Development最新文献_第8页

EpiCure (Epithelial Curation): a versatile and handy tool for curation of epithelial segmentation. EpiCure（上皮细胞管理）：一个多功能和方便的工具，用于管理上皮细胞分割。

IF 4 2区生物学

Development Pub Date : 2026-09-01 Epub Date: 2026-09-03 DOI: 10.1242/dev.205701

Gaëlle Letort, Léo Valon, Arthur Michaut, Tom Cumming, Laura Xénard, Minh-Son Phan, Nicolas Dray, Curtis T Rueden, François Schweisguth, Jérôme Gros, Laure Bally-Cuif, Jean-Yves Tinevez, Romain Levayer

{"title":"EpiCure (Epithelial Curation): a versatile and handy tool for curation of epithelial segmentation.","authors":"Gaëlle Letort, Léo Valon, Arthur Michaut, Tom Cumming, Laura Xénard, Minh-Son Phan, Nicolas Dray, Curtis T Rueden, François Schweisguth, Jérôme Gros, Laure Bally-Cuif, Jean-Yves Tinevez, Romain Levayer","doi":"10.1242/dev.205701","DOIUrl":"10.1242/dev.205701","url":null,"abstract":"Despite advances in deep-learning and bioimage analysis, manual curation of segmented and tracking data is still required to extract accurate quantitative single cell temporal information from large tissue/embryo movies. However, very few tools address specifically this challenge. We present here EpiCure (Epithelial Curation), a versatile tool designed to streamline and accelerate manual curation of segmentation and tracking in 2D movies of large epithelial tissues. EpiCure uses temporal information and morphometric parameters to automatically identify segmentation and tracking errors and provides user-friendly tools to correct them. It focuses on ergonomics and offers visualization options to help navigate movies covering a large number of cells, speeding up the detection and curation of errors. EpiCure is highly interoperable, supports input from diverse segmentation tools and includes multiple export filters enabling seamless integration with downstream analysis pipelines. Using movies from several animal models, we highlight the importance of curating cell segmentation and tracking for accurate downstream analysis, and how EpiCure helps by extracting single cell dynamics and detecting cellular events in a large dataset.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148673231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SMAD6 is required for normal foregut development in humans and frogs. SMAD6是人类和青蛙正常前肠发育所必需的。

IF 4 2区生物学

Development Pub Date : 2026-09-01 Epub Date: 2026-09-10 DOI: 10.1242/dev.205236

Vivien Pagan, Scott A Rankin, Nicole A Edwards, Konrad Thorner, Shangqian Xie, Wendy K Chung, Yufeng Shen, Aaron M Zorn, James M Wells

{"title":"SMAD6 is required for normal foregut development in humans and frogs.","authors":"Vivien Pagan, Scott A Rankin, Nicole A Edwards, Konrad Thorner, Shangqian Xie, Wendy K Chung, Yufeng Shen, Aaron M Zorn, James M Wells","doi":"10.1242/dev.205236","DOIUrl":"10.1242/dev.205236","url":null,"abstract":"Esophageal atresia and tracheoesophageal fistula (EA/TEF) are congenital malformations of the foregut. We identified two distinct variants in the BMP/TGFβ repressor SMAD6 in two individuals exhibiting EA/TEF. We investigated the function of SMAD6 in tracheoesophageal development using two orthogonal approaches in Xenopus embryos, both resulting in foregut malformations similar to those observed in EA/TEF. We then used human pluripotent stem cell-derived foregut epithelium and mesenchyme to explore the separate roles of SMAD6 in these germ layers. CRISPR-mediated disruption of human SMAD6 caused an increase in BMP signaling in foregut epithelium and mesenchyme consistent with its role as a BMP repressor. Loss of SMAD6 caused patterning defects in both tissue types; SMAD6-/- endoderm showed increased expression of distal gut tube markers and SMAD6-/- mesenchyme showed increased expression of ventral and posterior markers, including markers of cardiac and liver mesenchyme lineages. Furthermore, SMAD6-/- mesenchyme had decreased ability to form CD31-positive endothelial cells. Our results demonstrate that SMAD6 is required for foregut development and that rare variants in this gene are likely causative for foregut malformations in EA/TEF.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148757622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pathway to Independence - an interview with Jessica Perochon. 独立之路——杰西卡·佩罗雄的访谈。

