Current Microbiology最新文献

Conjugative Transfer Potential of Antibiotic Resistance from Wastewater-derived Coliforms to riverine Escherichia coli. 废水大肠菌群对河流大肠杆菌抗生素耐药性的共轭转移势。

IF 2.8 3区生物学

Current Microbiology Pub Date : 2026-10-27 DOI: 10.1007/s00284-026-05199-0

Soichiro Tamai, Kohei Tsuda, Yoshihiro Suzuki

{"title":"Conjugative Transfer Potential of Antibiotic Resistance from Wastewater-derived Coliforms to riverine Escherichia coli.","authors":"Soichiro Tamai, Kohei Tsuda, Yoshihiro Suzuki","doi":"10.1007/s00284-026-05199-0","DOIUrl":"https://doi.org/10.1007/s00284-026-05199-0","url":null,"abstract":"Antibiotic-resistant bacteria (ARB) discharged into rivers from wastewater treatment plants disseminate antibiotic resistance genes (ARGs) through conjugative transfer, suggesting that aquatic environments serve as reservoirs for spreading antibiotic resistance. However, the actual extent of such dissemination of antibiotic resistance is unclear. This study investigated changes in the microbiota and antibiotic resistance profiles of other coliforms during the wastewater treatment process and evaluated whether other antibiotic-resistant coliforms persisting in treated wastewater could potentially transfer antibiotic resistance to Escherichia coli in receiving waters. The treatment process substantially reduced, but did not eliminate, E. coli and other coliforms (most probable number [MPN]/100 mL = 5 for E. coli and 4.3 × 10² for other coliforms), which were subsequently discharged into rivers. The resistance rates of other coliforms tended to increase throughout the treatment process, and the proportion of multidrug-resistant bacteria increased (influent: 4.9%, biologically treated water: 4.3%, effluent: 15.8%). Conjugation experiments using the filter mating method revealed the transfer of ARGs from Klebsiella species to E. coli (conjugation frequency: 10- 6-10- 3), suggesting the potential for antibiotic resistance transfer within biofilms. Experiments under simplified riverbed biofilm conditions confirmed the transfer of rifampicin resistance genes introduced into E. coli to antibiotic-resistant other coliforms (conjugation frequency: 9.7 × 10- 5-5.3 × 10- 4). The abundance of antibiotic-resistant other coliforms in the biofilm was 5.08 × 10² MPN/mL, which was lower than the observed conjugation frequencies, suggesting that the discharge area of treated wastewater is unlikely to function as a significant hotspot for ARB dissemination.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 12","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149285707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: A Novel Species Description of Sphingobium agrisoli sp. nov. 更正：Sphingobium agrisoli sp. nov.的新种描述。

IF 2.8 3区生物学

Current Microbiology Pub Date : 2026-10-24 DOI: 10.1007/s00284-026-05145-0

Xie-Feng Lu, Peng Gao, Shu-Yi Zhang, Yuan-Jia Xu, Guo-Hong Liu, Man Chen, Shun-Gui Zhou

引用次数: 0

The Potential Role of Postbiotics in Periodontitis Treatment and Preventive Dentistry. 后生物制剂在牙周炎治疗和预防性牙科中的潜在作用。

IF 2.8 3区生物学

Current Microbiology Pub Date : 2026-10-24 DOI: 10.1007/s00284-026-05179-4

Mitra Rostami, Mohammadreza Behnam Roudsari, Mina Abasi, Negar Khaneshi, Mohammad Gerayeli, Maede Yousefi, Abolghasem Amini, Ali Alizadeh, Fatemeh Fazel

{"title":"The Potential Role of Postbiotics in Periodontitis Treatment and Preventive Dentistry.","authors":"Mitra Rostami, Mohammadreza Behnam Roudsari, Mina Abasi, Negar Khaneshi, Mohammad Gerayeli, Maede Yousefi, Abolghasem Amini, Ali Alizadeh, Fatemeh Fazel","doi":"10.1007/s00284-026-05179-4","DOIUrl":"https://doi.org/10.1007/s00284-026-05179-4","url":null,"abstract":"Postbiotics, the bioactive molecules of probiotics and inactive microbial cells, have emerged as promising supplementary method in dental health treatment, specifically targeting disorders such as periodontitis. The definition, mechanisms of action and therapeutic potential of postbiotics in periodontitis are discussed in detail in this review. The capacity of postbiotics to control pathogenic bacteria development, increase immune responses and restore microbial balance, demonstrates the many roles of postbiotics in antimicrobial activity and anti-inflammatory effects in controlling the oral microbiome. Importantly, postbiotics have potential advantages over live probiotics such as greater stability and safety, without the need for microbial colonization. Postbiotics have shown potential in reducing gingival inflammation, as evidenced by clinical studies, thus offering a promising adjunct to conventional periodontal therapies. Current data, however, are limited, heterogeneous, and insufficient to support strong therapeutic claims. As research advances, the standardized addition of postbiotics to common dental care and hygiene products needs to be rigorously validated by large-scale clinical trials.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 12","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149264330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

