Current Microbiology最新文献

Integrating Modified Fe₃O₄ Nanoparticles and Nisin with T4 Bacteriophage for Enhanced Biofilm Eradication.

IF 2.3 3区生物学

Current Microbiology Pub Date : 2025-04-08 DOI: 10.1007/s00284-025-04212-2

Pei Zhang, Huanggen Yang, Malik Taj Ahmad, Qi Zheng, Guochao Nie, Ayesha Ahmad, Muslim Raza, Saleem Raza

{"title":"Integrating Modified Fe3O4 Nanoparticles and Nisin with T4 Bacteriophage for Enhanced Biofilm Eradication.","authors":"Pei Zhang, Huanggen Yang, Malik Taj Ahmad, Qi Zheng, Guochao Nie, Ayesha Ahmad, Muslim Raza, Saleem Raza","doi":"10.1007/s00284-025-04212-2","DOIUrl":"https://doi.org/10.1007/s00284-025-04212-2","url":null,"abstract":"Biofilm formation presents significant challenges in healthcare, food processing, and water treatment, contributing to antibiotic resistance and persistent infections. Effective strategies to combat biofilm-associated infections are urgently needed. This study introduces a novel approach to biofilm removal by bio-functionalizing T4 bacteriophage with modified Fe3O4 nanoparticles (NPs) and Nisin, an antibacterial peptide, to form the Fe3O4-Phage-T4 + Nisin complex. The aim is to enhance antimicrobial efficacy and biofilm eradication. The Fe3O4-Phage-T4 + Nisin complex was synthesized by conjugating T4 bacteriophage with modified Fe3O4 NPs and Nisin. The antimicrobial and antibiofilm activity of the complex was evaluated against multidrug-resistant Pseudomonas aeruginosa strains (PA01 and PA14) using biofilm inhibition and eradication assays. Stability and efficacy were further tested across a pH range of 5 to 8. The Fe₃O₄-Phage-T4 + Nisin complex exhibited superior biofilm removal compared to its individual components. The integration of Nisin broadened the antibacterial spectrum, targeting both Gram-positive and Gram-negative bacteria, while the modified Fe₃O₄ NPs enhanced phage penetration and bacterial cell disruption. The complex demonstrated significant biofilm inhibition and eradication, addressing the challenge of biofilm-related antibiotic tolerance, which often necessitates high antibiotic doses. Additionally, it maintained stability and efficacy across varying pH conditions.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 6","pages":"237"},"PeriodicalIF":2.3,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143810806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pathogen Partnerships or Power Struggles? Pseudomonas aeruginosa, and Staphylococcus aureus Dynamics in Cystic Fibrosis.

IF 2.3 3区生物学

Current Microbiology Pub Date : 2025-04-08 DOI: 10.1007/s00284-025-04167-4

Neveen M Saleh, Esraa S F Sudan, Sara H Mohamed, Maram M El-Shahed, Reda E Hamouda, Ahmed Osama El-Gendy, Ahmed A Farag

{"title":"Pathogen Partnerships or Power Struggles? Pseudomonas aeruginosa, and Staphylococcus aureus Dynamics in Cystic Fibrosis.","authors":"Neveen M Saleh, Esraa S F Sudan, Sara H Mohamed, Maram M El-Shahed, Reda E Hamouda, Ahmed Osama El-Gendy, Ahmed A Farag","doi":"10.1007/s00284-025-04167-4","DOIUrl":"https://doi.org/10.1007/s00284-025-04167-4","url":null,"abstract":"Cystic fibrosis (CF) is a polymicrobial infection characterized by interactions among various bacterial species that affect one another's cohabitation. The investigation of interspecies interactions in dual infections is essential to understand their reaction in the environment better and assist in the development of treatment regimens and innovative disease control approaches. Our hypothesis posits that co-infection interactions promote the adaptation of Staphylococcus aureus and Pseudomonas aeruginosa, potentially leading to synergistic action. To explore this, we examined dual-species interactions in co-isolated pairs of these organisms from Egyptian CF patients using laboratory media and artificial sputum media (ASM). Based on demographic data, 82 collected bacterial isolates from single, dual, and triple cultures were identified from 50 enrolled patients. In the interaction of the pairs in mimic media, P. aeruginosa exo-products significantly enhanced the biofilm formation and growth of S. aureus. Conversely, S. aureus did not inhibit P. aeruginosa biofilm formation. Furthermore, the biofilm mode of dual-organism growth provides protection in the CF context, as bacterial biofilms can withstand much higher antimicrobial levels compared to planktonically grown bacteria. Additionally, key biofilm genes regulated by quorum sensing were differentially expressed in both species in an isolate-dependent manner, highlighting their significant role in coexistence dual-species biofilm coexistence. In conclusion, our study illuminates the competitive and cooperative interactions between these two pathogens, which impact their coexistence and encourage biofilm production. This, in turn, accelerates disease progression and compromises patient health.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 6","pages":"236"},"PeriodicalIF":2.3,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143810807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation, Screening, and Identification of Staphylococcus epidermidis with Effective Probiotic Attributes.

