Development最新文献_第6页

The people behind the papers - Dhaval Gandhi and Jessica Treisman. 这些报纸背后的人，哈瓦尔·甘地和杰西卡·特雷斯曼。

IF 3.6 2区生物学

Development Pub Date : 2025-09-15 Epub Date: 2025-09-18 DOI: 10.1242/dev.205212

引用次数: 0

The people behind the papers - Judith Pineau, Jerome Wong-Ng and Thomas Gregor. 这些报纸背后的人——朱迪思·皮诺、杰罗姆·黄和托马斯·格雷戈尔。

IF 3.6 2区生物学

Development Pub Date : 2025-09-15 Epub Date: 2025-09-29 DOI: 10.1242/dev.205228

引用次数: 0

Nascent dendrite branches initiated by a localized burst of Spire-dependent actin polymerization. 新生的树突分支是由依赖于尖的肌动蛋白聚合的局部爆发引发的。

IF 3.6 2区生物学

Development Pub Date : 2025-09-15 Epub Date: 2025-09-25 DOI: 10.1242/dev.204786

Deirdre Hatton, Claire Marquilly, Caitlin Hanrahan, Tiago Ferreira, Yimiao Ou, Lauren Cinq-Mars, Will Silkworth, Hannah M Bailey, Margot E Quinlan, Donald J van Meyel

{"title":"Nascent dendrite branches initiated by a localized burst of Spire-dependent actin polymerization.","authors":"Deirdre Hatton, Claire Marquilly, Caitlin Hanrahan, Tiago Ferreira, Yimiao Ou, Lauren Cinq-Mars, Will Silkworth, Hannah M Bailey, Margot E Quinlan, Donald J van Meyel","doi":"10.1242/dev.204786","DOIUrl":"10.1242/dev.204786","url":null,"abstract":"Dendrites form arbors whose size, shape and complexity define how neurons cover their receptive territories. Actin dynamics contribute to growth and remodeling of dendrite arbors. Here, we have examined how Spire, a conserved actin nucleation factor, promotes the formation of new branches in vivo. In live imaging of Drosophila class IV dendritic arborization (c4da) neurons, Spire was observed at new sites of branch initiation, where it assembled new actin polymer in a burst immediately before filopodial outgrowth. For dendrite arborization, Spire required intact structural domains to nucleate actin and target the secretory network, and interacted with Rab11 GTPase, a key regulator of recycling endosomes. Together, these findings support a model in which Spire cooperates with Rab11 to promote new dendrite branches by linking localized actin dynamics with intracellular trafficking of endosomes that deliver lipids and cargoes to fuel protrusive outgrowth of nascent dendrites.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12516318/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145039388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In preprints: gastruloids get (a)head. 在预印本中：类胃原体得到(a)头部。

IF 3.6 2区生物学

Development Pub Date : 2025-09-15 DOI: 10.1242/dev.205199

Wilhelmus A Brouwer, Luca Braccioli

引用次数: 0

Fine-tuning mechanical constraints reveals uncoupled patterning and gene expression programs in murine gastruloids. 微调机械约束揭示了小鼠类胃原体中不耦合的模式和基因表达程序。

IF 3.6 2区生物学

Development Pub Date : 2025-09-15 Epub Date: 2025-09-29 DOI: 10.1242/dev.204711

Judith Pineau, Jerome Wong-Ng, Alexandre Mayran, Lucille Lopez-Delisle, Pierre Osteil, Armin Shoushtarizadeh, Denis Duboule, Samy Gobaa, Thomas Gregor

{"title":"Fine-tuning mechanical constraints reveals uncoupled patterning and gene expression programs in murine gastruloids.","authors":"Judith Pineau, Jerome Wong-Ng, Alexandre Mayran, Lucille Lopez-Delisle, Pierre Osteil, Armin Shoushtarizadeh, Denis Duboule, Samy Gobaa, Thomas Gregor","doi":"10.1242/dev.204711","DOIUrl":"10.1242/dev.204711","url":null,"abstract":"The interplay between mechanical forces and genetic programs is fundamental to embryonic development, yet how these factors influence morphogenesis and cell fate decisions remains unclear. Here, we fine-tune the mechanical environment of murine gastruloids, three-dimensional in vitro models of early embryogenesis, by embedding them in bioinert hydrogels with tunable stiffness and timing. This strategy reveals that external constraints can selectively influence transcriptional profiles, patterning or morphology, depending on the level and timing of mechanical modulation. Gastruloids in ultra-soft hydrogels (<30 Pa) elongate robustly, preserving anteroposterior patterning and transcriptional profiles. In contrast, embedding at higher stiffness disrupts polarization while leaving gene expression largely unaffected. Conversely, earlier embedding significantly impacts transcriptional profiles independently of polarization defects, highlighting the uncoupling of patterning and transcription. These findings suggest that distinct cellular states respond differently to external constraints. Live imaging and cell tracking further suggest that impaired cell motility underlies polarization defects, underscoring the role of mechanical forces in shaping morphogenesis independently of transcriptional changes. By precisely controlling mechanical boundaries, our approach provides a powerful platform to dissect how physical and biochemical factors interact to orchestrate embryonic development.","PeriodicalId":11375,"journal":{"name":"Development","volume":"152 18","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12516319/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145184928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

