Development最新文献_第2页

Mastodon: the command center for large-scale lineage-tracing microscopy datasets. 乳齿象：大规模谱系追踪显微数据集的指挥中心。

IF 4 2区生物学

Development Pub Date : 2026-10-07 DOI: 10.1242/dev.205569

Johannes Girstmair, Tobias Pietzsch, Vladimir Ulman, Stefan Hahmann, Matthias Arzt, Mette Handberg-Thorsager, Samuel Pantze, Ko Sugawara, Robert Haase, Shuichi Onami, Jean-Yves Tinevez, Pavel Tomancak

{"title":"Mastodon: the command center for large-scale lineage-tracing microscopy datasets.","authors":"Johannes Girstmair, Tobias Pietzsch, Vladimir Ulman, Stefan Hahmann, Matthias Arzt, Mette Handberg-Thorsager, Samuel Pantze, Ko Sugawara, Robert Haase, Shuichi Onami, Jean-Yves Tinevez, Pavel Tomancak","doi":"10.1242/dev.205569","DOIUrl":"https://doi.org/10.1242/dev.205569","url":null,"abstract":"Understanding development in living organisms requires following the divisions, movements, and fates of cells. While advances in microscopy have enabled whole-embryo imaging at the cellular level, extracting and analyzing cell lineages from these massive datasets remains a significant computational challenge. We present Mastodon, a scalable, extensible software platform for manual, semi-automated, and automated cell tracking in large images. A purpose-built graph model supports responsive performance for datasets with millions of annotations, making Mastodon a scalable platform for cell lineage analysis. Built as a Fiji plugin, Mastodon enables interactive visualization, editing, and analysis of complex lineage trees, seamlessly integrated with the raw image data. Comprehension of cell lineages in complex three-dimensional geometries is facilitated by interoperability with the powerful open-source render engine Blender. In three distinct developmental contexts, we demonstrate how Mastodon will accelerate biological insights by providing user-friendly navigation and explorative analysis in complex lineage datasets.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149126931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simplified in vitro generation of human gastruloids for modelling early development. 简化体外生成模拟早期发育的人类胃原胃。

IF 4 2区生物学

Development Pub Date : 2026-10-06 DOI: 10.1242/dev.205719

Takuya Azami, E Elizabeth Patton, Jennifer Nichols

{"title":"Simplified in vitro generation of human gastruloids for modelling early development.","authors":"Takuya Azami, E Elizabeth Patton, Jennifer Nichols","doi":"10.1242/dev.205719","DOIUrl":"https://doi.org/10.1242/dev.205719","url":null,"abstract":"The purpose of this study was to optimize the efficiency, cost and reproducibility of human gastruloid formation by testing and adjusting individual parameters using four human pluripotent stem cell lines available from the UK Stem Cell Bank. First, commercially sourced culture medium was replaced with a home-made defined recipe, known as 'N2B27', into which specific reagents can be titrated. By reducing the concentration of Activin A to 15% of the original protocol, efficient elongation of aggregated embryoid bodies was achieved. Also, titrating initial cell density and delaying brief exposure to GSK3 inhibitor until the onset of cell aggregation in individual wells was advantageous. Efficiency of formation of early gastruloids exhibiting the expected regionalization of the three embryonic germ layers was further enhanced by addition of TGFβ-inhibitor. The optimization steps presented thus provide a simplified, robust and relatively economical protocol for consistent generation of elongated gastruloids from human pluripotent stem cells. This streamlined method improves accessibility and reproducibility, also providing an adaptable platform to investigate fundamental principles of early human development.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149105480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The people behind the papers - Ximena Gutiérrez Ramos and Leslie Pick. 这些报纸背后的人，西梅娜·古蒂·萨梅雷斯·拉莫斯和莱斯利·匹克。

IF 4 2区生物学

Development Pub Date : 2026-10-01 Epub Date: 2026-10-07 DOI: 10.1242/dev.206137

引用次数: 0

In preprints - not just a cage: vitelline membranes as scaffolds for tissue morphogenesis. 在预印本中-不仅仅是一个笼子：卵黄膜作为组织形态发生的支架。

IF 4 2区生物学

Development Pub Date : 2026-10-01 Epub Date: 2026-10-08 DOI: 10.1242/dev.206123

Delia Luisa Rodríguez-Bustos, Luis Eduardo Sánchez-Cisneros, Luis Daniel Ríos-Barrera

