Current Microbiology最新文献

{"title":"Microbiome Assembly of Phyllody-Infected Sesame Leaves.","authors":"Sakthivel Krishnan, Charishma Krishnappa, Neelam Sheoran, Bhaskar Reddy, Aundy Kumar","doi":"10.1007/s00284-025-04383-y","DOIUrl":"https://doi.org/10.1007/s00284-025-04383-y","url":null,"abstract":"<p><p>Sesame phyllody disease poses a serious threat to sesame cultivation, often leading to complete crop failure due to the absence of resistant varieties and lack of effective chemical treatments. To explore sustainable alternatives, this study investigated the foliar microbiome by comparing microbial communities in healthy and phyllody-infected sesame plants from severely affected fields. Through combined culture-based techniques and DNA sequencing, we identified 3108 bacterial OTUs spanning several key genera, including Pantoea, Pseudomonas, Allorhizobium, and Xanthomonas. Phytoplasma, the known causal agent, was detected exclusively in symptomatic plants. Co-occurrence network analysis revealed distinct microbial associations: healthy plants showed balanced interactions with Pantoea, Pseudomonas, and Sphingomonas as central hubs, whereas infected plants exhibited a denser, more complex network, indicating phytoplasma-driven shifts in microbial structure. From culturable isolates, 72 bacterial strains were recovered, with Pantoea being predominant in both plant types. Functional predictions highlighted microbial association with nutrient metabolism and biosynthesis of alpha-linolenic acid, a naturally abundant compound in sesame. This first study of the sesame foliar microbiome provides valuable insights into plant-microbiome-phytoplasma interactions and lays a foundation for developing microbiome-based, eco-friendly strategies to manage sesame phyllody disease in the future.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 9","pages":"399"},"PeriodicalIF":2.3,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144674112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Presence of Human-Associated Clade Marker Gene HAC-G19 in Streptococcus suis Isolated From Humans and Swine.","authors":"Rujirat Hatrongjit, Kulsatri Sittichottumrong, Nattakan Meekhanon, Ratchadaporn Ungcharoen, Zou Geng, Parichart Boueroy, Peechanika Chopjitt, Marcelo Gottschalk, Jinquan Li, Anusak Kerdsin","doi":"10.1007/s00284-025-04387-8","DOIUrl":"https://doi.org/10.1007/s00284-025-04387-8","url":null,"abstract":"<p><p>Streptococcus suis is a zoonotic pathogen contributing to human and pig infections. Effective techniques to distinguish pathogenic from non-pathogenic pathotypes of S. suis is required for public health surveillance. Herein, we developed and evaluated a PCR approach detecting the human-associated clade (HAC)-G19 marker gene to identify the pathogenic pathotype of S. suis. The study was conducted on 599 human strains, 82 diseased-pig strains, and 299 clinically healthy pig strains. PCR analysis revealed that 98.8% and 75.6% were HAC-G19 positive in human and diseased-pig strains, whereas 67.6% of healthy pig strains were negative for the HAC-G19 gene. Statistical analysis revealed that the HAC-G19 gene was more significantly present in diseased-associated isolates than in healthy pig isolates (p-value < 0.001), being with OR of 50.4 (95% CI = 32.1-79.5). Diagnostic accuracy was 87.4% (95% CI = 85.1-89.49%) with sensitivity and specificity were 96.1% (95% CI = 94.3-97.4%) and 67.6% (95% CI = 61.9-72.8%), respectively. This demonstrated that PCR of HAC-G19 has a potential to predict pathogenic pathotype for S. suis.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 9","pages":"400"},"PeriodicalIF":2.3,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144674113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Streptomyces gilvus sp. nov., Actinomycete Isolated from Cassava (Manihot esculenta Crantz) Rhizosphere Soil.","authors":"Janpen Tangjitjaroenkun, Chanwit Suriyachadkun, Wipaporn Ngaemthao, Orawan Chunhachart, Rungnapa Tangchitcharoenkhul","doi":"10.1007/s00284-025-04388-7","DOIUrl":"https://doi.org/10.1007/s00284-025-04388-7","url":null,"abstract":"<p><p>A novel actinomycete designated CME 23^T was isolated from cassava rhizosphere soil collected from Chon Buri province, Thailand. The strain was characterized using a polyphasic approach and classified as a member of the genus Streptomyces. Strain CME 23^T formed spiral chains of non-motile cylindrical spores with a smooth surface developed on aerial mycelia. The cell wall peptidoglycan contained LL-diaminopimelic acid. Rhamnose, ribose, mannose, and glucose were found in its whole-cell hydrolysates. The predominant menaquinones were MK-9(H₈) and MK-9(H₆). The predominant fatty acids were iso-C_15:0, anteiso-C_15:0, and iso-C_16:0. