Development最新文献_第5页

Destabilisation of bam transcripts terminates the mitotic phase of Drosophila female germline differentiation. bam转录本的不稳定终止了果蝇雌性种系分化的有丝分裂期。

IF 3.7 2区生物学

Development Pub Date : 2025-03-01 Epub Date: 2025-03-11 DOI: 10.1242/dev.204405

Tamsin J Samuels, Elizabeth J Torley, Valeriia Nadmitova, Emily L Naden, Phoebe E Blair, Frankjel A Hernandez Frometa, Felipe Karam Teixeira

{"title":"Destabilisation of bam transcripts terminates the mitotic phase of Drosophila female germline differentiation.","authors":"Tamsin J Samuels, Elizabeth J Torley, Valeriia Nadmitova, Emily L Naden, Phoebe E Blair, Frankjel A Hernandez Frometa, Felipe Karam Teixeira","doi":"10.1242/dev.204405","DOIUrl":"10.1242/dev.204405","url":null,"abstract":"The tight control of the mitotic phase of differentiation is crucial to prevent tumourigenesis while securing tissue homeostasis. In the Drosophila female germline, differentiation involves precisely four mitotic divisions, and accumulating evidence suggests that bag of marbles (bam), the initiator of differentiation, is also involved in controlling the number of divisions. To test this hypothesis, we depleted Bam from differentiating cells and found a reduced number of mitotic divisions. We examined the regulation of Bam using RNA imaging methods and found that the bam 3' UTR conveys instability to the transcript in the eight-cell cyst and early 16-cell cyst. We show that the RNA-binding protein Rbp9 is responsible for timing bam mRNA decay. Rbp9 itself is part of a sequential cascade of RNA-binding proteins activated downstream of Bam, and we show that it is regulated through a change in transcription start site, driven by Rbfox1. Altogether, we propose a model in which Bam expression at the beginning of differentiation initiates a series of events that eventually terminates the Bam expression domain.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143440303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cilia directionality reveals a slow reverse movement of principal neurons for positioning and lamina refinement in the cerebral cortex. 纤毛方向性揭示了大脑皮层中主要神经元的缓慢反向运动，用于定位和层状细化。

IF 3.7 2区生物学

Development Pub Date : 2025-03-01 Epub Date: 2025-03-11 DOI: 10.1242/dev.204300

Juan Yang, Soheila Mirhosseiniardakani, Liyan Qiu, Kostandina Bicja, Abigail Del Greco, Kevin JungKai Lin, Mark Lyon, Xuanmao Chen

{"title":"Cilia directionality reveals a slow reverse movement of principal neurons for positioning and lamina refinement in the cerebral cortex.","authors":"Juan Yang, Soheila Mirhosseiniardakani, Liyan Qiu, Kostandina Bicja, Abigail Del Greco, Kevin JungKai Lin, Mark Lyon, Xuanmao Chen","doi":"10.1242/dev.204300","DOIUrl":"https://doi.org/10.1242/dev.204300","url":null,"abstract":"Currently, not much is known about neuronal positioning and the roles of primary cilia in postnatal neurodevelopment. We show that primary cilia of principal neurons undergo marked changes in positioning and orientation, concurrent with postnatal neuron positioning in the mouse cerebral cortex. Primary cilia of early- and late-born principal neurons in compact layers display opposite orientations, while neuronal primary cilia in loose laminae are predominantly oriented toward the pia. In contrast, astrocytes and interneurons, and neurons in nucleated brain regions do not display specific cilia directionality. We further discovered that the cell bodies of principal neurons in inside-out laminated regions spanning from the hippocampal CA1 region to neocortex undergo a slow 'reverse movement' for postnatal positioning and lamina refinement. Furthermore, selective disruption of cilia function in the forebrain leads to altered lamination and gyrification in the retrosplenial cortex that is formed by reverse movement. Collectively, this study identifies reverse movement as a fundamental process for principal cell positioning that refines lamination in the cerebral cortex and casts light on the evolutionary transition from three-layered allocortices to six-layered neocortices.","PeriodicalId":11375,"journal":{"name":"Development","volume":"152 5","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143604180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The people behind the papers - Matthew Kourakis and William Smith. 这些文件背后的人——马修·库拉基斯和威廉·史密斯。

