The Drosophila Dpp/BMP signaling directly regulates dpp transcription for optimal ligand dosage.

IF 3.6 2区生物学 Q1 DEVELOPMENTAL BIOLOGY

Development Pub Date : 2025-09-16 DOI:10.1242/dev.204488

Yan Zhang, Ying Feng, Xiaolei Ye, Yi Lin, Ling Zeng, Yuting Luo, Lin Zhou, Chenghao Shen, Weiqi Hu, Dong Yan, Xinhua Lin

引用次数: 0

Abstract

Precise control of morphogen dosage in the source is essential for establishing developmental patterning and maintaining signaling stability. Here, we demonstrate that in Drosophila wing imaginal discs, the Dpp/BMP signaling modulates its ligand Dpp (Decapentaplegic) expression through direct transcriptional auto-regulation. The results show that reduction of Dpp/BMP signaling upregulates dpp transcription, whereas enhanced signaling represses it. This transcriptional negative feedback mechanism primarily depends on the BMP-Silencer-Element (BMP-SE) complex binding to the BMP-SE motifs at the dpp downstream distal cis-regulatory region. We validated this mechanism through BMP-SE complex interference and BMP-SE motif disruption assays. Our findings extend the morphogen source-sink theory by uncovering that the Dpp/BMP signaling pathway dynamically balances Dpp dosage via transcriptional auto-modulation, thereby ensuring optimal ligand production for signaling homeostasis.

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果蝇Dpp/BMP信号直接调节Dpp转录以获得最佳配体剂量。

在源头上精确控制形态素的剂量对于建立发育模式和维持信号稳定性至关重要。在本研究中，我们证明了在果蝇翅膀成像盘中，Dpp/BMP信号通过直接转录自调节来调节其配体Dpp （Decapentaplegic）的表达。结果表明，Dpp/BMP信号的减少上调了Dpp的转录，而信号的增强抑制了Dpp的转录。这种转录负反馈机制主要依赖于bmp -沉默元件（BMP-SE）复合物与dpp下游远端顺式调控区BMP-SE基序的结合。我们通过BMP-SE复合物干扰和BMP-SE基序破坏分析验证了这一机制。通过揭示Dpp/BMP信号通路通过转录自调节动态平衡Dpp剂量，从而确保信号稳态所需的最佳配体产生，我们的研究结果扩展了形态源-汇理论。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Development 生物-发育生物学

CiteScore

6.70

自引率

4.30%

发文量

433

审稿时长

3 months

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