Development最新文献_第7页

Single-cell transcriptomes reveal spermatogonial stem cells and the dynamic heterogeneity of spermatogenesis in a seasonal breeding teleost. 单细胞转录组揭示了一种季节性繁殖远洋鱼类的精原干细胞和精子发生的动态异质性。

IF 3.7 2区生物学

Development Pub Date : 2024-11-15 Epub Date: 2024-11-20 DOI: 10.1242/dev.203142

Yang Yang, Yinan Zhou, Gary Wessel, Weihua Hu, Dongdong Xu

{"title":"Single-cell transcriptomes reveal spermatogonial stem cells and the dynamic heterogeneity of spermatogenesis in a seasonal breeding teleost.","authors":"Yang Yang, Yinan Zhou, Gary Wessel, Weihua Hu, Dongdong Xu","doi":"10.1242/dev.203142","DOIUrl":"https://doi.org/10.1242/dev.203142","url":null,"abstract":"Seasonal spermatogenesis in fish is driven by spermatogonial stem cells (SSCs), which undergo a complex cellular process to differentiate into mature sperm. In this study, we characterized spermatogenesis in the large yellow croaker (Larimichthys crocea), a marine fish of significant commercial value, based on a high-resolution single-cell RNA-sequencing atlas of testicular cells from three distinct developmental stages: juvenile, adult differentiating and regressed testes. We detailed a continuous developmental trajectory of spermatogenic cells, from spermatogonia to spermatids, elucidating the molecular events involved in spermatogenesis. We uncovered dynamic heterogeneity in cellular compositions throughout the annual reproductive cycle, accompanied by strong molecular signatures within specific testicular cells. Notably, we identified a distinct population of SSCs and observed a critical metabolic transition from glycolysis to oxidative phosphorylation, enhancing our understanding of the biochemical and molecular characteristics of SSCs. Additionally, we elucidated the interactions between somatic cells and spermatogonia, illuminating the mechanisms that regulate SSC development. Overall, this work enhances our understanding of spermatogenesis in seasonal breeding teleosts and provides essential insights for the further conservation and culture of SSCs.","PeriodicalId":11375,"journal":{"name":"Development","volume":"151 22","pages":""},"PeriodicalIF":3.7,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142681238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Ednrb-Aim2-AKT axis regulates neural crest-derived melanoblast proliferation during early development. Ednrb-Aim2-AKT轴调节早期发育过程中神经嵴衍生的黑色母细胞增殖。

IF 3.7 2区生物学

Development Pub Date : 2024-11-15 Epub Date: 2024-11-18 DOI: 10.1242/dev.202444

Yu Chen, Huirong Li, Jing Wang, Shanshan Yang, Zhongyuan Su, Wanxiao Wang, Chunbao Rao, Ling Hou

{"title":"The Ednrb-Aim2-AKT axis regulates neural crest-derived melanoblast proliferation during early development.","authors":"Yu Chen, Huirong Li, Jing Wang, Shanshan Yang, Zhongyuan Su, Wanxiao Wang, Chunbao Rao, Ling Hou","doi":"10.1242/dev.202444","DOIUrl":"10.1242/dev.202444","url":null,"abstract":"Ednrb is specifically required to develop neural crest (NC) stem cell-derived lineages. However, it is still unknown why Ednrb signaling is only needed for the early development of melanoblasts in the skin and eye. We show that Ednrb is required for the proliferation of melanoblasts during early mouse development. To understand the mechanism of melanoblast proliferation, we found that the gene absent in melanoma 2 (Aim2) is upregulated in Ednrb-deficient NC cells by RNA-sequencing analysis. Consequently, the knockdown or knockout of Aim2 partially rescued the proliferation of Ednrb-deficient melanoblasts. Conversely, the overexpression of Aim2 in melanoblasts suppressed their proliferation. We further show that Ednrb signaling could act through the microRNA miR-196b to block the suppression of melanoblast proliferation by Aim2 in primary NC cell cultures. These results reveal the Ednrb-Aim2-AKT axis in regulating melanocyte development and suggest that Ednrb signaling functions as a negative regulator of Aim2, which inhibits the proliferation of melanoblasts in early development. These findings uncover a previously unreported role for Aim2 outside the inflammasome, showing that it is a significant regulator controlling NC stem cell-derived lineage development.","PeriodicalId":11375,"journal":{"name":"Development","volume":"151 22","pages":""},"PeriodicalIF":3.7,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142647662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hepatocyte differentiation requires anisotropic expansion of bile canaliculi. 肝细胞分化需要各向异性的胆管扩张。

