Development最新文献_第6页

An Lgr5-independent developmental lineage is involved in mouse intestinal regeneration. 一个不依赖lgr5的发育谱系参与了小鼠肠道再生。

IF 3.7 2区生物学

Development Pub Date : 2025-02-27 DOI: 10.1242/dev.204654

Maryam Marefati, Valeria Fernandez-Vallone, Morgane Leprovots, Gabriella Vasile, Frédérick Libert, Anne Lefort, Gilles Dinsart, Achim Weber, Jasna Jetzer, Marie-Isabelle Garcia, Gilbert Vassart

引用次数: 0

Mechanical strain focusing at topological defect sites in regenerating Hydra. 水螅再生中拓扑缺陷部位的机械应变聚焦。

IF 3.7 2区生物学

Development Pub Date : 2025-02-15 Epub Date: 2025-03-03 DOI: 10.1242/dev.204514

Yonit Maroudas-Sacks, S Suganthan, Liora Garion, Yael Ascoli-Abbina, Ariel Westfried, Noam Dori, Iris Pasvinter, Marko Popović, Kinneret Keren

{"title":"Mechanical strain focusing at topological defect sites in regenerating Hydra.","authors":"Yonit Maroudas-Sacks, S Suganthan, Liora Garion, Yael Ascoli-Abbina, Ariel Westfried, Noam Dori, Iris Pasvinter, Marko Popović, Kinneret Keren","doi":"10.1242/dev.204514","DOIUrl":"10.1242/dev.204514","url":null,"abstract":"The formation of a new head during Hydra regeneration involves the establishment of a head organizer that functions as a signaling center and contains an aster-shaped topological defect in the organization of the supracellular actomyosin fibers. Here, we show that the future head region in regenerating tissue fragments undergoes multiple instances of extensive stretching and rupture events from the onset of regeneration. These recurring localized tissue deformations arise due to transient contractions of the supracellular ectodermal actomyosin fibers that focus mechanical strain at defect sites. We further show that stabilization of aster-shaped defects is disrupted by perturbations of the Wnt signaling pathway. We propose a closed-loop feedback mechanism promoting head organizer formation, and develop a biophysical model of regenerating Hydra tissues that incorporates a morphogen source activated by mechanical strain and an alignment interaction directing fibers along morphogen gradients. We suggest that this positive-feedback loop leads to mechanical strain focusing at defect sites, enhancing local morphogen production and promoting robust organizer formation.","PeriodicalId":11375,"journal":{"name":"Development","volume":"152 4","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11925399/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143536809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Machine learning approaches for image classification in developmental biology and clinical embryology. 发育生物学和临床胚胎学中图像分类的机器学习方法。

IF 3.7 2区生物学

Development Pub Date : 2025-02-15 Epub Date: 2025-02-17 DOI: 10.1242/dev.202066

Camilla Mapstone, Berenika Plusa

引用次数: 0

Dendritic cells in developing and adult zebrafish arise from different origins and display distinct flt3 dependencies. 发育中的斑马鱼和成年斑马鱼的树突状细胞起源于不同的起源，并表现出不同的细胞依赖性。

IF 3.7 2区生物学

Development Pub Date : 2025-02-15 Epub Date: 2025-02-17 DOI: 10.1242/dev.204410

Guanzhen Lin, Youqi Wang, Thi Giang Pham, Zilong Wen

引用次数: 0

The people behind the papers - Yonit Maroudas-Sacks and Marko Popović. 报纸背后的人-尤尼特·马鲁达斯-萨克斯和马尔科·波波维奇。

IF 3.7 2区生物学

Development Pub Date : 2025-02-15 Epub Date: 2025-03-03 DOI: 10.1242/dev.204655

引用次数: 0

Autonomous function of Antennapedia in adult muscle precursors directly connects Hox genes to adult muscle development. 成年肌肉前体中触角基（Antp）的自主功能直接将Hox基因与成年肌肉发育联系起来。

IF 3.7 2区生物学

Development Pub Date : 2025-02-15 Epub Date: 2025-02-17 DOI: 10.1242/dev.204341

Aakriti Singh, Meike van den Burgh, Vigneshwarr Boopathy, Patrick van Nierop Y Sanchez, Josephine Bageritz, Ingrid Lohmann, Katrin Domsch

{"title":"Autonomous function of Antennapedia in adult muscle precursors directly connects Hox genes to adult muscle development.","authors":"Aakriti Singh, Meike van den Burgh, Vigneshwarr Boopathy, Patrick van Nierop Y Sanchez, Josephine Bageritz, Ingrid Lohmann, Katrin Domsch","doi":"10.1242/dev.204341","DOIUrl":"10.1242/dev.204341","url":null,"abstract":"The evolutionarily conserved Hox genes define segment identities along the anterior-posterior axis and are expressed in most cell types within each segment, performing specific functions tailored to cellular needs. It has been suggested previously that Drosophila adult flight muscles in the second thoracic segment (T2) develop without direct Hox gene input, relying instead on ectodermal signals to shape their identity. However, our research, leveraging single-cell transcriptomics of Drosophila wing discs and Hox perturbation experiments using CRISPR technology and gain-of-function assays, unveiled a more intricate regulatory landscape. We found that the Hox protein Antennapedia (Antp) is essential for adult flight muscle development, acting in two crucial ways: by regulating the cell cycle rate of adult muscle precursors (AMPs) through repression of proliferation genes, and by guiding flight muscle fate via regulation of Hedgehog (Hh) signalling during cell fate establishment. Antp, along with its co-factor Apterous (Ap), directly interacts with the patched (ptc) locus to control its expression in AMPs. These findings challenge the notion of T2 as a 'Hox-free' zone, highlighting the indispensable role of low-level Antp expression in adult muscle development.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143370453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A cardiac transcriptional enhancer is repurposed during regeneration to activate an anti-proliferative program. 心脏转录增强子在再生过程中被重新利用以激活抗增殖程序。

