Development最新文献_第2页

Interactions between U and V sex chromosomes during the life cycle of Ectocarpus. 外果藻生命周期中 U 和 V 性染色体之间的相互作用

IF 4.6 2区生物学

Development Pub Date : 2024-10-15 Epub Date: 2024-04-11 DOI: 10.1242/dev.202677

Jeromine Vigneau, Claudia Martinho, Olivier Godfroy, Min Zheng, Fabian B Haas, Michael Borg, Susana M Coelho

{"title":"Interactions between U and V sex chromosomes during the life cycle of Ectocarpus.","authors":"Jeromine Vigneau, Claudia Martinho, Olivier Godfroy, Min Zheng, Fabian B Haas, Michael Borg, Susana M Coelho","doi":"10.1242/dev.202677","DOIUrl":"10.1242/dev.202677","url":null,"abstract":"In many animals and flowering plants, sex determination occurs in the diploid phase of the life cycle with XX/XY or ZW/ZZ sex chromosomes. However, in early diverging plants and most macroalgae, sex is determined by female (U) or male (V) sex chromosomes in a haploid phase called the gametophyte. Once the U and V chromosomes unite at fertilization to produce a diploid sporophyte, sex determination no longer occurs, raising key questions about the fate of the U and V sex chromosomes in the sporophyte phase. Here, we investigate genetic and molecular interactions of the UV sex chromosomes in both the haploid and diploid phases of the brown alga Ectocarpus. We reveal extensive developmental regulation of sex chromosome genes across its life cycle and implicate the TALE-HD transcription factor OUROBOROS in suppressing sex determination in the diploid phase. Small RNAs may also play a role in the repression of a female sex-linked gene, and transition to the diploid sporophyte coincides with major reconfiguration of histone H3K79me2, suggesting a more intricate role for this histone mark in Ectocarpus development than previously appreciated.","PeriodicalId":11375,"journal":{"name":"Development","volume":null,"pages":null},"PeriodicalIF":4.6,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11057875/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140184036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Variations in cell plasticity and proliferation underlie distinct modes of regeneration along the antero-posterior axis in the annelid Platynereis. 细胞可塑性和增殖的变化是环带蝶类（Platynereis）沿前后轴再生的不同模式的基础。

IF 3.7 2区生物学

Development Pub Date : 2024-10-15 Epub Date: 2024-07-02 DOI: 10.1242/dev.202452

Loïc Bideau, Zoé Velasquillo-Ramirez, Loeiza Baduel, Marianne Basso, Pascale Gilardi-Hebenstreit, Vanessa Ribes, Michel Vervoort, Eve Gazave

{"title":"Variations in cell plasticity and proliferation underlie distinct modes of regeneration along the antero-posterior axis in the annelid Platynereis.","authors":"Loïc Bideau, Zoé Velasquillo-Ramirez, Loeiza Baduel, Marianne Basso, Pascale Gilardi-Hebenstreit, Vanessa Ribes, Michel Vervoort, Eve Gazave","doi":"10.1242/dev.202452","DOIUrl":"https://doi.org/10.1242/dev.202452","url":null,"abstract":"The capacity to regenerate lost tissues varies significantly among animals. Some phyla, such as the annelids, display substantial regenerating abilities, although little is known about the cellular mechanisms underlying the process. To precisely determine the origin, plasticity and fate of the cells participating in blastema formation and posterior end regeneration after amputation in the annelid Platynereis dumerilii, we developed specific tools to track different cell populations. Using these tools, we find that regeneration is partly promoted by a population of proliferative gut cells whose regenerative potential varies as a function of their position along the antero-posterior axis of the worm. Gut progenitors from anterior differentiated tissues are lineage restricted, whereas gut progenitors from the less differentiated and more proliferative posterior tissues are much more plastic. However, they are unable to regenerate the stem cells responsible for the growth of the worms. Those stem cells are of local origin, deriving from the cells present in the segment abutting the amputation plane, as are most of the blastema cells. Our results favour a hybrid and flexible cellular model for posterior regeneration in Platynereis relying on different degrees of cell plasticity.","PeriodicalId":11375,"journal":{"name":"Development","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141476187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A change in cis-regulatory logic underlying obligate versus facultative muscle multinucleation in chordates. 脊索动物肌肉多核形成的顺式调控逻辑变化

IF 3.7 2区生物学

Development Pub Date : 2024-10-15 Epub Date: 2024-09-03 DOI: 10.1242/dev.202968

Christopher J Johnson, Zheng Zhang, Haifeng Zhang, Renjie Shang, Katarzyna M Piekarz, Pengpeng Bi, Alberto Stolfi

