Current Microbiology最新文献

{"title":"Subinhibitory Concentrations of Oxacillin Alter Motility, Biofilm Production, and Transformation Capability of the Oxacillinase-Producing Acinetobacter baumannii.","authors":"Frida Guerrero-López, Yaraymi Ortiz, José Ángel Merino-Mascorro, Eduardo Franco-Frías, Santos García, Norma Heredia","doi":"10.1007/s00284-025-04542-1","DOIUrl":"https://doi.org/10.1007/s00284-025-04542-1","url":null,"abstract":"<p><p>Subinhibitory concentrations of various antibiotics can exacerbate microbial virulence. Acinetobacter baumannii is often resistant to oxacillin; however, the effect of low oxacillin concentrations on oxacillinase-producing bacteria remains unclear. Herein, oxacillinase producer A. baumannii ATCC-strains 2093 (motile) and 19606 (non-motile) were pre-exposed to sub-bactericidal concentrations (subMBC) of oxacillin (0.25, 0.5, and 1.0 mg/mL) and incubated at 37 °C. Growth kinetics, twitching and swarming-like motility, biofilm formation, exopolysaccharide production, transformation capability, and gene expression were determined. All oxacillin subMBC conditions exerted bacteriostatic effects in both strains. Pre-exposing the motile strain with 0.25 and 0.5 mg/mL of oxacillin for 1 h increased the twitching motility (4.2 ± 0.3 cm; control = 3.5 cm), whereas pre-exposure for 2 h it increased swarming-like motility (2.95 ± 0.15 cm; control = 2.7 cm). This strain also increased the biofilm production by effect of all 6 h-oxacillin subMBC treated bacteria (≤ 1.42 Biofilm Formation Index (BFI); control = 0.6 BFI), whereas the strain 19606 reduced biofilm up to 1 BFI (control = 2 BFI). The antibiotic also reduced the exopolysaccharide production in almost all treated cells from both strains. The transformation efficiency (TE) of strain 19606 increased X̄ = 19 ± 11% more than the control by effect of all DNA and oxacillin conditions studied; however, the TE for strain 2093 was lower than the control. The expression of genes for resistance (bla-OXA), twitching (pilA/tonB) and swarming-like motilities (dat/ddc), and biofilm production (csuE) were altered by the oxacillin subMBC. Overall, exposure of oxacillinase-producing A. baumannii to oxacillin subMBC increases several virulence factors, representing a potential public health risk.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 12","pages":"562"},"PeriodicalIF":2.6,"publicationDate":"2025-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145307215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing Plant Essential Oils to Combat Acinetobacter baumannii: Research Progress in Antimicrobial Mechanisms and Therapeutic Prospects.","authors":"Shu-Yun Wei, Yu Sun, Qing Lu, Zhen Liang, Tao Zhu, Yu-Long Li, Ding-Hui Xu, Cai-Ying Li, Feng-Lan Feng, Hong Zeng","doi":"10.1007/s00284-025-04533-2","DOIUrl":"https://doi.org/10.1007/s00284-025-04533-2","url":null,"abstract":"<p><p>Acinetobacter baumannii, a priority pathogen designated by the World Health Organization, poses a severe threat to global healthcare due to its widespread multidrug resistance (MDR) and ability to form persistent biofilms. Plant essential oils (EOs) have emerged as promising natural antimicrobials, but existing reviews lack systematic integration of their antimicrobial mechanisms, safety profiles, and clinical translational potential-especially in combination with advanced delivery systems. Work Plan: This review focuses on plant EOs from different families and genera, and conducts a systematic analysis from three core dimensions:(1) Antimicrobial activity dimension: It compares the differences in minimum inhibitory concentrations (MICs) of EOs from various families and genera against A. baumannii to clarify the strength of their antimicrobial efficacy; (2) Mechanism of action dimension: It analyzes the dual-level effects of bacterial intervention and host regulation. At the bacterial level, it focuses on the intervention effects on key targets such as cell membranes, biofilms, efflux pumps, and quorum sensing (QS); at the host level, it investigates the immunomodulatory effects in infection models; (3) Clinical potential dimension: It evaluates the translational value of these EOs, including their safety profiles, synergistic effects with antibiotics, and compatibility with advanced delivery systems. Innovation & Significance: This review is the first to construct an integrated framework covering EO antimicrobial mechanisms, host immunomodulation, and clinical priority ranking. Via multi-dimensional analysis, it fully explores EOs' potential against A. baumannii. The study provides actionable technical guidance for reducing this bacterium's resistance and developing green antimicrobial strategies.