Development最新文献_第8页

NR4A1 and NR4A2 orphan nuclear receptors regulate endothelial-to-hematopoietic transition in mouse hematopoietic stem cell specification. NR4A1和NR4A2孤儿核受体在小鼠造血干细胞规格化过程中调控内皮细胞向造血干细胞的转变。

IF 3.7 2区生物学

Development Pub Date : 2024-11-15 Epub Date: 2024-11-26 DOI: 10.1242/dev.201957

Diana Sá da Bandeira, Chris D Nevitt, Felipe Segato Dezem, Maycon Marção, Yutian Liu, Zakiya Kelley, Hannah DuBose, Ashley Chabot, Trent Hall, Claire Caprio, Victoria Okhomina, Guolian Kang, Jasmine Plummer, Shannon McKinney-Freeman, Wilson K Clements, Miguel Ganuza

{"title":"NR4A1 and NR4A2 orphan nuclear receptors regulate endothelial-to-hematopoietic transition in mouse hematopoietic stem cell specification.","authors":"Diana Sá da Bandeira, Chris D Nevitt, Felipe Segato Dezem, Maycon Marção, Yutian Liu, Zakiya Kelley, Hannah DuBose, Ashley Chabot, Trent Hall, Claire Caprio, Victoria Okhomina, Guolian Kang, Jasmine Plummer, Shannon McKinney-Freeman, Wilson K Clements, Miguel Ganuza","doi":"10.1242/dev.201957","DOIUrl":"10.1242/dev.201957","url":null,"abstract":"Hematopoietic stem cells (HSCs) sustain life-long hematopoiesis and emerge during mid-gestation from hemogenic endothelial progenitors via an endothelial-to-hematopoietic transition (EHT). The full scope of molecular mechanisms governing this process remains unclear. The NR4A subfamily of orphan nuclear receptors act as tumor suppressors in myeloid leukemogenesis and have never been implicated in HSC specification. Here, we report that Nr4a1 and Nr4a2 expression is upregulated in hemogenic endothelium during EHT. Progressive genetic ablation of Nr4a gene dosage results in a gradual decrease in numbers of nascent c-Kit+ hematopoietic progenitors in developing embryos, c-Kit+ cell cluster size in the dorsal aorta, and a block in HSC maturation, revealed by an accumulation of pro-HSCs and pre-HSC-type I cells and decreased numbers of pre-HSC-type II cells. Consistent with these observations, cells isolated from embryonic day 11.5 Nr4a1-/-; Nr4a2-/- aorta-gonads-mesonephros are devoid of in vivo long-term hematopoietic repopulating potential. Molecularly, employing spatial transcriptomic analysis we determined that the genetic ablation of Nr4a1 and Nr4a2 prevents Notch signaling from being downregulated in intra-aortic clusters and thus for pro-HSCs to mature into HSCs. Interestingly, this defect is partially rescued by ex vivo culture of dissected aorta-gonads-mesonephros with SCF, IL3 and FLT3L, which may bypass Notch-dependent regulation. Overall, our data reveal a role for the NR4A family of orphan nuclear receptors in EHT.","PeriodicalId":11375,"journal":{"name":"Development","volume":"151 22","pages":""},"PeriodicalIF":3.7,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11634030/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142715599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The people behind the papers - Kerim Anlaş and Vikas Trivedi. 报纸背后的人物--克里姆-安拉什（Kerim Anlaş）和维卡斯-特里维迪（Vikas Trivedi）。

IF 3.7 2区生物学

Development Pub Date : 2024-11-15 Epub Date: 2024-11-18 DOI: 10.1242/dev.204481

引用次数: 0

Dynamic protein assembly and architecture of the large solitary membraneless organelle during germline development in the wasp Nasonia vitripennis. 黄蜂 Nasonia vitripennis 生殖发育过程中大型孤膜无细胞器的动态蛋白质组装和结构。

IF 3.7 2区生物学

Development Pub Date : 2024-11-15 Epub Date: 2024-11-19 DOI: 10.1242/dev.202877

Kabita Kharel, Samuel J Tindell, Allie Kemph, Ryan Schmidtke, Emma Alexander, Jeremy A Lynch, Alexey L Arkov

