Development最新文献_第9页

Application and measurement of tissue-scale tension in avian epithelia in vivo to study multiscale mechanics and inter-germ layer coupling. 组织尺度张力在禽上皮体内的应用和测量，研究多尺度力学和胚层间耦合。

IF 3.6 2区生物学

Development Pub Date : 2025-08-15 Epub Date: 2025-08-29 DOI: 10.1242/dev.204561

Panagiotis Oikonomou, Lisa Calvary, Helena C Cirne, Andreas E Welch, John F Durel, Olivia Powell, Kwantae Kim, Nandan L Nerurkar

{"title":"Application and measurement of tissue-scale tension in avian epithelia in vivo to study multiscale mechanics and inter-germ layer coupling.","authors":"Panagiotis Oikonomou, Lisa Calvary, Helena C Cirne, Andreas E Welch, John F Durel, Olivia Powell, Kwantae Kim, Nandan L Nerurkar","doi":"10.1242/dev.204561","DOIUrl":"10.1242/dev.204561","url":null,"abstract":"As cross-disciplinary approaches drawing from mechanics have increasingly influenced our understanding of morphogenesis, tools to measure and perturb physical aspects of embryogenesis have expanded as well. However, it remains a challenge to measure mechanical properties and apply exogenous tissue-scale forces in vivo, particularly for epithelia. Exploiting size and accessibility of the chick embryo, we describe a technique to apply and measure exogenous forces in the order of ∼1-100 µN to the endoderm. To demonstrate the utility of this approach, we performed several proof-of-concept experiments, revealing fundamental, yet unexpected, mechanical behaviors in the early embryo. This included heterogeneous single-cell mechanotypes within the endoderm, a complex non-cell autonomous mechanical role for actin, and tight mechanical coupling across germ layers. To illustrate the broader utility of this method, we expanded this approach to the ectoderm as well, where the mechanical behavior of neural plate cells was distinct from that of the endoderm. These findings provide basic insights into the mechanics of embryonic epithelia in vivo in the early avian embryo, and provide a useful tool for future investigations of how morphogenesis is influenced by mechanical factors.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12448309/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144820907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Supracellular actomyosin assemblies: master coordinators of development. 细胞上肌动球蛋白组合：发育的主要协调者。

IF 3.6 2区生物学

Development Pub Date : 2025-08-15 Epub Date: 2025-08-26 DOI: 10.1242/dev.204896

Katja Röper

{"title":"Supracellular actomyosin assemblies: master coordinators of development.","authors":"Katja Röper","doi":"10.1242/dev.204896","DOIUrl":"10.1242/dev.204896","url":null,"abstract":"Most movement in biological systems is driven by assemblies of actomyosin, be it in the form of sarcomeres in muscles or as actomyosin networks in non-muscle cells. Actomyosin has several key functions within epithelial cells, the cells that will form most of the organs of an animal during development. One such function is to support cellular shape through an actomyosin cortex just underneath the plasma membrane. In addition, actomyosin accumulates apically at adherens and tight junctions, supporting cell-cell adhesion and epithelial tightness. Evidence over recent years has shown that apical actomyosin can also organise into 'supracellular' networks that seemingly span many cells. These large-scale assemblies either form interlinked networks of apical-medial actomyosin just underneath the free apical plasma membrane or form linear actomyosin cables at the level of adherens junctions. Both types of supracellular assemblies appear to be conserved across evolution, though were characterised in Drosophila. In this Review, I discuss the formation of these supracellular structures, the tissues in which they are known to function during development, their functional roles, and the remaining unknowns regarding their components and potential emergent properties.","PeriodicalId":11375,"journal":{"name":"Development","volume":"152 16","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12448320/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144947079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The people behind the papers - Keiji Itoh and Sergei Sokol. 报纸背后的人，伊藤圭二和谢尔盖·索科尔。

IF 3.6 2区生物学

Development Pub Date : 2025-08-15 Epub Date: 2025-08-26 DOI: 10.1242/dev.205150

引用次数: 0

In preprints: primed for patterning - how epiblast cells encode the neural map in motion. 在预印本中：启动模式-外胚层细胞如何编码运动中的神经图谱。

IF 3.6 2区生物学

Development Pub Date : 2025-08-15 DOI: 10.1242/dev.205129

See Swee Tang, Laura Pellegrini

引用次数: 0

Mutual repression between Pax2 and Snail factors regulates the epithelial/mesenchymal state during intermediate mesoderm differentiation. Pax2和Snail因子相互抑制，调控中胚层分化过程中上皮/间充质状态。

