Development最新文献

Spinal cord elongation enables proportional regulation of the zebrafish posterior body.

IF 3.7 2区生物学

Development Pub Date : 2025-01-02 DOI: 10.1242/dev.204438

Dillan Saunders, Carlos Camacho-Macorra, Benjamin Steventon

{"title":"Spinal cord elongation enables proportional regulation of the zebrafish posterior body.","authors":"Dillan Saunders, Carlos Camacho-Macorra, Benjamin Steventon","doi":"10.1242/dev.204438","DOIUrl":"https://doi.org/10.1242/dev.204438","url":null,"abstract":"Early embryos display a remarkable ability to regulate tissue patterning in response to changes in tissue size. However, it is not clear whether this ability continues into post-gastrulation stages. Here, we performed targeted removal of dorsal progenitors in the zebrafish tailbud using multiphoton ablation. This led to a proportional reduction in the length of the spinal cord and paraxial mesoderm in the tail, revealing a capacity for the regulation of tissue morphogenesis during tail formation. Following analysis of cell proliferation, gene expression, signalling and cell movements, we found no evidence of cell fate switching from mesoderm to neural fate to compensate for neural progenitor loss. Furthermore, tail paraxial mesoderm length is not reduced upon direct removal of an equivalent number of mesoderm progenitors, ruling out the hypothesis that neuromesodermal competent cells enable proportional regulation. Instead, reduction in cell number across the spinal cord reduces both spinal cord and paraxial mesoderm length. We conclude that spinal cord elongation is a driver of paraxial mesoderm elongation in the zebrafish tail and that this can explain proportional regulation upon neural progenitor reduction.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142913973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Positive autoregulation of Sox17 is necessary for gallbladder and extrahepatic biliary duct formation.

IF 3.7 2区生物学

Development Pub Date : 2025-01-02 DOI: 10.1242/dev.203033

Linh T Trinh, Ryan R Finnel, Anna B Osipovich, Jessica R Musselman, Leesa L Sampson, Christopher V E Wright, Mark A Magnuson

引用次数: 0

Abnormal H3K27me3 underlies degenerative spermatogonial stem cells in cryptorchid testis.

IF 3.7 2区生物学

Development Pub Date : 2025-01-02 DOI: 10.1242/dev.204239

Kazushige Kuroha, Ivana Dočkal, Uroš Radović, Kuniko Nakajima, Ikue Hoshi, Shion Matsuda, Noriko Kojitani, Kazuyuki Ohbo, Shin-Ichi Tomizawa

{"title":"Abnormal H3K27me3 underlies degenerative spermatogonial stem cells in cryptorchid testis.","authors":"Kazushige Kuroha, Ivana Dočkal, Uroš Radović, Kuniko Nakajima, Ikue Hoshi, Shion Matsuda, Noriko Kojitani, Kazuyuki Ohbo, Shin-Ichi Tomizawa","doi":"10.1242/dev.204239","DOIUrl":"https://doi.org/10.1242/dev.204239","url":null,"abstract":"Cryptorchidism is the most frequent congenital defect in newborn males characterized by the absence of the testis from the scrotum. Approximately 90% of patients with untreated bilateral cryptorchidism exhibit azoospermia due to defective spermatogenesis in the affected testis. While abnormal spermatogonial stem cell maintenance or differentiation is suggested to cause germ cell degeneration in the cryptorchid testis, underlying molecular mechanisms remain unclear. Here we profiled spermatogonial epigenetic landscapes using surgically induced cryptorchid testis in the mouse. We show that cryptorchidism leads to alterations in local, but not global H3K27me3 and H3K9me3 in undifferentiated spermatogonia. Of these, the loss of H3K27me3 was correlated with activation of developmental and proapoptotic pathway genes that are repressed by the polycomb machinery in germ cells. Cryptorchid spermatogonia exhibit the increase of H3K27me3 demethylases KDM6A and KMD6B. Furthermore, we reveal that an increased temperature leads to Kdm6a/b upregulation in germline stem cells cultured in vitro. Thus, our study suggests that temperature-dependent histone demethylation may induce mRNA dysregulation due to the partial loss of H3K27me3 in spermatogonia.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142913931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A master regulatory loop that activates genes in a temporally coordinated manner in muscle cells of ascidian embryos.

IF 3.7 2区生物学

Development Pub Date : 2025-01-02 DOI: 10.1242/dev.204382

Izumi Oda, Yutaka Satou

引用次数: 0

Her9 controls the stemness properties of hindbrain boundary cells.

IF 3.7 2区生物学

Development Pub Date : 2025-01-01 Epub Date: 2025-01-02 DOI: 10.1242/dev.203164

Carolyn Engel-Pizcueta, Covadonga F Hevia, Adrià Voltes, Jean Livet, Cristina Pujades

{"title":"Her9 controls the stemness properties of hindbrain boundary cells.","authors":"Carolyn Engel-Pizcueta, Covadonga F Hevia, Adrià Voltes, Jean Livet, Cristina Pujades","doi":"10.1242/dev.203164","DOIUrl":"10.1242/dev.203164","url":null,"abstract":"The different spatiotemporal distribution of progenitor and neurogenic capacities permits that brain regions engage asynchronously in neurogenesis. In the hindbrain, rhombomere progenitor cells contribute to neurons during the first neurogenic phase, whereas boundary cells participate later. To analyze what maintains boundary cells as non-neurogenic progenitors, we addressed the role of Her9, a zebrafish Hes1-related protein. her9 expression is temporarily sustained in boundary cells independently of Notch at early embryonic stages, while they are non-neurogenic progenitors. Complementary functional approaches show that Her9 inhibits the onset of Notch signaling and the neurogenic program, keeping boundary cells as progenitors. Multicolor clonal analysis combined with genetic perturbations reveal that Her9 expands boundary progenitors by promoting symmetric proliferative and preventing neurogenic cell divisions. Her9 also regulates the proliferation of boundary cells by inhibiting the cell cycle arrest gene cdkn1ca and interplaying with Cyclin D1. Moreover, her9 is enriched in hindbrain radial glial cells at late embryonic stages independently of Notch. Together these data demonstrate that Her9 maintains the stemness properties of hindbrain boundary progenitors and late radial glial cells, ensuring the different temporal distribution of neurogenic capacities within the hindbrain.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142766998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Licensing and niche competition in spermatogenesis: mathematical models suggest complementary regulation of tissue maintenance.

