GATA factor Serpent promotes phagocytosis in non-professional phagocytes during Drosophila oogenesis.

IF 3.7 2区生物学 Q1 DEVELOPMENTAL BIOLOGY

Development Pub Date : 2025-03-26 DOI:10.1242/dev.204464

Baosheng Zeng, Haley Grayson, Jianjun Sun

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Abstract

Clearance of dying cells is essential for tissue homeostasis and requires both professional and non-professional phagocytes; however, it is unclear what promotes phagocytosis by non-professional phagocytes. Follicle cells of Drosophila egg chambers function as non-professional phagocytes to clear large germ cell debris in mid and late oogenesis, providing an excellent model for the study of non-professional phagocytes. Here we demonstrate that GATA factor Serpent (Srp) plays an indispensable role in promoting the phagocytic capacity of follicle cells in both processes. Srp is upregulated in follicle cells of degenerating mid-stage egg chambers, and its knockdown results in incomplete clearance of germ cell debris and premature follicle cell death. In addition, Srp is upregulated in stretch follicle cells and essential for clearing the nurse cell nuclei in late oogenesis. Genetic analysis reveals that Srp acts downstream of JNK signaling to upregulate the expression of the phagocytic receptor Draper (Drpr) as well as other components in the corpse processing machinery. Our findings highlight the crucial role for Srp in non-professional phagocytes during Drosophila oogenesis, which may also be conserved across species.

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GATA因子Serpent在果蝇卵发生过程中促进非专业吞噬细胞的吞噬。

死亡细胞的清除对组织稳态至关重要，需要专业和非专业的吞噬细胞；然而，目前尚不清楚是什么促进了非专业吞噬细胞的吞噬作用。果蝇卵室卵泡细胞作为非专业吞噬细胞，在卵发生中后期清除较大的生殖细胞碎片，为非专业吞噬细胞的研究提供了良好的模型。在这两个过程中，GATA因子Serpent （Srp）在促进卵泡细胞的吞噬能力中起着不可或缺的作用。Srp在变性中期卵室的卵泡细胞中上调，其下调导致生殖细胞碎片的不完全清除和卵泡细胞过早死亡。此外，Srp在拉伸卵泡细胞中表达上调，对于卵子发生后期清除护理细胞核至关重要。遗传分析表明，Srp作用于JNK信号的下游，上调吞噬受体Drpr（吞噬受体Drpr）以及尸体处理机制中其他成分的表达。我们的研究结果强调了Srp在果蝇卵发生过程中对非专业吞噬细胞的关键作用，这也可能在物种间保守。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Development 生物-发育生物学

CiteScore

6.70

自引率

4.30%

发文量

433

审稿时长

3 months

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