Development最新文献_第10页

Distinct requirements for PI3K isoforms p110α and p110δ for PIP3 synthesis in mouse oocytes and early embryos. 小鼠卵母细胞和早期胚胎中PIP3合成对PI3K亚型p110α和p110δ有不同的要求。

IF 3.7 2区生物学

Development Pub Date : 2025-03-15 Epub Date: 2025-03-26 DOI: 10.1242/dev.204398

Anne Bourdais, Patricia Viard, Jenny Bormann, Côme Sesboüé, Daniel Guerrier, Nicole Therville, Julie Guillermet-Guibert, John Carroll, Guillaume Halet

{"title":"Distinct requirements for PI3K isoforms p110α and p110δ for PIP3 synthesis in mouse oocytes and early embryos.","authors":"Anne Bourdais, Patricia Viard, Jenny Bormann, Côme Sesboüé, Daniel Guerrier, Nicole Therville, Julie Guillermet-Guibert, John Carroll, Guillaume Halet","doi":"10.1242/dev.204398","DOIUrl":"10.1242/dev.204398","url":null,"abstract":"The phosphoinositide 3-kinase (PI3K)/Akt pathway is thought to regulate key steps of mammalian oogenesis, such as dormant oocyte awakening during follicular activation, meiotic resumption and oocyte maturation. Supporting evidence is, however, indirect, as oocyte PI3K activation has never been formally demonstrated, and the PI3K isoforms involved have not been revealed. Here, we employed fluorescent PIP3 biosensors to characterize PI3K dynamics in mouse oocytes and we investigated the contribution of the PI3K isoform p110α by conditional genetic ablation. Prophase oocytes showed baseline PI3K/Akt activation that could be further stimulated by adding Kit ligand. Contrary to previous reports, maternal PI3K proved dispensable for oocyte maturation in vitro, yet it was required for PIP3 synthesis in early embryos. We further show that oocyte p110α is not essential for oogenesis and female fertility. Accordingly, our data suggest that Kit ligand activates isoform p110δ for PIP3 synthesis in oocytes. In contrast, constitutive PIP3 synthesis in early embryos is achieved by maternal p110α acting redundantly with p110δ. This study highlights the relevance of PIP3 biosensors in establishing the dynamics, mechanisms and roles of maternal PI3K signaling during mammalian oogenesis.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143467310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gonadal sex determination in vertebrates: rethinking established mechanisms. 脊椎动物性腺性别决定：重新思考已建立的机制。

IF 3.7 2区生物学

Development Pub Date : 2025-03-15 Epub Date: 2025-03-31 DOI: 10.1242/dev.204592

Dagmar Wilhelm, Aitana Perea-Gomez, Axel Newton, Marie-Christine Chaboissier

引用次数: 0

Multi-modal refinement of the human heart atlas during the first gestational trimester. 妊娠早期人类心脏图谱的多模态改进。

IF 3.7 2区生物学

Development Pub Date : 2025-03-01 Epub Date: 2025-03-12 DOI: 10.1242/dev.204555

Christopher De Bono, Yichi Xu, Samina Kausar, Marine Herbane, Camille Humbert, Sevda Rafatov, Chantal Missirian, Mathias Moreno, Weiyang Shi, Yorick Gitton, Alberto Lombardini, Ivo Vanzetta, Séverine Mazaud-Guittot, Alain Chédotal, Anaïs Baudot, Stéphane Zaffran, Heather C Etchevers

{"title":"Multi-modal refinement of the human heart atlas during the first gestational trimester.","authors":"Christopher De Bono, Yichi Xu, Samina Kausar, Marine Herbane, Camille Humbert, Sevda Rafatov, Chantal Missirian, Mathias Moreno, Weiyang Shi, Yorick Gitton, Alberto Lombardini, Ivo Vanzetta, Séverine Mazaud-Guittot, Alain Chédotal, Anaïs Baudot, Stéphane Zaffran, Heather C Etchevers","doi":"10.1242/dev.204555","DOIUrl":"10.1242/dev.204555","url":null,"abstract":"Forty first-trimester human hearts were studied to lay groundwork for further studies of the mechanisms underlying congenital heart defects. We first sampled 49,227 cardiac nuclei from three fetuses at 8.6, 9.0, and 10.7 post-conceptional weeks (pcw) for single-nucleus RNA sequencing, enabling the distinction of six classes comprising 21 cell types. Improved resolution led to the identification of previously unappreciated cardiomyocyte populations and minority autonomic and lymphatic endothelial transcriptomes, among others. After integration with 5-7 pcw heart single-cell RNA-sequencing data, we identified a human cardiomyofibroblast progenitor preceding the diversification of cardiomyocyte and stromal lineages. Spatial transcriptomic analysis (six Visium sections from two additional hearts) was aided by deconvolution, and key spatial markers validated on sectioned and whole hearts in two- and three-dimensional space and over time. Altogether, anatomical-positional features, including innervation, conduction and subdomains of the atrioventricular septum, translate latent molecular identity into specialized cardiac functions. This atlas adds unprecedented spatial and temporal resolution to the characterization of human-specific aspects of early heart formation.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143381787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extra-embryonic mesoderm during development and in in vitro models. 发育过程中的胚外中胚层和体外模型。

