Current Microbiology最新文献_第7页

Heterochromatin Protein Activates the Amylase Expression Pathway and Its Application to Recombinant Protein Expression in Penicillium oxalicum. 异染色质蛋白激活草青霉淀粉酶表达途径及其在重组蛋白表达中的应用。

IF 2.3 3区生物学

Current Microbiology Pub Date : 2025-01-09 DOI: 10.1007/s00284-024-04058-0

Demin Guo, Shengfang Zhao, Jie Chen, Shuhui Han, Yangtao Li, Yu Chen, Shengbiao Hu, Yibo Hu

{"title":"Heterochromatin Protein Activates the Amylase Expression Pathway and Its Application to Recombinant Protein Expression in Penicillium oxalicum.","authors":"Demin Guo, Shengfang Zhao, Jie Chen, Shuhui Han, Yangtao Li, Yu Chen, Shengbiao Hu, Yibo Hu","doi":"10.1007/s00284-024-04058-0","DOIUrl":"10.1007/s00284-024-04058-0","url":null,"abstract":"Remodelling regulatory pathways to directionally increase the efficiency of specific promoters in chassis cells is an effective strategy for the rational construction of expression systems. However, the repeated utilization of one regulator to modify the host cell to improve expression motif efficiency has a limited effect. Therefore, it is preferable to identify new regulatory factors to activate specific pathways and thus further improve the efficiency of target elements. Heterochromatin protein 1 (HP1) is considered a main factor responsible for heterochromatin maintenance; it binds DNA and thus forms a tight structure to repress gene expression in fungi. This study revealed that the overexpression of HepA (a homologue of HP1) increased amylase expression in Penicillium oxalicum. Furthermore, HepA was overexpressed in two engineered strains in which the endoglucanase TaEG and amylase Amy15B were recombinantly expressed under the control of the amylase promoter Pamy15A, resulting in increased production of these two enzymes. Therefore, HepA could be used as a novel facilitator to modify Penicillium chassis cells, in which the efficiency of expression motifs located in the amylase pathway can be further strengthened.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 2","pages":"75"},"PeriodicalIF":2.3,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142946383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In silico MLVA Analysis of Brucella melitensis from Human and Livestock in Iran. 伊朗人畜源布鲁氏菌的硅基MLVA分析。

IF 2.3 3区生物学

Current Microbiology Pub Date : 2025-01-08 DOI: 10.1007/s00284-024-03940-1

Maryam Dadar, Saeed Alamian

{"title":"In silico MLVA Analysis of Brucella melitensis from Human and Livestock in Iran.","authors":"Maryam Dadar, Saeed Alamian","doi":"10.1007/s00284-024-03940-1","DOIUrl":"10.1007/s00284-024-03940-1","url":null,"abstract":"Brucellosis, a zoonotic disease caused by Brucella spp. globally, is of great significance not only to livestock but also to public health. The most significant of the twelve species is Brucella melitensis. This article is devoted to the endemic region of Iran and aims to uncover the molecular epidemiology of B. melitensis. Biotyping, AMOS-PCR, Bruce-ladder PCR, and the in silico method of MLVA were employed to test 40 B. melitensis isolates from humans, cows, sheep, goats, camels, and horses which are found in thirteen Iranian provinces throughout the years 2015 to 2022. The data from the MLVA-8 analysis showed that there were seven genotypes that could be identified, and the most commonly identified genotype was genotype 63. The data from the MLVA-10 analysis showed that there were seven genotypes, with genotype 213 being the most prevalent in Iran. The data from the MLVA-11 analysis showed that there were eight genotypes, with genotype 111 being the most prevalent in Iran. The MLVA and SNP analysis results showed that the bacteria were grouped into two main groups, known as the Eastern Mediterranean and American groups. Moreover, the outcomes from these analyses have added considerably to our understanding of the genetic/historical relationships among the isolates. Our study indicates a high prevalence of B. melitensis biovar 1 in Iran, accounting for 82.5% of the isolates. The study provides insight into such matters as the complex epidemiology of B. melitensis in Iran, suggesting different ways of transmission and sources of infection. This research points out the vital significance of the continuation of the surveillance and curation of B. melitensis in the diverse species of animals and humans. The simplicity and efficiency of MLVA-based molecular epidemiology offer information on the geographic distribution and genetic diversity of B. melitensis and, therefore, help in the devising of targeted strategies for the prevention of disease in animals.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 2","pages":"74"},"PeriodicalIF":2.3,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142946393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization and Correlation Analysis of Bacterial Composition and Physicochemical Quality in High- and Medium-Temperature Daqu from China's Binzhou Region.

