Development最新文献_第8页

The Drosophila pseudokinase Tribbles translocates to the fat body membrane in response to fasting to modulate insulin sensitivity. 果蝇假激酶Tribbles易位到脂肪体膜，响应禁食调节胰岛素敏感性。

IF 3.7 2区生物学

Development Pub Date : 2025-04-15 Epub Date: 2025-04-28 DOI: 10.1242/dev.204493

Zachary Fischer, Christopher Nauman, Shima Shayestehpour, Laramie Pence, Samuel Bouyain, Xiaolan Yao, Leonard L Dobens

{"title":"The Drosophila pseudokinase Tribbles translocates to the fat body membrane in response to fasting to modulate insulin sensitivity.","authors":"Zachary Fischer, Christopher Nauman, Shima Shayestehpour, Laramie Pence, Samuel Bouyain, Xiaolan Yao, Leonard L Dobens","doi":"10.1242/dev.204493","DOIUrl":"10.1242/dev.204493","url":null,"abstract":"The Drosophila pseudokinase Tribbles (Trbl) shares conserved functions with human TRIB3 to bind and inhibit Akt phosphorylation-activation by the Insulin Receptor (InR) to reduce insulin responses; consistent with this, increased levels of human TRIB3 are linked to type 2 diabetes. Here, we show that in fat body cells of well-fed Drosophila larvae, Trbl expression is low and predominantly in the nucleus while fasting or genetic reduction of insulin signaling resulted in increased Trbl expression and Trbl protein translocation to the plasma membrane. An E/G mutation in the Trbl pseudokinase kinase activation loop dominantly interfered with Trbl function leading to increased Akt activity, increased stability of Trbl substrates, including Trbl itself, and aberrant redistribution of Trbl multimers to the membrane. Several strategies designed to increase Akt activity were sufficient to translocate Trbl to the membrane, consistent with the notion that subcellular trafficking of Trbl to the fat body cell membrane acts a rheostat to reduce the strength of Akt-mediated insulin responses, counter to the InR, which has been shown to redistribute away from the membrane to modulate insulin signaling.","PeriodicalId":11375,"journal":{"name":"Development","volume":"152 8","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12070071/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143989524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The people behind the papers - Tanner Robertson and Anna Huttenlocher. 报纸背后的人，坦纳·罗伯逊和安娜·哈滕洛彻。

IF 3.7 2区生物学

Development Pub Date : 2025-04-15 Epub Date: 2025-04-28 DOI: 10.1242/dev.204831

引用次数: 0

Rac1 and Nectin3 are essential for planar cell polarity-directed axon guidance in the peripheral auditory system. 在外周听觉系统中，Rac1和Nectin3对平面细胞极性定向轴突的引导至关重要。

IF 3.7 2区生物学

Development Pub Date : 2025-04-15 Epub Date: 2025-04-24 DOI: 10.1242/dev.204423

Shaylyn Clancy, Nicholas Xie, Tess Eluvathingal Muttikkal, Jasmine Wang, Aray Adylkhan, Esha Fateh, Margaret Smith, Phillip Wilson, Matthew Smith, Arielle Hogan, Ann Sutherland, Xiaowei Lu

{"title":"Rac1 and Nectin3 are essential for planar cell polarity-directed axon guidance in the peripheral auditory system.","authors":"Shaylyn Clancy, Nicholas Xie, Tess Eluvathingal Muttikkal, Jasmine Wang, Aray Adylkhan, Esha Fateh, Margaret Smith, Phillip Wilson, Matthew Smith, Arielle Hogan, Ann Sutherland, Xiaowei Lu","doi":"10.1242/dev.204423","DOIUrl":"https://doi.org/10.1242/dev.204423","url":null,"abstract":"Spiral ganglion neurons (SGNs) carry sound information from the cochlea to the hindbrain, and innervate either inner or outer hair cells. Type II SGNs (SGNIIs) extend peripheral afferents towards outer hair cells, which make a characteristic 90° turn towards the cochlear base and innervate multiple outer hair cells. It has been shown that the planar cell polarity (PCP) pathway acts non-autonomously in the cochlear epithelium to guide SGNII peripheral afferent turning. However, the underlying mechanisms are unknown. Here, we show that PCP signaling regulates junctional localization of the small GTPase Rac1 and the cell adhesion molecule Nectin3 in mouse cochlear supporting cells (SCs), which serve as intermediate targets of SGNII peripheral afferents. Loss of Rac1 or Nectin3 partially phenocopied SGNII peripheral afferent turning defects in Vangl2 mutants. We present evidence that Rac1 plays a non-autonomous role in part by regulating the localization of core PCP proteins Vangl2 and Dvl3 at the SC-SC junctions, while Nectin3 likely serves a cell adhesion function to control SGNII afferent turning. Together, these experiments identify Nectin3 and Rac1 as regulators of PCP-directed SGNII axon guidance in the cochlea.","PeriodicalId":11375,"journal":{"name":"Development","volume":"152 8","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12045628/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143988019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transitions in development - an interview with Giulia Quattrocolo. 开发中的过渡——茱莉亚·奎特罗罗访谈

