Antifungal Activity and Mechanism of the Novel Antimicrobial Peptide Jelleine-Ic Against Candida albicans.

IF 2.6 3区生物学 Q3 MICROBIOLOGY

Current Microbiology Pub Date : 2025-10-14 DOI:10.1007/s00284-025-04551-0

Lifang Wang, Ke Li, Jie Zhu, Kang Ding, Xiaomin Tao, Xiaomei Xu, Yan Zhu

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引用次数: 0

Abstract

Candida albicans, an opportunistic fungus, is the most common cause of invasive candidiasis globally. Drug resistance and limited number of antifungal compounds highlight the necessity of new antifungal drugs to treat C. albicans infections. Antimicrobial peptides (AMPs) exhibit potential as antifungal agents. The novel AMP Jelleine-Ic designed by our group demonstrated superior antibacterial activity than its parent peptide Jelleine-I. This work investigated the antifungal activity, antibiofilm activity and mechanism of action of Jelleine-Ic against C. albicans. Jelleine-Ic showed stronger antifungal activity against C. albicans than Jelleine-I. It effectively inhibited biofilm formation and destructed the preformed biofilm of C. albicans. Notably, it exhibited synergistic effect when combined with Amphotericin B. At concentrations below 125 μg/mL, it was not hemolytic on rabbit red blood cells. Jelleine-Ic compromised cell wall integrity, increased the permeability of C. albicans cell membrane. Furthermore, upon entering the cell, Jelleine-Ic bound with DNA, altered the expression of genes involved in DNA replication and repair, and induced the production of intracellular reactive-oxygen species. Jelleine-Ic increased the survival of Galleria mellonella infected with C. albicans. Collectively, Jelleine-Ic effectively controlled C. albicans, and could be a potential replacement for antibiotics and other chemical fungicides in the treatment of fungal infections.

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新型抗菌肽Jelleine-Ic对白色念珠菌的抑菌活性及机制研究。

白色念珠菌是一种机会性真菌，是全球侵袭性念珠菌病的最常见原因。耐药性和有限数量的抗真菌化合物突出了新的抗真菌药物治疗白色念珠菌感染的必要性。抗菌肽（AMPs）显示出作为抗真菌剂的潜力。本课组设计的新型AMP jelline - ic抗菌活性优于其亲本肽jelline - i。本文研究了Jelleine-Ic对白色念珠菌的抗真菌活性、抗菌膜活性及其作用机制。jelline - i对白色念珠菌的抑菌活性强于jelline - i。它能有效地抑制白色念珠菌生物膜的形成，破坏预先形成的生物膜。在浓度低于125 μg/mL时，对兔红细胞无溶血作用。jeline - ic破坏细胞壁完整性，增加白色念珠菌细胞膜的通透性。此外，在进入细胞后，jelline - ic与DNA结合，改变了参与DNA复制和修复的基因的表达，并诱导细胞内活性氧的产生。jelline - ic提高了白色念珠菌感染的mellonella的存活率。总的来说，jelline - ic有效地控制了白色念珠菌，并可能成为抗生素和其他化学杀菌剂治疗真菌感染的潜在替代品。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current Microbiology 生物-微生物学

CiteScore

4.80

自引率

3.80%

发文量

380

审稿时长

2.5 months

期刊介绍： Current Microbiology is a well-established journal that publishes articles in all aspects of microbial cells and the interactions between the microorganisms, their hosts and the environment. Current Microbiology publishes original research articles, short communications, reviews and letters to the editor, spanning the following areas: physiology, biochemistry, genetics, genomics, biotechnology, ecology, evolution, morphology, taxonomy, diagnostic methods, medical and clinical microbiology and immunology as applied to microorganisms.