IF 2.3 3区生物学

Current Microbiology Pub Date : 2025-01-06 DOI: 10.1007/s00284-024-04037-5

Mengjun Cui, Wenchao Cai, Peirong Yu, Wei Chen, Chunhui Shan, Guo Zhuang, Yurong Wang

{"title":"Characterization and Correlation Analysis of Bacterial Composition and Physicochemical Quality in High- and Medium-Temperature Daqu from China's Binzhou Region.","authors":"Mengjun Cui, Wenchao Cai, Peirong Yu, Wei Chen, Chunhui Shan, Guo Zhuang, Yurong Wang","doi":"10.1007/s00284-024-04037-5","DOIUrl":"https://doi.org/10.1007/s00284-024-04037-5","url":null,"abstract":"To investigate the bacterial community structure and physicochemical characteristics of different types of Daqu in the Binzhou region, this study employed traditional pure culture methods, high-throughput sequencing technology, and conventional physicochemical assays for analysis. The research results indicate that Enterococcus faecium and Bacillus licheniformis emerged as the main LAB and Bacillus species in Daqu from Binzhou region, respectively. In addition, high-throughput sequencing revealed significant differences in bacterial community structure between the two types of Daqu (P < 0.01). Compostibacillus and Sebaldella were identified as the biomarkers and potential key strains of high- and medium-temperature Daqu, respectively, and high-temperature Daqu demonstrated higher microbial complexity and stability than medium-temperature Daqu. Physicochemical assays demonstrated that the a* value, Daqu skin hardness, Daqu core hardness, density, starch content, and aminophenol content being significantly higher in high-temperature Daqu (P < 0.05), meanwhile, the L* value, water activity, water content, protein content, liquefaction power, and saccharification power were found to be significantly lower in high-temperature Daqu (P < 0.05). And there was significant association between dominant genera and the physicochemical indexes of Daqu (P = 0.001). It can thus be seen that there were significant differences between the microbial communities and physicochemical indicators of different types of Daqu in the Binzhou region. The results of this study are of great significance for further analyzing the differences between different types of Daqu and improving their quality.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 2","pages":"72"},"PeriodicalIF":2.3,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143064177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanisms of Staphylococcus aureus Antibiotics Resistance Revealed by Adaptive Laboratory Evolution. 金黄色葡萄球菌耐药性的实验室适应性进化机制

IF 2.3 3区生物学

Current Microbiology Pub Date : 2025-01-06 DOI: 10.1007/s00284-024-03980-7

Shiwei Ma, Yufan Xu, Juanjuan Ma, Dan Luo, Zixin Huang, Longlong Wang, Weile Xie, Zhen Luo, Huanhuan Zhang, Jijie Jiang, Yaozhong Jin, Jianming Zhang, Jianguo Zhu, Zhe Wang

