Current Microbiology最新文献

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Host and Viral Factors Influencing Chronic Hepatitis B Infection Across Three Generations in a Family. 影响一个家庭三代人慢性乙型肝炎感染的宿主和病毒因素。
IF 2.3 3区 生物学
Current Microbiology Pub Date : 2024-11-05 DOI: 10.1007/s00284-024-03963-8
Malihe Naderi, Seyed Masoud Hosseini, Naser Behnampour, Sima Besharat, Iraj Shahramian, Masoud Khoshnia, Abdolvahab Moradi
{"title":"Host and Viral Factors Influencing Chronic Hepatitis B Infection Across Three Generations in a Family.","authors":"Malihe Naderi, Seyed Masoud Hosseini, Naser Behnampour, Sima Besharat, Iraj Shahramian, Masoud Khoshnia, Abdolvahab Moradi","doi":"10.1007/s00284-024-03963-8","DOIUrl":"10.1007/s00284-024-03963-8","url":null,"abstract":"<p><p>Chronic hepatitis B (CHB) infection is influenced by both virological and host factors. A total of 5,920 CHB patients were classified into four groups based on HBV seromarkers: three-generation families (CHB grandmother, mother, and child), two-generation families (CHB mother/child pairs), individuals recovered from HBV infection, and a control group. Serological markers, viral load, liver function tests (LFT), HBV mutations, HLA-DQ variations, cytokine polymorphisms, and liver stiffness measurements (LSM) were analyzed using FibroScan. Point mutations in genes such as core/pre-core (G1896A/G1899A), polymerase (H248N, H267Q, N263D), S (G145R, S143L), and X (C1500T, T1464C) were observed in 30% of three-generation pairs and 20% of two-generation pairs. The three-generation group exhibited the highest mean liver stiffness measurement (LSM) (4.94 ± 1.24 kPa), which is considered a predictor for the development of hepatocellular carcinoma (HCC). Subsequent HLA allele analysis identified HLA-DQB105:01 (OR = 0.27) as a risk factor for treatment resistance, while HLA-DQB105 (OR = 0.98), HLA-DQB103 (OR = 0.80), and HLA-DQB104:01 (OR = 0.70) were associated with HBV persistence in both three- and two-generation groups. Higher frequencies of specific polymorphisms, including G/G (TNF-α: 75%; IL-18: 74%), A/A (IL-10: 74.28%), and C/C (IL-1ß: 80%), were significantly linked to persistent infection. Analysis of viral sequences, HLA-DQB1 variations, cytokine polymorphisms, and genetic relationships within the phylogenetic tree revealed that 40% of CHB patients from three-generation families were infected by a shared source of transmission, as indicated by the presence of the same HBV genotype. This study underscores the complex interplay of host and viral factors that influence hepatitis B infection outcomes and suggests potential familial transmission pathways.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"81 12","pages":"446"},"PeriodicalIF":2.3,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142582254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Acute Phytotoxicity and Antifungal Effect of Nanochitosan Particles on Colletotrichum fructicola with Low Susceptibility to Chitosan. 纳米壳聚糖颗粒对壳聚糖敏感性低的果孢子菌的急性植物毒性和抗真菌效果
IF 2.3 3区 生物学
Current Microbiology Pub Date : 2024-11-05 DOI: 10.1007/s00284-024-03909-0
Laura Stephany López-Bermúdez, Eber Addí Quintana-Obregón, Ema Carina Rosas-Burgos, Alma Carolina Gálvez-Iriqui, Porfirio Gutiérrez-Martínez, Jaime Lizardi-Mendoza, Maribel Plascencia-Jatomea
{"title":"Acute Phytotoxicity and Antifungal Effect of Nanochitosan Particles on Colletotrichum fructicola with Low Susceptibility to Chitosan.","authors":"Laura Stephany López-Bermúdez, Eber Addí Quintana-Obregón, Ema Carina Rosas-Burgos, Alma Carolina Gálvez-Iriqui, Porfirio Gutiérrez-Martínez, Jaime Lizardi-Mendoza, Maribel Plascencia-Jatomea","doi":"10.1007/s00284-024-03909-0","DOIUrl":"10.1007/s00284-024-03909-0","url":null,"abstract":"<p><p>Colletotrichum fungus complex affects several crops and tropical products, which suffer significant losses due to anthracnose. The use of chitosan nanoparticles (CNPs), alone or in combination with bioactive compounds, has been recommend for