Current Microbiology最新文献

{"title":"Enhancing Growth and Modulation of Gut Microbiota in Red Hybrid Tilapia (Oreochromis sp.) with Host-Associated Leuconostoc lactis as Feed Additive.","authors":"S Y Hew, P Y Tan, K Zhao, H Y Tan","doi":"10.1007/s00284-025-04137-w","DOIUrl":"https://doi.org/10.1007/s00284-025-04137-w","url":null,"abstract":"<p><p>Economic sustainability and the emergence of infectious diseases remain critical challenges in tilapia aquaculture. Lactic acid bacteria (LAB) are well-known feed additives with probiotic effects in improving overall performance of livestock. This study evaluated the effects of host-associated probiotic, Leuconostoc lactis TARicum AI2, on growth performance, antioxidant, immune responses, and gut microbiota of red hybrid tilapia (Oreochromis sp.) by conducting an 86-day feeding trial. The probiotic, previously characterised for its beneficial traits, was coated on commercial feed at a concentration of 10⁹ CFU g^-1. Results showed tilapia fed with probiotic had significant improvement in feed conversion ratio (FCR) and protein efficiency ratio (PER) compared to the control group. Upregulation of cytokine expressions was observed in the probiotic group. Gut microbiota analysis revealed an increased abundance of the genera Clostridium and Leuconostoc in the probiotic-fed tilapia. Beta-diversity indices proved exclusive groups of bacteria present in specific diet only. In conclusion, L. lactis strain TARicum AI2 is a potential probiotic candidate in promoting growth in tilapia as the results suggested it could improve FCR and PER. Feed with lower FCR and higher PER could help tilapia farmers in reducing feed cost, which accounted for the major portion of their production cost. This further enables them to achieve cost-effective, enhanced productivity and economic sustainability in a long run.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 4","pages":"175"},"PeriodicalIF":2.3,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143572524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and Characterization of a Polyvalent Polyclonal Antibody as a Common Capture Antibody for the Detection of Enterotoxigenic Escherichia coli in a Sandwich ELISA.","authors":"Zeus Saldaña-Ahuactzi, José H Gutiérrez-Flores, Victor M Luna-Pineda, Karen Cortés-Sarabia, Fabiola Avelino-Flores, Abdú Orduña-Díaz","doi":"10.1007/s00284-025-04154-9","DOIUrl":"https://doi.org/10.1007/s00284-025-04154-9","url":null,"abstract":"<p><p>Due to its low cost and simplicity, the sandwich enzyme-linked immunosorbent assay (sELISA) is a traditional technique for identifying foodborne pathogens. However, most sELISAs are designed for single foodborne pathogen detection using two specific antibodies, which capture and detect the target bacteria. This study aimed to produce and characterize a common capture polyclonal antibody for Enterotoxigenic Escherichia coli (ETEC), Salmonella Typhimurium, and Shigella flexneri (S. flexneri) by a sELISA. Rabbit polyclonal antibodies (pAbs) were generated against recombinant proteins of CsgA, FhuA, and OmpA, which we called anti-mix. The recombinant proteins generated are conserved in Escherichia coli (E. coli), Salmonella enterica serovar Typhimurium, and S. flexneri species, but not in Listeria monocytogenes (L. monocytogenes) and Enterococcus faecalis (E. faecalis). The anti-mix serum gave a title higher than 1:32,000 by an indirect ELISA using purified recombinant proteins and whole bacteria cultures of the bacteria expressing the antigens but failed to recognize L. monocytogenes and E. faecalis. In addition, a recombinant protein A was purified and used to orient the capture antibodies (anti-mix) in the sELISA. However, no statistically significant difference was found in the assay sensitivity for ETEC detection in spiked milk samples with or without protein A. The assay linearity of sELISA for ETEC detection in Phosphate-buffered saline (PBS) was from 1 × 10⁸ to 1 × 10⁴ cells/mL, and for spiked milk samples was 1 × 10⁸ to 1 × 10⁵ cells/mL. In spiked milk samples, the detection limit of ETEC was lower than PBS, which suggests a negative effect from the matrix analyzed (milk) compared to PBS.