Yan Zhang, Ying Feng, Xiaolei Ye, Yi Lin, Ling Zeng, Yuting Luo, Lin Zhou, Chenghao Shen, Weiqi Hu, Dong Yan, Xinhua Lin
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The Drosophila Dpp/BMP signaling directly regulates dpp transcription for optimal ligand dosage.
Precise control of morphogen dosage in the source is essential for establishing developmental patterning and maintaining signaling stability. Here, we demonstrate that in Drosophila wing imaginal discs, the Dpp/BMP signaling modulates its ligand Dpp (Decapentaplegic) expression through direct transcriptional auto-regulation. The results show that reduction of Dpp/BMP signaling upregulates dpp transcription, whereas enhanced signaling represses it. This transcriptional negative feedback mechanism primarily depends on the BMP-Silencer-Element (BMP-SE) complex binding to the BMP-SE motifs at the dpp downstream distal cis-regulatory region. We validated this mechanism through BMP-SE complex interference and BMP-SE motif disruption assays. Our findings extend the morphogen source-sink theory by uncovering that the Dpp/BMP signaling pathway dynamically balances Dpp dosage via transcriptional auto-modulation, thereby ensuring optimal ligand production for signaling homeostasis.
期刊介绍：
Development’s scope covers all aspects of plant and animal development, including stem cell biology and regeneration. The single most important criterion for acceptance in Development is scientific excellence. Research papers (articles and reports) should therefore pose and test a significant hypothesis or address a significant question, and should provide novel perspectives that advance our understanding of development. We also encourage submission of papers that use computational methods or mathematical models to obtain significant new insights into developmental biology topics. Manuscripts that are descriptive in nature will be considered only when they lay important groundwork for a field and/or provide novel resources for understanding developmental processes of broad interest to the community.
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To aid navigability, Development has dedicated sections of the journal to stem cells & regeneration and to human development. The criteria for acceptance into these sections is identical to those outlined above. Authors and editors are encouraged to nominate appropriate manuscripts for inclusion in one of these sections.
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