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The people behind the papers - Roberta Azzarelli and Anna Philpott.
IF 3.7 2区 生物学
Development Pub Date : 2024-12-15 Epub Date: 2024-12-12 DOI: 10.1242/dev.204575
{"title":"The people behind the papers - Roberta Azzarelli and Anna Philpott.","authors":"","doi":"10.1242/dev.204575","DOIUrl":"https://doi.org/10.1242/dev.204575","url":null,"abstract":"<p><p>ASCL1 is a pioneer factor that can reprogram somatic cells to produce neurons. Preventing ASCL1 from being phosphorylated appears to enhance its reprogramming abilities, but the reason for this is unclear. A new paper in Development explores how ASCL1 activity is affected by different cellular contexts and reveals that the basis of the reprogramming efficiency of ASCL1 is more complicated than it first appears. To learn more about the story behind the paper, we caught up with first author Roberta Azzarelli, who is now a Lecturer in Pharmacology at University College London, UK, and corresponding author Anna Philpott, Professor of Cancer and Developmental Biology at the University of Cambridge, UK.</p>","PeriodicalId":11375,"journal":{"name":"Development","volume":"151 24","pages":""},"PeriodicalIF":3.7,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142812539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The people behind the papers - Alejandro Berrio and David McClay. 报纸背后的人--亚历杭德罗-贝里奥（Alejandro Berrio）和大卫-麦克雷（David McClay）。
IF 3.7 2区 生物学
Development Pub Date : 2024-12-15 Epub Date: 2024-12-20 DOI: 10.1242/dev.204580
{"title":"The people behind the papers - Alejandro Berrio and David McClay.","authors":"","doi":"10.1242/dev.204580","DOIUrl":"https://doi.org/10.1242/dev.204580","url":null,"abstract":"<p><p>Early sea urchin embryos contain cells called micromeres, which play an important role in the formation of three mesodermal cell types: skeletogenic, blastocoelar and pigment cells. When micromeres are removed, the embryo can replace the skeletogenic and blastocoelar cells via a process called 'transfating', whereby other cells in the embryo step in to take on new roles. However, the pigment cells do not reappear, and the reasons for this are unclear. A new paper in Development reveals how the timing of developmental signals can affect transfating outcomes. To learn more about the story behind the paper, we caught up with first author Alejandro Berrio and corresponding author David McClay, the Arthur S. Pearse Professor Emeritus of Biology at Duke University, USA.</p>","PeriodicalId":11375,"journal":{"name":"Development","volume":"151 24","pages":""},"PeriodicalIF":3.7,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142869417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The unique functions of Runx1 in skeletal muscle maintenance and regeneration are facilitated by an ETS interaction domain. Runx1 在骨骼肌维持和再生方面的独特功能得益于一个 ETS 交互结构域。
IF 3.7 2区 生物学
Development Pub Date : 2024-12-15 Epub Date: 2024-12-12 DOI: 10.1242/dev.202556
Meng Yu, Konrad Thorner, Sreeja Parameswaran, Wei Wei, Chuyue Yu, Xinhua Lin, Raphael Kopan, Matthew R Hass
{"title":"The unique functions of Runx1 in skeletal muscle maintenance and regeneration are facilitated by an ETS interaction domain.","authors":"Meng Yu, Konrad Thorner, Sreeja Parameswaran, Wei Wei, Chuyue Yu, Xinhua Lin, Raphael Kopan, Matthew R Hass","doi":"10.1242/dev.202556","DOIUrl":"10.1242/dev.202556","url":null,"abstract":"<p><p>The conserved Runt-related (RUNX) transcription factor family are master regulators of developmental and regenerative processes. Runx1 and Runx2 are expressed in satellite cells (SCs) and in skeletal myotubes. Here, we examined the role of Runx1 in mouse satellite cells to determine the role of Runx1 during muscle differentiation. Conditional deletion of Runx1 in adult SCs negatively impacted self-renewal and impaired skeletal muscle maintenance even though Runx2 expression persisted. Runx1 deletion in C2C12 cells (which retain Runx2 expression) identified unique molecular functions of Runx1 that could not be compensated for by Runx2. The reduced myoblast fusion in vitro caused by Runx1 loss was due in part to ectopic expression of Mef2c, a target repressed by Runx1. Structure-function analysis demonstrated that the ETS-interacting MID/EID region of Runx1, absent from Runx2, is essential for Runx1 myoblast function and for Etv4 binding. Analysis of ChIP-seq datasets from Runx1 (T cells, muscle)- versus Runx2 (preosteoblasts)-dependent tissues identified a composite ETS:RUNX motif enriched in Runx1-dependent tissues. The ETS:RUNX composite motif was enriched in peaks open exclusively in ATAC-seq datasets from wild-type cells compared to ATAC peaks unique to Runx1 knockout cells. Thus, engagement of a set of targets by the RUNX1/ETS complex define the non-redundant functions of Runx1 in mouse muscle precursor cells.</p>","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11664167/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142603770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Castor is a temporal transcription factor that specifies early born central complex neuron identity.