agronomic applications. However, there is very little information on their phytotoxicity, and there is no information about the effect on microorganisms with low susceptibility to chitosan. This work aims to compare their antifungal effect against isolates of C. fructicola with low susceptibility to chitosan and to study the toxicological effects of CNPs on the germination of lettuce (Lactuca sativa) and radish (Raphanus sativus) seeds. Levels of phytotoxicity of high-to-very high and moderate-to-very high were observed for lettuce and radish seeds, respectively, with greater detrimental effects on the radicle elongation after exposure to CNPs concentrations of 4-5 g L<sup>-1</sup>. For the three C. fructicola isolates, the CNPs did not inhibit the fungal growth; however, the cell viability decreased as the CNPs concentration increased, and a complete inhibition of the viability was found for H4-1 and 003 isolates at a CNPs concentration of 5 g L<sup>-1</sup>. Morphometric alterations characterized by a reduction in the average length of the terminal hyphae, distortion, and a higher number of branches in the hyphae, were observed. To our knowledge, this is the first report where the effect of nanochitosan particles in Colletotrichum fructicola, with experimentally proven low sensitivity to chitosan, was studied.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"81 12","pages":"445"},"PeriodicalIF":2.3,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142582245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genome-Based Classification of 'Haloarcula aidinensis' and Description of Three Novel Halophilic Archaea Isolated from an Inland Saline Lake, Coastal Saline Soil, and a Marine Solar Saltern. 基于基因组的 "Haloarcula aidinensis "分类以及从内陆盐湖、沿海盐渍土和海洋日光盐层中分离出的三种新型嗜卤古细菌的描述。
IF 2.3 3区 生物学
Current Microbiology Pub Date : 2024-11-04 DOI: 10.1007/s00284-024-03970-9
Xue Ma, Yao Hu, Jing Hou, Yu-Guang Zhou, Heng-Lin Cui
{"title":"Genome-Based Classification of 'Haloarcula aidinensis' and Description of Three Novel Halophilic Archaea Isolated from an Inland Saline Lake, Coastal Saline Soil, and a Marine Solar Saltern.","authors":"Xue Ma, Yao Hu, Jing Hou, Yu-Guang Zhou, Heng-Lin Cui","doi":"10.1007/s00284-024-03970-9","DOIUrl":"10.1007/s00284-024-03970-9","url":null,"abstract":"<p><p>'Haloarcula aidinensis' was described by a pioneer Chinese scientist focused on halophilic archaea thirty years ago, and the type strain of 'Haloarcula aidinensis' was recently classified based on phylogenetic, phylogenomic, and comparative genomic analyses. Other three novel halophilic archaeal strains, CK38<sup>T</sup>, DT43<sup>T</sup>, and SYNS111<sup>T</sup>, isolated from diverse saline environments in China, were simultaneously subjected to polyphasic classification. 'Haloarcula aidinensis' A<sub>5</sub> was found to be related to Haloarcula amylolytica, while strains CK38<sup>T</sup>, DT43<sup>T</sup>, and SYNS111<sup>T</sup> represented three novel species of Haloarcula based on phylogenetic, phylogenomic, and comparative genomic analyses. These strains can be distinguished from other species within the genus Haloarcula based on multiple phenotypic characteristics. The major phospholipids, phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, and phosphatidylglycerol sulfate can be detected in these strains, while the glycolipid profiles of these strains are diverse. Strains A<sub>5</sub> and DT43<sup>T</sup> contained glucosyl mannosyl glucosyl diether and a diglycosyl diether, while other strains CK38<sup>T</sup> and SYNS111<sup>T</sup> had sulfated mannosyl glucosyl diether and mannosyl glucosyl diether. Thus, strain A<sub>5</sub> should be a reference strain of Har. amylolytica and three novel species of Haloarcula, Haloarcula sediminis sp. nov., Haloarcula brevis sp. nov., and Haloarcula regularis sp. nov. are proposed to accommodate strains CK38<sup>T</sup> (= CGMCC 1.62732<sup>T</sup> = JCM 36675<sup>T</sup>), DT43<sup>T</sup> (= CGMCC 1.18924<sup>T</sup> = JCM 36146<sup>T</sup>), and SYNS111<sup>T</sup> (= CGMCC 1.62601<sup>T</sup> = JCM 36149<sup>T</sup>).</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"81 12","pages":"442"},"PeriodicalIF":2.3,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142567911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Antifungal Associations with a Polyelectrolyte Promote Significant Reduction of Minimum Inhibitory Concentrations against Opportunistic Candida spp. Strains. 抗真菌剂与聚电解质的结合可显著降低对机会性念珠菌菌株的最低抑制浓度。
