Current Microbiology最新文献_第2页

Mycobacterium jinjuensis sp. nov., a Novel Acid-Fast Bacterium Isolated from a Pneumonia Patient. 从肺炎患者中分离的一种新型抗酸菌——金菊分枝杆菌。

IF 2.6 3区生物学

Current Microbiology Pub Date : 2025-10-13 DOI: 10.1007/s00284-025-04473-x

Minh Phuong Trinh, Jeong-Ih Shin, Kyu-Min Kim, Thi Oanh Dao, Jeong-Gyu Choi, Myunghwan Jung, Hyung-Lyun Kang, Seung-Chul Baik, Woo-Kon Lee, Jung-Hyun Byun, Min-Kyoung Shin

{"title":"Mycobacterium jinjuensis sp. nov., a Novel Acid-Fast Bacterium Isolated from a Pneumonia Patient.","authors":"Minh Phuong Trinh, Jeong-Ih Shin, Kyu-Min Kim, Thi Oanh Dao, Jeong-Gyu Choi, Myunghwan Jung, Hyung-Lyun Kang, Seung-Chul Baik, Woo-Kon Lee, Jung-Hyun Byun, Min-Kyoung Shin","doi":"10.1007/s00284-025-04473-x","DOIUrl":"https://doi.org/10.1007/s00284-025-04473-x","url":null,"abstract":"A novel Gram-stain-positive, acid-fast, aerobic, slow-growing bacterium, designated strain ND9-15 T was isolated from the sputum of a patient with pneumonia in a tertiary hospital in Jinju, Gyeongsangnam-do, Republic of Korea. The strain exhibited growth at temperatures ranging from 15 to 37 °C, with optimal growth at 25-30 °C. It grew at pH values of 5.0-9.0, with an optimum pH of 7.0-7.5, and in the presence of 0-4% (w/v) NaCl, optimally at 0.5-1%. Based on the 16S rRNA gene sequencing, strain ND9-15 T clustered within the genus Mycobacterium, showing the closest relationship to Mycobacterium wolinskyi DSM 44493 T (98.7% sequence similarity), Mycobacterium mageritense DSM 44476 T (98.5%), and Mycobacterium palauense DSM 44914 T (98.3%). However, whole-genome-based analyses revealed that the strain is more closely related to M. celeriflavum, M. komaniense, M. novocastrense, and M. malmesburyense, with average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values well below the species delineation thresholds (ANI < 85%, dDDH < 30%). The complete genome of strain ND9-15 T comprises a circular chromosome of 5.1 Mb with a DNA G + C content of 66.9% and 4,650 predicted coding sequences. Chemotaxonomic analysis identified C₁₆:₀, C₁₈:₀ 10-methyl (TBSA), and summed feature 7 (C₁₉:₁ ω7c/C₁₉:₁ ω6c/C₁₉:₀ cyclo ω10c) as major fatty acids. The predominant polar lipids were phosphatidylethanolamine, phosphatidylinositol, and diphosphatidylglycerol. Biochemically, strain ND9-15 T was differentiated from its closest relatives by its protease activity, and lack of nitrate reduction and urease activity. Based on its phenotypic, chemotaxonomic, and phylogenetic characteristics, strain ND9-15 T is proposed as the type strain of a novel species, Mycobacterium jinjuensis sp. nov.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 12","pages":"550"},"PeriodicalIF":2.6,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145279198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clostridium chauvoei Antigens: Applications, Available Options and Potential Alternatives in the Future. 萧氏梭状芽孢杆菌抗原：应用、现有选择和未来的潜在替代品。

