Development最新文献_第7页

Regulation of lambdoid suture morphogenesis by PDGFRα-dependent chondrocyte programs. pdgfr α依赖性软骨细胞程序对兔样骨缝线形态发生的调控。

IF 4 2区生物学

Development Pub Date : 2026-09-01 Epub Date: 2026-09-03 DOI: 10.1242/dev.205550

Garrett Bartoletti, Ryan Ebright, Haoyang Liang, Josue Jaramillo, Mimi Sammarco, Xiaojiang Xu, Fenglei He

{"title":"Regulation of lambdoid suture morphogenesis by PDGFRα-dependent chondrocyte programs.","authors":"Garrett Bartoletti, Ryan Ebright, Haoyang Liang, Josue Jaramillo, Mimi Sammarco, Xiaojiang Xu, Fenglei He","doi":"10.1242/dev.205550","DOIUrl":"10.1242/dev.205550","url":null,"abstract":"Craniosynostosis is a congenital disorder characterized by premature fusion of cranial sutures. Lambdoid synostosis is a rare and severe form of the disease, but its developmental etiology remains poorly understood. Although ectopic cartilage has been associated with lambdoid suture fusion, whether abnormal chondrocyte development is causative has not been directly tested. Here, we have investigated the role of platelet-derived growth factor receptor alpha (PDGFRα) signaling in chondrocyte development and lambdoid suture morphogenesis. Mesoderm-specific expression of an autoactivated Pdgfra allele (Pdgfra+/K) caused premature fusion of the lambdoid and occipitointerparietal sutures, preceded by excessive cartilage expansion during embryogenesis. Spatial transcriptomic analysis identified a PDGFRα-dependent transcriptional program characterized by increased Col2a1, Sox9 and Sfrp2 expression, indicating maintenance of chondrocytes in a proliferative progenitor state. Consistent with this, PDGFRα-activated chondrocytes exhibited increased proliferation. Moreover, expression of Pdgfra+/K in the Col2a1Cre lineage selectively induced occipitointerparietal suture fusion. Together, these findings demonstrate that PDGFRα signaling promotes proliferative cartilage and impairs chondrocyte maturation, identifying dysregulated chondrogenesis as a developmental mechanism underlying lambdoid craniosynostosis.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148711481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The people behind the papers - Rashi Agarwal, Joergen Benjaminsen and Joachim Wittbrodt. 这些报纸背后的人——拉什·阿加瓦尔，约根·本杰明森和约阿希姆·维特布罗德。

IF 4 2区生物学

Development Pub Date : 2026-09-01 Epub Date: 2026-09-10 DOI: 10.1242/dev.206048

引用次数: 0

In preprints - compression rather than loss drives duckweed miniaturisation. 在预印本中，压缩而不是损耗驱动浮萍小型化。

IF 4 2区生物学

Development Pub Date : 2026-09-01 Epub Date: 2026-08-25 DOI: 10.1242/dev.205947

Dylan Jones

引用次数: 0

Pathway to Independence - an interview with Yamini Ravichandran. 独立之路——对雅米尼·拉维昌兰的采访。

IF 4 2区生物学

Development Pub Date : 2026-09-01 Epub Date: 2026-08-20 DOI: 10.1242/dev.205942

引用次数: 0

Pathway to Independence - an interview with Nikhil Mishra. 独立之路-对Nikhil Mishra的采访。

IF 4 2区生物学

Development Pub Date : 2026-09-01 Epub Date: 2026-09-03 DOI: 10.1242/dev.206049

引用次数: 0

In preprints - growing thick skins: how cellular patterns emerge in the developing plant epidermis. 在预印本-生长厚皮：细胞模式如何出现在发育中的植物表皮。

IF 4 2区生物学

Development Pub Date : 2026-09-01 Epub Date: 2026-08-20 DOI: 10.1242/dev.205946

Pablo González-Suárez

引用次数: 0

Ingression of the cardiac outflow tract wall is disrupted in mice with altered β-catenin, Shh and FGF8 signaling. 在β-catenin、Shh和FGF8信号改变的小鼠中，形成下中线嵌套瓣膜肿胀的心流出道壁的浸润被破坏。

IF 4 2区生物学

Development Pub Date : 2026-09-01 Epub Date: 2026-08-27 DOI: 10.1242/dev.205695

Haiyue Feng, Yuke Ji, Zixuan Li, Jie Yao, Jing Cui, Lijun Zhang, Xiaodong Wang, Wenli Fan, Xianfa Yang, Naihe Jing, Zhongzhou Yang

