Development最新文献_第8页

In preprints: setting the tempo of development. 在预印本中：设定发展的速度。

IF 3.7 2区生物学

Development Pub Date : 2025-07-15 Epub Date: 2025-07-18 DOI: 10.1242/dev.205069

Gabriel E Valdebenito, Margarete Diaz-Cuadros

引用次数: 0

Deciphering the role of cis-regulatory elements and TFAP2C in the activation of zygotic Sox2 expression in mouse preimplantation embryos. 解析顺式调控元件和TFAP2C在小鼠着床前胚胎合子Sox2表达激活中的作用。

IF 3.6 2区生物学

Development Pub Date : 2025-07-15 Epub Date: 2025-07-18 DOI: 10.1242/dev.204626

Jaehwan Kim, Chad S Driscoll, Lijia Li, Catherine A Wilson, Wei Xie, Jason G Knott

{"title":"Deciphering the role of cis-regulatory elements and TFAP2C in the activation of zygotic Sox2 expression in mouse preimplantation embryos.","authors":"Jaehwan Kim, Chad S Driscoll, Lijia Li, Catherine A Wilson, Wei Xie, Jason G Knott","doi":"10.1242/dev.204626","DOIUrl":"10.1242/dev.204626","url":null,"abstract":"Cell fate decisions in preimplantation embryos require the coordinated expression of pluripotency and lineage-specific transcription factors. SOX2 represents the first pluripotency regulator for which expression is restricted to the inside cells of mouse preimplantation embryos. However, the genetic mechanisms that activate the expression of zygotic Sox2 are poorly understood. Here, we report that Sox2 expression in mouse embryos is controlled by the actions of key cis-regulatory elements, including a proximal promoter and super enhancer. We show that TFAP2C, a key trophoblast lineage regulator, binds to the Sox2 proximal promoter to activate its expression. Lastly, we provide evidence that TFAP2C and the HIPPO signaling pathway cooperatively regulate Sox2 expression. In summary, this work has important implications for understanding how conventional trophoblast transcription factors, such as TFAP2C, contribute to the activation of early pluripotency genes to facilitate divergent cellular states that support lineage formation.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12338974/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144539474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

H3K36me3 modification by SETD2 is essential for Col11a2 and Sema3e transcription to maintain dentinogenesis in mice. SETD2修饰H3K36me3是Col11a2和Sema3e转录维持小鼠牙本质形成的必要条件。

IF 3.6 2区生物学

Development Pub Date : 2025-07-15 Epub Date: 2025-07-14 DOI: 10.1242/dev.204352

Jiaxin Niu, Jing Fu, Hao Feng, Jiahao Han, Zhi Chen, Guobin Yang, Guohua Yuan

{"title":"H3K36me3 modification by SETD2 is essential for Col11a2 and Sema3e transcription to maintain dentinogenesis in mice.","authors":"Jiaxin Niu, Jing Fu, Hao Feng, Jiahao Han, Zhi Chen, Guobin Yang, Guohua Yuan","doi":"10.1242/dev.204352","DOIUrl":"10.1242/dev.204352","url":null,"abstract":"Dentin is a major mineralized component of teeth generated by odontoblasts. Several types of histone methylation have been reported to play important roles in odontoblast differentiation and dentinogenesis. However, the role of methylation on histone 3 at lysine 36 (H3K36) remains enigmatic. Here, we demonstrate high expression of SETD2, a methyltransferase catalyzing the trimethylation of H3K36 (H3K36me3), in the odontoblast layer. In vitro knockdown experiments and in vivo observations of two conditional knockout mouse models reveal that SETD2 is essential for odontoblast differentiation and dentinogenesis. Integrated analyses of RNA sequencing and spike-in CUT&Tag sequencing data show that SETD2 is crucial for both H3K36me3 occupancy at the loci of Col11a2 and Sema3e and their transcription. Further experiments verify that COL11A2 and SEMA3E act upstream of AKT1 signaling, promoting odontoblastic differentiation. In vitro and in vivo activation of AKT1 using SC79 (an AKT activator) partially rescues the impaired odontoblast differentiation caused by Setd2 knockdown or deficiency. Therefore, our findings indicate that H3K36me3 mediated by SETD2 is essential for dentinogenesis by regulating the expression of Col11a2 and Sema3e and AKT1 signaling.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12338916/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144483598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evolution of a novel left-right asymmetry in organ size by co-option of a tissue rotation process. 通过组织旋转过程的共同选择，一种新的器官大小左右不对称的进化。

