Development最新文献_第2页

Quantifying the relationship between cell proliferation and morphology during development of the face. 定量测定面部发育过程中细胞增殖与形态的关系。

IF 3.7 2区生物学

Development Pub Date : 2025-04-01 Epub Date: 2025-04-07 DOI: 10.1242/dev.204511

Lucas D Lo Vercio, Rebecca M Green, Andreas Dauter, Elizabeth C Barretto, Marta Vidal-García, Jay Devine, Marta Marchini, Samuel Robertson, Xiang Zhao, Anandita Mahika, M Bilal Shakir, Sienna Guo, Julia C Boughner, Heather Szabo-Rogers, Wendy Dean, Arthur D Lander, Ralph S Marcucio, Nils D Forkert, Benedikt Hallgrímsson

{"title":"Quantifying the relationship between cell proliferation and morphology during development of the face.","authors":"Lucas D Lo Vercio, Rebecca M Green, Andreas Dauter, Elizabeth C Barretto, Marta Vidal-García, Jay Devine, Marta Marchini, Samuel Robertson, Xiang Zhao, Anandita Mahika, M Bilal Shakir, Sienna Guo, Julia C Boughner, Heather Szabo-Rogers, Wendy Dean, Arthur D Lander, Ralph S Marcucio, Nils D Forkert, Benedikt Hallgrímsson","doi":"10.1242/dev.204511","DOIUrl":"10.1242/dev.204511","url":null,"abstract":"Morphogenesis requires highly coordinated, complex interactions between cellular processes: proliferation, migration and apoptosis, along with physical tissue interactions. How these cellular and tissue dynamics drive morphogenesis remains elusive. Three dimensional (3D) microscopic imaging holds great promise, and generates elegant images, but generating even moderate throughput for quantified images is challenging for many reasons. As a result, the association between morphogenesis and cellular processes in 3D developing tissues has not been fully explored. To address this gap, we have developed an imaging and image analysis pipeline to enable 3D quantification of cellular dynamics along with 3D morphology for the same individual embryo. Specifically, we focus on how 3D distribution of proliferation relates to morphogenesis during mouse facial development. Our method involves imaging with light-sheet microscopy, automated segmentation of cells and tissues using machine learning-based tools, and quantification of external morphology by geometric morphometrics. Applying this framework, we show that changes in proliferation are tightly correlated with changes in morphology over the course of facial morphogenesis. These analyses illustrate the potential of this pipeline to investigate mechanistic relationships between cellular dynamics and morphogenesis during embryonic development.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143482526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In preprints: an epigenetic clock that does not tick. 在预印本中：一个不滴答作响的表观遗传时钟。

IF 3.7 2区生物学

Development Pub Date : 2025-04-01 Epub Date: 2025-03-31 DOI: 10.1242/dev.204780

Lenka Gahurova

引用次数: 0

Muscle and intestine innexins with muscle Deg/Enac channels promote muscle coordination and embryo elongation. 具有肌Deg/Enac通道的肌内蛋白和肠内蛋白促进肌肉协调和胚胎伸长。

IF 3.7 2区生物学

Development Pub Date : 2025-03-27 DOI: 10.1242/dev.204242

Flora Llense, Teresa Ferraro, Xinyi Yang, Hanla Song, Michel Labouesse

{"title":"Muscle and intestine innexins with muscle Deg/Enac channels promote muscle coordination and embryo elongation.","authors":"Flora Llense, Teresa Ferraro, Xinyi Yang, Hanla Song, Michel Labouesse","doi":"10.1242/dev.204242","DOIUrl":"https://doi.org/10.1242/dev.204242","url":null,"abstract":"Body axis elongation represents a fundamental morphogenetic process in development, which involves cell shape changes powered by mechanical forces. How mechanically interconnected tissues coordinate in organismal development remains largely unexplored. During C. elegans elongation, cyclic forces generated by muscle contractions induce remodeling of adherens junctions and the actin cytoskeleton in the epidermis, facilitating gradual embryo lengthening. While previous studies have identified key players in epidermal cells, understanding how muscle cells coordinate their activity for proper embryo elongation remains unsolved. Using a Calcium sensor to monitor muscle activity during elongation, we identified two cells in each muscle quadrant with a leader cell function that orchestrate muscle activity within their respective quadrants. Strikingly, ablation of these cells halted muscle contractions and delayed elongation. A targeted RNAi screen focusing on communication channels identified two innexins and two Deg channels regulating muscle activity, which proved required for normal embryonic elongation. Interestingly, one innexin exhibits specific expression in intestinal cells. Our findings provide novel insights into how embryonic body wall muscles coordinate their activity and how interconnected tissues ensure proper morphogenesis.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143729330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optogenetic control of Nodal signaling patterns. 节点信号模式的光遗传控制。

