Development最新文献_第3页

C. elegans epicuticlins define specific compartments in the apical extracellular matrix and function in wound repair. 秀丽隐杆线虫的表皮鞘氨醇确定了细胞外基质顶端的特定区域，并在伤口修复中发挥作用。

IF 3.7 2区生物学

Development Pub Date : 2024-11-01 Epub Date: 2024-10-23 DOI: 10.1242/dev.204330

Murugesan Pooranachithra, Erin M Jyo, Nicolas Brouilly, Nathalie Pujol, Andreas M Ernst, Andrew D Chisholm

{"title":"C. elegans epicuticlins define specific compartments in the apical extracellular matrix and function in wound repair.","authors":"Murugesan Pooranachithra, Erin M Jyo, Nicolas Brouilly, Nathalie Pujol, Andreas M Ernst, Andrew D Chisholm","doi":"10.1242/dev.204330","DOIUrl":"10.1242/dev.204330","url":null,"abstract":"The apical extracellular matrix (aECM) of external epithelia often contains lipid-rich outer layers that contribute to permeability barrier function. The external aECM of nematodes is known as the cuticle and contains an external lipid-rich layer - the epicuticle. Epicuticlins are a family of tandem repeat cuticle proteins of unknown function. Here, we analyze the localization and function of the three C. elegans epicuticlins (EPIC proteins). EPIC-1 and EPIC-2 localize to the surface of the cuticle near the outer lipid layer, as well as to interfacial cuticles and adult-specific struts. EPIC-3 is expressed in dauer larvae and localizes to interfacial aECM in the buccal cavity. Skin wounding in the adult induces epic-3 expression, and EPIC proteins localize to wound sites. Null mutants lacking EPIC proteins are viable with reduced permeability barrier function and normal epicuticle lipid mobility. Loss of function in EPIC genes modifies the skin blistering phenotypes of Bli mutants and reduces survival after skin wounding. Our results suggest EPIC proteins define specific cortical compartments of the aECM and promote wound repair.","PeriodicalId":11375,"journal":{"name":"Development","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11529277/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142380270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Y box-binding protein 1 regulates zebrafish folliculogenesis partly through p21-mediated control of follicle cell proliferation. Y盒结合蛋白1（Ybx1）在斑马鱼卵泡发生中的作用：通过抑制 p21 (cdkn1a) 促进卵泡细胞增殖。

IF 3.7 2区生物学

Development Pub Date : 2024-11-01 Epub Date: 2024-11-08 DOI: 10.1242/dev.202898

Bo Zhu, Zhiwei Zhang, Lakhansing Pardeshi, Yingying Chen, Wei Ge

{"title":"Y box-binding protein 1 regulates zebrafish folliculogenesis partly through p21-mediated control of follicle cell proliferation.","authors":"Bo Zhu, Zhiwei Zhang, Lakhansing Pardeshi, Yingying Chen, Wei Ge","doi":"10.1242/dev.202898","DOIUrl":"10.1242/dev.202898","url":null,"abstract":"Y box-binding protein 1 (Ybx1/ybx1) regulates gene expression through DNA/RNA binding. In zebrafish, Ybx1 is highly abundant in primary growth (PG) follicles in the ovary, but decreases precipitously as the follicles enter the secondary growth (SG). To understand Ybx1 function in folliculogenesis, we created a ybx1 mutant using TALEN and observed disrupted folliculogenesis during the previtellogenic (PV) to early vitellogenic (EV) transition of SG, resulting in underdeveloped ovaries and infertility. Expression and western blot analyses revealed differential gene expression between ybx1-/- and control ovaries, with significantly increased expression of cdkn1a (p21), a cell cycle inhibitor, in ybx1-/- follicles. While cdkn1a knockout via CRISPR/Cas9 was embryonically lethal, the heterozygote (cdkn1a+/-) displayed advanced follicle activation and maturation, contrasting with the ybx1-/- phenotype. Partial loss of p21 alleviated the ybx1-/- phenotype, restoring folliculogenesis with normal PG-PV and PV-EV transitions in ybx1-/-;cdkn1a+/- mutants. While ybx1-/- mutant follicle cells displayed poor proliferation in vivo and in vitro, the cells from the ybx1-/-;cdkn1a+/- follicles resumed normal proliferation. In conclusion, Ybx1 is crucial for early folliculogenesis in zebrafish, potentially by repressing cdkn1a expression, either directly or indirectly.","PeriodicalId":11375,"journal":{"name":"Development","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142521315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Almost 40 years of studying homeobox genes in C. elegans. 研究 elegans 的同源染色体基因近 40 年。