β-defensin 130 Counteracts ETEC-K88 Infection in Mice: Mechanistic Insights from Gut Microbiome and LC-MS-Based Metabolomic Correlation Analysis. β-防御素130抑制小鼠ec - k88感染：来自肠道微生物组和lc - ms代谢组学相关分析的机制见解

IF 2.8 3区生物学

Current Microbiology Pub Date : 2026-10-24 DOI: 10.1007/s00284-026-05189-2

Zhiwei Su, Lili Han, Fengfeng Song, Yijie Wang, Yanjun Lin

{"title":"β-defensin 130 Counteracts ETEC-K88 Infection in Mice: Mechanistic Insights from Gut Microbiome and LC-MS-Based Metabolomic Correlation Analysis.","authors":"Zhiwei Su, Lili Han, Fengfeng Song, Yijie Wang, Yanjun Lin","doi":"10.1007/s00284-026-05189-2","DOIUrl":"https://doi.org/10.1007/s00284-026-05189-2","url":null,"abstract":"β-defensin 130 is a potential antimicrobial peptide candidate against enterotoxigenic Escherichia coli (ETEC)-K88 infection, but its in vivo mechanism remains unclear. In this study, the antibacterial activity of β-defensin 130 against ETEC-K88 was first evaluated in vitro, and membrane damage was observed by morphological disruption and PI uptake. Then, an ETEC-K88-infected mouse model was established and treated with β-defensin 130. Based on 16 S rDNA sequencing and LC-MS metabolomics, ETEC-K88 infection was shown to reduce gut microbial diversity and alter community structure. Correlation analysis revealed significant microbiota-metabolite interactions, β-defensin 130 treatment was associated with increased gut microbial diversity and partial recovery of the gut microbial community, increased beneficial Bacteroidota/Firmicutes, decreased pathogenic Proteobacteria, and partial reversal of infection-associated metabolic disturbances (e.g., upregulating L-tryptophan and linoleic acid). Collectively, these findings suggest that β-defensin 130 may alleviate ETEC-K88 infection by disrupting bacterial membranes, modulating gut microbiota, and partially restoring metabolic homeostasis, supporting its further development as a potential antimicrobial agent.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 12","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149264296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Palatine Tonsil Bacterial Microbiota in Pigs: Diverse, Underexplored, Yet Functionally Relevant. 猪的腭扁桃体细菌微生物群：多样化，未充分开发，但功能相关。

IF 2.8 3区生物学

Current Microbiology Pub Date : 2026-10-24 DOI: 10.1007/s00284-026-05184-7

Katell Hercouët, Florence Tardy, Corinne Marois-Créhan

{"title":"The Palatine Tonsil Bacterial Microbiota in Pigs: Diverse, Underexplored, Yet Functionally Relevant.","authors":"Katell Hercouët, Florence Tardy, Corinne Marois-Créhan","doi":"10.1007/s00284-026-05184-7","DOIUrl":"https://doi.org/10.1007/s00284-026-05184-7","url":null,"abstract":"The palatine tonsils play a pivotal role in porcine upper respiratory tract immunity by acting as an interface between the host and the external environment. As such, the tonsillar microbiota represents a critical yet insufficiently characterized microbial ecosystem. This review provides an overview of current knowledge on the porcine tonsillar microbiota, beginning with a description of sampling strategies and laboratory approaches, including metabarcoding, shotgun metagenomics, and culturomics. The development of the tonsillar microbiota over time and the main environmental, host-related, and management factors shaping its composition are examined. Particular emphasis is placed on the core bacterial communities commonly identified in the palatine tonsils, which are largely dominated by members of the Pasteurellaceae, Streptococcaceae, and Moraxellaceae families. The review further discusses how infectious and non-infectious diseases can disrupt tonsillar microbial homeostasis, as well as the effects of antimicrobial treatments, vaccination, and other prophylactic interventions on microbial structure and function. Finally, methodological biases inherent to current studies are highlighted, key knowledge gaps are identified, and future research directions are proposed to advance metagenomic investigations and better elucidate the role of the tonsillar microbiota in swine health and disease.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 12","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149264361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Characterization of Feline Leukemia Virus from Cats in Shelters in the Metropolitan Region of Recife, Pernambuco, Northeast Brazil. 巴西东北部伯南布哥省累西腓大都会区收容所里猫白血病病毒的分子特征

