{"title":"Current Progress and Future Perspective of Chlamydia trachomatis Infection: A Rising Threat to Women Health.","authors":"Vineeta Sharma, Mohd Mabood Khan","doi":"10.1007/s00284-025-04287-x","DOIUrl":"https://doi.org/10.1007/s00284-025-04287-x","url":null,"abstract":"<p><p>The most common sexually transmitted infection (STI) is caused by intracellular obligate bacteria Chlamydia trachomatis (CT) globally. Many Chlamydia infected women are asymptomatic or only experience minor signs, but others go on to develop salpingitis, endometritis, pelvic inflammatory disease (PID), ectopic pregnancy, and tubal factor infertility. It's been linked to both the spread of HIV and new infections, and it's suspected of playing a role in the emergence of cervical cancer. Clinically applicable rapid approaches for timely and precise identification of C. trachomatis infection would provide early treatment, hence reducing the risk of consequences. Traditional diagnostic methods Cell culture, direct immunofluorescence etc. are time-consuming but very sensitive assays for C. trachomatis infection. New and fast diagnostic method includes extraordinary optical transmission (EOT), loop-mediated isothermal amplification (LAMP), recombinase polymerase amplification (RPA), and microwave-accelerated metal-enhanced fluorescence (MAMEF) is more simple, rapid, sensitive, and cost-saving. This review provides fresh insight into our current understanding of C. trachomatis infection with emphasis on women's health. We outline a crucial summary of risk factors, molecular and clinical picture, prevention, diagnosis of C. trachomatis infection. Further, this review provides various modes of treatment, including available vaccination against genital C. trachomatis infection and the future directions to combat this sexually transmitted infection.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 7","pages":"314"},"PeriodicalIF":2.3,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144179735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Proposal to Transfer Bacillus yapensis to the Genus Robertmurraya as Robertmurraya yapensis comb. nov.","authors":"Zhouyan Dong, Jiangshan Hou, Syed Raziuddin Quadri, Ngoc Tung Quach, Manik Prabhu Narsing Rao, Yulong Wu","doi":"10.1007/s00284-025-04292-0","DOIUrl":"https://doi.org/10.1007/s00284-025-04292-0","url":null,"abstract":"<p><p>In the present study, the taxonomic position of Bacillus yapensis was evaluated using phylogenetic, amino acid identity (AAI) and average nucleotide identity (ANI) values. In the phylogenetic tree, Bacillus yapensis clustered with Robertmurraya species. The AAI value between Bacillus yapensis XXST-01<sup>T</sup> and Robertmurraya species was above the cut-off level (65%) for genus delineation. The ANI value between Bacillus yapensis XXST-01<sup>T</sup> and Robertmurraya species was below the threshold value (95-96%) for bacterial species delineation. Based on the results, we propose to transfer Bacillus yapensis to the genus Robertmurraya as Robertmurraya yapensis comb. nov. The type strain is XXST-01<sup>T</sup> (= MCCC 1A14143<sup>T</sup> = JCM 33181<sup>T</sup>).</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 7","pages":"312"},"PeriodicalIF":2.3,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144157473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nirbhay K Bhadani, Kritika Prasad, Nisha Gupta, Hemanta Sarmah, Tapas K Sengupta
{"title":"Salt-induced Reduction of Hyperswarming Motility in Bacillus cereus MHS is Associated with Reduction in Flagellation, Nanotube Formation and Quorum Sensing Regulator plcR.","authors":"Nirbhay K Bhadani, Kritika Prasad, Nisha Gupta, Hemanta Sarmah, Tapas K Sengupta","doi":"10.1007/s00284-025-04288-w","DOIUrl":"https://doi.org/10.1007/s00284-025-04288-w","url":null,"abstract":"<p><p>Bacteria have been known to thrive in challenging environmental niches through diverse phenomena. Swarming is one such favourable adaptations that could help bacteria survive extreme conditions. Therefore, targeting swarming is crucial for improving our understanding of bacterial motility and preventing related infections. Bacillus cereus, which causes food poisoning, has been shown to perform swarming, and salts like NaCl can act as a food preservative to control bacterial growth. To explore