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 4","pages":"177"},"PeriodicalIF":2.3,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143572523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing the Power of Bacteriocins: A Comprehensive Review on Sources, Mechanisms, and Applications in Food Preservation and Safety.","authors":"Bharmjeet Singh, Nishant Kumar, Aman Yadav, Rohan, Kriti Bhandari","doi":"10.1007/s00284-025-04155-8","DOIUrl":"https://doi.org/10.1007/s00284-025-04155-8","url":null,"abstract":"<p><p>The Sustainable Development Goals (SDGs) emphasize the importance of food safety, prolonged shelf life, and reduced food waste, all of which rely on effective food preservation methods. Bacteriocins, natural antimicrobial substances produced by lactic acid bacteria (LAB), have potential applications in food preservation. This review highlights the role of LAB-derived bacteriocins in preserving food. Bacteriocins are highly effective against foodborne infections because they target cell membranes, break down enzymes, and interfere with cellular activities. The following study used molecular docking to understand the interaction of bacteriocins and their mode of action. With their natural origin and specific action, bacteriocins offer a promising strategy for preventing foodborne diseases and extending shelf life without impacting sensory characteristics. However, challenges such as stable manufacturing, regulatory hurdles, and cost effectiveness hinder the wide adoption of bacteriocins. Nevertheless, LAB-derived bacteriocins offer a safe and efficient approach to improving food preservation, enhancing food safety, and reducing reliance on artificial preservatives. Moreover, immobilized bacteriocins have the potential to be integrated into antimicrobial packaging films, providing a targeted way to reduce the risk of foodborne pathogen contamination and improve food safety. Exploring novel bacteriocins presents exciting opportunities for advancing food preservation and safety. The present study also highlights recent advancements in food preservation through bacteriocins.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 4","pages":"174"},"PeriodicalIF":2.3,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143572526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and Genetic Profiling of Second-line Drug Resistant Tuberculosis at the Tertiary Care Center of Northern India.","authors":"Nandini Singh, Amresh Kumar Singh, Sushil Kumar, Akanksha Chaudhary, Ashwini Mishra, Narendra Pratap Singh","doi":"10.1007/s00284-025-04152-x","DOIUrl":"https://doi.org/10.1007/s00284-025-04152-x","url":null,"abstract":"<p><p>The emergence of multidrug-resistant tuberculosis (MDR-TB) and extensively drug-resistant TB (XDR-TB) complicates control efforts. We investigated the prevalence and genetic patterns of pre-XDR and XDRTB, by employing second-line line probe assays (SL-LPA). This prospective cohort study was conducted at the Intermediate Reference Laboratory, from November 2023 to May 2024. We screened 1253 samples via Genxpert followed by florescence microscopy, and mutational/resistance analysis via the GenoType MTBDRplus/FL-LPA (first line-line probe assay) and MTBDRsl/SL-LPA for the mutation patterns. We identified 355 rifampicin drug-resistant TB isolates, 260 of which were smear positive samples obtained for FL-LPA and SL-LPA. Finally, 255 MDR/RR-TB patients were analyzed and 73(28.62%) patients exhibited second-line drug (SLD) resistance. Among the cases, 65(89.04%) were found to be resistant solely to fluoroquinolones (FQ), whereas 3(4.11%) were resistant only to aminoglycosides (pre-XDR TB). However, 5(6.8%) patients were resistant to both FQ and aminoglycosides (XDR-TB). We also found that males 44(60.37%) were more affected by SLD resistance than 29(39.72%) females, however SLD resistant females were younger than males (26.97 ± 14.84 vs. 35.84 ± 15.26, p = 0.016). The number of previously treated patients were significantly greater in SLD-resistance than in SLD-sensitive patients. The most common mutation in the gyrA gene was D94G, which was observed in 52(71.23%) cases, followed by A90V in 10(13.69%), D94N and D94A in 4(5.48%), whereas the rrs gene presented the rrsMUT1 (A1401G) mutation in 7(9.58%) and the rrsMUT2(G1484T) mutation in 1(1.37%) of SLD-resistant patients. The alarming rate of SLD-resistance among MDR-TB patients highlights the need for improved management strategies focused on early and accurate diagnosis of DR-TB.