Current Microbiology最新文献

{"title":"Novel Insights into the Antibacterial Potential of Euphrasia officinalis L.","authors":"Marina M Stanković, Petar M Ristivojevic, Đurđa D Ivković, Jelena N Terzić, Olgica D Stefanović","doi":"10.1007/s00284-025-04399-4","DOIUrl":"https://doi.org/10.1007/s00284-025-04399-4","url":null,"abstract":"<p><p>Regarding the resistance of bacteria to conventional antibiotics, the search for novel natural antibacterial agents is of significant importance. Herein, the antibacterial activity of ethanol and acetone extracts of the medicinal plant Euphrasia officinalis was evaluated. For the first time, the optimization of ultrasound-assisted extraction conditions to achieve a higher concentration of polyphenols and increase the antibacterial activity of E. officinalis extracts was performed using response surface methodology. Polyphenolic compounds were quantified through spectrophotometric and chromatographic techniques. The optimized extracts' antibacterial activity was analyzed via broth microdilution, time-kill, and cell membrane permeability assays. The optimized extracts exhibited strain-dependent antibacterial activity. Gram-positive bacteria (Staphylococcus aureus LM11, S. aureus LM12, and Enterococcus sp. LM5) were the most susceptible, at an extract concentration of 10 mg/mL. Tested extracts demonstrated a bactericidal effect after 24 h. Additionally, the extracts impact the cell membrane's permeability, disrupting its normal function which is the first report on the potential mode of action of tested E. officinalis extracts. Major compounds identified in optimized extracts were rutin (9.52 mg/kg dry weight (DW), ellagic acid (7.1 mg/kg DW), quercetin 3-O-glucoside (4.78 mg/kg DW), and isorhamnetin 3-O-rutinoside (3.21 mg/kg DW). This study provides a comprehensive evaluation of the extraction process, phytochemical composition, and antibacterial properties of E. officinalis extracts, laying the basis for developing new applications of these extracts as natural antibacterial agents.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 9","pages":"409"},"PeriodicalIF":2.3,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144706796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biological Decontamination of Mycotoxigenic Fusarium verticillioides Using Lactiplantibacillus plantarum MYSN128 Isolated from Traditional Vellappam: Implications for Food Safety and Global Mycotoxin Management.","authors":"G Adithi, Rakesh Somashekaraiah, B Shruthi, S Divyashree, N Deepa, P R Vanitha, R Vasundaradevi, B V Deepthi, M Y Sreenivasa","doi":"10.1007/s00284-025-04401-z","DOIUrl":"https://doi.org/10.1007/s00284-025-04401-z","url":null,"abstract":"<p><p>Fungal deterioration of food and feed commodities by mycotoxigenic fungi has become a significant complication worldwide. Fusarium contamination produces extremely noxious mycotoxins such as Fumonisin B1 (FB1) and Fumonisin B2 (FB2), which affect human and animal well-being. The present work explored the fungicidal activities of probiotic bacteria isolated from the traditional fermented food Vellappam. About 20 isolates were obtained from the sample and subjected to initial screening against Fusarium verticillioides. Out of 20, Lactiplantibacillus plantarum MYSN128 exhibited potent antifungal activity against F. verticillioides. Moreover, the isolate MYSN128 was found to survive in acidic and bile environments, but it was susceptible to the tested antibiotics. The isolate displayed 61% autoaggregation, 59.52% cell surface hydrophobicity, and chicken crop epithelial cell adherence. L. plantarum MYSN128 and its CFS affected the growth of conidia, resulting in deformed and damaged conidia. A cell-free supernatant (CFS) at a 20% concentration efficiently reduced mycelial biomass to 0.269 ± 0.11 g, compared to the control at 1.938 ± 0.15 g. Similarly, conidial germination was repressed to 19.12% ± 1.15, and a reduction in F. verticillioides growth was observed on maize seeds treated with CFS after 7 days of incubation. The internal transcribed spacer (ITS) copy number of F. verticillioides declined to 5.23 × 10⁷ and 8.026 × 10⁷ in the presence of L. plantarum MYSN128 and its CFS, respectively, in comparison to 8.940 × 10¹⁰ copies in the control. The damaged and dead spores were stained with propidium iodide, which resulted in a reduction in the live population of F. verticillioides observed after treatment with CFS of MYSN128 in flow cytometric analysis. The isolate was also effective in reducing poultry feed contamination. Hence, our results suggest that L. plantarum MYSN128 possesses capable probiotic characteristics and exhibits prominent antifungal activity, which can be utilized as a preservative for food and feed.