Development最新文献_第7页

Cytokinins control secondary cell wall formation in the inflorescence stem of Arabidopsis. 细胞分裂素控制拟南芥花序茎次生细胞壁的形成。

IF 3.6 2区生物学

Development Pub Date : 2025-09-15 Epub Date: 2025-09-16 DOI: 10.1242/dev.205000

Vojtech Didi, Dominique Arnaud, Alesia Melnikava, Anna Pacinková, Radek Jupa, Radim Cegan, Jana Vasickova, Mariana Benitez, Faride Unda, Tereza Dobisova, Willi Riber, Zuzana Dostalova, Ondrej Novak, Miroslav Strnad, Roman Hobza, Shawn D Mansfield, Vít Gloser, Eva Budinska, Jan Hejatko

{"title":"Cytokinins control secondary cell wall formation in the inflorescence stem of Arabidopsis.","authors":"Vojtech Didi, Dominique Arnaud, Alesia Melnikava, Anna Pacinková, Radek Jupa, Radim Cegan, Jana Vasickova, Mariana Benitez, Faride Unda, Tereza Dobisova, Willi Riber, Zuzana Dostalova, Ondrej Novak, Miroslav Strnad, Roman Hobza, Shawn D Mansfield, Vít Gloser, Eva Budinska, Jan Hejatko","doi":"10.1242/dev.205000","DOIUrl":"https://doi.org/10.1242/dev.205000","url":null,"abstract":"Spatiotemporal control over developmental programs is vital to all organisms. Here, we show that deficiency in cytokinin signaling or biosynthesis leads to early secondary cell wall (SCW) formation in Arabidopsis inflorescence stem that associates with precocious upregulation of an SCW transcriptional cascade controlled by NAC transcription factors (NSTs/VNDs). Cytokinin signaling through AHK2/3 and the ARR1/10/12 suppresses the expression of several NST genes and SCW formation. Exogenous cytokinin application led to fast downregulation of NST1 and NST3 (NST1/3) in the wild type and reconstituted both proper development and the apical-basal gradient of NST1/3 expression in a cytokinin-deficient mutant. AHK2 and AHK3 require functional NST1 or NST3 to control SCW initiation in the interfascicular fibers, demonstrating that cytokinins act upstream of NSTs. The premature onset of a rigid SCW biosynthesis and altered expression of NSTs due to cytokinin deficiency led to the formation of smaller tracheary elements and impaired hydraulic conductivity. We conclude that cytokinins downregulate NST genes to inhibit premature SCW formation in the apical part of the inflorescence stem, facilitating thus the development of fully functional tracheary elements and interfascicular fibers.","PeriodicalId":11375,"journal":{"name":"Development","volume":"152 18","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145074585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ectodysplasin overexpression reveals spatiotemporally dynamic tooth formation competency in stickleback and zebrafish. 外胞质异常蛋白过表达揭示了棘鱼和斑马鱼牙齿形成能力的时空动态变化。

IF 3.6 2区生物学

Development Pub Date : 2025-09-15 Epub Date: 2025-09-26 DOI: 10.1242/dev.204907

Zoe Z Chen, Sujanya N Narayanan, Lena M Stagliano, Peter Q Huynh, Shivani Sundaram, Emma J Mackey, Craig T Miller, Tyler A Square

{"title":"Ectodysplasin overexpression reveals spatiotemporally dynamic tooth formation competency in stickleback and zebrafish.","authors":"Zoe Z Chen, Sujanya N Narayanan, Lena M Stagliano, Peter Q Huynh, Shivani Sundaram, Emma J Mackey, Craig T Miller, Tyler A Square","doi":"10.1242/dev.204907","DOIUrl":"10.1242/dev.204907","url":null,"abstract":"Organ initiation is often driven by extracellular signaling molecules that activate precursor cells competent to receive and respond to a given signal, yet little is known about the dynamics of competency in space and time during development. Teeth are excellent model organs for studying cellular competency because they can be activated with the addition of a single signaling ligand, Ectodysplasin (Eda). To investigate the role of Eda in tooth specification, we generated transgenic sticklebacks and zebrafish with heat shock-inducible Eda overexpression. We found that stickleback Eda can drive ectopic tooth formation in at least eight distinct morphological domains. Both zebrafish and stickleback exhibit maximal responsiveness to Eda overexpression during pioneer tooth initiation. Analysis of candidate receptor expression in sticklebacks reveals that ectopic tooth formation in the pharynx correlates with Edar and Troy expression, while only Troy expression was detected in regions of the face where teeth can form, suggesting that competency may involve spatially restricted receptor expression. These findings underscore the latent developmental potential, i.e. competency, of the vertebrate dentition and provide insights into organ competency during embryonic and post-embryonic development.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12516321/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145023096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Automated, high-throughput in situ hybridization of sea urchin (Lytechinus pictus) embryos. 海胆（Lytechinus pictus）胚胎的自动化、高通量原位杂交。