IF 2.3 3区生物学

Current Microbiology Pub Date : 2025-04-07 DOI: 10.1007/s00284-025-04206-0

Sonia Sharma, Nazim Uddin, Gurleen Kaur Sodhi, Abdurahman Hajinur Hirad, Nancy George, Gursharan Kaur, Swapnil Kajale, Rajinder Kaur, Jagdish Chandra Bose, Vagish Dwibedi

{"title":"Isolation, Screening, and Identification of Staphylococcus epidermidis with Effective Probiotic Attributes.","authors":"Sonia Sharma, Nazim Uddin, Gurleen Kaur Sodhi, Abdurahman Hajinur Hirad, Nancy George, Gursharan Kaur, Swapnil Kajale, Rajinder Kaur, Jagdish Chandra Bose, Vagish Dwibedi","doi":"10.1007/s00284-025-04206-0","DOIUrl":"https://doi.org/10.1007/s00284-025-04206-0","url":null,"abstract":"In this investigation, Staphylococcus epidermidis SAS1 strain was isolated and assessed for its probiotic, antioxidant, antimicrobial, and anti-cancerous properties. The strain demonstrated no gelatinase, DNase, or hemolytic activity, highlighting its safety for human use. Comprehensive tests were conducted to assess its probiotic potential, including its tolerance and survival under in-vitro gastric conditions, such as acidic pH and bile salts as well as antimicrobial potential. Furthermore, the CAE (crude antibacterial extract) extracted from S. epidermidis exhibited potent free radical scavenging activities against the different radicals such as super anion, 2,2-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS), nitrogen oxide, and hydroxyl. The SAS1 strain also exhibited ferric ion and cupric-reducing antioxidant power. The anti-cancerous potential of the strain was also determined by a 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay, which was carried out using the human colorectal adenocarcinoma cell line HT-29. An IC50 value of 8.30 ± 0.35 µg/mL and morphological changes in HT-29 cells, including apoptotic damage, cell shrinkage, and release of cellular content, depicted the anti-cancerous nature of the strain. The potent lipase inhibition activity of SAS1 may expand the scope of treating diseases associated with lipid metabolism. These findings underscore the efficacy in vitro efficacy of the S. epidermidis SAS1 strain as a probiotic, antioxidant, lipase inhibitor, and anti-cancerous agent, which can be further studied for potential treatment of colon cancer and other therapeutic applications.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 6","pages":"235"},"PeriodicalIF":2.3,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143794926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioactive Fraction of Streptomyces thinghirensis MSA1 Effectively Inhibits Biofilm Forming Clinically Significant AMR Pathogens.

IF 2.3 3区生物学

Current Microbiology Pub Date : 2025-04-06 DOI: 10.1007/s00284-025-04214-0

S Aifa Fathima, A Yaser Arafath, Ragothaman Prathiviraj, Saqib Hassan, George Seghal Kiran, Joseph Selvin

