Current Microbiology最新文献

Photosynthetic Toxicological Effects of Organic Extracts from Zanthoxylum bungeanum Leaves on Controlling the Microcystis aeruginosa Blooms.

IF 2.3 3区生物学

Current Microbiology Pub Date : 2024-12-19 DOI: 10.1007/s00284-024-04026-8

Jie Cheng, Long Tan, Xue Lu, Mengyu Zheng, Chengshuai Xu, Wei Xu, Tong Jiang, Chaobo Zhang

{"title":"Photosynthetic Toxicological Effects of Organic Extracts from Zanthoxylum bungeanum Leaves on Controlling the Microcystis aeruginosa Blooms.","authors":"Jie Cheng, Long Tan, Xue Lu, Mengyu Zheng, Chengshuai Xu, Wei Xu, Tong Jiang, Chaobo Zhang","doi":"10.1007/s00284-024-04026-8","DOIUrl":"https://doi.org/10.1007/s00284-024-04026-8","url":null,"abstract":"In recent years, the frequent occurrence of algal blooms has posed continuous threats to aquatic ecosystems and social safety. Environmentally friendly algae-inhibiting methods utilizing allelopathic substances offer advantages such as convenient application and low costs, presenting a bright application prospect in the fields of water and ecological restoration. This study aimed to investigate the procedure for extracting total flavonoids from Zanthoxylum bungeanum leaves and assess allelopathic mechanism of Z. bungeanum leaf extracts on Microcystis aeruginosa. The optimal extraction conditions were determined as follows: solvent-to-material ratio = 10 mL/g; extraction time = 55 min; ethanol volume fraction = 80%; and ultrasonic temperature = 80 °C. Antioxidant activity tests revealed that Z. bungeanum leaf extracts exhibit superior capacity compared to Butylated hydroxytoluene (BHT) but inferior to Vitamin C (VC). Moreover, high-concentration Z. bungeanum leaf extracts can disrupt the structures of oxygen-evolving complexes at the donor sides of photosystem II (PSII) reaction center and influence energy distribution at PSII reaction centers, thereby inhibiting electron transmission activities at both donor and receptor sides of the PSII reaction center. The elucidated allelopathic mechanism of Z. bungeanum leaf extracts provides theoretical evidence for the algal-bloom control and the development of allelopathic algae-inhibiting agents, offering a reference for the further comprehensive development and utilization of Z. bungeanum.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 1","pages":"48"},"PeriodicalIF":2.3,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142853524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of Tridax procumbens Secondary Metabolites Anti-Tuberculosis Activity by In Vitro and In Silico Methods.

IF 2.3 3区生物学

Current Microbiology Pub Date : 2024-12-19 DOI: 10.1007/s00284-024-04033-9

Jayashri Seetharaman, R Akshaya Priya, Reya Rene Philip, M Muthuraj, D Sankari

{"title":"Evaluation of Tridax procumbens Secondary Metabolites Anti-Tuberculosis Activity by In Vitro and In Silico Methods.","authors":"Jayashri Seetharaman, R Akshaya Priya, Reya Rene Philip, M Muthuraj, D Sankari","doi":"10.1007/s00284-024-04033-9","DOIUrl":"https://doi.org/10.1007/s00284-024-04033-9","url":null,"abstract":"Mycobacterium tuberculosis is a human pathogen that causes Tuberculosis (TB) disease. Researchers have reported the activity of traditional medicinal plants against human pathogens. However, antimycobacterial studies of medicinal plants against M. tuberculosis remain limited. Thus, the purpose of this study is to characterize the phytochemical profile, antibacterial and antimycobacterial activity of Tridax procumbens towards H37Rv. The antibacterial activity was elucidated by the inhibitory zone formed around the disc by performing disk diffusion method. Tridax antimycobacterial activity measured by Microplate Alamar Blue Assay (MABA) infers the sample is sensitive to H37Rv at MIC (minimum inhibitory concentration) 600 µg/mL. BACTEC MGIT 960 DST identifies the sample is susceptible to H37Rv and Rifampicin resistant (RR). Antiproliferative, functional group determination and mechanism of action of secondary metabolites were performed by MTT, Fourier transform infrared spectroscopy (FTIR) and Gas Chromatography-mass spectrometry (GC-MS). The phytocompounds antimycobacterial efficacy is further supported by molecular docking data. The binding interactions of ligands with gyrA gene revealed (S,Z)-Heptadeca-1,9-dien-4,6-diyn-3-ol molecule as a prominent phytocompound with a binding affinity of -6.6 kcal/mol.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 1","pages":"50"},"PeriodicalIF":2.3,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142863476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deciphering the Antimicrobial and Antibiofilm Efficiency of Thyme Essential Oil Encapsulated Zeolitic Imidazole Framework-8 Against Foodborne Pathogens.