IF 4 2区生物学

Development Pub Date : 2026-09-01 DOI: 10.1242/dev.206026

引用次数: 0

ATGL-mediated lipid droplet lipolysis promotes collective cell migration in Drosophila. atgl介导的脂滴脂解促进果蝇的集体迁移。

IF 4 2区生物学

Development Pub Date : 2026-09-01 Epub Date: 2026-08-17 DOI: 10.1242/dev.205486

Israel J Wipf, Emma J Lowden, Michelle S Giedt, Julia R Dorale, Kennedy F Godfredsen, Tina L Tootle

{"title":"ATGL-mediated lipid droplet lipolysis promotes collective cell migration in Drosophila.","authors":"Israel J Wipf, Emma J Lowden, Michelle S Giedt, Julia R Dorale, Kennedy F Godfredsen, Tina L Tootle","doi":"10.1242/dev.205486","DOIUrl":"10.1242/dev.205486","url":null,"abstract":"Although lipid droplets (LDs), organelles central to lipid and energy homeostasis, are implicated in cancer cell migration, their roles during collective cell migration remain unknown. We use Drosophila border cell (BC) migration as an in vivo model of invasive, collective migration to dissect the roles of LDs and the conserved LD lipase Adipose Triglyceride Lipase (ATGL). LDs undergo dynamic changes and exhibit a polarized distribution during BC migration. Loss of ATGL increases LD volume, whereas BC ATGL overexpression depletes LDs. Loss, knockdown or overexpression of ATGL delay migration and impair delamination. Additionally, loss of ATGL disrupts BC mitochondria functions, including membrane potential. Similarly, loss of the mitochondrial fatty acid importer carnitine palmitoyltransferase 1 (CPT1) reduces mitochondrial membrane potential and severely blocks delamination. These results demonstrate that tight regulation of lipid mobilization from LDs, including its role in energy production, drives delamination and collective migration. These findings not only suggest how cancer cells may exploit LDs to promote their invasive behaviors but also highlight the crucial role of LDs in migration during development, hinting at their broader significance in diverse migratory contexts.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13539429/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148600248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pathway to Independence - an interview with Maëlle Bellec. 通往独立之路-采访Maëlle Bellec。

IF 4 2区生物学

Development Pub Date : 2026-09-01 Epub Date: 2026-09-09 DOI: 10.1242/dev.206032

引用次数: 0

Tailless terminates the neural stem cell temporal cascade in both the optic lobe and central brain. 无尾（TLX）终止了视叶和中央脑的神经干细胞时间级联。

IF 4 2区生物学

Development Pub Date : 2026-09-01 Epub Date: 2026-09-11 DOI: 10.1242/dev.205703

Jocelyn L Y Tang, Alex P A Donovan, Andrea H Brand

{"title":"Tailless terminates the neural stem cell temporal cascade in both the optic lobe and central brain.","authors":"Jocelyn L Y Tang, Alex P A Donovan, Andrea H Brand","doi":"10.1242/dev.205703","DOIUrl":"10.1242/dev.205703","url":null,"abstract":"Temporal patterning is an evolutionarily conserved mechanism to produce neuronal and glial diversity from common cells of origin during neurodevelopment. This process is controlled by a series of temporal transcription factors that are transiently expressed and drive the sequential production of specific progeny subtypes. Intermediate neural progenitors (INPs) and optic lobe neural stem cells (OL NSCs) share striking similarities in temporal factor expression despite divergent cells of origin. Tailless (Tll) is a terminal temporal factor in the visual system in OL NSCs. Its expression coincides with the termination of neurogenesis and onset of gliogenesis. Here, we report that Tll also acts as a terminal factor in Drosophila INPs, demonstrating functional conservation. Tll expression is activated by the preceding temporal factor Scarecrow, and represses odd-paired and hamlet. tll also plays a partial role in promoting gliogenesis in gliogenic NSCs. We performed genome-wide binding analysis of Tll in the OL NSCs and INPs by Targeted DamID, revealing both conserved and divergent targets, reflecting differences in regulatory outcomes. We show that temporal patterning mechanisms are conserved between different brain regions, whilst facilitating lineage-specific outputs.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13615674/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148817883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulatory architecture controlling terminal differentiation of an interoceptive paraneuron in Caenorhabditis elegans. 秀丽隐杆线虫内感受副神经元终末分化的调控结构。