IF 3.7 2区生物学

Development Pub Date : 2025-03-27 DOI: 10.1242/dev.204506

Harold M McNamara, Alison M Guyer, Bill Z Jia, Vicente J Parot, Caleb D Dobbs, Alexander F Schier, Adam E Cohen, Nathan D Lord

{"title":"Optogenetic control of Nodal signaling patterns.","authors":"Harold M McNamara, Alison M Guyer, Bill Z Jia, Vicente J Parot, Caleb D Dobbs, Alexander F Schier, Adam E Cohen, Nathan D Lord","doi":"10.1242/dev.204506","DOIUrl":"10.1242/dev.204506","url":null,"abstract":"A crucial step in early embryogenesis is the establishment of spatial patterns of signaling activity. Tools to perturb morphogen signals with high resolution in space and time can help reveal how embryonic cells decode these signals to make appropriate fate decisions. Here, we present new optogenetic reagents and an experimental pipeline for creating designer Nodal signaling patterns in live zebrafish embryos. Nodal receptors were fused to the light-sensitive heterodimerizing pair Cry2/CIB1N, and the Type II receptor was sequestered to the cytosol. The improved optoNodal2 reagents eliminate dark activity and improve response kinetics, without sacrificing dynamic range. We adapted an ultra-widefield microscopy platform for parallel light patterning in up to 36 embryos and demonstrated precise spatial control over Nodal signaling activity and downstream gene expression. Patterned Nodal activation drove precisely controlled internalization of endodermal precursors. Further, we used patterned illumination to generate synthetic signaling patterns in Nodal signaling mutants, rescuing several characteristic developmental defects. This study establishes an experimental toolkit for systematic exploration of Nodal signaling patterns in live embryos.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143718369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

GATA factor Serpent promotes phagocytosis in non-professional phagocytes during Drosophila oogenesis. GATA因子Serpent在果蝇卵发生过程中促进非专业吞噬细胞的吞噬。

IF 3.7 2区生物学

Development Pub Date : 2025-03-26 DOI: 10.1242/dev.204464

Baosheng Zeng, Haley Grayson, Jianjun Sun

{"title":"GATA factor Serpent promotes phagocytosis in non-professional phagocytes during Drosophila oogenesis.","authors":"Baosheng Zeng, Haley Grayson, Jianjun Sun","doi":"10.1242/dev.204464","DOIUrl":"https://doi.org/10.1242/dev.204464","url":null,"abstract":"Clearance of dying cells is essential for tissue homeostasis and requires both professional and non-professional phagocytes; however, it is unclear what promotes phagocytosis by non-professional phagocytes. Follicle cells of Drosophila egg chambers function as non-professional phagocytes to clear large germ cell debris in mid and late oogenesis, providing an excellent model for the study of non-professional phagocytes. Here we demonstrate that GATA factor Serpent (Srp) plays an indispensable role in promoting the phagocytic capacity of follicle cells in both processes. Srp is upregulated in follicle cells of degenerating mid-stage egg chambers, and its knockdown results in incomplete clearance of germ cell debris and premature follicle cell death. In addition, Srp is upregulated in stretch follicle cells and essential for clearing the nurse cell nuclei in late oogenesis. Genetic analysis reveals that Srp acts downstream of JNK signaling to upregulate the expression of the phagocytic receptor Draper (Drpr) as well as other components in the corpse processing machinery. Our findings highlight the crucial role for Srp in non-professional phagocytes during Drosophila oogenesis, which may also be conserved across species.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143709370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gαi2 Interaction with EB1 controls microtubule dynamics and Rac1 activity in Xenopus neural crest cell migration. Gαi2与EB1的相互作用控制爪蟾神经嵴细胞迁移的微管动力学和Rac1活性。