IF 2.3 3区生物学

Current Microbiology Pub Date : 2024-12-19 DOI: 10.1007/s00284-024-04025-9

Suganathan Muthalagu, Suganthy Natarajan

{"title":"Deciphering the Antimicrobial and Antibiofilm Efficiency of Thyme Essential Oil Encapsulated Zeolitic Imidazole Framework-8 Against Foodborne Pathogens.","authors":"Suganathan Muthalagu, Suganthy Natarajan","doi":"10.1007/s00284-024-04025-9","DOIUrl":"https://doi.org/10.1007/s00284-024-04025-9","url":null,"abstract":"Food Packaging using antibacterial substances plays a vital role in food industry in controlling contamination caused by food borne pathogens. Stability and pH dependent degradation characteristics of zeolitic imidazole framework-8 (ZIF-8) makes its suitable candidate for biomedical applications. The present study focuses on thyme essential oil (TEO) encapsulated in ZIF-8 to achieve a synergistic antibacterial effect and prolonged drug release, aiming to extend the shelf life of food products. Optical, structural and surface characterizations of TEO encapsulated within ZIF-8 (ZIF-8@TEO) reveals successful incorporation of TEO into ZIF-8 nanoparticles. High drug loading and pH dependent release rate with higher release rate at acidic condition (75%) makes ZIF-8 a promising drug delivery system. ZIF-8@TEO exhibited potent antimicrobial against Staphylococcus aureus, Bacillus subtilius and Pseudomonas aeruginosa, also attenuated the biofilm forming ability of these food borne pathogens. In vitro and in vivo toxicity studies reveal the non-hemolytic and non-toxic nature indicating its biocompatible nature. The results of the study reveal that ZIF-8@TEO nanoconjugate can be used for the development of novel antibacterial nanocomposite-based films for food packaging applications.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 1","pages":"49"},"PeriodicalIF":2.3,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142863454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation, Structure, and Biological Activities of Exopolysaccharides from Actinomycetes: A Review.

IF 2.3 3区生物学

Current Microbiology Pub Date : 2024-12-18 DOI: 10.1007/s00284-024-03999-w

Xiao-Lian Wei, Song-Yi Mo, Rola Ali-Saeed, Hong Zeng

引用次数: 0

Occurrence, Multidrug Resistance, SCCmec Typing of Methicillin-Resistant Staphylococcus aureus from Farmed Eggs and Environment. 养殖蛋类和环境中耐甲氧西林金黄色葡萄球菌的发生率、耐多药性和 SCCmec 分型。

IF 2.3 3区生物学

Current Microbiology Pub Date : 2024-12-18 DOI: 10.1007/s00284-024-04020-0

Shumaila Taskeen, Randhir Singh, Jasbir Singh Bedi, Anil Kumar Arora, Rabinder Singh Aulakh, Jaswinder Singh