IF 3.7 2区生物学

Development Pub Date : 2025-02-15 Epub Date: 2025-02-17 DOI: 10.1242/dev.204458

Anupama Rao, Andrew Russell, Jose Segura-Bermudez, Charles Franz, Rejenae Dockery, Anton Blatnik, Jacob Panten, Mateo Zevallos, Carson McNulty, Maciej Pietrzak, Joseph Aaron Goldman

{"title":"A cardiac transcriptional enhancer is repurposed during regeneration to activate an anti-proliferative program.","authors":"Anupama Rao, Andrew Russell, Jose Segura-Bermudez, Charles Franz, Rejenae Dockery, Anton Blatnik, Jacob Panten, Mateo Zevallos, Carson McNulty, Maciej Pietrzak, Joseph Aaron Goldman","doi":"10.1242/dev.204458","DOIUrl":"10.1242/dev.204458","url":null,"abstract":"Zebrafish have a high capacity to regenerate their hearts. Several studies have surveyed transcriptional enhancers to understand how gene expression is controlled during heart regeneration. We have identified REN (the runx1 enhancer) that, during regeneration, regulates the expression of the nearby runx1 gene. We show that runx1 mRNA is reduced with deletion of REN (ΔREN), and cardiomyocyte proliferation is enhanced in ΔREN mutants only during regeneration. Interestingly, in uninjured hearts, ΔREN mutants have reduced expression of adamts1, a nearby gene that encodes a Collagen protease. This results in excess Collagen within cardiac valves of uninjured hearts. The ΔREN Collagen phenotype is rescued by an allele with Δrunx1 mutations, suggesting that in uninjured hearts REN regulates adamts1 independently of runx1. Taken together, this suggests that REN is rewired from adamts1 in uninjured hearts to stimulate runx1 transcription during regeneration. Our data point to a previously unappreciated mechanism for gene regulation during zebrafish heart regeneration. We report that an enhancer is rewired from expression in a distal cardiac domain to activate a different gene in regenerating tissue.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11883283/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142970054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In preprints: insights into the regulation of haematopoietic stem cell emergence in vitro and in vivo. 预印本：对体外和体内造血干细胞出现的调控的见解。

IF 3.7 2区生物学

Development Pub Date : 2025-02-15 Epub Date: 2025-02-20 DOI: 10.1242/dev.204689

George Hunt, Ana Cvejic

引用次数: 0

Cdkn1c orchestrates a molecular network that regulates euploidy of male mouse germline stem cells. Cdkn1c协调了一个调节雄性小鼠种系干细胞整倍性的分子网络。

IF 3.7 2区生物学

Development Pub Date : 2025-02-15 Epub Date: 2025-02-24 DOI: 10.1242/dev.204286

Mito Kanatsu-Shinohara, Takuya Yamamoto, Tianjiao Liu, Keiichi I Nakayama, Takashi Shinohara

{"title":"Cdkn1c orchestrates a molecular network that regulates euploidy of male mouse germline stem cells.","authors":"Mito Kanatsu-Shinohara, Takuya Yamamoto, Tianjiao Liu, Keiichi I Nakayama, Takashi Shinohara","doi":"10.1242/dev.204286","DOIUrl":"10.1242/dev.204286","url":null,"abstract":"Karyotype instability in the germline leads to infertility. Unlike the female germline, the male germline continuously produces fertile sperm throughout life. Here, we present a molecular network responsible for maintaining karyotype stability in the male mouse germline. Loss of the cyclin-dependent kinase inhibitor Cdkn1c in undifferentiated spermatogonia induced degeneration of spermatogenesis prior to entry into the differentiating spermatogonia stage. In vitro analysis of mouse spermatogonial stem cells revealed that CDKN1C localized to spindle microtubules during metaphase, and that disrupted microtubule dynamics increased its phosphorylation. Cdkn1c deficiency activated the spindle assembly checkpoint and led to centrosome amplification, premature chromosome segregation, and loss of AURKB, and ultimately TRP53-dependent apoptosis. Trp53-deficient spermatogonial stem cells exhibited karyotype defects, but proliferated normally despite reduced CDKN1C and AURKB expression. In contrast, Aurkb depletion upregulated TRP53 and CDKN1C, suggesting a negative feedback loop to maintain euploidy. Thus, Cdkn1c regulates the male germline karyotype.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143028321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dynamic map illuminates Hippo-cMyc module crosstalk driving cardiomyocyte proliferation. 动态图阐明hipo - cmyc模块串扰驱动心肌细胞增殖。

IF 3.7 2区生物学

Development Pub Date : 2025-02-15 Epub Date: 2025-02-17 DOI: 10.1242/dev.204397

Bryana N Harris, Laura A Woo, R Noah Perry, Alexia M Wallace, Mete Civelek, Matthew J Wolf, Jeffrey J Saucerman

引用次数: 0