IF 3.7 2区生物学

Development Pub Date : 2024-11-15 Epub Date: 2024-11-21 DOI: 10.1242/dev.202777

Maarten P Bebelman, Lenka Belicova, Elzbieta Gralinska, Tobias Jumel, Aparajita Lahree, Sarah Sommer, Andrej Shevchenko, Timofei Zatsepin, Yannis Kalaidzidis, Martin Vingron, Marino Zerial

{"title":"Hepatocyte differentiation requires anisotropic expansion of bile canaliculi.","authors":"Maarten P Bebelman, Lenka Belicova, Elzbieta Gralinska, Tobias Jumel, Aparajita Lahree, Sarah Sommer, Andrej Shevchenko, Timofei Zatsepin, Yannis Kalaidzidis, Martin Vingron, Marino Zerial","doi":"10.1242/dev.202777","DOIUrl":"10.1242/dev.202777","url":null,"abstract":"During liver development, bipotential progenitor cells called hepatoblasts differentiate into hepatocytes or cholangiocytes. Hepatocyte differentiation is uniquely associated with multi-axial polarity, enabling the anisotropic expansion of apical lumina between adjacent cells and formation of a three-dimensional network of bile canaliculi. Cholangiocytes, the cells forming the bile ducts, exhibit the vectorial polarity characteristic of epithelial cells. Whether cell polarization feeds back on the gene regulatory pathways governing hepatoblast differentiation is unknown. Here, we used primary mouse hepatoblasts to investigate the contribution of anisotropic apical expansion to hepatocyte differentiation. Silencing of the small GTPase Rab35 caused isotropic lumen expansion and formation of multicellular cysts with the vectorial polarity of cholangiocytes. Gene expression profiling revealed that these cells express reduced levels of hepatocyte markers and upregulate genes associated with cholangiocyte identity. Timecourse RNA sequencing demonstrated that loss of lumen anisotropy precedes these transcriptional changes. Independent alterations in apical lumen morphology induced either by modulation of the subapical actomyosin cortex or by increased intraluminal pressure caused similar transcriptional changes. These findings suggest that cell polarity and lumen morphogenesis feed back to hepatoblast-to-hepatocyte differentiation.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11607689/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142380271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Non-apoptotic caspase events and Atf3 expression underlie direct neuronal differentiation of adult neural stem cells. 成体神经干细胞的非凋亡性caspase事件和Atf3表达是神经元直接分化的基础。

IF 3.7 2区生物学

Development Pub Date : 2024-11-15 Epub Date: 2024-11-20 DOI: 10.1242/dev.204381

Frédéric Rosa, Nicolas Dray, Sébastien Bedu, Laure Bally-Cuif

{"title":"Non-apoptotic caspase events and Atf3 expression underlie direct neuronal differentiation of adult neural stem cells.","authors":"Frédéric Rosa, Nicolas Dray, Sébastien Bedu, Laure Bally-Cuif","doi":"10.1242/dev.204381","DOIUrl":"10.1242/dev.204381","url":null,"abstract":"Neural stem cells (NSCs) generate neurons over a lifetime in adult vertebrate brains. In the adult zebrafish pallium, NSCs persist long term through balanced fate decisions. These decisions include direct neuronal conversions, i.e. delamination and neurogenesis without a division. To characterize this process, we reanalyze intravital imaging data of adult pallial NSCs, and observe shared delamination dynamics between NSCs and committed neuronal progenitors. Searching for mechanisms predicting direct NSC conversions, we build an NSC-specific genetic tracer of Caspase3/7 activation (Cas3*/Cas7*) in vivo. We show that non-apoptotic Cas3*/7* events occur in adult NSCs and are biased towards lineage termination under physiological conditions, with a predominant generation of single neurons. We further identify the transcription factor Atf3 as necessary for this bias. Finally, we show that the Cas3*/7* pathway is engaged by NSCs upon parenchymal lesion and correlates with NSCs more prone to lineage termination and neuron formation. These results provide evidence for non-apoptotic caspase events occurring in vertebrate adult NSCs and link these events with the NSC fate decision of direct conversion, which is important for long-term NSC population homeostasis.","PeriodicalId":11375,"journal":{"name":"Development","volume":"151 22","pages":""},"PeriodicalIF":3.7,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11607687/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142675453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A neuro-lymphatic communication guides lymphatic development by CXCL12 and CXCR4 signaling. 神经-淋巴通讯通过 CXCL12/CXCR4 信号引导淋巴发育。

