Current Microbiology最新文献

{"title":"Disaggregation of Colletotrichum truncatum by Peppermint Oil Nanoemulsion during Anthracnose Disease in Glycine max.","authors":"Navinit Kumar, Ashutosh Tripathi, Priyanka Chauhan, Pratibha Verma, Laxman Singh Rajput, Shipra Pandey, Pallavi Shukla, Raghvendra Singh, Sanjeev Kumar, Aradhana Mishra","doi":"10.1007/s00284-026-04933-y","DOIUrl":"https://doi.org/10.1007/s00284-026-04933-y","url":null,"abstract":"<p><p>Soybean (Glycine max), a protein- and oil-rich crop, supplies approximately 80% of oil requirements globally. The crop is susceptible to a wide variety of phytopathogens, among them Colletotrichum truncatum (CT) causes pre- and post-emergence damping-off in seeds and seedlings, leading to significant yield loss in the soybean crop. Here in this study, we assess the antifungal efficacy of peppermint oil nanoemulsion (PNE) against C. truncatum and also evaluate the disease suppression potential of PNE on soybean crop through seed priming. During the evaluation of fungicidal potential, 1% treatment of PNE reduces the growth of C. truncatum by 80% under in vitro conditions. PNE treatments induce oxidative stress by ROS accumulation, as suggested by Nitro Blue Tetrazolium (NBT) staining. Consequently, this redox imbalance affects the defence mechanism, cytosolic leakage and disruption of essential cellular processes leading to cell death. During PNE stress, stress enzyme activities were suppressed, including superoxide dismutase (SOD) by 26% and glutathione peroxidase (GPX) by 63%, while oxidative stress leads to enhanced lipid peroxidation by (LPX) 22% and polyphenol oxidase (PPO), 28%. Notably, scanning electron microscopy revealed significant aberration in hyphal structure, confirming the antifungal efficacy of PNE. Moreover, GC-MS analysis of ethyl acetate extracts from treated fungal cells identified 28 compounds, including thymol, menthol, phenyl alcohol, p-menthane, phenol, 1-docosanol, and 1-octadecene, present at elevated levels, related to antifungal activity. In addition, the nurturing effect of PNE was evaluated by a seed germination assay; PNE-treated seeds showed improved seed germination compared to Pathogen alone-infected samples. These findings suggest a potential role of PNE as an eco-friendly alternative for managing C. truncatum in soybean cultivation.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 6","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147812427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of Simulated Saline Alkaline Conditions on Soil Bacterial Community and Isolations.","authors":"Hong Yan, Yani Bai, Yuxin Lu, Zhizhen Feng, Wenjuan Zhao, Qian Xu","doi":"10.1007/s00284-026-04925-y","DOIUrl":"https://doi.org/10.1007/s00284-026-04925-y","url":null,"abstract":"<p><p>Saline-alkaline ions including Na⁺, Cl^-, HCO₃^-, and CO₃^2- etc., adversely impact agricultural production by decreasing soil adaptability and function of microbial communities. Until now, most of the studies regarding salinity were mainly focused on sodium chloride and sodium sulfate, seldom concerning the actual saline-alkaline soil conditions. In the current study, we collected saline-alkaline soil samples from three different habitats, and employed six simulated saline-alkaline conditions (encompassing sulfate-type, chloride-type, and soda-type) to enrich soil bacterial communities. Using high-throughput sequencing, we characterized the composition of bacterial communities under distinct enrichment conditions, and isolated strains from the respective soil environments via the single-colony isolation method. The results showed that, regardless of soil sources, bacterial community diversity decreased significantly under conditions where pH ≥ 9.0 and total salinity ≥ 6.0%. Among all tested factors, pH and carbonate ions were the most impactful in shaping bacterial community composition, followed by sodium chloride. This suggests that soda-type saline-alkaline soils exerted the strongest influence on microbial communities. Furthermore, specific microbial taxa responded differently to salt ion compositions. For example, Bacillus and Woeseia genus were positively associated with water-soluble chloride and sodium ions. Additionally, the keystone taxa among the enriched halotolerant bacterial isolates included Bacillus, Halomonas, Clostridioides, Pseudoalteromonas, Zobellella, and Alkaliphilus genus. From sulfate-type saline-alkaline soils, a greater number of bacterial isolates were recovered; however, no isolates could be enriched under condition 6 (pH 10, NaCl 10 g/L, Na₂SO₄ 5 g/L, Na₂CO₃ 5 g/L, salinity 2.0%). These results provided theoretical foundation and potential application guidance for the bio-remediation of saline-alkaline soils.