IF 2.3 3区 生物学
Current Microbiology Pub Date : 2024-11-04 DOI: 10.1007/s00284-024-03960-x
Lavínia da V Pereira, Tiago Rizzi, Micaela Federizzi, Katarzyna Z Donato, Ricardo K Donato, Alexandre M Fuentefria, Paula Reginatto
{"title":"Antifungal Associations with a Polyelectrolyte Promote Significant Reduction of Minimum Inhibitory Concentrations against Opportunistic Candida spp. Strains.","authors":"Lavínia da V Pereira, Tiago Rizzi, Micaela Federizzi, Katarzyna Z Donato, Ricardo K Donato, Alexandre M Fuentefria, Paula Reginatto","doi":"10.1007/s00284-024-03960-x","DOIUrl":"10.1007/s00284-024-03960-x","url":null,"abstract":"<p><p>The current global scenario presents us with a growing increase in infections caused by fungi, referred to by specialists in the field as a \"silent epidemic\", aggravated by the limited pharmacological arsenal and increasing resistance to this therapy. For this reason, drug repositioning and therapeutic compound combinations are promising strategies to mitigate this serious problem. In this context, this study investigates the antifungal activity of the non-toxic, low-cost and widely available cationic polyelectrolyte Poly(diallyldimethylammonium chloride) (PDDA), in combination with different antifungal drugs: systemic (amphotericin B, AMB), topical (clioquinol, CLIO) and oral (nitroxoline, NTX). For each combination, different drug:PDDA ratios were tested and, through the broth microdilution technique, the minimum inhibitory concentration (MIC) of these drugs in the different ratios against clinically important Candida species strains was determined. Overall, PDDA combinations with the studied drugs demonstrated a significant increase in drug activity against most strains, reaching MIC reductions of up to 512 fold for the fluconazole resistant Candida krusei (Pichia kudriavzevii). In particular, the AMB-PDDA combination 1:99 was highly effective against AMB-resistant strains, demonstrating the excellent profile of PDDA as an adjuvant/association in novel antifungal formulations with outdated conventional drugs.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"81 12","pages":"441"},"PeriodicalIF":2.3,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142567759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Performance Evaluation of Native Plant Growth-Promoting Bacteria Associated with Organic Tea Plantations for Development of Bioinoculants for Crop Plants. 对有机茶园中促进植物生长的本地细菌进行性能评估,以开发用于农作物的生物接种剂。
IF 2.3 3区 生物学
Current Microbiology Pub Date : 2024-11-04 DOI: 10.1007/s00284-024-03962-9
Stevenson Thabah, S R Joshi
{"title":"Performance Evaluation of Native Plant Growth-Promoting Bacteria Associated with Organic Tea Plantations for Development of Bioinoculants for Crop Plants.","authors":"Stevenson Thabah, S R Joshi","doi":"10.1007/s00284-024-03962-9","DOIUrl":"10.1007/s00284-024-03962-9","url":null,"abstract":"<p><p>This study aimed at isolation of native plant growth-promoting bacteria (PGPB) associated with organic tea plantations. Most research on tea and associated microbes have been on Darjeeling and Assam, known for their world-class tea. However, emerging tea plantations in remote Northeast India are gaining prominence due to their unique geographical location, favorable climate, and organic practices. This study investigated PGBP associated with these organic tea plantations, aimed to assess their potential cross-infectivity on non-host plants. A total of 58 PGP bacterial