IF 3.7 2区生物学

Development Pub Date : 2025-04-15 Epub Date: 2025-04-11 DOI: 10.1242/dev.204795

引用次数: 0

The matrix glycoprotein Papilin maintains the haematopoietic progenitor pool in Drosophila lymph glands. 基质糖蛋白乳头蛋白维持果蝇淋巴腺中的造血祖细胞池。

IF 3.7 2区生物学

Development Pub Date : 2025-04-01 Epub Date: 2025-04-10 DOI: 10.1242/dev.204367

Jae-In Lee, Sumin Park, Hyunji Park, Youngbin Lee, JinYoung Park, Donghoon Lee, Moon Jong Kim, Kwang-Min Choe

{"title":"The matrix glycoprotein Papilin maintains the haematopoietic progenitor pool in Drosophila lymph glands.","authors":"Jae-In Lee, Sumin Park, Hyunji Park, Youngbin Lee, JinYoung Park, Donghoon Lee, Moon Jong Kim, Kwang-Min Choe","doi":"10.1242/dev.204367","DOIUrl":"10.1242/dev.204367","url":null,"abstract":"Differentiation of prohaemocytes, the precursors of Drosophila blood cells (haemocytes), and the release of haemocytes from the lymph gland, a major larval haematopoietic organ, are vital responses to wasp infestation or tissue degeneration. Although cells and extracellular matrix (ECM) in the lymph gland are known to play a crucial role in haemocyte differentiation, the underlying mechanisms remain unclear. Here, we show that the matrix glycoprotein Papilin (Ppn) is essential for maintaining the prohaemocyte population in lymph glands. In Ppn-depleted larvae, haemocyte differentiation increased with a reduction in the prohaemocyte-containing medullary zone, and lymph gland lobes dispersed prematurely. Ppn was synthesised by plasmatocytes, forming lamellae mainly in the medullary zone. Microbial infection or wasp infestation disrupted the Ppn meshwork within lymph glands. Ppn colocalised with collagen, laminin, nidogen and perlecan. Ppn depletion disrupted the ECM structure, including perlecan organisation. Phenotypes caused by Ppn depletion were partially rescued by perlecan overexpression or inactivation of the epidermal growth factor receptor pathway. Thus, Ppn is crucial for maintaining lymph gland architecture and regulating haemocyte differentiation, highlighting an intricate interaction between the ECM and signalling pathways in haematopoiesis.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12045604/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143646861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of shell field populations in gastropods and their autonomous specification mechanism independent of inter-quartet interactions. 腹足类壳场种群的特征及其独立于四重奏相互作用的自主规范机制。

IF 3.7 2区生物学

Development Pub Date : 2025-04-01 Epub Date: 2025-04-04 DOI: 10.1242/dev.204538

Supanat Phuangphong, Hiroki Yoshikawa, Yune Kojima, Hiroshi Wada, Yoshiaki Morino

