Current Microbiology最新文献

{"title":"Consotaella aegiceratis sp. nov., a Novel Endophytic Bacterium Isolated from Root of Aegiceras corniculatum.","authors":"Yifen Luo, Chengshao Zan, Mingsheng Chen, Fang Liu, Xiaohui Chen, Zhouqing Zheng, Xiao Ma, Li Tuo","doi":"10.1007/s00284-025-04294-y","DOIUrl":"https://doi.org/10.1007/s00284-025-04294-y","url":null,"abstract":"<p><p>A Gram-stain-negative, aerobic, short rod-shaped and motile bacterium, designated strain CSK11QG-6^T was isolated from root of Aegiceras corniculatum taken from Shankou Mangrove Ecological National Nature Reserve in Guangxi Zhuang Autonomous Region, China. Global alignment based on 16S rRNA gene sequences indicated that strain CSK11QG-6^T shared the highest identity of 96.5% with Consotaella salsifontis USBA 369^T. Phylogenetic analyses based on 16S rRNA gene sequences and genome sequences indicated that strain CSK11QG-6^T clustered together with Consotaella salsifontis USBA 369^T. Strain CSK11QG-6^T had a genome size of 5.42 Mbp, with a N50 of 97.8 kb and coverage of 138 × . The DNA G + C content was 64.8%. The orthologous average nucleotide identity and digital DNA-DNA hybridization values of strain CSK11QG-6^T with related species fell below the proposed species delineation threshold of 95-96% and 70%, respectively, indicating that strain CSK11QG-6^T represented a novel species. Strain CSK11QG-6^T contained C_18:1ω7c and C_16:0 as the predominant fatty acids, and uniquinone-10 as the respiratory quinone. The major polar lipids were phosphatidylglycerol, phosphatidylcholine, diphosphatidylglycerol, phosphatidylethanolamine, an unidentified aminolipids and two unidentified phospholipids. Strain CSK11QG-6^T exhibited chemical characteristics of fatty acids, lipids and quinones typical for the genus Consotaella, but could be clearly differentiated from Consotaella by many of phenotypic characteristics. Based on phylogenomic and phylogenetic analyses coupled with phenotypic and chemotaxonomic characterizations, strain CSK11QG-6^T represents a novel species of the genus Consotaella, for which the name Consotaella aegiceratis sp. nov. is proposed. The type strain is CSK11QG-6^T (= MCCC 1K07176^T = JCM 35044^T).</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 7","pages":"309"},"PeriodicalIF":2.3,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144149681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EPS-Producing Bacteria Promote Aggregation in Soil Preventing the Leaching Loss of Nutrient.","authors":"Kapudeep Karmakar, Dipankar Roy, Srija Pal, Bratati Chowdhury, Ashok Choudhury","doi":"10.1007/s00284-025-04295-x","DOIUrl":"https://doi.org/10.1007/s00284-025-04295-x","url":null,"abstract":"<p><p>Leaching loss of nutrients from the agricultural field is a major concern in areas receiving excessive rainfall. The water-soluble nutrients are therefore lost due to leaching and runoff water. This leads to excessive dependence on chemical fertilizers. In this regard, the role of indigenous exopolysaccharide (EPS) producing bacteria in stabilizing the nutrient-holding capacity is far greater than conceived. The sub-Himalayan terai region, located at Cwa zone (monsoon type with dry winter, Köppen's classification) in India, which receives heavy rainfall (> 3000 mm), is a suitable location to study the role of such bacteria. A culture-dependent analysis of EPS-producing bacteria showed their comparatively lower abundance throughout the year. The EPS-associated bacterial microenvironment on vermiculite particles under aerobic and anaerobic conditions was visualized using a Confocal Laser Scanning Microscope (CLSM). The cells of EPS producers were found distributed within the EPS matrix, pushing each other away, showing a higher organic matter secretion (EPS) per unit of the bacterial cells. Since EPS has adhesive properties, the indigenous EPS-producing bacteria were amended to the soil, and the formation of macroaggregates was analyzed. Two in-house EPS-producing bacteria were used to monitor if they can reduce the leaching loss of nutrients. In vitro, assays on nutrient-holding capacity by soil-column-flow-through revealed that these bacteria-treated soil retained more nutrients. Till now, there were no reports on the role of the genus Serratia in improving the nutrient-holding capacity. For the first time, we have shown that using such soil-dwelling genera can improve the formation of stable aggregates and prevent nutrient loss.