the possible alterations in the swarming of Bacillus cereus in the presence of salt, the present study encompasses the effect of NaCl on the swarming characteristics of a natural bacterial isolate, Bacillus cereus MHS, with a hyperswarming phenotype. Here we report that increased NaCl in growth media could induce a reduction in swarming motility and pattern of MHS on Luria agar plates. This observed reduction in swarming was found to be associated with reduced flagellation and a reduction in the abundance of bacterial nanotubes. Gene expression studies supported the phenotypic and ultrastructure observations as the expressions of bfla and ymdB genes, involved in formations of flagella and nanotubes, respectively, were found to be reduced in the swarming MHS cells in the presence of increased NaCl. It was also observed that the salt-induced reduction in swarming of MHS is associated with the reduced expression of the quorum sensing regulator gene plcR. This study first time reports the bacterial nanotubes in a Bacillus cereus strain indicating a possible link between the bacterial nanotube formation and hyperswarming phenotype in Bacillus cereus MHS.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 7","pages":"313"},"PeriodicalIF":2.3,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144173074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Bacteria in the Phylum Bacillota Degrade Nicotine Through the Variant of Pyridine and Pyrrolidine Pathway.","authors":"Xiu-Ming Liu, Xuan Hai, Cong-Jian Li, Yu-Fan Han, Xing Chen, Yu-Jin Fan, Jian Wu, En-Min Zhou, Yuan-Dong Li, Ling-Ling Yang, Yan-Qing Duan, Xiao-Yang Zhi","doi":"10.1007/s00284-025-04270-6","DOIUrl":"https://doi.org/10.1007/s00284-025-04270-6","url":null,"abstract":"<p><p>Nicotine is a harmful contaminant primarily derived from the waste produced by tobacco manufacturing facilities. Many bacterial strains have been reported to degrade nicotine, indicating their potential application in removing nicotine from sewage for wastewater treatment. The microbial degradation of nicotine is a green, economical, and efficient strategy, leading to a significant interest in discovering novel nicotine-degrading bacterial isolates. In this study, we isolated and identified 192 strains from tobacco leaves, roots, and rhizosphere soil. Nicotine utilization assays showed that 64 out of 192 strains had the potential capability of nicotine degradation, primarily within the genera Bacillus, Peribacillus, and Priestia in phylum Bacillota. Comparative genome analysis identified genes related to the variant of pyridine and pyrrolidine pathway scattered throughout the genomes of novel Bacillota strains. Phylogenetic analysis indicated that essential genes from these strains were evolutionarily distinct from others, forming a monophyletic clade. Our observations revealed that nicotine degradation-related genes have relatively independent origins and complex evolutionary histories in the phylum Bacillota.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 7","pages":"310"},"PeriodicalIF":2.3,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144149662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E Krivina, M Sinetova, A Starikov, O Anissimova, A Temraleeva
{"title":"Testing the Effectiveness of DNA Barcoding Markers and Species Delimitation Methods Within the Genus Coelastrella (Sphaeropleales, Chlorophyta), With a Description of Coelastrella polaris sp. nov. Isolated from Arctic Soils.","authors":"E Krivina, M Sinetova, A Starikov, O Anissimova, A Temraleeva","doi":"10.1007/s00284-025-04293-z","DOIUrl":"https://doi.org/10.1007/s00284-025-04293-z","url":null,"abstract":"<p><p>The species diversity in the genus Coelastrella is not yet fully unclarified, particularly with regard to algal communities in ecosystems characterized by extreme climatic conditions, such as those found in polar regions. The study objects were strains VKM Al-421, VKM Al-488, and VKM Al-489 isolated from the soils of the Far North, Russian Federation. The analysis of the 18S-ITS1-5.8S-ITS2 fragment revealed that these strains represent a unique phylogenetic lineage outside the 'core Coelastrella' group. The species status is also confirmed by morphological differences between the studied species and its sister species (absence of edges) (lack of