Development最新文献_第5页

Distinct effects of CDK8 module subunits on cellular growth and proliferation in Drosophila. CDK8 模块亚基对果蝇细胞生长和增殖的不同影响

IF 3.7 2区生物学

Development Pub Date : 2024-12-01 Epub Date: 2024-11-29 DOI: 10.1242/dev.203111

Xiao Li, Mengmeng Liu, Yue Xing, Ye Niu, Tzu-Hao Liu, Jasmine L Sun, Yanwu Liu, Rajitha-Udakara-Sampath Hemba-Waduge, Jun-Yuan Ji

{"title":"Distinct effects of CDK8 module subunits on cellular growth and proliferation in Drosophila.","authors":"Xiao Li, Mengmeng Liu, Yue Xing, Ye Niu, Tzu-Hao Liu, Jasmine L Sun, Yanwu Liu, Rajitha-Udakara-Sampath Hemba-Waduge, Jun-Yuan Ji","doi":"10.1242/dev.203111","DOIUrl":"10.1242/dev.203111","url":null,"abstract":"The Mediator complex plays a pivotal role in facilitating RNA polymerase II-dependent transcription in eukaryotes. Within this complex, the CDK8 kinase module (CKM), comprising CDK8, Cyclin C (CycC), Med12 and Med13, serves as a dissociable subcomplex that modulates the activity of the small Mediator complex. Genetic studies in Drosophila have revealed distinct phenotypes associated with mutations in CKM subunits, but the underlying mechanisms have remained unclear. Using Drosophila as a model, we generated transgenic strains to deplete individually or simultaneously the four CKM subunits in all possible combinations, uncovering unique phenotypes in the eyes and wings. Depletion of CDK8-CycC enhanced E2F1 target gene expression and promoted cell-cycle progression, whereas Med12-Med13 depletion had no significant impact on these processes. Instead, depleting Med12-Med13 altered the expression of ribosomal protein genes and fibrillarin, and reduced nascent protein synthesis, indicating a severe reduction in ribosome biogenesis and cellular growth compared to the loss of CDK8-CycC. These findings reveal distinct in vivo roles for CKM subunits, with Med12-Med13 disruption having a more pronounced effect on ribosome biogenesis and protein synthesis than CDK8-CycC loss.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11634032/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142616845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transitions in development - an interview with Kara McKinley.

IF 3.7 2区生物学

Development Pub Date : 2024-12-01 Epub Date: 2024-11-29 DOI: 10.1242/dev.204456

引用次数: 0

ARPP19 phosphorylation site evolution and the switch in cAMP control of oocyte maturation in vertebrates. ARPP19磷酸化位点进化与脊椎动物卵母细胞成熟的 cAMP 控制转换

IF 3.7 2区生物学

Development Pub Date : 2024-12-01 Epub Date: 2024-12-05 DOI: 10.1242/dev.202655

Ferdinand Meneau, Pascal Lapébie, Enrico Maria Daldello, Tran Le, Sandra Chevalier, Sarah Assaf, Evelyn Houliston, Catherine Jessus, Marika Miot

{"title":"ARPP19 phosphorylation site evolution and the switch in cAMP control of oocyte maturation in vertebrates.","authors":"Ferdinand Meneau, Pascal Lapébie, Enrico Maria Daldello, Tran Le, Sandra Chevalier, Sarah Assaf, Evelyn Houliston, Catherine Jessus, Marika Miot","doi":"10.1242/dev.202655","DOIUrl":"10.1242/dev.202655","url":null,"abstract":"cAMP-PKA signaling initiates the crucial process of oocyte meiotic maturation in many animals, but inhibits it in vertebrates. To address this 'cAMP paradox', we exchanged the key PKA substrate ARPP19 between representative species, the vertebrate Xenopus and the cnidarian Clytia, comparing its phosphorylation and function. We found that, as in Xenopus, Clytia maturing oocytes undergo ARPP19 phosphorylation on a highly conserved Gwl site, which inhibits PP2A and promotes M-phase entry. In contrast, despite a PKA phosphorylation signature motif recognizable across most animals, Clytia ARPP19 was only poorly phosphorylated by PKA in vitro and in vivo. Furthermore, unlike Xenopus ARPP19, exogenous Clytia ARPP19 did not delay Xenopus oocyte maturation. We conclude that, in Clytia, ARPP19 does not intervene in oocyte maturation initiation because of both poor recognition by PKA and the absence of effectors that mediate vertebrate oocyte prophase arrest. We propose that ancestral ARPP19 phosphorylated by Gwl has retained a key role in M-phase across eukaryotes and has acquired new functions during animal evolution mediated by enhanced PKA phosphorylation, allowing co-option into oocyte maturation regulation in the vertebrate lineage.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142686086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gonadal sex reversal at single-cell resolution in Znrf3-deficient mice.