{"title":"A change in cis-regulatory logic underlying obligate versus facultative muscle multinucleation in chordates.","authors":"Christopher J Johnson, Zheng Zhang, Haifeng Zhang, Renjie Shang, Katarzyna M Piekarz, Pengpeng Bi, Alberto Stolfi","doi":"10.1242/dev.202968","DOIUrl":"10.1242/dev.202968","url":null,"abstract":"Vertebrates and tunicates are sister groups that share a common fusogenic factor, Myomaker (Mymk), that drives myoblast fusion and muscle multinucleation. Yet they are divergent in when and where they express Mymk. In vertebrates, all developing skeletal muscles express Mymk and are obligately multinucleated. In tunicates, Mymk is expressed only in post-metamorphic multinucleated muscles, but is absent from mononucleated larval muscles. In this study, we demonstrate that cis-regulatory sequence differences in the promoter region of Mymk underlie the different spatiotemporal patterns of its transcriptional activation in tunicates and vertebrates. Although in vertebrates myogenic regulatory factors (MRFs) such as MyoD1 alone are required and sufficient for Mymk transcription in all skeletal muscles, we show that transcription of Mymk in post-metamorphic muscles of the tunicate Ciona requires the combinatorial activity of MRF, MyoD and Early B-cell Factor (Ebf). This macroevolutionary difference appears to be encoded in cis, likely due to the presence of a putative Ebf-binding site adjacent to predicted MRF binding sites in the Ciona Mymk promoter. We further discuss how Mymk and myoblast fusion might have been regulated in the last common ancestor of tunicates and vertebrates, for which we propose two models.","PeriodicalId":11375,"journal":{"name":"Development","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11441980/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141901235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Uncovering developmental diversity in the field. 发现实地发展的多样性。

IF 3.7 2区生物学

Development Pub Date : 2024-10-15 Epub Date: 2024-08-19 DOI: 10.1242/dev.203084

Federico D Brown, Edson Ishengoma, Georg Mayer, Natalia Pabón-Mora, M Emília Santos, Karen E Sears, Ivo de Sena Oliveira

引用次数: 0

MUM, a maternal unknown message, inhibits early establishment of the medio-lateral axis in the embryo of the kelp Saccharina latissima. 母体未知信息 MUM 可抑制海带 Saccharina latissima 胚胎中轴线的早期形成。

IF 3.7 2区生物学

Development Pub Date : 2024-10-15 Epub Date: 2024-09-13 DOI: 10.1242/dev.202732

Samuel Boscq, Bernard Billoud, Ioannis Theodorou, Tanweer Joemmanbaks, Tanguy Dufourt, Bénédicte Charrier

{"title":"MUM, a maternal unknown message, inhibits early establishment of the medio-lateral axis in the embryo of the kelp Saccharina latissima.","authors":"Samuel Boscq, Bernard Billoud, Ioannis Theodorou, Tanweer Joemmanbaks, Tanguy Dufourt, Bénédicte Charrier","doi":"10.1242/dev.202732","DOIUrl":"10.1242/dev.202732","url":null,"abstract":"Brown algae are multicellular photosynthetic organisms that have evolved independently of plants and other algae. Here, we have studied the determinism of body axis formation in the kelp Saccharina latissima. After microdissection of the embryo, we show that the stalk, an empty cell that retains the embryo on the maternal tissue, represses longitudinal cell divisions in the early embryo, thereby reinforcing the establishment of the initial apico-basal axis. In addition, it promotes cell growth and controls cell shape and arrangement in the flat oblong embryo composed of cells aligned in rows and columns. Although the stalk persists for several weeks until the embryo reaches at least 500 cells, proper embryogenesis requires connection to maternal tissue only during the first 4 days after fertilisation, i.e. before the embryo reaches the 8-cell stage. Transplantation experiments indicate that the maternal signal is not diffused in seawater, but requires contact between the embryo and the maternal tissue. This first global quantitative study of brown algal embryogenesis highlights the role of MUM, an unknown maternal message, in the control of growth axes and tissue patterning in kelp embryos.","PeriodicalId":11375,"journal":{"name":"Development","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11423915/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142072343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A pre-vertebrate endodermal origin of calcitonin-producing neuroendocrine cells. 前脊椎动物内胚层降钙素神经内分泌细胞的起源。