ribs), interspecific genetic distances, the presence of compensatory base changes in the internal transcribed spacers ITS1 and ITS2, as well as the results of delimitation using various algorithms applied to both the full-length fragment and shorter barcodes. The strain VKM Al-421 of the new species exhibits a fatty acid profile characteristic of the genus Coelastrella; however, it differs from closely related strains due to the presence of Δ7,10,13-hexadecatrienoic acid and a notably high concentration of α-linolenic acid. These features may indicate an adaptation to polar regions. In addition, the studied strain has the potential to be used as a producer of α-linolenic acid, which is essential for human health. A detailed comparative analysis of the effectiveness of different DNA barcodes showed that ITS2 is the most promising for distinguishing species within Coelastrella. Among all species delimitation algorithms, GMYC is the most accurate when working with variable barcodes. At the same time, the less laborious KoT algorithm demonstrated a similar level of accuracy for ITS1.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 7","pages":"311"},"PeriodicalIF":2.3,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144149688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Polyphasic Taxonomic Analysis of Pontitalea aquivivens gen. nov., sp. nov., Isolated from Seawater.","authors":"Jaewoo Yoon","doi":"10.1007/s00284-025-04285-z","DOIUrl":"https://doi.org/10.1007/s00284-025-04285-z","url":null,"abstract":"<p><p>A Gram-stain-negative, rod-shaped, strictly aerobic, non-motile, and chemo-organoheterotrophic alphaproteobacterium, designated KMU-169<sup>T</sup>, was isolated from coastal seawater in the Republic of Korea. The novel isolate was able to grow at 0-2.0% NaCl concentrations (w/v), pH 6.0-9.5, and 15-40 °C. The analysis based on 16S rRNA gene sequences indicated that strain KMU-169<sup>T</sup> belongs to the family Paracoccaceae and shared the highest similarity (97.0%) with \"Defluviimonas salinarum\" CAU 1641<sup>T</sup>. The major (> 10%) cellular fatty acids were C<sub>18:1</sub> ω7c and summed feature 3 (comprising C<sub>16:1</sub> ω6c and/or C<sub>16:1</sub> ω7c). The predominant respiratory quinone was ubiquinone-10. Strain KMU-169<sup>T</sup> comprised phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine, an unidentified aminolipid, an unidentified phospholipid, and an unidentified lipid. The assembled draft genome size of strain KMU-169<sup>T</sup> was 4.1 Mbp with a DNA G + C content of 65.0%. The average nucleotide identity (ANI), average amino acid identity (AAI), and digital DNA-DNA hybridization (dDDH) values between the genomes of strain KMU-169<sup>T</sup> and its closely related taxa were 71.9-76.8%, 60.9-76.1%, and 18.4-21.0%, respectively. The genome of the strain KMU-169<sup>T</sup> revealed the presence of numerous genes involved in bio-macromolecule degradation, indicating a high potential for producing industrially valuable enzymes. Based on the polyphasic taxonomic data reported in this study, a novel genus and a new species of the family Paracoccaceae, for which the name Pontitalea aquivivens gen. nov., sp. nov., is proposed with the type strain KMU-169<sup>T</sup> (= KCCM 90598<sup>T</sup> = NBRC 117093<sup>T</sup>).</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 7","pages":"308"},"PeriodicalIF":2.3,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144149683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yifen Luo, Chengshao Zan, Mingsheng Chen, Fang Liu, Xiaohui Chen, Zhouqing Zheng, Xiao Ma, Li Tuo
{"title":"Consotaella aegiceratis sp. nov., a Novel Endophytic Bacterium Isolated from Root of Aegiceras corniculatum.","authors":"Yifen Luo, Chengshao Zan, Mingsheng Chen, Fang Liu, Xiaohui Chen, Zhouqing Zheng, Xiao Ma, Li Tuo","doi":"10.1007/s00284-025-04294-y","DOIUrl":"https://doi.org/10.1007/s00284-025-04294-y","url":null,"abstract":"<p><p>A Gram-stain-negative, aerobic, short rod-shaped and motile bacterium, designated strain CSK11QG-6<sup>T</sup> was isolated from root of Aegiceras corniculatum taken from Shankou Mangrove Ecological National Nature Reserve in Guangxi Zhuang Autonomous Region, China. Global alignment based on 16S rRNA gene sequences indicated that strain CSK11QG-6<sup>T</sup> shared the