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 4","pages":"176"},"PeriodicalIF":2.3,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143572538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tabrizicola caldifontis sp. nov., Isolated from Hot Spring Sediment Sample.","authors":"Neeli Habib, Inam Ullah Khan, Muhammad Saqib, Mohammad Saeid Hejazi, Vahideh Tarhriz, Sohail Ahmad Jan, Cynthia Meza, Aparna Banerjee, Manik Prabhu Narsing Rao, Wen-Jun Li","doi":"10.1007/s00284-025-04156-7","DOIUrl":"https://doi.org/10.1007/s00284-025-04156-7","url":null,"abstract":"<p><p>A Gram-stain-negative, ovoid to rod-shaped, aerobic, non-motile bacterial strain, designated YIM 73028^T, was isolated from a sediment sample collected from a hot spring in Tibet, China. Phylogenetic analysis (based on the 16S rRNA gene sequences) indicated that strain YIM 73028^T belongs to the genus Tabrizicola and showed the highest sequence similarity to the type strain of Tabrizicola aquatica (97.0%). Growth occurred at 30-50 °C (optimum, 37-45 °C) and pH 6.5-8.5 (optimum, pH 7.0-7.5). The respiratory isoprenoid quinone was ubiquinone Q-10. The polar lipids consisted of phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, unidentified amino lipid and unidentified lipid. The major cellular fatty acids (> 10%) were C_18:1 ω7c, C_18:1 ω7c 11-methyl, C_16:0 and C_18:0. The genomic DNA G + C content was 65.7%. The average nucleotide identity value between strain YIM 73028^T and type species of Tabrizicola aquatica was lower than 95-96% threshold recommended for distinguishing novel prokaryotic species. Based on the phenotypic, physiological, chemotaxonomic, genotypic, and phylogenetic data, strain YIM 73028^T represents a novel species of the genus Tabrizicola, for which the name Tabrizicola caldifontis sp. nov. is proposed. The type strain is YIM 73028^T (= KCTC 52713^T = CGMCC 1.16151^T).</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 4","pages":"172"},"PeriodicalIF":2.3,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143572539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enterococcus hailinensis sp. nov., Isolated from Traditional Chinese Pickle.","authors":"Ting-Yu Wang, Hao Wang, Chun Tao Gu","doi":"10.1007/s00284-025-04143-y","DOIUrl":"https://doi.org/10.1007/s00284-025-04143-y","url":null,"abstract":"<p><p>Two enterococcal isolates, designated as strains N48-2^T and N48-9.1, were isolated from traditional Chinese pickle ('Suan cai') and characterized using a polyphasic approach. Phylogenies based on 16S rRNA gene sequences and whole genome sequences indicated that strains N48-2^T and N48-9.1 were most closely related to Enterococcus hermanniensis LMG 12317^T. Strains N48-2^T and N48-9.1 shared the highest 16S rRNA (99.5%), pheS (90.3%), rpoA (97.7% and 97.5%) and concatenated pheS and rpoA (95.1% and 95.0%) gene sequence similarities with the type strain of E. hermanniensis. Strains N48-2^T and N48-9.1 had 86.7%-87.9% ANI, 33.6%-33.8% dDDH and 91.7%-92.2% AAI values with E. hermanniensis DSM 17122^T. Phenotypically, strains N48-2^T and N48-9.1 could be distinguished from DSM 17122^T by acid production from D-galactose, L-sorbose, L-rhamnose and amygdalin, H₂S production, resistance to streptomycin sulfate (50 μg/ml), kanamycin sulfate (50 μg/ml), neomycin sulfate (5 μg/ml) and gentamicin sulfate (5 μg/ml), tolerance to 6% NaCl, and growth at pH 5 and 40 °C. Based on these data, strains N48-2^T (= JCM 37000^T = CCTCC AB 2024127^T = LMG 33662^T) and N48-9.1 represent a novel species, in which the name Enterococcus hailinensis sp. nov., is proposed.