and differentiating - were meticulously dissected via single-cell transcriptome sequencing. A continuous developmental trajectory of spermatogenic cells, from spermatogonia to spermatids, was delineated, elucidating the molecular events involved in spermatogenesis. Subsequently, the dynamic regulation of gene expression associated with spermatogonia proliferation and differentiation was observed across spermatogonia subgroups and developmental stages. A bioenergetic transition from glycolysis to mitochondrial respiration of spermatogonia during the annual developmental cycle was demonstrated, and a deeper level of heterogeneity and molecular characteristics was revealed by re-clustering analysis. Additionally, the developmental trajectory of Sertoli cells was delineated, alongside the divergence of Leydig cells and macrophages. Moreover, the interaction network between testicular micro-environment somatic cells and spermatogenic cells was established. Overall, our study provides detailed information on both germ and somatic cells within teleost testes during the annual reproductive cycle, which lays the foundation for spermatogenesis regulation and germplasm preservation of endangered species.","PeriodicalId":11375,"journal":{"name":"Development","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140109573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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