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 12","pages":"560"},"PeriodicalIF":2.6,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145307188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diversity and Antimicrobial Activity of Potential Endophytic Fungi Isolated from the Medicinal Plant Gmelina arborea.","authors":"Sisi Liu, Yuan Gao, Shaohua Wu","doi":"10.1007/s00284-025-04555-w","DOIUrl":"https://doi.org/10.1007/s00284-025-04555-w","url":null,"abstract":"<p><p>Gmelina arborea, a medicinal plant belonging to the Verbenaceae family, is extensively utilized in Ayurveda, the traditional Indian medical system. Endophytic fungi, which engage in mutualistic symbiosis with host plants, are recognized as significant microbial resources due to their ability to produce a diverse array of bioactive compounds. In this study, 131 potential endophytic fungal isolates were obtained from the roots, stems, leaves, and flowers of G. arborea. Phylogenetic analysis classified these fungi into 3 phyla, 6 classes, 13 orders, 23 families, and 27 genera. Diversity analysis indicated significant variations in isolation frequency among different tissues, with the highest frequency observed in roots, followed by flowers, leaves, and stems. The dominant genera identified were Diaporthe (17.6%), Fusarium (12.2%), and Mucor (12.2%). Antimicrobial screening against seven pathogenic microorganisms demonstrated that 23 potential endophytic strains (17.6%) exhibited significant inhibitory activity. These findings establish a foundation for further investigation into the antagonistic capabilities of potential endophytic fungi from G. arborea against pathogens and provide valuable insights into their diversity.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 12","pages":"561"},"PeriodicalIF":2.6,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145307213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the Metabolites and Biological Characteristics of Swampomyces armeniacus from Red Sea Mangroves Ecosystems.","authors":"Mohamed S Hodhod, Hager A Bendary, Mai M El-Ashmony, Monerah S M Alqahtani, Nouran M Hamouda, Ahmed Gomaa, Jawaher I AlAhadeb, Abdel-Rhman Z Gaafar, Sameh M H Khalaf","doi":"10.1007/s00284-025-04423-7","DOIUrl":"https://doi.org/10.1007/s00284-025-04423-7","url":null,"abstract":"<p><p>Obligate marine fungi associated with mangroves are a unique group that fulfils significant ecological functions in mangrove ecosystems. The present study aimed to assess the antimicrobial, antioxidant, and anticancer potentials of the marine fungus \"Swampomyces armeniacus\" isolated and characterized (on both morphological and molecular levels) from mangroves located in RAS Mohamed natural protectorate on the Red Sea coast; along with determining the metabolomics profiling of the candidate species. The antimicrobial capability was assessed via the agar well diffusion method against 6 strains (Bacillus cereus, Staphylococcus aureus, Shigella sonnei, Pseudomonas aeruginosa, Candida albicans, and Aspergillus niger) alongside determining the minimum inhibitory concentration (MIC) by two-fold microdilution method. The study findings indicated that S. armeniacus crude extract had antimicrobial activity against all test strains whereas the strongest effect was against B. cereus with MIC value 0.7 μg/ml. Moreover, the antioxidant potential was determined via DPPH which showed strong radical scavenging activity of 88.3% and IC50 = 20.4 μg/mL. Additionally, the extract exhibited significant anticancer activity against A549 lung cancer cells, with an IC50 value of 15.57 ± 0.27 µg/mL, while showing minimal toxicity toward normal Wi38 cells (IC50 = 37.71 ± 0.31 µg/mL). GC-MS investigation of S. armeniacus crude extract exhibiting antimicrobial, antioxidant, and anticancer related bioactive compounds such as (Di-tert-butylhydroquinone, 4-Aminocyclohexanecarboxylic acid) and various fatty acids namely (stearic acid, palmitic acid, Ethanedioic acid, and Butanedioic acid). These results highlight the biological activity of S. armeniacus and emphasizing its potential as a unique source of bioactive molecules for biotechnological and therapeutic applications.