IF 3.7 2区 生物学
Development Pub Date : 2024-12-15 Epub Date: 2024-12-16 DOI: 10.1242/dev.204318
Noah R Dillon, Chris Q Doe
{"title":"Castor is a temporal transcription factor that specifies early born central complex neuron identity.","authors":"Noah R Dillon, Chris Q Doe","doi":"10.1242/dev.204318","DOIUrl":"10.1242/dev.204318","url":null,"abstract":"<p><p>The generation of neuronal diversity is important for brain function, but how diversity is generated is incompletely understood. We used the development of the Drosophila central complex (CX) to address this question. The CX develops from eight bilateral Type 2 neuroblasts (T2NBs), which generate hundreds of different neuronal types. T2NBs express broad opposing temporal gradients of RNA-binding proteins. It remains unknown whether these protein gradients are sufficient to directly generate all known neuronal diversity, or whether there are temporal transcription factors (TTFs) with narrow expression windows that each specify a small subset of CX neuron identities. Multiple candidate TTFs have been identified, but their function remains uncharacterized. Here, we show that: (1) the adult E-PG neurons are born from early larval T2NBs; (2) the candidate TTF Castor is expressed transiently in early larval T2NBs when E-PG and P-EN neurons are born; and (3) Castor is required to specify early born E-PG and P-EN neuron identities. We conclude that Castor is a TTF in larval T2NB lineages that specifies multiple, early born CX neuron identities.</p>","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142766130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Reprogramming of cells during embryonic transfating: overcoming a reprogramming block.
IF 3.7 2区 生物学
Development Pub Date : 2024-12-15 Epub Date: 2024-12-20 DOI: 10.1242/dev.203152
Alejandro Berrio, Esther Miranda, Abdull J Massri, Anton Afanassiev, Geoffrey Schiebinger, Gregory A Wray, David R McClay
{"title":"Reprogramming of cells during embryonic transfating: overcoming a reprogramming block.","authors":"Alejandro Berrio, Esther Miranda, Abdull J Massri, Anton Afanassiev, Geoffrey Schiebinger, Gregory A Wray, David R McClay","doi":"10.1242/dev.203152","DOIUrl":"10.1242/dev.203152","url":null,"abstract":"<p><p>Regulative development, demonstrated by many animal embryos, is the ability to replace missing cells or parts. The underlying molecular mechanism(s) of that ability is not well understood. If sea urchin micromeres (skeletogenic cell progenitors) are removed at the 16-cell stage, early endoderm initiates a sequential switch in cell fates, called transfating. Without micromeres, other mesoderm cells are absent as well, because their specification depends on signaling from micromeres. Most mesoderm cells later return by transfating, but pigment cells do not. Single-cell RNA sequencing, tracked over time, reveals the reprogramming sequence of those replacements. Beginning with an early endoderm specification state, cells progress through endomesoderm, then mesoderm, and finally distinct skeletogenic and blastocoelar cell specification states emerge, but pigment cells do not. Rescue of pigment cells was found to be a consequence of signal timing: if Delta is expressed prior to Nodal, pigment cells return. Thus, transfating operates through a series of gene regulatory state transitions, and reprogramming fails if endogenous negative signals occur prior to positive signals in the reprogramming sequence.</p>","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142767073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
In preprints: oxygen and NFκB signals shift the timing of hindlimb formation.
IF 3.7 2区 生物学
Development Pub Date : 2024-12-15 Epub Date: 2024-12-19 DOI: 10.1242/dev.204578
Alberto Rosello-Diez, Sergio Menchero
{"title":"In preprints: oxygen and NFκB signals shift the timing of hindlimb formation.","authors":"Alberto Rosello-Diez, Sergio Menchero","doi":"10.1242/dev.204578","DOIUrl":"https://doi.org/10.1242/dev.204578","url":null,"abstract":"","PeriodicalId":11375,"journal":{"name":"Development","volume":"151 24","pages":""},"PeriodicalIF":3.7,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142869416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The people behind the papers - Amanda Pinheiro and Francisco Naya.