isolates were isolated from four organic tea plantations. Six potential isolates were further evaluated individually and as consortium for their PGP on rice and maize. Bacillus, Pseudomonas, and Serratia spp. as individual and in consortium were found to have potent cross-infectivity with significant growth promotion in non-host plants indicated by plant height, root length, shoot, and root weight. The present findings suggest that PGPB native to organic tea plantations have potential cross-infectivity for use as a biofertilizers to improve the growth and productivity of non-host crops. This provides prospectives of using native bacteria on non-host plants paving the way for their potential application in sustainable agriculture practices for growth promotion of staple food crops.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"81 12","pages":"444"},"PeriodicalIF":2.3,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142567891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Existence of Thermotolerant and Salt-Loving Diarrheagenic Vibrio alginolyticus in Non-Saline Potable Water System: A Novel Finding from India. 非碱性饮用水系统中存在耐热和喜盐的致腹泻藻溶性弧菌:印度的一项新发现
IF 2.3 3区 生物学
Current Microbiology Pub Date : 2024-11-04 DOI: 10.1007/s00284-024-03939-8
Suvajit Saha, Subham Mookerjee, Anup Palit
{"title":"Existence of Thermotolerant and Salt-Loving Diarrheagenic Vibrio alginolyticus in Non-Saline Potable Water System: A Novel Finding from India.","authors":"Suvajit Saha, Subham Mookerjee, Anup Palit","doi":"10.1007/s00284-024-03939-8","DOIUrl":"10.1007/s00284-024-03939-8","url":null,"abstract":"<p><p>We present a novel report of abundance of halophilic Vibrio alginolyticus with thermotolerant and enterotoxigenic characteristics from community water system of an inland-focus of India causing diarrheal outbreak as an index pathogen. Though, Vibrio alginolyticus causing diarrhea after exposure to marine water and consumption of seafood was reported globally, its existence in non-saline drinking-water sources with pathogenic viability was unknown. A 'matched-pair-case-control' study identified the primary source of V. alginolyticus infection as 'tap-water' distributed by the municipality, used for drinking (MOR: 8.33; 95% CI 2.51-27.6) and household chores (MOR: 3.75; 95% CI 1.24-11.3). Cardinal toxin gene 'tdh' and other pathogenicity markers viz.tlh, vppC, toxR, VPI, T3SS1 and sxt were detected in V. alginolyticus isolates. Expression potential of the hemolytic genes are demonstrated by hemolysis assay and transcriptome analysis. Altogether 30.55% of isolates exhibited strong hemolytic potential in vitro. RT-PCR revealed uninterrupted virulence gene expression in outbreak strains under heat stress. Surprisingly, ~ 100% of V. alginolyticus from the outbreak focus were sensitive/partially sensitive to all group of antibiotics except β-lactums, carbapenem and quinolones. High drug-sensitivity suggested lack of previous human gut exposure and indicated a fresh dissemination from the environmental niche to the community domain. The maximum likelihood phylogeny depicted multiple clades in V. alginolyticus strains from Pan India sources. Isolated outbreak strains shared common ancestry with the strains from nearby riverine system, a source of 'drinking water' supplied to the affected community, confirming its environmental origin. V. alginolyticus, traditionally a fish-pathogen, is steadily gaining an emerging epidemiological relevance alongside other waterborne diarrheagenic bacteria and its 'thermotolerant' attribute poses additional threat under the canvas of climate change.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"81 12","pages":"443"},"PeriodicalIF":2.3,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142567779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Polyphasic Identification of Rhizomonospora bruguierae gen. nov., sp. nov., Isolated from Mangrove Rhizosphere Soil. 从红树林根瘤土壤中分离出的 Rhizomonospora bruguierae gen.