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 4","pages":"171"},"PeriodicalIF":2.3,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143566329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic Differences in Antimicrobial Resistance and Virulence Among Key Salmonella Strains of Serogroups B and D1 in Brazilian Poultry.","authors":"Ruy D Chacón, Manuel Ramírez, Dilan Suárez-Agüero, Ana P Arellano Pineda, Claudete S Astolfi-Ferreira, Antonio J Piantino Ferreira","doi":"10.1007/s00284-025-04147-8","DOIUrl":"https://doi.org/10.1007/s00284-025-04147-8","url":null,"abstract":"<p><p>Salmonella is a significant threat to Brazilian poultry, causing economic losses and public health risks. This study analyzed 15 Salmonella isolates along with 45 retrieved complete genomes, including serovars Gallinarum, Pullorum, Enteritidis, Typhimurium, and Heidelberg. Biochemical characterization, antimicrobial susceptibility testing, whole-genome sequencing, and comparative genomics were performed. The studied strains exhibited high levels of antimicrobial resistance, particularly to tilmicosin, penicillin/novobiocin, nalidixic acid, and streptomycin. Genomic analysis revealed diverse virulence factors and antibiotic resistance genes (ARGs), with zoonotic strains showing higher virulence compared to avian-adapted strains. Multiple plasmid types carrying ARGs were identified, highlighting the potential for horizontal gene transfer. Pangenomic and phylogenomic analyses differentiated Salmonella strains from serogroup D1 from those from serogroup B. These findings emphasize the need for comprehensive surveillance and control measures to mitigate the impact of Salmonella on both animal and human health in Brazil.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 4","pages":"173"},"PeriodicalIF":2.3,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143572525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Co-occurrence of mcr-9 and bla_NDM-5 in Multidrug-Resistant Enterobacter hormaechei Strain Isolated from a Patient with Bloodstream Infection.","authors":"Ru Li, Junfeng Wang, Weiting Zhang, Chenhao Zhao, Mingxiao Han, Hong Du, Haifang Zhang","doi":"10.1007/s00284-025-04160-x","DOIUrl":"https://doi.org/10.1007/s00284-025-04160-x","url":null,"abstract":"<p><p>Enterobacter hormaechei (E. hormaechei), a member of the Enterobacter cloacae complex, has emerged as an important pathogen in healthcare-associated infections. In this study, a multidrug-resistant E. hormaechei EH001 co-carrying mcr-9 and bla_NDM-5 was isolated from a patient with bloodstream infection in China. To investigate the genomic characteristics of a multidrug-resistant E. hormaechei EH001 co-carrying mcr-9 and bla_NDM-5, whole-genome sequencing was employed and conjugation experiment was performed by using the recipient E. coli EC600. The antimicrobial susceptibility profiles of E. hormaechei EH001 revealed resistance to the most commonly used antimicrobial agents, with the exception of polymyxin B, polymyxin E, and minocycline. E. hormaechei EH001 was classified as sequence type 78 (ST78) and harbored multiple resistance genes, especially co-carried mcr-9 and bla_NDM-5 located on an IncHI2 plasmid (pMCR9_EH001) and an IncX3 plasmid (pNDM5_EH001), respectively. Both plasmids were successfully co-transferred to E. coli EC600 by conjugation. Analysis of the genetic environment of mcr-9 and bla_NDM-5 revealed the multiple mobile genetic elements, such as insertion sequences (IS) and transposon (Tn). ∆Tn3-IS3000-∆ISAba125-5'-IS5-∆ISAba125-3' were situated upstream of bla_NDM-5, while ble_MBL-trpF-dsbD-CutA-IS26 were situated downstream of it. In addition, rcnR, rcnA, pcoE, pcoS, and IS5 family transposase (IS903B) were situated upstream of mcr-9, while IS3000 was situated downstream of it, followed by bla_CTX-M-3 and IS26. The majority of the plasmid-mediated resistance genes reside within or adjacent to diverse mobile genetic elements, which may potentially promote the horizontal transfer of antibiotic resistance determinants. This study represents the initial investigation providing the detailed genomic characteristics associated with co-occurrence of mcr-9 and bla_NDM-5 in E. hormaechei. The findings advocate for heightened clinical vigilance toward such strains, particularly to prevent nosocomial transmission.