Development最新文献_第3页

The dynamics of tubulogenesis in development and disease.

IF 3.7 2区生物学

Development Pub Date : 2025-02-01 Epub Date: 2025-02-17 DOI: 10.1242/dev.202820

Adrian Romero, Brandy L Walker, Vanja Krneta-Stankic, Kamryn Gerner-Mauro, Lydia Youmans, Rachel K Miller

引用次数: 0

Correction: Transcriptional dynamics during karyogamy in rice zygotes.

IF 3.7 2区生物学

Development Pub Date : 2025-02-01 Epub Date: 2025-02-11 DOI: 10.1242/dev.204691

Erika Toda, Shizuka Koshimizu, Atsuko Kinoshita, Tetsuya Higashiyama, Takeshi Izawa, Kentaro Yano, Takashi Okamoto

引用次数: 0

Interplay of SHH, WNT and BMP4 signaling regulates the development of the lamina propria in the murine ureter. SHH、WNT和BMP4信号的相互作用调节小鼠输尿管固有层的发育。

IF 3.7 2区生物学

Development Pub Date : 2025-02-01 Epub Date: 2025-02-06 DOI: 10.1242/dev.204214

Philipp Straube, Anja Beckers, Ulrich W H Jany, Florian Bergmann, Timo H-W Lüdtke, Carsten Rudat, Mark-Oliver Trowe, Imke Peters, Maximilian G Klopf, Tamrat M Mamo, Andreas Kispert

{"title":"Interplay of SHH, WNT and BMP4 signaling regulates the development of the lamina propria in the murine ureter.","authors":"Philipp Straube, Anja Beckers, Ulrich W H Jany, Florian Bergmann, Timo H-W Lüdtke, Carsten Rudat, Mark-Oliver Trowe, Imke Peters, Maximilian G Klopf, Tamrat M Mamo, Andreas Kispert","doi":"10.1242/dev.204214","DOIUrl":"10.1242/dev.204214","url":null,"abstract":"In mammalian ureters, the lamina propria presents as a prominent layer of connective tissue underneath the urothelium. Despite its important structural and signaling functions, little is known how the lamina propria develops. Here, we show that in the murine ureter the lamina propria arises at late fetal stages and massively increases by fibrocyte proliferation and collagen deposition after birth. WNT, SHH, BMP4 and retinoic acid signaling are all active in the common mesenchymal progenitor of smooth muscle cells and lamina propria fibrocytes. However, around birth, the lamina propria becomes a target for epithelial WNT and SHH signals and a source of BMP4 and retinoic acid. SHH and WNT signaling promote lamina propria and smooth muscle cell differentiation and proliferation at fetal and early postnatal stages, whereas BMP4 signaling is required for early smooth muscle cell differentiation but not for its later maintenance. Our findings suggest that, in the presence of SHH and WNT signaling, it is the modulation of BMP4 signaling which is the major determinant for the segregation of lamina propria and smooth muscle cells.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11829765/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143001977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development: a journal's journey.

IF 3.7 2区生物学

Development Pub Date : 2025-02-01 Epub Date: 2025-02-10 DOI: 10.1242/dev.204602

Alex Eve

引用次数: 0

Cdkn1c orchestrates a molecular network that regulates the euploidy of the male mouse germline stem cells.

IF 3.7 2区生物学

Development Pub Date : 2025-01-24 DOI: 10.1242/dev.204286

Mito Kanatsu-Shinohara, Takuya Yamamoto, Tianjiao Liu, Keiichi I Nakayama, Takashi Shinohara