IF 4 2区生物学

Development Pub Date : 2026-09-01 Epub Date: 2026-08-28 DOI: 10.1242/dev.205749

Hongzhu Ji, Berta Vidal, Elisabeth Conklin, Tergel Enkhtuvshin, Nathan E Schroeder, Oliver Hobert

{"title":"Regulatory architecture controlling terminal differentiation of an interoceptive paraneuron in Caenorhabditis elegans.","authors":"Hongzhu Ji, Berta Vidal, Elisabeth Conklin, Tergel Enkhtuvshin, Nathan E Schroeder, Oliver Hobert","doi":"10.1242/dev.205749","DOIUrl":"10.1242/dev.205749","url":null,"abstract":"Interoceptive paraneurons are neuron-like cells located within internal epithelial cell surfaces that sense internal stimuli to evoke specific behavioral or physiological responses. The elucidation of terminal differentiation programs of paraneurons is expected to provide insights into how epithelial cells acquire neuron-like features during development and possibly also over evolutionary time. We define here the transcriptional programs that control the terminal differentiation of an interoceptive paraneuron class in the nematode Caenorhabditis elegans, called uv1. We show that, as in canonical neurons, the neuron-like secretory features of uv1 are controlled by a combination of CUT homeobox genes, while the combinatorial terminal gene battery that defines the unique functional features of uv1 is jointly controlled by a combination of at least three transcription factors: a LIM homeodomain (LIN-11), a SoxD (EGL-13) and a Pax (EGL-38) family protein. These factors act in a terminal selector-type manner to jointly co-regulate the many distinct uv1-paraneuron specific molecular features, such as sensory and neuromodulatory receptors, neuropeptides, and tyramine synthesis machinery. Our findings demonstrate notable similarities in the dichotomous architecture of gene regulatory programs of neurons and paraneurons.","PeriodicalId":11375,"journal":{"name":"Development","volume":"153 17","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13615684/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148839561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An avidity-driven mechanism of extracellular BMP regulation by Twisted gastrulation. 扭曲原肠胚形成调控细胞外BMP的亲切度驱动机制。

IF 4 2区生物学

Development Pub Date : 2026-08-15 Epub Date: 2026-02-16 DOI: 10.1242/dev.204584

Gareth Moore, Raluca Revici, Lauren Forbes Beadle, Catherine Sutcliffe, Holly Birchenough, Clair Baldock, Hilary L Ashe

{"title":"An avidity-driven mechanism of extracellular BMP regulation by Twisted gastrulation.","authors":"Gareth Moore, Raluca Revici, Lauren Forbes Beadle, Catherine Sutcliffe, Holly Birchenough, Clair Baldock, Hilary L Ashe","doi":"10.1242/dev.204584","DOIUrl":"10.1242/dev.204584","url":null,"abstract":"During dorsoventral patterning of bilaterian embryos, the conserved regulator Twisted gastrulation (Tsg) modulates BMP signalling by binding Chordin/Short gastrulation (Sog). Here, we elucidate the mechanism by which Tsg interacts with Sog/Chordin to promote formation of the inhibitory Tsg-Sog/Chordin-BMP complex and regulate BMP signalling extracellularly. We identify and validate in vitro a hydrophobic interface in the Tsg C-terminal domain that binds Chordin. Mutation of this epitope in Drosophila Tsg (TsgL100A) results in an unexpectedly mild perturbation to embryonic BMP gradient formation. We show that a protostome-specific Tsg C-terminal extension also binds Sog, and the presence of this second binding site allows partial rescue of Sog interaction with TsgL100A in the presence of BMP. Consistent with this, a truncated Tsg protein lacking both Sog binding regions is unable to support BMP gradient formation in vivo. As our data show that disruption of either Sog binding site in Tsg, but not both, can be overcome by Tsg-BMP and Sog-BMP interactions, we present a new avidity-driven mechanism of BMP gradient formation that will be relevant to a broad range of developmental contexts.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145917236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Harnessing the murine inner cell mass mechanical environment enhances derivation of in vitro nascent primitive endoderm precursor cells. 利用小鼠内细胞群力学环境促进体外新生原始内胚层前体细胞的分化。

