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Jestvirus, a Novel Genus in the Family Autotranscriptaviridae Infecting Aeromonas. 感染气单胞菌的自转录病毒科新属Jestvirus。
IF 2.8 3区 生物学
Current Microbiology Pub Date : 2026-10-21 DOI: 10.1007/s00284-026-05104-9
Kewei Chen, Jiaxiang Li, Jinxuan Chen, Kaiyang Zheng, Liang Zhou, Yue Su, Shujuan Sun, Xin Chen, Wei Wang, Yantao Liang, Hongbin Shao, Yundan Liu, Xiao Zou
{"title":"Jestvirus, a Novel Genus in the Family Autotranscriptaviridae Infecting Aeromonas.","authors":"Kewei Chen, Jiaxiang Li, Jinxuan Chen, Kaiyang Zheng, Liang Zhou, Yue Su, Shujuan Sun, Xin Chen, Wei Wang, Yantao Liang, Hongbin Shao, Yundan Liu, Xiao Zou","doi":"10.1007/s00284-026-05104-9","DOIUrl":"10.1007/s00284-026-05104-9","url":null,"abstract":"<p><p>Aeromonas species are ubiquitous, aquatic, opportunistic pathogens; however, their viral predators remain underexplored. Here, we isolated and characterized Aeromonas phage vB_AsaP_JS1, a dsDNA phage with a short non-contractile tail that infects Aeromonas salmonicida. Comparative analyses indicate that vB_AsaP_JS1 diverges from representative T7-like phages, with gene content similarity below 50% and a maximum amino acid identity of 70% or less. It forms a supported monophyletic clade in both phylogenomic and protein-based trees. Gene-sharing network analysis further shows that vB_AsaP_JS1 and its closest relatives form a distinct, isolated cluster, clearly separated from all currently recognized genera within the family Autotranscriptaviridae and related lineages of Caudoviricetes. Collectively, these features satisfy ICTV criteria for genus demarcation and support the establishment of a novel phage genus, tentatively designated Jestvirus. Functional annotation highlights a compact transcriptional module containing a single-subunit T7-like RNA polymerase and a streamlined replication packaging operon. Several putative host-interaction-related genes, including a deoxycytidine kinase homolog, a PelC-like protein, and a putative glycosidase, are encoded in the genome; their possible roles in nucleotide scavenging or host interaction during infection represent hypotheses that require experimental validation. Metagenomic read mapping reveals sequence-level signals related to vB_AsaP_JS1 in wastewater datasets from multiple geographic regions, suggesting a potentially wider environmental distribution for this phage lineage than previously recognized, though confirmation of phage identity and prevalence will require further investigation. Together, our results expand the known genomic diversity of T7-like phages and provide taxonomic, functional, and ecological evidence for a new phage lineage within Caudoviricetes.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 12","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149237314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Enhanced Lichenysin Production in Bacillus licheniformis by Physicochemical Mutagenesis Combined with High-Throughput Screening. 物化诱变结合高通量筛选提高地衣芽孢杆菌地衣素产量。
IF 2.8 3区 生物学
Current Microbiology Pub Date : 2026-10-21 DOI: 10.1007/s00284-026-05198-1
Zhengjun Pang, Lingzhi Zhang, Zhengrong Tao, Wenshuo Zhang, Bo Zhang, Fenghuan Wang
{"title":"Enhanced Lichenysin Production in Bacillus licheniformis by Physicochemical Mutagenesis Combined with High-Throughput Screening.","authors":"Zhengjun Pang, Lingzhi Zhang, Zhengrong Tao, Wenshuo Zhang, Bo Zhang, Fenghuan Wang","doi":"10.1007/s00284-026-05198-1","DOIUrl":"10.1007/s00284-026-05198-1","url":null,"abstract":"<p><p>Lichenysin is a cyclic lipopeptide secondary metabolite produced by Bacillus licheniformis, with excellent biocompatibility and diverse biological activities. It has broad application potential in biomedicine, agricultural pest control, energy production, and environmental remediation. Nevertheless, the improvement of wild strain with complex genetic backgrounds through genetic and metabolic pathway modifications poses significant challenges in terms of time and cost, which has become a bottleneck limiting the diversified development of lichenysin-producing strains. In this