Development最新文献_第2页

In preprints: exploring developmental robustness and timing with gastruloids. 预印本：探索类胃原体的发育稳健性和时间。

IF 3.7 2区生物学

Development Pub Date : 2025-05-15 DOI: 10.1242/dev.204870

Yonit Maroudas-Sacks, Marc Trani Bustos, Jesse V Veenvliet

引用次数: 0

A function of Spalt proteins in heterochromatin organization and maintenance of genomic DNA integrity. Spalt蛋白在异染色质组织和维持基因组DNA完整性中的功能。

IF 3.7 2区生物学

Development Pub Date : 2025-05-15 Epub Date: 2025-05-16 DOI: 10.1242/dev.204258

Cristina M Ostalé, Natalia Azpiazu, Ana Peropadre, Mercedes Martín, Mireya Ruiz-Losada, Ana López-Varea, Rebecca R Viales, Charles Girardot, Eileen E M Furlong, Jose F de Celis

{"title":"A function of Spalt proteins in heterochromatin organization and maintenance of genomic DNA integrity.","authors":"Cristina M Ostalé, Natalia Azpiazu, Ana Peropadre, Mercedes Martín, Mireya Ruiz-Losada, Ana López-Varea, Rebecca R Viales, Charles Girardot, Eileen E M Furlong, Jose F de Celis","doi":"10.1242/dev.204258","DOIUrl":"10.1242/dev.204258","url":null,"abstract":"The conserved Spalt proteins regulate gene expression and cell fate choices during multicellular development, generally acting as transcriptional repressors in different gene regulatory networks. In addition to their roles as DNA sequence-specific transcription factors, Spalt proteins show a consistent localization to heterochromatic regions. Vertebrate Spalt-like proteins can act through the nucleosome remodeling and deacetylase complex to promote closing of open chromatin domains, but their activities also rely on interactions with DNA methyltransferases or with the lysine-specific histone demethylase LSD1, suggesting that they participate in multiple regulatory mechanisms. Here, we describe several consequences of loss of Spalt function in Drosophila cells, including changes in chromatin accessibility, generation of DNA damage, alterations in the localization of chromosomes within the nucleus in the salivary glands and misexpression of transposable elements. We suggest that these effects are related to roles of Spalt proteins in the regulation of heterochromatin formation and chromatin organization. We propose that Drosophila Spalt proteins have two complementary functions, acting as sequence-specific transcriptional repressors on specific target genes and regulating more global gene silencing through the generation or maintenance of heterochromatic domains.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12091872/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143989513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Measuring and manipulating localized translation of erm-1 in the C. elegans embryo. 线虫胚胎中erm-1的定位翻译的测量和操作。

IF 3.7 2区生物学

Development Pub Date : 2025-05-15 Epub Date: 2025-05-19 DOI: 10.1242/dev.204435

Elise van der Salm, Esther Koelewijn, Mette Schroeder, Erica van der Maas, Olga Jarosińska, Max Eeken, Suzan Ruijtenberg

{"title":"Measuring and manipulating localized translation of erm-1 in the C. elegans embryo.","authors":"Elise van der Salm, Esther Koelewijn, Mette Schroeder, Erica van der Maas, Olga Jarosińska, Max Eeken, Suzan Ruijtenberg","doi":"10.1242/dev.204435","DOIUrl":"10.1242/dev.204435","url":null,"abstract":"Translation of mRNAs into proteins is key in decoding the information stored in the genome. Localized translation ensures that proteins are expressed where needed, which is important for cell-specific protein expression, the establishment of cellular protein gradients and the creation of protein hotspots. Although localized translation is believed to be important for cell fate determination and organismal development, our understanding of localized translation in the context of living animals is limited, as few methods exist that allow direct visualization and measurement of translation. We adapted the SunTag-based single-molecule translation imaging system for use in Caenorhabditis elegans, and show the dynamics and importance of localized erm-1 translation during development. We found erm-1 translation to be enriched at the plasma membrane, overlapping with the localization and function of the encoded membrane-cytoskeleton linker ERM-1. Re-localizing erm-1 translation to nuclear pores disrupts the function of ERM-1 protein, particularly its role in linking the actin cytoskeleton to the membrane, leading to defects in intestinal lumen formation. Our work demonstrates the power of translation imaging and highlights the importance of localized translation in C. elegans development.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143983316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The people behind the papers - Yan Wu, Yiling Lan and Heidi Hehnly. 这些报纸背后的人——吴燕、兰一玲和海蒂。

IF 3.7 2区生物学

Development Pub Date : 2025-05-15 Epub Date: 2025-05-19 DOI: 10.1242/dev.204888

引用次数: 0

In preprints: are microbes the architects of animal bodies? 预印本：微生物是动物身体的建筑师吗？

IF 3.7 2区生物学

Development Pub Date : 2025-05-15 Epub Date: 2025-05-14 DOI: 10.1242/dev.204884

Alessandro Bonfini, Peter Nagy, Nicolas Buchon

引用次数: 0

Evidence of secondary Notch signaling within the rat small intestine. 大鼠小肠内二次Notch信号的证据。