IF 2.8 3区生物学

Current Microbiology Pub Date : 2026-10-23 DOI: 10.1007/s00284-026-05191-8

Cláudia Kathariny da Silva Farias, Phelipe Magalhães Duarte, Wilson Jose da Silva Junior, Caio Andrey Bezerra Januário, Rita de Cássia Carvalho Maia, Otávio Valério de Carvalho, José Wilton Pinheiro Junior

{"title":"Molecular Characterization of Feline Leukemia Virus from Cats in Shelters in the Metropolitan Region of Recife, Pernambuco, Northeast Brazil.","authors":"Cláudia Kathariny da Silva Farias, Phelipe Magalhães Duarte, Wilson Jose da Silva Junior, Caio Andrey Bezerra Januário, Rita de Cássia Carvalho Maia, Otávio Valério de Carvalho, José Wilton Pinheiro Junior","doi":"10.1007/s00284-026-05191-8","DOIUrl":"10.1007/s00284-026-05191-8","url":null,"abstract":"This research aimed to conduct an epidemiological-molecular study of feline leukemia virus (FeLV) infection in cats from shelters in the Metropolitan Region of Recife (MRR), Pernambuco, Northeast Brazil. Blood samples from 260 cats from 18 shelters located in 5 municipalities within the MRR were analyzed. DNA samples from peripheral blood were subjected to Polymerase Chain Reaction (PCR) to detect FeLV proviral DNA. PCR-positive samples were sequenced and analyzed using BLAST software for phylogenetic tree construction. The prevalence of infection in cats was 9.6% (25/260). Among the 18 sampled shelters, 33.3% (6/18) had cats positive for FeLV. It was observed that 60% (3/5) of the investigated municipalities had felines positive for FeLV infection. Among the positive cats, males accounted for 52% (13/25) and females for 48% (12/25). Of the 22 sequenced samples, 18 sequences were used as queries for an online BLAST search to build a dataset. This study is pioneering in the state of Pernambuco and demonstrates the prevalence of FeLV infection in felines living in shelters in the Metropolitan Region of Recife, PE. Based on these results, we recommend vaccinating any cat with outdoor access in the study region. Understanding the prevalence of FeLV in Pernambuco provides insights into the local situation and helps develop effective hygienic-sanitary programs in feline shelters in the MRR, contributing to increased awareness of responsible pet ownership and reducing FeLV transmission among community felines.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 12","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13694291/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149243677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial Ethylene Glycol Metabolism: Pathways, Metabolic Engineering, and PET Upcycling. 微生物乙二醇代谢：途径，代谢工程，和PET升级回收。

IF 2.8 3区生物学

Current Microbiology Pub Date : 2026-10-23 DOI: 10.1007/s00284-026-05200-w

Jingzhen Qian, Pengju Wang, Weiqi Wang, Ju Li, Xin Zhang, Xingmiao Zhu, Ming Chen, Changhao Bi, Xueli Zhang