IF 3.7 2区生物学

Development Pub Date : 2024-11-15 Epub Date: 2024-11-26 DOI: 10.1242/dev.202901

Long Nguyen Hoang Do, Esteban Delgado, Casey G Lim, Meriem Bkhache, Amanda M Peluzzo, Yiming Hua, Manisha Oza, Sadia Mohsin, Hong Chen, Michael V Autieri, Seonhee Kim, Xiaolei Liu

{"title":"A neuro-lymphatic communication guides lymphatic development by CXCL12 and CXCR4 signaling.","authors":"Long Nguyen Hoang Do, Esteban Delgado, Casey G Lim, Meriem Bkhache, Amanda M Peluzzo, Yiming Hua, Manisha Oza, Sadia Mohsin, Hong Chen, Michael V Autieri, Seonhee Kim, Xiaolei Liu","doi":"10.1242/dev.202901","DOIUrl":"10.1242/dev.202901","url":null,"abstract":"Lymphatic vessels grow through active sprouting and mature into a vascular complex that includes lymphatic capillaries and collecting vessels that ensure fluid transport. However, the signaling cues that direct lymphatic sprouting and patterning remain unclear. In this study, we demonstrate that chemokine signaling, specifically through CXCL12 and CXCR4, plays crucial roles in regulating lymphatic development. We show that LEC-specific Cxcr4-deficient mouse embryos and CXCL12 mutant embryos exhibit severe defects in lymphatic sprouting, migration and lymphatic valve formation. We also discovered that CXCL12, originating from peripheral nerves, directs the migration of dermal lymphatic vessels to align with nerves in developing skin. Deletion of Cxcr4 or blockage of CXCL12 and CXCR4 activity results in reduced VEGFR3 levels on the LEC surface. This, in turn, impairs VEGFC-mediated VEGFR3 signaling and downstream PI3K and AKT activities. Taken together, these data identify previously unknown chemokine signaling originating from peripheral nerves that guides dermal lymphatic sprouting and patterning. Our work identifies for the first time a neuro-lymphatics communication during mouse development and reveals a previously unreported mechanism by which CXCR4 modulates VEGFC, VEGFR3 and AKT signaling.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11634036/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142521313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The people behind the papers - Maarten Bebelman, Lenka Belicova and Marino Zerial. 报纸背后的人物 - Maarten Bebelman、Lenka Belicova 和 Marino Zerial。

IF 3.7 2区生物学

Development Pub Date : 2024-11-15 Epub Date: 2024-11-21 DOI: 10.1242/dev.204486

引用次数: 0

An expanded view of cell competition. 细胞竞争的扩展视角。

IF 3.7 2区生物学

Development Pub Date : 2024-11-15 Epub Date: 2024-11-19 DOI: 10.1242/dev.204212

Ameya Khandekar, Stephanie J Ellis

引用次数: 0

Ernest Arenas (1962-2024): a legacy of advancing dopaminergic neurogenesis research. Ernest Arenas（1962-2024 年）：推动多巴胺能神经发生研究的遗产。

IF 3.7 2区生物学

Development Pub Date : 2024-11-15 Epub Date: 2024-11-13 DOI: 10.1242/dev.204459

Carlos Villaescusa

引用次数: 0

Differential vegfc expression dictates lymphatic response during zebrafish heart development and regeneration. 斑马鱼心脏发育和再生过程中不同的 vegfc 表达决定了淋巴反应。