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 6","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147812416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cupriavidus smyrnaeus sp. nov., Isolated from Soil Litter Near an Arum sp.","authors":"Nurettin Sahin, Akio Tani","doi":"10.1007/s00284-026-04932-z","DOIUrl":"https://doi.org/10.1007/s00284-026-04932-z","url":null,"abstract":"","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 6","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147765747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of Culture Media for Trichoderma Strains as a Sustainable Approach for Agriculture.","authors":"Isabela de L Valente, Luciana Luft, Giovani L Zabot, Marcio A Mazutti","doi":"10.1007/s00284-026-04921-2","DOIUrl":"10.1007/s00284-026-04921-2","url":null,"abstract":"<p><p>The intensive use of soil and the increased consumption of chemical fertilizers have risen over the years, causing soil degradation and impacting human health. The application of the fungus Trichoderma spp. has expanded exponentially and has been used to enhance soil fortification and detoxification of both soil and wastewater. Additionally, it has been applied in agricultural systems as a biocontrol agent against pathogens, to stimulate plant growth, and as a biological fertilizer to improve soil fertility. In this study, three Trichoderma strains (T. harzianum MMBF 58/09, T. asperellum URM 6997/160821, and T. harzianum IB 19/17) were cultivated using a culture medium designed through Plackett-Burman statistical design, followed by the characterization of the microorganisms to increase enzyme production, siderophore synthesis, and indole-3-acetic acid production. Significant enzymatic activity was observed, including chitinase (0.022 and 0.004 U·mL^- 1), β-1,3-glucanase (0.199 and 0.007 U·mL^- 1), and protease (1.45 and 1.40 U mL^- 1), as well as propagules (9.67 × 10⁷ conidia·mL^- 1, 2.56 × 10⁷ microsclerotia·mL^- 1, and 1.79 × 10⁷ chlamydospores·mL^- 1). Siderophore production reached 33.26%, and biomass was measured at 1.32 g·100 mL^- 1. Some assays of the strains MMBF 58/09, URM 6997/160,821, and IB 19/17 demonstrated that these strains have promising potential to contribute to food safety and agricultural quality. Therefore, the observed responses emphasize the need for oriented microbial selection, supporting the development of targeted bioinputs within the framework of applied microbiology.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 6","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13128744/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147765786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Descriptive Epidemiological Analysis of West Nile Virus Human Infections in Italy: A Surveillance-based Study (2018-2023).","authors":"Indraccolo Francesca, Mastria Michele, Chirizzi Daniela","doi":"10.1007/s00284-026-04938-7","DOIUrl":"https://doi.org/10.1007/s00284-026-04938-7","url":null,"abstract":"","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 6","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147765655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis of Diosgenin by Pseudomonas aeruginosa D3, an Endophyte of Dioscorea bulbifera.","authors":"Krishna Nanda Dhal, Pramod Kumar Sahu, Amrita Gupta, Adarsh Kumar, Zaryab Shafi, Parul Johri, Sushma Mishra, Alok Kumar Srivastava","doi":"10.1007/s00284-026-04926-x","DOIUrl":"https://doi.org/10.1007/s00284-026-04926-x","url":null,"abstract":"<p><p>Diosgenin, a steroid with multiple pharmacological properties such as