Current Microbiology最新文献

{"title":"Aquimarina rhodophyticola sp. nov. and Aquimarina besae sp. nov., Isolated from Marine Red Algae.","authors":"Jeong Min Kim, Byeong Jun Choi, Hülya Bayburt, Dong Min Han, Che Ok Jeon","doi":"10.1007/s00284-025-04504-7","DOIUrl":"10.1007/s00284-025-04504-7","url":null,"abstract":"<p><p>Two Gram-negative, oxidase- and catalase-positive, non-motile, rod-shaped aerobic bacteria, designated strains W85ᵀ and SS2-1ᵀ, were isolated from marine red algae. Strain W85ᵀ grew optimally at 30 °C, pH 7.0, and 4.0% (w/v) NaCl, while strain SS2-1ᵀ grew optimally at 25-30 °C, pH 7.0, and 2.0-3.0% NaCl. Both strains contained menaquinone-6 as the sole respiratory quinone and shared major fatty acids, including iso-C_15:0, summed feature 9 (C_16:0 10-methyl and/or iso-C_17:1 ω9c), and iso-C_15:1 G, with iso-C_15:0 3-OH additionally present in strain W85ᵀ. The major polar lipids were phosphatidylethanolamine and diphosphatidylglycerol. The complete genomes of strains W85ᵀ (3.67 Mb, 3,151 genes) and SS2-1ᵀ (5.14 Mb, 4,564 genes) had a DNA G + C content of 34.1%. Genome annotation revealed that strain SS2-1ᵀ harbored more carbohydrate-active enzymes (129 genes) than W85ᵀ (60 genes), suggesting enhanced polysaccharide-degrading capacity. Ecological distribution analysis indicated that strains W85ᵀ and SS2-1ᵀ occupy distinct ecological niches, implying potential functional specialization. Both strains shared 95.0% 16S rRNA gene similarity, 71.9% average nucleotide identity (ANI), and 18.5% digital DNA-DNA hybridization (dDDH), all below species-level thresholds. Phylogenetic analyses based on 16S rRNA gene and whole-genome sequences confirmed that strains W85ᵀ and SS2-1ᵀ form distinct lineages within the genus Aquimarina. Furthermore, ANI and dDDH values between each strain and other type strains were also well below the species delineation thresholds. Based on phenotypic, chemotaxonomic, genomic, and ecological characteristics, strains W85ᵀ and SS2-1ᵀ are proposed as Aquimarina rhodophyticola sp. nov. (W85ᵀ = KACC 23858ᵀ = DSM 119937ᵀ) and Aquimarina besae sp. nov. (SS2-1ᵀ = KACC 23859ᵀ = DSM 119931ᵀ), respectively.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 11","pages":"504"},"PeriodicalIF":2.6,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145069364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering Autoantigen Signatures in Chikungunya Virus Infection Using Machine Learning: A Data-Driven Approach to Understand Host Immunity.","authors":"Chaitra Mallasandra Krishnappa, Shanker Govindaswamy, Anjali Ganjiwale","doi":"10.1007/s00284-025-04489-3","DOIUrl":"https://doi.org/10.1007/s00284-025-04489-3","url":null,"abstract":"<p><p>Chikungunya virus (CHIKV), a mosquito-borne alphavirus, causes acute febrile illness and often progresses to chronic arthritis-like symptoms for which the underlying molecular mechanisms remain elusive. This study identifies key biomarkers of CHIKV-host interactions, shedding light on potential mechanisms underlying virus-induced joint pathology. RNA sequencing data from peripheral blood samples of paediatric patients with natural Chikungunya infection (15-17 days post-symptom onset; GSE99992: Severe cases = 42, Non-Severe cases = 44) was analysed using binary classification models with StratifiedKFold validation, ensuring a robust and reliable approach to feature selection. A panel of 20 gene features selected by recursive feature elimination with cross-validation (RFECV) showed overlap with known autoantigens and were differentially expressed in CHIKV infection. Network analysis revealed interactions among host biomarkers-THG1L, SLC44A5, KCNN3-and viral components such as nsp4 (CHIKV RNA polymerase) and BCL2-like 11 (an apoptosis facilitator), highlighting a multifactorial virus-host interplay. Fibronectin 1 (FN1) emerged as a central hub gene, known for its role in osteoblast mineralization, skeletal development and its association with renal pathologies. These findings provide novel insights into CHIKV-induced immune dysregulation and offer a foundation for future experimental validation and therapeutic exploration.