IF 3.7 2区生物学

Development Pub Date : 2024-11-01 Epub Date: 2024-10-30 DOI: 10.1242/dev.204328

Paschalis Kratsios, Oliver Hobert

引用次数: 0

Allometry in limb regeneration and scale-invariant patterning as the basis of normal morphogenesis from different sizes of blastemas. 肢体再生中的异型性和规模不变模式化是不同大小胚胎正常形态发生的基础。

IF 3.7 2区生物学

Development Pub Date : 2024-11-01 Epub Date: 2024-11-08 DOI: 10.1242/dev.202697

Saya Furukawa, Sakiya Yamamoto, Ayaka Ohashi, Yoshihiro Morishita, Akira Satoh

{"title":"Allometry in limb regeneration and scale-invariant patterning as the basis of normal morphogenesis from different sizes of blastemas.","authors":"Saya Furukawa, Sakiya Yamamoto, Ayaka Ohashi, Yoshihiro Morishita, Akira Satoh","doi":"10.1242/dev.202697","DOIUrl":"10.1242/dev.202697","url":null,"abstract":"Axolotl (Ambystoma mexicanum) limb regeneration begins with blastemas of various sizes, in contrast to the limb developmental process. Despite this size variation, normal limb morphology, consistent with a limb stump size, is regenerated. This outcome suggests the existence of underlying scale-invariant mechanisms. To identify such mechanisms, we examined the allometric relationships between blastema size, and Sonic Hedgehog (Shh) and Fibroblast Growth Factor 8 (Fgf8) expression patterns against limb stump size. We found that all factors showed allometric rather than isometric scaling; specifically, their relative sizes decrease with an increase in limb stump size. However, the ratio of Shh/Fgf8 signaling dominant region was nearly constant, independent of blastema/body size. Furthermore, the relative spatial patterns of cell density and proliferation activity, and the relative position of first digit formation were scale invariant in the summed Shh/Fgf8 crosstalk region. This scale-invariant nature may underlie the morphogenesis of normal limbs from different sizes of blastemas.","PeriodicalId":11375,"journal":{"name":"Development","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142343713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Target gene responses differ when transcription factor levels are acutely decreased by nuclear export versus degradation. 当转录因子水平因核输出和降解而急剧下降时，目标基因的反应也不同。

IF 3.7 2区生物学

Development Pub Date : 2024-11-01 Epub Date: 2024-11-08 DOI: 10.1242/dev.202775

James McGehee, Angelike Stathopoulos

{"title":"Target gene responses differ when transcription factor levels are acutely decreased by nuclear export versus degradation.","authors":"James McGehee, Angelike Stathopoulos","doi":"10.1242/dev.202775","DOIUrl":"10.1242/dev.202775","url":null,"abstract":"Defining the time of action for morphogens requires tools capable of temporally controlled perturbations. To study how the transcription factor Dorsal affects patterning of the Drosophila embryonic dorsal-ventral axis, we used two light-inducible tags that trigger either nuclear export or degradation of Dorsal under blue light. Nuclear export of Dorsal leads to loss of the high-threshold, ventrally expressed target gene snail (sna), while the low-threshold, laterally expressed target gene short-gastrulation (sog) is retained. In contrast, degradation of Dorsal results in retention of sna, loss of sog, and lower nuclear levels compared to when Dorsal is exported from the nucleus. To understand why nuclear export causes loss of sna but degradation does not, we investigated Dorsal kinetics using photobleaching and found that it rapidly re-enters the nucleus even under blue-light conditions favoring export. The associated kinetics of Dorsal being rapidly imported and exported continuously are likely responsible for loss of sna but, alternatively, can support sog. Collectively, our results indicate that this dynamic patterning process is influenced by both Dorsal concentration and nuclear retention.","PeriodicalId":11375,"journal":{"name":"Development","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142460560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The people behind the papers - Saya Furukawa, Akira Satoh and Yoshihiro Morishita. 报纸背后的人--古川沙耶、佐藤明和森下义弘。

IF 3.7 2区生物学

Development Pub Date : 2024-11-01 Epub Date: 2024-11-08 DOI: 10.1242/dev.204443

引用次数: 0

Tracking and measuring local protein synthesis in vivo. 跟踪和测量体内局部蛋白质合成

IF 3.7 2区生物学

Development Pub Date : 2024-11-01 DOI: 10.1242/dev.202908

Ibrahim Kays, Brian E Chen

引用次数: 0

Jag1 represses Notch activation in lateral supporting cells and inhibits an outer hair cell fate in the medial cochlea. Jag1 可抑制外侧支持细胞的 Notch 激活，并抑制内侧耳蜗外毛细胞的命运。