{"title":"Characterization of shell field populations in gastropods and their autonomous specification mechanism independent of inter-quartet interactions.","authors":"Supanat Phuangphong, Hiroki Yoshikawa, Yune Kojima, Hiroshi Wada, Yoshiaki Morino","doi":"10.1242/dev.204538","DOIUrl":"10.1242/dev.204538","url":null,"abstract":"The embryonic shell field of mollusks appears during gastrulation on the dorsal ectoderm and later develops into the adult shell-secreting mantle. Although several lines of evidence have revealed that the shell field is exclusively derived from the second quartet (2q) of 16-cell embryos, it is generally believed that its fate is established only after receiving inductive signals from cells derived from other quartets, such as the invaginated endoderm. However, the induction hypothesis remains questionable due to limited experimental evidence and contradictory results. Here, we re-investigated the induction hypothesis for shell field specification in the limpet. We identified three cell populations within the developing shell field using two-color in situ hybridization and single-cell transcriptome analysis, each characterized by distinct effector and transcription factor genes. The specification of each population was examined in 2q blastomeres isolated from 16-cell embryos. Even without inter-quartet interactions, marker gene expression for each shell field population was detected in the 2q-derived partial embryos. We conclude that the early specification of shell field in 2q-derived cells occurs largely independently of interactions with other quartets.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143656455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deletion of hepcidin disrupts iron homeostasis and hematopoiesis in zebrafish embryogenesis. Hepcidin缺失破坏斑马鱼胚胎发生中的铁稳态和造血。

IF 3.7 2区生物学

Development Pub Date : 2025-04-01 Epub Date: 2025-04-04 DOI: 10.1242/dev.204307

Wenyi Yang, Mingjian Peng, Youquan Wang, Xiaowen Zhang, Wei Li, Xue Zhai, Zhichao Wu, Peng Hu, Liangbiao Chen

引用次数: 0

Insulin-like growth factor 2 as a driving force for exponential expansion and differentiation of the neonatal thymus. 胰岛素样生长因子2 （IGF2）作为新生儿胸腺指数扩张和分化的驱动力。

IF 3.7 2区生物学

Development Pub Date : 2025-04-01 Epub Date: 2025-04-10 DOI: 10.1242/dev.204347

Seung Woo Kang, Bryan R Helm, Yu Wang, Shiyun Xiao, Wen Zhang, Anusha Vasudev, Ken S Lau, Qi Liu, Ellen R Richie, Laura P Hale, Nancy R Manley

{"title":"Insulin-like growth factor 2 as a driving force for exponential expansion and differentiation of the neonatal thymus.","authors":"Seung Woo Kang, Bryan R Helm, Yu Wang, Shiyun Xiao, Wen Zhang, Anusha Vasudev, Ken S Lau, Qi Liu, Ellen R Richie, Laura P Hale, Nancy R Manley","doi":"10.1242/dev.204347","DOIUrl":"10.1242/dev.204347","url":null,"abstract":"Like all organs, the thymus grows in size and function rapidly during development, but this growth comes to a halt after birth. However, the molecular mechanisms behind such a transition in the thymus remain obscure. Using single-cell RNA sequencing (scRNA-seq) of the murine thymic stroma, we identified that major transcriptomic changes occur in the endothelium and mesenchyme across the transition to homeostasis. Differentially expressed gene and intercellular network analyses of temporally resolved scRNA-seq data revealed fibroblast-derived insulin-like growth factor 2 (IGF2) as a candidate driving neonatal thymic expansion. We demonstrated that IGF2 activity promotes a cortical thymic epithelial cell-specific proliferation and is tightly regulated at the thymic growth transition. Bulk RNA-seq of human thymi across the transition also revealed that IGF2 drives thymic expansion, suggesting an evolutionarily conserved role. Our study highlights the role of fibroblast-derived IGF2 in promoting cortical thymic epithelial cell proliferation and differentiation, resulting in early thymic expansion that is followed by downregulation to establish homeostasis.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12045631/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The people behind the papers - Ramya Ranganathan and Andrea Streit. 这些报纸背后的人——Ramya Ranganathan和Andrea Streit。

IF 3.7 2区生物学

Development Pub Date : 2025-04-01 Epub Date: 2025-04-11 DOI: 10.1242/dev.204801

引用次数: 0

The Company of Biologists' Workshops: supporting our community and inspiring new science. 生物学家工作坊的公司：支持我们的社区和启发新的科学。

IF 3.7 2区生物学

Development Pub Date : 2025-04-01 Epub Date: 2025-04-07 DOI: 10.1242/dev.204708

Saanjbati Adhikari

引用次数: 0