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 9","pages":"404"},"PeriodicalIF":2.3,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144697866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oryzifoliimicrobium ureilyticus gen. nov., sp. nov., a Novel Phosphate-Solubilizing and Siderophore-Producing Bacterium Within the Family Rhizobiaceae.","authors":"Guo-Hong Liu, Ling Yao, Peng Gao, Shu-Qun Zhang, Bing Li, Christopher Rensing, Wen-Qing Li, Shun-Gui Zhou","doi":"10.1007/s00284-025-04376-x","DOIUrl":"https://doi.org/10.1007/s00284-025-04376-x","url":null,"abstract":"<p><p>A Gram-stain-negative, rod-shaped, strictly aerobic bacterium strain SG148^T, within the family Rhizobiaceae, was isolated from rice leaves collected in Fujian Province, China. Phylogenetic analysis utilizing 16S rRNA gene sequences revealed that strain SG148^T displayed a high sequence similarity of 95.2-95.8% to the strains of closely related species of Shinella and Rhizobium. The highest digital DNA-DNA hybridization (dDDH), core-proteome average amino acid identity (cpAAI), and average nucleotide identity (ANI) values between strain SG148^T and related strains were estimated as 20.7%, 79.3%, and 73.7%, respectively. The predominant respiratory quinones in strain SG148^T were identified as Q-9 and Q-10, while major polar lipids included diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, and phosphatidylethanolamine. Additionally, strain SG148^T was capable of phosphate-solubilizing and siderophore-producing carrier, significantly promoting rice seedling growth. The DNA G + C content was determined to be 56.1%. Given its distinct phylogenetic and phenotypic characteristics, strain SG148^T is considered to represent a novel genus, for which the name Oryzifoliimicrobium ureilyticus gen. nov., sp. nov. is proposed. The type strain is SG148^T (= MCCC 1K08901^T = KCTC 8337^T).</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 9","pages":"406"},"PeriodicalIF":2.3,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144697879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Purification and Characterization of Antimicrobial Peptide from an Epilithic Bacterium Streptomyces sp. AL50 with Activity Against Biofilm Forming Coagulase-Negative Staphylococci.","authors":"Santa Ram Joshi, Kaushik Bhattacharjee","doi":"10.1007/s00284-025-04407-7","DOIUrl":"https://doi.org/10.1007/s00284-025-04407-7","url":null,"abstract":"<p><p>Antimicrobial peptide (AMP) is increasingly recognized as a promising new avenue in addressing the challenge of antibiotic resistance, offering a notable alternative to traditional antibiotic molecules. The current research focuses on extracting and characterizing AMP named as AMP1 from epilithic bacterial isolate Streptomyces sp. AL50, an area that has so far remained underexplored. The AMP1 was purified by ammonium sulfate precipitation, dialysis, and sephadex G-100 column chromatography. Subsequent analysis using Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed major band with molecular mass of approximately 10.83 kDa. The peptide demonstrated potent activity against Staphylococcus epidermis MTCC3615, with MIC value of 4 μg/mL. Remarkably, the peptide retained its activity up to 70 °C temperature and within a pH range of 6.0-8.0. However, its activity was abolished by proteinase K, trypsin, and papain, while pepsin, lipase, and α-amylase had no effect. Furthermore, AMP1 displayed notable capabilities in inhibiting and eradicating biofilms. These results indicate that AMP1 hold significant potential as antibiotic candidates for treating multidrug-resistant bacterial infections.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 9","pages":"407"},"PeriodicalIF":2.3,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144697880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic and Phenotypic Characterization of Pseudomonas juntendi Strain A3e, a Drug-Resistant Isolate from Irrigation Water.","authors":"Ángel Ibarra-Rodríguez, Jean Pierre González-Gómez, Manuel Ángel Castillo-Olea, Bruno Gomez-Gil, Nohelia Castro-Del Campo, Cristobal Chaidez","doi":"10.1007/s00284-025-04371-2","DOIUrl":"https://doi.org/10.1007/s00284-025-04371-2","url":null,"abstract":"<p><p>Pseudomonas juntendi is a newly recognized opportunistic pathogen often exhibiting multi-drug resistance and commonly isolated from clinical settings. Here, we report the characterization of P. juntendi strain A3e, isolated from irrigation water, which was analyzed using antimicrobial susceptibility testing and whole-genome sequencing. Taxonomic classification via multi-locus sequence typing and average nucleotide identity analysis confirmed the strain's identity as P. juntendi with over 99% similarity. Phenotypic testing revealed strong biofilm formation and resistance to four antibiotics and intermediate resistance to two additional antibiotics. Genomic analysis identified 24 antibiotic-resistance genes, predominantly related to efflux pumps, and 50 virulence-related genes involved in adhesion, motility, biofilm formation, and siderophore uptake. This is the first genome of P. juntendi reported in Mexico; despite its environmental origin, the potential clinical implications warrant attention.