IF 3.7 2区生物学

Development Pub Date : 2024-11-15 Epub Date: 2024-11-19 DOI: 10.1242/dev.202947

Sierra Duca, Yu Xia, Laila Abd Elmagid, Isaac Bakis, Miaoyan Qiu, Yingxi Cao, Ylan Guo, James V Eichenbaum, Megan L McCain, Junsu Kang, Michael R M Harrison, Jingli Cao

{"title":"Differential vegfc expression dictates lymphatic response during zebrafish heart development and regeneration.","authors":"Sierra Duca, Yu Xia, Laila Abd Elmagid, Isaac Bakis, Miaoyan Qiu, Yingxi Cao, Ylan Guo, James V Eichenbaum, Megan L McCain, Junsu Kang, Michael R M Harrison, Jingli Cao","doi":"10.1242/dev.202947","DOIUrl":"10.1242/dev.202947","url":null,"abstract":"Vascular endothelial growth factor C (Vegfc) is crucial for lymphatic and blood vessel development, yet its cellular sources and specific functions in heart development remain unclear. To address this, we created a vegfc reporter and an inducible overexpression line in zebrafish. We found vegfc expression in large coronary arteries, circulating thrombocytes, cardiac adipocytes, and outflow tract smooth muscle cells. Notably, although coronary lymphangiogenesis aligns with Vegfc-expressing arteries in juveniles, it occurs only after coronary artery formation. Vegfc overexpression induced ectopic lymphatics on the ventricular surface prior to arterial formation, indicating that Vegfc abundance, rather than arterial presence, drives lymphatic development. However, this overexpression did not affect coronary artery coverage, suggesting a specific role for Vegfc in lymphatic, rather than arterial, development. Thrombocytes emerged as the initial Vegfc source during inflammation following heart injuries, transitioning to endocardial and myocardial expression during regeneration. Lower Vegfc levels in an amputation model corresponded with a lack of lymphatic expansion. Importantly, Vegfc overexpression enhanced lymphatic expansion and promoted scar resolution without affecting cardiomyocyte proliferation, highlighting its role in regulating lymphangiogenesis and promoting heart regeneration.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11607685/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142603757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SLC25A1 regulates placental development to ensure embryonic heart morphogenesis. SLC25A1 调节胎盘发育，确保胚胎心脏的形态形成。

IF 3.7 2区生物学

Development Pub Date : 2024-11-15 Epub Date: 2024-11-26 DOI: 10.1242/dev.204290

Wenli Fan, Zixuan Li, Xueke He, Xiaodong Wang, Ming Sun, Zhongzhou Yang

{"title":"SLC25A1 regulates placental development to ensure embryonic heart morphogenesis.","authors":"Wenli Fan, Zixuan Li, Xueke He, Xiaodong Wang, Ming Sun, Zhongzhou Yang","doi":"10.1242/dev.204290","DOIUrl":"https://doi.org/10.1242/dev.204290","url":null,"abstract":"22q11.2 deletion syndrome (22q11.2DS) is the most common chromosomal microdeletion syndrome. Congenital heart defects are prevalent in 22q11.2DS but the etiology is still poorly understood. In this study, we aimed to gain mechanistic insights into the heart defects that result from 22q11.2 deletion, with a focus on Slc25a1, which is located in the deletion segment. Whereas global knockout of Slc25a1 in mice produced a variety of heart malformations, cardiac deletion of Slc25a1 had little effect on heart development. We then found that trophoblast-specific Slc25a1 deletion recapitulated heart anomalies in the global knockout mice. Further study identified SLC25A1 as a regulator of trophoblast and placental development through modulation of histone H3K27 acetylation at the promoters and enhancers of key genes involved in trophoblast differentiation. Finally, administration of recombinant human pregnancy-specific glycoprotein 1 (PSG1), a trophoblast-derived secretory glycoprotein, partially corrected placental and embryonic heart defects. This study defines the role of SLC25A1 in heart development by regulating placental development, and provides new insights to understand the etiology of 22q11.2DS.","PeriodicalId":11375,"journal":{"name":"Development","volume":"151 22","pages":""},"PeriodicalIF":3.7,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142727058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0