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 11","pages":"501"},"PeriodicalIF":2.6,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145069321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating the Impact of Cultivation Regimes on the Antibacterial Properties of Non-Polar Extracts of Chlorella sorokiniana and Anabaena sphaerica.","authors":"Mikhail S Temnov, Yana V Ustinskaya, Kirill I Meronyuk, Alexandra K Bryankina, Dmitry S Dvoretsky","doi":"10.1007/s00284-025-04479-5","DOIUrl":"10.1007/s00284-025-04479-5","url":null,"abstract":"<p><p>The study tested the hypothesis that cultivation conditions and additional exposure to light radiation influence the magnitude of the antibacterial effect of non-polar metabolites extracted from the cells of the microalgae Chlorella sorokiniana and the cyanobacteria Anabaena sphaerica. It was found that non-polar extracts of the microalgae Chlorella sorokiniana and the cyanobacteria Anabaena sphaerica extracted with petroleum ether have an inhibitory effect on the growth of Gram-positive bacteria of the genus Bacillus. The most promising antibacterial agents are extracts of microalgae and cyanobacteria obtained from cell biomass grown at the level of photosynthetically active radiation 100 ± 10 µmol photons/(m²s) and temperature - 36 ± 0.1 °C. At the same time, the non-polar extract of the microalgae Chlorella sorokiniana has an inhibitory effect only when the system is additionally exposed to light (100 ± 10 µmol photons/(m²·s)) (the minimum inhibitory concentration of the extract is ≈ 125 µg/ml), whereas the non-polar extract of the cyanobacteria Anabaena sphaerica shows antimicrobial activity both with and without additional light radiation (the minimum inhibitory concentration of the extract is 72 µg/ml and 830 µg/ml, respectively). For these extracts, IC₅₀ concentration values for the HEK293 cell line and SI selectivity indices for Chlorella sorokiniana (IC₅₀ = 260 µg/ml, SI ('light') = 2.08) and Anabaena sphaerica (IC₅₀ = 220 µg/ml, SI = 3.06/0.27 ('light'/'dark')) were determined.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 11","pages":"500"},"PeriodicalIF":2.6,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145052442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pseudomonas sp. MSW2-Mediated Biodegradation of Pharmaceutical Micropollutants: Experimental and In Silico Investigations.","authors":"Meghmala S Waghmode, Dipak K Sahoo, Neha N Patil, Pragati S Abhyankar, Dattatray D Gaikwad, Shamasoddin Shekh","doi":"10.1007/s00284-025-04486-6","DOIUrl":"10.1007/s00284-025-04486-6","url":null,"abstract":"<p><p>Environmental contamination from pharmaceutical and personal care products is a growing concern due to their widespread use. This study was aimed to investigate the biodegradation of acetaminophen and hydroxychloroquine alongside computational analysis (DFT calculations and molecular docking). The acetaminophen and hydroxychloroquine-tolerant strain was isolated from pharma industrial wastewater and identified as Pseudomonas sp. MSW 2 (GenBank: PP800223.1) based on morphological, biochemical, as well as DNA barcoding method. Based on the UV-VIS spectroscopy and HPLC data it was confirmed that Pseudomonas sp. MSW 2 degrades 1000 ppm of acetaminophen by 95% within 3 days and 50 ppm of hydroxychloroquine by 95% within 5 days, following first-order degradation kinetic models with rate constants of 0.65 d^-1 and 0.457 d^-1, respectively. Based on HRMS and ¹H NMR spectroscopy data, 1,4-benzoquinone and 7-chloro-4-quinolinamine were identified as degradative product of acetaminophen and hydroxychloroquine, respectively. The HOMO-LUMO energy gap (ΔEg) for acetaminophen,1,4 benzoquinone, hydroxychloroquine and 7-chloro-4-quinolinamine is 5.35 eV, 2.38 eV, 4.45 eV, and 4.55 eV, respectively. Data suggests that 1,4 benzoquinone has lower stability and higher reactivity compared to the acetaminophen. Whereas in case of hydroxychloroquine degradative product (7-chloro-4-quinolinamine), negligible changes were observed in the reactivity. Molecular docking simulations predicted a strong binding affinity (-26 kcal/mol) between acetaminophen and the amidase (PDB ID 2UXY) enzyme from P. aeruginosa, facilitated by hydrogen bonding. This study gives new insights in the bioremediation process, using the DFT calculations to theoretically document the reactivity and stability of pollutant as well as their biodegradative metabolites.