Development最新文献_第3页

FG repeats drive co-clustering of nuclear pores and P granules in the C. elegans germline. 在线虫种系中，FG重复序列驱动核孔和P颗粒的共聚。

IF 3.7 2区生物学

Development Pub Date : 2025-03-15 Epub Date: 2025-03-27 DOI: 10.1242/dev.204585

Laura L Thomas, Devavrat M Bodas, Geraldine Seydoux

引用次数: 0

Long-term labelling and tracing of endodermal cells using a perpetual cycling Gal4-UAS system. 使用永久循环Gal4-UAS系统对内胚层细胞进行长期标记和追踪。

IF 3.7 2区生物学

Development Pub Date : 2025-03-15 Epub Date: 2025-03-21 DOI: 10.1242/dev.204289

Yanfeng Li, You Li, Bangzhuo Huang, Ruhao Zhang, Jianbo He, Lingfei Luo, Yun Yang

{"title":"Long-term labelling and tracing of endodermal cells using a perpetual cycling Gal4-UAS system.","authors":"Yanfeng Li, You Li, Bangzhuo Huang, Ruhao Zhang, Jianbo He, Lingfei Luo, Yun Yang","doi":"10.1242/dev.204289","DOIUrl":"10.1242/dev.204289","url":null,"abstract":"Cell labelling and lineage tracing are indispensable tools in developmental biology, offering powerful means with which to visualise and understand the complex dynamics of cell populations during embryogenesis. Traditional cell labelling relies heavily on signal stability, promoter strength and stage specificity, limiting its application in long-term tracing. In this report, we optimise and reconfigure a perpetual cycling Gal4-UAS system employing a previously unreported Gal4 fusion protein and the autoregulatory Gal4 expression loop. As validated through heat-shock induction, this configuration ensures sustained transcription of reporter genes in target cells and their descendant cells while minimising cytotoxicity, thereby achieving long-term labelling and tracing. Further exploiting this system, we generate zebrafish transgenic lines with continuous fluorescent labelling specific to the endoderm, and demonstrate its effectiveness in long-term tracing by showing the progression of endoderm development from embryo to adult, providing visualisation of endodermal cells and their derived tissues. This continuous labelling and tracing strategy can span the entire process of endodermal differentiation, from progenitor cells to mature functional cells, and is applicable to studying endoderm patterning and organogenesis.","PeriodicalId":11375,"journal":{"name":"Development","volume":"152 6","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11959616/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143669395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The people behind the papers - Laura Lahti and Thomas Perlmann. 这些报纸背后的人——劳拉·拉赫蒂和托马斯·帕尔曼。

IF 3.7 2区生物学

Development Pub Date : 2025-03-15 Epub Date: 2025-03-26 DOI: 10.1242/dev.204787

引用次数: 0

Correction: Vascular endothelial growth factor c regulates hematopoietic stem cell fate in the dorsal aorta. 更正：血管内皮生长因子c调节背主动脉内造血干细胞的命运。

IF 3.7 2区生物学

Development Pub Date : 2025-03-15 Epub Date: 2025-03-27 DOI: 10.1242/dev.204754

Rebecca K Schiavo, Owen J Tamplin

引用次数: 0

Sox9 and nuclear factor I transcription factors regulate the timing of neurogenesis and ependymal maturation in dopamine progenitors. Sox9和Nfi转录因子调节多巴胺祖细胞神经发生和室管膜成熟的时间。

IF 3.7 2区生物学

Development Pub Date : 2025-03-15 Epub Date: 2025-03-26 DOI: 10.1242/dev.204421

Laura Lahti, Nikolaos Volakakis, Linda Gillberg, Behzad Yaghmaeian Salmani, Katarína Tiklová, Nigel Kee, Hilda Lundén-Miguel, Maarten Werkman, Michael Piper, Richard Gronostajski, Thomas Perlmann