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 7","pages":"307"},"PeriodicalIF":2.3,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144141630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring Escherichia coli EGY Type II L-Asparaginase Variant of Unique Glutaminase Activity: Cloning, Expression, Biochemical Characterization, and Molecular Docking Analysis.","authors":"Rawan Alshamy, Nehal Aly, Nefertiti El-Nikhely, Hisham A Nematalla, Mohamed Elkewedi, Hesham Saeed","doi":"10.1007/s00284-025-04233-x","DOIUrl":"https://doi.org/10.1007/s00284-025-04233-x","url":null,"abstract":"<p><p>Microbial L-asparaginase II is a cornerstone biopharmaceutical drug for the effective treatment of leukemia and other blood cancers. However, the associated cellular resistance mechanisms in response to the treatment require effective management and necessitate searching and developing of better versions of the enzyme. Overall, the current study described a new E. coli type II L-ASNase variant of unique L-glutaminase co-activity that was cloned into pET28a ( +) vector and expressed in E. coli BL21(DE3) pLysS as a cytosolic protein with a molecular weight of 38.390 kDa. The recombinant E. coli L-ASNase was purified by affinity chromatography and showed high specific activity of 7179.5 U/mg. The purified enzyme exhibited enormous thermal stability and its affinity for L-asparagine (L-Asn) and L-glutamine (L-Gln) was in silico analyzed by molecular docking and experimentally verified which indicated relatively similar affinity. The purified E. coli L-ASNase demonstrated high dual activities toward L-Asn (Km and Vmax 0.627 mM and 385 µmol min^-1) with 90% specificity toward L-Gln (Km and Vmax 0.715 mM and 376 µmol min^-1). The molecular docking study suggested that E. coli EGY L-ASNase follows a single displacement mechanism of catalysis based on the interacting residues analysis from a computational insight. Biochemical characterization and immunogenicity prediction were carried out to contribute for better understanding of the immunogenic determinants along with the other features of the new variant to consider its potentiality for in vitro and in vivo cytotoxicity testing in future studies.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 7","pages":"306"},"PeriodicalIF":2.3,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144141569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Naringenin as a Potent Natural Biofilm Inhibitor of Pseudomonas aeruginosa in Diabetic Foot Ulcers Through lasR Competitive Inhibition.","authors":"Anjali Warrier, Kapaettu Satyamoorthy, Thokur Sreepathy Murali","doi":"10.1007/s00284-025-04283-1","DOIUrl":"https://doi.org/10.1007/s00284-025-04283-1","url":null,"abstract":"<p><p>Chronic non-healing foot ulcers are a major complication in diabetic patients, contributing to significant morbidity and mortality. Microorganisms in these wounds form biofilms, conferring greater virulence and enhanced protection from antibiotics. Hence, we examined naringenin, and other natural compounds like chlorofuranone, 4-nitropyridine N-oxide, and quercetin as a positive control against the major pathogenic organism that forms biofilm in foot ulcers. Here, we focused on Pseudomonas aeruginosa, which produces high levels of biofilm in diabetic foot ulcers. Naringenin (47.10 µg/ml for PA21; 124.7 µg/ml for PA333) and other natural compounds were tested for their ability to inhibit biofilm formation and virulence in vitro, and their effect on biofilm-associated gene expression was studied. The biofilm inhibitory mechanism of naringenin was elucidated using in silico analysis and in vitro reporter gene assay. In vitro biofilm assays, confocal and scanning electron microscopy showed that natural compounds effectively inhibited biofilm, without causing cell death. Treatment with these compounds significantly altered the expression of genes associated with quorum sensing in P. aeruginosa, such as lasR, pslA, algA, gacS, and pelA. Naringenin decreased the production of major virulence factors in P. aeruginosa. Molecular docking showed that naringenin exhibited the strongest binding affinity to LasR, and the same was validated by reporter gene assay using plasmid pSB1142 indicating its role as a competitive inhibitor in the las quorum sensing system in P. aeruginosa. The findings of this study could be extrapolated to in vivo diabetic wound infection models to help optimize the use of naringenin in effective biofilm control for better wound management in diabetic patients.