引用次数: 0

Limb regeneration from amphibians to mammals. 两栖动物到哺乳动物的肢体再生。

IF 4 2区生物学

Development Pub Date : 2026-10-01 Epub Date: 2026-10-08 DOI: 10.1242/dev.205585

Mimi Sammarco, Catherine McCusker

{"title":"Limb regeneration from amphibians to mammals.","authors":"Mimi Sammarco, Catherine McCusker","doi":"10.1242/dev.205585","DOIUrl":"https://doi.org/10.1242/dev.205585","url":null,"abstract":"The ability to regenerate complex appendages varies strikingly across vertebrates, yet the developmental principles that enable complete limb regeneration remain incompletely understood. In mammals, regenerative responses are largely restricted to distal digit tips, whereas amphibians - such as the axolotl - can regenerate entire limbs throughout adulthood. This contrast provides a powerful framework for dissecting how adult tissues rebuild form, pattern and proportion after injury. In this Review, we synthesize recent advances from mammalian digit tip regeneration models and amphibian limb regeneration to examine core challenges of regeneration, including the establishment of regeneration-permissive niches, endogenous reprogramming of adult cells into regeneration-competent progenitors, integration of positional information across limb axes, and regulation of growth and scale. We highlight how comparative and functional studies are revealing conserved developmental programs that support regeneration, as well as key constraints that limit their engagement in mammalian limbs. Rather than serving as a template to be replicated in mammals, the amphibian limb provides a blueprint for understanding whether and how developmental programs can be reactivated (or replaced) in adulthood and why they fail to be fully engaged in mammalian limb injuries.","PeriodicalId":11375,"journal":{"name":"Development","volume":"153 19","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149135273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Blood flow coordinates collective endothelial cell migration during vascular plexus formation and promotes angiogenic sprout regression via flt4. 血流协调血管丛形成过程中内皮细胞的集体迁移，并通过vegfr3/flt4促进血管新生芽的回归。

IF 4 2区生物学

Development Pub Date : 2026-10-01 Epub Date: 2026-09-30 DOI: 10.1242/dev.205424

Yan Chen, Aaron M Savage, Zhen Jiang, Ignacio Rodriguez-Pastrana, Myles Wilson, Hyejeong R Kim, Paul C Evans, Robert N Wilkinson

{"title":"Blood flow coordinates collective endothelial cell migration during vascular plexus formation and promotes angiogenic sprout regression via flt4.","authors":"Yan Chen, Aaron M Savage, Zhen Jiang, Ignacio Rodriguez-Pastrana, Myles Wilson, Hyejeong R Kim, Paul C Evans, Robert N Wilkinson","doi":"10.1242/dev.205424","DOIUrl":"10.1242/dev.205424","url":null,"abstract":"Nascent vascular networks adapt to the increasing metabolic demands of growing tissues by expanding via angiogenesis. As vascular networks expand, blood vessels remodel, progressively refining vascular connectivity to generate a more haemodynamically efficient network. This is driven by interplay between endothelial cell (EC) signalling and blood flow. While much is known about angiogenesis, less is understood of how blood flow remodels vessels. Here, we employ the zebrafish sub-intestinal venous plexus (SIVP) to characterise blood flow-dependent remodelling. Using live imaging to track ECs, we show that blood flow controls SIVP remodelling by coordinating collective EC migration within the developing plexus. Blood flow opposes continuous ventral EC migration within the SIVP and is required for angiogenic sprout regression to support plexus growth. Sprout regression occurs by coordinated migration of ECs from non-perfused leading sprouts, which migrate against blood flow and incorporate into the sub-intestinal vein. Sprout regression remains compatible with low blood flow but requires flt4 function under these conditions. Collectively, these studies reveal how blood flow sculpts a developing vascular plexus by coordinating EC migration and balancing vascular remodelling via flt4.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148900097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In preprints - reading a cell in two tenses: the proteome holds the present, the transcriptome points to the future. 在预印本中——用两种时态解读细胞：蛋白质组掌握现在，转录组指向未来。