{"title":"Bioactive Fraction of Streptomyces thinghirensis MSA1 Effectively Inhibits Biofilm Forming Clinically Significant AMR Pathogens.","authors":"S Aifa Fathima, A Yaser Arafath, Ragothaman Prathiviraj, Saqib Hassan, George Seghal Kiran, Joseph Selvin","doi":"10.1007/s00284-025-04214-0","DOIUrl":"https://doi.org/10.1007/s00284-025-04214-0","url":null,"abstract":"The escalating threat of antibiotic-resistant microorganisms necessitate the discovery of novel antibacterial agents. This study explores the potential of marine-associated actinomycetes, focusing on Streptomyces thinghirensis MSA1, isolated from the marine sponge Callyspongia diffusa in Palk Bay, India, for its notable antibacterial properties. To optimize the production of bioactive compounds of S. thinghirensis MSA1, we established optimal growth conditions (30 °C, pH 7, 2% salinity, 9-day incubation) and utilized ISP4 medium for enhanced secondary metabolite production. The extracted compound, MSA1, was analyzed through FTIR and GCMS, identifying 20 biologically active components. MSA1 demonstrated potent antibacterial activity against significant pathogens, including Escherichia coli, Klebsiella pneumoniae, Salmonella typhi, Pseudomonas aeruginosa, and MRSA, alongside remarkable antioxidant and anti-biofilm properties. These findings highlight the potential of MSA1 as a promising candidate for developing treatments against antibiotic-resistant infections. This study acknowledges the preliminary nature of the findings and the necessity for further in vivo and clinical trials to fully ascertain the therapeutic potential of MSA1. This research opens avenues for novel antibacterial agents in the fight against antibiotic resistance, underscoring the value of marine biodiversity in medical science.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 6","pages":"234"},"PeriodicalIF":2.3,"publicationDate":"2025-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143794812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolating, Screening, and Optimizing Antioxidant Production of Endophytic Bacteria From Zingiber officinale Roscoe (Ginger).

IF 2.3 3区生物学

Current Microbiology Pub Date : 2025-04-05 DOI: 10.1007/s00284-025-04197-y

Trang Thi Xuan Dai, Tuan Thanh Chau, Linh Chi Tran, Ta Lam Tai

{"title":"Isolating, Screening, and Optimizing Antioxidant Production of Endophytic Bacteria From Zingiber officinale Roscoe (Ginger).","authors":"Trang Thi Xuan Dai, Tuan Thanh Chau, Linh Chi Tran, Ta Lam Tai","doi":"10.1007/s00284-025-04197-y","DOIUrl":"https://doi.org/10.1007/s00284-025-04197-y","url":null,"abstract":"Zingiber officinale Roscoe (ginger) is widely used as a herb in traditional cuisines and medicines. This study aimed to identify endophytic bacteria with high antioxidative potential in ginger plants and determine the optimum conditions for antioxidant fermentation from the strain. The bacteria from ginger were isolated and the growth conditions were surveyed. The secondary metabolites of the endophytes extracted using ethyl acetate were used to determine the total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activities. Six bacterial strains were selected for identification using 16S rRNA gene sequencing. Kosakonia sp. ZO-Rh4 exhibited the highest activity, and the fermentation conditions were optimized. The results showed that 32 endophytic strains isolated from ginger tissues exhibited the significant activity. The strains ZO-Rh4, ZO-L2, ZO-S7, ZO-S8, ZO-L7, and ZO-L8, revealed the highest ones, belonged to the genera Kosakonia, Pantoea, Enterobacter, and Klebsiella. Under optimum fermentation conditions of 32.58 °C, 71.49 h, 14.45 g/L glucose, and a pH of 7.58, the Kosakonia sp. ZO-Rh4 produced a TPC of 285.74 mg gallic acid equivalents/mL and TFC of144.49 mg quercetin equivalents/mL. Furthermore, the antioxidant activity significantly increased, as evidenced by the EC50 values for the 2,2-diphenyl-1-picrylhydrazyl scavenging activity, total antioxidant capacity, and reducing power assays. These findings highlight the potential of endophytes, particularly Kosakonia sp. ZO-Rh4 is a natural antioxidant with various applications.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 5","pages":"233"},"PeriodicalIF":2.3,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143788134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reclamation of Calcareous Sodic Soil by Brevibacterium sp. SOTI06, a Calcite Dissolving Bacteria. 一种溶解方解石的细菌 Brevibacterium sp.