IF 3.7 2区生物学

Development Pub Date : 2025-03-01 Epub Date: 2025-03-14 DOI: 10.1242/dev.204624

Eliana Nehme, Amitesh Panda, Isabelle Migeotte, Vincent Pasque

引用次数: 0

The people behind the papers - Juan Yang and Xuanmao Chen. 这些文件背后的人——杨娟和陈玄茂。

IF 3.7 2区生物学

Development Pub Date : 2025-03-01 Epub Date: 2025-03-11 DOI: 10.1242/dev.204728

引用次数: 0

Lepidopteran scale cells derive from sensory organ precursors through a canonical lineage. 鳞翅目鳞片细胞起源于感觉器官前体。

IF 3.7 2区生物学

Development Pub Date : 2025-03-01 Epub Date: 2025-03-07 DOI: 10.1242/dev.204501

Ling S Loh, Kyle A DeMarr, Martina Tsimba, Christa Heryanto, Alejandro Berrio, Nipam H Patel, Arnaud Martin, W Owen McMillan, Gregory A Wray, Joseph J Hanly

{"title":"Lepidopteran scale cells derive from sensory organ precursors through a canonical lineage.","authors":"Ling S Loh, Kyle A DeMarr, Martina Tsimba, Christa Heryanto, Alejandro Berrio, Nipam H Patel, Arnaud Martin, W Owen McMillan, Gregory A Wray, Joseph J Hanly","doi":"10.1242/dev.204501","DOIUrl":"10.1242/dev.204501","url":null,"abstract":"The success of butterflies and moths is tightly linked to the origin of scales within the group. A long-standing hypothesis postulates that scales are homologous to the well-described mechanosensory bristles found in the fruit fly Drosophila melanogaster, as both derive from an epithelial precursor. Previous histological and candidate gene approaches identified parallels in genes involved in scale and bristle development. Here, we provide developmental and transcriptomic evidence that the differentiation of lepidopteran scales derives from the sensory organ precursor (SOP). Live imaging in lepidopteran pupae shows that SOP cells undergo two asymmetric divisions that first abrogate the neurogenic lineage, and then lead to a differentiated scale precursor and its associated socket cell. Single-nucleus RNA sequencing using early pupal wings revealed differential gene expression patterns that mirror SOP development, suggesting a shared developmental program. Additionally, we recovered a newly associated gene, the transcription factor pdm3, involved in the proper differentiation of butterfly wing scales. Altogether, these data open up avenues for understanding scale type specification and development, and illustrate how single-cell transcriptomics provide a powerful platform for understanding evolution of cell types.","PeriodicalId":11375,"journal":{"name":"Development","volume":"152 5","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11925400/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143572540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Destabilisation of bam transcripts terminates the mitotic phase of Drosophila female germline differentiation. bam转录本的不稳定终止了果蝇雌性种系分化的有丝分裂期。

IF 3.7 2区生物学

Development Pub Date : 2025-03-01 Epub Date: 2025-03-11 DOI: 10.1242/dev.204405

Tamsin J Samuels, Elizabeth J Torley, Valeriia Nadmitova, Emily L Naden, Phoebe E Blair, Frankjel A Hernandez Frometa, Felipe Karam Teixeira

{"title":"Destabilisation of bam transcripts terminates the mitotic phase of Drosophila female germline differentiation.","authors":"Tamsin J Samuels, Elizabeth J Torley, Valeriia Nadmitova, Emily L Naden, Phoebe E Blair, Frankjel A Hernandez Frometa, Felipe Karam Teixeira","doi":"10.1242/dev.204405","DOIUrl":"10.1242/dev.204405","url":null,"abstract":"The tight control of the mitotic phase of differentiation is crucial to prevent tumourigenesis while securing tissue homeostasis. In the Drosophila female germline, differentiation involves precisely four mitotic divisions, and accumulating evidence suggests that bag of marbles (bam), the initiator of differentiation, is also involved in controlling the number of divisions. To test this hypothesis, we depleted Bam from differentiating cells and found a reduced number of mitotic divisions. We examined the regulation of Bam using RNA imaging methods and found that the bam 3' UTR conveys instability to the transcript in the eight-cell cyst and early 16-cell cyst. We show that the RNA-binding protein Rbp9 is responsible for timing bam mRNA decay. Rbp9 itself is part of a sequential cascade of RNA-binding proteins activated downstream of Bam, and we show that it is regulated through a change in transcription start site, driven by Rbfox1. Altogether, we propose a model in which Bam expression at the beginning of differentiation initiates a series of events that eventually terminates the Bam expression domain.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143440303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cilia directionality reveals a slow reverse movement of principal neurons for positioning and lamina refinement in the cerebral cortex. 纤毛方向性揭示了大脑皮层中主要神经元的缓慢反向运动，用于定位和层状细化。