IF 3.7 2区生物学

Development Pub Date : 2024-12-01 Epub Date: 2024-12-04 DOI: 10.1242/dev.202707

Raissa G G Kay, Richard Reeves, Pam Siggers, Simon Greenaway, Ann-Marie Mallon, Sara Wells, Bon-Kyoung Koo, Chloé Mayère, Serge Nef, Andy Greenfield, Michelle M Simon

{"title":"Gonadal sex reversal at single-cell resolution in Znrf3-deficient mice.","authors":"Raissa G G Kay, Richard Reeves, Pam Siggers, Simon Greenaway, Ann-Marie Mallon, Sara Wells, Bon-Kyoung Koo, Chloé Mayère, Serge Nef, Andy Greenfield, Michelle M Simon","doi":"10.1242/dev.202707","DOIUrl":"10.1242/dev.202707","url":null,"abstract":"The role of anti-WNT ZNRF3 is central to determining gonadal fate: XY mice lacking functional ZNRF3 exhibit a highly variable gonadal sex reversal phenotype in the fetal period, characterised by appearance of ovarian tissue. To investigate this sex reversal further, we used single-cell RNA-seq to examine the transcriptomes of XY Znrf3-deficient gonads during the mouse sex-determining period. Analyses of cell trajectories in mutant gonads reveal the failure of pre-supporting cells to commit to the Sertoli cell fate, XY granulosa cell development, unstable commitment in those cells that reach the Sertoli path and enhanced contribution to a supporting-like cell fate. By developing a machine learning-based score for transcriptomic similarity to Sertoli and granulosa, we show pervasive disruption to acquisition of testicular cell fate in the mutant supporting cell lineage, with large numbers of cells co-expressing pro-Sertoli and pro-granulosa markers. These data reveal that loss of Znrf3 results in transcriptomic and cellular heterogeneity, with shifts in cellular sex identity that undermine a simple binary model in which mutant supporting cell precursors achieve either Sertoli or granulosa cell differentiation.","PeriodicalId":11375,"journal":{"name":"Development","volume":"151 23","pages":""},"PeriodicalIF":3.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11658683/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142767134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Contributions of the Dachsous intracellular domain to Dachsous-Fat signaling. Dachsous 细胞内结构域对 Dachsous-Fat 信号转导的贡献。

IF 3.7 2区生物学

Development Pub Date : 2024-12-01 Epub Date: 2024-11-29 DOI: 10.1242/dev.202919

Bipin Kumar Tripathi, Kenneth D Irvine

{"title":"Contributions of the Dachsous intracellular domain to Dachsous-Fat signaling.","authors":"Bipin Kumar Tripathi, Kenneth D Irvine","doi":"10.1242/dev.202919","DOIUrl":"10.1242/dev.202919","url":null,"abstract":"The protocadherins Fat and Dachsous regulate organ growth, shape, patterning, and planar cell polarity. Although Dachsous and Fat have been described as ligand and receptor, respectively, in a signal transduction pathway, there is also evidence for bidirectional signaling. Here, we assess signaling downstream of Dachsous through analysis of its intracellular domain. Genomic deletions of conserved sequences within dachsous identified regions of the intracellular domain that contribute to Dachsous activity. Deletion of the A motif increased Dachsous protein levels and decreased wing size. Deletion of the D motif decreased Dachsous levels at cell membranes, increased wing size, and disrupted wing, leg and hindgut patterning and planar cell polarity. Co-immunoprecipitation experiments established that the D motif is necessary and sufficient for association of Dachsous with key partners, including Lowfat, Dachs, Spiny-legs, Fat and MyoID. Subdivision of the D motif identified distinct regions that preferentially contribute to different Dachsous activities. Our results identify motifs that are essential for Dachsous function and are consistent with the hypothesis that the key function of Dachsous is regulation of Fat.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11634027/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142582270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Constructive Critics: Development's approach to peer review.

IF 3.7 2区生物学

Development Pub Date : 2024-12-01 Epub Date: 2024-11-29 DOI: 10.1242/dev.204523

James Briscoe, Katherine Brown

引用次数: 0

The m5C reader protein Ybx1 promotes axon growth by regulating local translation in axons.

IF 3.7 2区生物学

Development Pub Date : 2024-12-01 Epub Date: 2024-11-29 DOI: 10.1242/dev.202781

Jun Yu, Yanbo Zhang, Jian Zhang, Pengfei Che, Gaoxin Long, Zhuoxuan Yang, Sheng-Jian Ji

引用次数: 0

WNT7A-positive dendritic cytonemes control synaptogenesis in cortical neurons. Wnt7a阳性树突细胞核控制大脑皮层神经元的突触发生

IF 3.7 2区生物学

Development Pub Date : 2024-12-01 Epub Date: 2024-12-09 DOI: 10.1242/dev.202868

Thomas M Piers, Kevin Fang, Seema C Namboori, Corin Liddle, Sally Rogers, Akshay Bhinge, Richard Killick, Steffen Scholpp