IF 3.6 2区生物学

Development Pub Date : 2025-09-15 Epub Date: 2025-09-22 DOI: 10.1242/dev.204814

Yoon Lee, Chloe Jenniches, Rachel Metry, Gloria Renaudin, Svenja Kling, Evan Tjeerdema, Elliot W Jackson, Amro Hamdoun

{"title":"Automated, high-throughput in situ hybridization of sea urchin (Lytechinus pictus) embryos.","authors":"Yoon Lee, Chloe Jenniches, Rachel Metry, Gloria Renaudin, Svenja Kling, Evan Tjeerdema, Elliot W Jackson, Amro Hamdoun","doi":"10.1242/dev.204814","DOIUrl":"10.1242/dev.204814","url":null,"abstract":"Despite the reach of in situ hybridization (ISH) in developmental biology, it is rarely used at scale. The major bottleneck is the throughput of the assay, which relies upon labor-intensive manual steps. The goal of this study was to develop a high-throughput, automated hybridization chain reaction (HCR) pipeline for the sea urchin (Lytechinus pictus). Our method, which we term high-throughput (HT)-HCR, can process 192 gene probe sets on whole-mount embryos within 32 h. The physical properties of sea urchin embryos enabled us to utilize a 96-well plate format, miniaturized reaction volumes, a general-purpose robotic liquid handler and automated confocal microscopy. Using this approach, we produced high quality localization data for 101 target genes across three developmental stages. The results reveal the localization of previously undescribed physiological genes, as well as canonical developmental transcription factors. HT-HCR represents an order of magnitude increase in the throughput of spatial expression profiling studies utilizing the sea urchin. This will enable more-sophisticated perturbation analyses and drug-screening efforts in this emerging animal model.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12516323/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145074587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transitions in development - an interview with Misty Riddle. 发展中的过渡——对Misty Riddle的采访。

IF 3.6 2区生物学

Development Pub Date : 2025-09-01 Epub Date: 2025-08-29 DOI: 10.1242/dev.205159

引用次数: 0

The people behind the papers - Maria Avdeeva, Madeline Chalifoux and Eszter Posfai. 这些报纸背后的人——玛丽亚·阿夫季娃，玛德琳·查利福和埃斯特·波斯特菲。

IF 3.6 2区生物学

Development Pub Date : 2025-09-01 Epub Date: 2025-09-05 DOI: 10.1242/dev.205160

引用次数: 0

The Company of Biologists and the British Society for Developmental Biology: a model partnership. 生物学家公司和英国发育生物学学会：一个模范的伙伴关系。

IF 3.6 2区生物学

Development Pub Date : 2025-09-01 Epub Date: 2025-09-15 DOI: 10.1242/dev.205152

Saanjbati Adhikari, Raman Das

引用次数: 0

Generative model for the first cell fate bifurcation in mammalian development. 哺乳动物发育中第一次细胞命运分叉的生成模型。

IF 3.6 2区生物学

Development Pub Date : 2025-09-01 Epub Date: 2025-09-05 DOI: 10.1242/dev.204717

Maria Avdeeva, Madeleine Chalifoux, Bradley Joyce, Stanislav Y Shvartsman, Eszter Posfai

{"title":"Generative model for the first cell fate bifurcation in mammalian development.","authors":"Maria Avdeeva, Madeleine Chalifoux, Bradley Joyce, Stanislav Y Shvartsman, Eszter Posfai","doi":"10.1242/dev.204717","DOIUrl":"10.1242/dev.204717","url":null,"abstract":"The first cell fate bifurcation in mammalian development directs cells toward either the trophectoderm (TE) or inner cell mass (ICM) compartments in pre-implantation embryos. This decision is regulated by the subcellular localization of a transcriptional co-activator YAP and takes place over several progressively asynchronous cleavage divisions. As a result of this asynchrony and variable arrangement of blastomeres, reconstructing the dynamics of the TE/ICM cell specification from fixed embryos is extremely challenging. To address this, we developed a live-imaging approach and applied it to measure pairwise dynamics of nuclear YAP and its direct target genes, CDX2 and SOX2, which are key transcription factors of the TE and ICM, respectively. Using these datasets, we constructed a generative model of the first cell fate bifurcation, which reveals the time-dependent statistics of the TE and ICM cell allocation. In addition to making testable predictions for the joint dynamics of the full YAP/CDX2/SOX2 motif, the model revealed the stochastic nature of the induction timing of the key cell fate determinants and identified the features of YAP dynamics that are necessary or sufficient for this induction. Notably, temporal heterogeneity was particularly prominent for SOX2 expression among ICM cells. As heterogeneities within the ICM have been linked to the initiation of the second cell fate decision in the embryo, understanding the origins of this variability is of key significance. The presented approach reveals the dynamics of the first cell fate choice and lays the groundwork for dissecting the next cell fate decisions in mouse development.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12327801/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144783787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TOR signalling mediates the collective movement of border cells in Drosophila oogenesis. TOR信号介导果蝇卵发生中边界细胞的集体运动。