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 9","pages":"403"},"PeriodicalIF":2.3,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144697878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in the Relationship Between Skin Tumor Occurrence, Development, Prognosis, and the Human Microbiome.","authors":"Yuchen Zhao, Xinyuan Liu, Peijun Song, Qi Chen, Xiaodong Yang, Wenyuan Yu","doi":"10.1007/s00284-025-04390-z","DOIUrl":"https://doi.org/10.1007/s00284-025-04390-z","url":null,"abstract":"<p><p>The human microbiome, including bacteria, fungi, and viruses, is widely distributed on the skin, in the oral cavity, in the gastrointestinal tract, and is also present in tumors. It is closely associated with the occurrence, development, and treatment of skin cancers and contributes to the formation of the tumor microenvironment. This review provides a detailed overview of the composition and origin of microorganisms in different types of skin tumors, focuses on the roles and mechanisms of microbiome in skin cancer, discusses their applications in the treatment of skin tumors, and outlines future research directions.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 9","pages":"408"},"PeriodicalIF":2.3,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144697865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the Plant Growth-Promoting Potential of a Purple Non-Sulfur Bacterium: Cereibacter sphaeroides PW15.","authors":"Hamza Ettadili, Behiye Nur Aksoy, Caner Vural","doi":"10.1007/s00284-025-04384-x","DOIUrl":"https://doi.org/10.1007/s00284-025-04384-x","url":null,"abstract":"<p><p>Purple non-sulfur bacteria (PNSB) are phototrophic microorganisms recognized for their ability to produce compounds beneficial for plant growth, increasing their interest as plant growth-promoting bacteria. This study investigates the growth-promoting traits of Cereibacter sphaeroides PW15 under both microaerobic light and aerobic dark conditions. The findings demonstrate that the PNSB isolate PW15 exhibits notable salt tolerance, phosphate solubilization, nitrogen fixation, and the production of several plant-beneficial metabolites, including indole-3-acetic acid, ammonia, siderophores, and 5-aminolevulinic acid. The presence of the nifH gene, which is associated with Mo-Fe nitrogenase, further confirmed its nitrogen-fixing potential. Additionally, C. sphaeroides PW15 formed biofilm on abiotic surfaces and significantly enhanced root and shoot growth in Arabidopsis thaliana ecotype Col-0 upon inoculation. These results highlight the potential of C. sphaeroides PW15 as a promising candidate for use as an effective biofertilizer in sustainable agriculture.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 9","pages":"405"},"PeriodicalIF":2.3,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144697877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lacticaseibacillus paracasei Glu-07: A Promising Probiotic Candidate for the Management of Type 2 Diabetes Mellitus.","authors":"Wenyi Xu, Yinghua Guan, Liping Tian, Yu Fu, Yinghui Zhang, Yanhong Liu, Yanfei Hu, Bowen Zhao, Chongming Wu","doi":"10.1007/s00284-025-04391-y","DOIUrl":"https://doi.org/10.1007/s00284-025-04391-y","url":null,"abstract":"<p><p>The global prevalence of type 2 diabetes mellitus (T2DM) is currently surging, posing significant health and socioeconomic burdens. Probiotics have emerged as promising interventions due to their safety and potential metabolic benefits. Gut microbiota plays a critical role in the pathophysiology of metabolic diseases in humans, offering valuable insights for therapeutic strategies such as probiotics. Here, aiming to identify novel probiotics from the human gut microbes to combat T2DM, a total of 203 human intestinal bacterial strains were screened in vitro to evaluate their enzymatic inhibition of α-glucosidase and α-amylase, in which Lacticaseibacillus paracasei Glu-07 (= QH-142 = CGMCC 23796) exhibited the highest inhibitory efficacy (81.05% and 72.56% for α-glucosidase and α-amylase, respectively). In HepG2 cells, L. paracasei Glu-07 elevated glucose uptake and consumption by 22.4% and 85.8%. Oral administration of strain Glu-07 in diabetic mice significantly improved hyperglycemia, hyperlipidemia, and restored the structure and composition of gut microbiota toward a healthier profile. Mechanistically, strain Glu-07 activated the AMPK-AKT1-GSK3β/FOXO1 signaling pathways by modulating the expression of key genes, enhanced glycolysis and fatty acid ꞵ-oxidation, and inhibited gluconeogenesis and lipogenesis. It also stimulated GLP-1 secretion, potentially via triggering the GPR41/43-GLP-1 axis, and increased the abundance of beneficial gut microbes, such as Akkermansia muciniphila (~ threefold). The dual regulations synergistically support strain Glu-07 as a promising probiotic candidate for T2DM management.