IF 2.3 3区生物学

Current Microbiology Pub Date : 2025-04-04 DOI: 10.1007/s00284-025-04205-1

S M Tamilselvi, P M Brindhavani, Chitdeshwari Thiyagarajan, Sivakumar Uthandi

{"title":"Reclamation of Calcareous Sodic Soil by Brevibacterium sp. SOTI06, a Calcite Dissolving Bacteria.","authors":"S M Tamilselvi, P M Brindhavani, Chitdeshwari Thiyagarajan, Sivakumar Uthandi","doi":"10.1007/s00284-025-04205-1","DOIUrl":"https://doi.org/10.1007/s00284-025-04205-1","url":null,"abstract":"Soil degradation due to sodicity is a major constraint to agricultural development in arid and semi-arid regions. The accumulation of exchangeable Na+ ions affects soil physicochemical properties, which subsequently increases pH, thus reducing crop yield and nutrient availability. Several practices have been followed for revitalizing salt-affected soils, such as the addition of inorganic or organic amendments. Since most of these soils are calcareous (CaCO3) in nature, they can serve as a cationic source to release Ca2+ to replace the Na+ from the clay-complex. Though CaCO3 is poorly soluble, dissolution can be easily achieved by calcite dissolving bacteria. The present study aimed to evaluate the efficiency of Brevibacterium sp. SOTI06, in reclaiming calcareous sodic soil, along with organic and inorganic inputs through a soil incubation study. The reduction in pH, Na+, ESP (Exchangeable sodium percent), and free CaCO3 during the incubation period confirms the efficiency of amendments. The pH was drastically reduced from 9.10 to 8.20 in gypsum-applied soils. The combined effect of bacterium and press mud reduced higher rates of CaCO3 in soil (16.6 to 14.5%), with a rise of Ca2+ ions (23.5 to 28.7 meq 100 g soil-1). The mean calcite dissolution was higher in bioinoculant and gypsum-applied soils (9.04%). The SEM (Scanning Electron Microscopy) images confirmed the colonization and calcite dissolution potential of Brevibacterium sp. SOTI06 by pit formation in calcite stones. Hence, this study revealed that the combined application of bioinoculants with organic and inorganic amendments can effectively reclaim calcareous sodic soils.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 5","pages":"230"},"PeriodicalIF":2.3,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143779392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plant Growth-Promoting Pseudomonas sp. TR47 Ameliorates Pepper (Capsicum annuum L. var. conoides Mill) Growth and Tolerance to Salt Stress.

IF 2.3 3区生物学

Current Microbiology Pub Date : 2025-04-04 DOI: 10.1007/s00284-025-04217-x

Aissa Bakelli, Guendouz Dif, Nadjette Djemouai, Meriam Bouri, Fikrettin Şahin

{"title":"Plant Growth-Promoting Pseudomonas sp. TR47 Ameliorates Pepper (Capsicum annuum L. var. conoides Mill) Growth and Tolerance to Salt Stress.","authors":"Aissa Bakelli, Guendouz Dif, Nadjette Djemouai, Meriam Bouri, Fikrettin Şahin","doi":"10.1007/s00284-025-04217-x","DOIUrl":"https://doi.org/10.1007/s00284-025-04217-x","url":null,"abstract":"Pepper plants are susceptible to salt stress conditions, while rhizobacteria are considered auspicious factors for boosting plant growth. In this investigation, the Tamarix gallica L. rhizospheric bacterium TR47 was assessed for its capacity to promote growth under normal and saline conditions in the greenhouse. In addition, the genetic mechanisms were investigated through genome mining. Strain TR47 demonstrated numerous growth-promoting characteristics, such as the production of siderophores, phosphate and potassium solubilization, the ability to generate 1-aminocyclopropane-1-carboxylate deaminase as well as indole-3-acetic acid production. The inoculation of pepper seedlings by strain TR47 subjected to normal and salt conditions resulted in significantly increased germination rates compared to uninoculated seeds. Furthermore, the findings demonstrated that strain TR47 exhibited greater fresh and dry biomass values in comparison to the control group, specifically during salt stress conditions. All of these characteristics may enhance plant growth and development, contribute to salt tolerance, and stress mitigation. The genome sequence of TR47 that is most likely related to Pseudomonas plecoglossicida DSM 15088 T consists of a single circular chromosome that is about 5.55 megabase pairs in length and has a GC content of 61.8%. By doing a comprehensive analysis of the whole-genome sequence, genes linked to the promotion of plant development were identified. Our results open up exciting possibilities for the use of TR47 as a biostimulant that may enhance pepper plants under saline conditions, sparking interest in its potential future applications.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 5","pages":"231"},"PeriodicalIF":2.3,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143779299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integration of Transcriptomics and Metabolomics Reveals Metabolism Dysregulation in HIV-1-Infected Macrophages.