{"title":"Mechanisms of Staphylococcus aureus Antibiotics Resistance Revealed by Adaptive Laboratory Evolution.","authors":"Shiwei Ma, Yufan Xu, Juanjuan Ma, Dan Luo, Zixin Huang, Longlong Wang, Weile Xie, Zhen Luo, Huanhuan Zhang, Jijie Jiang, Yaozhong Jin, Jianming Zhang, Jianguo Zhu, Zhe Wang","doi":"10.1007/s00284-024-03980-7","DOIUrl":"https://doi.org/10.1007/s00284-024-03980-7","url":null,"abstract":"Infection caused by drug-resistant Staphylococcus aureus is a serious public health and veterinary concern. Lack of a comprehensive understanding of the mechanisms underlying the emergence of drug-resistant strains, it makes S. aureus one of the most intractable pathogenic bacteria. To identify mutations that confer resistance to anti-S. aureus drugs, we established a laboratory-based adaptive evolution system and performed 10 rounds of evolution experiments against 15 clinically used antibiotics. We discovered a panel of known and novel resistance-associated sites after performing whole-genome sequencing. Furthermore, we found that the resistance evolved at distinct rates. For example, streptomycin, rifampicin, fusidic acid and novobiocin all developed significant resistance quickly in the second round of evolution. Intriguingly, the cross-resistance experiment reveals that nearly all drug-resistant strains have varying degrees of increased sensitivity to fusidic acid, pointing to a novel approach to battle AMR. In addition, the in silico docking analysis shows that the evolved mutants affect the interaction of rifampcin-rpoB, as well as the novobiocin-gyrB. Moreover, for the genes we got in the laboratory evolution, mutant genes of clinical isolates of human had significant differences from the environmental isolates and animal isolates. We believe that the strategy and data set in this research will be helpful for battling AMR issue of S. aureus, and adaptable to other pathogenic microbes.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 1","pages":"46"},"PeriodicalIF":2.3,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142962139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diversity of Soil Microbial Communities in the Bulk and Rhizosphere Soils of Acanthopanax senticosus in Different Habitats.

IF 2.3 3区生物学

Current Microbiology Pub Date : 2025-01-06 DOI: 10.1007/s00284-024-04054-4

Wenhui Yuan, Deqiang Yang, Zhipeng Xu, Yunwei Liu, Xiangquan Li, Changhong Ding, Zhibin Wang

{"title":"Diversity of Soil Microbial Communities in the Bulk and Rhizosphere Soils of Acanthopanax senticosus in Different Habitats.","authors":"Wenhui Yuan, Deqiang Yang, Zhipeng Xu, Yunwei Liu, Xiangquan Li, Changhong Ding, Zhibin Wang","doi":"10.1007/s00284-024-04054-4","DOIUrl":"https://doi.org/10.1007/s00284-024-04054-4","url":null,"abstract":"Comprehending the interplay between the microbial communities of bulk soil (BS) and rhizosphere soil (RS) holds crucial significance in maintaining soil health and fertility, as well as enhancing crop quality. Our research focused on examining these microbial communities in BS and RS of Acanthopanax senticosus, along with their correlation with soil nutrients, across three distinct habitats in Yichun, Heilongjiang Province. To achieve this, we employed high-throughput sequencing technology, specifically targeting the 16S and amplicon regions. The results showed that there were significant differences in soil nutrients, microbial diversity and composition between BS and RS in different habitats. Available phosphorus (AP), total nitrogen (TN), ammonium-nitrogen (NH4+-N) and available potassium (AK) content in RS were higher than that in BS, but TP content was opposite. The Mantel experiment's findings revealed that the soil physicochemical attributes exerted a more significant impact on the microbial community present in RS compared to its influence in BS. Redundancy analysis (RDA) indicated that the bacterial community of BS was affected by SMC and TP. The fungal structure of BS was affected by soil moisture content (SMC), AP, AK (P < 0.01) and TN (P < 0.05). The fungal structure of RS was affected by soil organic carbon (SOC), AP and AK. A noteworthy inverse relationship was observed between Actinomycetes and SMC as well as AP. In conclusion, these results extend the understanding of soil microbial community of A. senticosus and provide a preliminary understanding of the effects of habitat changes caused by human activities on A. senticosus.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 2","pages":"73"},"PeriodicalIF":2.3,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143064118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anaeroselena agilis gen. nov., sp. nov., a Novel Sulfite- and Arsenate-Respiring Bacterium Within the Family Acetonemataceae Isolated from a Thermal Spring of North Ossetia. 从北奥塞梯温泉中分离的一种新的亚硫酸盐和砷呼吸细菌——厌氧selena agilis gen. nov., sp. nov.