IF 4 2区生物学

Development Pub Date : 2026-08-15 Epub Date: 2026-06-10 DOI: 10.1242/dev.205226

Elena Corujo-Simon, Siiri I Salomaa, Lawrence E Bates, Ayaka Yanagida, William Mansfield, Hannes Becher, Jennifer Nichols, Kevin J Chalut

{"title":"Harnessing the murine inner cell mass mechanical environment enhances derivation of in vitro nascent primitive endoderm precursor cells.","authors":"Elena Corujo-Simon, Siiri I Salomaa, Lawrence E Bates, Ayaka Yanagida, William Mansfield, Hannes Becher, Jennifer Nichols, Kevin J Chalut","doi":"10.1242/dev.205226","DOIUrl":"10.1242/dev.205226","url":null,"abstract":"Stem cell-based models resembling murine blastocysts represent a useful system to investigate subsequent developmental processes. While existing cell lines derived from epiblast and trophectoderm can be aggregated to form 'blastoids', some previously tested in vitro cultured extra-embryonic endoderm cells tended to progress to later stages of development, so integrated inefficiently into blastoids. We attempted to capture the precursor population for extra-embryonic endoderm in vitro by reproducing the mechanical environment of the in vivo peri-implantation embryo as closely as possible. We investigated expression of candidate cell adhesion receptor integrins in the blastocyst inner cell mass, and from this information we assembled an extracellular matrix intended to support primitive endoderm growth by promoting signalling pathways responsible for specification of this lineage. In addition, inner cell mass cells from blastocysts were plated on soft or stiff substrates to investigate whether an appropriate mechano-environment could enhance their self-renewal as primitive endoderm in culture. We could expand nascent primitive endoderm cell lines over several passages, which provided a reproducible, albeit short-term, system sufficient to identify some essential requirements for early primitive endoderm expansion and function.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13286370/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148204620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A developmental biologist's guide to mechanics. 发育生物学家的力学指南。

IF 4 2区生物学

Development Pub Date : 2026-08-15 Epub Date: 2026-08-26 DOI: 10.1242/dev.205785

Jialing Cao, Alessandro Chiappori, Sylvain Landiech, Raphaël Clément, Pierre-François Lenne

{"title":"A developmental biologist's guide to mechanics.","authors":"Jialing Cao, Alessandro Chiappori, Sylvain Landiech, Raphaël Clément, Pierre-François Lenne","doi":"10.1242/dev.205785","DOIUrl":"https://doi.org/10.1242/dev.205785","url":null,"abstract":"Developmental biology seeks to explain how living systems self-organize into functional, robust forms. While molecular and genetic approaches have revealed how biological information is encoded and processed, they often do not explain how this information is translated into physical shape. Here, we introduce the mechanical principles underlying tissue morphogenesis, aimed at developmental biologists with little background in physics. This Primer is structured around three core pillars. First, force generation describes how active and passive processes within cells and tissues drive deformation and motion. Second, constitutive behavior defines how living matter responds to these forces, spanning elastic, viscous and plastic regimes. Third, geometry and boundary constraints set the spatial constraints that shape mechanical interactions at the tissue and organismal levels. Together, these elements provide a framework for understanding how form emerges from the interplay between forces, material properties and geometry. We illustrate key examples across developmental processes and highlight recent advances and future challenges, integrating mechanical and molecular perspectives to guide the study of morphogenesis.","PeriodicalId":11375,"journal":{"name":"Development","volume":"153 16","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148817881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0