IF 3.7 2区生物学

Development Pub Date : 2025-10-15 Epub Date: 2025-07-09 DOI: 10.1242/dev.204483

Yuwei Pan, Shiyang Wang, Wuqi Yang, Xi Wu, Hanfu Zhang, Sujuan Du, Mingxin Zhang, Liyuan Hou, Maksim V Plikus, Jianwei Shuai, Cong Lv, Lu Yu, Zhengquan Yu

{"title":"Mesenchymal SLMAP coordinates with MST3 to govern gut elongation during development.","authors":"Yuwei Pan, Shiyang Wang, Wuqi Yang, Xi Wu, Hanfu Zhang, Sujuan Du, Mingxin Zhang, Liyuan Hou, Maksim V Plikus, Jianwei Shuai, Cong Lv, Lu Yu, Zhengquan Yu","doi":"10.1242/dev.204483","DOIUrl":"10.1242/dev.204483","url":null,"abstract":"Developing gut in mice undergoes rapid elongation during late embryogenesis, yet significantly slows down after birth. The precise regulatory mechanism of this dynamic morphogenetic process remains unknown. By utilizing single-cell RNA-sequencing analysis, we show that YAP activity in intestinal fibroblasts is the major molecular contributor to gut elongation. To determine how mesenchymal YAP activity is controlled, we identified canonical sarcolemma membrane-associated protein (SLMAP) as its critical regulator during mouse embryonic gut morphogenesis. Deleting Slmap in gut mesenchyme impairs YAP activity, leading to a short gut and a significant decrease in intestinal epithelial cell proliferation. Mechanistically, SLMAP activates YAP by directly regulating MST3 kinase. Physiologically, MST3 levels prominently increase over the developmental time, reaching their peak on postnatal day (P)14, when gut elongation in mice slows down. Depleting Mst3 in mesenchyme results in increased gut length at P14 accompanied by enhanced YAP activity. Importantly, a short gut phenotype in mesenchyme-specific Slmap mutant mice is partially compensated for by concomitant deletion of mesenchymal Mst3. Taken together, our findings demonstrate that SLMAP interacts with MST3 kinase to regulate the mesenchymal YAP activity that governs dynamic gut elongation across embryonic and postnatal development.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144301363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An interview with Mansi Srivastava. 对Mansi Srivastava的采访。

IF 3.7 2区生物学

Development Pub Date : 2025-10-15 Epub Date: 2025-06-17 DOI: 10.1242/dev.204952

引用次数: 0

Dendritic atoh1a+ cells serve as Merkel cell precursors during skin development and regeneration. 树突状atoh1a+细胞在皮肤发育和再生过程中作为默克尔细胞的前体。

IF 3.7 2区生物学

Development Pub Date : 2025-10-15 Epub Date: 2025-06-20 DOI: 10.1242/dev.204810

Evan W Craig, Erik C Black, Samantha Z Fernandes, Ahlan S Ferdous, Camille E A Goo, Sheridan M Sargent, Elgene J A Quitevis, Avery Angell Swearer, Nathaniel G Yee, Jimann Shin, Lilianna Solnica-Krezel, Jeffrey P Rasmussen

{"title":"Dendritic atoh1a+ cells serve as Merkel cell precursors during skin development and regeneration.","authors":"Evan W Craig, Erik C Black, Samantha Z Fernandes, Ahlan S Ferdous, Camille E A Goo, Sheridan M Sargent, Elgene J A Quitevis, Avery Angell Swearer, Nathaniel G Yee, Jimann Shin, Lilianna Solnica-Krezel, Jeffrey P Rasmussen","doi":"10.1242/dev.204810","DOIUrl":"10.1242/dev.204810","url":null,"abstract":"Sensory cells often adopt specific morphologies that aid in the detection of external stimuli. Merkel cells encode gentle touch stimuli in vertebrate skin and adopt a reproducible shape characterized by spiky actin-rich microvilli that emanate from the cell surface. The mechanisms by which Merkel cells acquire this stereotyped morphology from keratinocyte progenitors are unknown. Here, we establish that dendritic Merkel cells (dMCs) express atonal homolog 1a (atoh1a), extend dynamic filopodial processes, and arise in transient waves during zebrafish skin development and regeneration. We find that dMCs share molecular similarities with both basal keratinocytes and Merkel cells, yet display mesenchymal-like behaviors, including local cell motility and proliferation within the epidermis. Furthermore, dMCs can directly adopt the mature, microvilliated Merkel cell morphology through substantial remodeling of the actin cytoskeleton. Loss of Ectodysplasin A signaling alters the morphology of dMCs and Merkel cells within specific skin regions. Our results show that dMCs represent an intermediate state in the Merkel cell maturation program and identify Ectodysplasin A signaling as a key regulator of Merkel cell morphology.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12212647/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144186792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The transcription factor LHX2 suppresses astrocyte proliferation in the postnatal mammalian cerebral cortex. 转录因子LHX2抑制出生后哺乳动物大脑皮层星形胶质细胞增殖。