research, ultraviolet (UV) irradiation, N-methyl-N'-nitro-N-nitrosoguanidine (NTG), ethyl methanesulfonate (EMS) and composite mutagenesis strategies were systematically utilized to conduct multiple rounds of mutagenesis using Bacillus licheniformis Ali5 as the parental strain. Subsequently, the mutants were screened through high-throughput screening in a 96-well plate based on the hexadecylpyridinium chloride-fluorescein (CPC-FL) fluorescence detection principle. After two rounds of mutagenesis and genetic stability assessment, five high-yielding mutant strains with stable genetic traits (UV40-4, NT382-5, EMS191-1, NV341-3 and EV52-1) were successfully screened. The results of flask culture illustrated that the mutants were superior to the parental strain in biomass, the titer/OD<sub>600</sub> and lichenysin yield (up to 20.6%). This study established a quick and scalable mutagenesis and high-throughput screening system, which provided high-quality strains for its industrial-scale production.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 12","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149237242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
In Vitro Synergistic Activity of Cefepime/Zidebactam Plus Aztreonam Against Metallo-β-Lactamase-Producing Enterobacterales. 头孢吡肟/齐地巴坦加氨曲南对产金属β-内酰胺酶肠杆菌的体外增效作用。
IF 2.8 3区 生物学
Current Microbiology Pub Date : 2026-10-21 DOI: 10.1007/s00284-026-05185-6
Matteo Boattini, Sara Comini, Alessandra Cornacchia, Francesco Pomilio, Patrice Nordmann, Gabriele Bianco
{"title":"In Vitro Synergistic Activity of Cefepime/Zidebactam Plus Aztreonam Against Metallo-β-Lactamase-Producing Enterobacterales.","authors":"Matteo Boattini, Sara Comini, Alessandra Cornacchia, Francesco Pomilio, Patrice Nordmann, Gabriele Bianco","doi":"10.1007/s00284-026-05185-6","DOIUrl":"10.1007/s00284-026-05185-6","url":null,"abstract":"","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 12","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149237251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Inoculum Effect in Multidrug-Resistant Gram-Negative Isolates: Impact on Ceftazidime and Cefepime Susceptibility. 多重耐药革兰氏阴性分离株的接种效应:对头孢他啶和头孢吡肟敏感性的影响。
IF 2.8 3区 生物学
Current Microbiology Pub Date : 2026-10-21 DOI: 10.1007/s00284-026-05172-x
Shahinda Rezk, Noha Fawzy, Amel Elsheredy, Rasha Emad Mansour, Ola Kader
{"title":"Inoculum Effect in Multidrug-Resistant Gram-Negative Isolates: Impact on Ceftazidime and Cefepime Susceptibility.","authors":"Shahinda Rezk, Noha Fawzy, Amel Elsheredy, Rasha Emad Mansour, Ola Kader","doi":"10.1007/s00284-026-05172-x","DOIUrl":"10.1007/s00284-026-05172-x","url":null,"abstract":"<p><p>Multidrug- or extensively drug-resistant (MDR or XDR) Gram-negative infections remain a global challenge, further complicated by the inoculum effect (IE), which can significantly alter the in vitro activity of β-lactam antibiotics and may contribute to treatment failure in infections with a high bacterial burden. In this study, we evaluated the IE of ceftazidime (CAZ) and cefepime (FEP) against multidrug-resistant Gram-negative clinical isolates. Fifty isolates, including Escherichia coli (E. coli), Klebsiella pneumoniae (K. pneumoniae), Pseudomonas aeruginosa (P. aeruginosa), and Proteus mirabilis (P. mirabilis), were included in the current study. Minimum inhibitory concentrations (MICs) of CAZ and FEP were determined at standard (10<sup>5</sup> CFU/mL) and high (10<sup>7</sup> CFU/mL) inoculum levels. The IE was defined as an increase of ≥8-fold in MIC. Five β-lactamase genes, bla<sub>CTXM-9</sub>, bla<sub>OXA-2</sub>, bla<sub>OXA-10</sub>, bla<sub>tem-1</sub>, and bla<sub>SHV</sub>, were screened by polymerase chain reaction. Tested isolates were mainly MDR (74.0%) or XDR (26.0%). A significant increase in MIC values at high inoculum was observed for both CAZ and FEP in E. coli and K. pneumoniae (p<0.001), with a marked shift in susceptibility. In K. pneumoniae, CAZ exhibited significantly greater stability against IE than FEP (p = 0.041). In contrast, no significant IE was detected in P. aeruginosa or P. mirabilis. The presence of