IF 4 2区生物学

Development Pub Date : 2026-10-01 DOI: 10.1242/dev.206111

Roshana Thambyrajah

引用次数: 0

Modular presynaptic assemblages scale to postsynaptic partner number. 模块化突触前组装规模突触后伙伴数。

IF 4 2区生物学

Development Pub Date : 2026-10-01 Epub Date: 2026-10-09 DOI: 10.1242/dev.205453

Vanessa M Puñal, Valentina Caicedo-Garzón, Emma M Thornton-Kolbe, Jasmine Dhillon, Jackson A Rogow, E Josephine Clowney

{"title":"Modular presynaptic assemblages scale to postsynaptic partner number.","authors":"Vanessa M Puñal, Valentina Caicedo-Garzón, Emma M Thornton-Kolbe, Jasmine Dhillon, Jackson A Rogow, E Josephine Clowney","doi":"10.1242/dev.205453","DOIUrl":"10.1242/dev.205453","url":null,"abstract":"Behavioral diversification can arise through evolutionary and inter-individual differences in neural circuit development. In the mushroom body, which is an associative learning center of arthropods, the number of Kenyon cells (KCs) varies across species and among individuals. How such variation is developmentally accommodated by projection neurons (PNs), which provide sensory input to KCs, is not understood. In Drosophila melanogaster, we have previously demonstrated that PNs scale their presynaptic bouton number to KC population size. Here, we identify developmental mechanisms underlying this input flexibility. Boutons form on axonal collaterals; for PNs, we find that collateral number is subtype specific and serves as a substrate through which bouton number scales to KC population size. Independent of PN identity or KC number, most individual collaterals produce only one to three boutons, suggesting collaterals are modular cell biological bouton units. Developing PNs initially overproduce nascent collaterals in the early pupa. The set of nascent collaterals that form boutons and mature is conditional on KC number, thereby executing scaling. Finally, early boutons bear filopodia that contact neighboring PN processes, suggesting that bouton-bouton interactions contribute to shaping these structures.","PeriodicalId":11375,"journal":{"name":"Development","volume":"153 19","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149147080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Postnatal heart development is coordinated by multi-lineage Wnt and BMP signaling. 出生后心脏发育是由多谱系Wnt和BMP信号协调的。

IF 4 2区生物学

Development Pub Date : 2026-10-01 Epub Date: 2026-09-29 DOI: 10.1242/dev.205448

Hui An, Hao Hu, Saijing Chen, Tingting Chen, Yafei Hu, Tongtong Yu, Anlan Wang, Shengwei Jin, Ming Li

{"title":"Postnatal heart development is coordinated by multi-lineage Wnt and BMP signaling.","authors":"Hui An, Hao Hu, Saijing Chen, Tingting Chen, Yafei Hu, Tongtong Yu, Anlan Wang, Shengwei Jin, Ming Li","doi":"10.1242/dev.205448","DOIUrl":"10.1242/dev.205448","url":null,"abstract":"Early postnatal heart growth requires a transition from cardiomyocyte proliferation to enlargement and binucleation. Using morphometric, transcriptomic, signaling and single-cell analyses in neonatal mice, we show that this transition occurs predominantly between postnatal days 3.5 and 4.5 (P3.5-P4.5), accompanied by declining β-catenin abundance and increased SMAD1/5/9 phosphorylation. Pharmacological disruption of β-catenin/CBP signaling or Ctnnb1 knockdown promotes cardiomyocyte enlargement, whereas β-catenin stabilization by CHIR-99021 enhances cell-cycle activity and estimated ventricular cardiomyocyte number while limiting enlargement. Transcriptomic analysis reveals a Sox2/Zic1/Olig2 module enriched in early neural/glial cells, suggesting the onset of postnatal cardiac nervous system development. Wnt pathway components are active in endothelial, mesenchymal and neural populations during the early postnatal window, whereas BMP signaling peaks in cardiomyocytes at P6. These findings reveal a multi-lineage interplay between Wnt and BMP signaling that balances cardiomyocyte number expansion versus enlargement during early postnatal heart development.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149037079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In preprints - making blood stem cells: how close is close enough? 在预印本制造血液干细胞：多接近才算足够接近？

IF 4 2区生物学

Development Pub Date : 2026-10-01 Epub Date: 2026-09-30 DOI: 10.1242/dev.206125

Roshana Thambyrajah

引用次数: 0