IF 3.6 2区生物学

Development Pub Date : 2025-07-15 Epub Date: 2025-07-31 DOI: 10.1242/dev.204662

Bénédicte M Lefèvre, Marine Delvigne, Aurélie Camprodon, Josué Vidal, Virginie Courtier-Orgogozo, Michael Lang

{"title":"Evolution of a novel left-right asymmetry in organ size by co-option of a tissue rotation process.","authors":"Bénédicte M Lefèvre, Marine Delvigne, Aurélie Camprodon, Josué Vidal, Virginie Courtier-Orgogozo, Michael Lang","doi":"10.1242/dev.204662","DOIUrl":"10.1242/dev.204662","url":null,"abstract":"Left-right asymmetries have repeatedly evolved in diverse animals and affect the position, shape or size of specific organs. How novel left-right asymmetries arise remains unknown. Here, we examined Drosophila pachea, where males have evolved unique asymmetric genitalia lobes and a right-sided copulation posture in the past 3-6 million years. We found that male asymmetric lobes grow in pupae during a 360° clockwise genitalia rotation, which is a conserved and widespread developmental process in flies. Using two complementary approaches, drug application and a CRISPR-induced myo1D mutant, we altered genitalia rotation and found that asymmetric lobe sizes depend on genitalia rotation completion, while the sidedness of lobe asymmetry is determined by the rotation direction. We then investigated the impact of genital asymmetry on copulation posture. Males with reversed genital asymmetry still mate in the typical right-sided posture, indicating that right-sided behavior is not determined by asymmetric male genitalia. Our study reveals that a novel genitalia asymmetry has evolved through the co-option of a pre-existing tissue remodeling process. Tissue rotation represents a previously unreported mechanism, through which a bilateral organ can acquire a left-right size asymmetry.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144539475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The people behind the papers - Bénédicte Marie Lefèvre and Michael Lang. 这些文件背后的人——bsamnsamade Marie lef<e:1>和Michael Lang。

IF 3.6 2区生物学

Development Pub Date : 2025-07-15 Epub Date: 2025-07-31 DOI: 10.1242/dev.205090

引用次数: 0

Correction: Spatial cell fate manipulation of human pluripotent stem cells by controlling the microenvironment using photocurable hydrogel. 校正：利用光固化水凝胶控制微环境来操纵人类多能干细胞的空间细胞命运。

IF 3.6 2区生物学

Development Pub Date : 2025-07-15 Epub Date: 2025-07-24 DOI: 10.1242/dev.205058

Zhe Wang, Akira Numada, Fumi Wagai, Yusuke Oda, Masatoshi Ohgushi, Koichiro Maki, Taiji Adachi, Mototsugu Eiraku

引用次数: 0

Caenorhabditis elegans LET-381 and DMD-4 control development of the mesodermal HMC endothelial cell. 秀丽隐杆线虫LET-381和DMD-4控制中胚层HMC内皮细胞的发育。