IF 3.7 2区生物学

Development Pub Date : 2025-03-01 Epub Date: 2025-03-11 DOI: 10.1242/dev.204300

Juan Yang, Soheila Mirhosseiniardakani, Liyan Qiu, Kostandina Bicja, Abigail Del Greco, Kevin JungKai Lin, Mark Lyon, Xuanmao Chen

{"title":"Cilia directionality reveals a slow reverse movement of principal neurons for positioning and lamina refinement in the cerebral cortex.","authors":"Juan Yang, Soheila Mirhosseiniardakani, Liyan Qiu, Kostandina Bicja, Abigail Del Greco, Kevin JungKai Lin, Mark Lyon, Xuanmao Chen","doi":"10.1242/dev.204300","DOIUrl":"10.1242/dev.204300","url":null,"abstract":"Currently, not much is known about neuronal positioning and the roles of primary cilia in postnatal neurodevelopment. We show that primary cilia of principal neurons undergo marked changes in positioning and orientation, concurrent with postnatal neuron positioning in the mouse cerebral cortex. Primary cilia of early- and late-born principal neurons in compact layers display opposite orientations, while neuronal primary cilia in loose laminae are predominantly oriented toward the pia. In contrast, astrocytes and interneurons, and neurons in nucleated brain regions do not display specific cilia directionality. We further discovered that the cell bodies of principal neurons in inside-out laminated regions spanning from the hippocampal CA1 region to neocortex undergo a slow 'reverse movement' for postnatal positioning and lamina refinement. Furthermore, selective disruption of cilia function in the forebrain leads to altered lamination and gyrification in the retrosplenial cortex that is formed by reverse movement. Collectively, this study identifies reverse movement as a fundamental process for principal cell positioning that refines lamination in the cerebral cortex and casts light on the evolutionary transition from three-layered allocortices to six-layered neocortices.","PeriodicalId":11375,"journal":{"name":"Development","volume":"152 5","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12050088/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143604180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

POU4F2 overexpression promotes the genesis of retinal ganglion cell-like projection neurons from late progenitors. POU4F2/BRN3B过表达促进视网膜神经节细胞样投射神经元的发生。

IF 3.7 2区生物学

Development Pub Date : 2025-03-01 Epub Date: 2025-03-03 DOI: 10.1242/dev.204297

Viviane Medeiros Oliveira-Valença, Jacqueline Marie Roberts, Vitória Melo Fernandes-Cerqueira, Carolina Herkenhoff Colmerauer, Beatriz Cardoso de Toledo, Pedro Lucas Santos-França, Rafael Linden, Rodrigo Alves Portela Martins, Maurício Rocha-Martins, Alejandra Bosco, Monica Lynn Vetter, Mariana Souza da Silveira

{"title":"POU4F2 overexpression promotes the genesis of retinal ganglion cell-like projection neurons from late progenitors.","authors":"Viviane Medeiros Oliveira-Valença, Jacqueline Marie Roberts, Vitória Melo Fernandes-Cerqueira, Carolina Herkenhoff Colmerauer, Beatriz Cardoso de Toledo, Pedro Lucas Santos-França, Rafael Linden, Rodrigo Alves Portela Martins, Maurício Rocha-Martins, Alejandra Bosco, Monica Lynn Vetter, Mariana Souza da Silveira","doi":"10.1242/dev.204297","DOIUrl":"10.1242/dev.204297","url":null,"abstract":"Retinal ganglion cells (RGCs) are the projection neurons of the retina, and their death promotes an irreversible blindness. Several factors were described to control their genesis during retinal development. These include Atoh7, a major orchestrator of the RGC program, and downstream targets of this transcription factor, including Pou4f factors, that in turn regulate key aspects of terminal differentiation. The absence of POU4F family genes results in defects in RGC differentiation, aberrant axonal elaboration and, ultimately, RGC death. This confirms the requirement of POU4F factors for RGC development and survival, with a crucial role in regulating RGC axon outgrowth and pathfinding. Here, we have investigated in vivo whether ectopic Pou4f2 expression in late retinal progenitor cells (late RPCs) is sufficient to induce the generation of cells with RGC properties, including long-range axon projections. We show that Pou4f2 overexpression generates RGC-like cells that share morphological and transcriptional features with RGCs that are normally generated during early development and extend axonal projections up to the brain. In conclusion, these results show that POU4F2 alone is sufficient to promote the crucial properties of projection neurons that arise from retinal progenitors outside their developmental window.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143413505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The people behind the papers - Matthew Kourakis and William Smith. 这些文件背后的人——马修·库拉基斯和威廉·史密斯。

IF 3.7 2区生物学

Development Pub Date : 2025-03-01 Epub Date: 2025-03-13 DOI: 10.1242/dev.204736

引用次数: 0