{"title":"Dynamic protein assembly and architecture of the large solitary membraneless organelle during germline development in the wasp Nasonia vitripennis.","authors":"Kabita Kharel, Samuel J Tindell, Allie Kemph, Ryan Schmidtke, Emma Alexander, Jeremy A Lynch, Alexey L Arkov","doi":"10.1242/dev.202877","DOIUrl":"10.1242/dev.202877","url":null,"abstract":"Throughout metazoa, germ cells assemble RNA-protein organelles (germ granules). In Drosophila ovaries, perinuclear nuage forms in the nurse cells, while compositionally similar polar granules form in the oocyte. A similar system appears to exist in the distantly related (∼350 million years) wasp Nasonia, with some surprising divergences. Nuage is similarly formed in Nasonia, except that anterior nurse cells accumulate significantly more nuage, in association with high levels of DNA double-strand breaks, suggesting that increased transposon activity anteriorly is silenced by high nuage levels. In the oocyte, the germ plasm forms a single granule that is 40 times larger than a homologous Drosophila polar granule. While conserved germ granule proteins are recruited to the oosome, they show unusual localization: Tudor protein forms a shell encapsulating the embryonic oosome, while small Oskar/Vasa/Aubergine granules coalesce interiorly. Wasp Vasa itself is unusual since it has an alternative splice form that includes a previously unreported nucleoporin-like phenylalanine-glycine repeat domain. Our work is consistent with the high degree of evolutionary plasticity of membraneless organelles, and describes a new experimental model and resources for studying biomolecular condensates.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11607683/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142497088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A genome-engineered tool set for Drosophila TGF-β/BMP signaling studies. 用于果蝇 TGF-β/BMP 信号研究的基因组工程工具集。

IF 3.7 2区生物学

Development Pub Date : 2024-11-15 Epub Date: 2024-11-18 DOI: 10.1242/dev.204222

Clara-Maria Ell, Abu Safyan, Mrinal Chayengia, Manuela M M Kustermann, Jennifer Lorenz, Melanie Schächtle, George Pyrowolakis

{"title":"A genome-engineered tool set for Drosophila TGF-β/BMP signaling studies.","authors":"Clara-Maria Ell, Abu Safyan, Mrinal Chayengia, Manuela M M Kustermann, Jennifer Lorenz, Melanie Schächtle, George Pyrowolakis","doi":"10.1242/dev.204222","DOIUrl":"10.1242/dev.204222","url":null,"abstract":"Ligands of the TGF-β/BMP superfamily are crucially involved in the regulation of growth, patterning and organogenesis and can act as long-range morphogens. Essential for understanding TGF-β/BMP signaling dynamics and regulation are tools that allow monitoring and manipulating pathway components at physiological expression levels and endogenous spatiotemporal patterns. We used genome engineering to generate a comprehensive library of endogenously epitope- or fluorescent-tagged versions of receptors, co-receptors, transcription factors and key feedback regulators of the Drosophila BMP and Activin signaling pathways. We demonstrate that the generated alleles are biologically active and can be used for assessing tissue and subcellular distribution of the corresponding proteins. Furthermore, we show that the genomic platforms can be used for in locus structure-function and cis-regulatory analyses. Finally, we present a complementary set of protein binder-based tools, which allow visualization as well as manipulation of the stability and subcellular localization of epitope-tagged proteins, providing new tools for the analysis of BMP signaling and beyond.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11607693/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142567939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SMPD4-mediated sphingolipid metabolism regulates brain and primary cilia development. SMPD4介导的鞘脂代谢调节大脑和初级纤毛的发育。

IF 3.7 2区生物学

Development Pub Date : 2024-11-15 DOI: 10.1242/dev.202645

Katherine A Inskeep, Bryan Crase, Thamara Dayarathna, Rolf W Stottmann

引用次数: 0

The protamines of the spider Steatoda sp. provide an example of liquid-liquid phase separation chromatin transitions during spermiogenesis. 蜘蛛 Steatoda sp.的原胺为精子发生过程中的液-液相分离染色质转换提供了一个实例。

IF 3.7 2区生物学

Development Pub Date : 2024-11-15 Epub Date: 2024-11-18 DOI: 10.1242/dev.203134

Melissa R Leyden, Peter Michalik, Luciana Baruffaldi, Susheen Mahmood, Ladan Kalani, Donald F Hunt, Jose Maria Eirin-Lopez, Maydianne C B Andrade, Jeffrey Shabanowitz, Juan Ausió