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside, and three unidentified phospholipids. The 16S rRNA gene sequence analysis indicated that strain CME 23^T shared the highest sequence similarity with Streptomyces curacoi DSM 40107^T (98.8%). The average amino acid identity (ANI) values and digital DNA-DNA hybridization (dDDH) values between the genome sequence of strain CME 23^T and closest type strains of Streptomyces curacoi DSM 40107^T, Streptomyces cylindrosporus 7R015^T, and Streptomyces shaanxiensis CCNWHQ 0031^T, were 83.0-88.5% and 28.0-32.8%, respectively. The G + C content of the genome DNA was 71.1%. Based on comparative analyses of genotypic and phenotypic data, the actinomycete strain CME 23^T (= TBRC 15810^T = NBRC 115859^T) represents a novel Streptomyces species, for which the name Streptomyces gilvus sp. nov. is proposed. The in vitro assay showed that strain CME 23^T produced IAA, siderophore, as well as solubilize phosphate and zinc.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 9","pages":"398"},"PeriodicalIF":2.3,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144674114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological and Transcriptomic Response Mediated by Heat Stress in Pleurotus Pulmonarius.","authors":"Zhenghui Lu, Binrong Ke, Hongyi Lin, Bin Yuan, Lina Ke, Meiyuan Chen, Yuanping Lu","doi":"10.1007/s00284-025-04367-y","DOIUrl":"https://doi.org/10.1007/s00284-025-04367-y","url":null,"abstract":"<p><p>Heat stress, one of the major abiotic stresses, affected the growth, fruit body formation and survival of edible mushrooms. Morphological analysis of the Pleurotus pulmonarius Jinxiu displayed that heat stress significantly triggered the inhibited growth of mycelium, weak antifungal activity, yellow water exudation and reduced yield. In order to investigate the underlying mechanism regulating the heat stress response of P. pulmonarius, we analyzed transcriptomic alterations resulting in response to heat stress using high-throughput RNA sequencing of P. pulmonarius strain Jinxiu under heat stress and normal conditions. Totally, we obtained 1,955 deferentially expressed genes. We observed that P. pulmonarius regulated the expression of genes involved in protein processing in endoplasmic reticulum, glycerophospholipid metabolism and glycerolipid metabolism, glutathione metabolism, energy metabolism and carbohydrate metabolism, and transcription factor to response to heat stress. We found that heat shock protein genes could contribute to defense against heat damage and fungal infection. The down-expression of steroid biosynthesis genes in mycelium treated with heat stress might result in weaken antifungal activity and fruit body formation with consequent of fungal infection and reduced yield. This study provided a valuable database for identifying candidate genes and pathways in response to heat stress. Furthermore, it laid the foundation for molecular breeding of new P. pulmonarius strains with improvement of heat-tolerant characters and yield.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 9","pages":"397"},"PeriodicalIF":2.3,"publicationDate":"2025-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144667395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decomposition of Nelumbo nucifera Litters with Three Initial Biomasses Significantly Affected the Microbial Community and Assembly in Sediment.","authors":"Fan Wu, Deyou Feng, Yanan Li, Xin Liu, Xiaohong Zhou, Sisi Xiao, Qingjie Xie","doi":"10.1007/s00284-025-04338-3","DOIUrl":"https://doi.org/10.1007/s00284-025-04338-3","url":null,"abstract":"<p><p>The decomposition of aquatic macrophytes plays a critical role in releasing nutrients and driving biogeochemical cycles in freshwater ecosystems, processes largely mediated by microbial