Development最新文献_第7页

An interview with Dominique Bergmann. 采访多米尼克·伯格曼。

IF 3.7 2区生物学

Development Pub Date : 2025-02-15 Epub Date: 2025-02-20 DOI: 10.1242/dev.204667

引用次数: 0

Spatio-temporal reconstruction of gene expression patterns in developing mice. 发育小鼠基因表达模式的时空重构。

IF 3.7 2区生物学

Development Pub Date : 2025-02-15 Epub Date: 2025-02-21 DOI: 10.1242/dev.204313

Laura Aviñó-Esteban, Heura Cardona-Blaya, James Sharpe

{"title":"Spatio-temporal reconstruction of gene expression patterns in developing mice.","authors":"Laura Aviñó-Esteban, Heura Cardona-Blaya, James Sharpe","doi":"10.1242/dev.204313","DOIUrl":"10.1242/dev.204313","url":null,"abstract":"Understanding gene regulation in organism development is crucial in biology. Techniques like whole-mount in situ hybridization can reveal spatial gene expression in organs and tissues. However, capturing time-lapse movies of gene expression dynamics in embryos developing in utero, such as mice, remains technically challenging beyond the early stages. To address this, we present a method to integrate static snapshots of gene expression patterns across limb developmental stages, creating a continuous 2D reconstruction of gene expression patterns over time. This method interpolates small tissue regions over time to create smooth temporal trajectories of gene expression. We successfully applied it to a number of key genes in limb development, including Sox9, Hand2, and Bmp2. This approach enables a detailed spatio-temporal mapping of gene expression, providing insights into developmental mechanisms. By estimating gene expression patterns at previously unobserved time points, it facilitates the comparison of these patterns across samples. The reconstructed trajectories offer high-quality data that will be useful to guide computational modeling and machine learning, advancing the study of developmental biology in systems where real-time imaging is technically difficult or impossible.","PeriodicalId":11375,"journal":{"name":"Development","volume":"152 4","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11883288/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143467312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Khdrbs1 drives re-differentiation of bipotential progenitor cells by inhibiting p53 in zebrafish biliary-mediated liver regeneration. Khdrbs1通过抑制p53在斑马鱼胆道介导的肝脏再生中驱动双电位祖细胞的再分化。

IF 3.7 2区生物学

Development Pub Date : 2025-02-15 Epub Date: 2025-02-28 DOI: 10.1242/dev.204266

Kai Gang, Qi Chen, Junhui Sun, Tingwei Zhang, Pengcheng Cai, Rui Ni, Jianlong Ma

{"title":"Khdrbs1 drives re-differentiation of bipotential progenitor cells by inhibiting p53 in zebrafish biliary-mediated liver regeneration.","authors":"Kai Gang, Qi Chen, Junhui Sun, Tingwei Zhang, Pengcheng Cai, Rui Ni, Jianlong Ma","doi":"10.1242/dev.204266","DOIUrl":"10.1242/dev.204266","url":null,"abstract":"After severe liver injury, biliary epithelial cells (BECs) undergo de-differentiation into bipotential progenitor cells (BPPCs), which subsequently re-differentiate into nascent hepatocytes and BECs to accomplish liver regeneration. However, the crucial factors governing the re-differentiation process of BPPCs remain largely unknown. Here, using a zebrafish model of severe liver injury, we observed specific expression of khdrbs1a and khdrbs1b (collectively known as khdrbs1) in BPPCs through single-cell RNA analyses and fluorescence in situ hybridization. Subsequently, to eliminate the genetic compensation, we generated a CRISPR/dead Cas9-mediated system for interfering with khdrbs1 in BECs, which caused defective liver regeneration and impaired re-differentiation of BPPCs. Furthermore, the khdrbs1-/- mutant displayed impaired proliferation and re-differentiation of BPPCs during liver regeneration. Mechanistically, p53 signaling was activated in response to the loss of khdrbs1, and tp53 mutation partially rescued the defective liver regeneration of the khdrbs1-/- mutant. In summary, we conclude that Khdrbs1 promotes the re-differentiation of BPPCs in part by inhibiting p53 activation during biliary-mediated liver regeneration in zebrafish.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11928054/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143440304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The people behind the papers - Laura Aviñó-Esteban and James Sharpe. 这些报纸背后的人——劳拉Aviñó-Esteban和詹姆斯夏普。