{"title":"Microbial Ethylene Glycol Metabolism: Pathways, Metabolic Engineering, and PET Upcycling.","authors":"Jingzhen Qian, Pengju Wang, Weiqi Wang, Ju Li, Xin Zhang, Xingmiao Zhu, Ming Chen, Changhao Bi, Xueli Zhang","doi":"10.1007/s00284-026-05200-w","DOIUrl":"10.1007/s00284-026-05200-w","url":null,"abstract":"Ethylene glycol (EG) is an attractive non-sugar carbon source because it can be obtained from petrochemical routes, renewable production platforms, and the depolymerization of polyethylene terephthalate (PET), thereby linking plastic upcycling with sustainable biomanufacturing. In recent years, substantial progress has been made in elucidating EG catabolism, including the initial oxidation of EG, native glyoxylate assimilation pathways, synthetic route design, and chassis engineering. Nevertheless, efficient and robust EG bioconversion is still limited by unfavorable thermodynamics, low catalytic efficiency, intermediate toxicity, and pathway-host incompatibility. In this Review, we summarize recent advances in three interconnected areas: the oxidation of EG to glycolaldehyde and its downstream processing; glyoxylate-centered native assimilation networks and their integration with central carbon metabolism; and synthetic pathways designed to improve carbon conservation and redirect carbon flux toward acetyl-CoA, central metabolites, and target products. We compare the tartronate semialdehyde pathway with the β-hydroxyaspartate cycle, discuss key design principles for synthetic modules, and highlight recent progress in chassis engineering, regulatory rewiring, and the direct conversion of PET hydrolysates. We further emphasize the ongoing transition from single-substrate EG utilization to integrated PET upcycling, in which mixed-monomer co-utilization, impurity tolerance, and aldehyde detoxification have emerged as major engineering targets. Overall, EG is becoming a promising platform substrate for connecting plastic waste recycling with green biomanufacturing, although further advances in enzyme discovery, pathway optimization, and pathway-host matching are still required.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 12","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149243701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Eugenol Activity Against Escherichia coli and Staphylococcus aureus: Biofilm Disruption and Additive Interactions with Antibiotics. 丁香酚抗大肠杆菌和金黄色葡萄球菌的活性：生物膜破坏和与抗生素的添加剂相互作用。

IF 2.8 3区生物学

Current Microbiology Pub Date : 2026-10-23 DOI: 10.1007/s00284-026-05201-9

Victor Alves Carneiro, Águida Maria Albuquerque Azevedo, Benise Ferreira da Silva, Ramaiana Soares Melo, Antônio Mateus Gomes Pereira, Rafaela Mesquita Bastos Cavalcante, Francisco Eduardo Aragão Catunda Junior

{"title":"Eugenol Activity Against Escherichia coli and Staphylococcus aureus: Biofilm Disruption and Additive Interactions with Antibiotics.","authors":"Victor Alves Carneiro, Águida Maria Albuquerque Azevedo, Benise Ferreira da Silva, Ramaiana Soares Melo, Antônio Mateus Gomes Pereira, Rafaela Mesquita Bastos Cavalcante, Francisco Eduardo Aragão Catunda Junior","doi":"10.1007/s00284-026-05201-9","DOIUrl":"10.1007/s00284-026-05201-9","url":null,"abstract":"The emergence of multidrug-resistant bacteria and their ability to form biofilms represent significant challenges in infection control. This study evaluated the antibacterial and antibiofilm activities of eugenol (Eug) against Escherichia coli and Staphylococcus aureus from food and clinical sources. Antibacterial activity was assessed using disk diffusion, broth microdilution (MIC/MBC), and bacterial growth kinetics. Antibiofilm activity was measured by crystal violet staining, CFU enumeration, and scanning electron microscopy (SEM). Checkerboard assays evaluated the combinatorial effect of Eug with ciprofloxacin and oxacillin. Eug exhibited inhibition zones of 13-22 mm and MIC/MBC values of 1000-2000 µg/mL. Subinhibitory concentrations significantly delayed bacterial growth. Biofilm biomass was reduced by up to 90% in E. coli and 80% in S. aureus, with SEM confirming structural alterations. Checkerboard assays revealed additive interactions with antibiotics. These findings provide additional evidence of Eug's antibacterial properties and support further investigation into its combinatorial effects with antibiotics against biofilm-associated challenges.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 12","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13694502/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149243714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Wavelength-Dependent Reduction of Newcastle Disease Virus Infectivity by Femtosecond Laser Irradiation: An In Ovo Proof-of-Concept Study. 用飞秒激光照射波长依赖性降低新城疫病病毒传染性：一项卵内概念验证研究。

IF 2.8 3区生物学

Current Microbiology Pub Date : 2026-10-23 DOI: 10.1007/s00284-026-05193-6

Amany Talaat El-Gendy, Salama A S Shany, Waleed M Arafa, Aya E Mohamed, Doaa M Abdellatif, Sabry M Tamam, Tarek Mohamed