IF 3.6 2区生物学

Development Pub Date : 2025-09-01 Epub Date: 2025-09-04 DOI: 10.1242/dev.204612

Sudipta Halder, Adhisree Sharma, Sayan Acharjee, Neha Biju, Mincy Kunjumon, Rohan Jayant Khadilkar, Mohit Prasad

引用次数: 0

Frozen potential: embryo research at the crossroads of ethics, regulation and scientific opportunity. 冷冻潜力：胚胎研究在伦理，监管和科学机会的十字路口。

IF 3.6 2区生物学

Development Pub Date : 2025-09-01 Epub Date: 2025-09-08 DOI: 10.1242/dev.205133

Mina Popovic, Catello Scarica, Susana M Chuva de Sousa Lopes, Marta N Shahbazi

{"title":"Frozen potential: embryo research at the crossroads of ethics, regulation and scientific opportunity.","authors":"Mina Popovic, Catello Scarica, Susana M Chuva de Sousa Lopes, Marta N Shahbazi","doi":"10.1242/dev.205133","DOIUrl":"10.1242/dev.205133","url":null,"abstract":"In an era of expanding reproductive possibilities, the human embryo has come to represent both immense potential and profound constraint. Advances in medically assisted reproduction (MAR) have led to the cryopreservation of hundreds of thousands of embryos each year, yet many remain unused and are ultimately discarded. Meanwhile, studies aimed at understanding infertility, early human development and preventing miscarriage continue to face significant barriers, with only a small fraction of embryos ever donated to research. This disconnect, shaped by regulatory ambiguity, raises a deeper question: is it more ethical to discard an embryo than to learn from it? This Perspective outlines the biological inefficiencies of human reproduction and the clinical imperative to improve MAR outcomes. We then examine the global patchwork of embryo research regulation by comparing national approaches. Drawing on examples from both clinical and research practice, we argue that permissiveness alone does not guarantee scientific progress, just as restriction does not ensure ethical integrity. A meaningful global conversation on embryo research must move beyond the binary of permissiveness versus prohibition, and toward frameworks that support responsible, transparent, and ethically grounded innovation.","PeriodicalId":11375,"journal":{"name":"Development","volume":"152 17","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145014142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Manifold roles of the chemokine G-protein-coupled receptor CCR7 in differentiation of human trophoblast into extravillous and syncytiotrophoblast lineages. CCR7趋化因子GPCR在人滋养细胞分化为胞外和合胞滋养细胞谱系中的多种作用。

IF 3.6 2区生物学

Development Pub Date : 2025-09-01 Epub Date: 2025-09-15 DOI: 10.1242/dev.204309

Eun-Ja Yoon, Mariana Beltcheva, Syed S Ali, Shuhua Fu, Amanda Yang, Chen Dong, Joseph E Zemke, Rowan M Karvas, Chia-Teng Chang, Laura A Fischer, Paul Gontarz, Bo Zhang, Sabine Dietmann, Thorold W Theunissen, Lilianna Solnica-Krezel

{"title":"Manifold roles of the chemokine G-protein-coupled receptor CCR7 in differentiation of human trophoblast into extravillous and syncytiotrophoblast lineages.","authors":"Eun-Ja Yoon, Mariana Beltcheva, Syed S Ali, Shuhua Fu, Amanda Yang, Chen Dong, Joseph E Zemke, Rowan M Karvas, Chia-Teng Chang, Laura A Fischer, Paul Gontarz, Bo Zhang, Sabine Dietmann, Thorold W Theunissen, Lilianna Solnica-Krezel","doi":"10.1242/dev.204309","DOIUrl":"10.1242/dev.204309","url":null,"abstract":"The chemokine G-protein-coupled receptor CCR7 is expressed in extra-embryonic tissues of the early human embryo, including trophectoderm and its derivatives: cytotrophoblast, extravillous trophoblast (EVT) and syncytiotrophoblast (STB). However, its function in placentation remains understudied. Here, we have generated human embryonic stem cells harboring CCR7 deletions and differentiated them into human trophoblast stem cells (hTSC), their EVT and STB derivatives, and trophoblast organoids. We found that CCR7 mutant hTSCs exhibited delayed EVT differentiation: they retained hTSC-like characteristics, and exhibited decreased epithelial-to-mesenchymal transition and cell motility. Investigation of trophoblast organoids using single cell transcriptomics showed that CCR7 mutant organoids comprised a smaller EVT, but a larger STB population, compared to wild type. Whereas CCR7 deficiency increased cell fusion during STB differentiation, excess CCR7 reduced expression of fusion-associated genes. Mechanistically, we found that CCR7 limited early STB differentiation by reducing cAMP levels. Transcriptional profiling of CCR7 mutant STBs identified reduced gene expression related to the placental viral defense. Together, our studies demonstrate that CCR7 plays multiple roles in cellular decision-making during trophoblast differentiation, promoting EVT differentiation and limiting cell fusion during early STB formation.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144947037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0