IF 2.3 3区生物学

Current Microbiology Pub Date : 2025-04-04 DOI: 10.1007/s00284-025-04204-2

Zongxiang Yuan, Chuye Mo, Yiwen Kang, Junhan Zhang, Fengyi Wang, Wudi Wei, Fang Qin, Shihui Huang, Junjun Jiang, Hao Liang, Li Ye

{"title":"Integration of Transcriptomics and Metabolomics Reveals Metabolism Dysregulation in HIV-1-Infected Macrophages.","authors":"Zongxiang Yuan, Chuye Mo, Yiwen Kang, Junhan Zhang, Fengyi Wang, Wudi Wei, Fang Qin, Shihui Huang, Junjun Jiang, Hao Liang, Li Ye","doi":"10.1007/s00284-025-04204-2","DOIUrl":"https://doi.org/10.1007/s00284-025-04204-2","url":null,"abstract":"HIV-1 infection leads to metabolic changes in macrophages, yet a comprehensive understanding of its pathogenesis remains limited. To address this, we integrated transcriptomic and metabolomic analyses to uncover intracellular metabolic alterations in HIV-1-infected macrophages. We identified differentially expressed genes (DEGs) using RNA-sequencing, while metabolomic profiling was performed with UHPLC-QE-MS. The integration of transcriptomics and metabolomics was achieved through \"Joint Pathway Analysis,\" and reverse transcription-quantitative PCR (RT-qPCR) was used to validate the identified pathways. Our transcriptomic analysis revealed a total of 890 DEGs, comprising 424 downregulated and 466 upregulated genes in macrophages infected with HIV-1. KEGG enrichment analysis highlighted the biosynthesis of amino acids and glycine, serine, and threonine metabolism as significantly enriched (P < 0.05). RT-qPCR results confirmed the expression of key genes, including PHGDH, PSAT1, PSPH, CBS, CTH, and AOC2, associated with these pathways. From the metabolomic analysis, we identified 60 differential metabolites, with glycerophospholipids representing the majority (51.67%). The integrated analysis revealed significant changes in glycine, serine, and threonine metabolism, glycerophospholipid metabolism, and linoleic acid metabolism in HIV-1-infected macrophages. This study offers an extensive overview of metabolic alterations in HIV-1-infected macrophages, which may enhance our understanding of the pathogenesis and highlight potential therapeutic targets.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 5","pages":"232"},"PeriodicalIF":2.3,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143779292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Elucidation of Foraging Strategies of Ganoderma lucidum and Ganoderma neo-japonicum on Different Nutrients Composition Using Fractal Morphology.

IF 2.3 3区生物学

Current Microbiology Pub Date : 2025-04-03 DOI: 10.1007/s00284-025-04207-z

Karisvini Ravi, Yee Shin Tan, Mohd Zahurin Mohamed Kamali, Sithi Vinayakam Muniandy

