Development最新文献_第6页

The role of TEAD4 in trophectoderm commitment and development is not conserved in non-rodent mammals. TEAD4在非啮齿类哺乳动物的滋养层外胚层承诺和发育中的作用并不保守。

IF 3.7 2区生物学

Development Pub Date : 2024-10-15 Epub Date: 2024-09-24 DOI: 10.1242/dev.202993

Alba Pérez-Gómez, Leopoldo González-Brusi, Inés Flores-Borobia, Beatriz Galiano-Cogolludo, Ismael Lamas-Toranzo, Julieta G Hamze, Adolfo Toledano-Díaz, Julián Santiago-Moreno, Priscila Ramos-Ibeas, Pablo Bermejo-Álvarez

{"title":"The role of TEAD4 in trophectoderm commitment and development is not conserved in non-rodent mammals.","authors":"Alba Pérez-Gómez, Leopoldo González-Brusi, Inés Flores-Borobia, Beatriz Galiano-Cogolludo, Ismael Lamas-Toranzo, Julieta G Hamze, Adolfo Toledano-Díaz, Julián Santiago-Moreno, Priscila Ramos-Ibeas, Pablo Bermejo-Álvarez","doi":"10.1242/dev.202993","DOIUrl":"10.1242/dev.202993","url":null,"abstract":"The first lineage differentiation in mammals gives rise to the inner cell mass and the trophectoderm (TE). In mice, TEAD4 is a master regulator of TE commitment, as it regulates the expression of other TE-specific genes and its ablation prevents blastocyst formation, but its role in other mammals remains unclear. Herein, we have observed that TEAD4 ablation in two phylogenetically distant species (bovine and rabbit) does not impede TE differentiation, blastocyst formation and the expression of TE markers, such as GATA3 and CDX2, although a reduced number of cells in the inner cell mass was observed in bovine TEAD4 knockout (KO) blastocysts. Transcriptional analysis in bovine blastocysts revealed no major transcriptional effect of the ablation, although the expression of hypoblast and Hippo signalling-related genes tended to be decreased in KO embryos. Experiments were conducted in the bovine model to determine whether TEAD4 was required for post-hatching development. TEAD4 KO spherical conceptuses showed normal development of the embryonic disc and TE, but hypoblast migration rate was reduced. At later stages of development (tubular conceptuses), no differences were observed between KO and wild-type conceptuses.","PeriodicalId":11375,"journal":{"name":"Development","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11463960/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142016667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Something old, something new: the origins of an unusual renal cell underpinning a beetle water-conserving mechanism. 新旧交替：甲虫节水机制所依赖的不寻常肾细胞的起源。

IF 3.7 2区生物学

Development Pub Date : 2024-10-15 Epub Date: 2024-10-28 DOI: 10.1242/dev.202994

Robin Beaven, Takashi Koyama, Muhammad T Naseem, Kenneth V Halberg, Barry Denholm

{"title":"Something old, something new: the origins of an unusual renal cell underpinning a beetle water-conserving mechanism.","authors":"Robin Beaven, Takashi Koyama, Muhammad T Naseem, Kenneth V Halberg, Barry Denholm","doi":"10.1242/dev.202994","DOIUrl":"10.1242/dev.202994","url":null,"abstract":"Tenebrionid beetles have been highly successful in colonising environments where water is scarce, underpinned by their unique osmoregulatory adaptations. These include a cryptonephridial arrangement of their organs, in which part of their renal/Malpighian tubules are bound to the surface of the rectum. Within the cryptonephridial tubules, an unusual cell type, the leptophragmata, plays a key physiological role underpinning water conservation. Nothing was known about the developmental mechanisms or evolution of these unusual renal cells. Here, we investigate mechanisms underpinning leptophragmata development in Tribolium castaneum. We find that leptophragmata express and require the Tiptop transcription factor, similar to secondary renal cells in Drosophila melanogaster, which express Teashirt and Tiptop, despite Drosophila lacking a crypronephridial arrangement. An additional transcription factor, Dachshund, is required to establish leptophragmata identity and to distinguish them from the secondary cells in the non-cryptonephridial region of renal tubule of Tribolium. Dachshund is also expressed in a sub-population of secondary cells in Drosophila. Leptophragmata, which are unique to the beetle lineage, appear to have originated from a specific renal cell type present ancestrally and to be specified by a conserved repertoire of transcription factors.","PeriodicalId":11375,"journal":{"name":"Development","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142460559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advancing stem cell technologies for conservation of wildlife biodiversity. 推进干细胞技术，保护野生生物多样性。

