Development最新文献_第2页

Planar cell polarity zebrafish models of congenital scoliosis reveal underlying defects in notochord morphogenesis. 先天性脊柱侧凸的平面细胞极性斑马鱼模型揭示了脊索形态发生的新的潜在缺陷。

IF 3.7 2区生物学

Development Pub Date : 2024-11-01 Epub Date: 2024-11-12 DOI: 10.1242/dev.202829

Mingqin Wang, Sen Zhao, Chenjun Shi, Marie-Claude Guyot, Meijiang Liao, Josephine T Tauer, Bettina M Willie, Nikita Cobetto, Carl-Éric Aubin, Elke Küster-Schöck, Pierre Drapeau, Jitao Zhang, Nan Wu, Zoha Kibar

{"title":"Planar cell polarity zebrafish models of congenital scoliosis reveal underlying defects in notochord morphogenesis.","authors":"Mingqin Wang, Sen Zhao, Chenjun Shi, Marie-Claude Guyot, Meijiang Liao, Josephine T Tauer, Bettina M Willie, Nikita Cobetto, Carl-Éric Aubin, Elke Küster-Schöck, Pierre Drapeau, Jitao Zhang, Nan Wu, Zoha Kibar","doi":"10.1242/dev.202829","DOIUrl":"10.1242/dev.202829","url":null,"abstract":"Congenital scoliosis (CS) is a type of vertebral malformation for which the etiology remains elusive. The notochord is pivotal for vertebrae development, but its role in CS is still understudied. Here, we generated a zebrafish knockout of ptk7a, a planar cell polarity (PCP) gene that is essential for convergence and extension (C&E) of the notochord, and detected congenital scoliosis-like vertebral malformations (CVMs). Maternal zygotic ptk7a mutants displayed severe C&E defects of the notochord. Excessive apoptosis occurred in the malformed notochord, causing a significantly reduced number of vacuolated cells, and compromising the mechanical properties of the notochord. The latter manifested as a less-stiff extracellular matrix along with a significant reduction in the number of the caveolae and severely loosened intercellular junctions in the vacuolated region. These defects led to focal kinks, abnormal mineralization, and CVMs exclusively at the anterior spine. Loss of function of another PCP gene, vangl2, also revealed excessive apoptosis in the notochord associated with CVMs. This study suggests a new model for CS pathogenesis that is associated with defects in notochord C&E and highlights an essential role of PCP signaling in vertebrae development.","PeriodicalId":11375,"journal":{"name":"Development","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142460557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evolution and development of complex floral displays. 复杂花卉展示的进化和发展。

IF 3.7 2区生物学

Development Pub Date : 2024-11-01 Epub Date: 2024-11-05 DOI: 10.1242/dev.203027

Farahnoz N Khojayori, Udhaya Ponraj, Kristina Buch, Yi Zhao, Humberto Herrera-Ubaldo, Beverley J Glover

{"title":"Evolution and development of complex floral displays.","authors":"Farahnoz N Khojayori, Udhaya Ponraj, Kristina Buch, Yi Zhao, Humberto Herrera-Ubaldo, Beverley J Glover","doi":"10.1242/dev.203027","DOIUrl":"https://doi.org/10.1242/dev.203027","url":null,"abstract":"Flowering plants - angiosperms - display an astounding diversity of floral features, which have evolved in response to animal pollination and have resulted in the most species-rich plant clade. Combinations of macroscale (e.g. colour, symmetry, organ number) and microscale (e.g. cell type, tissue patterning) features often lead to highly elaborate floral displays. Most studies have focused on model species with simple floral displays to uncover the genetic and evolutionary mechanisms involved in flower evolution, yet few studies have focused on complex floral displays. Here, we review current knowledge on the development and evolution of complex floral displays. We review gene regulatory networks involved in four developmental pathways contributing to overall floral display (inflorescence architecture, organ identity, flower symmetry and flower colour) in classical plant models. We then discuss how evolutionary modification of one or more of these pathways has resulted in the production of a range of complex floral displays. Finally, we explore modular systems in which multiple pathways have been modified simultaneously, generating the most elaborate floral displays.","PeriodicalId":11375,"journal":{"name":"Development","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142582299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nuclear instance segmentation and tracking for preimplantation mouse embryos. 植入前小鼠胚胎的核实例分割和跟踪。