β-lactamase resistance genes is a significant predictor of FEP IE (OR=14.7, p=0.026). Significant positive correlations between MICs at standard and high inoculum, alongside inverse correlations between standard MIC and fold change in E. coli and K. pneumoniae, were observed. The IE significantly compromises the activity of CAZ and FEP in E. coli and K. pneumoniae, with important clinical implications in high bacterial burden infections. However, CAZ may be superior compared to FEP in IE conditions, particularly for K. pneumoniae. The IE is primarily organism-dependent and may be influenced by β-lactamase gene patterns such as bla<sub>oxa</sub> genes with bla<sub>CTXM-9</sub>, bla<sub>tem-1</sub>, or bla<sub>SHV</sub>, particularly for FEP. These findings highlight the limitations of standard susceptibility testing and emphasize the need for careful antibiotic selection in severe, life-threatening Gram-negative infections.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 12","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149237245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genomic Exploration of CRISPR-Cas Systems in Raoultella species: Diversity and Architecture. Raoultella物种CRISPR-Cas系统的基因组探索:多样性和结构。
IF 2.8 3区 生物学
Current Microbiology Pub Date : 2026-10-21 DOI: 10.1007/s00284-026-05157-w
Ahmed Velit Abuden, Çiğdem Yilmaz Çolak
{"title":"Genomic Exploration of CRISPR-Cas Systems in Raoultella species: Diversity and Architecture.","authors":"Ahmed Velit Abuden, Çiğdem Yilmaz Çolak","doi":"10.1007/s00284-026-05157-w","DOIUrl":"10.1007/s00284-026-05157-w","url":null,"abstract":"<p><p>Clustered regularly interspaced short palindromic repeats and CRISPR-associated protein (CRISPR-Cas) systems are adaptive immune mechanisms that protect bacteria against invading mobile genetic elements. They can influence horizontal gene transfer, phage resistance, and antimicrobial resistance (AMR) dynamics. Although these systems have been investigated in several Klebsiella species, information on historically defined Raoultella species remains limited. In this study, 103 complete genomes representing Raoultella species, currently placed within the broader Klebsiella lineage, were analyzed to characterize the distribution, architecture, and diversity of CRISPR-Cas systems. CRISPR-Cas loci were identified using CRISPRCasFinder and CRISPRone, followed by subtype classification, direct repeat and leader sequence analyses, spacer matching, Cas1-based phylogenetic analysis, and AMR determinant screening. Complete CRISPR-Cas systems were detected in 23 genomes, predominantly in R. ornithinolytica strains. All complete systems were chromosomally located, whereas plasmid-associated orphan CRISPR arrays were observed in selected R. ornithinolytica strains. Two major architectures were identified, with Type I-E being dominant and Type I-F restricted to fewer strains. Direct repeat analysis revealed six consensus sequences, while leader regions displayed conserved AT-rich motifs and strain-specific divergence. Spacer analyses suggested historical interactions with phages and plasmids, particularly mobile genetic elements associated with Klebsiella and other Enterobacteriaceae. Cas1 phylogeny showed subtype-dependent clustering, indicating that CRISPR-Cas evolution does not strictly follow core genome phylogeny. The coexistence of predicted CRISPR-Cas loci and AMR determinants indicates that the presence of these loci does not necessarily preclude the carriage of resistance determinants. Overall, this study expands current knowledge of CRISPR-Cas diversity among the analyzed Raoultella genomes and provides a genomic framework for future functional studies.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 12","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149237299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Culturable Endophytic Fungi of Atractylodes macrocephala: Influence of Tissue Type and Geographic Location on Their Antifungal, Therapeutic, and Cytotoxic Potentials. 白术可培养内生真菌:组织类型和地理位置对其抗真菌、治疗和细胞毒性的影响。
IF 2.8 3区 生物学
Current Microbiology Pub Date : 2026-10-21 DOI: 10.1007/s00284-026-05163-y