IF 2.3 3区生物学

Current Microbiology Pub Date : 2025-01-05 DOI: 10.1007/s00284-024-04046-4

Maria I Prokofeva, Alexander G Elcheninov, Alexandra A Klyukina, Andrei A Novikov, Gennady S Kachmazov, Stepan V Toshchakov, Evgenii N Frolov, Olga A Podosokorskaya

{"title":"Anaeroselena agilis gen. nov., sp. nov., a Novel Sulfite- and Arsenate-Respiring Bacterium Within the Family Acetonemataceae Isolated from a Thermal Spring of North Ossetia.","authors":"Maria I Prokofeva, Alexander G Elcheninov, Alexandra A Klyukina, Andrei A Novikov, Gennady S Kachmazov, Stepan V Toshchakov, Evgenii N Frolov, Olga A Podosokorskaya","doi":"10.1007/s00284-024-04046-4","DOIUrl":"10.1007/s00284-024-04046-4","url":null,"abstract":"A novel Gram-negative, motile, rod-shaped bacterium, designated 4137-clT, was isolated from a thermal spring of North Ossetia (Russian Federation). Strain 4137-clT grew at 30-50 °C (optimum 42 °C) with 0-3.5% NaCl (optimum 0-0.3%) and within pH range 4.0-8.7 (optimum pH 6.8-7.3). It was a strictly anaerobic microorganism capable of fermentation and respiration on organic acids and proteinaceous substrates. Sulfur, sulfite, polysulfide, and arsenate were used as electron acceptors. In addition to heterotrophic growth it grew autotrophically with H2/CO2. The major fatty acids were C16:1 ω8c and C16:0. The size of the genome and genomic DNA G+C content of strain 4137-clT were 4.5 Mb and 59.2%, respectively. According to the 16S rRNA gene sequence and conserved protein sequences phylogenies, strain 4137-clT represented a distinct lineage of the family Acetonemataceae within the class Negativicutes. As inferred from the morphology, physiology, chemotaxonomical and phylogenomic analyses, strain 4137-clT ought to be recognized as a novel genus for which the name Anaeroselena agilis gen. nov., sp. nov., we propose. The type strain is 4137-clT(=KCTC 25383T = VKM B-3575T).","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 2","pages":"71"},"PeriodicalIF":2.3,"publicationDate":"2025-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142930972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Translation Initiation Factor eIF2Bα Regulates Development, Stress Response, Amylase Production, and Kojic Acid Synthesis in the Fungus Aspergillus oryzae. 翻译起始因子eIF2Bα调控米曲霉的发育、胁迫反应、淀粉酶生成和曲酸合成。

IF 2.3 3区生物学

Current Microbiology Pub Date : 2025-01-05 DOI: 10.1007/s00284-024-04051-7

Yiling Liu, Zixin Chen, Chaofeng Chang, Yifen Lin, Guiyi Zheng, Feng Zhang

{"title":"The Translation Initiation Factor eIF2Bα Regulates Development, Stress Response, Amylase Production, and Kojic Acid Synthesis in the Fungus Aspergillus oryzae.","authors":"Yiling Liu, Zixin Chen, Chaofeng Chang, Yifen Lin, Guiyi Zheng, Feng Zhang","doi":"10.1007/s00284-024-04051-7","DOIUrl":"10.1007/s00284-024-04051-7","url":null,"abstract":"Translation initiation, which involves numerous protein factors and coordinated control steps, represents the most complicated process during eukaryotic translation. However, the roles of eukaryotic translation initiation factor (eIF) in filamentous fungi are not well clarified. In this study, we investigated the function of eIF2Bα in Aspergillus oryzae, an industrially important filamentous fungus. The ΔeIF2Bα mutants showed slow colony growth and decreased conidia production, suggesting the critical roles of eIF2Bα in the growth and development of A. oryzae. In addition, the loss of eIF2Bα significantly impaired the ability to produce amylase and kojic acid, indicating the involvement of eIF2Bα in the amylase synthesis and secondary metabolite production. Interestingly, the elimination of eIF2Bα improved the tolerance of A. oryzae to diverse adverse stresses, including endoplasmic reticulum stress, oxidative stress, cell wall-perturbing stress, and cell membrane-damaging stress. Overall, our results indicate that eIF2Bα is a crucial regulator of growth, development, stress response, amylase production, and kojic acid synthesis in A. oryzae.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 2","pages":"70"},"PeriodicalIF":2.3,"publicationDate":"2025-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142930974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Limnobacter olei sp. nov., a Novel Diesel-Degrading Bacterium Isolated from Oil-Contaminated Soil. 油污染土壤中分离的一种新型柴油降解细菌——油酸Limnobacter olei sp. nov.。