IF 3.7 2区生物学

Development Pub Date : 2024-10-15 Epub Date: 2024-10-09 DOI: 10.1242/dev.203116

Ashlee M Hutchinson, Ruth Appeltant, Tom Burdon, Qiuye Bao, Rhishikesh Bargaje, Andrea Bodnar, Stuart Chambers, Pierre Comizzoli, Laura Cook, Yoshinori Endo, Bob Harman, Katsuhiko Hayashi, Thomas Hildebrandt, Marisa L Korody, Uma Lakshmipathy, Jeanne F Loring, Clara Munger, Alex H M Ng, Ben Novak, Manabu Onuma, Sara Ord, Monique Paris, Andrew J Pask, Francisco Pelegri, Martin Pera, Ryan Phelan, Benyamin Rosental, Oliver A Ryder, Woranop Sukparangsi, Gareth Sullivan, Nicole Liling Tay, Nikki Traylor-Knowles, Shawn Walker, Antonia Weberling, Deanne J Whitworth, Suzannah A Williams, Jessye Wojtusik, Jun Wu, Qi-Long Ying, Thomas P Zwaka, Timo N Kohler

{"title":"Advancing stem cell technologies for conservation of wildlife biodiversity.","authors":"Ashlee M Hutchinson, Ruth Appeltant, Tom Burdon, Qiuye Bao, Rhishikesh Bargaje, Andrea Bodnar, Stuart Chambers, Pierre Comizzoli, Laura Cook, Yoshinori Endo, Bob Harman, Katsuhiko Hayashi, Thomas Hildebrandt, Marisa L Korody, Uma Lakshmipathy, Jeanne F Loring, Clara Munger, Alex H M Ng, Ben Novak, Manabu Onuma, Sara Ord, Monique Paris, Andrew J Pask, Francisco Pelegri, Martin Pera, Ryan Phelan, Benyamin Rosental, Oliver A Ryder, Woranop Sukparangsi, Gareth Sullivan, Nicole Liling Tay, Nikki Traylor-Knowles, Shawn Walker, Antonia Weberling, Deanne J Whitworth, Suzannah A Williams, Jessye Wojtusik, Jun Wu, Qi-Long Ying, Thomas P Zwaka, Timo N Kohler","doi":"10.1242/dev.203116","DOIUrl":"10.1242/dev.203116","url":null,"abstract":"Wildlife biodiversity is essential for healthy, resilient and sustainable ecosystems. For biologists, this diversity also represents a treasure trove of genetic, molecular and developmental mechanisms that deepen our understanding of the origins and rules of life. However, the rapid decline in biodiversity reported recently foreshadows a potentially catastrophic collapse of many important ecosystems and the associated irreversible loss of many forms of life on our planet. Immediate action by conservationists of all stripes is required to avert this disaster. In this Spotlight, we draw together insights and proposals discussed at a recent workshop hosted by Revive & Restore, which gathered experts to discuss how stem cell technologies can support traditional conservation techniques and help protect animal biodiversity. We discuss reprogramming, in vitro gametogenesis, disease modelling and embryo modelling, and we highlight the prospects for leveraging stem cell technologies beyond mammalian species.","PeriodicalId":11375,"journal":{"name":"Development","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11491813/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142388895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An interview with Liam Dolan. 采访 Liam Dolan。

IF 3.7 2区生物学

Development Pub Date : 2024-10-15 Epub Date: 2024-10-28 DOI: 10.1242/dev.204440

引用次数: 0

Microtubules and actin filaments direct nuclear movement during the polarisation of Marchantia spore cells. 微管和肌动蛋白丝在马氏囊孢子细胞极化过程中指导核运动。

IF 3.7 2区生物学

Development Pub Date : 2024-10-15 Epub Date: 2024-07-23 DOI: 10.1242/dev.202823

Sarah T Attrill, Hugh Mulvey, Clément Champion, Liam Dolan

{"title":"Microtubules and actin filaments direct nuclear movement during the polarisation of Marchantia spore cells.","authors":"Sarah T Attrill, Hugh Mulvey, Clément Champion, Liam Dolan","doi":"10.1242/dev.202823","DOIUrl":"https://doi.org/10.1242/dev.202823","url":null,"abstract":"The multicellular haploid stage of land plants develops from a single haploid cell produced by meiosis - the spore. Starting from a non-polar state, these spores develop polarity, divide asymmetrically and establish the first axis of symmetry. Here, we show that the nucleus migrates from the cell centroid to the basal pole during polarisation of the Marchantia polymorpha spore cell. A microtubule organising centre on the leading edge of the nucleus initiates a microtubule array between the nuclear surface and the cortex at the basal pole. Simultaneously, cortical microtubules disappear from the apical hemisphere but persist in the basal hemisphere. This is accompanied by the formation a dense network of fine actin filaments between the nucleus and the basal pole cortex. Experimental depolymerisation of either microtubules or actin filaments disrupts cellular asymmetry. These data demonstrate that the cytoskeleton reorganises during spore polarisation and controls the directed migration of the nucleus to the basal pole. The presence of the nucleus at the basal pole provides the cellular asymmetry for the asymmetric cell division that establishes the apical-basal axis of the plant.","PeriodicalId":11375,"journal":{"name":"Development","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141747660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adhesion and shrinkage transform the rounded pupal horn into an angular adult horn in Japanese rhinoceros beetle. 粘附和收缩将日本犀角金龟的圆形蛹角转化为角形成虫角。