FGF diffusion is required for directed migration of postembryonic muscle progenitors in C. elegans. 秀丽隐杆线虫胚胎后肌肉祖细胞的定向迁移需要FGF的扩散。

IF 3.6 2区生物学

Development Pub Date : 2025-09-15 Epub Date: 2025-09-22 DOI: 10.1242/dev.204802

Theresa V Gibney, Ariel M Pani

{"title":"FGF diffusion is required for directed migration of postembryonic muscle progenitors in C. elegans.","authors":"Theresa V Gibney, Ariel M Pani","doi":"10.1242/dev.204802","DOIUrl":"10.1242/dev.204802","url":null,"abstract":"Extracellular signaling molecules mediate crucial aspects of cell-cell communication and play essential roles in development and homeostasis. Fibroblast growth factors (FGFs) are a family of secreted signaling proteins that can disperse long distances between cells and are often thought to form concentration gradients that encode spatial information. However, we know relatively little about the spatial distribution of FGFs in vivo, and endogenously tagged FGFs move between cells using different mechanisms in zebrafish and flies. We used FGF-dependent migration of C. elegans muscle progenitors called sex myoblasts (SMs) to elucidate FGF dispersal mechanisms and dissect how FGF guides migrating cells. Live imaging of cell dynamics and endogenously tagged FGF combined with membrane tethering and extracellular trapping approaches revealed that endogenous FGF is diffusible in vivo and extracellular dispersal is required for SM migration. Misexpression demonstrated that FGF is a bona fide chemoattractant that orients SMs during a critical window, while an unidentified, short-range signal acts in concert to position SMs precisely. Our finding that an invertebrate FGF is endogenously diffusible suggests that this may be the ancestral mode for FGF dispersal.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12516322/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144947029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From stem cells to human development: supporting a growing field. 从干细胞到人类发展：支持一个不断发展的领域。

IF 3.6 2区生物学

Development Pub Date : 2025-09-15 DOI: 10.1242/dev.205181

Katherine Brown

引用次数: 0

Circadian clock genes perform dual roles as systemic oscillators and context-dependent regulators. 昼夜节律钟基因作为系统振荡器和环境依赖的调节器发挥双重作用。

IF 3.6 2区生物学

Development Pub Date : 2025-09-15 Epub Date: 2025-09-29 DOI: 10.1242/dev.204841

Nozomu Takahashi, Motomu Endo

引用次数: 0

The non-canonical thioreductase Tmx2b is essential for neuronal survival during zebrafish embryonic brain development. 非规范硫还原酶Tmx2b对斑马鱼胚胎脑发育过程中的神经元存活至关重要。

IF 3.6 2区生物学

Development Pub Date : 2025-09-15 Epub Date: 2025-09-18 DOI: 10.1242/dev.204348

Jordy Dekker, Wendy Lam, Herma C van der Linde, Floris Ophorst, Charlotte de Konink, Rachel Schot, Gert-Jan Kremers, Leslie E Sanderson, Woutje M Berdowski, Geeske M van Woerden, Grazia M S Mancini, Tjakko J van Ham

{"title":"The non-canonical thioreductase Tmx2b is essential for neuronal survival during zebrafish embryonic brain development.","authors":"Jordy Dekker, Wendy Lam, Herma C van der Linde, Floris Ophorst, Charlotte de Konink, Rachel Schot, Gert-Jan Kremers, Leslie E Sanderson, Woutje M Berdowski, Geeske M van Woerden, Grazia M S Mancini, Tjakko J van Ham","doi":"10.1242/dev.204348","DOIUrl":"10.1242/dev.204348","url":null,"abstract":"Biallelic variants in thioredoxin-related transmembrane 2 protein (TMX2) can cause a malformation of brain cortical development characterized by microcephaly, polymicrogyria and pachygyria by an unknown mechanism. To investigate and visualize how TMX2 loss disrupts brain development in vivo, we generated zebrafish deficient for TMX2 ortholog tmx2b, which during the first two developmental days showed normal brain developmental hallmarks. From 3 days onwards, however, tmx2b mutants had no locomotor activity; this was accompanied by cell death in the brain, but not in other organs or in the spinal cord. Strikingly, cell death in tmx2b mutants occurred specifically in post-mitotic neurons within a ∼1.5-h timeframe, whereas neuronal progenitor and radial glial cells were preserved, and could be suppressed by inhibiting neuronal activity. In vivo calcium imaging showed a persistent ∼2-fold increase in calcium in neurons after the onset of cell death. This suggests that calcium homeostasis underlies the tmx2b mutant brain phenotype. Our results indicate that TMX2 is an evolutionarily conserved, protective regulator essential specifically for post-mitotic neurons after their differentiation in the vertebrate embryonic brain.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12516324/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144947040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transitions in Development - an interview with Mijo Šimunović. 开发中的过渡-对Mijo的采访Šimunović。

IF 3.6 2区生物学

Development Pub Date : 2025-09-15 DOI: 10.1242/dev.205185

引用次数: 0