IF 2.6 3区生物学

Current Microbiology Pub Date : 2025-10-13 DOI: 10.1007/s00284-025-04554-x

Azadeh Zahmatkesh

{"title":"Clostridium chauvoei Antigens: Applications, Available Options and Potential Alternatives in the Future.","authors":"Azadeh Zahmatkesh","doi":"10.1007/s00284-025-04554-x","DOIUrl":"https://doi.org/10.1007/s00284-025-04554-x","url":null,"abstract":"Clostridium chauvoei is a gram-positive spore-forming anaerobic bacterium and the cause of blackleg, a highly fatal endogenous disease in ruminants. The bacterium produces both cellular (somatic and flagellar) and soluble antigens (toxins) that contribute to the pathogenesis. Immunity to C. chauvoei is primarily anti-bacterial, and so the traditional vaccines are formalinized whole-culture bacterins. The current research focuses on identifying specific antigens as immuno-protective antigens including cell surface or flagellar proteins or soluble antigens. This paper summarizes the application of C. chauvoei immunogens for detection or vaccine quality control, and reviews the ongoing research into developing more targeted subunit vaccines using recombinant or purified antigens like CctA or flagellin. Despite high genomic similarity in virulence factors among C. chauvoei strains, differences in the fliC gene encoding flagellin exist. Hence, strain differences may affect the protection efficacy against Blackleg disease. The development of effective Blackleg subunit vaccines necessitates careful consideration of the C. chauvoei strain, antigen purification techniques, bacterial growth phase and the preservation of crucial conformational epitopes in recombinant technology.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 12","pages":"551"},"PeriodicalIF":2.6,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145279125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biocontrol Effects and Potential Defence Mechanism of Bacillus velezensis JS6-1 Against Fusarium solani-Induced Postharvest Rots in Muskmelon. velezensis JS6-1对甜瓜采后枯萎病的生防效果及潜在防御机制

IF 2.6 3区生物学

Current Microbiology Pub Date : 2025-10-13 DOI: 10.1007/s00284-025-04522-5

Yafang He, Jiayi Wei, Fushuang Chen, Ning Wang, Yajie Han, Zifei Wu, Xingwen Wang, Faqiang Zhan, Yingwu Shi, Rong Yang, Huifang Bao

{"title":"Biocontrol Effects and Potential Defence Mechanism of Bacillus velezensis JS6-1 Against Fusarium solani-Induced Postharvest Rots in Muskmelon.","authors":"Yafang He, Jiayi Wei, Fushuang Chen, Ning Wang, Yajie Han, Zifei Wu, Xingwen Wang, Faqiang Zhan, Yingwu Shi, Rong Yang, Huifang Bao","doi":"10.1007/s00284-025-04522-5","DOIUrl":"https://doi.org/10.1007/s00284-025-04522-5","url":null,"abstract":"The purpose of this study was to examine the biocontrol effects and potential defence mechanism of Bacillus velezensis JS6-1 in controlling rot in muskmelons caused by Fusarium solani. The results indicated that Bacillus velezensis JS6-1 could directly inhibit the growth of Fusarium solani mycelium and demonstrated high stability of antibacterial activity under varying temperatures, pH levels, and UV exposure times. The volatile organic compounds (VOCs) produced by Bacillus velezensis JS6-1 and the lipopeptide and protein extracts derived from the fermentation broth of Bacillus velezensis JS6-1 both exhibited broad antagonistic activity. Genes associated with the synthesis of the antimicrobial substances iturin, fengycin, and surfactin were identified in the lipopeptide extract obtained from JS6-1. Subsequently, the protein extract from the fermentation broth of Bacillus velezensis JS6-1 was identified, revealing 12 proteins associated with antibacterial activity. Consequently, we applied the Bacillus velezensis JS6-1 for the control of muskmelon decay caused by Fusarium solani. Under greenhouse conditions, the Bacillus velezensis JS6-1 fermentation broth was able to inhibit the decay of muskmelon caused by Fusarium solani. Additionally, the strain enhanced the activity of superoxide dismutase (SOD), peroxidase (POD), and phenylalanine ammonia lyase (PAL) in the fruit, thereby strengthening the fruit's defensive capabilities. In summary, this strain has the potential as a biological control agent for managing postharvest rot.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 12","pages":"553"},"PeriodicalIF":2.6,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145279139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence and Antimicrobial Resistance of Klebsiella pneumoniae Isolated from Subclinical Mastitis in Selected Pure Dairy Cattle Breeds in Pakistan. 巴基斯坦部分纯奶牛亚临床乳腺炎分离肺炎克雷伯菌的流行及耐药性研究

IF 2.6 3区生物学

Current Microbiology Pub Date : 2025-10-12 DOI: 10.1007/s00284-025-04550-1

Sanam, Ijaz Ul Haq, Mustafa Kamal, Shehryar Khan, Irfan Khattak, Naimat Ullah Khan, Tariq Ali, Sana Riaz, Salvatore Massa, Tahir Usman