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 12","pages":"558"},"PeriodicalIF":2.6,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145285843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antifungal Activity and Mechanism of the Novel Antimicrobial Peptide Jelleine-Ic Against Candida albicans.","authors":"Lifang Wang, Ke Li, Jie Zhu, Kang Ding, Xiaomin Tao, Xiaomei Xu, Yan Zhu","doi":"10.1007/s00284-025-04551-0","DOIUrl":"https://doi.org/10.1007/s00284-025-04551-0","url":null,"abstract":"<p><p>Candida albicans, an opportunistic fungus, is the most common cause of invasive candidiasis globally. Drug resistance and limited number of antifungal compounds highlight the necessity of new antifungal drugs to treat C. albicans infections. Antimicrobial peptides (AMPs) exhibit potential as antifungal agents. The novel AMP Jelleine-Ic designed by our group demonstrated superior antibacterial activity than its parent peptide Jelleine-I. This work investigated the antifungal activity, antibiofilm activity and mechanism of action of Jelleine-Ic against C. albicans. Jelleine-Ic showed stronger antifungal activity against C. albicans than Jelleine-I. It effectively inhibited biofilm formation and destructed the preformed biofilm of C. albicans. Notably, it exhibited synergistic effect when combined with Amphotericin B. At concentrations below 125 μg/mL, it was not hemolytic on rabbit red blood cells. Jelleine-Ic compromised cell wall integrity, increased the permeability of C. albicans cell membrane. Furthermore, upon entering the cell, Jelleine-Ic bound with DNA, altered the expression of genes involved in DNA replication and repair, and induced the production of intracellular reactive-oxygen species. Jelleine-Ic increased the survival of Galleria mellonella infected with C. albicans. Collectively, Jelleine-Ic effectively controlled C. albicans, and could be a potential replacement for antibiotics and other chemical fungicides in the treatment of fungal infections.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 12","pages":"559"},"PeriodicalIF":2.6,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145285836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phenotypic Characterization of Null Mutants of ΔcbpA and ΔcbpB in S. enterica Serovar Typhimurium.","authors":"Indu Pant, Shashidhar Ravindranath","doi":"10.1007/s00284-025-04541-2","DOIUrl":"10.1007/s00284-025-04541-2","url":null,"abstract":"<p><p>The phenotypic characteristics of the ΔcbpA and ΔcbpB mutants of Salmonella enterica serovar Typhimurium were comprehensively analyzed to elucidate the roles of these nucleoid-associated proteins in metabolism and stress responses. Both ΔcbpA and ΔcbpB mutants exhibited enhanced growth on carbon sources such as ribose, mannose, fructose, glycerol, galactose and sucrose compared to the wild-type strain. Conversely, the wild-type strain demonstrated superior growth on xylose. Notably, the ΔcbpB mutant experienced a prolonged lag phase of ~ 20 h when cultured in M9 medium supplemented with maltose. In M9 medium where alanine or arginine served as the sole nitrogen source, both mutants outperformed the wild-type strain in growth, indicating potential alterations in nitrogen metabolism pathways. The ΔcbpA and ΔcbpB mutants were non-motile and exhibited reduced cell sizes compared to the wild-type. Specifically, the ΔcbpA mutant was approximately 0.8 times smaller than the size of the wild-type, while the ΔcbpB mutant was about 1.3 times smaller. The D₁₀ value, representing the radiation dose required to reduce the bacterial population by 90%, was 113 Gy for the wild-type, 140 Gy for ΔcbpA and 104 Gy for ΔcbpB, suggesting differential radiation sensitivities among the strains. Upon heat exposure, the ΔcbpA mutant exhibited a fivefold reduction in viable cell number compared to the wild-type and ΔcbpB, indicating increased heat sensitivity. No significant differences were observed among the strains in response to oxidative stress induced by hydrogen peroxide. After 72 h in 15% salt conditions, the wild-type strain showed a fivefold reduction in cell numbers, whereas both mutants exhibited only a fourfold reduction, suggesting enhanced salt tolerance. These findings underscore the pivotal roles of CbpA and CbpB proteins in regulating metabolic pathways and mediating stress responses in S. Typhimurium.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 12","pages":"557"},"PeriodicalIF":2.6,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12521295/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145285848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro and In Vivo Antimicrobial Activity of Melia Azedarach-Mediated Copper Oxide Nanoparticles Against Multidrug-Resistant Klebsiella pneumoniae.","authors":"Nimra Arshad, Mohsin Tassawar Cheema, Aatif Amin, Rida Fatima, Mounir M Bekhit, Nady K Elbarbary, Muhammad Shafiq, Mohsin Khurshid","doi":"10.1007/s00284-025-04540-3","DOIUrl":"https://doi.org/10.1007/s00284-025-04540-3","url":null,"abstract":"<p><p>The increasing prevalence of multidrug-resistant pathogens