IF 3.6 2区生物学

Development Pub Date : 2025-07-15 Epub Date: 2025-07-29 DOI: 10.1242/dev.204622

Nikolaos Stefanakis, Jasmine Xi, Jessica Jiang, Shai Shaham

{"title":"Caenorhabditis elegans LET-381 and DMD-4 control development of the mesodermal HMC endothelial cell.","authors":"Nikolaos Stefanakis, Jasmine Xi, Jessica Jiang, Shai Shaham","doi":"10.1242/dev.204622","DOIUrl":"10.1242/dev.204622","url":null,"abstract":"Endothelial cells form the inner layer of blood vessels and play key roles in circulatory system development and function. A variety of endothelial cell types have been described through gene expression and transcriptome studies; nonetheless, the transcriptional programs that specify endothelial cell fate and maintenance are not well understood. To uncover such regulatory programs, we studied the C. elegans head mesodermal cell (HMC), a non-contractile mesodermal cell bearing molecular and functional similarities to vertebrate endothelial cells. Here, we demonstrate that a Forkhead transcription factor, LET-381, is required for HMC fate specification and maintenance of HMC gene expression. DMD-4, a DMRT transcription factor, acts downstream of and in conjunction with LET-381 to mediate these functions. Independently of LET-381, DMD-4 also represses the expression of genes associated with a different, non-HMC, mesodermal fate. Our studies uncover essential roles for FoxF transcriptional regulators in endothelial cell development and suggest that FoxF co-functioning target transcription factors promote specific non-contractile mesodermal fates.","PeriodicalId":11375,"journal":{"name":"Development","volume":"152 14","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12377808/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144728753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of klf2 in innovation of the Pmar1-HesC double-negative gate in echinoderms. 冗余抑制驱动的监管创新；klf2在棘皮动物Pmar1-HesC双负通道创新中的作用。

IF 3.7 2区生物学

Development Pub Date : 2025-07-15 Epub Date: 2025-07-21 DOI: 10.1242/dev.204492

Nina Levin, Natalia Gogoleva, Atsuko Yamazaki, Shumpei Yamakawa, Yoshiaki Morino, Hiroshi Wada

引用次数: 0

Fbxw7 regulates N1ICD and Sox9 perdurance during pancreas development to ensure proper cell lineage allocation and segregation. Fbxw7调节胰腺发育过程中的N1ICD和Sox9耐久性，以确保适当的细胞谱系分配和分离。

IF 3.6 2区生物学

Development Pub Date : 2025-07-15 Epub Date: 2025-07-28 DOI: 10.1242/dev.204596

Diana Wichmann Graff, Emilie Liv Bang-Jensen, Anuska la Rosa Egeskov-Madsen, Palle Serup, Philip A Seymour

{"title":"Fbxw7 regulates N1ICD and Sox9 perdurance during pancreas development to ensure proper cell lineage allocation and segregation.","authors":"Diana Wichmann Graff, Emilie Liv Bang-Jensen, Anuska la Rosa Egeskov-Madsen, Palle Serup, Philip A Seymour","doi":"10.1242/dev.204596","DOIUrl":"10.1242/dev.204596","url":null,"abstract":"In the developing mouse pancreas, Notch signaling first maintains multipotent pancreatic progenitor cells (MPCs), suppressing early endocrine differentiation, then promotes their adoption of a duct/endocrine-bipotent progenitor (BP) fate at the expense of Notch-independent pro-acinar progenitors (PACs). Active Notch signaling is terminated by Notch intracellular domain (NICD) degradation via the E3 ubiquitin ligase SCFFbxw7, which also regulates the stability of other unidentified substrates in developing pancreas. We show here that endodermal deletion of the substrate recognition component Fbxw7 dose-dependently suppresses early endocrine differentiation, which is consistent with Notch1 ICD (N1ICD) upregulation in MPCs, then perturbs later acinar and ductal morphogenesis. PACs of Fbxw7 mouse mutants ectopically express Sox9 and N1ICD, which is associated with defective acinar differentiation, while their ducts are cystic with intermingled endocrine cells and inappropriate external openings. While genetic blockade of Notch transduction normalizes early endocrine differentiation in Fbxw7 mouse mutants, it is unable to rescue the later acinar phenotype, which, instead, can be normalized by Sox9 haploinsufficiency. Fbxw7 is thus required to limit N1ICD and Sox9 perdurance in the developing pancreas to enable appropriate early endocrine differentiation and to allow the complete segregation of BP and PAC lineages.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144539476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential regulation of eye specification in Drosophila by Polycomb Group epigenetic repressors. Polycomb Group （PcG）表观遗传抑制因子对果蝇眼睛特征的差异调控。

IF 3.6 2区生物学

Development Pub Date : 2025-07-15 Epub Date: 2025-07-29 DOI: 10.1242/dev.204317

Haley E Brown, Claude Jean-Guillaume, Brandon P Weasner, Justin P Kumar

引用次数: 0