IF 4.6 2区生物学

Development Pub Date : 2024-10-15 Epub Date: 2024-03-13 DOI: 10.1242/dev.202082

Keisuke Matsuda, Haruhiko Adachi, Hiroki Gotoh, Yasuhiro Inoue, Shigeru Kondo

引用次数: 0

Dissecting the dynamic cellular transcriptional atlas of adult teleost testis development throughout the annual reproductive cycle. 剖析成鱼睾丸在整个年度生殖周期中的动态细胞转录图谱。

IF 3.7 2区生物学

Development Pub Date : 2024-10-15 Epub Date: 2024-03-13 DOI: 10.1242/dev.202296

Chaofan Jin, Kai Yan, Mengya Wang, Weihao Song, Bo Wang, Yu Men, Jingjing Niu, Yan He, Quanqi Zhang, Jie Qi

{"title":"Dissecting the dynamic cellular transcriptional atlas of adult teleost testis development throughout the annual reproductive cycle.","authors":"Chaofan Jin, Kai Yan, Mengya Wang, Weihao Song, Bo Wang, Yu Men, Jingjing Niu, Yan He, Quanqi Zhang, Jie Qi","doi":"10.1242/dev.202296","DOIUrl":"10.1242/dev.202296","url":null,"abstract":"Teleost testis development during the annual cycle involves dramatic changes in cellular compositions and molecular events. In this study, the testicular cells derived from adult black rockfish at distinct stages - regressed, regenerating and differentiating - were meticulously dissected via single-cell transcriptome sequencing. A continuous developmental trajectory of spermatogenic cells, from spermatogonia to spermatids, was delineated, elucidating the molecular events involved in spermatogenesis. Subsequently, the dynamic regulation of gene expression associated with spermatogonia proliferation and differentiation was observed across spermatogonia subgroups and developmental stages. A bioenergetic transition from glycolysis to mitochondrial respiration of spermatogonia during the annual developmental cycle was demonstrated, and a deeper level of heterogeneity and molecular characteristics was revealed by re-clustering analysis. Additionally, the developmental trajectory of Sertoli cells was delineated, alongside the divergence of Leydig cells and macrophages. Moreover, the interaction network between testicular micro-environment somatic cells and spermatogenic cells was established. Overall, our study provides detailed information on both germ and somatic cells within teleost testes during the annual reproductive cycle, which lays the foundation for spermatogenesis regulation and germplasm preservation of endangered species.","PeriodicalId":11375,"journal":{"name":"Development","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140109573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interactions between U and V sex chromosomes during the life cycle of Ectocarpus. 外果藻生命周期中 U 和 V 性染色体之间的相互作用

IF 4.6 2区生物学

Development Pub Date : 2024-10-15 Epub Date: 2024-04-11 DOI: 10.1242/dev.202677

Jeromine Vigneau, Claudia Martinho, Olivier Godfroy, Min Zheng, Fabian B Haas, Michael Borg, Susana M Coelho

{"title":"Interactions between U and V sex chromosomes during the life cycle of Ectocarpus.","authors":"Jeromine Vigneau, Claudia Martinho, Olivier Godfroy, Min Zheng, Fabian B Haas, Michael Borg, Susana M Coelho","doi":"10.1242/dev.202677","DOIUrl":"10.1242/dev.202677","url":null,"abstract":"In many animals and flowering plants, sex determination occurs in the diploid phase of the life cycle with XX/XY or ZW/ZZ sex chromosomes. However, in early diverging plants and most macroalgae, sex is determined by female (U) or male (V) sex chromosomes in a haploid phase called the gametophyte. Once the U and V chromosomes unite at fertilization to produce a diploid sporophyte, sex determination no longer occurs, raising key questions about the fate of the U and V sex chromosomes in the sporophyte phase. Here, we investigate genetic and molecular interactions of the UV sex chromosomes in both the haploid and diploid phases of the brown alga Ectocarpus. We reveal extensive developmental regulation of sex chromosome genes across its life cycle and implicate the TALE-HD transcription factor OUROBOROS in suppressing sex determination in the diploid phase. Small RNAs may also play a role in the repression of a female sex-linked gene, and transition to the diploid sporophyte coincides with major reconfiguration of histone H3K79me2, suggesting a more intricate role for this histone mark in Ectocarpus development than previously appreciated.","PeriodicalId":11375,"journal":{"name":"Development","volume":null,"pages":null},"PeriodicalIF":4.6,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11057875/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140184036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Variations in cell plasticity and proliferation underlie distinct modes of regeneration along the antero-posterior axis in the annelid Platynereis. 细胞可塑性和增殖的变化是环带蝶类（Platynereis）沿前后轴再生的不同模式的基础。