{"title":"Occurrence, Multidrug Resistance, SCCmec Typing of Methicillin-Resistant Staphylococcus aureus from Farmed Eggs and Environment.","authors":"Shumaila Taskeen, Randhir Singh, Jasbir Singh Bedi, Anil Kumar Arora, Rabinder Singh Aulakh, Jaswinder Singh","doi":"10.1007/s00284-024-04020-0","DOIUrl":"https://doi.org/10.1007/s00284-024-04020-0","url":null,"abstract":"A comparative cross-sectional study was conducted on farmed eggs (n: 480) and environmental samples (n: 72; feed, water, and poultry droppings) from twenty-four deep litter and caged poultry layer farms across Punjab, India. The study noted a significantly higher occurrence of Staphylococcus aureus (31.67%; 95% CI 26.37-36.96%) and methicillin-resistant S. aureus (MRSA) (32.5%, 95% CI 23-43.3%) in deep litter farms (P < 0.05). The logistic regression analysis revealed while the odds of presumptive MRSA from egg isolates were high (Odds ratio: 2.714; 95% CI 1.172, 6.284) in deep litter, conversely, resistance against ceftriaxone (0.279; 95% CI 0.129, 0.605), tetracycline (0.227; 95% CI 0.104, 0.497), and erythromycin (0.46; 95% CI 0.218, 0.973) was low. The multi-drug resistance was high and nearly equal; 68.53%, and 72.22% in deep litter and cage systems respectively. Additionally, around 89.65% and 77.7% of MRSA from deep litter and cage systems had multiple antibiotic resistance index > 0.2 respectively. Among the resistance genes under study, the odds of mecA and tetL were 2.417 and 8.029 times higher in deep litter. The hierarchical clustering of MRSA isolates based on the antibiogram profile depicted the formation of a large number of cluster solutions with a mix of isolates from both systems. The SCCmec typing revealed a predominance of type V in both the systems (34.4%; deep litter and 33.3%; cage), with a scarce distribution of other types and subtypes viz., deep litter: types III; 13.7%, IVc; 6.8%, II; 6.8%, IVa, and IVb; 3.4% and cage system: types III and IVc; 11.1%. To, the best of our knowledge current study is the first-ever comprehensive study on comparative occurrence, AMR profile, molecular characterization, and typing of MRSA from farmed eggs and environment from India.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 1","pages":"47"},"PeriodicalIF":2.3,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142845868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of Multiple Copies of the Lon Protease Gene Resulted in Increased Antibiotic Production, Osmotic and UV Stress Resistance in Streptomyces coelicolor A3(2). 多拷贝 Lon 蛋白酶基因的表达提高了链霉菌 A3(2) 的抗生素产量、抗渗透压和抗紫外线能力。

IF 2.3 3区生物学

Current Microbiology Pub Date : 2024-12-17 DOI: 10.1007/s00284-024-04021-z

Halil Yilmaz, Emine Yaradir, Sedef Tunca

{"title":"Expression of Multiple Copies of the Lon Protease Gene Resulted in Increased Antibiotic Production, Osmotic and UV Stress Resistance in Streptomyces coelicolor A3(2).","authors":"Halil Yilmaz, Emine Yaradir, Sedef Tunca","doi":"10.1007/s00284-024-04021-z","DOIUrl":"https://doi.org/10.1007/s00284-024-04021-z","url":null,"abstract":"The genus Streptomyces is a group of gram-positive bacteria that exhibit a distinctive growth pattern characterised by elongated, branched hyphae. Streptomyces coelicolor A3(2), which produces at least five different antibiotics, is a model organism that is widely used in genetic studies. There are very few studies in Streptomyces on the ATP-dependent Lon protease, which has very important functions in every organism and is particularly responsible for protein homeostasis. The aim of this study was to construct and characterize a recombinant S. coelicolor strain expressing the lon gene on a multicopy plasmid. For this purpose, the lon gene was first cloned in Escherichia coli under the control of the glycerol-inducible promoter of pSPG, and its expression in S. coelicolor A3(2) cells was demonstrated by RT-qPCR. In contrast with the initial hypothesis, increased lon expression did not affect cell growth seriously. Instead, it increased the cell's tolerance to osmotic and UV stress and led to a significant increase in antibiotic production. The recombinant strain produced 27 times more actinorhodin and 43 times more undecylprodigiosin than the wild-type strain after 120 h of fermentation. To our knowledge, this is the first study to demonstrate the effects of expression of the lon gene on a high copy number plasmid in Streptomyces.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 1","pages":"43"},"PeriodicalIF":2.3,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142845791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CRISPR/Cas9-Engineering for Increased Amylolytic Potential of Microbes for Sustainable Wastewater Treatment: A Review.