IF 2.3 3区 生物学
Current Microbiology Pub Date : 2024-11-03 DOI: 10.1007/s00284-024-03975-4
Jaewoo Yoon, Tomohiko Tamura
{"title":"Polyphasic Identification of Rhizomonospora bruguierae gen. nov., sp. nov., Isolated from Mangrove Rhizosphere Soil.","authors":"Jaewoo Yoon, Tomohiko Tamura","doi":"10.1007/s00284-024-03975-4","DOIUrl":"10.1007/s00284-024-03975-4","url":null,"abstract":"<p><p>A novel yellowish-white actinobacterial strain, designated as TS60-4C<sup>T</sup>, was isolated from the rhizosphere soil of the mangrove Bruguiera gymnorhiza (L.) Lam. in Okinawa, Japan, and was subjected to a polyphasic assessment. The strain could grow at 0-3.0% NaCl concentrations (w/v), pH 6.0-10.0, and 20-37 °C. The 16S rRNA gene sequences-based phylogeny showed that the novel isolate belongs to the family Micromonosporaceae and that it shared the highest sequence similarity (98.4%) with Micromonospora craniellae LHW63014<sup>T</sup>. The hydrolysate of cell-wall of strain TS60-4C<sup>T</sup> contained alanine, glutamic acid, glycine, and meso-A2pm. The acyl type of muramic acid was N-glycolyl. The main (> 10%) fatty acids were anteiso-C<sub>17:0</sub> (22.1%), iso-C<sub>16:0</sub> (17.8%), iso-C<sub>15:0</sub> (14.7%), and anteiso-C<sub>15:0</sub> (10.3%) and the predominant menaquinones of the isolate were MK-9(H<sub>6</sub>) and MK-9(H<sub>4</sub>). The polar lipids of the isolate were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, an unidentified aminolipid, and three unidentified lipids. The genome size of strain TS60-4C<sup>T</sup> was 6.7 Mbp with a DNA G + C content of 72.4%. The average nucleotide identity, digital DNA-DNA hybridization, and average amino acid identity value could differentiate strain TS60-4C<sup>T</sup> from its closely related taxa. The genome of the strain had 22 putative biosynthetic gene clusters for secondary metabolites, indicating a significant potential as a producer of bioactive chemicals. Consequently, the strain is considered to represent a novel genus and a new species of the family Micromonosporaceae, for which the name Rhizomonospora bruguierae gen. nov., sp. nov. is proposed. The type species is R. bruguierae, with the type strain TS60-4C<sup>T</sup> (= NBRC 107566<sup>T</sup> = TBRC 2024<sup>T</sup>).</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"81 12","pages":"440"},"PeriodicalIF":2.3,"publicationDate":"2024-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142563648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Deciphering Phyllomicrobiome of Cauliflower Leaf: Revelation by Metagenomic and Microbiological Analysis of Tolerant and Susceptible Genotypes Against Black Rot Disease. 解密花椰菜叶片的植物微生物组:通过对耐黑腐病基因型和易感基因型的元基因组和微生物学分析揭示花椰菜叶片微生物组。
IF 2.3 3区 生物学
Current Microbiology Pub Date : 2024-11-03 DOI: 10.1007/s00284-024-03969-2
Neelam Geat, Dinesh Singh, Partha Saha, Rajender Jatoth, Pedapudi Lokesh Babu, Gonur Somashekaraih Ramyashree Devi, Lalita Lakhran, Devendra Singh
{"title":"Deciphering Phyllomicrobiome of Cauliflower Leaf: Revelation by Metagenomic and Microbiological Analysis of Tolerant and Susceptible Genotypes Against Black Rot Disease.","authors":"Neelam Geat, Dinesh Singh, Partha Saha, Rajender Jatoth, Pedapudi Lokesh Babu, Gonur Somashekaraih Ramyashree Devi, Lalita Lakhran, Devendra Singh","doi":"10.1007/s00284-024-03969-2","DOIUrl":"10.1007/s00284-024-03969-2","url":null,"abstract":"<p><p>Understanding the phyllomicrobiome dynamics in cauliflower plants holds significant promise for enhancing crop resilience against black rot disease, caused by Xanthomonas campestris pv. campestris. In this study, the culturable microbiome