IF 3.6 2区生物学

Development Pub Date : 2025-08-15 DOI: 10.1242/dev.204848

Juan M Fons, Oscar H Ocaña, M Angela Nieto

{"title":"Mutual repression between Pax2 and Snail factors regulates the epithelial/mesenchymal state during intermediate mesoderm differentiation.","authors":"Juan M Fons, Oscar H Ocaña, M Angela Nieto","doi":"10.1242/dev.204848","DOIUrl":"10.1242/dev.204848","url":null,"abstract":"The pronephros is the first renal structure in the embryo, arising by mesenchymal-to-epithelial transition of the intermediate mesoderm, where Pax2 induces epithelialization and differentiation of this mesenchyme. Here, we show in chick embryos that Snail1 is sufficient to keep the intermediate mesoderm in an undifferentiated state by directly repressing Pax2 transcription. Reciprocally, Pax2 is sufficient and necessary to induce mesenchymal-to-epithelial transition by directly repressing Snail1 expression. We also show that BMP7 acts as an epithelialization signal by downregulating Snail1 and upregulating Pax2 expression. This, together with the Snail1/Pax2 reciprocal repression, establishes a regulatory loop within the bi-stability domain, a dynamic region of the anteroposterior axis where opposing retinoic acid/fibroblast growth factor gradients converge, and which has been found to regulate differentiation of the neural tube and somites. In conclusion, we show that the antagonism between Snail1 and Pax2 determines the epithelial/mesenchymal state during the differentiation of the intermediate mesoderm and propose that the bi-stability domain extends to the intermediate mesoderm, synchronizing the differentiation of all tissues aligned along the mediolateral embryonic axis.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12401534/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pathway to Independence - an interview with Sonya Widen. 通往独立的道路——采访索尼娅·威格。

IF 3.6 2区生物学

Development Pub Date : 2025-08-15 DOI: 10.1242/dev.205117

引用次数: 0

The people behind the papers - Reagan Lamb and Salvatore Cherra. 报纸背后的人，里根·兰姆和塞尔瓦托·切拉。

IF 3.6 2区生物学

Development Pub Date : 2025-08-15 Epub Date: 2025-08-26 DOI: 10.1242/dev.205151

引用次数: 0

The people behind the papers - Zoe Weeks and Laura Moody. 报纸背后的人，佐伊·威克斯和劳拉·穆迪。

IF 3.6 2区生物学

Development Pub Date : 2025-08-15 Epub Date: 2025-08-26 DOI: 10.1242/dev.205147

引用次数: 0

IF 3.6 2区生物学

Development Pub Date : 2025-08-15 Epub Date: 2025-08-26 DOI: 10.1242/dev.204681

Keiji Itoh, Olga Ossipova, Sergei Y Sokol

{"title":"Myocardin-related transcription factor regulates actomyosin contractility and apical junction remodeling during vertebrate neural tube closure.","authors":"Keiji Itoh, Olga Ossipova, Sergei Y Sokol","doi":"10.1242/dev.204681","DOIUrl":"10.1242/dev.204681","url":null,"abstract":"Myocardin-related transcription factor A (Mrtfa), also known as megakaryoblastic leukemia protein (Mkl1/MAL), associates with serum response factor (Srf) to regulate transcription in response to actin dynamics; however, the functions of Mrtfa in vertebrate embryos remain largely unknown. Here, we show that Mrtfa is required for neural plate folding in early Xenopus embryos. Mrtfa knockdown reduced F-actin levels and inhibited apical constriction in the neural and non-neural ectoderm. By contrast, overexpression of constitutively active Mrtfa induced apical constriction in ectodermal cells via remodeling of tricellular junctions and junctional recruitment of Myosin II. We also identify potential Mrtfa target genes in embryonic ectoderm that encode actins and many regulators of actomyosin networks and junction remodeling. Our findings suggest a role for Mrtfa in the control of morphogenetic movements during neurulation. We propose that the regulation of actomyosin contractility is an essential cellular response to Mrtfa-dependent transcriptional activation.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12448316/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144759462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adhesion strength, cell packing density and cell surface buckling in pericellular matrix-mediated tissue cohesion. 细胞周围基质介导的组织内聚中的粘附强度、细胞堆积密度和细胞表面屈曲。

IF 3.6 2区生物学

Development Pub Date : 2025-08-15 Epub Date: 2025-08-26 DOI: 10.1242/dev.204663

Rudolf Winklbauer, Olivia Luu, Debanjan Barua, Martina Nagel, Yunyun Huang

{"title":"Adhesion strength, cell packing density and cell surface buckling in pericellular matrix-mediated tissue cohesion.","authors":"Rudolf Winklbauer, Olivia Luu, Debanjan Barua, Martina Nagel, Yunyun Huang","doi":"10.1242/dev.204663","DOIUrl":"10.1242/dev.204663","url":null,"abstract":"Pericellular matrix-mediated cell-cell adhesion in Xenopus gastrula tissues is characterized by a spectrum of narrow and wide cell contacts that alternate with the non-adhesive surfaces of the interstitial space. Here we show, first, that knockdown of a pericellular matrix adhesion molecule, fibronectin, diminishes contact abundance, and hence cell-packing density, without reducing adhesion strength. Second, we find that cell surfaces in gastrula tissues exhibit solid-like behavior in the form of buckling and crumpling, shape modifications that are typically seen in thin elastic films. We propose that both phenomena are explained by generic properties of the pericellular matrix: its compression and consequent stiffening by the interpenetration of matrix layers during adhesive contact formation. We argue that this renders part of the cell surface non-adhesive to form interstitial gaps, and both gap surfaces and contacts prone to buckling and crumpling in line with cell contractility fluctuations. In this elasto-capillary model of tissue cohesion, the size of the interstitial space is determined by the abundance of the pericellular matrix, not by adhesion strength.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12448319/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144793721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0