{"title":"Prevalence and Antimicrobial Resistance of Klebsiella pneumoniae Isolated from Subclinical Mastitis in Selected Pure Dairy Cattle Breeds in Pakistan.","authors":"Sanam, Ijaz Ul Haq, Mustafa Kamal, Shehryar Khan, Irfan Khattak, Naimat Ullah Khan, Tariq Ali, Sana Riaz, Salvatore Massa, Tahir Usman","doi":"10.1007/s00284-025-04550-1","DOIUrl":"https://doi.org/10.1007/s00284-025-04550-1","url":null,"abstract":"Bovine mastitis caused by Klebsiella pneumoniae poses significant economic and health challenges in dairy production, particularly in its subclinical form. In this study, milk samples (n = 305) from different cattle breeds of Red Sindhi, Sahiwal, Cholistani, and Holstein Friesian were collected from six dairy farms in Pakistan. The prevalence of subclinical mastitis ranged from 42.3% in Red Sindhi to 77.0% in Sahiwal breeds, with an overall prevalence of 56.39%. The prevalence of K. pneumoniae was 11.8% (36/305), varying significantly among breeds: Cholistani (26.2%), Red Sindhi (17.3%), Holstein Friesian (5.0%), and Sahiwal (0%). Farm-specific prevalence ranged from 0% to 26.2%, underscoring the influence of local management practices. All tested microorganisms remained sensitive to cefotaxime, meropenem, and ciprofloxacin but revealed 100% resistance to ampicillin and 80.5% and 69.4% resistance to amoxicillin and streptomycin, respectively. Molecular analysis detected the blaTEM in 91.6% of isolates. These findings highlight significant breed and farm-level differences in subclinical mastitis and resistance patterns. The predominance of blaTEM and full susceptibility to third-generation cephalosporins suggest that local K. pneumoniae isolates may differ from global trends. Strengthened farm management and prudent antimicrobial use are essential to limit infections and safeguard both animal and public health.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 12","pages":"548"},"PeriodicalIF":2.6,"publicationDate":"2025-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145273919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Endophytic Bacillus inaquosorum and Bacillus safensis Enhanced Growth, Proline Content and Antioxidant Enzymes in Avena sativa L. Under Salt Stress. 盐胁迫下玉米内生芽孢杆菌和萨芬芽孢杆菌对玉米生长、脯氨酸含量和抗氧化酶的影响

IF 2.6 3区生物学

Current Microbiology Pub Date : 2025-10-12 DOI: 10.1007/s00284-025-04536-z

Arshdeep Kaur, Pratibha Vyas, Namarta Gupta, Rahul Kapoor, Loveena Pathak

{"title":"Endophytic Bacillus inaquosorum and Bacillus safensis Enhanced Growth, Proline Content and Antioxidant Enzymes in Avena sativa L. Under Salt Stress.","authors":"Arshdeep Kaur, Pratibha Vyas, Namarta Gupta, Rahul Kapoor, Loveena Pathak","doi":"10.1007/s00284-025-04536-z","DOIUrl":"https://doi.org/10.1007/s00284-025-04536-z","url":null,"abstract":"The study aimed at selecting salt-tolerant endophytic bacteria with the ability to improve growth, proline content, and antioxidant enzymes in Avena sativa (Oat) under salinity. Of 43 endophytic bacteria isolated from various Oat tissues on nutrient agar with 2.5% NaCl, all produced ammonia and auxins. Additionally, 30.2% solubilized phosphate, 83.7% solubilized ZnO, 4.6% solubilized Zn3(PO4), 7.0% solubilized ZnCO3, 69.8% produced siderophores, and 2.3% produced HCN. These isolates also differed in their ability to exhibit growth at different NaCl levels (5-15%). Dendrogram that was generated based on plant growth-promoting traits and salinity tolerance exhibited three clusters and four independent branches. Out of thirteen bacterial isolates selected based on plant growth-promoting traits and salinity tolerance, five isolates showed significantly higher radicle and plumule length of Oat over control on water agar plates. Two promising isolates evaluated for growth promotion, antioxidant, and osmoprotectant activities in two salt-resistant (OL-14, OL-1876-2) and two susceptible (UPO-212, OL-1971) Oat genotypes under salinity in pots significantly enhanced plant height, dry weight, proline content, superoxide dismutase, peroxidase, and catalase activities over the uninoculated control. The SEM analysis showed the colonization of plant tissues by these bacterial strains validating their endophytic nature. Isolates were characterized based on polyphasic approach exhibiting maximum similarity to Bacillus inaquosorum O3SE2 and Bacillus safensis O4SE1. Salinity-tolerant plant growth-promoting B. inaquosorum and B. safensis as endophytes associated with Avena sativa L. are reported for the first time.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 12","pages":"549"},"PeriodicalIF":2.6,"publicationDate":"2025-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145273924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic and Pan-Genomic Characterization of Elizabethkingia meningoseptica Reveals Resistance Gene Profiles, Phylogenetic Clusters, and Functional Landscape. elizabeth ethkingia meninseptica的基因组和泛基因组特征揭示了抗性基因谱、系统发育集群和功能景观。