IF 2.3 3区生物学

Current Microbiology Pub Date : 2025-01-04 DOI: 10.1007/s00284-024-04053-5

Yongchuang Liu, Siqiong Xu, Jialiang Li, Yujie Ouyang, Shuai Gao, Pan Yang, Cuiwei Chu, Jian He, Tongwen Yang, Keshi Ma, Lili Li, Chao Wang

{"title":"Limnobacter olei sp. nov., a Novel Diesel-Degrading Bacterium Isolated from Oil-Contaminated Soil.","authors":"Yongchuang Liu, Siqiong Xu, Jialiang Li, Yujie Ouyang, Shuai Gao, Pan Yang, Cuiwei Chu, Jian He, Tongwen Yang, Keshi Ma, Lili Li, Chao Wang","doi":"10.1007/s00284-024-04053-5","DOIUrl":"10.1007/s00284-024-04053-5","url":null,"abstract":"A bacterial strain P1T, capable of degrading diesel and converting thiosulfate to sulfate was isolated from an oil-contaminated soil sample. The cells were Gram-stain-negative, slightly curved rods and motile with a single polar flagellum. Growth of the strain was observed at 4-45 °C (optimum at 28 °C), at pH 4.0-12.0 (optimum at pH 10.0) and with 0-15.0% (w/v) NaCl (optimum at 2.0%). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain P1T was closely related to the members of the genus Limnobacter, with the highest sequence similarity to Limnobacter thiooxidans DSM 13612 T (99.8%), followed by Limnobacter alexandrii LZ-4 T (99.4%), Limnobacter parvus YS8-69 T (98.8%), Limnobacter litoralis KP1-19 T (97.6%), and Limnobacter humi UCM-39 T (97.5%). The draft genome sequence of strain P1T was 3.40 Mb long, with a DNA G + C content of 52.4%. The average nucleotide identity and digital DNA-DNA hybridization values between strain P1T and the closely related type strains were in the range of 71.8-85.1% and 18.1-28.7%, respectively. The predominant cellular fatty acids of strain P1T included C16: 0, summed feature 3 (C16: 1 ɷ7c and/or C16: 1 ɷ6c), summed feature 8 (C18: 1 ɷ7c and/or C18: 1 ɷ6c), and summed feature 7 (C19: 1 ɷ6c and/or C19: 1 ɷ7c and/or C19: 1 cyclo). In addition, the main polar lipid was composed of diphosphatidylglycerol, phosphatidylethanolamine, and phosphatidylglycerol. Q-8 was the sole respiratory quinone. Based on the polyphasic characterization, strain P1T (= KCTC 72814 T = CCTCC AB 2019403 T) represents a novel species of the genus Limnobacter, for which the name Limnobacter olei sp. nov. is proposed.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 2","pages":"69"},"PeriodicalIF":2.3,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142926789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Endophytic Microbial Community Structure and Dynamics Influence Agarwood Formation in Aquilaria malaccensis Lam. 沉香内生微生物群落结构与动态对沉香形成的影响

IF 2.3 3区生物学

Current Microbiology Pub Date : 2025-01-03 DOI: 10.1007/s00284-024-04048-2

Sudipta Sankar Bora, Ruponsing Ronghang, Pompi Das, Romen Singh Naorem, Dibya Jyoti Hazarika, Rahul Gogoi, Sofia Banu, Madhumita Barooah