IF 3.7 2区生物学

Development Pub Date : 2024-11-01 Epub Date: 2024-11-06 DOI: 10.1242/dev.202817

Hayden Nunley, Binglun Shao, David Denberg, Prateek Grover, Jaspreet Singh, Maria Avdeeva, Bradley Joyce, Rebecca Kim-Yip, Abraham Kohrman, Abhishek Biswas, Aaron Watters, Zsombor Gal, Alison Kickuth, Madeleine Chalifoux, Stanislav Y Shvartsman, Lisa M Brown, Eszter Posfai

{"title":"Nuclear instance segmentation and tracking for preimplantation mouse embryos.","authors":"Hayden Nunley, Binglun Shao, David Denberg, Prateek Grover, Jaspreet Singh, Maria Avdeeva, Bradley Joyce, Rebecca Kim-Yip, Abraham Kohrman, Abhishek Biswas, Aaron Watters, Zsombor Gal, Alison Kickuth, Madeleine Chalifoux, Stanislav Y Shvartsman, Lisa M Brown, Eszter Posfai","doi":"10.1242/dev.202817","DOIUrl":"10.1242/dev.202817","url":null,"abstract":"For investigations into fate specification and morphogenesis in time-lapse images of preimplantation embryos, automated 3D instance segmentation and tracking of nuclei are invaluable. Low signal-to-noise ratio, high voxel anisotropy, high nuclear density, and variable nuclear shapes can limit the performance of segmentation methods, while tracking is complicated by cell divisions, low frame rates, and sample movements. Supervised machine learning approaches can radically improve segmentation accuracy and enable easier tracking, but they often require large amounts of annotated 3D data. Here, we first report a previously unreported mouse line expressing near-infrared nuclear reporter H2B-miRFP720. We then generate a dataset (termed BlastoSPIM) of 3D images of H2B-miRFP720-expressing embryos with ground truth for nuclear instances. Using BlastoSPIM, we benchmark seven convolutional neural networks and identify Stardist-3D as the most accurate instance segmentation method. With our BlastoSPIM-trained Stardist-3D models, we construct a complete pipeline for nuclear instance segmentation and lineage tracking from the eight-cell stage to the end of preimplantation development (>100 nuclei). Finally, we demonstrate the usefulness of BlastoSPIM as pre-train data for related problems, both for a different imaging modality and for different model systems.","PeriodicalId":11375,"journal":{"name":"Development","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142380273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Symmetry breaking and fate divergence during lateral inhibition in Drosophila. 果蝇横向抑制过程中的对称性破坏和命运分化

IF 3.7 2区生物学

Development Pub Date : 2024-11-01 Epub Date: 2024-11-08 DOI: 10.1242/dev.203165

Minh-Son Phan, Jang-Mi Kim, Cara Picciotto, Lydie Couturier, Nisha Veits, Khallil Mazouni, François Schweisguth

{"title":"Symmetry breaking and fate divergence during lateral inhibition in Drosophila.","authors":"Minh-Son Phan, Jang-Mi Kim, Cara Picciotto, Lydie Couturier, Nisha Veits, Khallil Mazouni, François Schweisguth","doi":"10.1242/dev.203165","DOIUrl":"10.1242/dev.203165","url":null,"abstract":"Lateral inhibition mediates alternative cell fate decision and produces regular cell fate patterns with fate symmetry breaking (SB) relying on the amplification of small stochastic differences in Notch activity via an intercellular negative-feedback loop. Here, we used quantitative live imaging of endogenous Scute (Sc), a proneural factor, and of a Notch activity reporter to study the emergence of sensory organ precursor cells in the pupal abdomen of Drosophila. SB was observed at low Sc levels and was not preceded by a phase of intermediate Sc expression and Notch activity. Thus, mutual inhibition may only be transient in this context. In support of the intercellular feedback loop model, cell-to-cell variations in Sc levels promoted fate divergence. The size of the apical area of competing cells did not detectably bias this fate choice. Surprisingly, cells that were in direct contact at the time of SB could adopt the sensory organ precursor cell fate, albeit at low frequency (10%). These lateral inhibition defects were corrected by cellular rearrangements, not cell fate change, highlighting the role of cell-cell intercalation in pattern refinement.","PeriodicalId":11375,"journal":{"name":"Development","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142380274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Axin1 regulates tooth root development by inhibiting AKT1-mTORC1 activation and Shh translation in Hertwig's epithelial root sheath. Axin1 通过抑制 Hertwig 上皮根鞘中 AKT1-mTORC1 的激活和 Shh 的翻译来调节牙根的发育。