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 11","pages":"499"},"PeriodicalIF":2.6,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145052456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pseudoduganella rhizocola sp. nov., Isolated from Rhizospheric Soil.","authors":"Shakiba S Chegini, Jaincy N Jayan, Yong Ho Kim, Sathiyaraj Srinivasan, Sang-Seob Lee","doi":"10.1007/s00284-025-04493-7","DOIUrl":"10.1007/s00284-025-04493-7","url":null,"abstract":"<p><p>A novel bacterial strain, SM-13^T was isolated from the rhizospheric soil of Epipremnum aureum (Jade Pothos) sampled in Suwon, Republic of Korea. The isolate was Gram-stain-negative, aerobic, motile, rod-shaped, cream-coloured, oxidase- and catalase-positive. Strain SM-13^T grew at the range of 15-37 °C (optimum, 25 °C), at pH 6.0-9.0 (optimum, pH 7.0), and in the presence of 0-0.5% (w/v) NaCl (optimum, 0%). Phylogenetic analysis based on genome and 16S rRNA gene sequences indicated that strain SM-13^T was affiliated with the family Oxalobacteriaceae and most closely related to Pseudoduganella humi THG S6-8^T (97.8%), Pseudoduganella violacea CAVIO^T (97.7%), Pseudoduganella buxea A9^T (97.6%), Pseudoduganella dura 16^T (97.5%), Pseudoduganella umbonata LP01^T (97.5%), and Pseudoduganella lurida D5^T (97.4%). The major ubiquinone was Q-8. The major identified polar lipids were phosphatidylethanolamine (PE), phosphatidylglycerol (PG), diphosphatidylglycerol (DPG), amino lipids (AL), and unknown lipids (L1, L2). The major fatty acids of SM-13^T were summed feature 3 (C_16:1 ω7c/C_16:1 ω6c) (51.9%) and C_16: 0 (18.7%). The novel strain SM-13^T has a draft genome size of 5,401,088 bp (27 contigs), annotated with 4,880 protein-coding genes, 59 tRNA and 2 rRNA genes. The genomic DNA G + C (%) content was 64. Hence, the strain SM-13^T represent a novel species within the genus Pseudoduganella, for which the name Pseudoduganella rhizocola sp. nov. is proposed. The Type strain is SM-13^T (= KCTC 8734^T = TBRC 19389^T = KEMB 24220^T). The GenBank/EMBL/DDBJ accession number for the complete 16S rRNA gene and genome sequence of the type strain SM-13^T is PQ198503 and JBJQIK000000000.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 10","pages":"498"},"PeriodicalIF":2.6,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145029344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Scorecard for Information Synthesis in Multiple Experimental Conditions: Application to Bacterial Biofilm Matrix Transcriptomics.","authors":"Mauro Nascimben, Lia Rimondini","doi":"10.1007/s00284-025-04435-3","DOIUrl":"10.1007/s00284-025-04435-3","url":null,"abstract":"<p><p>A Python-scripted software tool has been developed to help study the heterogeneity of gene changes, markedly or moderately expressed, when several experimental conditions are compared. The analysis workflow encloses a scorecard that groups genes based on relative fold-change and statistical significance, providing additional functions that facilitate knowledge extraction. The scorecard reports highlight unique patterns of gene regulation, such as genes whose expression is consistently up- or down-regulated across experiments, all of which are supported by graphs and summaries to characterize the dataset under investigation. Four GEO datasets related to RNA-seq bacterial biofilm expression levels were independently analyzed for information mining through the functionalities of the software library. The scorecard identified and tracked, over time or experiments, genes meaningful for bacterial metabolism and survival in response to antibiotics, adjuvants, and biocompatible materials. Analyses detected factors and strategies to persist in the environment by bacterial aggregates, such as modifications in the binding affinity of penicillin-related proteins or ribosomal subunits, the development of alternative metabolic pathways, cell wall thickening, intracellular concentration of drugs reduced by efflux pumps, and enzymatic inactivation through hydrolyzation, phosphorylation, or adenylation.