{"title":"The protamines of the spider Steatoda sp. provide an example of liquid-liquid phase separation chromatin transitions during spermiogenesis.","authors":"Melissa R Leyden, Peter Michalik, Luciana Baruffaldi, Susheen Mahmood, Ladan Kalani, Donald F Hunt, Jose Maria Eirin-Lopez, Maydianne C B Andrade, Jeffrey Shabanowitz, Juan Ausió","doi":"10.1242/dev.203134","DOIUrl":"10.1242/dev.203134","url":null,"abstract":"Although there is extensive information about sperm nuclear basic proteins (SNBP) in vertebrates, there is, by comparison, very little information in Arthropoda. This study aims to contribute to filling this gap by analyzing these proteins in the sperm of the noble false widow spider Steatoda nobilis (order Araneae, family Theridiidae). To this end, we have developed a protein extraction method that allows the extraction of both cysteine-containing and non-cysteine-containing protamines that is suitable for the preparation and analysis of SNBPs from samples in which the amount of starting tissue material is limited. We carried out top-down mass spectrometry sequencing and molecular phylogenetic analyses to characterize the protamines of S. nobilis and other spiders. We also used electron microscopy to analyze the chromatin organization of the Steatoda sperm and we found it to exhibit liquid-liquid phase spinodal decomposition during the late stages of spermiogenesis. These experiments further our knowledge on the distribution of SNBPs within the animal kingdom and provide additional support for a proposed evolutionary origin of many protamines from a histone H1 (H5) replication-independent precursor.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11607694/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142497090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Early autonomous patterning of the anteroposterior axis in gastruloids. 胃体前后轴的早期自主模式化

IF 3.7 2区生物学

Development Pub Date : 2024-11-15 Epub Date: 2024-11-18 DOI: 10.1242/dev.202171

Kerim Anlaş, Nicola Gritti, Fumio Nakaki, Laura Salamó Palau, Sham Leilah Tlili, David Oriola, Krisztina Arató, Jia Le Lim, James Sharpe, Vikas Trivedi

{"title":"Early autonomous patterning of the anteroposterior axis in gastruloids.","authors":"Kerim Anlaş, Nicola Gritti, Fumio Nakaki, Laura Salamó Palau, Sham Leilah Tlili, David Oriola, Krisztina Arató, Jia Le Lim, James Sharpe, Vikas Trivedi","doi":"10.1242/dev.202171","DOIUrl":"10.1242/dev.202171","url":null,"abstract":"Minimal in vitro systems composed of embryonic stem cells (ESCs) have been shown to recapitulate the establishment of the anteroposterior (AP) axis. In contrast to the native embryo, ESC aggregates - such as gastruloids - can break symmetry, which is demarcated by polarization of the mesodermal marker T, autonomously without any localized external cues. However, associated earliest patterning events, such as the spatial restriction of cell fates and concomitant transcriptional changes, remain poorly understood. Here, we dissect the dynamics of AP axis establishment in mouse gastruloids, particularly before external Wnt stimulation. Through single-cell RNA sequencing, we identify key cell state transitions and the molecular signatures of T+ and T- populations underpinning AP polarization. We also show that this process is robust to modifications of aggregate size. Finally, transcriptomic comparison with the mouse embryo indicates that gastruloids develop similar mesendodermal cell types, despite initial differences in their primed pluripotent populations, which adopt a more mesenchymal state in lieu of an epiblast-like transcriptome. Hence, our findings suggest the possibility of alternate ESC states in vivo and in vitro that can converge onto similar cell fates.","PeriodicalId":11375,"journal":{"name":"Development","volume":"151 22","pages":""},"PeriodicalIF":3.7,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11607688/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142647661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A new cell culture resource for investigations of reptilian gene function. 研究爬行动物基因功能的新细胞培养资源。

IF 3.7 2区生物学

Development Pub Date : 2024-11-15 Epub Date: 2024-11-22 DOI: 10.1242/dev.204275

Sukhada P Samudra, Sungdae Park, Elizabeth A Esser, Tryggvi P McDonald, Arianna M Borges, Jonathan Eggenschwiler, Douglas B Menke

{"title":"A new cell culture resource for investigations of reptilian gene function.","authors":"Sukhada P Samudra, Sungdae Park, Elizabeth A Esser, Tryggvi P McDonald, Arianna M Borges, Jonathan Eggenschwiler, Douglas B Menke","doi":"10.1242/dev.204275","DOIUrl":"10.1242/dev.204275","url":null,"abstract":"The establishment of CRISPR/Cas9 gene editing in Anolis sagrei has positioned this species as a powerful model for studies of reptilian gene function. To enhance this model, we developed an immortalized lizard fibroblast cell line (ASEC-1) for the exploration of reptilian gene function in cellular processes. We demonstrate the use of this cell line by scrutinizing the role of primary cilia in lizard Hedgehog (Hh) signaling. Using CRISPR/Cas9 mutagenesis, we disrupted the ift88 gene, which is required for ciliogenesis in diverse organisms. We determined that loss of itf88 from lizard cells leads to an absence of primary cilia, a partial derepression of gli1 transcription, and an inability of the cells to respond to the Smoothened agonist, SAG. Through a cross-species analysis of SAG-induced transcriptional responses in cultured limb bud cells, we further determined that ∼46% of genes induced as a response to Hh pathway activation in A. sagrei are also SAG responsive in Mus musculus limb bud cells. Our results highlight conserved and diverged aspects of Hh signaling in anoles and establish a new resource for investigations of reptilian gene function.","PeriodicalId":11375,"journal":{"name":"Development","volume":"151 22","pages":""},"PeriodicalIF":3.7,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11607698/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142686174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment of CRISPR/Cas9-based knock-in in a hemimetabolous insect: targeted gene tagging in the cricket Gryllus bimaculatus. 在半代谢昆虫中建立基于 CRISPR/Cas9 的基因敲入技术：蟋蟀 Gryllus bimaculatus 的定向基因标记。