IF 2.3 3区生物学

Current Microbiology Pub Date : 2024-12-17 DOI: 10.1007/s00284-024-04024-w

Yatika Dixit, Preeti Yadav, Hitakshi Asnani, Arun Kumar Sharma

{"title":"CRISPR/Cas9-Engineering for Increased Amylolytic Potential of Microbes for Sustainable Wastewater Treatment: A Review.","authors":"Yatika Dixit, Preeti Yadav, Hitakshi Asnani, Arun Kumar Sharma","doi":"10.1007/s00284-024-04024-w","DOIUrl":"10.1007/s00284-024-04024-w","url":null,"abstract":"Amylases are pivotal enzymes with extensive industrial applications, including food processing, textile manufacturing, pharmaceuticals, and biofuel production. Traditional methods for enhancing amylase production in microbial strains often lack precision and efficiency. The advent of CRISPR/Cas9 technology has revolutionized genetic engineering, offering precise and targeted modifications to microbial genomes. This review explores the potential of CRISPR/Cas9 for improving amylase production, highlighting its advantages over conventional methods. This review discusses the mechanism of CRISPR/Cas9, the identification and targeting of key genes involved in amylase synthesis and regulation, and the optimization of expression systems. Additionally, current review examines case studies demonstrating successful CRISPR/Cas9 applications in various microbial hosts. The review also delves into the integration of CRISPR/Cas9 in wastewater treatment, where genetically engineered amylolytic strains enhance the degradation of complex organic pollutants. Despite the promising prospects, challenges such as off-target effects and regulatory considerations remain. This review provides a comprehensive overview of the current advancements, challenges, and future directions in the application of CRISPR/Cas9 technology for amylase production and environmental biotechnology.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 1","pages":"44"},"PeriodicalIF":2.3,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142845848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microfluidic Chip-based Enrichment and Nucleic Acid Extraction for Quantitative Detection of Mycobacterium Smegmatis in Aerosols.

IF 2.3 3区生物学

Current Microbiology Pub Date : 2024-12-17 DOI: 10.1007/s00284-024-04027-7

Jie Cheng, Minhan Nie, Yiwei An, Zuanguang Chen, Yanli Tong

{"title":"Microfluidic Chip-based Enrichment and Nucleic Acid Extraction for Quantitative Detection of Mycobacterium Smegmatis in Aerosols.","authors":"Jie Cheng, Minhan Nie, Yiwei An, Zuanguang Chen, Yanli Tong","doi":"10.1007/s00284-024-04027-7","DOIUrl":"https://doi.org/10.1007/s00284-024-04027-7","url":null,"abstract":"Tuberculosis (TB) is ranked as the third most prevalent infectious disease globally. Early detection and treatment are crucial for effective management. Conventional diagnostic methods primarily rely on sputum samples, which present challenges in accessibility and have limited accuracy in certain populations such as children, individuals with HIV, and those with extrapulmonary TB. To address the need for point-of-care diagnostics, this study introduces a rapid diagnostic approach for TB using exhaled breath aerosol as a more easily obtainable specimen. Mycobacterium smegmatis, a non-pathogenic bacterium genetically similar to Mycobacterium tuberculosis, is used as a surrogate organism. The method involves the use of microfluidic chips for concentrating and electrolyzing mycobacteria in the aerosol, followed by extracting and quantifying nucleic acids using real-time fluorescence quantitative PCR. Notably, successful enrichment and quantification of bacterial content were achieved even at a minimal bacterial aerosol concentration of 104 CFU/mL. The developed chips are characterized by their cost-effectiveness, ease of use, high bacterial enrichment, efficient nucleic acid extraction, and low detection threshold (4.4 × 10-18 mol/L). This innovative approach offers a promising method for early TB screening and opens avenues for the rapid identification of other aerosol-transmitted diseases.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 1","pages":"42"},"PeriodicalIF":2.3,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142834610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Endophytic Bacteria Enterobacter cloacae PN7 Promotes Biosynthesis and Accumulation of Saponins in Panax notoginseng.