communities. However, the dynamics, assembly mechanisms, and ecological functions of microbial communities, particularly nitrogen-cycling microorganisms, in response to litter decomposition remain poorly understood. This study investigated the impact of Nelumbo nucifera litter decomposition on bacterial and nitrogen-cycling microbial communities, including ammonia-oxidizing bacteria (AOB), ammonia-oxidizing archaea (AOA), and nirS-type denitrifying bacteria, in freshwater sediments using high-throughput sequencing. Decomposition experiments were conducted 240 days using three initial litter biomasses (15, 45, and 75 g). Results revealed that higher initial biomass and extended decomposition times increased mass loss and nutrient release, altering the physicochemical properties of both sediment and overlying water, such as pH, DO, and nutrient concentrations. These changes significantly altered microbial community dynamics, influenced assembly mechanisms and potential ecological functions. Dominant phyla, including Acidobacteriota, Bacteroidota, Chloroflexi, Myxococcota, and Firmicutes, exhibited higher relative abundances in litter-decomposed groups compared to the CK group. Stochastic processes dominated the assembly of AOB and AOA communities, while deterministic processes were more influential for nirS-type denitrifying bacteria. Functional predictions using KEGG pathways highlighted distinct microbial functional profiles associated with nitrogen cycling and carbon metabolism in response to litter decomposition. These findings emphasize the importance of considering both initial biomass and decomposition time when evaluating the effects of plant litter on microbial communities and their functional roles in nutrient cycling, providing valuable insights into the ecological impacts of macrophyte decomposition in freshwater ecosystems.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 9","pages":"396"},"PeriodicalIF":2.3,"publicationDate":"2025-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144667338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic Interactions of Oxidized Amylose Silver Nanoparticles and Topically Applicable Antibiotics Against Staphylococcus aureus.","authors":"Mehmet Erkan Çelebi, Umut Yılmaz, Sema Bagdat, Gülhan Vardar Unlu","doi":"10.1007/s00284-025-04380-1","DOIUrl":"https://doi.org/10.1007/s00284-025-04380-1","url":null,"abstract":"<p><p>Staphylococcus aureus causes serious infections that are increasingly difficult to treat due to antibiotic resistance. In this study, we synthesized oxidized amylose silver nanoparticles (OAAgNPs) and evaluated their synergistic potential with topical antibiotics against multidrug-resistant (MDR) S. aureus. OAAgNPs were synthesized and characterized using spectroscopic and microscopic techniques, confirming their nanoscale structure (5-10 nm) and chemical composition. Antibacterial testing against 50 clinical MDR isolates showed that OAAgNPs alone inhibited bacterial growth. More importantly, combinations with gentamicin (GEN), ciprofloxacin, mupirocin (MUP), or fusidic acid (FA) significantly enhanced antibacterial efficacy. Synergy was strongest with GEN and consistently observed with MUP and FA-antibiotics for which such nanoparticle synergy has rarely been documented. Cytotoxicity assays on mammalian cells demonstrated low toxicity at therapeutically relevant concentrations. Overall, this study highlights the potential of OAAgNP-antibiotic combinations as a promising strategy for managing MDR skin infections. Further research toward clinical formulation development is warranted.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 9","pages":"395"},"PeriodicalIF":2.3,"publicationDate":"2025-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144667397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient Microbial Production of Selenium Nanoparticles by Selenium-Tolerant Alcaligenes sp. HZ-9-3-3.","authors":"Omer Hassan Ali Hassan, Ya-Ru Wei, Zai-Ping Feng, Jia-Mei Pei, Jia-Nan Xu, Wen-Tao Du, Qiang-Qiang Gou, Wen-Di Wang, Xue Zhao, Qin-Cui Shi, Xin-Guo Zhang","doi":"10.1007/s00284-025-04389-6","DOIUrl":"https://doi.org/10.1007/s00284-025-04389-6","url":null,"abstract":"<p><p>Selenium (Se) is a crucial trace element for human and animal