IF 2.6 3区生物学

Current Microbiology Pub Date : 2025-10-11 DOI: 10.1007/s00284-025-04548-9

Zheng Zhou, Wenqing Xiang, Chao Fang, Hongqiang Shen

{"title":"Genomic and Pan-Genomic Characterization of Elizabethkingia meningoseptica Reveals Resistance Gene Profiles, Phylogenetic Clusters, and Functional Landscape.","authors":"Zheng Zhou, Wenqing Xiang, Chao Fang, Hongqiang Shen","doi":"10.1007/s00284-025-04548-9","DOIUrl":"https://doi.org/10.1007/s00284-025-04548-9","url":null,"abstract":"Elizabethkingia meningoseptica (E. meningoseptica) is a significant pathogen associated with various infections. This study aimed to analyze the general molecular and pan-genomic characteristics of E. meningoseptica. The 104 E. meningoseptica genomes were retrieved from NCBI, annotated using Prokka, and screened for antibiotic resistance genes via ABRicate. Core-genome single-nucleotide polymorphisms (SNPs) were identified using Parsnp, with phylogenetic trees constructed. Pan-genomic analysis employed BPGA for gene clustering at 80% identity, supplemented by COG and KEGG functional annotations. Among the 104 sequences, 86 were from human sources, 12 from nonhuman sources, and 6 had unknown origins. Phylogenetic analysis classified the strains into three clades (A, B, and C), with 95 strains (91.3%) in clade C. Nineteen resistance genes were identified, with 11 being carbapenem-resistance genes. Significant differences in the distribution of resistance genes were observed between human and non-human sources. Pan-genomic analysis revealed 8856 genes in total, including 2267 core genes, 4052 accessory genes, and 2537 unique genes. The median genomic G + C content was 36.9% (36.8%-37.0%). The pan-genome was still open but approaching closure. In COG functional classification, significant differences were found in \"Information storage and processing\", \"cellular processes and signaling\" and \"Metabolism\" between core genes and accessory/unique genes. Similar significant differences were also observed in KEGG functional classification, especially in Cellular Processes, Human Diseases, and Metabolism. This study provides valuable insights into the genomic characteristics of E. meningoseptica, which can contribute to understanding its evolution, pathogenicity, and drug resistance mechanisms.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 12","pages":"546"},"PeriodicalIF":2.6,"publicationDate":"2025-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145273958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Accuracy of SAM-TB Platform as a Diagnostic Assay for Detection of Mycobacterium tuberculosis Drug Resistance Versus TB-Proflier, PhyResSE, and Mykrobe: A Head-to-Head Comparison. SAM-TB平台作为检测结核分枝杆菌耐药性与TB-Proflier、PhyResSE和Mykrobe的诊断方法的准确性：一项正面比较

IF 2.6 3区生物学

Current Microbiology Pub Date : 2025-10-11 DOI: 10.1007/s00284-025-04530-5

Juexin Wang, Xiaoling Lai, Chuchu Wu, Jielian Wang, Yong Liao, Qiong Lei, Zhenbo Xu