IF 2.3 3区生物学

Current Microbiology Pub Date : 2024-12-17 DOI: 10.1007/s00284-024-04017-9

Xianjing Lin, Mulan Wang, Feiyan Xie, Yuxi Cheng, Li Yang, Jian Gao, Weiqi Li, Xudong Zhang, Ting Tang

{"title":"Endophytic Bacteria Enterobacter cloacae PN7 Promotes Biosynthesis and Accumulation of Saponins in Panax notoginseng.","authors":"Xianjing Lin, Mulan Wang, Feiyan Xie, Yuxi Cheng, Li Yang, Jian Gao, Weiqi Li, Xudong Zhang, Ting Tang","doi":"10.1007/s00284-024-04017-9","DOIUrl":"https://doi.org/10.1007/s00284-024-04017-9","url":null,"abstract":"Panax notoginseng is an important Chinese medicinal plant. Saponins are the major bioactive secondary metabolites with a wide range of medicinal and commercial value in P. notoginseng, so it is crucial to develop environmentally friendly methods to increase their production. The symbiotic relationship between endophytic bacteria and host plants offers a sustainable approach to enhance secondary metabolite biosynthesis. In this study, it was reported that the co-cultivation of an endophytic bacterium Enterobacter cloacae PN7, isolated from P. notoginseng and its host plant could greatly promote saponin accumulation in the root of seedlings. After six days of PN7 treatment, the total saponin concentration reached 21.64 mg/g, representing a 2.01-fold increase over the control. Transcriptome sequencing revealed that PN7 induction upregulated key genes in the saponin biosynthetic pathway (including DXS, HMGR, PMK, DS, CYP450, and GTs), modulated 253 plant hormone signaling genes (such as those related to JA, ETH, and ABA), and affected 284 transcription factor genes and 47 ABC transporter genes. Co-expression network analysis identified DEGs related to plant hormone signaling, transcription factors, and ABC transporters in saponin biosynthesis and distribution. The results suggested that JA signaling, mediated by transcription factors, such as bHLH and MYBs, and its interaction with ETH, played crucial roles in saponin biosynthesis. Additionally, potential ABC transporter candidates involved in saponin transport were identified. This study highlights the role of endophytic bacteria in enhancing saponin production in P. notoginseng and opens avenues for further research on microbial-plant interactions in secondary metabolite production.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 1","pages":"41"},"PeriodicalIF":2.3,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142834608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and Characterization of a Novel Cytorhabdovirus Associated with Pear (P. communis 'Bartlett') Through RNA-seq. 通过RNA-seq鉴定梨（P. communis 'Bartlett'）相关新型细胞病毒并确定其特征。

IF 2.3 3区生物学

Current Microbiology Pub Date : 2024-12-16 DOI: 10.1007/s00284-024-04010-2

Malyaj R Prajapati, Nitika Gupta, Pooja Thapa, Damini Diksha, Susheel Kumar Sharma, Virendra Kumar Baranwal

引用次数: 0