Niabella insulamsoli sp. nov., Isolated From Soil and Showing Potential Cosmetic Functions with Flexirubin Extract.

Abstract

A yellow-pigmented, non-motile, rod-shaped, and Gram-stain-negative bacterium was isolated from the soil of Yeongheung Island, Korea. The novel isolate, strain N803^T, was strictly aerobic, grew optimally at 30-35 °C, at pH 6.5, and in the presence of 0-2% NaCl. The closest phylogenetic relatives of strain N803^T are Niabella ginsengisoli GR10-1 ^T (97.6%), Niabella yanshanensis CCBAU 05354 ^T (96.2%), Niabella aquatica RP-2 ^T (96.2%), Niabella hibiscisoli THG-DN5.5 ^T (96.1%). The digital DNA-DNA hybridization (dDDH) and Average Nucleotide Identity (ANI) value between strain N803^T and nearest relatives N. gingsengisoli were 18.9 and 73.8%, respectively, which were below the 70% dDDH and 95% ANI for Niabella species denineation. The DNA G + C content was obtained from the whole-genome sequence was 44.6% and the principal respiratory quinone was MK-7. The predominant cellular fatty acids were iso-C15: 0, iso-C15: 1 G, C17:0 3OH, and summed feature 3 (C16:1 ω7c and/or C16:1 ω6c). The flexirubin pigment of strain showed antioxidant activity, tyrosinase inhibition and ultraviolet protective effects. On the basis of evidence from our polyphasic taxonomic study, strain N803^T was classified into a novel species in the genus Niabella, for which the name Niabella insulamsoli sp. nov., has been proposed. The type strain is N803^T (= KACC 23747 ^T = NBRC 116942 ^T).