Yuzhe Li, Yueqing Hong, Kaohua Liu, Yixue Ding, Hao Zheng, Linxin Ye, Siyuan Zhang, Qinger Huang, Jiangnan Shi, Jingyi Xu, Shaokai Xu, Luping Qin, Bo Zhu
{"title":"Culturable Endophytic Fungi of Atractylodes macrocephala: Influence of Tissue Type and Geographic Location on Their Antifungal, Therapeutic, and Cytotoxic Potentials.","authors":"Yuzhe Li, Yueqing Hong, Kaohua Liu, Yixue Ding, Hao Zheng, Linxin Ye, Siyuan Zhang, Qinger Huang, Jiangnan Shi, Jingyi Xu, Shaokai Xu, Luping Qin, Bo Zhu","doi":"10.1007/s00284-026-05163-y","DOIUrl":"10.1007/s00284-026-05163-y","url":null,"abstract":"<p><p>Atractylodes macrocephala Koidz. is a medicinal plant that has been widely used as a tonic agent in different ethno-medical systems in East Asia. This study explores the culturable endophytic fungi of A. macrocephala and potential correlations between these fungi and the active components of this medicinal plant. The antipathogenic activity of these endophytic fungi against three species of pathogenic Fusarium was examined. The cytotoxicity of the endophytic fungi extracts was assessed in five human cancer cell lines. A total of 2338 endophytic fungal isolates were obtained from the root, stem, leaf, and rhizome of A. macrocephala. The endophytic fungal community structure in A. macrocephala was influenced by tissue type and geographical location. Trichothecium roseum (Pers.) Link was found to be closely associated with atractylenolide I, a major active component of A. macrocephala. In total, 40 of the 77 endophytic fungal taxa (51.95%) manifested inhibitory effects on the growth of at least one of the fungal pathogens. In addition, metabolites from most fungal endophytes exhibited cytotoxicity against the cancer cells; in particular, AM450 and AM448 were the most potent (IC₅₀ < 5 μg/mL) against MCF-7, A549, KB, KB-VIN, and MDA-MB-231. These extracts showed potent cytotoxicity against cancer cell lines and warrant further study; toxicity testing on normal cells is needed to assess selectivity and therapeutic potential.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 12","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149237302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Crosstalk Role of Intestinal Barrier Damage in the Gut-Organ Axis and its Potential Therapeutic Implications. 肠屏障损伤在肠-器官轴中的串扰作用及其潜在的治疗意义。
IF 2.8 3区 生物学
Current Microbiology Pub Date : 2026-10-21 DOI: 10.1007/s00284-026-05170-z
Jiaxue Feng, Wenwen Xie, Huifei Lu, Li Tian, Xiaohang Shi, Jianbin Huang, Xianhai Zeng, Yichi Zhou, Meizhen Zhao, Baohui Cheng, Haotao Zeng
{"title":"The Crosstalk Role of Intestinal Barrier Damage in the Gut-Organ Axis and its Potential Therapeutic Implications.","authors":"Jiaxue Feng, Wenwen Xie, Huifei Lu, Li Tian, Xiaohang Shi, Jianbin Huang, Xianhai Zeng, Yichi Zhou, Meizhen Zhao, Baohui Cheng, Haotao Zeng","doi":"10.1007/s00284-026-05170-z","DOIUrl":"10.1007/s00284-026-05170-z","url":null,"abstract":"<p><p>Intestinal barrier damage is a pivotal mediator of gut-organ crosstalk and a key contributor to systemic disease. This review systematically summarizes the structural organization of the intestinal barrier, including the mucus layer, epithelial tight junctions, and the lamina propria immune network, and highlights their coordinated roles in regulating intestinal barrier dysfunction and immune homeostasis. Disruption of barrier integrity facilitates the translocation of microbial products, cytokines, and metabolites, thereby promoting systemic inflammation and the pathogenesis of multi-organ diseases, including liver injury, pancreatitis, and cardiovascular disorders. Emerging evidence underscores the critical roles of gut microbiota, immune signaling pathways, and metabolic factors in barrier regulation. Advances in single-cell and spatial omics, together with artificial intelligence (AI)-driven multi-omics integration, are providing novel insights into the cellular heterogeneity and molecular mechanisms underlying barrier dysfunction. Therapeutic strategies targeting barrier restoration-including microbiota-based interventions and pharmacological approaches-show promising potential but require further clinical validation. This review provides a comprehensive framework for understanding intestinal barrier biology and its translational relevance.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 12","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13687403/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149237276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