{"title":"Cdkn1c orchestrates a molecular network that regulates the euploidy of the male mouse germline stem cells.","authors":"Mito Kanatsu-Shinohara, Takuya Yamamoto, Tianjiao Liu, Keiichi I Nakayama, Takashi Shinohara","doi":"10.1242/dev.204286","DOIUrl":"https://doi.org/10.1242/dev.204286","url":null,"abstract":"Karyotype instability in the germline leads to infertility. Unlike the female germline, the male germline continuously produces fertile sperm throughout life. Here we present a molecular network responsible for maintaining karyotype stability in the male mouse germline. Loss of the cyclin-dependent kinase inhibitor Cdkn1c in undifferentiated spermatogonia induced degeneration of spermatogenesis prior to entry into the differentiating spermatogonia. In vitro analysis of spermatogonial stem cells (SSCs) revealed that CDKN1C localized to spindle microtubules during metaphase, and that disupted microtubule dynamics increased its phosphorylation. Cdkn1c deficiency activated the spindle assembly checkpoint and led to centrosome amplification, premature chromosome segregation, and loss of AURKB, and ultimately TRP53-dependent apoptosis. Trp53-deficient SSCs exhibited karyotype defects, but proliferated normally despite reduced CDKN1C and AURKB expression. In contrast, Aurkb depletion upregulated TRP53 and CDKN1C, suggesting a negative feedback loop to maintain euploidy. Thus, Cdkn1c regulates the male germline karyotype.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143028321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sox10 is required for systemic initiation of bone mineralization. Sox10是骨矿化系统启动所必需的。

IF 3.7 2区生物学

Development Pub Date : 2025-01-15 Epub Date: 2025-01-20 DOI: 10.1242/dev.204357

Stefani Gjorcheska, Sandhya Paudel, Sarah McLeod, David Paulding, Louisa Snape, Karen Camargo Sosa, Cunming Duan, Robert Kelsh, Lindsey Barske

{"title":"Sox10 is required for systemic initiation of bone mineralization.","authors":"Stefani Gjorcheska, Sandhya Paudel, Sarah McLeod, David Paulding, Louisa Snape, Karen Camargo Sosa, Cunming Duan, Robert Kelsh, Lindsey Barske","doi":"10.1242/dev.204357","DOIUrl":"10.1242/dev.204357","url":null,"abstract":"Heterozygous variants in SOX10 cause congenital syndromes affecting pigmentation, digestion, hearing, and neural development, primarily attributable to failed differentiation or loss of non-skeletal neural crest derivatives. We report here an additional, previously undescribed requirement for Sox10 in bone mineralization. Neither crest- nor mesoderm-derived bones initiate mineralization on time in zebrafish sox10 mutants, despite normal osteoblast differentiation and matrix production. Mutants are deficient in the Trpv6+ ionocytes that take up calcium from the environment, resulting in severe calcium deficiency. As these ionocytes derive from ectoderm, not crest, we hypothesized that the primary defect resides in a separate organ that systemically regulates ionocyte numbers. RNA sequencing revealed significantly elevated stanniocalcin (Stc1a), an anti-hypercalcemic hormone, in sox10 mutants. Stc1a inhibits calcium uptake in fish by repressing trpv6 expression and Trpv6+ ionocyte proliferation. Epistasis assays confirm excess Stc1a as the proximate cause of the calcium deficit. The pronephros-derived glands that synthesize Stc1a interact with sox10+ cells, but these cells are missing in mutants. We conclude that sox10+ crest-derived cells non-autonomously limit Stc1a production to allow the inaugural wave of calcium uptake necessary to initiate bone mineralization.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11833171/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142946435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulation of cell cycle in plant gametes: when is the right time to divide? 植物配子细胞周期的调控：何时是分裂的最佳时机？

IF 3.7 2区生物学

Development Pub Date : 2025-01-15 Epub Date: 2025-01-20 DOI: 10.1242/dev.204217

Sara Simonini

引用次数: 0

The WIRS motifs in Fat2 are required for Drosophila egg chamber rotation but not for elongation. Fat2中的WIRS基序是果蝇卵室旋转所必需的，但不是延长所必需的。

IF 3.7 2区生物学

Development Pub Date : 2025-01-15 Epub Date: 2025-01-17 DOI: 10.1242/dev.204201

Akanksha Bhatt, Valentin Ruffine, Uwe Töpfer, Jinhee Ryu, Elisabeth Fischer-Friedrich, Christian Dahmann