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 7","pages":"305"},"PeriodicalIF":2.3,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144141698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Characterization of Escherichia coli Causing Community-Acquired Urinary Tract Infections in a Suburban Area in West Bengal, India: Dispersal of Multidrug-Resistant Epidemic ST131 Clone Carrying CTX-M-1.","authors":"Urmy Biswas, Abhi Mallick, Taru Singh, Subhadip Das, Surojit Das","doi":"10.1007/s00284-025-04271-5","DOIUrl":"https://doi.org/10.1007/s00284-025-04271-5","url":null,"abstract":"<p><p>Antimicrobial resistance (AMR) imperils global public health overlooking the epidemiological situation of community-acquired infections, especially in low- and middle-income countries. We conducted this study in a suburban community in West Bengal, India, to comprehend the AMR and bacterial forensics of Escherichia coli causing community-acquired urinary tract infections (CAUTIs). Antibiograms, β-lactamases, plasmid-mediated quinolone resistance genes, and plasmids were investigated to explore AMR. Bacterial forensics was assessed using phylogrouping, genetic fingerprinting, and strain typing. Antibiograms revealed that > 75% of isolates had a multiple-antibiotic resistance (MAR) index of > 0.2. Fosfomycin remained 100% effective as an empiric drug, followed by nitrofurantoin (> 75%), aminoglycosides (> 65%), and co-trimoxazole (> 50%). Multidrug-resistant (MDR) was frequent (> 75%), with < 5% being extensively drug-resistant, including two colistin-resistant isolates. Piperacillin/tazobactam showed promising activity against the ESBL-producing isolates (> 70%) mostly carrying CTX-M-15 and TEM-1 (> 50%), which declined in the presence of OXA-1 (> 20%). TEM-253 and 254, two novel alleles, were assigned. Two of the thirteen NDM-5-producing carbapenem-resistant isolates co-harbored OXA-48. Among fluoroquinolone-resistant isolates (> 80%), aac(6')-lb-cr (> 20%) was the most common, followed by qnrS (> 10%). Plasmid of > 212 kb was prevalent (> 60%), especially in MDR isolates. IncF was the sole plasmid in > 85% of isolates, with > 90% having a MAR index > 0.2. Phylogroup B2 was prevalent (> 35%), primarily associated with the ESBL-producing ST131 clone (> 80%). Carbapenem-resistant isolates belonged to phylogroup A/sequence-type361, C/ST2851, D/ST405, and F/ST648. ESBL-producing isolates were largely clonally related. This maiden molecular study reveals the infiltration of MDR epidemic clones in a suburban community in eastern India, guiding genomic surveillance for strategic management of AMR at the national level.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 7","pages":"303"},"PeriodicalIF":2.3,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144132190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reclassification of Microbacterium chocolatum Takeuchi and Hatano 1998 as a Later Heterotypic Synonym of Microbacterium aurantiacum Takeuchi and Hatano 1998.","authors":"Rattiya Janthanom, Syed Raziuddin Quadri, Ngoc Tung Quach, Manik Prabhu Narsing Rao, Arinthip Thamchaipenet","doi":"10.1007/s00284-025-04276-0","DOIUrl":"https://doi.org/10.1007/s00284-025-04276-0","url":null,"abstract":"<p><p>The taxonomic position of Microbacterium chocolatum was evaluated through 16S rRNA gene sequence and genome-based analyses. The pairwise similarity of the 16S rRNA gene sequence retrieved from the genome data between Microbacterium aurantiacum KACC 20510^T and Microbacterium chocolatum JCM 12412^T was 99.3%, while between Microbacterium kitamiense JCM 10270^T and M. chocolatum JCM 12412^T was 99.4%. The similarity between M. aurantiacum KACC 20510^T and M. kitamiense JCM 10270^T was 100%. In the phylogenomic tree (based on 71 bacterial single-copy genes), M. aurantiacum KACC 20510^T, M. kitamiense JCM 10270^T and M. chocolatum JCM 12412^T showed no genetic divergence. The average nucleotide identity (ANI), and digital DNA-DNA hybridization (dDDH) values among M. aurantiacum KACC 20510^T, M. chocolatum JCM 12412^T and M. kitamiense JCM 10270^T were above the cut-off values (95-96% for ANI and 70% for dDDH) for species delineation. This study supports the previous reclassification of Microbacterium kitamiense Matsuyama et al. 1999 as a later heterotypic synonym of Microbacterium aurantiacum Takeuchi and Hatano 1998, and also proposes that Microbacterium chocolatum Takeuchi and Hatano 1998 is a later heterotypic synonym of Microbacterium aurantiacum Takeuchi and Hatano 1998.