health, contributing significantly in various physiological processes. However, its uneven global distribution leads to widespread deficiencies. Nano-selenium (selenium nanoparticles, SeNPs), with low toxicity and high bioavailability, emerges as a promising alternative, and microbial synthesis is the preferred method for its production. In this study, we collected samples from the selenium-rich region of Enshi, Hubei Province, China, and used sodium selenite (Na_₂SeO_₃) gradient screening to isolate microorganisms capable of tolerating high selenium concentrations and efficiently producing SeNPs. We identified nine selenium-tolerant strains that withstand over 0.115 M of sodium selenite. Among these, strain HZ-9-3-3, identified as Alcaligenes sp., tolerated up to 0.231 M and effectively converted selenite to elemental selenium. Optimization using single-factor experiments and central composite design (CCD) established optimal conditions: inoculum size of 12.7% (±0.1%), pH 7, temperature 28 °C, and sodium selenite concentration of 0.0289 M, achieving a conversion rate of 69.7% (±0.1%). The produced SeNPs exhibited moderate antioxidant activity, with IC₅₀ values of 1.5 mg/mL for DPPH radicals and 1.7 mg/mL for hydroxyl radicals, and a reducing power equivalent to 0.842 mg/mL of vitamin C. These findings highlight the potential of strain HZ-9-3-3 for efficient bioconversion of selenium and production of SeNPs with significant antioxidant properties.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 9","pages":"394"},"PeriodicalIF":2.3,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144667394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization and Partial Characterization of Trypsin-Like Protease Enzyme (TLP) from Indonesian Indigenous Lactiplantibacillus plantarum M4 for Digestive Enzyme.","authors":"Armalinda Pertiwi, Trismilah Siswodarsono, Witono Basuki, Wibowo Mangunwardoyo, Molina Indah Pradiva, Sri Rezeki Wulandari, Naufal Hafizh","doi":"10.1007/s00284-025-04301-2","DOIUrl":"https://doi.org/10.1007/s00284-025-04301-2","url":null,"abstract":"<p><p>Trypsin is a digestive enzyme crucial for protein breakdown and the activation of other digestive enzymes. Several studies have shown that trypsin deficiency often leads to various health problems, indicating the need to produce its supplements. These supplements can be obtained from various sources, including different strains of Lactiplantibacillus plantarum, which produces a protease similar to trypsin, called trypsin-like protease (TLP). Therefore, this study aims to enhance TLP production by selecting the optimal medium and fermentation conditions to maximize enzyme activity using Response Surface Methodology (RSM) as an analytical tool, followed by partial enzyme characterization. Lactiplantibacillus plantarum M4 used in this study was obtained from fermented Menteng fruit from Banjarmasin, Indonesia. The optimization results using central composite design in RSM showed that the ideal production medium composition was 1.96% glucose, 0.39% yeast extract, 1.97% skim milk, and pH 6.62, with TLP activity of 0.281 ± 0.012 mU/mL. Ultrafiltration 5000 MWCO was used to concentrate the crude enzyme under ideal conditions, leading to an increase in purity of 11,075 times and an activity of 2,467 mU/mL. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of TLP indicated molecular weights of 29 and 19 kDa as well as stability between 30 and 40 °C, with pH 7-8 indicating optimum activity. Protease activity similar to trypsin was affected by the addition of metal ions, such as Zn²⁺, Ca²⁺, and Ethylenediaminetetraacetic Acid (EDTA). Based on these results, TLP production can be increased by adjusting fermentation conditions to support people with deficiency and preserve health.