IF 3.7 2区生物学

Development Pub Date : 2024-11-01 Epub Date: 2024-10-30 DOI: 10.1242/dev.202899

Xiaoyu Zheng, Hongcan Huang, Zhipeng Zhou, Weihua Guo, Guobin Yang, Zhi Chen, Di Chen, YiPing Chen, Guohua Yuan

{"title":"Axin1 regulates tooth root development by inhibiting AKT1-mTORC1 activation and Shh translation in Hertwig's epithelial root sheath.","authors":"Xiaoyu Zheng, Hongcan Huang, Zhipeng Zhou, Weihua Guo, Guobin Yang, Zhi Chen, Di Chen, YiPing Chen, Guohua Yuan","doi":"10.1242/dev.202899","DOIUrl":"10.1242/dev.202899","url":null,"abstract":"Hertwig's epithelial root sheath (HERS) interacts with dental apical mesenchyme and guides development of the tooth root, which is integral to the function of the whole tooth. However, the key genes in HERS essential for root development are understudied. Here, we show that Axin1, a scaffold protein that negatively regulates canonical Wnt signaling, is strongly expressed in the HERS. Axin1 ablation in the HERS of mice leads to defective root development, but in a manner independent of canonical Wnt signaling. Further studies reveal that Axin1 in the HERS negatively regulates the AKT1-mTORC1 pathway through binding to AKT1, leading to inhibition of ribosomal biogenesis and mRNA translation. Sonic hedgehog (Shh) protein, a morphogen essential for root development, is over-synthesized by upregulated mTORC1 activity upon Axin1 inactivation. Importantly, either haploinsufficiency of the mTORC1 subunit Rptor or pharmacological inhibition of Shh signaling can rescue the root defects in Axin1 mutant mice. Collectively, our data suggest that, independently of canonical Wnt signaling, Axin1 controls ribosomal biogenesis and selective mRNA translation programs via AKT1-mTORC1 signaling during tooth root development.","PeriodicalId":11375,"journal":{"name":"Development","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142343714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ribosome biogenesis is essential for hemogenic endothelial cells to generate hematopoietic stem cells. 核糖体的生物发生对造血内皮细胞生成造血干细胞至关重要。

IF 3.7 2区生物学

Development Pub Date : 2024-11-01 Epub Date: 2024-10-23 DOI: 10.1242/dev.202875

Di Liu, Haizhen Wang, Haifeng Chen, Xitong Tian, Yuqing Jiao, Chi Wang, Yuhui Li, Zongcheng Li, Siyuan Hou, Yanli Ni, Bing Liu, Yu Lan, Jie Zhou