IF 3.7 2区生物学

Development Pub Date : 2025-03-01 Epub Date: 2025-03-13 DOI: 10.1242/dev.204736

引用次数: 0

POU4F2 overexpression promotes the genesis of retinal ganglion cell-like projection neurons from late progenitors. POU4F2/BRN3B过表达促进视网膜神经节细胞样投射神经元的发生。

IF 3.7 2区生物学

Development Pub Date : 2025-03-01 Epub Date: 2025-03-03 DOI: 10.1242/dev.204297

Viviane Medeiros Oliveira-Valença, Jacqueline Marie Roberts, Vitória Melo Fernandes-Cerqueira, Carolina Herkenhoff Colmerauer, Beatriz Cardoso de Toledo, Pedro Lucas Santos-França, Rafael Linden, Rodrigo Alves Portela Martins, Maurício Rocha-Martins, Alejandra Bosco, Monica Lynn Vetter, Mariana Souza da Silveira

{"title":"POU4F2 overexpression promotes the genesis of retinal ganglion cell-like projection neurons from late progenitors.","authors":"Viviane Medeiros Oliveira-Valença, Jacqueline Marie Roberts, Vitória Melo Fernandes-Cerqueira, Carolina Herkenhoff Colmerauer, Beatriz Cardoso de Toledo, Pedro Lucas Santos-França, Rafael Linden, Rodrigo Alves Portela Martins, Maurício Rocha-Martins, Alejandra Bosco, Monica Lynn Vetter, Mariana Souza da Silveira","doi":"10.1242/dev.204297","DOIUrl":"10.1242/dev.204297","url":null,"abstract":"Retinal ganglion cells (RGCs) are the projection neurons of the retina, and their death promotes an irreversible blindness. Several factors were described to control their genesis during retinal development. These include Atoh7, a major orchestrator of the RGC program, and downstream targets of this transcription factor, including Pou4f factors, that in turn regulate key aspects of terminal differentiation. The absence of POU4F family genes results in defects in RGC differentiation, aberrant axonal elaboration and, ultimately, RGC death. This confirms the requirement of POU4F factors for RGC development and survival, with a crucial role in regulating RGC axon outgrowth and pathfinding. Here, we have investigated in vivo whether ectopic Pou4f2 expression in late retinal progenitor cells (late RPCs) is sufficient to induce the generation of cells with RGC properties, including long-range axon projections. We show that Pou4f2 overexpression generates RGC-like cells that share morphological and transcriptional features with RGCs that are normally generated during early development and extend axonal projections up to the brain. In conclusion, these results show that POU4F2 alone is sufficient to promote the crucial properties of projection neurons that arise from retinal progenitors outside their developmental window.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143413505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The people behind the papers - Christopher De Bono, Stéphane Zaffran and Heather Etchevers. 这些文件背后的人——克里斯托弗·德·博诺、斯特姆·扎弗兰和希瑟·埃切弗斯。

IF 3.7 2区生物学

Development Pub Date : 2025-03-01 Epub Date: 2025-03-12 DOI: 10.1242/dev.204734

引用次数: 0

Activation of prep expression by Tet2 promotes the proliferation of bipotential progenitor cells during liver regeneration. Tet2激活prep表达可促进肝再生过程中双电位祖细胞的增殖。

IF 3.7 2区生物学

Development Pub Date : 2025-03-01 Epub Date: 2025-03-13 DOI: 10.1242/dev.204339

Kun Jia, Bo Cheng, Lirong Huang, Jiaxin Xu, Fasheng Liu, Xinjun Liao, Kai Liao, Huiqiang Lu

{"title":"Activation of prep expression by Tet2 promotes the proliferation of bipotential progenitor cells during liver regeneration.","authors":"Kun Jia, Bo Cheng, Lirong Huang, Jiaxin Xu, Fasheng Liu, Xinjun Liao, Kai Liao, Huiqiang Lu","doi":"10.1242/dev.204339","DOIUrl":"10.1242/dev.204339","url":null,"abstract":"Biliary epithelial cell (BEC)-derived liver regeneration in zebrafish exhibits similarities to liver regeneration in chronic liver injury. However, the underlying mechanisms remain poorly understood. Here, we identified a serine peptidase called prolyl endopeptidase (Prep) as an indispensable factor during the BEC-derived liver regeneration process. prep was significantly upregulated and enriched in bipotential progenitor cells (BP-PCs). Through gain- and loss-of-function assays, prep was found to potently accelerate liver regeneration and drastically increase the proliferation of BP-PCs. Mechanistically, prep expression was directly regulated by ten-eleven translocation 2 (Tet2)-mediated DNA demethylation. More strikingly, Tet2 regulated prep expression by directly interacting and reducing the methylation of CpG sites in the prep promoter. Subsequently, Prep activated the PI3K-AKT-mTOR signaling pathway to regulate liver regeneration. Therefore, our study revealed the role and mechanism of Tet2-mediated DNA demethylation-associated upregulation of prep in the proliferation of BP-PCs during liver regeneration. These results identify promising targets for stimulating regeneration following chronic liver injury.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143457146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Arabidopsis bHLH60 can regulate leaf development by interacting with and counteracting the AS1-AS2 complex to affect BP1 expression. 拟南芥bHLH60通过与AS1-AS2复合体相互作用和拮抗，影响BP1的表达，从而调控叶片发育。