IF 3.7 2区生物学

Development Pub Date : 2025-03-26 DOI: 10.1242/dev.204235

Soraya Villaseca, Juan Ignacio Leal, Lina Mariana Tovar, María José Ruiz, Jossef Guajardo, Hernan Morales-Navarrete, Roberto Mayor, Marcela Torrejón

{"title":"Gαi2 Interaction with EB1 controls microtubule dynamics and Rac1 activity in Xenopus neural crest cell migration.","authors":"Soraya Villaseca, Juan Ignacio Leal, Lina Mariana Tovar, María José Ruiz, Jossef Guajardo, Hernan Morales-Navarrete, Roberto Mayor, Marcela Torrejón","doi":"10.1242/dev.204235","DOIUrl":"https://doi.org/10.1242/dev.204235","url":null,"abstract":"Cell migration is crucial in embryonic development, tissue repair, and cancer metastasis, driven by the actin and tubulin cytoskeletons that control cell shape, polarity, adhesion, and movement in response to various cues. Although heterotrimeric G proteins are known to be involved in cell migration, the specific mechanisms, especially during development, remain elusive. This study examines the role of Gαi2, a heterotrimeric G protein subunit, in cranial neural crest (NC) cell migration during embryonic development. Our research reveals that Gαi2 interacts directly with the microtubule-associated protein EB1, regulating microtubule dynamics. We show that Gαi2 knockdown stabilizes microtubules, disrupts cell polarity and morphology, increases Rac1-GTP at the leading edge and cell-cell contacts, and impairs actin localization and focal adhesion disassembly. Additionally, RhoA-GTP is reduced at cell-cell contacts and concentrated at the leading edge in Gαi2 knockdown cells, providing evidence of Gαi2 role in polarity. Nocodazole treatment, a microtubule-depolymerizing agent, reduces Rac1 activity, restoring cranial NC cell morphology, actin distribution, and overall migration. Our findings highlight Gαi2 crucial role in cranial NC cell migration by modulating microtubule dynamics through EB1 and Rac1 activity.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143709375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Live imaging in zebrafish reveals tissue-specific strategies for amoeboid migration. 斑马鱼的实时成像揭示了变形虫迁移的组织特异性策略。

IF 3.7 2区生物学

Development Pub Date : 2025-03-21 DOI: 10.1242/dev.204351

Tanner F Robertson, Jon Schrope, Zoe Zwick, Julie Rindy, Adam Horn, Yiran Hou, Anna Huttenlocher

引用次数: 0

Insulin-like growth factor 2 (IGF2) as a driving force of exponential expansion and differentiation of neonatal thymus. 胰岛素样生长因子2 （IGF2）作为新生儿胸腺指数扩张和分化的驱动力。

IF 3.7 2区生物学

Development Pub Date : 2025-03-20 DOI: 10.1242/dev.204347

Seung Woo Kang, Bryan R Helm, Yu Wang, Shiyun Xiao, Wen Zhang, Anusha Vasudev, Ken S Lau, Qi Liu, Ellen R Richie, Laura P Hale, Nancy R Manley

{"title":"Insulin-like growth factor 2 (IGF2) as a driving force of exponential expansion and differentiation of neonatal thymus.","authors":"Seung Woo Kang, Bryan R Helm, Yu Wang, Shiyun Xiao, Wen Zhang, Anusha Vasudev, Ken S Lau, Qi Liu, Ellen R Richie, Laura P Hale, Nancy R Manley","doi":"10.1242/dev.204347","DOIUrl":"https://doi.org/10.1242/dev.204347","url":null,"abstract":"Like all organs, the thymus grows in size and function rapidly during development, which comes to a halt after birth. However, the molecular mechanisms behind such a transition in the thymus remain obscure. Using single-cell RNA sequencing (scRNA-seq) of the murine thymic stroma, we identified that major transcriptomic changes occur in the endothelium and mesenchyme across the transition to homeostasis. Differentially expressed gene and intercellular network analyses of temporally resolved scRNA-seq data revealed fibroblast-derived insulin-like growth factor 2 (IGF2) as a candidate driving neonatal thymic expansion. We demonstrated IGF2 activity promotes a cortical TEC-specific proliferation and is tightly regulated at the thymic growth transition. Bulk RNA-seq of human thymi across the transition also revealed IGF2 to drive thymic expansion, suggesting an evolutionarily conserved role. Our study highlights the role of a fibroblast-derived IGF2 in promoting cTEC proliferation and differentiation, resulting in early thymic expansion that is followed by down-regulation to establish homeostasis.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Long-term labelling and tracing of endodermal cells using perpetual cycling Gal4-UAS system. 使用永久循环Gal4-UAS系统对内胚层细胞进行长期标记和追踪。