highest identity of 96.5% with Consotaella salsifontis USBA 369<sup>T</sup>. Phylogenetic analyses based on 16S rRNA gene sequences and genome sequences indicated that strain CSK11QG-6<sup>T</sup> clustered together with Consotaella salsifontis USBA 369<sup>T</sup>. Strain CSK11QG-6<sup>T</sup> had a genome size of 5.42 Mbp, with a N50 of 97.8 kb and coverage of 138 × . The DNA G + C content was 64.8%. The orthologous average nucleotide identity and digital DNA-DNA hybridization values of strain CSK11QG-6<sup>T</sup> with related species fell below the proposed species delineation threshold of 95-96% and 70%, respectively, indicating that strain CSK11QG-6<sup>T</sup> represented a novel species. Strain CSK11QG-6<sup>T</sup> contained C<sub>18:1</sub>ω7c and C<sub>16:0</sub> as the predominant fatty acids, and uniquinone-10 as the respiratory quinone. The major polar lipids were phosphatidylglycerol, phosphatidylcholine, diphosphatidylglycerol, phosphatidylethanolamine, an unidentified aminolipids and two unidentified phospholipids. Strain CSK11QG-6<sup>T</sup> exhibited chemical characteristics of fatty acids, lipids and quinones typical for the genus Consotaella, but could be clearly differentiated from Consotaella by many of phenotypic characteristics. Based on phylogenomic and phylogenetic analyses coupled with phenotypic and chemotaxonomic characterizations, strain CSK11QG-6<sup>T</sup> represents a novel species of the genus Consotaella, for which the name Consotaella aegiceratis sp. nov. is proposed. The type strain is CSK11QG-6<sup>T</sup> (= MCCC 1K07176<sup>T</sup> = JCM 35044<sup>T</sup>).</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 7","pages":"309"},"PeriodicalIF":2.3,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144149681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"EPS-Producing Bacteria Promote Aggregation in Soil Preventing the Leaching Loss of Nutrient.","authors":"Kapudeep Karmakar, Dipankar Roy, Srija Pal, Bratati Chowdhury, Ashok Choudhury","doi":"10.1007/s00284-025-04295-x","DOIUrl":"https://doi.org/10.1007/s00284-025-04295-x","url":null,"abstract":"<p><p>Leaching loss of nutrients from the agricultural field is a major concern in areas receiving excessive rainfall. The water-soluble nutrients are therefore lost due to leaching and runoff water. This leads to excessive dependence on chemical fertilizers. In this regard, the role of indigenous exopolysaccharide (EPS) producing bacteria in stabilizing the nutrient-holding capacity is far greater than conceived. The sub-Himalayan terai region, located at Cwa zone (monsoon type with dry winter, Köppen's classification) in India, which receives heavy rainfall (> 3000 mm), is a suitable location to study the role of such bacteria. A culture-dependent analysis of EPS-producing bacteria showed their comparatively lower abundance throughout the year. The EPS-associated bacterial microenvironment on vermiculite particles under aerobic and anaerobic conditions was visualized using a Confocal Laser Scanning Microscope (CLSM). The cells of EPS producers were found distributed within the EPS matrix, pushing each other away, showing a higher organic matter secretion (EPS) per unit of the bacterial cells. Since EPS has adhesive properties, the indigenous EPS-producing bacteria were amended to the soil, and the formation of macroaggregates was analyzed. Two in-house EPS-producing bacteria were used to monitor if they can reduce the leaching loss of nutrients. In vitro, assays on nutrient-holding capacity by soil-column-flow-through revealed that these bacteria-treated soil retained more nutrients. Till now, there were no reports on the role of the genus Serratia in improving the nutrient-holding capacity. For the first time, we have shown that using such soil-dwelling genera can improve the formation of stable aggregates and prevent nutrient loss.