IF 3.7 2区生物学

Development Pub Date : 2025-05-15 DOI: 10.1242/dev.204277

Eleanor Zagoren, Nicolas Dias, Anderson K Santos, Zachary D Smith, Nadia A Ameen, Kaelyn Sumigray

{"title":"Evidence of secondary Notch signaling within the rat small intestine.","authors":"Eleanor Zagoren, Nicolas Dias, Anderson K Santos, Zachary D Smith, Nadia A Ameen, Kaelyn Sumigray","doi":"10.1242/dev.204277","DOIUrl":"https://doi.org/10.1242/dev.204277","url":null,"abstract":"The small intestine is well known for its nutrient-absorbing enterocytes; yet equally critical for homeostasis is a diverse set of secretory cells, all presumed to originate from the same intestinal stem cell. Despite their major roles in intestinal function and health, understanding how the full spectrum of secretory cell types arises remains a longstanding challenge, largely due to their comparative rarity. Here, we investigate the specification of a rare population of small intestinal epithelial cells found in rats and humans but not mice: CFTR High Expressers (CHEs). Using pseudotime trajectory analysis of single-cell RNA-seq data from rat jejunum, we provide evidence that CHEs are specified along the secretory lineage and appear to employ a second wave of Notch-based signaling to distinguish themselves from other secretory cells. We validate the transcription factors directing these cells from crypt progenitors and demonstrate that Notch signaling is necessary to induce CHE fate in vivo and in vitro. Our findings suggest that Notch reactivation along the secretory lineage specifies CHEs, which may help regulate luminal pH and have direct relevance in cystic fibrosis pathophysiology.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144076655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

FD and FDP bZIP transcription factors and FT florigen regulate floral development and control homeotic gene expression in Arabidopsis floral meristems. FD/FDP bZIP转录因子和FT花原调控拟南芥花分生组织的发育和同源基因表达。

IF 3.7 2区生物学

Development Pub Date : 2025-05-15 Epub Date: 2025-05-22 DOI: 10.1242/dev.204241

Maida Romera-Branchat, Chloé Pocard, Coral Vincent, Martina Cerise, Vítor da Silveira Falavigna, Alice Pajoro, Na Ding, He Gao, Rainer Franzen, George Coupland

{"title":"FD and FDP bZIP transcription factors and FT florigen regulate floral development and control homeotic gene expression in Arabidopsis floral meristems.","authors":"Maida Romera-Branchat, Chloé Pocard, Coral Vincent, Martina Cerise, Vítor da Silveira Falavigna, Alice Pajoro, Na Ding, He Gao, Rainer Franzen, George Coupland","doi":"10.1242/dev.204241","DOIUrl":"10.1242/dev.204241","url":null,"abstract":"Arabidopsis florigen activation complex (FAC), formed by the interaction of the transcription factor FD and the florigen protein FT, activates gene expression in the shoot apical meristem to induce flowering. We show that FD and its paralog FDP are also expressed in partially overlapping patterns in the floral meristem and floral organs, and that FT is present in floral meristems. The flowers of mutants for FT and its paralog TSF (ft tsf), and of fd fdp mutants show variable numbers of sepals and petals, and larger floral meristems than wild type. In the floral meristem, fd fdp and ft tsf mutants show a significant reduction in the expression of SEP and AG genes, which encode MADS-domain transcription factors, as well as increased expression of the homeobox gene WUS. Binding of FD to SEP genes suggests that diminished SEP gene expression is a primary defect in the mutants. We conclude that, beyond their role in floral transition, FAC components regulate floral homeotic gene expression to control floral meristem size, and influence floral organ development and identity.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143977784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chromatin profiling data indicate regulatory mechanisms for differentiation during development in the acoel Hofstenia miamia. 染色质谱分析数据表明了迈阿密霍夫斯氏菌在发育过程中分化的调控机制。