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 9","pages":"391"},"PeriodicalIF":2.3,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening of Antibiotic-Sensitive Lactic Acid Bacteria (LAB) Associated with the Gut of Penaeus Vannamei and its Application in Shrimp Farming.","authors":"Yiwen Gu, Xiu Lin, Yanxu Lu, Shuguang Fang, Kun Hu, Xin Ju, Liangzhi Li, Zhi Chen","doi":"10.1007/s00284-025-04396-7","DOIUrl":"https://doi.org/10.1007/s00284-025-04396-7","url":null,"abstract":"<p><p>The increasing use of antibiotics in shrimp aquaculture has raised concerns regarding ecological safety and the emergence of antibiotic resistance. This study aimed to isolate and characterize probiotic lactic acid bacteria (LAB) strains from the gut of Penaeus vannamei to evaluate their potential as antibiotic alternatives in aquaculture. Two strains, S1 and S2, were identified with 99.79 and 99.51% similarity to Pediococcus acidilactici DSM 20284 and Enterococcus lactis BT159, respectively. Based on minimum inhibitory concentration (MIC) assays, both strains exhibited high sensitivity to the tested antibiotics. They also demonstrated notable acid and bile salt tolerance, with S1 maintaining a survival rate of 30% at pH 5 and S2 achieving 40% survival at pH 6. Under 0.03% bile salt concentration, survival rates were approximately 60% (S1) and 45% (S2), while S1 retained 15% viability even at 0.9% bile salt. Enzymatic activity analysis indicated that both strains exhibited lower amylase activity compared to other hydrolytic enzymes, with S2 showing higher cellulase and protease activities than S1. Antimicrobial assays revealed that both strains exerted broad-spectrum inhibition against four indicator pathogens. S1 exhibited the strongest inhibitory effect against Aeromonas hydrophila (inhibition zone: 1.6-2.2 cm), whereas S2 showed the highest inhibition against Vibrio parahaemolyticus (1.5-2.4 cm). Feeding trials demonstrated that the combined application of S1 and S2 significantly enhanced shrimp body weight (6.05 ± 0.09 g vs. 9.23 ± 0.2 g, P < 0.01) and survival rate (62.49% ± 1.56 vs. 93.78% ± 2.55, P < 0.01). These findings highlight the potential of Lactobacillus strains as probiotics in shrimp farming, offering a sustainable approach to reducing antibiotic dependency and improving shrimp health and productivity.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 9","pages":"393"},"PeriodicalIF":2.3,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144667396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic Potential of Salvianolic Acid B Produced by Streptomyces sp. M4 Against Multi-drug-Resistant Human Pathogens.","authors":"Manish Sharma, Rajesh Kumari Manhas","doi":"10.1007/s00284-025-04374-z","DOIUrl":"https://doi.org/10.1007/s00284-025-04374-z","url":null,"abstract":"<p><p>Multidrug-resistant bacteria (MDR bacteria), known as superbugs, has become a global issue responsible for lethal infections and high mortality rate. In the search for novel antimicrobial drugs, natural resources have been considered as the potent source of bioactive compounds to control infectious diseases. Microorganisms, especially actinobacteria have shown a positive impact on human health and can produce potent antimicrobial compounds with various bioactivities. In the present investigation, the antibacterial and cytotoxic properties of ethyl acetate (EtOAc) extract and salvianolic acid B from Streptomyces sp. M4 were evaluated. The EtOAc extract and salvianolic acid B displayed antibacterial activity against Bacillus subtilis, Klebsiella pneumoniae sub sp. pneumoniae, methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant Enterococci (VRE), Escherichia coli S1LF and Enterobacter aerogenes with inhibition zones in the range of 14-20 mm and minimum inhibitory concentrations (MIC values) in the range of 7.80-62.25 μg/mL. The DNA nicking assay revealed that salvianolic acid B as well as EtOAc extract provided protection to pBR 322 super coiled DNA from negative effects of free radicals produced by Fenton's reagent. In vitro cytotoxicity assay demonstrated significant cytotoxic activity, with 72.62% and 68.42% inhibition against MG63 osteosarcoma cell line by the salvianolic acid B and EtOAc extract, respectively, at concentration of 5 µM. Salvianolic acid B also exhibited potent activity against drug-resistant bacteria, particularly MRSA and VRE, which are resistant to the standard antibiotics methicillin and vancomycin, respectively. To our best knowledge, this is the first report on antibacterial potential of salvianolic acid B produced by Streptomyces sp. M4 against VRE.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 9","pages":"392"},"PeriodicalIF":2.3,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}