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 7","pages":"307"},"PeriodicalIF":2.3,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144141630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Exploring Escherichia coli EGY Type II L-Asparaginase Variant of Unique Glutaminase Activity: Cloning, Expression, Biochemical Characterization, and Molecular Docking Analysis.","authors":"Rawan Alshamy, Nehal Aly, Nefertiti El-Nikhely, Hisham A Nematalla, Mohamed Elkewedi, Hesham Saeed","doi":"10.1007/s00284-025-04233-x","DOIUrl":"https://doi.org/10.1007/s00284-025-04233-x","url":null,"abstract":"<p><p>Microbial L-asparaginase II is a cornerstone biopharmaceutical drug for the effective treatment of leukemia and other blood cancers. However, the associated cellular resistance mechanisms in response to the treatment require effective management and necessitate searching and developing of better versions of the enzyme. Overall, the current study described a new E. coli type II L-ASNase variant of unique L-glutaminase co-activity that was cloned into pET28a ( +) vector and expressed in E. coli BL21(DE3) pLysS as a cytosolic protein with a molecular weight of 38.390 kDa. The recombinant E. coli L-ASNase was purified by affinity chromatography and showed high specific activity of 7179.5 U/mg. The purified enzyme exhibited enormous thermal stability and its affinity for L-asparagine (L-Asn) and L-glutamine (L-Gln) was in silico analyzed by molecular docking and experimentally verified which indicated relatively similar affinity. The purified E. coli L-ASNase demonstrated high dual activities toward L-Asn (Km and Vmax 0.627 mM and 385 µmol min<sup>-1</sup>) with 90% specificity toward L-Gln (Km and Vmax 0.715 mM and 376 µmol min<sup>-1</sup>). The molecular docking study suggested that E. coli EGY L-ASNase follows a single displacement mechanism of catalysis based on the interacting residues analysis from a computational insight. Biochemical characterization and immunogenicity prediction were carried out to contribute for better understanding of the immunogenic determinants along with the other features of the new variant to consider its potentiality for in vitro and in vivo cytotoxicity testing in future studies.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 7","pages":"306"},"PeriodicalIF":2.3,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144141569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Naringenin as a Potent Natural Biofilm Inhibitor of Pseudomonas aeruginosa in Diabetic Foot Ulcers Through lasR Competitive Inhibition.","authors":"Anjali Warrier, Kapaettu Satyamoorthy, Thokur Sreepathy Murali","doi":"10.1007/s00284-025-04283-1","DOIUrl":"https://doi.org/10.1007/s00284-025-04283-1","url":null,"abstract":"<p><p>Chronic non-healing foot ulcers are a major complication in diabetic patients, contributing to significant morbidity and mortality. Microorganisms in these wounds form biofilms, conferring greater virulence and enhanced protection from antibiotics. Hence, we examined naringenin, and other natural compounds like chlorofuranone, 4-nitropyridine N-oxide, and quercetin as a positive control against the major pathogenic organism that forms biofilm in foot ulcers. Here, we focused on Pseudomonas aeruginosa, which produces high levels of biofilm in diabetic foot ulcers. Naringenin (47.10 µg/ml for PA21; 124.7 µg/ml for PA333) and other natural compounds were tested for their ability to inhibit biofilm formation and virulence in vitro, and their effect on biofilm-associated gene expression was studied. The biofilm inhibitory mechanism of naringenin was elucidated using in silico analysis and in vitro reporter gene assay. In vitro biofilm assays, confocal and scanning electron microscopy showed that natural compounds effectively inhibited biofilm, without causing cell death. Treatment with these compounds significantly altered the expression of genes associated with quorum sensing in P. aeruginosa, such as lasR, pslA, algA, gacS, and pelA. Naringenin decreased the production of major virulence factors in P. aeruginosa. Molecular docking showed that naringenin exhibited the strongest binding affinity to LasR, and the same was validated by reporter gene assay using plasmid pSB1142 indicating its role as a competitive inhibitor in the las quorum sensing system in P. aeruginosa. The findings of this study could be extrapolated to in vivo diabetic wound infection models to help optimize the use of naringenin in effective biofilm control for better wound management in diabetic patients.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 7","pages":"305"},"PeriodicalIF":2.3,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144141698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}