Development最新文献_第10页

RFX6 regulates human intestinal patterning and function upstream of PDX1. RFX6 在 PDX1 的上游调控人类肠道模式和功能。

IF 3.7 2区生物学

Development Pub Date : 2024-09-15 Epub Date: 2024-09-20 DOI: 10.1242/dev.204379

J Guillermo Sanchez, Scott Rankin, Emily Paul, Heather A McCauley, Daniel O Kechele, Jacob R Enriquez, Nana-Hawa Jones, Siri A W Greeley, Lisa Letourneau-Freiberg, Aaron M Zorn, Mansa Krishnamurthy, James M Wells

引用次数: 0

The non-canonical bivalent gene Wfdc15a controls spermatogenic protease and immune homeostasis. 非经典双价基因Wfdc15a控制精子蛋白酶和免疫稳态。

IF 3.7 2区生物学

Development Pub Date : 2024-09-15 Epub Date: 2024-09-17 DOI: 10.1242/dev.202834

Shin-Ichi Tomizawa, Rachel Fellows, Michio Ono, Kazushige Kuroha, Ivana Dočkal, Yuki Kobayashi, Keisuke Minamizawa, Koji Natsume, Kuniko Nakajima, Ikue Hoshi, Shion Matsuda, Masahide Seki, Yutaka Suzuki, Kazushi Aoto, Hirotomo Saitsu, Kazuyuki Ohbo

{"title":"The non-canonical bivalent gene Wfdc15a controls spermatogenic protease and immune homeostasis.","authors":"Shin-Ichi Tomizawa, Rachel Fellows, Michio Ono, Kazushige Kuroha, Ivana Dočkal, Yuki Kobayashi, Keisuke Minamizawa, Koji Natsume, Kuniko Nakajima, Ikue Hoshi, Shion Matsuda, Masahide Seki, Yutaka Suzuki, Kazushi Aoto, Hirotomo Saitsu, Kazuyuki Ohbo","doi":"10.1242/dev.202834","DOIUrl":"10.1242/dev.202834","url":null,"abstract":"Male infertility can be caused by chromosomal abnormalities, mutations and epigenetic defects. Epigenetic modifiers pre-program hundreds of spermatogenic genes in spermatogonial stem cells (SSCs) for expression later in spermatids, but it remains mostly unclear whether and how those genes are involved in fertility. Here, we report that Wfdc15a, a WFDC family protease inhibitor pre-programmed by KMT2B, is essential for spermatogenesis. We found that Wfdc15a is a non-canonical bivalent gene carrying both H3K4me3 and facultative H3K9me3 in SSCs, but is later activated along with the loss of H3K9me3 and acquisition of H3K27ac during meiosis. We show that WFDC15A deficiency causes defective spermiogenesis at the beginning of spermatid elongation. Notably, depletion of WFDC15A causes substantial disturbance of the testicular protease-antiprotease network and leads to an orchitis-like inflammatory response associated with TNFα expression in round spermatids. Together, our results reveal a unique epigenetic program regulating innate immunity crucial for fertility.","PeriodicalId":11375,"journal":{"name":"Development","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142105438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tissue-specific RNA-seq defines genes governing male tail tip morphogenesis in C. elegans. 组织特异性RNA-seq确定了管理秀丽隐杆线虫雄性尾尖形态发生的基因。

IF 3.7 2区生物学

Development Pub Date : 2024-09-15 Epub Date: 2024-09-24 DOI: 10.1242/dev.202787

Karin C Kiontke, R Antonio Herrera, D Adam Mason, Alyssa Woronik, Stephanie Vernooy, Yash Patel, David H A Fitch

{"title":"Tissue-specific RNA-seq defines genes governing male tail tip morphogenesis in C. elegans.","authors":"Karin C Kiontke, R Antonio Herrera, D Adam Mason, Alyssa Woronik, Stephanie Vernooy, Yash Patel, David H A Fitch","doi":"10.1242/dev.202787","DOIUrl":"10.1242/dev.202787","url":null,"abstract":"Caenorhabditis elegans males undergo sex-specific tail tip morphogenesis (TTM) under the control of the DM-domain transcription factor DMD-3. To find genes regulated by DMD-3, we performed RNA-seq of laser-dissected tail tips. We identified 564 genes differentially expressed (DE) in wild-type males versus dmd-3(-) males and hermaphrodites. The transcription profile of dmd-3(-) tail tips is similar to that in hermaphrodites. For validation, we analyzed transcriptional reporters for 49 genes and found male-specific or male-biased expression for 26 genes. Only 11 DE genes overlapped with genes found in a previous RNAi screen for defective TTM. GO enrichment analysis of DE genes finds upregulation of genes within the unfolded protein response pathway and downregulation of genes involved in cuticle maintenance. Of the DE genes, 40 are transcription factors, indicating that the gene network downstream of DMD-3 is complex and potentially modular. We propose modules of genes that act together in TTM and are co-regulated by DMD-3, among them the chondroitin synthesis pathway and the hypertonic stress response.","PeriodicalId":11375,"journal":{"name":"Development","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11449441/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142282057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deep learning reveals a damage signalling hierarchy that coordinates different cell behaviours driving wound re-epithelialisation. 人工智能揭示了一种损伤信号层次结构，它能协调不同的细胞行为，推动伤口重新上皮化。