{"title":"Elucidation of Foraging Strategies of Ganoderma lucidum and Ganoderma neo-japonicum on Different Nutrients Composition Using Fractal Morphology.","authors":"Karisvini Ravi, Yee Shin Tan, Mohd Zahurin Mohamed Kamali, Sithi Vinayakam Muniandy","doi":"10.1007/s00284-025-04207-z","DOIUrl":"10.1007/s00284-025-04207-z","url":null,"abstract":"Ganoderma species are major wood-decaying fungus found everywhere due to its nutritional benefits. Challenges include connecting 2D imaging of mycelium growth morphology analysis to fractal morphology and determining optimum nutrient composition to increase production yield on a larger scale in mushroom industries. In this research, a comparative study of the growth dynamics of Ganoderma lucidum and Ganoderma neo-japonicum was conducted on different nutrients including potato dextrose agar (PDA), half-strength PDA and agarose using agar plate method. Macroscopic images were used to determine average growth front length, isotropy, area and perimeter fractal dimensions, and allometric scaling exponents. The result showed that G.lucidum mycelium recorded the fastest growth rate (10.51 ± 0.018 cm on 5th day) in PDA compared to other media. Dextrose (carbon source) is necessary for mycelial growth. Meanwhile, G.neo-japonicum mycelium showed similar growth rate on PDA and half-strength PDA. G.neo-japonicum was more aggressive and could thrive even on low nutrient concentration media. Box-counting approach is used to calculate perimeter fractal dimension DP and area fractal dimension DA. Mycelium with weak branching has fractal dimension closer to 1.0 (sparse line-like), while dense branching has fractal dimension closer to 2.0 (area filling). The allometry scaling exponent (σ) defined as the ratio of perimeter fractal dimension to area fractal dimension is used to describe mycelium morphology. Therefore, fractal and image analysis are crucial in studying evolving complex patterns within mycelium networks. The significance of the study contributes to the large-scale mushroom industry, aligning with Sustainable Development Goal 2 (SDG2).","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 5","pages":"229"},"PeriodicalIF":2.3,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143771717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacillus sp. Strain Fo03, a Phosphate Solubilizing Bacterial Strain, Promotes Potato Growth and Decrease Inorganic Fertilizer.

IF 2.3 3区生物学

Current Microbiology Pub Date : 2025-04-03 DOI: 10.1007/s00284-025-04198-x

Denisse M de la Paz-Osorio, Nadia de la Portilla-Lopez, Rocío Vaca-Paulín, Jorge Lugo-de la Fuente, Arnoldo Wong-Villarreal, Ivana F Della Mónica, Pedro Del Águila-Juárez, Martha E Mora-Herrera, Gustavo Yañez-Ocampo

{"title":"Bacillus sp. Strain Fo03, a Phosphate Solubilizing Bacterial Strain, Promotes Potato Growth and Decrease Inorganic Fertilizer.","authors":"Denisse M de la Paz-Osorio, Nadia de la Portilla-Lopez, Rocío Vaca-Paulín, Jorge Lugo-de la Fuente, Arnoldo Wong-Villarreal, Ivana F Della Mónica, Pedro Del Águila-Juárez, Martha E Mora-Herrera, Gustavo Yañez-Ocampo","doi":"10.1007/s00284-025-04198-x","DOIUrl":"10.1007/s00284-025-04198-x","url":null,"abstract":"Biofertilizers are a sustainable biotechnological alternative for decreasing use of inorganic fertilizer. Some bacterial strains, such as Bacillus pumilus, can solubilize phosphates, making them available to crops such as potato (Solanum tuberosum L. cv. Citlali). In this paper was studied the effect of Bacillus sp. strain Fo03, a phosphate solubilizer bacterial strain, on the growth promotion, production, and sprouting of potatoes under greenhouse conditions, compared with inorganic fertilizer. Five treatments were assayed: NPK 100% (T1), NK 100% (T2), Bacillus sp. strain Fo03, BP (T3), BP + NPK 50% (T4), and BP + NK 50% (T5). The parameters evaluated were plant height, fresh and dry weights, number of internodes, SPAD values, fresh weight of tubers, total number of tubers, number of tubers with diameters greater than or equal to 15 mm, length and width of sprouts. Treatments T1 and T4 showed a similarity in plant height (21.65 cm, 20.95 cm), number of internodes (12.42, 13.11), fresh weight (9.97 g, 5.53 g), and dry weight (1.87 g, 1.08 g), respectively. The tuber fresh weight (g), number of total tubers, tuber diameter ≥ 15 mm, and sprout width were statistically significant in T4 compared to the treatment with Bacillus sp. strain Fo03. The highlight result was the effect combined of Bacillus sp. strain Fo03 and the half dose of mineral fertilizer (NPK 50%) for having good yield of potato cultivation (growth, production, and sprouting), providing to farmers an alternative response for decreasing use of inorganic fertilizers. The use of Bacillus sp. strain Fo03 as biofertilizer may have a promising effect on the quality of potatoes, particularly in sprout length and width.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 5","pages":"228"},"PeriodicalIF":2.3,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143771705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0