{"title":"Ingression of the cardiac outflow tract wall is disrupted in mice with altered β-catenin, Shh and FGF8 signaling.","authors":"Haiyue Feng, Yuke Ji, Zixuan Li, Jie Yao, Jing Cui, Lijun Zhang, Xiaodong Wang, Wenli Fan, Xianfa Yang, Naihe Jing, Zhongzhou Yang","doi":"10.1242/dev.205695","DOIUrl":"10.1242/dev.205695","url":null,"abstract":"Previous studies from us and others suggest that the second heart field (SHF)-derived outflow tract (OFT) wall can undergo a cell fate transition to mesenchymal cells within the intercalated valve swelling (ICVS). However, the trajectory of this transition and its intrinsic regulation remain unknown. Here, we investigate cell fate determination in mouse OFT development and identify specific NKX2-5 expression in the inferior midline OFT (imOFT) wall and adjacent ICVS. scRNA-seq of E10.5 OFT revealed a transcriptional signature specific to the imICVS within the heterogeneous cell populations. We observed that cells of the imOFT wall ingress while maintaining myocyte properties, whereas the leading cells initiate a transition to a mesenchymal fate. Furthermore, these ingressing cells exhibit 'lily-bulb' collective cell migration. In addition, we found that β-catenin, Shh and FGF8 signaling are associated with imICVS formation. These results identify a previously unreported type of cell ingression and demonstrate that the imICVS appears to spatially coordinate the distribution of the OFT main cushion derived from neural crest. This study provides new insights into the role of the SHF in OFT septation and the pathogenesis of congenital heart diseases.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148618762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Loss of Twist impairs tentacle development and induces epithelial neoplasia in the sea anemone Nematostella vectensis. 失去捻度会损害海葵触手的发育并诱发海葵线虫的上皮瘤变。

IF 4 2区生物学

Development Pub Date : 2026-09-01 Epub Date: 2026-08-18 DOI: 10.1242/dev.205340

Patricio Ferrer Murguia, Julia Hagauer, Emmanuel Haillot, Aissam Ikmi, Juan D Montenegro, Alison G Cole, Ulrich Technau

{"title":"Loss of Twist impairs tentacle development and induces epithelial neoplasia in the sea anemone Nematostella vectensis.","authors":"Patricio Ferrer Murguia, Julia Hagauer, Emmanuel Haillot, Aissam Ikmi, Juan D Montenegro, Alison G Cole, Ulrich Technau","doi":"10.1242/dev.205340","DOIUrl":"10.1242/dev.205340","url":null,"abstract":"Appendages are key features of the body plans of arthropods and vertebrates, yet little is known about appendage formation outside of bilaterians. Here, we investigate the molecular regulation underlying the formation of tentacles, the appendages in cnidarians. We find that the bHLH transcription factor Twist plays a crucial role in tentacle morphogenesis and tissue homeostasis in the sea anemone Nematostella vectensis. Using CRISPR/Cas9, we show that twist mutants exhibit impaired secondary tentacle formation, delayed and reduced proliferation in budding tentacles, and the absence of microcnemes, structures that demarcate tentacle boundaries, suggesting defects in spatial patterning. Loss of Twist disrupts expression of co-expressed mesodermal transcription factors paraxis and tbx15 and perturbs the TOR-FGF signalling feedback loop necessary for normal tentacle growth. We note that the link of Twist and FGF signalling is reminiscent of limb formation in vertebrates. Moreover, starting at juveniles, mutants develop neoplasm-like epithelial overgrowth with tentacle-like molecular profiles, indicating a role for Twist in maintaining tissue homeostasis at the oral pole. Together, Twist integrates major signalling pathways to regulate secondary tentacle patterning and maintain spatial tissue organisation in the diploblastic N. vectensis.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13539428/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148535597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In preprints - the embryo, the placenta and lessons from developmental metabolism. 在预印本中——胚胎、胎盘和发育代谢的教训。

IF 4 2区生物学

Development Pub Date : 2026-09-01 Epub Date: 2026-08-26 DOI: 10.1242/dev.205945

Berna Sozen

引用次数: 0

EpiCure (Epithelial Curation): a versatile and handy tool for curation of epithelial segmentation. EpiCure（上皮细胞管理）：一个多功能和方便的工具，用于管理上皮细胞分割。

IF 4 2区生物学

Development Pub Date : 2026-09-01 Epub Date: 2026-09-03 DOI: 10.1242/dev.205701

Gaëlle Letort, Léo Valon, Arthur Michaut, Tom Cumming, Laura Xénard, Minh-Son Phan, Nicolas Dray, Curtis T Rueden, François Schweisguth, Jérôme Gros, Laure Bally-Cuif, Jean-Yves Tinevez, Romain Levayer

{"title":"EpiCure (Epithelial Curation): a versatile and handy tool for curation of epithelial segmentation.","authors":"Gaëlle Letort, Léo Valon, Arthur Michaut, Tom Cumming, Laura Xénard, Minh-Son Phan, Nicolas Dray, Curtis T Rueden, François Schweisguth, Jérôme Gros, Laure Bally-Cuif, Jean-Yves Tinevez, Romain Levayer","doi":"10.1242/dev.205701","DOIUrl":"10.1242/dev.205701","url":null,"abstract":"Despite advances in deep-learning and bioimage analysis, manual curation of segmented and tracking data is still required to extract accurate quantitative single cell temporal information from large tissue/embryo movies. However, very few tools address specifically this challenge. We present here EpiCure (Epithelial Curation), a versatile tool designed to streamline and accelerate manual curation of segmentation and tracking in 2D movies of large epithelial tissues. EpiCure uses temporal information and morphometric parameters to automatically identify segmentation and tracking errors and provides user-friendly tools to correct them. It focuses on ergonomics and offers visualization options to help navigate movies covering a large number of cells, speeding up the detection and curation of errors. EpiCure is highly interoperable, supports input from diverse segmentation tools and includes multiple export filters enabling seamless integration with downstream analysis pipelines. Using movies from several animal models, we highlight the importance of curating cell segmentation and tracking for accurate downstream analysis, and how EpiCure helps by extracting single cell dynamics and detecting cellular events in a large dataset.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148673231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0