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 10","pages":"497"},"PeriodicalIF":2.6,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12420718/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145023033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Niabella insulamsoli sp. nov., Isolated From Soil and Showing Potential Cosmetic Functions with Flexirubin Extract.","authors":"Nhi Thi Ai Nguyen, Jaisoo Kim","doi":"10.1007/s00284-025-04476-8","DOIUrl":"10.1007/s00284-025-04476-8","url":null,"abstract":"<p><p>A yellow-pigmented, non-motile, rod-shaped, and Gram-stain-negative bacterium was isolated from the soil of Yeongheung Island, Korea. The novel isolate, strain N803^T, was strictly aerobic, grew optimally at 30-35 °C, at pH 6.5, and in the presence of 0-2% NaCl. The closest phylogenetic relatives of strain N803^T are Niabella ginsengisoli GR10-1^T (97.6%), Niabella yanshanensis CCBAU 05354^T (96.2%), Niabella aquatica RP-2^T (96.2%), Niabella hibiscisoli THG-DN5.5^T (96.1%). The digital DNA-DNA hybridization (dDDH) and Average Nucleotide Identity (ANI) value between strain N803^T and nearest relatives N. gingsengisoli were 18.9 and 73.8%, respectively, which were below the 70% dDDH and 95% ANI for Niabella species denineation. The DNA G + C content was obtained from the whole-genome sequence was 44.6% and the principal respiratory quinone was MK-7. The predominant cellular fatty acids were iso-C15: 0, iso-C15: 1 G, C17:0 3OH, and summed feature 3 (C16:1 ω7c and/or C16:1 ω6c). The flexirubin pigment of strain showed antioxidant activity, tyrosinase inhibition and ultraviolet protective effects. On the basis of evidence from our polyphasic taxonomic study, strain N803^T was classified into a novel species in the genus Niabella, for which the name Niabella insulamsoli sp. nov., has been proposed. The type strain is N803^T (= KACC 23747^T = NBRC 116942^T).</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 10","pages":"495"},"PeriodicalIF":2.6,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145023059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phosphorylated Derivatives of Quaternary Ammonium Salts: Synthesis, X-Ray Crystal Structure, Antimicrobial Activity and Ecotoxicity.","authors":"Rustam R Davletshin, Polina A Kuryntseva, Aidar M Gayneev, Gulnaz Sh Galieva, Svetlana Yu Selivanovskaya, Natal'ya V Davletshina, Kamil A Ivshin, Daut R Islamov","doi":"10.1007/s00284-025-04480-y","DOIUrl":"10.1007/s00284-025-04480-y","url":null,"abstract":"<p><p>Phosphorylated structural analogs of Benzalkonium Chloride-diisopropoxyphosphorylmethane (dimethyldodecylammonium) bromide 1 (phosphorylated quaternary ammonium salt) and isopropoxyphosphorylmethane (dimethylalkylammonium) 2 (phosphorylated betaine) were synthesized. The structure of compound 1 was confirmed by single crystal X-ray diffraction study. The antibacterial, antifungal, and ecotoxicological profiles of the synthesized compounds were evaluated against aquatic organisms and flowering plants. Both compounds demonstrated comparable toxicity toward Paramecium caudatum (Protista) and Ceriodaphnia affinis (Cladocera), with EC₁₀ values of compound 1-0.62 g/L (P. caudatum), 0.13 g/L (C. affinis) and compound 2-0.90 g/L (P. caudatum), 0.08 g/L (C. affinis). These values indicate significantly lower toxicity compared to Benzalkonium chloride (BC) control (EC₁₀ = 110 μg/L for P. caudatum, 30 μg/L for C. affinis). Neither compound exhibited toxicity toward the green alga Chlorella vulgaris, whereas BC inhibited growth by 93%. However, both compounds 1 and 2 inhibited Raphanus sativus seed germination at 10.0 g/L, whereas BC exhibited only marginal phytotoxicity even at this high concentration (10 g/L). According to the disk diffusion method, compound 1 exhibited superior activity against all tested isolates Bacillus subtilis (Gram-positive), Pseudomonas fluorescens (Gram-negative) and Fusarium sp. (micromycete). Compound 2 showed activity only against B. subtilis and Fusarium sp. at 10.0 g/L. Compound 1 inhibited microbial growth significantly more effectively than BC across all three microorganisms, while compound 2 performance did not differ substantially from the control. Thus, the synthesized compound 1-demonstrates antimicrobial efficacy comparable to reference compounds (Miramistin, Benzalkonium Chloride) while exhibiting substantially lower ecotoxicity. This enhanced safety profile suggests its production and application would pose reduced environmental risks.