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 4","pages":"169"},"PeriodicalIF":2.3,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143566326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antifungal Action of Valencene and Nootkatone Compounds in Association with Fluconazole and Their Mechanism of Action Against Candida spp. and Pichia kudriavzevii Strains.","authors":"Joara Nályda Pereira Carneiro, Antonia Thassya Lucas Dos Santos, Victor Juno Alencar Fonseca, Maria Audilene de Freitas, Francildo Dos Santos Silva, Larissa Alves Lopes de Souza, Nadine Monteiro Salgueiro Araújo, Daniele de Oliveira Bezerra de Sousa, Rafael Guimarães Gomes Silva, João Xavier da Silva Neto, Irwin Rose Alencar de Menezes, Henrique Douglas Melo Coutinho, Maria Flaviana Bezerra Morais-Braga","doi":"10.1007/s00284-025-04133-0","DOIUrl":"https://doi.org/10.1007/s00284-025-04133-0","url":null,"abstract":"<p><p>Candidemia is a public health challenge as it causes thousands of annual deaths and combating it has become difficult due to the development of resistance in Candida spp. Compounds derived from natural products may counter this resistance. Therefore, we evaluate the intrinsic and combined antifungal activity of valencene and nootkatone compounds and their possible mechanism of action against Candida spp. Using the microdilution method, the antifungal effect of sesquiterpenes and their combination with fluconazole was determined. The results comprised the yeast growth curve and its 50% Inhibitory Concentration (IC₅₀). They showed that the compounds alone inhibited microbial growth at a concentration of 1024 µg/mL for valencene being able to kill the fungus Pichia kudriavzevii (Candida krusei), for nootkatone the inhibition occurred at 512 µg/mL and was able to kill the species P. kudriavzevii and Candida tropicalis. Combined with the antifungal, the inhibition occurred at low concentrations (2 and 4 µg/mL) against all strains except P. kudriavzevii, which the combination with nootkatone inhibited at 512 µg/mL. The IC₅₀ revealed inhibition of the strains at higher concentrations in the compounds and fluconazole alone compared to the combination concentrations. In addition, both compounds acted through the production of reactive oxygen species, helping the antifungal against C. albicans and P. kudriavzevii, contributing minimally to compromising membrane viability. Thus, the compounds show promise for combined activity with fluconazole.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 4","pages":"168"},"PeriodicalIF":2.3,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143556065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In-Depth Genome-Based Analysis of Shigella spp. and Escherichia spp.: Resolving Ambiguities and Unveiling Phylogenetic Relationships.","authors":"Guendouz Dif, Nadjette Djemouai, Noureddine Bouras, Abdelghani Zitouni","doi":"10.1007/s00284-025-04158-5","DOIUrl":"https://doi.org/10.1007/s00284-025-04158-5","url":null,"abstract":"<p><p>Although traditionally classified as distinct genera, recent genomic analyses suggest that Shigella species may represent pathogenic clones of Escherichia coli. In this study, we investigated the genetic relationships between Shigella and Escherichia species through comprehensive phylogenomic and taxonomic analyses. Genomic datasets for all validly named species within both genera were retrieved from GenBank. Multiple methods, including 16S rRNA gene sequence analysis, digital DNA-DNA hybridization (dDDH), average nucleotide identity (ANI), Genome BLAST Distance Phylogeny (GBDP), and percentage of conserved proteins (POCP), were employed. These results reveal a high genetic similarity between Shigella species and E. coli, with ANI values exceeding 96% and dDDH values above 70%, indicating that Shigella species fall within the same species as E. coli. Phylogenomic trees, generated from whole-genome sequences and core genes, further corroborated the close evolutionary relationship between these taxa. Furthermore, these analyses challenge the reclassification of Atlantibacter hermannii and Pseudescherichia vulneris, supporting their retention within the genus Escherichia. Based on these findings, we propose the reclassification of Shigella species as subspecies within E. coli and recommend revisiting the taxonomic status of other related species.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 4","pages":"170"},"PeriodicalIF":2.3,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143566331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}