IF 3.7 2区生物学

Development Pub Date : 2024-10-15 Epub Date: 2024-07-02 DOI: 10.1242/dev.202452

Loïc Bideau, Zoé Velasquillo-Ramirez, Loeiza Baduel, Marianne Basso, Pascale Gilardi-Hebenstreit, Vanessa Ribes, Michel Vervoort, Eve Gazave

{"title":"Variations in cell plasticity and proliferation underlie distinct modes of regeneration along the antero-posterior axis in the annelid Platynereis.","authors":"Loïc Bideau, Zoé Velasquillo-Ramirez, Loeiza Baduel, Marianne Basso, Pascale Gilardi-Hebenstreit, Vanessa Ribes, Michel Vervoort, Eve Gazave","doi":"10.1242/dev.202452","DOIUrl":"https://doi.org/10.1242/dev.202452","url":null,"abstract":"The capacity to regenerate lost tissues varies significantly among animals. Some phyla, such as the annelids, display substantial regenerating abilities, although little is known about the cellular mechanisms underlying the process. To precisely determine the origin, plasticity and fate of the cells participating in blastema formation and posterior end regeneration after amputation in the annelid Platynereis dumerilii, we developed specific tools to track different cell populations. Using these tools, we find that regeneration is partly promoted by a population of proliferative gut cells whose regenerative potential varies as a function of their position along the antero-posterior axis of the worm. Gut progenitors from anterior differentiated tissues are lineage restricted, whereas gut progenitors from the less differentiated and more proliferative posterior tissues are much more plastic. However, they are unable to regenerate the stem cells responsible for the growth of the worms. Those stem cells are of local origin, deriving from the cells present in the segment abutting the amputation plane, as are most of the blastema cells. Our results favour a hybrid and flexible cellular model for posterior regeneration in Platynereis relying on different degrees of cell plasticity.","PeriodicalId":11375,"journal":{"name":"Development","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141476187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phylogenomic analysis of the Lake Kronotskoe species flock of Dolly Varden charr reveals genetic and developmental signatures of sympatric radiation. 对克罗诺茨科湖多利瓦登鲑物种群的系统发生组分析揭示了同域辐射的遗传和发育特征。

IF 3.7 2区生物学

Development Pub Date : 2024-10-15 Epub Date: 2024-10-29 DOI: 10.1242/dev.203002

Katherine C Woronowicz, Evgeny V Esin, Grigorii N Markevich, Crisvely Soto Martinez, Sarah K McMenamin, Jacob M Daane, Matthew P Harris, Fedor N Shkil

{"title":"Phylogenomic analysis of the Lake Kronotskoe species flock of Dolly Varden charr reveals genetic and developmental signatures of sympatric radiation.","authors":"Katherine C Woronowicz, Evgeny V Esin, Grigorii N Markevich, Crisvely Soto Martinez, Sarah K McMenamin, Jacob M Daane, Matthew P Harris, Fedor N Shkil","doi":"10.1242/dev.203002","DOIUrl":"10.1242/dev.203002","url":null,"abstract":"Recent adaptive radiations provide experimental opportunities to parse the relationship between genomic variation and the origins of distinct phenotypes. Sympatric radiations of the charr complex (genus Salvelinus) present a trove for phylogenetic analyses as charrs have repeatedly diversified into multiple morphs with distinct feeding specializations. However, charr species flocks normally comprise only two to three lineages. Dolly Varden charr inhabiting Lake Kronotskoe represent the most extensive radiation described for the genus, containing at least seven lineages, each with defining morphological and ecological traits. Here, we perform the first genome-wide analysis of this species flock to parse the foundations of adaptive change. Our data support distinct, reproductively isolated lineages within the clade. We find that changes in genes associated with thyroid signaling and craniofacial development provided a foundational shift in evolution to the lake. The thyroid axis is further implicated in subsequent lineage partitioning events. These results delineate a genetic scenario for the diversification of specialized lineages and highlight a common axis of change biasing the generation of specific forms during adaptive radiation.","PeriodicalId":11375,"journal":{"name":"Development","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142460556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0