{"title":"Accuracy of SAM-TB Platform as a Diagnostic Assay for Detection of Mycobacterium tuberculosis Drug Resistance Versus TB-Proflier, PhyResSE, and Mykrobe: A Head-to-Head Comparison.","authors":"Juexin Wang, Xiaoling Lai, Chuchu Wu, Jielian Wang, Yong Liao, Qiong Lei, Zhenbo Xu","doi":"10.1007/s00284-025-04530-5","DOIUrl":"https://doi.org/10.1007/s00284-025-04530-5","url":null,"abstract":"Accurate detection of drug-resistant Mycobacterium tuberculosis (MTB) is essential for tuberculosis control, particularly with increasing multidrug-resistant strains. Whole-genome sequencing (WGS), as a supplement to phenotypic drug susceptibility testing (DST), holds great potential for resistance detection, but comparative analyses of multiple WGS tools remain limited. This study assessed four WGS-based tools-SAM-TB, TB-Profiler, Mykrobe predictor, and PhyResSE-for predicting resistance to four first-line drugs (isoniazid, rifampicin, ethambutol, and streptomycin) using 113 clinical MTB isolates from Ganzhou, China, with phenotypic DST as reference. All tools showed similar sensitivity (85.04-88.19%) and high specificity (95.69-96.31%). Several known resistance-associated mutations were identified, along with seven novel mutations absent from the WHO catalogue and thirteen of uncertain clinical significance. Discrepancies between WGS and DST occurred across all drugs, potentially due to disputed mutations, outdated databases, or limitations of phenotypic methods. These results highlight the complementary roles of WGS and phenotypic DST in drug resistance surveillance and emphasize the need for continuously updated mutation databases, including the WHO catalogue, and standardized interpretation criteria to enhance the clinical utility of WGS.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 12","pages":"547"},"PeriodicalIF":2.6,"publicationDate":"2025-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145273960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Description of Tateyamaria algicola sp. nov. and Tateyamaria rhodophyticola sp. nov., Isolated from Marine Algae. 海藻中分离的褐藻和红藻褐藻的描述。

IF 2.6 3区生物学

Current Microbiology Pub Date : 2025-10-09 DOI: 10.1007/s00284-025-04537-y

Byeong Jun Choi, Jeong Min Kim, Hülya Bayburt, Dae Gyu Choi, Seo Hui Choi, Che Ok Jeon

{"title":"Description of Tateyamaria algicola sp. nov. and Tateyamaria rhodophyticola sp. nov., Isolated from Marine Algae.","authors":"Byeong Jun Choi, Jeong Min Kim, Hülya Bayburt, Dae Gyu Choi, Seo Hui Choi, Che Ok Jeon","doi":"10.1007/s00284-025-04537-y","DOIUrl":"https://doi.org/10.1007/s00284-025-04537-y","url":null,"abstract":"Two aerobic, non-motile, Gram-stain-negative strains, designated SN3-11ᵀ and SN6-1ᵀ, were isolated from marine algae. Both strains were oxidase- and catalase-positive, with rod-shaped morphology. Strain SN3-11ᵀ grew optimally at 25 ℃, pH 7.0-8.0, and 2.0-5.0% (w/v) NaCl, while strain SN6-1ᵀ showed optimal growth at 25 ℃, pH 8.0, and 2.0-3.0% NaCl. Both strains contained ubiquinone-10 as the sole respiratory quinone, and their major fatty acids were summed feature 8 (C18:1 ω7c and/or C18:1 ω6c), C16:0, and C19:0 cyclo ω8c. Phosphatidylcholine and phosphatidylglycerol were dominant polar lipids, with diphosphatidylglycerol additionally present in SN3-11ᵀ. Strains SN3-11ᵀ and SN6-1ᵀ had genome sizes of 4,762 Kb and 4,157 Kb with G + C contents of 61.3% and 62.0%, respectively. They shared 96.9% 16S rRNA gene sequence similarity, 77.0% average nucleotide identity (ANI), and 20.2% digital DNA-DNA hybridization (dDDH), supporting their classification as distinct species. Their closest relative, Tateyamaria armeniaca KMU-156ᵀ, exhibited 16S rRNA gene sequence similarities of 98.6% to SN3-11ᵀ and 97.7% to SN6-1ᵀ. Phylogenetic trees based on 16S rRNA and whole-genome sequences placed both strains in distinct lineages within Tateyamaria. ANI and dDDH values between the two isolates and other Tateyamaria species were < 78.5% and < 21.0%, respectively. Strains SN3-11ᵀ and SN6-1ᵀ harbored genes encoding diverse carbohydrate-active enzymes and biosynthetic pathways for lycopene, pantothenate, and riboflavin, suggesting potential symbiotic roles with algal hosts. Based on phenotypic, chemotaxonomic, and genomic characteristics, SN3-11ᵀ and SN6-1ᵀ represent two novel species, Tateyamaria algicola sp. nov. (SN3-11ᵀ = KACC 23689ᵀ = JCM 36649ᵀ) and Tateyamaria rhodophyticola sp. nov. (SN6-1ᵀ = KACC 23127ᵀ = JCM 35962ᵀ).","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 11","pages":"545"},"PeriodicalIF":2.6,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145250246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Higher Variability in Diversity and Functionality of Soil Fungal Communities Compared to Bacterial Communities in Alpine Treeline Ecotone. 高寒乔木带土壤真菌群落多样性和功能差异高于细菌群落。

