Development最新文献_第6页

Actin and myosin dynamics during epithelial remodeling in avian gastrulation. 肌动蛋白和肌球蛋白动力学在禽原肠形成上皮重塑过程中。

IF 4 2区生物学

Development Pub Date : 2026-09-15 Epub Date: 2026-09-28 DOI: 10.1242/dev.205461

Yu Ieda, Carole Phan, Olinda Alegria-Prévot, Aurélien Villedieu, Jérôme Gros

{"title":"Actin and myosin dynamics during epithelial remodeling in avian gastrulation.","authors":"Yu Ieda, Carole Phan, Olinda Alegria-Prévot, Aurélien Villedieu, Jérôme Gros","doi":"10.1242/dev.205461","DOIUrl":"10.1242/dev.205461","url":null,"abstract":"Epithelial remodeling is powered by contractile forces exerted by the actomyosin cytoskeleton. In invertebrates, pulsatile contractile flows of the medio-apical actomyosin cortex have been shown to be crucial in promoting junction contraction, ultimately driving cell rearrangements, apical constriction and cell extrusion. However, how actomyosin dynamics drive epithelial remodeling in vertebrates, especially amniotes, remains poorly understood. In this study, we generated transgenic quail lines reporting actin and myosin, and investigated their dynamics in gastrulating embryos. We show that, during this process, epithelial remodeling events are closely associated with the contraction of junctional myosin. Although we observe medio-apical contractile flows, these are irregular, produce only transient or subtle cell and junctional deformations, and are not consistently associated with junctional or cellular contraction during convergent extension of the primitive streak or during mesendodermal cell ingression. Notably, by characterizing live and apoptotic cell extrusions and their associated actomyosin dynamics, we provide new insights into the cellular processes underlying primitive streak formation and the emergence of germ layers.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148900150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pathway to Independence - an interview with Margherita Zamboni. 通往独立之路-对玛格丽塔·赞博尼的采访。

IF 4 2区生物学

Development Pub Date : 2026-09-15 Epub Date: 2026-09-21 DOI: 10.1242/dev.206087

引用次数: 0

Dual origins for neural cells during development of the Clytia planula larva. 扁平鱼幼虫发育过程中神经细胞的双重起源。

IF 4 2区生物学

Development Pub Date : 2026-09-15 Epub Date: 2026-09-18 DOI: 10.1242/dev.205645

Antonella Ruggiero, Anna Ferraioli, Sandra Chevalier, Pascal Lapébie, Romain Girard, Richard R Copley, Tsuyoshi Momose, Carine Barreau, Evelyn Houliston

{"title":"Dual origins for neural cells during development of the Clytia planula larva.","authors":"Antonella Ruggiero, Anna Ferraioli, Sandra Chevalier, Pascal Lapébie, Romain Girard, Richard R Copley, Tsuyoshi Momose, Carine Barreau, Evelyn Houliston","doi":"10.1242/dev.205645","DOIUrl":"https://doi.org/10.1242/dev.205645","url":null,"abstract":"Adult hydrozoan cnidarians undergo extensive tissue turnover, generating neural cell types that include nematocytes (stinging cells) and gland cells from interstitial stem cells (i-cells) expressing stemness proteins such as Piwi and Nanos. The contribution of i-cells during embryogenesis, however, has been unclear. We address neural cell origins during development of the Clytia hemisphaerica planula larva. In situ hybridisation of nematogenesis genes revealed that Piwi/Nanos1-expressing cells generate a substantial pool of nematoblasts (undifferentiated nematocytes) within the gastrula presumptive endoderm, in line with scRNAseq clustering analysis. Some neurogenic and neuronal markers, however, showed a distinct expression profile, developing within a basal layer of the aboral/lateral ectoderm during gastrulation. Embryo bisection and lineage-tracing experiments support the hypothesis that neurosensory and secretory cell types can be generated from gastrula ectoderm, while nematoblasts and at least some RFamide-expressing ganglionic cells are derived from i-cells. Knockdown and inhibitor treatments revealed steps in neuron and nematocyte development regulated by Wnt-β-catenin. We propose that two distinct neurogenesis pathways operate during Clytia embryogenesis: one involving ectodermal delamination, and one generating many nematoblasts and some neurons from i-cell-like precursors.","PeriodicalId":11375,"journal":{"name":"Development","volume":"153 18","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148988768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The people behind the papers - Chiyoko Machida and Ayami Nakagawa. 报纸背后的人——町田千代子和中川绫美。