IF 3.7 2区生物学

Development Pub Date : 2024-09-15 Epub Date: 2024-09-24 DOI: 10.1242/dev.202943

Jake Turley, Francesca Robertson, Isaac V Chenchiah, Tanniemola B Liverpool, Helen Weavers, Paul Martin

{"title":"Deep learning reveals a damage signalling hierarchy that coordinates different cell behaviours driving wound re-epithelialisation.","authors":"Jake Turley, Francesca Robertson, Isaac V Chenchiah, Tanniemola B Liverpool, Helen Weavers, Paul Martin","doi":"10.1242/dev.202943","DOIUrl":"10.1242/dev.202943","url":null,"abstract":"One of the key tissue movements driving closure of a wound is re-epithelialisation. Earlier wound healing studies describe the dynamic cell behaviours that contribute to wound re-epithelialisation, including cell division, cell shape changes and cell migration, as well as the signals that might regulate these cell behaviours. Here, we have used a series of deep learning tools to quantify the contributions of each of these cell behaviours from movies of repairing wounds in the Drosophila pupal wing epithelium. We test how each is altered after knockdown of the conserved wound repair signals Ca2+ and JNK, as well as after ablation of macrophages that supply growth factor signals believed to orchestrate aspects of the repair process. Our genetic perturbation experiments provide quantifiable insights regarding how these wound signals impact cell behaviours. We find that Ca2+ signalling is a master regulator required for all contributing cell behaviours; JNK signalling primarily drives cell shape changes and divisions, whereas signals from macrophages largely regulate cell migration and proliferation. Our studies show deep learning to be a valuable tool for unravelling complex signalling hierarchies underlying tissue repair.","PeriodicalId":11375,"journal":{"name":"Development","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11449448/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142035544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Developmental emergence of first- and higher-order thalamic neuron molecular identities. 丘脑一阶和高阶神经元分子特征的发育过程。

IF 3.7 2区生物学

Development Pub Date : 2024-09-15 Epub Date: 2024-09-30 DOI: 10.1242/dev.202764

Quentin Lo Giudice, Robin J Wagener, Philipp Abe, Laura Frangeul, Denis Jabaudon

{"title":"Developmental emergence of first- and higher-order thalamic neuron molecular identities.","authors":"Quentin Lo Giudice, Robin J Wagener, Philipp Abe, Laura Frangeul, Denis Jabaudon","doi":"10.1242/dev.202764","DOIUrl":"10.1242/dev.202764","url":null,"abstract":"The thalamus is organized into nuclei that have distinct input and output connectivities with the cortex. Whereas first-order (FO) nuclei - also called core nuclei - relay input from sensory organs on the body surface and project to primary cortical sensory areas, higher-order (HO) nuclei - matrix nuclei - instead receive their driver input from the cortex and project to secondary and associative areas within cortico-thalamo-cortical loops. Input-dependent processes have been shown to play a crucial role in the emergence of FO thalamic neuron identity from a ground-state HO neuron identity, yet how this identity emerges during development remains unknown. Here, using single-cell RNA sequencing of the developing mouse embryonic thalamus, we show that, although they are born together, HO neurons start differentiating earlier than FO neurons. Within the FO visual thalamus, postnatal peripheral input is crucial for the maturation of excitatory, but not inhibitory, neurons. Our findings reveal different differentiation tempos and input sensitivities of HO and FO neurons, and highlight neuron type-specific molecular differentiation programs in the developing thalamus.","PeriodicalId":11375,"journal":{"name":"Development","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11463969/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142343729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transitions in development - an interview with Margarete Diaz Cuadros. 发展中的过渡--采访 Margarete Diaz Cuadros。

IF 3.7 2区生物学

Development Pub Date : 2024-09-15 Epub Date: 2024-09-16 DOI: 10.1242/dev.204337

引用次数: 0

Cadherin adhesion complexes direct cell aggregation in the epithelial transition of Wnt-induced nephron progenitor cells. 在 Wnt 诱导的肾祖细胞上皮转化过程中，粘附素粘附复合物引导细胞聚集。