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 7","pages":"304"},"PeriodicalIF":2.3,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144132191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Co-Occurrence of Beauvericin and Fumonisin Producing Ability of Fusarium Strains Isolated from Crop Plants in Hungary.","authors":"Ákos Suhajda, Mohammed Al-Nussairawi, Ines Amara, Csilla Sörös, Rita Tömösközi-Farkas, Balázs Kriszt, Milán Farkas, Mátyás Cserháti","doi":"10.1007/s00284-025-04243-9","DOIUrl":"10.1007/s00284-025-04243-9","url":null,"abstract":"<p><p>Beauvericin (BEA) is an emerging mycotoxin with wide-ranging bioactivity (antimicrobial and insecticide), making it a potential target for drug and pesticide development. BEA primarily produced by Beauveria, Isaria, and Fusarium species. The BEA-producing abilities of a collection of 100 Fusarium strains isolated from maize were tested using a gene-specific primer (Beas_1, Beas_2) by PCR. Among all, 23 were found to have the beauvericin synthetase (BEAS) gene sequence, which is responsible for the production of BEA. Fusarium proliferatum (6) and F. verticillioides (14) strains were producing the highest BEA concentrations. The toxin-producing ability of the strains was investigated in small bioreactors. Parallel with BEA, the most frequent Fusarium toxins such as deoxynivalenol (DON), T2, HT-2, zearalenone (ZEA), fumonisin B1 (FB1), and fumonisin B2 (FB2) were also measured. Only FB1 and FB2 were observed above the detection limit, the coexistence of the FBs and BEA was measured in high concentration. In all BEA-producing strains, the FBs production could be detected. The highest BEA concentration was 3131 mg/kg, and the highest FB1 and FB2 concentrations were 4393 mg/kg and 1390 mg/kg, respectively. In the present study, the gene sequences responsible for the production of BEA in F. verticilloides isolates have not only been detected but also demonstrated with UHPLC-ESI-MS/MS to be capable of biosynthesis. From the phylogenic analysis of the BEAS gene sequences, the assumption could be made that the ability to produce BEA was conferred via horizontal gene transfer.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 7","pages":"302"},"PeriodicalIF":2.3,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12101999/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144126800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial Diversity and Organoleptic Properties in Traditionally Fermented Pineapple Beverage Ameliorated with Blueberry or Pomegranate.","authors":"Prattipati Geya Sai Manjusha, Annamaneni Sandhya, Bee Hameeda","doi":"10.1007/s00284-025-04284-0","DOIUrl":"https://doi.org/10.1007/s00284-025-04284-0","url":null,"abstract":"<p><p>The trend of traditionally fermented beverages (TFBs) is gaining importance because of their health benefits and high nutritional value. Post-era of COVID-19 pandemic and increase in consumer awareness on health has taken U-turn for consumption of naturally fermented foods for gut health and immune homeostasis. Consumption of fresh vegetables and fruits is attributed with numerous health benefits. Nevertheless, limited information is available how natural fermentation of fruits can alter the microbial diversity, nutritional value, and distinct flavor. Therefore, we wanted to compare four different samples of TFBs, pineapple without brown sugar (FPC), pineapple amended with brown sugar (FPO), or pineapple amended with brown sugar and blueberry (FPB), or pineapple amended with brown sugar and pomegranate (FPP). The microbial diversity in TFBs after 72 h of fermentation analyzed through Illumina MiSeq (2 × 250) revealed that Firmicutes were predominant over Proteobacteria and Bacteroidetes in bacterial diversity; likewise, Ascomycota was predominant over Basidiomycota in fungal diversity. Most abundant genera of bacteria included Acetobacter, Lactobacillus, Lactococcus, Pediococcus, Gluconobacter, Weissela, Klyuvera, and Enterobacter, and fungi were Naumovozyma, Metschnikowia, Candida, Meyerozyma, and Kazachstania. At the end of fermentation (72 h), physicochemical characteristics of all the four samples revealed pH from 2.91 to 4.64 and Brix degrees lined up below 15 which fall under the category of ready to serve (RTS) beverages. Overall, the findings of this study explain FPB showed better physicochemical and organoleptic properties along with significant diversity of Firmicutes (Lactobacillus and Lactococcus), recommending it as potential fermented beverage.