such as Klebsiella pneumoniae, which causes pneumonia and acute respiratory distress syndrome (ARDS) with high mortality rates, poses a significant clinical challenge. Fourth-generation antibiotics, such as cefepime, are often considered the last line of defense against these pathogens. The emergence of cefepime-resistant K. pneumoniae (CRKP) compromises the efficacy of available antibiotics and highlights the importance of new alternative therapies. This study directed the phyto-synthesis of copper oxide (CuO) nanoparticles using Melia azedarach leaf extract and evaluated their antibacterial efficacy, both individually and synergistically with cefepime, against multidrug-resistant Klebsiella pneumoniae through in vitro and in vivo models. The phyto-synthesized copper oxide nanoparticles were characterized using UV-Vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), field emission scanning electron microscopy (FESEM), and energy-dispersive X-ray analysis (EDAX). The antimicrobial efficacy of phyto-synthesized CuONPs, both alone and in combination with the antibiotic cefepime, was assessed through disk diffusion, minimum inhibitory concentration (MIC) assays, and in vivo experimentation. The phyto-synthesized CuONPs were characterized as crystalline, hexagonal in shape, and less than 30 nm in size. The antimicrobial efficacy was confirmed by inhibition zones ranging from 12 to 14 mm against multidrug-resistant Klebsiella pneumoniae. The CuONPs showed strong antimicrobial activity, with a minimum inhibitory concentration (MIC) of 2.25 µg/mL against the pathogenic strain. In comparison, cefepime alone showed no antimicrobial activity. Notably, when CuONPs were combined with cefepime, a synergistic effect was observed, lowering the MIC to 1.92 µg/mL. This study addresses the in vitro and in vivo analysis of CuONPs, with cefepime exerting a potent synergistic effect against the multidrug-resistant strain Klebsiella pneumoniae. This combined treatment resulted in an 82% inhibition rate, a significant reduction in bacterial burden, improved histopathological profiles, faster wound healing, and a regulated immune response.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 12","pages":"555"},"PeriodicalIF":2.6,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145285877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diversity, Isolation and Bioactive Secondary Metabolites of Endophytic Fungi in Duchesnea indica (Andr.) Focke.","authors":"Guiwen Li, Hui Zhang, Gongnian Xiao, Lina Zhu, Shengli Yang","doi":"10.1007/s00284-025-04523-4","DOIUrl":"https://doi.org/10.1007/s00284-025-04523-4","url":null,"abstract":"<p><p>Endophytic fungi in medicinal plants serve as important microbial resources, known to produce bioactive compounds resembling those of their host plants. Duchesnea indica, a traditional Chinese medicinal herb with centuries of application, demonstrates multiple therapeutic properties including detumescence, detoxification, antimicrobial and anticancer effects. Despite its medicinal value, the diversity and community composition of endophytic fungi in this plant species remain insufficiently characterized. This study comprehensively examined the diversity, biological activities and secondary metabolites of endophytic fungi across different tissues (roots, stems, leaves and fruits) of D. indica collected from Hangzhou during April 2023, employing both culture-dependent and culture-independent methodologies. High-throughput Illumina sequencing of the ITS1 region (culture-independent methodology) established Ascomycota and Basidiomycota as the dominant fungal phyla colonizing D. indica. Root tissues displayed the highest fungal abundance and diversity, whereas stems showed the most pronounced tissue specificity. Through culture-dependent methods, 42 fungal strains were successfully isolated, with genomic DNA extracted using commercial kits and subsequently analyzed through ITS sequencing, revealing classification into 9 genera. Antimicrobial evaluation via Oxford cup assays identified 22 fungal strains exhibiting inhibitory activity against Staphylococcus aureus and 12 strains effective against Escherichia coli. CCK-8 cytotoxicity assays demonstrated significant cytotoxic potential among most isolates, with strain PS4 (Aspergillus sp.) showing particularly strong antimicrobial and cytotoxic properties. GC-MS analysis of PS4's ethyl acetate extract detected 33 bioactive constituents. Notably, Colletotrichum and Cercospora appeared potentially involved in enhancing triterpene content in D. indica, offering novel perspectives for further investigation of endophytic fungi in medicinal plants.