{"title":"Ribosome biogenesis is essential for hemogenic endothelial cells to generate hematopoietic stem cells.","authors":"Di Liu, Haizhen Wang, Haifeng Chen, Xitong Tian, Yuqing Jiao, Chi Wang, Yuhui Li, Zongcheng Li, Siyuan Hou, Yanli Ni, Bing Liu, Yu Lan, Jie Zhou","doi":"10.1242/dev.202875","DOIUrl":"10.1242/dev.202875","url":null,"abstract":"Undergoing endothelial-to-hematopoietic transition, a small fraction of embryonic aortic endothelial cells specializes into hemogenic endothelial cells (HECs) and eventually gives rise to hematopoietic stem cells (HSCs). Previously, we found that the activity of ribosome biogenesis (RiBi) is highly enriched in the HSC-primed HECs compared with adjacent arterial endothelial cells; however, whether RiBi is required in HECs for the generation of HSCs remains to be determined. Here, we have found that robust RiBi is markedly augmented during the endothelial-to-hematopoietic transition in mouse. Pharmacological inhibition of RiBi completely impeded the generation of HSCs in explant cultures. Moreover, disrupting RiBi selectively interrupted the HSC generation potential of HECs rather than T1 pre-HSCs, which was in line with its influence on cell cycle activity. Further investigation revealed that, upon HEC specification, the master transcription factor Runx1 dramatically bound to the loci of genes involved in RiBi, thereby facilitating this biological process. Taken together, our study provides functional evidence showing the indispensable role of RiBi in generating HSCs from HECs, providing previously unreported insights that may contribute to the improvement of HSC regeneration strategies.","PeriodicalId":11375,"journal":{"name":"Development","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11529273/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142343717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

40 years since the Heidelberg genetic screen that revolutionized developmental and cell biology. 海德堡基因筛选彻底改变了发育生物学和细胞生物学，距今已有 40 年。

IF 3.7 2区生物学

Development Pub Date : 2024-11-01 Epub Date: 2024-10-21 DOI: 10.1242/dev.204433

Mark Peifer

引用次数: 0

David Ish-Horowicz FRS (1948-2024): outstanding scientific discovery, with a unique touch of selfless humanity. 戴维-伊什霍洛维奇（David Ish-Horowicz FRS，1948-2024 年）：杰出的科学发现，独特的无私人性。

IF 3.7 2区生物学

Development Pub Date : 2024-11-01 Epub Date: 2024-10-21 DOI: 10.1242/dev.204429

Ilan Davis

引用次数: 0

Common modes of ERK induction resolve into context-specific signalling via emergent networks and cell-type-specific transcriptional repression. ERK诱导的常见模式可通过新兴网络和特定细胞类型的转录抑制转化为针对具体情况的信号。

IF 3.7 2区生物学

Development Pub Date : 2024-11-01 Epub Date: 2024-10-30 DOI: 10.1242/dev.202842

Marta Perera, Joshua M Brickman

{"title":"Common modes of ERK induction resolve into context-specific signalling via emergent networks and cell-type-specific transcriptional repression.","authors":"Marta Perera, Joshua M Brickman","doi":"10.1242/dev.202842","DOIUrl":"10.1242/dev.202842","url":null,"abstract":"Fibroblast Growth Factor signalling via ERK exerts diverse roles in development and disease. In mammalian preimplantation embryos and naïve pluripotent stem cells ERK promotes differentiation, whereas in primed pluripotent states closer to somatic differentiation ERK sustains self-renewal. How can the same pathway produce different outcomes in two related cell types? To explore context-dependent ERK signalling we generated cell and mouse lines that allow for tissue- and time-specific ERK activation. Using these tools, we find that specificity in ERK response is mostly mediated by repression of transcriptional targets that occur in tandem with reductions in chromatin accessibility at regulatory regions. Furthermore, immediate early ERK responses are largely shared by different cell types but produce cell-specific programmes as these responses interface with emergent networks in the responding cells. Induction in naïve pluripotency is accompanied by chromatin changes, whereas in later stages it is not, suggesting that chromatin context does not shape signalling response. Altogether, our data suggest that cell-type-specific responses to ERK signalling exploit the same immediate early response, but then sculpt it to specific lineages via repression of distinct cellular programmes.","PeriodicalId":11375,"journal":{"name":"Development","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142497087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In preprints: early cell differentiation and mechanical environment. 预印本：早期细胞分化与机械环境。

IF 3.7 2区生物学

Development Pub Date : 2024-11-01 Epub Date: 2024-10-21 DOI: 10.1242/dev.204417

Corentin Mollier, Jean-Léon Maître

引用次数: 0