IF 3.7 2区生物学

Development Pub Date : 2025-02-15 Epub Date: 2025-02-21 DOI: 10.1242/dev.204707

引用次数: 0

Single cell-derived multicellular meristem: insights into male-to-hermaphrodite conversion and de novo meristem formation in Ceratopteris. 单细胞衍生的多细胞分生组织：角翅目雄性向雌雄同体转化和新生分生组织形成的见解。

IF 3.7 2区生物学

Development Pub Date : 2025-02-01 Epub Date: 2025-02-13 DOI: 10.1242/dev.204411

Xi Yang, An Yan, Xing Liu, Alexandria Volkening, Yun Zhou

{"title":"Single cell-derived multicellular meristem: insights into male-to-hermaphrodite conversion and de novo meristem formation in Ceratopteris.","authors":"Xi Yang, An Yan, Xing Liu, Alexandria Volkening, Yun Zhou","doi":"10.1242/dev.204411","DOIUrl":"10.1242/dev.204411","url":null,"abstract":"Land plants alternate between asexual sporophytes and sexual gametophytes. Unlike seed plants, ferns develop free-living gametophytes. Gametophytes of the model fern Ceratopteris exhibit two sex types: hermaphrodites with pluripotent meristems and males lacking meristems. In the absence of the pheromone antheridiogen, males convert to hermaphrodites by forming de novo meristems, although the mechanisms remain unclear. Using long-term time-lapse imaging and computational analyses, we captured male-to-hermaphrodite conversion at single-cell resolution and reconstructed the lineage and division atlas of newly formed meristems. Lineage tracing revealed that the de novo-formed meristem originates from a single non-antheridium cell: the meristem progenitor cell (MPC). During conversion, the MPC lineage showed increased mitotic activity, with marginal cells proliferating faster than inner cells. A mathematical model suggested that stochastic variation in cell division, combined with strong inhibitory signals from dividing marginal cells, is sufficient to explain gametophyte dynamics. Experimental disruption of division timing agreed with the model, showing that precise cell cycle progression is essential for MPC establishment and sex-type conversion. These findings reveal cellular mechanisms governing sex conversion and de novo meristem formation in land plants.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11883269/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143001979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transitions in development - an interview with Aleksandra Pękowska. 开发中的过渡——对Aleksandra的采访Pękowska。

IF 3.7 2区生物学

Development Pub Date : 2025-02-01 Epub Date: 2025-02-05 DOI: 10.1242/dev.204603

引用次数: 0

Conserved roles of ETT and ARF4 in gynoecium development in Brassicaceae with distinct fruit shapes. ETT和ARF4在具有不同果实形状的十字花科植物雌蕊群发育过程中的作用是一致的。

IF 3.7 2区生物学

Development Pub Date : 2025-02-01 Epub Date: 2025-02-12 DOI: 10.1242/dev.204263

Heather Marie McLaughlin, Tian-Feng Lü, Bhavani Natarajan, Lars Østergaard, Yang Dong

{"title":"Conserved roles of ETT and ARF4 in gynoecium development in Brassicaceae with distinct fruit shapes.","authors":"Heather Marie McLaughlin, Tian-Feng Lü, Bhavani Natarajan, Lars Østergaard, Yang Dong","doi":"10.1242/dev.204263","DOIUrl":"10.1242/dev.204263","url":null,"abstract":"Gynoecium patterning is dependent on the dynamic distribution of auxin, the signalling of which is transduced through several distinct pathways. ETTIN (ETT)-mediated signalling occurs independently of the canonical auxin pathway, and ETT shares partial redundancy with Auxin Response Factor 4 (ARF4) in the gynoecium. ETT and ARF4 were previously hypothesized to translate auxin gradients into patterns of tissue polarity alongside other ARFs. As ARF repressors, ETT/ARF were assumed to antagonistically regulate targets shared with ARF activators of the canonical pathway. Here, comparative transcriptomics identified the distinct and overlapping targets of ETT/ARF4 in the Arabidopsis gynoecium. However, ETT/ARF4 targets with known roles in gynoecium development did not conform to models of A-B ARF antagonism, leaving the relationship with the canonical pathway unclear. Mutants in tir1 afb2 ett were therefore generated in Arabidopsis and Capsella to assess the relationship between the two pathways, and their conservation in species with distinct fruit shapes. The data presented indicate conserved synergism between the two pathways in gynoecium development and suggest a role for ARF4 in the integration of these pathways in Brassicaceae with distinct fruit shapes.","PeriodicalId":11375,"journal":{"name":"Development","volume":"152 3","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11883277/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143398727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acute inflammation induces acute megakaryopoiesis with impaired platelet production during fetal hematopoiesis. 急性炎症诱导急性巨核生成，胎儿造血过程中血小板生成受损。