IF 3.7 2区生物学

Development Pub Date : 2025-10-15 Epub Date: 2025-06-02 DOI: 10.1242/dev.204358

Archana Iyer, Reanne Fronteiro, Prachi Bhatia, Sanjna Kumari, Amrita Singh, Jiafeng Zhou, Riccardo Bocchi, Rishikesh Narayanan, Shubha Tole

引用次数: 0

A single-cell transcriptomic atlas of sensory-dependent gene expression in developing mouse visual cortex. 发育中的小鼠视觉皮层中感觉依赖基因表达的单细胞转录组图谱。

IF 3.7 2区生物学

Development Pub Date : 2025-10-15 Epub Date: 2025-03-27 DOI: 10.1242/dev.204244

Andre M Xavier, Qianyu Lin, Chris J Kang, Lucas Cheadle

{"title":"A single-cell transcriptomic atlas of sensory-dependent gene expression in developing mouse visual cortex.","authors":"Andre M Xavier, Qianyu Lin, Chris J Kang, Lucas Cheadle","doi":"10.1242/dev.204244","DOIUrl":"10.1242/dev.204244","url":null,"abstract":"Sensory experience drives the maturation of neural circuits during postnatal brain development through molecular mechanisms that remain to be fully elucidated. One likely mechanism involves the sensory-dependent expression of genes that encode direct mediators of circuit remodeling within developing cells. To identify potential drivers of sensory-dependent synaptic development, we generated a single-nucleus RNA sequencing dataset describing the transcriptional responses of cells in the mouse visual cortex to sensory deprivation or to stimulation during a developmental window when visual input is necessary for circuit refinement. We sequenced 118,529 nuclei across 16 neuronal and non-neuronal cell types isolated from control, sensory deprived and sensory stimulated mice, identifying 1268 sensory-induced genes within the developing brain. While experience elicited transcriptomic changes in all cell types, excitatory neurons in layer 2/3 exhibited the most robust changes, and the sensory-induced genes in these cells are poised to strengthen synapse-to-nucleus crosstalk and to promote cell type-specific axon guidance pathways. Altogether, we expect this dataset to significantly broaden our understanding of the molecular mechanisms through which sensory experience shapes neural circuit wiring in the developing brain.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143523044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CSF1R ligands promote microglial proliferation but are not the sole regulators of developmental microglial proliferation. CSF-1R配体促进小胶质细胞增殖，但不是发育性小胶质细胞增殖的唯一调节因子。

IF 3.7 2区生物学

Development Pub Date : 2025-10-15 Epub Date: 2025-06-03 DOI: 10.1242/dev.204610

Brady P Hammond, Sameera Zia, Eugene Hahn, Margarita Kapustina, Tristan Lange, Sarah Friesen, Rupali Manek, Kelly V Lee, Adrian Castellanos-Molina, Floriane Bretheau, Mark S Cembrowski, Bradley J Kerr, Steve Lacroix, Jason R Plemel

{"title":"CSF1R ligands promote microglial proliferation but are not the sole regulators of developmental microglial proliferation.","authors":"Brady P Hammond, Sameera Zia, Eugene Hahn, Margarita Kapustina, Tristan Lange, Sarah Friesen, Rupali Manek, Kelly V Lee, Adrian Castellanos-Molina, Floriane Bretheau, Mark S Cembrowski, Bradley J Kerr, Steve Lacroix, Jason R Plemel","doi":"10.1242/dev.204610","DOIUrl":"10.1242/dev.204610","url":null,"abstract":"Microglia - the predominant immune cells of the brain and spinal cord - perform essential functions for the development and maintenance of the central nervous system, contingent upon the regulated developmental proliferation of microglia. However, the factor(s) that regulate microglial proliferation remain unclear. Here, we confirmed the timeline of developmental proliferation and used bioinformatics to identify potential signalling onto microglia in mouse from datasets collected at an age of high developmental microglial proliferation. Of the predicted factors, we found that colony stimulating factor 1 receptor (CSF1R) ligands boosted proliferation in vitro and were increasingly expressed in the brain across development with each displaying a distinct regional and temporal expression pattern. However, we did not observe a coincident alteration to CSF1R ligand levels in a model of abnormal developmental proliferation. Together, although CSF1R ligands can promote microglial proliferation in culture, their developmental expression patterns suggest that they function alongside other unknown factors to regulate developmental microglial proliferation.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12188242/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nr4a1 modulates inflammation and heart regeneration in zebrafish. Nr4a1调节斑马鱼的炎症和心脏再生。