{"title":"Endophytic Microbial Community Structure and Dynamics Influence Agarwood Formation in Aquilaria malaccensis Lam.","authors":"Sudipta Sankar Bora, Ruponsing Ronghang, Pompi Das, Romen Singh Naorem, Dibya Jyoti Hazarika, Rahul Gogoi, Sofia Banu, Madhumita Barooah","doi":"10.1007/s00284-024-04048-2","DOIUrl":"10.1007/s00284-024-04048-2","url":null,"abstract":"Aquilaria malaccensis Lam., an Agarwood-producing tree native to Southeast Asia, secretes oleoresin, a resin with diverse applications, in response to injuries. To explore the role of endosphere microbial communities during Agarwood development, we utilized a metagenomics approach across three stages: non-symptomatic (NC), symptomatic early (IN), and symptomatic mature (IN1). The NC metagenome was dominated by Bacillus (19.15%), Klebsiella (13.25%), and Pantoea (12.46%) among bacteria and Saccharomyces (15.92%) among fungi. Notably, bacterial chemotaxis pathway genes were more prevalent in NC (2.14%) compared to IN (0.92%) and IN1 (1.16%), suggesting microbial chemotactic behavior. In the IN stage, Klebsiella (27.05%) and Saccharomyces (34.81%) were the dominant genera. The IN1 metagenome featured Pantoea (8.92%) and Neurospora (8.24%) as leading bacterial and fungal genera, respectively. Functional genes associated with defense mechanisms, lipid transport, and secondary metabolite biosynthesis were increasingly represented in IN1, indicating an enhanced microbial response as infection progresses. Ecological indices, including a high Shannon-Wiener index (H' = 4.467) and Simpson's dominance (1 - D = 0.9697), alongside Pielou's evenness index (J = 0.7034), highlighted a dynamic and diverse microbial community at the mature infection stage, reflecting the complex interactions within the Aquilaria endosphere during Agarwood formation.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 2","pages":"66"},"PeriodicalIF":2.3,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142926785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification, Biocontrol and Plant Growth Promotion Potential of Endophytic Streptomyces sp. a13. 内生链霉菌（Streptomyces sp. a13）的鉴定、生物防治及促生潜力

IF 2.3 3区生物学

Current Microbiology Pub Date : 2025-01-03 DOI: 10.1007/s00284-024-04009-9

Chingakham Juliya Devi, Kangkon Saikia, Rajkumari Mazumdar, Rictika Das, Pranami Bharadwaj, Debajit Thakur

{"title":"Identification, Biocontrol and Plant Growth Promotion Potential of Endophytic Streptomyces sp. a13.","authors":"Chingakham Juliya Devi, Kangkon Saikia, Rajkumari Mazumdar, Rictika Das, Pranami Bharadwaj, Debajit Thakur","doi":"10.1007/s00284-024-04009-9","DOIUrl":"10.1007/s00284-024-04009-9","url":null,"abstract":"Medicinal plants often harbour various endophytic actinomycetia, which are well known for their potent antimicrobial properties and plant growth-promoting traits. In this study, we isolated an endophytic actinomycetia, A13, from the leaves of tea clone P312 from the MEG Tea Estate, Meghalaya, India. The isolate A13 was identified as Streptomyces sp. A13 through whole genome sequencing (WGS) and 16S rRNA sequencing, showing 88% (ANI; Average Nucleotide Identity) and 99.78% sequence similarity with Streptomyces olivaceus. The strain A13 exhibited a prominent broad-spectrum antifungal activity against nine phytopathogens. It was observed that the ethyl acetate (EtAc) extract of A13 inhibits the spore germination rate of phytopathogen Nigrospora sphaerica (NSP) and also damages the fungal cell wall and cell structure. Additionally, the A13 strain exhibits several plant growth-promoting (PGP) traits, such as nitrogen fixation, ammonia production (4.7 µmol/ml), indole-acetic acid (IAA) production (8.91 µg/ml), siderophore production and 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity Gas chromatography mass spectrometry (GC-MS) analysis revealed that Phenol, 3,5-bis(1,1-dimethylethyl) was found to be the major chemical constituent in the EtAc extract of the A13 strain, accounting for 50.15% of the area percentage. Whole genome sequencing and subsequent genome analysis utilizing bioinformatics techniques such as Antibiotics & Secondary Metabolite Analysis SHell (antiSMASH) and Rapid Annotation using Subsystem Technology (RAST) revealed a wide array of biologically active secondary metabolite biosynthesis gene clusters (smBGCs) with different physiologically significant roles. These findings emphasize the potential of the A13 strain as a biocontrol agent with the capability to enhance plant growth and prevent diseases.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 2","pages":"64"},"PeriodicalIF":2.3,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142920875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0