and metagenomic profile of tolerant (BR-161) and susceptible (Pusa Sharad) cauliflower genotypes were investigated to elucidate microbial interactions associated with disease tolerance. Isolation of phyllospheric bacteria from asymptomatic and black rot disease symptomatic leaves of tolerant and susceptible cultivars yielded 46 diverse bacterial isolates. Molecular identification via 16S rRNA sequencing revealed differences in the diversity of microbial taxa between genotypes and health conditions. Metagenomic profiling using next-generation sequencing elucidated distinct microbial communities, with higher diversity observed in black rot disease symptomatic leaf of BR-161. Alpha and beta diversity indices highlighted differences in microbial community structure and composition between genotypes and health conditions. Taxonomic analysis revealed a core microbiome consisting of genera such as Xanthomonas, Psychrobacillus, Lactobacillus, and Pseudomonas across all the samples. Validation through microbiological methods confirmed the presence of these key genera. The findings provide novel insights into the phyllomicrobiome of black rot-tolerant and susceptible genotypes of cauliflower. Harnessing beneficial microbial communities identified in this study offers promising avenues for developing sustainable strategies to manage black rot disease and enhance cauliflower crop health and productivity.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"81 12","pages":"439"},"PeriodicalIF":2.3,"publicationDate":"2024-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142564160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Polluticaenibacter yanchengensis gen. nov., sp. nov., A Novel Taxon Within the Family Chitinophagaceae Isolated from Activated Sludge. Polluticaenibacter yanchengensis gen.
IF 2.3 3区 生物学
Current Microbiology Pub Date : 2024-11-02 DOI: 10.1007/s00284-024-03967-4
Zhuang Ke, Lin Wang, Ying Yang, Yingchun Du, Jian He, Cansheng Yuan
{"title":"Polluticaenibacter yanchengensis gen. nov., sp. nov., A Novel Taxon Within the Family Chitinophagaceae Isolated from Activated Sludge.","authors":"Zhuang Ke, Lin Wang, Ying Yang, Yingchun Du, Jian He, Cansheng Yuan","doi":"10.1007/s00284-024-03967-4","DOIUrl":"10.1007/s00284-024-03967-4","url":null,"abstract":"<p><p>A Gram-stain-negative bacterium, designated strain LY-5<sup> T</sup>, was isolated from an activated sludge sample collected from a river in Yancheng city, Jiangsu province, China. Cells of strain LY-5<sup> T</sup>, were strictly aerobic, non-motile and rod-shaped. Growth occurred at 15-37 °C (optimum, 30 °C), at pH 5.0-9.0 (optimum 7.0) and at 0-3% (w/v) NaCl (optimum, 0%). Phylogenetic analysis based on 16S rRNA gene and genome sequences indicated that strain LY-5<sup> T</sup> formed a distinct phyletic branch within the family Chitinophagaceae, with closest relatives being members of the genera Phnomibacter, Aurantisolimonas, and Chitinophaga, sharing 88.5-90.3% sequence similarity. Moreover, the average amino acid identity (AAI) between strain LY-5<sup> T</sup> and its closed phylogenetic neighbors was below 70%, indicating it belongs to a novel genus. The predominant cellular fatty acids of LY-5<sup> T</sup> were iso-C<sub>15:0</sub>, iso-C<sub>15:1</sub> G and summed feature 3 (C<sub>16:1</sub> ω7c and/or C<sub>16:1</sub> ω6c), and the only isoprenoid quinone was menaquinone-7 (MK-7). The major polar lipids identified in strain LY-5<sup> T</sup> comprised phosphatidylethanolamine, two unidentified aminolipids and five unidentified lipids. The genome size of strain LY-5<sup> T</sup> was 4.8 Mbp with a G + C content of 38.9%. Based on the evidence presented in this study, strain LY-5<sup> T</sup> represents a novel species of a new genus in the family Chitinophagaceae, for which the name Polluticaenibacter yanchengensis gen. nov., sp. nov. (Type strain LY-5<sup> T</sup> = CCTCC AB 2023260<sup> T</sup> = KCTC 102218<sup> T</sup>) is proposed.