{"title":"Wavelength-Dependent Reduction of Newcastle Disease Virus Infectivity by Femtosecond Laser Irradiation: An In Ovo Proof-of-Concept Study.","authors":"Amany Talaat El-Gendy, Salama A S Shany, Waleed M Arafa, Aya E Mohamed, Doaa M Abdellatif, Sabry M Tamam, Tarek Mohamed","doi":"10.1007/s00284-026-05193-6","DOIUrl":"10.1007/s00284-026-05193-6","url":null,"abstract":"Newcastle disease virus (NDV) causes substantial losses in poultry, and nonchemical approaches for reducing viral infectivity warrant investigation. Ultrafast femtosecond (fs) lasers offer a novel, non-thermal, and photosensitizer-free approach to pathogen inactivation. The primary aim of this study was to evaluate the wavelength-dependent efficacy of femtosecond laser irradiation (370-420 nm) in inactivating NDV, establishing a proof-of-concept for non-chemical viral attenuation using embryonated chicken eggs (ECEs) as a biological model. NDV suspensions were irradiated with 100 fs laser pulses at 370, 385, 400, and 420 nm for 60 min. The treated virus samples (100 µl) were inoculated into specific-pathogen-free (SPF) embryonated chicken eggs at dilutions of 10⁵, 10⁷ and 10⁹ and the viability of the embryo was monitored. Viral infectivity was assessed by embryo mortality and hemagglutination (HA) assays over 168 h. Exposure to 370 nm laser light resulted in > 99.99% reduction in viral infectivity and an estimated ≥ 4-log shift in the infectivity endpoint compared with the control, but complete inactivation was not observed. HA assays showed no protection at 385 and 400 nm on embryo death, but there was partial protection at 420 nm. Temperature monitoring did not show any thermal effects, indicating a non-thermal inactivation mechanism. This study demonstrates the strong antiviral potential of 370 nm femtosecond lasers against NDV; nevertheless, these findings justify further optimization of irradiation dose and independent confirmation by at least three serial passages. Viral antigen integrity, immunogenicity, and protective efficacy must be evaluated separately before any vaccine application.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 12","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149243722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differentially Expressed Infection-Associated Genes in a Shewanella algae isolate From a Deceased Northern Bottlenose Whale. 从死亡的北宽吻鲸分离的希瓦氏菌藻类中差异表达的感染相关基因。

IF 2.8 3区生物学

Current Microbiology Pub Date : 2026-10-22 DOI: 10.1007/s00284-026-05195-4

M H Eskildsen, S K Østergaard, S Menanteau-LeDouble, S Pagh, N de Jonge, J L Nielsen

{"title":"Differentially Expressed Infection-Associated Genes in a Shewanella algae isolate From a Deceased Northern Bottlenose Whale.","authors":"M H Eskildsen, S K Østergaard, S Menanteau-LeDouble, S Pagh, N de Jonge, J L Nielsen","doi":"10.1007/s00284-026-05195-4","DOIUrl":"10.1007/s00284-026-05195-4","url":null,"abstract":"Diseases are an important but poorly understood influence on marine ecosystems. However, many pathogens of wild marine organisms remain poorly characterized, limiting our understanding of their ecology and potential impacts. This study investigates the potential virulence mechanisms of a Shewanella algae strain isolated from the brain tissue of a deceased northern bottlenose whale (Hyperoodon ampullatus). Using long-read Oxford Nanopore RNA Sequencing, differential gene expression analysis (DGEA) was conducted comparing growth in standard media, Tryptic Soy Broth (TSB) and TSB supplemented with 5% brain homogenate (BH) to stimulate expression of genes potentially associated with virulence. Twenty-eight significantly differentially expressed genes (23 identified using the assembled genome and five additional non-overlapping genes detected using a closely related reference genome) were identified out of 7,213 genes with at least two primary mapped reads. These genes were associated with processes that have been linked to bacterial virulence in previous studies, including protein translocation (e.g., small signal recognition particle), flagellar motility, chemotaxis, and iron acquisition. Functional analysis indicated an increased expression of pathways related to amino acid metabolism and motility, whereas carbohydrate and inorganic ion transport were downregulated. These results suggest that environmental cues, potentially including factors related to iron metabolism, may influence bacterial virulence, obtained using Oxford Nanopore RNA sequencing for pathogen transcriptomics.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 12","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13690884/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149243683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0