IF 3.7 2区生物学

Development Pub Date : 2024-11-08 DOI: 10.1242/dev.199746

Yuji Matsuoka, Taro Nakamura, Takahito Watanabe, Austen A Barnett, Sayuri Tomonari, Guillem Ylla, Carrie A Whittle, Sumihare Noji, Taro Mito, Cassandra G Extavour

{"title":"Establishment of CRISPR/Cas9-based knock-in in a hemimetabolous insect: targeted gene tagging in the cricket Gryllus bimaculatus.","authors":"Yuji Matsuoka, Taro Nakamura, Takahito Watanabe, Austen A Barnett, Sayuri Tomonari, Guillem Ylla, Carrie A Whittle, Sumihare Noji, Taro Mito, Cassandra G Extavour","doi":"10.1242/dev.199746","DOIUrl":"https://doi.org/10.1242/dev.199746","url":null,"abstract":"Studies of traditional model organisms like the fruit fly Drosophila melanogaster have contributed immensely to our understanding of the genetic basis of developmental processes. However, the generalizability of these findings cannot be confirmed without functional genetic analyses in additional organisms. Direct genome editing using targeted nucleases has the potential to transform hitherto poorly-understood organisms into viable laboratory organisms for functional genetic study. To this end, here we present a method to induce targeted genome knock-out and knock-in of desired sequences in an insect that serves as an informative contrast to Drosophila, the cricket Gryllus bimaculatus. The efficiency of germ line transmission of induced mutations is comparable to that reported for other well-studied laboratory organisms, and knock-ins targeting introns yield viable, fertile animals in which knock-in events are directly detectable by visualization of a fluorescent marker in the expression pattern of the targeted gene. Combined with the recently assembled and annotated genome of this cricket, this knock-in/knock-out method increases the viability of G. bimaculatus as a tractable system for functional genetics in a basally branching insect.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142603764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The GTPase RAB6 is required for stem cell maintenance and cell migration in the gut epithelium. 肠道上皮细胞的干细胞维持和细胞迁移需要 GTPase RAB6。

IF 3.7 2区生物学

Development Pub Date : 2024-11-01 Epub Date: 2024-10-21 DOI: 10.1242/dev.203038

Pierre Simonin, Gehenna Lobo Guerrero, Sabine Bardin, Ram Venkata Gannavarapu, Denis Krndija, Joseph Boyd, Stephanie Miserey, Danijela Matic Vignjevic, Bruno Goud

{"title":"The GTPase RAB6 is required for stem cell maintenance and cell migration in the gut epithelium.","authors":"Pierre Simonin, Gehenna Lobo Guerrero, Sabine Bardin, Ram Venkata Gannavarapu, Denis Krndija, Joseph Boyd, Stephanie Miserey, Danijela Matic Vignjevic, Bruno Goud","doi":"10.1242/dev.203038","DOIUrl":"10.1242/dev.203038","url":null,"abstract":"Intestinal epithelial cells, which are instrumental in nutrient absorption, fluid regulation, and pathogen defense, undergo continuous proliferation and differentiation within the intestinal crypts, migrating towards the luminal surface where they are eventually shed. RAB GTPases are key regulators of intracellular vesicular trafficking and are involved in various cellular processes, including cell migration and polarity. Here, we investigated the role of RAB6 in the development and maintenance of the gut epithelium. We generated conditional knockout mice with RAB6 specifically deleted in the gut epithelium. We found that deletion of the Rab6a gene resulted in embryonic lethality. In adult mice, RAB6 depletion led to altered villus architecture and impaired junction integrity without affecting the segregation of apical and basolateral membrane domains. Further, RAB6 depletion slowed down cell migration and adversely affected both cell proliferation and stem cell maintenance. Notably, the absence of RAB6 resulted in a diminished number of functional stem cells, as evidenced by the rapid death of isolated crypts from Rab6a KO mice when cultured as 3D organoids. Together, these results underscore the essential role of RAB6 in maintaining gut epithelial homeostasis.","PeriodicalId":11375,"journal":{"name":"Development","volume":"151 21","pages":""},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11529276/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142460573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0