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"81 12","pages":"438"},"PeriodicalIF":2.3,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142563556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Toxicity, Antibacterial, Antioxidant, Antidiabetic, and DNA Cleavage Effects of Dextran-Graft-Polyacrylamide/Zinc Oxide Nanosystems. 葡聚糖接枝聚丙烯酰胺/氧化锌纳米系统的毒性、抗菌、抗氧化、抗糖尿病和 DNA 裂解效应
IF 2.3 3区 生物学
Current Microbiology Pub Date : 2024-11-02 DOI: 10.1007/s00284-024-03953-w
M Serkan Yalçın, Sadin Özdemir, Volodymyr Prokopiuk, Pavlo Virych, Anatolii Onishchenko, Gülşah Tollu, Vadim Pavlenko, Nataliya Kutsevol, Nadir Dizge, Anton Tkachenko, Kasim Ocakoglu
{"title":"Toxicity, Antibacterial, Antioxidant, Antidiabetic, and DNA Cleavage Effects of Dextran-Graft-Polyacrylamide/Zinc Oxide Nanosystems.","authors":"M Serkan Yalçın, Sadin Özdemir, Volodymyr Prokopiuk, Pavlo Virych, Anatolii Onishchenko, Gülşah Tollu, Vadim Pavlenko, Nataliya Kutsevol, Nadir Dizge, Anton Tkachenko, Kasim Ocakoglu","doi":"10.1007/s00284-024-03953-w","DOIUrl":"10.1007/s00284-024-03953-w","url":null,"abstract":"<p><p>Synthesis of metal oxide nanoparticles-polymer nanocomposites is an emerging strategy in nanotechnology to improve targeted delivery and reduce the toxicity of nanoparticles. In this study, we report biological effects of previously described hybrid nanocomposites containing dextran-graft-polyacrylamide/zinc oxide nanoparticles (D-PAA/ZnO NPs) prepared from zinc sulfate (D-PAA/ZnONPs(SO<sub>4</sub><sup>2-</sup>)) and zinc acetate (D-PAA/ZnONPs(-OAc)) focusing primarily on their antimicrobial activity. D-PAA/ZnONPs(SO<sub>4</sub><sup>2-</sup>) and D-PAA/ZnONPs(-OAc) nanosystems were tested in a complex way to assess their antioxidant activity (DPPH assay), antidiabetic potential (α-amylase inhibition), DNA cleavage activity, antimicrobial, and antibiofilm activity. In addition, the toxicity of D-PAA/ZnONPs(SO<sub>4</sub><sup>2-</sup>) and D-PAA/ZnONPs(-OAc) nanosystems against primary murine splenocytes was tested using MTT assay. The studied nanosystems inhibited E.coli growth. For all the investigated strains, minimum inhibitory concentrations (MICs) of D-PAA/ZnONPs(SO<sub>4</sub><sup>2-</sup>) and D-PAA/ZnONPs(-OAc) were in the range of 8 mg/L-128 mg/L and 16 mg/L-128 mg/L, respectively. The nanocomposites demonstrated effective antibiofilm properties as 94.27% and 86.43%. The compounds showed good antioxidant, anti-α-amylase, and DNA cleavage activities. D-PAA/ZnONPs(SO<sub>4</sub><sup>2-</sup>) and D-PAA/ZnONPs(-OAc) nanosystems reduced cell viability and promoted cell death of primary murine spleen cells at concentrations higher than those that proved to be antibacterial indicating the presence of therapeutic window. D-PAA/ZnONPs(SO<sub>4</sub><sup>2-</sup>) and D-PAA/ZnONPs(-OAc) nanosystems show antioxidant, antidiabetic, DNA cleavage, antimicrobial, and antibiofilm activity against the background of good biocompatibility suggesting the presence of therapeutic potential, which should be further investigated in vivo.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"81 12","pages":"437"},"PeriodicalIF":2.3,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142563649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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