In Vitro Activity and Synergism of Amphotericin B and the Synthetic Bioinspired Peptide γ31-45PvD1++ Against Fungal Study Models Candida tropicalis and Saccharomyces cerevisiae. 两性霉素B和合成生物激发肽γ31-45PvD1++对热带假丝酵母和酿酒酵母的体外活性及增效作用研究
IF 2.8 3区 生物学
Current Microbiology Pub Date : 2026-10-21 DOI: 10.1007/s00284-026-05197-2
Thaynã A M Souza, Érica de O Mello, Fernanda G Trindade, Felipe F Moreira, Gabriel B Taveira, Milena B Cherene, Larissa M Resende, Sérgio H Seabra, Maura da Cunha, André de O Carvalho, Valdirene M Gomes
{"title":"In Vitro Activity and Synergism of Amphotericin B and the Synthetic Bioinspired Peptide <sub>γ31-45</sub>PvD<sub>1</sub><sup>++</sup> Against Fungal Study Models Candida tropicalis and Saccharomyces cerevisiae.","authors":"Thaynã A M Souza, Érica de O Mello, Fernanda G Trindade, Felipe F Moreira, Gabriel B Taveira, Milena B Cherene, Larissa M Resende, Sérgio H Seabra, Maura da Cunha, André de O Carvalho, Valdirene M Gomes","doi":"10.1007/s00284-026-05197-2","DOIUrl":"10.1007/s00284-026-05197-2","url":null,"abstract":"<p><p>In recent years, following the COVID-19 pandemic, the emergence of antibiotic-resistant microorganisms has increased. This surge has prompted global research into new biologically active molecules, particularly those focused on combination therapies involving antifungal agents. Synthetic antimicrobial peptides have emerged as promising candidates to address this demand. Therefore, the objective of this study was to assess the synergistic therapeutic effects of the synthetic peptide γ<sub>31-45</sub>PvD<sub>1</sub><sup>++</sup> combined with amphotericin B (AmB) on Candida tropicalis and Saccharomyces cerevisiae. This study aimed to identify the synergistic concentration that could facilitate advancement in the development of novel drugs. Here, we demonstrated that synergistic combinations of synthetic peptides and amphotericin B (AmB) effectively induce cell death in C. tropicalis and S. cerevisiae. The checkerboard test revealed synergistic concentrations of 0.048 µM for AmB, 0.39 µM for the synthetic peptide γ<sub>31-45</sub>PvD<sub>1</sub><sup>++</sup> for C. tropicalis, 0.097 µM for AmB, and 0.78 µM for the synthetic peptide γ<sub>31-45</sub>PvD<sub>1</sub><sup>++</sup> for S. cerevisiae. The mechanism of action underlying the death of these yeasts when exposed to these synergistic combinations involves several processes, including the loss of cell wall integrity, impairment of mitochondrial functionality, DNA condensation, and damage to cell morphology and ultrastructure. The data presented in this study will contribute significantly to the development of new therapies involving combinations of antifungal molecules.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 12","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13687208/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149237248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Lysobacter halodurans sp. nov., a Bacterium Isolated from the Sludge of a Bioreactor Treating Aromatic Compounds. 从处理芳香族化合物的生物反应器污泥中分离的嗜盐溶菌。
IF 2.8 3区 生物学
Current Microbiology Pub Date : 2026-10-21 DOI: 10.1007/s00284-026-05178-5
Chuansheng Geng, Kangni Ren, Jiayu Liu, Hao Wang, Liang Sun, Ping Song, Letian Chen, Tongchu Deng, Meiying Xu