IF 3.7 2区生物学

Development Pub Date : 2025-03-19 DOI: 10.1242/dev.204289

Yanfeng Li, You Li, Bangzhuo Huang, Ruhao Zhang, Jianbo He, Lingfei Luo, Yun Yang

{"title":"Long-term labelling and tracing of endodermal cells using perpetual cycling Gal4-UAS system.","authors":"Yanfeng Li, You Li, Bangzhuo Huang, Ruhao Zhang, Jianbo He, Lingfei Luo, Yun Yang","doi":"10.1242/dev.204289","DOIUrl":"10.1242/dev.204289","url":null,"abstract":"Cell labelling and lineage tracing are indispensable tools in developmental biology, offering powerful means to visualise and understand the complex dynamics of cell populations during embryogenesis. Traditional cell labelling relies heavily on signal stability, promoter strength and stage specificity, limiting its application in long-term tracing. In this report, we optimise and reconfigure a perpetual cycling Gal4-UAS system employing a novel Gal4 fusion protein and the autoregulatory Gal4 expression loop. As validated through heat-shock induction, this configuration ensures sustained transcription of reporter genes in target cells and their descendant cells while minimising cytotoxicity, thereby achieving long-term labelling and tracing. Further exploiting this system, we generate zebrafish transgenic lines with continuous fluorescent labelling specific to endoderm, and demonstrate its effectiveness in long-term tracing by showing the progression of endoderm development from embryo to adult, providing visualisation for endodermal cells and their derived tissues. This continuous labelling and tracing strategy can span the entire process of endodermal differentiation from progenitor cells to mature functional cells and is applicable for studying endoderm patterning and organogenesis.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Matrix glycoprotein Papilin maintains the haematopoietic progenitor pool in the Drosophila lymph glands. 基质糖蛋白乳头蛋白维持果蝇淋巴腺中的造血祖细胞池。

IF 3.7 2区生物学

Development Pub Date : 2025-03-17 DOI: 10.1242/dev.204367

Jae-In Lee, Sumin Park, Hyunji Park, Youngbin Lee, JinYoung Park, Donghoon Lee, Moon Jong Kim, Kwang-Min Choe

{"title":"Matrix glycoprotein Papilin maintains the haematopoietic progenitor pool in the Drosophila lymph glands.","authors":"Jae-In Lee, Sumin Park, Hyunji Park, Youngbin Lee, JinYoung Park, Donghoon Lee, Moon Jong Kim, Kwang-Min Choe","doi":"10.1242/dev.204367","DOIUrl":"https://doi.org/10.1242/dev.204367","url":null,"abstract":"Differentiation of prohaemocytes, the precursors of Drosophila blood cells (haemocytes), and the release of haemocytes from the lymph gland, a major larval haematopoietic organ, are vital responses to wasp infestation or tissue degeneration. While cells and extracellular matrices (ECMs) in the lymph gland play a crucial role in haemocyte differentiation, the underlying mechanisms remain unclear. Here, we show that the matrix glycoprotein Papilin (Ppn) is essential for maintaining the prohaemocyte population in lymph glands. In Ppn-depleted larvae, haemocyte differentiation increased with a reduction in the prohaemocyte-containing medullary zone, and lymph gland lobes dispersed prematurely. Ppn was synthesised by plasmatocytes, forming lamellae mainly in the medullary zone. Microbial infection or wasp infestation disrupted the Ppn meshwork within lymph glands. Ppn colocalised with collagen, laminin, nidogen, and perlecan. Ppn depletion disrupted the ECM structure, including perlecan organisation. Phenotypes caused by Ppn depletion were partially rescued by perlecan overexpression or inactivation of the epidermal growth factor receptor (EGFR) pathway. Thus, Ppn is critical in maintaining lymph gland architecture and regulating haemocyte differentiation, highlighting an intricate interaction between ECMs and signalling pathways in haematopoiesis.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143646861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0