{"title":"Sox9 and nuclear factor I transcription factors regulate the timing of neurogenesis and ependymal maturation in dopamine progenitors.","authors":"Laura Lahti, Nikolaos Volakakis, Linda Gillberg, Behzad Yaghmaeian Salmani, Katarína Tiklová, Nigel Kee, Hilda Lundén-Miguel, Maarten Werkman, Michael Piper, Richard Gronostajski, Thomas Perlmann","doi":"10.1242/dev.204421","DOIUrl":"10.1242/dev.204421","url":null,"abstract":"Correct timing of neurogenesis is crucial for generating the correct number and subtypes of glia and neurons in the embryo, and for preventing tumours and stem cell depletion in the adults. Here, we analyse how the midbrain dopamine (mDA) neuron progenitors transition into cell cycle arrest (G0) and begin to mature into ependymal cells. Comparison of mDA progenitors from different embryonic stages revealed upregulation of the genes encoding Sox9 and nuclear factor I transcription factors during development. Their conditional inactivation in the early embryonic midbrain led to delayed G0 entry and ependymal maturation in the entire midbrain ventricular zone, reduced gliogenesis and increased generation of neurons, including mDA neurons. In contrast, their inactivation in late embryogenesis did not result in mitotic re-entry, suggesting that these factors are necessary for G0 induction, but not for its maintenance. Our characterisation of adult ependymal cells by single-cell RNA sequencing and histology show that mDA-progenitor-derived cells retain several progenitor features but also secrete neuropeptides and contact neighbouring cells and blood vessels, indicating that these cells may form part of the circumventricular organ system.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143490469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optogenetic manipulation of nuclear Dorsal reveals temporal requirements and consequences for transcription. 核背侧的光遗传学操作揭示了转录的时间要求和后果。

IF 3.7 2区生物学

Development Pub Date : 2025-03-15 Epub Date: 2025-03-31 DOI: 10.1242/dev.204706

Virginia L Pimmett, James McGehee, Antonio Trullo, Maria Douaihy, Ovidiu Radulescu, Angelike Stathopoulos, Mounia Lagha

{"title":"Optogenetic manipulation of nuclear Dorsal reveals temporal requirements and consequences for transcription.","authors":"Virginia L Pimmett, James McGehee, Antonio Trullo, Maria Douaihy, Ovidiu Radulescu, Angelike Stathopoulos, Mounia Lagha","doi":"10.1242/dev.204706","DOIUrl":"10.1242/dev.204706","url":null,"abstract":"Morphogen gradients convey essential spatial information during tissue patterning. Although the concentration and timing of morphogen exposure are both crucial, how cells interpret these graded inputs remains challenging to address. We employed an optogenetic system to acutely and reversibly modulate the nuclear concentration of the morphogen Dorsal (DL), homolog of NF-κB, which orchestrates dorsoventral patterning in the Drosophila embryo. By controlling DL nuclear concentration while simultaneously recording target gene outputs in real time, we identified a critical window for DL action that is required to instruct patterning and characterized the resulting effect on spatiotemporal transcription of target genes in terms of timing, coordination and bursting. We found that a transient decrease in nuclear DL levels at nuclear cycle 13 leads to reduced expression of the mesoderm-associated gene snail (sna) and partial derepression of the neurogenic ectoderm-associated target short gastrulation (sog) in ventral regions. Surprisingly, the mispatterning elicited by this transient change in DL was detectable at the level of single-cell transcriptional bursting kinetics, specifically affecting long inter-burst durations. Our approach of using temporally resolved and reversible modulation of a morphogen in vivo, combined with mathematical modeling, establishes a framework for understanding the stimulus-response relationships that govern embryonic patterning.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143522788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dynamic interactions between cartilaginous and tendinous/ligamentous primordia during musculoskeletal integration. 肌肉骨骼整合过程中软骨和肌腱/韧带原基之间的动态相互作用。

IF 3.7 2区生物学

Development Pub Date : 2025-03-15 Epub Date: 2025-03-26 DOI: 10.1242/dev.204512

Xinyi Yu, Ryosuke Kawakami, Shinsei Yambe, Yuki Yoshimoto, Takako Sasaki, Shinnosuke Higuchi, Hitomi Watanabe, Haruhiko Akiyama, Shigenori Miura, Kadi Hu, Gen Kondoh, Ramu Sagasaki, Masafumi Inui, Taiji Adachi, Denitsa Docheva, Takeshi Imamura, Chisa Shukunami