IF 3.7 2区生物学

Development Pub Date : 2024-10-15 Epub Date: 2024-08-07 DOI: 10.1242/dev.202821

Jenaid M Rees, Katie Kirk, Giacomo Gattoni, Dorit Hockman, Victoria A Sleight, Dylan J Ritter, Èlia Benito-Gutierrez, Ela W Knapik, J Gage Crump, Peter Fabian, J Andrew Gillis

{"title":"A pre-vertebrate endodermal origin of calcitonin-producing neuroendocrine cells.","authors":"Jenaid M Rees, Katie Kirk, Giacomo Gattoni, Dorit Hockman, Victoria A Sleight, Dylan J Ritter, Èlia Benito-Gutierrez, Ela W Knapik, J Gage Crump, Peter Fabian, J Andrew Gillis","doi":"10.1242/dev.202821","DOIUrl":"10.1242/dev.202821","url":null,"abstract":"Vertebrate calcitonin-producing cells (C-cells) are neuroendocrine cells that secrete the small peptide hormone calcitonin in response to elevated blood calcium levels. Whereas mouse C-cells reside within the thyroid gland and derive from pharyngeal endoderm, avian C-cells are located within ultimobranchial glands and have been reported to derive from the neural crest. We use a comparative cell lineage tracing approach in a range of vertebrate model systems to resolve the ancestral embryonic origin of vertebrate C-cells. We find, contrary to previous studies, that chick C-cells derive from pharyngeal endoderm, with neural crest-derived cells instead contributing to connective tissue intimately associated with C-cells in the ultimobranchial gland. This endodermal origin of C-cells is conserved in a ray-finned bony fish (zebrafish) and a cartilaginous fish (the little skate, Leucoraja erinacea). Furthermore, we discover putative C-cell homologs within the endodermally-derived pharyngeal epithelium of the ascidian Ciona intestinalis and the amphioxus Branchiostoma lanceolatum, two invertebrate chordates that lack neural crest cells. Our findings point to a conserved endodermal origin of C-cells across vertebrates and to a pre-vertebrate origin of this cell type along the chordate stem.","PeriodicalId":11375,"journal":{"name":"Development","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141897072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stable germline transgenesis using the Minos Tc1/mariner element in the sea urchin Lytechinus pictus. 在海胆（Lytechinus pictus）中使用米诺斯 Tc1/mariner 基因进行稳定的种系转基因。

IF 3.7 2区生物学

Development Pub Date : 2024-10-15 Epub Date: 2024-08-19 DOI: 10.1242/dev.202991

Elliot W Jackson, Emilio Romero, Svenja Kling, Yoon Lee, Evan Tjeerdema, Amro Hamdoun

{"title":"Stable germline transgenesis using the Minos Tc1/mariner element in the sea urchin Lytechinus pictus.","authors":"Elliot W Jackson, Emilio Romero, Svenja Kling, Yoon Lee, Evan Tjeerdema, Amro Hamdoun","doi":"10.1242/dev.202991","DOIUrl":"10.1242/dev.202991","url":null,"abstract":"Stable transgenesis is a transformative tool in model organism biology. Although the sea urchin is one of the oldest animal models in cell and developmental biology, studies in this animal have largely relied on transient manipulation of wild animals, without a strategy for stable transgenesis. Here, we build on recent progress to develop a more genetically tractable sea urchin species, Lytechinus pictus, and establish a robust transgene integration method. Three commonly used transposons (Minos, Tol2 and piggyBac) were tested for non-autonomous transposition, using plasmids containing a polyubiquitin promoter upstream of a H2B-mCerulean nuclear marker. Minos was the only transposable element that resulted in significant expression beyond metamorphosis. F0 animals were raised to sexual maturity, and spawned to determine germline integration and transgene inheritance frequency, and to characterize expression patterns of the transgene in F1 progeny. The results demonstrate transgene transmission through the germline, the first example of a germline transgenic sea urchin and, indeed, of any echinoderm. This milestone paves the way for the generation of diverse transgenic resources that will dramatically enhance the utility, reproducibility and efficiency of sea urchin research.","PeriodicalId":11375,"journal":{"name":"Development","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11361634/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141632962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

VitelloTag: a tool for high-throughput cargo delivery into oocytes. VitelloTag：将货物高通量送入卵母细胞的工具。

IF 3.7 2区生物学

Development Pub Date : 2024-10-15 Epub Date: 2024-09-14 DOI: 10.1242/dev.202857

D Nathaniel Clarke, Akshay Kane, Margherita Perillo, Christopher J Lowe, S Zachary Swartz

引用次数: 0

Regulation of ROP GTPase cycling between active and inactive states is essential for vegetative organogenesis in Marchantia polymorpha. 调节 ROP GTPase 在活性/非活性状态之间的循环对多瘤马钱子无性生殖器官的形成至关重要。