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 12","pages":"554"},"PeriodicalIF":2.6,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145285870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic and Pathogenic Characterization of Vibrio alginolyticus CG-1 Isolated from Crassostrea gigas.","authors":"Hyoun Joong Kim, Kyung-Il Park, Sang Guen Kim","doi":"10.1007/s00284-025-04552-z","DOIUrl":"https://doi.org/10.1007/s00284-025-04552-z","url":null,"abstract":"<p><p>The Pacific oyster (Crassostrea gigas) is a species of significant economic importance in global aquaculture. Vibrio alginolyticus, a halophilic marine bacterium widely distributed in coastal environments, is recognized as an opportunistic pathogen in both humans and marine organisms. However, the genomic determinants of V. alginolyticus pathogenicity in oysters remain poorly understood. The strain CG-1 was isolated from diseased Pacific oysters collected during a mortality event in Tongyeong, South Korea. Here, we report the complete genome sequence of strain CG-1, which comprises two circular chromosomes measuring 3,535,696 bp (chromosome I) and 1,806,773 bp (chromosome II), with GC contents of 44.61% (chromosome I) and 44.66% (chromosome II). The genome contains 4,785 protein-coding sequences, 127 tRNA genes, 37 rRNA genes, and one tmRNA. Plasmids were not identified. Functional annotation assigned 96.21% of the coding sequences to clusters of orthologous groups (COG) categories primarily related to metabolism, transcription, and inorganic ion transport. The genome encodes multiple virulence-associated genes including components of the type III secretion system, extracellular enzymes, motility and chemotaxis regulators, outer membrane proteins, iron acquisition systems, and antibiotic resistance genes. A pathogenicity assay revealed that V. alginolyticus CG-1 induced dose-dependent mortality in Pacific oyster larvae, with an LD₅₀ of 5.04 × 10⁶ CFU/mL. The clinical symptoms were comparable to those caused by major pathogens such as V. coralliilyticus, although the overall mortality rates were comparatively lower. Overall, this study provides valuable insights into the genome-based mechanisms and pathogenicity of V. alginolyticus in oyster larvae, underscoring its role as an emerging marine pathogen with the potential to impact aquaculture health and sustainability.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 12","pages":"556"},"PeriodicalIF":2.6,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145285904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic Insights into Azole Resistance Mechanisms in Candida tropicalis Among Hematological Malignancy Patients with Candidemia.","authors":"Yalu Ren, Qiongfang Zhu, Yining Wu, Li Ju, Jia Liu, Meili Shen, Weiwei Wu, Jun Qiu, Jie Xu","doi":"10.1007/s00284-025-04535-0","DOIUrl":"https://doi.org/10.1007/s00284-025-04535-0","url":null,"abstract":"<p><p>Invasive candidiasis caused by Candida tropicalis poses a significant threat to patients with hematological malignancies. The increasing prevalence of azole resistance among C. tropicalis isolates complicates treatment strategies, necessitating a detailed understanding of the underlying resistance mechanisms. We conducted a genomic analysis of 15 C. tropicalis isolates collected from patients with hematological malignancies between 2017 and 2021. Whole-genome sequencing (WGS) was performed to obtain high-resolution genomic data. Three strains of C. tropicalis showed azole sensitivity in vitro susceptibility testing and the remaining 12 strains were azole resistant. MLST clade 4 emerged as the predominant azole-resistant clone. All azole-resistant isolates carried the ERG11 mutations A395T and C461T, and most carried the UPC2 mutation T503C. A consistent copy number variation (CNV) at the ERG11 locus was observed in all resistant isolates, suggesting its pivotal role in azole resistance. Our integrated analysis, including SNP, dN/dS ratio, and CNV, confirmed ERG11 as a central player in C. tropicalis' resistance mechanisms in the study. This study provides a comprehensive genomic analysis of azole-resistant C. tropicalis isolates from patients with hematological malignancies. The identification of key genomic alterations, particularly those in the ERG11 gene, offers valuable insights for clinical management and underscores the need for continuous resistant gene surveillance to guide antifungal stewardship in oncology settings.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 12","pages":"552"},"PeriodicalIF":2.6,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145279164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}