IF 3.7 2区生物学

Development Pub Date : 2025-03-01 Epub Date: 2025-03-12 DOI: 10.1242/dev.204633

Qinqin Chen, Min Deng, Peiyu Zhao, Muhammad Saad Rehmani, Wenpeng Cheng, Shuangshuang Wang, Jing Wang, Chen Wang, Shidong Gao, Bo Yang, Michael K Deyholos, Yuan-Qing Jiang

{"title":"Arabidopsis bHLH60 can regulate leaf development by interacting with and counteracting the AS1-AS2 complex to affect BP1 expression.","authors":"Qinqin Chen, Min Deng, Peiyu Zhao, Muhammad Saad Rehmani, Wenpeng Cheng, Shuangshuang Wang, Jing Wang, Chen Wang, Shidong Gao, Bo Yang, Michael K Deyholos, Yuan-Qing Jiang","doi":"10.1242/dev.204633","DOIUrl":"10.1242/dev.204633","url":null,"abstract":"During leaf morphogenesis, various factors interplay to mediate abaxial-adaxial and proximal-distal polarity, along with other factors contributing to organ boundary and leaf expansion. Although significant progress has been made in understanding the genetics of leaf development, there are still gaps in our understanding of leaf morphogenesis. Here, we show that the bHLH60 transcription factor can affect leaf development. Overexpression of bHLH60 leads to pleiotropic phenotypes with increased leaf serration and reduced fertility. A RNA-seq analysis showed that the BREVIPEDICELLUS1 (BP1) gene implicated in leaf development was upregulated as a result of bHLH60 overexpression. Further analysis revealed that bHLH60 directly bound to the BP1 promoter to activate its transcription. Subsequently, bHLH60 interacted with the repressor complex AS1-AS2 and JLO to relieve the inhibition of AS1-AS2 on BP1, thereby indirectly activating the expression of BP1. Genetic analysis indicated that the BP1 mutation partially suppressed the phenotype resulting from bHLH60 overexpression, thereby concluding that the pleiotropic phenotypes of bHLH60-overexpressing plants were partly dependent on BP1. We propose that bHLH60 competes with the AS1-AS2-JLO complex to regulate BP1 expression to modulate leaf development.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143499759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

EFL-3/E2F7 modulates Wnt signalling by repressing the Nemo-like kinase LIT-1 during asymmetric epidermal cell division in Caenorhabditis elegans. 在秀丽隐杆线虫不对称表皮细胞分裂过程中，EFL-3/E2F7通过抑制nemo样激酶lit1调控Wnt信号。

IF 3.7 2区生物学

Development Pub Date : 2025-03-01 Epub Date: 2025-03-03 DOI: 10.1242/dev.204546

Mar Ferrando-Marco, Michalis Barkoulas

引用次数: 0

Transitions in development - an interview with Chris Whitewoods. 开发中的过渡——对Chris Whitewoods的采访

IF 3.7 2区生物学

Development Pub Date : 2025-03-01 Epub Date: 2025-03-03 DOI: 10.1242/dev.204723

引用次数: 0

Motor neurons in the tunicate caudal central nervous system reveal homology to the vertebrate spinal cord. 尾状中枢神经系统的运动神经元与脊椎动物脊髓具有同源性。

IF 3.7 2区生物学

Development Pub Date : 2025-03-01 Epub Date: 2025-03-13 DOI: 10.1242/dev.204525

Matthew J Kourakis, Kerrianne Ryan, Erin D Newman-Smith, Ian A Meinertzhagen, William C Smith

引用次数: 0