IF 3.7 2区生物学

Development Pub Date : 2025-05-15 Epub Date: 2025-05-23 DOI: 10.1242/dev.204799

Paul Bump, Kaitlyn Loubet-Senear, Sarah Arnold, Mansi Srivastava

{"title":"Chromatin profiling data indicate regulatory mechanisms for differentiation during development in the acoel Hofstenia miamia.","authors":"Paul Bump, Kaitlyn Loubet-Senear, Sarah Arnold, Mansi Srivastava","doi":"10.1242/dev.204799","DOIUrl":"10.1242/dev.204799","url":null,"abstract":"Chromatin profiling data can generate and corroborate hypotheses for regulatory events that underlie the control of gene expression in biological processes. Here, we have profiled chromatin accessibility to build a catalog of putative regulatory DNA during embryonic development in an acoel. Acoels represent an enigmatic phylum-level lineage of animals, the Xenacoelomorpha, which is placed either as a sister group to all other animals with bilateral symmetry or as an early diverging ambulacrarian, positioned equally well to inform the evolution of developmental mechanisms. We focused on the acoel Hofstenia miamia, a new research organism for studying whole-body regeneration that also enables investigations of development from zygote to hatching. We profiled chromatin landscapes encompassing major morphological events during development, and combined transcription factor-binding analyses with single-cell RNA-sequencing data to provide regulatory linkages in a hypothesized differentiation trajectory for epidermis, as well as a new gene regulatory network associated with the formation of muscle. This work enables comparisons of chromatin state during embryogenesis between acoels and other animals, as well as comparisons of embryogenesis to regeneration.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143997366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Developmental dynamics of marmoset prefrontal cortical SST and PV interneuron networks highlight primate-specific features. 前额皮质SST和PV中间神经元网络的发育动力学：来自猴子的见解突出了人类特有的特征。

IF 3.7 2区生物学

Development Pub Date : 2025-05-15 Epub Date: 2025-05-19 DOI: 10.1242/dev.204254

Nafiseh S Hosseini Fin, Adrian Yip, Jack T Scott, Leon Teo, Jihane Homman-Ludiye, James A Bourne

{"title":"Developmental dynamics of marmoset prefrontal cortical SST and PV interneuron networks highlight primate-specific features.","authors":"Nafiseh S Hosseini Fin, Adrian Yip, Jack T Scott, Leon Teo, Jihane Homman-Ludiye, James A Bourne","doi":"10.1242/dev.204254","DOIUrl":"10.1242/dev.204254","url":null,"abstract":"The primate prefrontal cortex (PFC) undergoes protracted postnatal development, crucial for the emergence of cognitive control and executive function. Central to this maturation are inhibitory interneurons (INs), particularly parvalbumin-expressing (PV+) and somatostatin-expressing (SST+) subtypes, which regulate cortical circuit timing and plasticity. While rodent models have provided foundational insights into IN development, the trajectory of postmigratory maturation in primates remains largely uncharted. In this study, we characterized the expression of PV, SST, the chloride transporter KCC2, and the ion channels Kv3.1b and Nav1.1 across six PFC regions (areas 8aD, 8aV, 9, 46, 11 and 47L) in the postnatal marmoset. We report a prolonged maturation of PV+ INs into adolescence, accompanied by progressive upregulation of ion channels that support high-frequency firing. In contrast, SST+ INs show a postnatal decline in density, diverging from rodent developmental patterns. These findings reveal distinct, cell type-specific maturation dynamics in the primate PFC and offer a developmental framework for understanding how inhibitory circuit refinement may underlie vulnerability to neurodevelopmental disorders.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143988233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Condensin II complex regulates essential gene expression programs during erythropoiesis. 凝血素II复合体调节红细胞生成过程中必需基因表达程序的表达。

IF 3.7 2区生物学

Development Pub Date : 2025-05-15 Epub Date: 2025-05-19 DOI: 10.1242/dev.204485

Deanna Abid, Kristin Murphy, Zachary Murphy, Nabil Rahman, Michael Getman, Laurie Steiner

{"title":"The Condensin II complex regulates essential gene expression programs during erythropoiesis.","authors":"Deanna Abid, Kristin Murphy, Zachary Murphy, Nabil Rahman, Michael Getman, Laurie Steiner","doi":"10.1242/dev.204485","DOIUrl":"10.1242/dev.204485","url":null,"abstract":"Erythropoiesis is characterized by dramatic changes in gene expression in the context of a cell that is rapidly proliferating while simultaneously condensing its nucleus in anticipation of enucleation. The mechanisms that maintain high level expression of erythroid genes and promote nuclear condensation remain poorly understood. Condensin II is a ring-like complex that promotes mitotic chromatin condensation and has roles in regulating interphase chromatin architecture and gene expression. We interrogated the role of Condensin II in erythropoiesis using an erythroid-specific deletion of the Condensin II subunit, Ncaph2. Ncaph2 loss resulted in severe anemia by embryonic day 12.5 with embryonic lethality. Ncaph2 mutant erythroid cells had dysregulated maturation and disrupted cell cycle progression, but surprisingly NCAPH2 was dispensable for nuclear condensation. Genomic studies revealed that NCAPH2 occupied the promoter of key erythroid and cell cycle genes that were downregulated following Ncaph2 loss. Together, our results demonstrate an essential role for NCAPH2 in the gene expression programs that regulate cell cycle progression and erythroid differentiation, and identify a role for the Condensin II complex in the regulation of a lineage-specific differentiation program.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143988011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0