{"title":"WNT7A-positive dendritic cytonemes control synaptogenesis in cortical neurons.","authors":"Thomas M Piers, Kevin Fang, Seema C Namboori, Corin Liddle, Sally Rogers, Akshay Bhinge, Richard Killick, Steffen Scholpp","doi":"10.1242/dev.202868","DOIUrl":"10.1242/dev.202868","url":null,"abstract":"Synaptogenesis involves the transformation of dendritic filopodial contacts into stable connections with the exact apposition of synaptic components. Signalling triggered by Wnt/β-catenin and calcium has been postulated to aid this process. However, it is unclear how such a signalling process orchestrates synapse formation to organise the spatial arrangement of synapses along dendrites. We show that WNT7A is loaded on dynamic dendritic filopodia during spine formation in human cortical neurons. WNT7A is present at the tips of the filopodia and the contact sites with dendrites of neighbouring neurons, triggering spatially restricted localisation of the Wnt co-receptor LRP6. Here, we demonstrate that WNT7A at filopodia tips leads to the induction of calcium transients, the clustering of pre- and postsynaptic proteins, and the subsequent transformation into mature spines. Although soluble WNT7A protein can also support synaptogenesis, it fails to provide this degree of spatial information for spine formation and calcium transients, and synaptic markers are induced ectopically along the dendrites. Our data suggest that dendritic filopodia are WNT7A-bearing cytonemes required for focal calcium signalling and initiation of synapse formation, and provide an elegant mechanism for orchestrating the positioning of synapses along dendrites.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11658684/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142686170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A timed epigenetic switch balances T and ILC lineage proportions in the thymus.

IF 3.7 2区生物学

Development Pub Date : 2024-12-01 Epub Date: 2024-12-10 DOI: 10.1242/dev.203016

Nicholas A Pease, Kathryn M Denecke, Lihua Chen, Peter Habib Gerges, Hao Yuan Kueh

{"title":"A timed epigenetic switch balances T and ILC lineage proportions in the thymus.","authors":"Nicholas A Pease, Kathryn M Denecke, Lihua Chen, Peter Habib Gerges, Hao Yuan Kueh","doi":"10.1242/dev.203016","DOIUrl":"10.1242/dev.203016","url":null,"abstract":"How multipotent progenitors give rise to multiple cell types in defined numbers is a central question in developmental biology. Epigenetic switches, acting at single gene loci, can generate extended delays in the activation of lineage-specifying genes and impact lineage decisions and cell type output. Here, we analyzed a timed epigenetic switch controlling expression of mouse Bcl11b, a transcription factor that drives T-cell commitment, but only after a multi-day delay. To investigate roles for this delay in controlling lineage decision making, we analyzed progenitors with a deletion in a distal Bcl11b enhancer, which extends this delay by ∼3 days. Strikingly, delaying Bcl11b activation reduces T-cell output but enhances innate lymphoid cell (ILC) generation in the thymus by redirecting uncommitted progenitors to the ILC lineages. Mechanistically, delaying Bcl11b activation promoted ILC redirection by enabling upregulation of the ILC-specifying transcription factor PLZF. Despite the upregulation of PLZF, committed ILC progenitors could subsequently express Bcl11b, which is also needed for type 2 ILC differentiation. These results show that epigenetic switches can control the activation timing and order of lineage-specifying genes to modulate cell type numbers and proportions.","PeriodicalId":11375,"journal":{"name":"Development","volume":"151 23","pages":""},"PeriodicalIF":3.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11664168/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142799758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Abrupt-mediated control of ninjurins regulates Drosophila sessile haemocyte compartments. 果蝇无柄血球细胞区系由九头蛇蛋白突然介导的控制调节。

IF 3.7 2区生物学

Development Pub Date : 2024-12-01 Epub Date: 2024-12-09 DOI: 10.1242/dev.202977

So Yeon Kwon, Kimberly Chan, Martin Stofanko, Ka Hei Chan, Paul Badenhorst

{"title":"Abrupt-mediated control of ninjurins regulates Drosophila sessile haemocyte compartments.","authors":"So Yeon Kwon, Kimberly Chan, Martin Stofanko, Ka Hei Chan, Paul Badenhorst","doi":"10.1242/dev.202977","DOIUrl":"10.1242/dev.202977","url":null,"abstract":"Macrophage-like cells called haemocytes are key effectors of Drosophila cellular innate immune function. Larval haemocytes exist either in circulation or localize to segmentally repeated sessile haemocyte compartments (SHCs). While numerous functions have been proposed for SHCs, the mechanisms directing haemocytes to them are unclear. Here, we have exploited the developmentally regulated dispersal of SHCs that occurs at pupariation to identify the Abrupt (Ab) transcription factor (TF) and ninjurin cell-adhesion molecules as regulators of haemocyte recruitment to SHCs. We show that larval haemocytes express ninjurins, which are required for targeting haemocytes to SHCs. However, at pupariation, ecdysteroid signalling stimulates Ab expression, which collaborates with TFs, including Blimp-1 and Hr3, to repress ninjurins and disperse haemocytes. We observe that experimental manipulations that antagonize ninjurin function in larval haemocytes cause premature SHC dispersal, while stabilization of ninjurins in haemocytes blocks developmentally regulated SHC remodelling and increases sensitivity to immune challenges. Cumulatively, our data indicate that control of ninjurin activity provides a common target through which diverse developmental, environmental and immune stimuli can be integrated to control haemocyte dispersal and immune function.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11664169/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142638275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0