IF 4 2区生物学

Development Pub Date : 2026-09-15 Epub Date: 2026-09-18 DOI: 10.1242/dev.206089

引用次数: 0

A retinoic acid-Rfx6 cascade modulates Wnt/Bmp-dependent gut tube patterning: insight into the molecular basis of Mitchell-Riley Syndrome. 视黄酸- rfx6级联调节Wnt/ bmp依赖性肠管模式：洞见米切尔-莱利综合征的分子基础

IF 4 2区生物学

Development Pub Date : 2026-09-15 Epub Date: 2026-09-22 DOI: 10.1242/dev.205552

Scott A Rankin, Jacek Biesiada, Kyle W McCracken, Riya K Patel, Mehek Rana, David M Luedeke, Daniel O Kechele, James M Wells, Aaron M Zorn

{"title":"A retinoic acid-Rfx6 cascade modulates Wnt/Bmp-dependent gut tube patterning: insight into the molecular basis of Mitchell-Riley Syndrome.","authors":"Scott A Rankin, Jacek Biesiada, Kyle W McCracken, Riya K Patel, Mehek Rana, David M Luedeke, Daniel O Kechele, James M Wells, Aaron M Zorn","doi":"10.1242/dev.205552","DOIUrl":"10.1242/dev.205552","url":null,"abstract":"Multiple signaling pathways and transcription factors (TFs) establish organ domains in the developing gastrointestinal tract. How these are integrated into spatial-temporal networks to regulate organogenesis and how disruptions to those networks lead to congenital syndromes remain poorly understood. Using human pluripotent stem cell cultures and Xenopus embryos, we demonstrate that retinoic acid (RA) from the lateral plate mesoderm directly activates expression of the TF rfx6 in posterior foregut endoderm. Rfx6 subsequently promotes posterior foregut identity while suppressing Wnt-dependent hindgut and Bmp-dependent pharyngeal fates through direct and indirect mechanisms. Rfx6 can directly activate the expression of several key foregut TFs (onecut1 and pdx1) and Wnt antagonists (sfrp2/5) while indirectly restricting expression of Wnt and Bmp ligands. Rfx6 also directly suppresses transcription of the Wnt-dependent hindgut TF cdx2 and the Bmp-dependent pharyngeal TFs nkx2-5 and nkx2-6. Thus, Rfx6 acts at multiple levels to integrate RA, Wnt and Bmp activity into a network with lineage-promoting TFs to control gut tube patterning. These results provide insight into the molecular basis of Mitchell-Riley Syndrome congenital anomalies, which are caused by RFX6 mutations.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13677009/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148864152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Arabidopsis epigenetic factor AS2 mitigates nucleolar stress and promotes flat leaf development by repressing a CDK inhibitor. 拟南芥表观遗传因子AS2通过抑制CDK抑制剂减轻核核胁迫并促进平叶发育。

IF 4 2区生物学

Development Pub Date : 2026-09-15 Epub Date: 2026-09-18 DOI: 10.1242/dev.205275

Ayami Nakagawa, Hidekazu Iwakawa, Hiro Takahashi, Simon Vial-Pradel, Mari Takahashi, Kazuomi Ohga, Takuma Ito, Motoki Tamai, Eri Takada, Sumie Keta, Nanako Ishibashi, Sayuri Ando, Iwai Ohbayashi, Masaki Ito, Mami Yamazaki, Ayato Sato, Masaaki Umeda, Byung-Yoon Cha, Je-Tae Woo, Michiko Sasabe, Munetaka Sugiyama, Shoko Kojima, Yasunori Machida, Chiyoko Machida