{"title":"Lysobacter halodurans sp. nov., a Bacterium Isolated from the Sludge of a Bioreactor Treating Aromatic Compounds.","authors":"Chuansheng Geng, Kangni Ren, Jiayu Liu, Hao Wang, Liang Sun, Ping Song, Letian Chen, Tongchu Deng, Meiying Xu","doi":"10.1007/s00284-026-05178-5","DOIUrl":"10.1007/s00284-026-05178-5","url":null,"abstract":"<p><p>A novel bacterium, strain e5<sup>T</sup>, was isolated from the sludge of a bioreactor treating aromatic compounds. Cells of strain e5<sup>T</sup> were Gram-stain-negative, rod-shaped, forming yellow and viscous colonies on Reasoner's 2 A agar plates. Phylogenetic analyses based on 16 S rRNA gene showed that strain e5<sup>T</sup> shared 97.3% sequence similarity with Luteimonas arsenica 26-35<sup>T</sup> and formed a distinct lineage in the genus Luteimonas with low bootstrap values. However, phylogenomic analyses indicated that strain e5ᵀ formed a distinct lineage in the genus Lysobacter with high bootstrap values, indicating that strain e5ᵀ is a novel species in this genus. In agreement with the phylogenomic results, the enzyme activities and fatty acid profiles of strain e5ᵀ were consistent with its placement in the genus Lysobacter. Nevertheless, strain e5ᵀ can be distinguished from closely related species by its salt tolerance, phenylacetate assimilation, urease activity, gelatin hydrolysis, and the relative proportions of fatty acids such as iso-C<sub>15:0</sub>, iso-C<sub>16:0</sub>, and iso-C<sub>11:0</sub> 3-OH. These phenotypic and chemotaxonomic data thus provide additional support for the recognition of strain e5ᵀ as a novel species within the genus Lysobacter. Therefore, the name Lysobacter halodurans sp. nov. is proposed. The type strain is e5<sup>T</sup> (= KCTC 92385<sup>T</sup> =GDMCC 1.3249<sup>T</sup>).</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 12","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149237283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Taxonogenomic Investigation of Yoonia aquicola sp. nov., Isolated from Coastal Seawater. 沿海海水中水杨藻的分类基因组学研究。
IF 2.8 3区 生物学
Current Microbiology Pub Date : 2026-10-21 DOI: 10.1007/s00284-026-05174-9
Jaewoo Yoon
{"title":"Taxonogenomic Investigation of Yoonia aquicola sp. nov., Isolated from Coastal Seawater.","authors":"Jaewoo Yoon","doi":"10.1007/s00284-026-05174-9","DOIUrl":"10.1007/s00284-026-05174-9","url":null,"abstract":"<p><p>An alphaproteobacterium, KMU-150<sup>T</sup>, was isolated from a coastal seawater sample collected in the Republic of Korea and investigated using a taxonogenomic approach. Cells were light beige in color, oval- to short rod-shaped, Gram-stain-negative, strictly aerobic, and non-motile. It was able to grow at 0-7.0% NaCl concentrations (w/v), pH 5.5-9.0, and 20-37 °C. A phylogenetic tree based on 16S rRNA gene sequences showed that strain KMU-150ᵀ clustered with the genus Yoonia within the class Alphaproteobacteria and was closely related to Yoonia litorea DPG-5ᵀ, with a sequence similarity of 98.4%. The sole respiratory quinone was Q-10, and the polar lipids consisted of phosphatidylcholine, phosphatidylglycerol, two unidentified aminolipids, and an unidentified phospholipid. C<sub>18:1</sub> ω7c was identified as a major (> 10%) fatty acid. Strain KMU-150<sup>T</sup> had a genome size of 3.4 Mb and a DNA G + C content of 63.0%. Phylogenomic trees robustly placed strain KMU-150ᵀ within the genus Yoonia. The ANI values between strain KMU-150ᵀ and species of the genus Yoonia ranged from 71.6 to 75.1%, and the dDDH value with Y. litorea DPG-5ᵀ was 19.5%, indicating that strain KMU-150ᵀ represents a novel species of the genus. The genome of strain KMU-150ᵀ was predicted to harbor five putative biosynthetic gene clusters, suggesting a possible capacity for secondary metabolite biosynthesis. Moreover, several genes associated with the degradation of biological molecules were identified in the genome of strain KMU-150<sup>T</sup>, indicating substantial potential for the synthesis of enzymes with valuable industrial applications. The taxonogenomic data obtained in this study support the conclusion that strain KMU-150ᵀ represents a novel species of the genus Yoonia, for which the name Yoonia aquicola sp. nov. is proposed. The type strain is KMU-150ᵀ (= KCCM 90340ᵀ = NBRC 117445ᵀ).</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 12","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149237240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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