IF 3.7 2区生物学

Development Pub Date : 2024-11-01 Epub Date: 2024-11-05 DOI: 10.1242/dev.202949

Sandra de Haan, Agustin A Corbat, Christopher R Cederroth, Lisa G Autrum, Simona Hankeova, Elizabeth C Driver, Barbara Canlon, Matthew W Kelley, Emma R Andersson

{"title":"Jag1 represses Notch activation in lateral supporting cells and inhibits an outer hair cell fate in the medial cochlea.","authors":"Sandra de Haan, Agustin A Corbat, Christopher R Cederroth, Lisa G Autrum, Simona Hankeova, Elizabeth C Driver, Barbara Canlon, Matthew W Kelley, Emma R Andersson","doi":"10.1242/dev.202949","DOIUrl":"10.1242/dev.202949","url":null,"abstract":"Notch signaling patterns the cochlear organ of Corti, and individuals with the JAG1/NOTCH2-related genetic disorder Alagille syndrome can thus experience hearing loss. We investigated the function of Jag1 in cochlear patterning and signaling using Jag1Ndr/Ndr mice, which are a model of Alagille syndrome. Jag1Ndr/Ndr mice exhibited expected vestibular and auditory deficits, a dose-dependent increase in ectopic inner hair cells, and a reduction in outer hair cells. Single cell RNA sequencing of the organ of Corti demonstrated a global dysregulation of genes associated with inner ear development and deafness. Analysis of individual cell types further revealed that Jag1 represses Notch activation in lateral supporting cells and demonstrated a function for Jag1 in gene regulation and development of outer hair cells. Surprisingly, ectopic 'outer hair cell-like' cells were present in the medial compartment and pillar cell region of Jag1Ndr/Ndr cochleae, yet they exhibited location-dependent expression of the inner hair cell fate-determinant Tbx2, suggesting Jag1 is required for Tbx2 to drive inner hair cell commitment. This study thus identifies new roles for Jag1 in supporting cells, and in outer hair cell specification and positioning.","PeriodicalId":11375,"journal":{"name":"Development","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142380272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Testosterone acts through membrane protein GPRC6A to cause cardiac edema in zebrafish embryos. 睾酮通过膜蛋白 GPRC6A 作用于斑马鱼胚胎，导致心脏水肿。

IF 3.7 2区生物学

Development Pub Date : 2024-10-31 DOI: 10.1242/dev.204390

Vahid Zadmajid, Shayan Shahriar, Daniel A Gorelick

{"title":"Testosterone acts through membrane protein GPRC6A to cause cardiac edema in zebrafish embryos.","authors":"Vahid Zadmajid, Shayan Shahriar, Daniel A Gorelick","doi":"10.1242/dev.204390","DOIUrl":"10.1242/dev.204390","url":null,"abstract":"Androgens are classically thought to act through intracellular androgen receptors (AR/NR3C4), but they can also trigger non-genomic effects via membrane proteins. Although several membrane androgen receptors have been characterized in vitro, their functions in vivo remain unclear. Using a chemical-genetic screen in zebrafish, we found that GPRC6A, a G-protein coupled receptor, mediates non-genomic androgen actions during embryonic development. Exposure to androgens (androstanedione, DHT, and testosterone) caused cardiac edema or tail curvature in wild-type embryos, as well as in ar mutants, suggesting AR-independent pathways. We then mutated putative membrane androgen receptors (gprc6a, hcar1-4, and zip9) and found that only gprc6a mutants exhibited a significant reduction in cardiac edema following testosterone exposure. Additionally, co-treatment of wild-type embryos with testosterone and GPRC6A antagonists significantly suppressed the cardiac edema phenotype. Using RNA-seq and RNA rescue approaches, we found that testosterone-GPRC6A causes cardiac phenotypes by reducing Pak1 signaling. Our results indicate that testosterone induces cardiac edema in zebrafish embryos through GPRC6A, independent of nuclear androgen receptors, highlighting a novel non-genomic androgen signaling pathway in embryonic development.","PeriodicalId":11375,"journal":{"name":"Development","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142544370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Germ cell progression through zebrafish spermatogenesis declines with age. 斑马鱼精子发生过程中生殖细胞的发育随年龄增长而衰退。

IF 3.7 2区生物学

Development Pub Date : 2024-10-29 DOI: 10.1242/dev.204319

Andrea L Sposato, Hailey L Hollins, Darren R Llewellyn, Jenna M Weber, Madison N Schrock, Jeffrey A Farrell, James A Gagnon

引用次数: 0