IF 3.7 2区生物学

Development Pub Date : 2025-01-01 Epub Date: 2025-01-02 DOI: 10.1242/dev.202796

Rodrigo García-Tejera, Jing-Yi Tian, Marc Amoyel, Ramon Grima, Linus J Schumacher

{"title":"Licensing and niche competition in spermatogenesis: mathematical models suggest complementary regulation of tissue maintenance.","authors":"Rodrigo García-Tejera, Jing-Yi Tian, Marc Amoyel, Ramon Grima, Linus J Schumacher","doi":"10.1242/dev.202796","DOIUrl":"https://doi.org/10.1242/dev.202796","url":null,"abstract":"To maintain and regenerate adult tissues after injury, division and differentiation of tissue-resident stem cells must be precisely regulated. It remains elusive which regulatory strategies prevent exhaustion or overgrowth of the stem cell pool, whether there is coordination between multiple mechanisms, and how to detect them from snapshots. In Drosophila testes, somatic stem cells transition to a state that licenses them to differentiate, but remain capable of returning to the niche and resuming cell division. Here, we build stochastic mathematical models for the somatic stem cell population to investigate how licensing contributes to homeostasis. We find that licensing, in combination with differentiation occurring in pairs, is sufficient to maintain homeostasis and prevent stem cell extinction from stochastic fluctuations. Experimental data have shown that stem cells are competing for niche access, and our mathematical models demonstrate that this contributes to the reduction in the variability of stem cell numbers but does not prevent extinction. Hence, a combination of both regulation strategies, licensing with pairwise differentiation and competition for niche access, may be needed to reduce variability and prevent extinction simultaneously.","PeriodicalId":11375,"journal":{"name":"Development","volume":"152 1","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142914014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Company of Biologists: celebrating 100 years.

IF 3.7 2区生物学

Development Pub Date : 2025-01-01 Epub Date: 2025-01-06 DOI: 10.1242/dev.204567

Sarah J Bray, Stephen J Royle, Holly A Shiels, Daniel St Johnston

引用次数: 0

Transitions in development - an interview with Maria Almuedo-Castillo.

IF 3.7 2区生物学

Development Pub Date : 2025-01-01 Epub Date: 2025-01-02 DOI: 10.1242/dev.204551

引用次数: 0

A comparative view of human and mouse telencephalon inhibitory neuron development.

IF 3.7 2区生物学

Development Pub Date : 2025-01-01 Epub Date: 2025-01-02 DOI: 10.1242/dev.204306

Changuk Chung, Joseph Girgiss, Joseph G Gleeson

引用次数: 0

Tango6 regulates HSPC proliferation and definitive haematopoiesis via Ikzf1 and Cmyb in caudal haematopoietic tissue.

IF 3.7 2区生物学

Development Pub Date : 2025-01-01 Epub Date: 2025-01-03 DOI: 10.1242/dev.202903

Shengnan Liu, Zhi Feng, Ming Su, Chenchen Liu, Yuan Xi, Huan Chen, Lingfei Luo, Xin Tian, Fangying Zhao, Li Li

{"title":"Tango6 regulates HSPC proliferation and definitive haematopoiesis via Ikzf1 and Cmyb in caudal haematopoietic tissue.","authors":"Shengnan Liu, Zhi Feng, Ming Su, Chenchen Liu, Yuan Xi, Huan Chen, Lingfei Luo, Xin Tian, Fangying Zhao, Li Li","doi":"10.1242/dev.202903","DOIUrl":"10.1242/dev.202903","url":null,"abstract":"Haematopoietic stem and progenitor cells (HSPCs) arise from the aorta-gonad-mesonephros and migrate to the caudal haematopoietic tissue (CHT) in zebrafish, where nascent HSPCs undergo tightly controlled proliferation and differentiation to promote definitive haematopoiesis. Effective expansion of HSPCs requires the coordination of well-established vesicle trafficking systems and appropriate transcription factors. However, the underlying molecules are yet to be identified. Using large-scale genetic screening of zebrafish larvae, Tango6 of the coat protein complex I (COPI) vesicle trafficking system was found to be indispensable for HSPC proliferation and definitive haematopoiesis. Homozygous tango6cq72 mutants display defective expansion of HSPCs in the CHT and compromised haematopoiesis. However, haematopoietic overexpression of Tango6 promoted haematopoietic expansion. tango6 deficiency caused a decline in RNA polymerase II subunit B and accumulation of DNA damage, which suppressed cell expansion in a P53-dependent manner. ikzf1 and cmyb (myb), two indispensable haematopoietic transcription factors, are targets of P53 and are used by tango6 in haematopoiesis. The haematopoietic phenotype was partially recovered by compensating for loss of ikzf1 and cmyb in tango6cq72 mutants. This study reveals a vesicle trafficking-mediated Tango6-P53-Ikzf1/Cmyb axis in zebrafish definitive haematopoiesis.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142767082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0