IF 2.6 3区生物学

Current Microbiology Pub Date : 2025-10-09 DOI: 10.1007/s00284-025-04547-w

Yihua Ren, Shoutian Ma, Jie Lu, Qisheng Han, Zhaojun Liu

{"title":"Higher Variability in Diversity and Functionality of Soil Fungal Communities Compared to Bacterial Communities in Alpine Treeline Ecotone.","authors":"Yihua Ren, Shoutian Ma, Jie Lu, Qisheng Han, Zhaojun Liu","doi":"10.1007/s00284-025-04547-w","DOIUrl":"https://doi.org/10.1007/s00284-025-04547-w","url":null,"abstract":"Although patterns of microbial diversity in forest ecotones have been well-studied, the association between microbial diversity and function in treeline zone remain largely unexplored. This study examined the soil microbial diversity and community structure along altitudinal gradient (High, Medium, Low) and at different soil depths (10 cm, 20 cm and 30 cm) of the Abies georgei var. smithii forest in a typical ecotone area of treeline on Sygera Mountains in southeastern Tibet. Results indicated that the alpha diversity of bacterial communities is highest in the medium elevation, while fungal diversity is lowest. Additionally, both bacterial and fungal diversity decrease with increasing soil depth. Bacterial community structure is significantly influenced by both elevation and depth. Fungal community structure is primarily affected by the elevation. Network analysis revealed that microbial functionality is more closely related to these communities than microbial biodiversity in-dices. In the treeline zone, the functional differences among fungi across various elevations and soil depths are significantly greater compared to those of bacteria.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 11","pages":"544"},"PeriodicalIF":2.6,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145250271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unraveling Diversity in Alternaria brassicae Affecting Indian mustard: Morphological, Pathogenic and Molecular Approaches. 揭示影响印度芥菜的油菜互花孢菌多样性：形态学、病原学和分子方法。

IF 2.6 3区生物学

Current Microbiology Pub Date : 2025-10-08 DOI: 10.1007/s00284-025-04521-6

Basavaraj A Dodmani, Lakshman Prasad, Naveen Singh, Shabnam Katoch, Mahender Singh Saharan

{"title":"Unraveling Diversity in Alternaria brassicae Affecting Indian mustard: Morphological, Pathogenic and Molecular Approaches.","authors":"Basavaraj A Dodmani, Lakshman Prasad, Naveen Singh, Shabnam Katoch, Mahender Singh Saharan","doi":"10.1007/s00284-025-04521-6","DOIUrl":"https://doi.org/10.1007/s00284-025-04521-6","url":null,"abstract":"Alternaria blight, caused by Alternaria brassicae (Berk.) Sacc. stands as one of the most prevalent and damaging disease afflicting mustard (Brassica juncea) cultivation on a global scale. In the current investigation, Alternaria infected leaf samples were collected from twenty-one distinct locations across eleven states of India. Isolates were comprehensively analyzed for genetic diversity through morphological variability, pathogenicity and molecular phylogeny studies. The results showed significant variation in cultural characteristics and radial growth among A. brassicae isolates, with growth ranging from 59.80 mm in Ab17 to 88.58 mm in Ab19. Pathogenicity data revealed that there were 5 highly virulent, 13 moderately virulent, and 3 least virulent isolates which was confirmed in digital image analysis of diseased spots on leaves using MATLAB's Image Processing Toolbox. This suggests the evolutionary potential of A. brassicae and its ability to adapt to different geographical regions. Based on sequencing of amplified ITS region and β-tubulin gene, all the isolates showed 100 per cent homology to the sequences of A. brassicae strain J3 (GenBank CM016504.1). Furthermore, DNA fingerprinting pattern generated by ISSR markers, all the isolates were individually grouped into 3 main clusters. Comparative analysis established congruence between DNA finger prints, virulence pattern, location and morphological grouping of A. brassicae isolates. The present research holds significance in precisely categorizing the pathogen responsible for crop infection, enabling us to develop a targeted and efficient strategy for future disease control measures.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 11","pages":"542"},"PeriodicalIF":2.6,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145250274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0