{"title":"The WIRS motifs in Fat2 are required for Drosophila egg chamber rotation but not for elongation.","authors":"Akanksha Bhatt, Valentin Ruffine, Uwe Töpfer, Jinhee Ryu, Elisabeth Fischer-Friedrich, Christian Dahmann","doi":"10.1242/dev.204201","DOIUrl":"10.1242/dev.204201","url":null,"abstract":"The elongation of tissues and organs is important for proper morphogenesis in animal development. In Drosophila ovaries, the elongation of egg chambers involves aligned Collagen IV fiber-like structures, a gradient of extracellular matrix stiffness and actin-based protrusion-driven collective cell migration, leading to the rotation of the egg chamber. Egg chamber elongation and rotation depend on the atypical cadherin Fat2. Fat2 contains in its intracellular region three WRC interacting receptor sequence (WIRS) motifs, which previously had been shown to bind to the WAVE regulatory complex (WRC), a conserved actin regulator. Here, we show that in fat2 mutant flies lacking the WIRS motifs, egg chambers fail to rotate and Collagen IV fiber-like structures are impaired, yet a gradient of extracellular matrix stiffness is established and egg chambers properly elongate. We conclude that the WIRS motifs are required for egg chamber rotation and that egg chamber rotation might be a prerequisite for proper formation of Collagen IV fiber-like structures. Egg chamber rotation, however, is dispensable for extracellular matrix stiffness gradient formation and for egg chamber elongation.","PeriodicalId":11375,"journal":{"name":"Development","volume":"152 2","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11829772/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143001989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In preprints: giving the developing brain the energy it needs. 在预印本中：给予发育中的大脑所需的能量。

IF 3.7 2区生物学

Development Pub Date : 2025-01-15 Epub Date: 2025-01-16 DOI: 10.1242/dev.204594

Taylor R Pennington, Madeline G Andrews

引用次数: 0

Transcriptional dynamics during karyogamy in rice zygotes. 水稻受精卵核分裂过程中的转录动力学。

IF 3.7 2区生物学

Development Pub Date : 2025-01-15 Epub Date: 2025-01-27 DOI: 10.1242/dev.204497

Erika Toda, Shizuka Koshimizu, Atsuko Kinoshita, Tetsuya Higashiyama, Takeshi Izawa, Kentaro Yano, Takashi Okamoto

{"title":"Transcriptional dynamics during karyogamy in rice zygotes.","authors":"Erika Toda, Shizuka Koshimizu, Atsuko Kinoshita, Tetsuya Higashiyama, Takeshi Izawa, Kentaro Yano, Takashi Okamoto","doi":"10.1242/dev.204497","DOIUrl":"10.1242/dev.204497","url":null,"abstract":"Upon fertilization, male and female nuclei fuse to form the zygotic nucleus in angiosperms. Karyogamy is considered to be essential for proper embryogenesis; however, the transcriptional dynamics during karyogamy in plant zygotes remain unclear. In this study, we performed a single-cell transcriptome analysis of rice zygotes at six early developmental stages (15 min, 30 min, 1 h, 2 h, 4 h, and 6 h after gamete fusion) to reveal gene expression profiles during karyogamy in plant zygotes. The time-series RNA-sequencing analysis detected possible de novo and altered gene expression in zygotes from 15 min post-fertilization. Fertilization-induced transcription during karyogamy was characterized by protein interaction database and gene ontology (GO) analyses. Furthermore, paternal allele transcription was initiated approximately 30 min to 1 h after gamete fusion, when nuclear fusion begins in the zygote. Some transcripts preferentially expressed in egg cells were downregulated after gamete fusion. Moreover, a dynamic shift from maternal-biased transcripts to bi-parental expression occurred during early zygotic development. These results suggest that transcriptional dynamics during karyogamy plays an initial role in proper and sequential zygotic development and embryogenesis.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11829756/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142946436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0