IF 3.7 2区生物学

Development Pub Date : 2025-10-15 Epub Date: 2025-07-11 DOI: 10.1242/dev.204395

Dong Feng, Yanhan Dong, Yiran Song, Nicholas Yapundich, Yifang Xie, Brian Spurlock, Tingting Lyu, Landry Kuehn, Li Qian, Jiandong Liu

{"title":"Nr4a1 modulates inflammation and heart regeneration in zebrafish.","authors":"Dong Feng, Yanhan Dong, Yiran Song, Nicholas Yapundich, Yifang Xie, Brian Spurlock, Tingting Lyu, Landry Kuehn, Li Qian, Jiandong Liu","doi":"10.1242/dev.204395","DOIUrl":"10.1242/dev.204395","url":null,"abstract":"Recent findings have highlighted the complex role of inflammation in zebrafish heart regeneration, demonstrating that although inflammation is essential for initiating transient fibrosis and tissue repair, chronic inflammation, and unresolved fibrosis, could impede full regenerative recovery. In this study, we identified the nuclear receptor Nr4a1 as a crucial regulator of this regenerative process in zebrafish. Loss of Nr4a1 function led to a prolonged and excessive inflammatory response, disrupted neutrophil migration, delayed fibrin clearance, and ultimately impaired heart regeneration. Transcriptome-wide RNA-seq analysis at different injury stages revealed molecular disruptions associated with dysregulated inflammation and fibrosis in nr4a1 mutants. Notably, partial inhibition of the pro-inflammatory cytokine Tnfα rescued heart regeneration in the nr4a1 mutants, highlighting the therapeutic potential of modulating inflammation. Our findings suggest that Nr4a1 plays a crucial role in orchestrating the immune response during heart regeneration and may serve as a valuable target for enhancing cardiac repair following injury.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12276807/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144483600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single-cell transcriptomic profiling of the whole colony of Botrylloides diegensis: insights into tissue specialization and blastogenesis. 整个Botrylloides diegensis菌落的单细胞转录组学分析：对组织特化和胚发生的见解。

IF 3.7 2区生物学

Development Pub Date : 2025-10-15 Epub Date: 2025-06-17 DOI: 10.1242/dev.204265

Berivan Temiz, Michael Meier, Megan J Wilson

{"title":"Single-cell transcriptomic profiling of the whole colony of Botrylloides diegensis: insights into tissue specialization and blastogenesis.","authors":"Berivan Temiz, Michael Meier, Megan J Wilson","doi":"10.1242/dev.204265","DOIUrl":"10.1242/dev.204265","url":null,"abstract":"Botrylloides diegensis is a colonial ascidian that has been the focus of developmental, evolutionary and regeneration research. In this study, we performed single-cell RNA sequencing (scRNA-seq) of an entire B. diegensis colony, including zooids, buds and vascular tunics, to resolve cellular heterogeneity and to identify cell and tissue markers. We identified 29 major cell clusters within the colony and used in situ hybridization to examine the spatial expression of cluster marker genes. Numerous tissue types were identified at the molecular level, including blood cells and zooid tissues, such as the branchial epithelium, stomach and endostyle. Distinct cluster markers were identified for specific regions of the stomach epithelium, highlighting the specialization of these regions and the strength of using scRNA-seq to explore their functionality. Cell trajectory projections highlighted the early appearance of progenitor clusters, whereas more differentiated zooid-related tissues appeared later in the developmental path. This study provides a valuable resource for understanding the development, tissue function and regeneration of B. diegensis. It demonstrates the power of scRNA-seq to define cell types and tissues in complex colonial organisms.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12212465/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144126852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanisms underpinning spontaneous spinal cord regeneration. 支持自发脊髓再生的机制。

IF 3.6 2区生物学

Development Pub Date : 2025-10-15 Epub Date: 2025-07-30 DOI: 10.1242/dev.204790

Amruta Tendolkar, Mayssa H Mokalled

引用次数: 0

Pathway to Independence - an interview with Anzy Miller. 独立之路——对Anzy Miller的采访。

IF 3.6 2区生物学

Development Pub Date : 2025-08-01 Epub Date: 2025-08-04 DOI: 10.1242/dev.205038

引用次数: 0

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