IF 3.7 2区生物学

Development Pub Date : 2025-02-01 Epub Date: 2025-02-05 DOI: 10.1242/dev.204226

Xiaojie Hu, Yirui He, Shengwei Li, Yue Jiang, Renjie Yu, Yi Wu, Xiaoying Fu, Yuanbin Song, Changdong Lin, Jiejun Shi, Hua-Bing Li, Yimeng Gao

{"title":"Acute inflammation induces acute megakaryopoiesis with impaired platelet production during fetal hematopoiesis.","authors":"Xiaojie Hu, Yirui He, Shengwei Li, Yue Jiang, Renjie Yu, Yi Wu, Xiaoying Fu, Yuanbin Song, Changdong Lin, Jiejun Shi, Hua-Bing Li, Yimeng Gao","doi":"10.1242/dev.204226","DOIUrl":"10.1242/dev.204226","url":null,"abstract":"Hematopoietic development is tightly regulated by various factors. The role of RNA m6A modification during fetal hematopoiesis, particularly in megakaryopoiesis, remains unclear. Here, we demonstrate that loss of m6A methyltransferase METTL3 induces formation of double-stranded RNAs (dsRNAs) and activates acute inflammation during fetal hematopoiesis in mouse. This dsRNA-mediated inflammation leads to acute megakaryopoiesis, which facilitates the generation of megakaryocyte progenitors but disrupts megakaryocyte maturation and platelet production. The inflammation and immune response activate the phosphorylation of STAT1 and IRF3, and upregulate downstream interferon-stimulated genes (ISGs). Inflammation inhibits the proliferation rate of hematopoietic progenitors and further skews the cell fate determination toward megakaryocytes rather than toward erythroid from megakaryocyte-erythroid progenitors (MEPs). Transcriptional-wide gene expression analysis identifies IGF1 as a major factor whose reduction is responsible for the inhibition of megakaryopoiesis and thrombopoiesis. Restoration of IGF1 with METTL3-deficient hematopoietic cells significantly increases megakaryocyte maturation. In summary, we elucidate that the loss of RNA m6A modification-induced acute inflammation activates acute megakaryopoiesis, but impairs its final maturation through the inhibition of IGF1 expression during fetal hematopoiesis.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143001973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An in vivo CRISPR screen in chick embryos reveals a role for MLLT3 in specification of neural cells from the caudal epiblast. 鸡胚胎的体内CRISPR筛选揭示了MLLT3在尾端外胚层神经细胞分化中的作用。

IF 3.7 2区生物学

Development Pub Date : 2025-02-01 Epub Date: 2025-02-12 DOI: 10.1242/dev.204591

Ashley R G Libby, Tiago Rito, Arthur Radley, James Briscoe

引用次数: 0

BMP and STRA8 act collaboratively to ensure correct mitotic-to-meiotic transition in the fetal mouse ovary. BMP和STRA8协同作用，确保胚胎小鼠卵巢中有丝分裂向减数分裂的正确转变。

IF 3.7 2区生物学

Development Pub Date : 2025-02-01 Epub Date: 2025-02-07 DOI: 10.1242/dev.204227

Fiona K M Cheung, Chun-Wei Allen Feng, Clare Crisp, Yuji Mishina, Cassy M Spiller, Josephine Bowles

引用次数: 0