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 9","pages":"402"},"PeriodicalIF":2.3,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144689562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibiotic Resistance and Molecular Epidemiology of ESBL-Producing E. coli in Shrimp Aquaculture: A Study from Tiruvallur District, Tamil Nadu, India.","authors":"Gururaj Moorthy, Uma Arumugam","doi":"10.1007/s00284-025-04329-4","DOIUrl":"https://doi.org/10.1007/s00284-025-04329-4","url":null,"abstract":"<p><p>The rapid growth of aquaculture and rising demand for high-quality animal protein have resulted in increased usage of antimicrobials in fish farming, raising worries about the rise of antibiotic resistance. This study was carried out to determine the prevalence of ESBL-producing E. coli in shrimp farms of Tiruvallur district, Tamil Nadu, South India for a duration of 5 months from February 2022 to June 2022. A total of 102 samples from 22 shrimp farms were collected, including 51 shrimp, 10 soil, 37 water, and 4 feed, with 73 of 79 E. coli isolates (92.4%) producing ESBL. Three E. coli isolates positive for the stx2 gene were discovered in shrimp farms with high and medium salinity. Antibiotic resistance patterns found that chloramphenicol had the lowest rate of resistance (2.5%), while ampicillin had the highest rate of resistance (39.2%). Approximately 70% of the isolates were multidrug resistant. Molecular screening revealed that the blaTEM gene is the most prevalent beta-lactamase gene (39.4%), followed by blaCTX (27.3%), blaSHV (24.3%), and ampC (19.2%). Additionally, the sul1 gene was found in 8.1% of isolates. These findings suggest that the prevalence of E. coli with virulent characteristics and high level of contamination in fisheries pose a public health danger, potentially affecting consumer health as well as prawn export markets. The finding of multidrug-resistant E. coli in shrimp aquaculture emphasizes the importance of increased biosecurity and antibiotic stewardship in preventing contamination and protecting public health.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 9","pages":"401"},"PeriodicalIF":2.3,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144689561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbiome Assembly of Phyllody-Infected Sesame Leaves.","authors":"Sakthivel Krishnan, Charishma Krishnappa, Neelam Sheoran, Bhaskar Reddy, Aundy Kumar","doi":"10.1007/s00284-025-04383-y","DOIUrl":"https://doi.org/10.1007/s00284-025-04383-y","url":null,"abstract":"<p><p>Sesame phyllody disease poses a serious threat to sesame cultivation, often leading to complete crop failure due to the absence of resistant varieties and lack of effective chemical treatments. To explore sustainable alternatives, this study investigated the foliar microbiome by comparing microbial communities in healthy and phyllody-infected sesame plants from severely affected fields. Through combined culture-based techniques and DNA sequencing, we identified 3108 bacterial OTUs spanning several key genera, including Pantoea, Pseudomonas, Allorhizobium, and Xanthomonas. Phytoplasma, the known causal agent, was detected exclusively in symptomatic plants. Co-occurrence network analysis revealed distinct microbial associations: healthy plants showed balanced interactions with Pantoea, Pseudomonas, and Sphingomonas as central hubs, whereas infected plants exhibited a denser, more complex network, indicating phytoplasma-driven shifts in microbial structure. From culturable isolates, 72 bacterial strains were recovered, with Pantoea being predominant in both plant types. Functional predictions highlighted microbial association with nutrient metabolism and biosynthesis of alpha-linolenic acid, a naturally abundant compound in sesame. This first study of the sesame foliar microbiome provides valuable insights into plant-microbiome-phytoplasma interactions and lays a foundation for developing microbiome-based, eco-friendly strategies to manage sesame phyllody disease in the future.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 9","pages":"399"},"PeriodicalIF":2.3,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144674112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}