IF 3.7 2区 生物学
Development Pub Date : 2024-12-15 Epub Date: 2024-12-20 DOI: 10.1242/dev.204579
{"title":"The people behind the papers - Amanda Pinheiro and Francisco Naya.","authors":"","doi":"10.1242/dev.204579","DOIUrl":"10.1242/dev.204579","url":null,"abstract":"<p><p>The interplay between metabolic pathways and the epigenome is essential for proper cell differentiation. In this new study, Francisco Naya and colleagues find that the Dlk1-Dio3 noncoding RNA (ncRNA) locus regulates cell state by coordinating mitochondrial activity and histone modifications in muscle cells. To find out more about the people behind the work, we caught up with first author Amanda Pinheiro and corresponding author Francisco (Frank) Naya, Associate Professor at the Department of Biology, Boston University, USA.</p>","PeriodicalId":11375,"journal":{"name":"Development","volume":"151 24","pages":""},"PeriodicalIF":3.7,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142869418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Phospho-regulation of ASCL1-mediated chromatin opening during cellular reprogramming. 细胞重编程过程中 ASCL1 介导的染色质开放的磷酸调控。
IF 3.7 2区 生物学
Development Pub Date : 2024-12-15 Epub Date: 2024-12-12 DOI: 10.1242/dev.204329
Roberta Azzarelli, Sarah Gillen, Frances Connor, Jethro Lundie-Brown, Francesca Puletti, Rosalind Drummond, Ana Raffaelli, Anna Philpott
{"title":"Phospho-regulation of ASCL1-mediated chromatin opening during cellular reprogramming.","authors":"Roberta Azzarelli, Sarah Gillen, Frances Connor, Jethro Lundie-Brown, Francesca Puletti, Rosalind Drummond, Ana Raffaelli, Anna Philpott","doi":"10.1242/dev.204329","DOIUrl":"10.1242/dev.204329","url":null,"abstract":"<p><p>The proneural transcription factor ASCL1 regulates neurogenesis and drives somatic cell reprogramming into neurons. However, not all cell types can be reprogrammed by ASCL1, raising the questions of what provides competence and how we can overcome barriers to enable directed differentiation. Here, we investigate how levels of ASCL1 and its phosphorylation modulate its activity over progressive lineage restriction of mouse embryonic stem cells. We find that inhibition of ASCL1 phosphorylation enhances reprogramming of both mesodermal and neuroectodermal cells, while pluripotent cells remain refractory to ASCL1-directed neuronal differentiation. By performing RNA-seq and ATAC-seq in neuroectoderm, we find that un(der)phosphorylated ASCL1 causes increased chromatin accessibility at sites proximal to neuronal genes, accompanied by their increased expression. Combined analysis of protein stability and proneural function of phosphomutant and phosphomimetic ASCL1 reveals that protein stability plays only a marginal role in regulating activity, while changes in amino acid charge cannot fully explain enhanced activity of the serine-proline mutant variants of ASCL1. Our work provides new insights into proneural factor activity and regulation, and suggests ways to optimize reprogramming protocols in cancer and regenerative medicine.</p>","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11664170/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142686161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Conserved roles of engrailed: patterning tissues and specifying cell types.
IF 3.7 2区 生物学
Development Pub Date : 2024-12-15 Epub Date: 2024-12-13 DOI: 10.1242/dev.204250
Alexandra L Joyner, João Ramalho Ortigão-Farias, Thomas Kornberg
{"title":"Conserved roles of engrailed: patterning tissues and specifying cell types.","authors":"Alexandra L Joyner, João Ramalho Ortigão-Farias, Thomas Kornberg","doi":"10.1242/dev.204250","DOIUrl":"10.1242/dev.204250","url":null,"abstract":"<p><p>More than 40 years ago, studies of the Drosophila engrailed and Hox genes led to major discoveries that shaped the history of developmental biology. We learned that these genes define the state of determination of cells that populate particular spatially defined regions: the identity of segmental domains by Hox genes, and the identity of posterior developmental compartments by engrailed. Hence, the boundaries that delimit spatial domains depend on engrailed. Here, we review the engrailed field, which now includes orthologs in Drosophila and mouse, as well as many other animals. We focus on fly and mouse and highlight additional functions that span early stages of embryogenesis and neural development.</p>","PeriodicalId":11375,"journal":{"name":"Development","volume":"151 24","pages":""},"PeriodicalIF":3.7,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11664177/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142817271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A simple MiMIC-based approach for tagging endogenous genes to visualise live transcription in Drosophila. 一种基于 MiMIC 的简单方法，用于标记内源基因以可视化果蝇的实时转录。
IF 3.7 2区 生物学
Development Pub Date : 2024-12-15 Epub Date: 2024-12-16 DOI: 10.1242/dev.204294
Lauren Forbes Beadle, Catherine Sutcliffe, Hilary L Ashe
{"title":"A simple MiMIC-based approach for tagging endogenous genes to visualise live transcription in Drosophila.","authors":"Lauren Forbes Beadle, Catherine Sutcliffe, Hilary L Ashe","doi":"10.1242/dev.204294","DOIUrl":"10.1242/dev.204294","url":null,"abstract":"<p><p>Live imaging of transcription in the Drosophila embryo using the MS2 or PP7 systems is transforming our understanding of transcriptional regulation. However, insertion of MS2/PP7 stem-loops into endogenous genes requires laborious CRISPR genome editing. Here, we exploit the previously described Minos-mediated integration cassette (MiMIC) transposon system in Drosophila to establish a method for simply and rapidly inserting MS2/PP7 cassettes into any of the thousands of genes carrying a MiMIC insertion. In addition to generating a variety of stem-loop donor fly stocks, we have made new stocks expressing the complementary coat proteins fused to different fluorescent proteins. We show the utility of this MiMIC-based approach by MS2/PP7 tagging of endogenous genes and the long non-coding RNA roX1, then imaging their transcription in living embryos. We also present live transcription data from larval brains, the wing disc and ovary, thereby extending the tissues that can be studied using the MS2/PP7 system. Overall, this first high-throughput method for tagging mRNAs in Drosophila will facilitate the study of transcription dynamics of thousands of endogenous genes in a range of Drosophila tissues.</p>","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142709333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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