{"title":"Dynamic interactions between cartilaginous and tendinous/ligamentous primordia during musculoskeletal integration.","authors":"Xinyi Yu, Ryosuke Kawakami, Shinsei Yambe, Yuki Yoshimoto, Takako Sasaki, Shinnosuke Higuchi, Hitomi Watanabe, Haruhiko Akiyama, Shigenori Miura, Kadi Hu, Gen Kondoh, Ramu Sagasaki, Masafumi Inui, Taiji Adachi, Denitsa Docheva, Takeshi Imamura, Chisa Shukunami","doi":"10.1242/dev.204512","DOIUrl":"https://doi.org/10.1242/dev.204512","url":null,"abstract":"Proper connections between cartilaginous and muscular primordia through tendinous/ligamentous primordia are essential for musculoskeletal integration. Herein, we report a novel double-reporter mouse model for investigating this process via fluorescently visualising scleraxis (Scx) and SRY-box containing gene 9 (Sox9) expression. We generated ScxTomato transgenic mice and crossed them with Sox9EGFP knock-in mice to obtain ScxTomato;Sox9EGFP mice. Deep imaging of optically cleared double-reporter embryos at E13.5 and E16.5 revealed previously unknown differences in the dynamic interactions between cartilaginous and tendinous/ligamentous primordia in control and Scx-deficient mice. Tendon/ligament maturation was evaluated through simultaneous detection of fluorescence and visualisation of collagen fibre formation using second harmonic generation imaging. Lack of deltoid tuberosity in Scx-deficient mice caused misdirected muscle attachment with morphological changes. Loss of Scx also dysregulated progenitor cell fate determination in the chondrotendinous junction, resulting in the formation of a rounded enthesis rather than the protruding enthesis observed in the control. Hence, our double-reporter mouse system, in combination with loss- or gain-of-function approaches, is a unique and powerful tool that could be used to gain a comprehensive understanding of musculoskeletal integration.","PeriodicalId":11375,"journal":{"name":"Development","volume":"152 6","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143709378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In preprints: the ins and outs of Cnidaria germ layers. 预印本：刺胞菌胚层的来龙去脉。

IF 3.7 2区生物学

Development Pub Date : 2025-03-15 Epub Date: 2025-03-19 DOI: 10.1242/dev.204730

Lucas Leclère, Chiara Sinigaglia

引用次数: 0

Science under siege: protecting scientific progress in turbulent times. 围攻中的科学：在动荡时期保护科学进步。

IF 3.7 2区生物学

Development Pub Date : 2025-03-15 Epub Date: 2025-03-13 DOI: 10.1242/dev.204757

James Briscoe, Craig E Franklin, Daniel A Gorelick, E Elizabeth Patton, Michael Way

引用次数: 0

The KMT2 complex protein ASH2L is required for meiotic prophase progression but dispensable for mitosis in differentiated spermatogonia. KMT2复合蛋白ASH2L是减数分裂前期进展所必需的，但在分化的精原细胞中有丝分裂是必不可少的。

IF 3.7 2区生物学

Development Pub Date : 2025-03-15 Epub Date: 2025-03-27 DOI: 10.1242/dev.204630

Zhen Lin, Bowen Rong, Meixia Wu, Junyi Yan, Tong Hong, Linjun Hou, Xinzhe Tang, Qiang Liu, Xiaozhong Peng, Yao Chen, Fei Lan, Ming-Han Tong

{"title":"The KMT2 complex protein ASH2L is required for meiotic prophase progression but dispensable for mitosis in differentiated spermatogonia.","authors":"Zhen Lin, Bowen Rong, Meixia Wu, Junyi Yan, Tong Hong, Linjun Hou, Xinzhe Tang, Qiang Liu, Xiaozhong Peng, Yao Chen, Fei Lan, Ming-Han Tong","doi":"10.1242/dev.204630","DOIUrl":"10.1242/dev.204630","url":null,"abstract":"ASH2L is a core component of KMT2 complexes, crucial for H3K4 trimethylation. However, its role in spermatogenesis remains elusive. Here, we demonstrate an essential role of Ash2l for meiotic prophase but dispensable for mitosis in differentiated spermatogonia. Using a germ cell-specific Ash2l knockout mouse model, we reveal that Ash2l deficiency leads to meiotic arrest and sterility in both sexes. Ash2l-deficient spermatocytes exhibit failures in chromosomal synapsis associated with persistent DMC1 foci and γH2AX, resulting in meiocyte loss due to apoptosis. Conversely, Ash2l-deficient differentiated spermatogonia show normal development. Mechanistically, Ash2l deficiency results in a global loss of H3K4me3 in promoter regions and significantly decreases expression of thousands of genes. Among these are genes involved in epigenetic silencing pathways, such as H3K9 di-methylation, DNA methylation and piRNA pathways, that are crucial for transposon repression during meiotic prophase I progression. Supporting this, we observe that Ash2l mutant spermatocytes display ectopic expression of LINE1-ORF1P. Our findings therefore reveal the previously unappreciated role of ASH2L-dependent H3K4me3 modification in spermatogenesis and provide clues to the molecular mechanisms in epigenetic disorders underlying male infertility.","PeriodicalId":11375,"journal":{"name":"Development","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143482527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0