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 7","pages":"301"},"PeriodicalIF":2.3,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144119207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ecological Impacts of Sunflowers on Soil Microbial Communities: Insights from Full-Length 16S rRNA Sequencing.","authors":"Seong-Jun Chun, Yingshun Cui, Jihoon Kim, Jun-Woo Lee, Sung Min Han, Kyong-Hee Nam","doi":"10.1007/s00284-025-04273-3","DOIUrl":"10.1007/s00284-025-04273-3","url":null,"abstract":"<p><p>Sunflower (Helianthus annuus L.), a widely cultivated oilseed crop, is occasionally found growing in non-cultivated areas in South Korea, raising concerns about its unintentional potential ecological impacts. This study investigated the effects of two naturalized sunflower cultivars with varying seed dispersal densities on soil microbial communities in a simulated natural ecosystem. A field experiment was performed using 2 × 2 m plots designed to simulate natural ecosystems. The crops incorporated two types of sunflowers with different seed densities. Microbiome samples were collected at the pre-sowing, growing, and flowering stages and subsequently analyzed using the PacBio Sequel platform. Using long-read sequencing of full-length 16S rRNA genes via the PacBio Sequel II platform, we identified 9,885 amplicon sequence variants (ASVs), including key indicator species significantly correlated with seed density. While sunflowers demonstrated limited dominance and no significant alterations to overall community structure, specific microbial taxa, such as Phycicoccus ginsenosidimutans and Flavisolibacter ginsengiterrae, were strongly associated with sunflower growth. These findings provide critical insights into the potential ecological impacts of sunflower introductions and underscore the importance of full-length 16S rRNA sequencing for accurate microbial identification. This study highlights the need for continuous monitoring and contributes to advancing risk assessment methodologies for naturalized plants in natural ecosystems.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 7","pages":"297"},"PeriodicalIF":2.3,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144110297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic Diversity and Phylogenetic Analysis of Watermelon Mosaic Virus in Türkiye: Insights into Emerging and Classic Populations.","authors":"Ali Ferhan Morca, Sevgi Coşkan, Faruk Öncü, Birol Akbaş, Adyatma Irawan Santosa, Serkan Yeşil, Ali Çelik","doi":"10.1007/s00284-025-04269-z","DOIUrl":"10.1007/s00284-025-04269-z","url":null,"abstract":"<p><p>Watermelon mosaic virus (WMV, genus Potyvirus) is a significant pathogen affecting cucurbit production in the Western Black Sea and Central Anatolia regions of Türkiye, with limited knowledge of its genetic diversity hindering molecular control strategies. In this study, 19 complete coat protein (CP) gene sequences from WMV isolates collected from four cucurbit species were analyzed using RT-PCR, MEGA11 for phylogenetics, and DnaSP v6.12.03 for population genetics. The phylogenetic analysis, including 48 global isolates, classified eight Turkish isolates into the \"emerging\" (EM) group, 11 into the \"classic\" (G1) group, and identified a novel minor phylogroup. While a recombination signal was detected in isolate KP164988, none was observed in the Turkish isolates. Genetic diversity analysis showed that the G1 group was less divergent than the EM group, with all populations under strong purifying selection (ω = 0.022-0.243) and displaying recent expansion, likely due to variations in newly reported isolates. The results emphasize the genetic separation between EM and G1 groups and reveal frequent gene flow between Turkish and global populations. This comprehensive survey provides valuable insights into the genetic structure and diversity of WMV in Türkiye, supporting other reports on minor phylogroups within the virus populations.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 7","pages":"298"},"PeriodicalIF":2.3,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144109444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}