IF 3.7 2区生物学

Development Pub Date : 2024-10-15 Epub Date: 2024-09-10 DOI: 10.1242/dev.202928

Yuuki Sakai, Aki Ueno, Hiroki Yonetsuka, Tatsuaki Goh, Hirotaka Kato, Yuki Kondo, Hidehiro Fukaki, Kimitsune Ishizaki

{"title":"Regulation of ROP GTPase cycling between active and inactive states is essential for vegetative organogenesis in Marchantia polymorpha.","authors":"Yuuki Sakai, Aki Ueno, Hiroki Yonetsuka, Tatsuaki Goh, Hirotaka Kato, Yuki Kondo, Hidehiro Fukaki, Kimitsune Ishizaki","doi":"10.1242/dev.202928","DOIUrl":"10.1242/dev.202928","url":null,"abstract":"Rho/Rac of plant (ROP) GTPases are plant-specific proteins that function as molecular switches, activated by guanine nucleotide exchange factors (GEFs) and inactivated by GTPase-activating proteins (GAPs). The bryophyte Marchantia polymorpha contains single copies of ROP (MpROP), GEFs [ROPGEF and SPIKE (SPK)] and GAPs [ROPGAP and ROP ENHANCER (REN)]. MpROP regulates the development of various tissues and organs, such as rhizoids, gemmae and air chambers. The ROPGEF KARAPPO (MpKAR) is essential for gemma initiation, but the functions of other ROP regulatory factors are less understood. This study focused on two GAPs: MpROPGAP and MpREN. Mpren single mutants showed defects in thallus growth, rhizoid tip growth, gemma development, and air-chamber formation, whereas Mpropgap mutants showed no visible abnormalities. However, Mpropgap Mpren double mutants had more severe phenotypes than the Mpren single mutants, suggesting backup roles of MpROPGAP in processes involving MpREN. Overexpression of MpROPGAP and MpREN resulted in similar gametophyte defects, highlighting the importance of MpROP activation/inactivation cycling (or balancing). Thus, MpREN predominantly, and MpROPGAP as a backup, regulate gametophyte development, likely by controlling MpROP activation in M. polymorpha.","PeriodicalId":11375,"journal":{"name":"Development","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141916323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of auxin responses in the fern Ceratopteris richardii identifies the developmental phase as a major determinant for response properties. 对蕨类植物 Ceratopteris richardii 的辅助剂反应分析表明，发育阶段是反应特性的主要决定因素。

IF 3.7 2区生物学

Development Pub Date : 2024-10-15 Epub Date: 2024-09-26 DOI: 10.1242/dev.203026

Sjoerd Woudenberg, Melissa Dipp Alvarez, Juriaan Rienstra, Victor Levitsky, Victoria Mironova, Enrico Scarpella, Andre Kuhn, Dolf Weijers

{"title":"Analysis of auxin responses in the fern Ceratopteris richardii identifies the developmental phase as a major determinant for response properties.","authors":"Sjoerd Woudenberg, Melissa Dipp Alvarez, Juriaan Rienstra, Victor Levitsky, Victoria Mironova, Enrico Scarpella, Andre Kuhn, Dolf Weijers","doi":"10.1242/dev.203026","DOIUrl":"10.1242/dev.203026","url":null,"abstract":"The auxin signaling molecule regulates a range of plant growth and developmental processes. The core transcriptional machinery responsible for auxin-mediated responses is conserved across all land plants. Genetic, physiological and molecular exploration in bryophyte and angiosperm model species have shown both qualitative and quantitative differences in auxin responses. Given the highly divergent ontogeny of the dominant gametophyte (bryophytes) and sporophyte (angiosperms) generations, however, it is unclear whether such differences derive from distinct phylogeny or ontogeny. Here, we address this question by comparing a range of physiological, developmental and molecular responses to auxin in both generations of the model fern Ceratopteris richardii. We find that auxin response in Ceratopteris gametophytes closely resembles that of a thalloid bryophyte, whereas the sporophyte mimics auxin response in flowering plants. This resemblance manifests both at the phenotypic and transcriptional levels. Furthermore, we show that disrupting auxin transport can lead to ectopic sporophyte induction on the gametophyte, suggesting a role for auxin in the alternation of generations. Our study thus identifies developmental phase, rather than phylogeny, as a major determinant of auxin response properties in land plants.","PeriodicalId":11375,"journal":{"name":"Development","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11449451/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142343727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0