IF 3.7 2区生物学

Development Pub Date : 2024-09-15 Epub Date: 2024-09-30 DOI: 10.1242/dev.202303

Balint Der, Helena Bugacov, Bohdana-Myroslava Briantseva, Andrew P McMahon

{"title":"Cadherin adhesion complexes direct cell aggregation in the epithelial transition of Wnt-induced nephron progenitor cells.","authors":"Balint Der, Helena Bugacov, Bohdana-Myroslava Briantseva, Andrew P McMahon","doi":"10.1242/dev.202303","DOIUrl":"10.1242/dev.202303","url":null,"abstract":"In the developing mammalian kidney, nephron formation is initiated by a subset of nephron progenitor cells (NPCs). Wnt input activates a β-catenin (Ctnnb1)-driven, transcriptional nephrogenic program and the mesenchymal to epithelial transition (MET) of NPCs. Using an in vitro mouse NPC culture model, we observed that activation of the Wnt pathway results in the aggregation of induced NPCs, which is an initiating step in the MET program. Genetic removal showed aggregation was dependent on β-catenin. Modulating extracellular Ca2+ levels showed cell-cell contacts were Ca2+ dependent, suggesting a role for cadherin (Cdh)-directed cell adhesion. Molecular analysis identified Cdh2, Cdh4 and Cdh11 in NPCs, and the β-catenin directed upregulation of Cdh3 and Cdh4 accompanying the MET of induced NPCs. Mutational analysis of β-catenin supported a role for a Lef/Tcf-β-catenin-mediated transcriptional response in the cell aggregation process. Genetic removal of all four cadherins, and independent removal of α-catenin or of β-catenin-α-catenin interactions, abolished aggregation, but not the inductive response to Wnt pathway activation. These findings, and data in an accompanying article highlight the role of β-catenin in linking transcriptional programs to the morphogenesis of NPCs in mammalian nephrogenesis.","PeriodicalId":11375,"journal":{"name":"Development","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11463967/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142343728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The people behind the papers - Yasmine el Azhar and Ina Sonnen. 报纸背后的人--亚斯明-埃尔-阿扎尔和伊娜-索南。

IF 3.7 2区生物学

Development Pub Date : 2024-09-15 Epub Date: 2024-09-26 DOI: 10.1242/dev.204385

引用次数: 0

The people behind the papers - Jake Turley. 报纸背后的人--杰克-特利。

IF 3.7 2区生物学

Development Pub Date : 2024-09-15 Epub Date: 2024-09-24 DOI: 10.1242/dev.204372

引用次数: 0

Macrophages play a crucial role in vascular smooth muscle cell coverage. 巨噬细胞在覆盖血管平滑肌细胞方面发挥着至关重要的作用。

IF 3.7 2区生物学

Development Pub Date : 2024-09-15 Epub Date: 2024-09-17 DOI: 10.1242/dev.203080

Kenta Niimi, Jun Nakae, Yoshiaki Kubota, Shinobu Inagaki, Tatsuo Furuyama

{"title":"Macrophages play a crucial role in vascular smooth muscle cell coverage.","authors":"Kenta Niimi, Jun Nakae, Yoshiaki Kubota, Shinobu Inagaki, Tatsuo Furuyama","doi":"10.1242/dev.203080","DOIUrl":"10.1242/dev.203080","url":null,"abstract":"The microvascular system consists of two cell types: endothelial and mural (pericytes and vascular smooth muscle cells; VSMCs) cells. Communication between endothelial and mural cells plays a pivotal role in the maintenance of vascular homeostasis; however, in vivo molecular and cellular mechanisms underlying mural cell development remain unclear. In this study, we found that macrophages played a crucial role in TGFβ-dependent pericyte-to-VSMC differentiation during retinal vasculature development. In mice with constitutively active Foxo1 overexpression, substantial accumulation of TGFβ1-producing macrophages and pericytes around the angiogenic front region was observed. Additionally, the TGFβ-SMAD pathway was activated in pericytes adjacent to macrophages, resulting in excess ectopic α-smooth muscle actin-positive VSMCs. Furthermore, we identified endothelial SEMA3C as an attractant for macrophages. In vivo neutralization of SEMA3C rescued macrophage accumulation and ectopic VSMC phenotypes in the mice, as well as drug-induced macrophage depletion. Therefore, macrophages play an important physiological role in VSMC development via the FOXO1-SEMA3C pathway.","PeriodicalId":11375,"journal":{"name":"Development","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142016666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0