{"title":"Arabidopsis epigenetic factor AS2 mitigates nucleolar stress and promotes flat leaf development by repressing a CDK inhibitor.","authors":"Ayami Nakagawa, Hidekazu Iwakawa, Hiro Takahashi, Simon Vial-Pradel, Mari Takahashi, Kazuomi Ohga, Takuma Ito, Motoki Tamai, Eri Takada, Sumie Keta, Nanako Ishibashi, Sayuri Ando, Iwai Ohbayashi, Masaki Ito, Mami Yamazaki, Ayato Sato, Masaaki Umeda, Byung-Yoon Cha, Je-Tae Woo, Michiko Sasabe, Munetaka Sugiyama, Shoko Kojima, Yasunori Machida, Chiyoko Machida","doi":"10.1242/dev.205275","DOIUrl":"10.1242/dev.205275","url":null,"abstract":"The Arabidopsis thaliana leaf, which exhibits a symmetrically extended flat morphology, consists of two distinct cellular domains: adaxial and abaxial layers. The ASYMMETRIC LEAVES2 (AS2) gene is essential for specifying the adaxial domain, and its protein forms nucleolar structures, termed AS2 bodies, at ribosomal-DNA loci. Numerous nucleolus-related genes have been reported to be cooperatively involved in leaf adaxialization together with AS2. However, the molecular relationships between AS2 and these genes remain unclear. To identify chemical modulators of AS2 function, we screened a chemical library containing natural products and identified eight molecules, including camptothecin, that induced filamentous leaves in the as2 mutant. Camptothecin is an inhibitor of topoisomerase I, which is required for transcription of ribosomal RNA genes, and induces nucleolar stress. Treatment with 10-hydroxy-camptothecin increased transcript levels of the CDK inhibitor KIP-RELATED-PROTEIN5/INHIBITOR-OF-CYCLIN-DEPENDENT-KINASE3 (KRP5/ICK3), and caused notable changes in the number and morphology of AS2 bodies. Given its role in leaf morphogenesis, our findings suggest that AS2 is a key factor in leaf adaxialization, which leads to the formation of flat leaves, and in protection against nucleolar stress through coordination with nucleolar proteins.","PeriodicalId":11375,"journal":{"name":"Development","volume":"153 18","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13680833/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148988838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The people behind the papers - Carlos Carmocho-Macorra and Benjamin Steventon. 报纸背后的人，卡洛斯·卡莫乔·马科拉和本杰明·史蒂文顿。

IF 4 2区生物学

Development Pub Date : 2026-09-01 Epub Date: 2026-09-11 DOI: 10.1242/dev.206084

引用次数: 0

Coupling Vgll4b/Yap-regulated posterior cell addition with anterior vacuolation enables robust notochord elongation. Vgll4b/Yap调节后细胞增加与前空泡化的耦合使脊索伸长稳健。

IF 4 2区生物学

Development Pub Date : 2026-09-01 Epub Date: 2026-09-11 DOI: 10.1242/dev.205951

Carlos Camacho-Macorra, Alberto S Ceccarelli, Guillermo Serrano Nájera, Dillan Saunders, Isabella Boesgaard, Osvaldo Chara, Benjamin Steventon

{"title":"Coupling Vgll4b/Yap-regulated posterior cell addition with anterior vacuolation enables robust notochord elongation.","authors":"Carlos Camacho-Macorra, Alberto S Ceccarelli, Guillermo Serrano Nájera, Dillan Saunders, Isabella Boesgaard, Osvaldo Chara, Benjamin Steventon","doi":"10.1242/dev.205951","DOIUrl":"10.1242/dev.205951","url":null,"abstract":"Robust tissue growth control requires long-range communication between the rate of progenitor addition and tissue expansion. However, the regulatory mechanisms that couple these processes are unknown. In zebrafish, notochord morphogenesis is a driver of axis extension through both posterior progenitor addition and anterior vacuolation. To elucidate how progenitor dynamics and vacuole-driven cell expansion interact to elongate the notochord, we generated a mathematical model linking progenitor addition rate to the expansion of cells from anterior to posterior to simulate vacuolation rate. Comparing this with empirical measurements, we find that progenitor incorporation, together with vacuolation, produces a linear gradient in nearest neighbour distance. We next explored the role of YAP/TAZ in regulating progenitor addition in mutants for the YAP/TAZ inhibitor vgll4b. We find that vgll4b expression and YAP activity are enriched in posterior midline progenitors. Loss of vgll4b elevates YAP signalling, enhances progenitor addition, restricts vacuole expansion, and - after a transient buffering phase - compromises anterior-posterior axis elongation. These results support a long-range feedback mechanism linking progenitor recruitment to vacuolation, enabling the notochord to balance cellular input with volumetric expansion, thereby maintaining tissue proportions.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13615686/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148758222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immune regulation of neuronal stem cells in the medaka retina. medaka视网膜中神经元干细胞的免疫调节。