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 10","pages":"496"},"PeriodicalIF":2.6,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145023105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gut Microbes and Inflammation: Their Role in Spinal Cord Injury Progression and Secondary Damage.","authors":"Jinwang Dong, Tao Xie, Chen Xu, Zhengwei Xu, Dingjun Hao, Liang Dong","doi":"10.1007/s00284-025-04469-7","DOIUrl":"10.1007/s00284-025-04469-7","url":null,"abstract":"<p><p>This review article describes recent research advances in the relationship between spinal cord injury (SCI) and the gut microbiota and each other's inflammatory response. SCI is a serious neurological disease that directly damages physiological function. Recent studies have shown that SCI significantly affected the composition and function of the gut microbiota, and even caused intestinal inflammation. The gut microbiota, a community of microorganisms inhabiting the intestinal tract, is closely related to the human health. Recent studies have indicated that the disordered gut microbiota was strongly associated with the progression of several diseases, including intestinal inflammation and SCI, in addition, some specific strains may be involved in the development of intestinal inflammation and secondary inflammatory after SCI. These strains promote intestinal inflammation through the production of inflammatory mediators and activation of immune cells. Intestinal inflammation, in turn, further exacerbate spinal cord pathology and secondary inflammatory. In summary, there is an intimate interaction between the intestinal microbiota, intestinal inflammation and SCI, secondary inflammation. An in-depth study of these interaction mechanisms presented here may aid in understanding of SCI occurrence and development and provide new ideas for future therapeutic strategies.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 10","pages":"494"},"PeriodicalIF":2.6,"publicationDate":"2025-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145014181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and Molecular Characterization of Three Staphylococcus pseudintermedius Strains from Dogs and Humans in Egypt.","authors":"Haitham Elaadli, Yassien Badr, May Raouf, Stephen A Kania, Ola K Elsakhawy, Hend Altaib, Mohamed A Abouelkhair","doi":"10.1007/s00284-025-04477-7","DOIUrl":"10.1007/s00284-025-04477-7","url":null,"abstract":"<p><p>Staphylococcus pseudintermedius is an opportunistic pathogen that is largely associated with canine hosts but is becoming more widely recognized as a zoonotic pathogen. Understanding its genetic and phenotypic properties, such as virulence factors and antimicrobial resistance (AMR) profiles, is critical for infection control and vaccine development. In this study, we isolated and molecularly characterized three S. pseudintermedius isolates from dogs (hereafter referred to as S. pseudintermedius D8) and humans (hereafter referred to as S. pseudintermedius H10 and S. pseudintermedius H11) in Egypt. All three isolates showed 100% sequence identity with the nuc gene of the S. pseudintermedius SP_11304-3A reference genome. Multilocus sequence typing (MLST) revealed novel sequence types (STs) in the three isolates. The AMR determinants varied substantially among the isolates. While the mecA gene was absent, blaZ was detected in the canine isolate, indicating beta-lactamase-mediated penicillin resistance. Additionally, tetK and tetM genes were found conferring tetracycline resistance in different isolates. Resistance genes for aminoglycosides, chloramphenicol, fusidic acid, macrolides, streptothricin, and trimethoprim were also identified. All isolates were positive for key virulence genes, including immune evasion (AdsA), coagulase (coa), immunoglobulin-binding protein (sbi/spsK), exfoliative toxin (speta), enterotoxins (se-int and siet), fibrinogen binding protein gene (fnbB), and two-component pore-forming leukocidin genes (lukF and lukS). The S. pseudintermedius H11 isolate uniquely harbored the neuraminidase gene (nanB), while none of the isolates contained the gene coding for immunoglobulin G binding protein (spsQ). These findings highlight the differences in virulence and antimicrobial resistance genes among these S. pseudintermedius isolates, underlining the need for global surveillance and molecular characterization of this pathogen.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 10","pages":"493"},"PeriodicalIF":2.6,"publicationDate":"2025-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12414057/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145006006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}