IF 4 2区生物学

Development Pub Date : 2026-09-01 Epub Date: 2026-09-10 DOI: 10.1242/dev.205198

Rashi Agarwal, Joergen Benjaminsen, Katharina Lust, Clara Julseth, Natalia Fuchs, Eva Hasel de Carvalho, Fanny Eggeler, Omnia El Said Ibrahim, Narges Aghaallaei, Baubak Bajoghli, Joachim Wittbrodt

{"title":"Immune regulation of neuronal stem cells in the medaka retina.","authors":"Rashi Agarwal, Joergen Benjaminsen, Katharina Lust, Clara Julseth, Natalia Fuchs, Eva Hasel de Carvalho, Fanny Eggeler, Omnia El Said Ibrahim, Narges Aghaallaei, Baubak Bajoghli, Joachim Wittbrodt","doi":"10.1242/dev.205198","DOIUrl":"10.1242/dev.205198","url":null,"abstract":"Stem cell populations in tissues require precise regulation of their number and quality to maintain proper organ growth. Among the various regulatory mechanisms, immune cells are emerging to directly regulate stem cell populations. The medaka retinal stem cell (RSC) niche, a model for lifelong neurogenic growth, provides a system for studying immune-stem cell interactions. We investigate how microglia, which are resident macrophages of the central nervous system, regulate the RSC niche. We identify that bona fide RSCs express the chemokine Ccl25b, while its cognate receptor, Ccr9a, is expressed in microglia. These microglia form a surveillance ring adjacent to the RSC niche and actively phagocytose RSCs. Interference with microglia by deletion of spi1b reveals that microglia absence leads to increased numbers of ccl25b-positive RSCs and results in morphological defects of the retina. Targeted mutation of ccl25b specifically affects microglia mobility under injury conditions; however, we did not observe any morphological defects, indicating that Ccl25b-Ccr9a signaling is not essential for stem cell maintenance. Overall, our data show that, under homeostatic conditions, the individual RSCs, which are essential for proper eye development, are actively phagocytosed by immune surveillance.","PeriodicalId":11375,"journal":{"name":"Development","volume":"153 17","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13615679/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148917872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

HDAC1/2-mediated repression of Wnt receptor expression orients asymmetric division polarity in Caenorhabditis elegans. hdac1 /2介导的Wnt受体表达抑制定向线虫不对称分裂极性。

IF 4 2区生物学

Development Pub Date : 2026-09-01 Epub Date: 2026-08-28 DOI: 10.1242/dev.205658

Mar Ferrando-Marco, Shuxiao Lin, Beatriz Garcia Del Valle, Mark Hintze, Lucy Narunsky, Junyue Huang, Shannon Edwards, Michalis Barkoulas

{"title":"HDAC1/2-mediated repression of Wnt receptor expression orients asymmetric division polarity in Caenorhabditis elegans.","authors":"Mar Ferrando-Marco, Shuxiao Lin, Beatriz Garcia Del Valle, Mark Hintze, Lucy Narunsky, Junyue Huang, Shannon Edwards, Michalis Barkoulas","doi":"10.1242/dev.205658","DOIUrl":"10.1242/dev.205658","url":null,"abstract":"Asymmetric cell division generates distinct daughter cells essential for tissue development, yet the mechanisms orienting division polarity within tissues remain incompletely understood. Here, we uncover a role for chromatin-mediated transcriptional repression in controlling polarity orientation during asymmetric division of Caenorhabditis elegans epidermal stem cells, known as the seam cells. Tissue-specific loss of the class I histone deacetylase hda-1, homologous to mammalian HDAC1/2, causes polarity reversals and reduces molecular asymmetry between daughter cells. Using Targeted DamID, we identify the Wnt receptors lin-17 (frizzled homologue) and cam-1 (Ror homologue) as key targets. Single-molecule fluorescence in situ hybridisation reveals opposing expression gradients along the body axis, with cam-1 enriched anteriorly and lin-17 posteriorly. In hda-1 mutants, both receptors are upregulated and differences between seam cells are flattened. Overexpression of either receptor alone is sufficient to reproduce the polarity reversals, while co-overexpression produces additive effects. The polarity phenotype is independent of the canonical NuRD and SIN3 complexes, suggesting that HDA-1 acts through an alternative mechanism. These findings link histone deacetylase activity to Wnt receptor expression and suggest that graded receptor expression may provide cues that orient asymmetric division polarity.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13615694/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148663696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0