anti-cancerous, anti-microbial, anti-inflammatory, cardioprotective, etc., is primarily derived from Dioscorea species, and represents an important component of steroidal medications. Due to its economic importance and rising demand, identifying sustainable alternative sources of diosgenin is imperative. Endophytes, a group of plant-associated microbial communities inhabiting the internal tissues of plants, have been reported to produce a wide range of bioactive compounds, including host metabolites. The study is aimed at bioprospecting of bacterial endophytes from Dioscorea bulbifera (air potato), an underutilized tuber crop collected from Chhattisgarh for the production of diosgenin. Eleven bacterial endophytes were isolated from the bulbs of D. bulbifera, and based on 16S rRNA gene sequencing, were identified as belonging to five genera: Enterobacter, Pseudomonas, Bacillus, Priestia, and Staphylococcus. Subsequently, a preliminary screening of their crude extracts for diosgenin production was performed using Fourier transform infrared spectroscopy (FTIR). The Pseudomonas aeruginosa D3 crude extract was found to have FTIR spectra similar to that of standard diosgenin. The endophyte-mediated diosgenin production was further confirmed by High-Performance Liquid Chromatography (HPLC) and Liquid Chromatography-High Resolution Mass Spectrometry (LC-HRMS) analysis. This is the first report of diosgenin production by a bacterial endophyte of D. bulbifera, and highlights the potential of exploring endophytes as a promising alternative source for plant metabolites.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 6","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147765844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pilosibacter rotomagensis sp. nov., a Butyrate-Producing Bacterium Isolated from Human Faeces.","authors":"Noémie Gloanec, Marion Huré, Laetitia Bailly, Émilie Petit, Corinne Loutelier-Bourhis, Didier Goux, Moïse Coëffier, David Ribet","doi":"10.1007/s00284-026-04906-1","DOIUrl":"https://doi.org/10.1007/s00284-026-04906-1","url":null,"abstract":"","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 6","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147765799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Network Analysis of Wheat and Couchgrass Rhizobacteria Highlights Candidate Biocontrol Agents for Improved Crop Resilience.","authors":"Nataliya Pronovich, Darya Tarasova, Gulnaz Galieva, Polina Galitskaya, Svetlana Selivanovskaya, Polina Kuryntseva","doi":"10.1007/s00284-026-04904-3","DOIUrl":"https://doi.org/10.1007/s00284-026-04904-3","url":null,"abstract":"","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 6","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147765752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Production Temperature on Metarhizium brunneum F52: Growth, Germination, and Virulence for Biocontrol.","authors":"David Sandala, Andrea Bohata, Jana Lencová, Rakshandha Luharch, Veronika Bártová, Jan Bárta","doi":"10.1007/s00284-026-04894-2","DOIUrl":"https://doi.org/10.1007/s00284-026-04894-2","url":null,"abstract":"<p><p>The physiological performance and pathogenicity of the entomopathogenic fungus Metarhizium brunneum F52 were evaluated as a function of both inoculum production and incubation temperatures. This study assessed the impact of five production temperatures (10, 15, 20, 25, and 30 °C) on conidial germination, radial growth, spore production, and virulence against Tenebrio molitor larvae. No conidial germination occurred at 10 °C across all treatments. At a suboptimal incubation temperature of 15 °C, germination was initiated exclusively by conidia produced at 15 °C, suggesting a thermal priming effect. Complete germination (GI ≥ 0.5) was achieved at 20 °C and above for all thermal origins. Radial growth peaked at 20 °C, whereas maximal conidial density was achieved at 25 °C, with a value of 9.19 × 10⁶ spores mm⁻². In contrast, heat-stressed inocula produced at 30 °C exhibited significant reductions in vegetative vigour and reproductive output. Virulence assays demonstrated that all inocula, except for those produced at 30 °C, achieved the mortality threshold (FDI ≥ 1.0) within seven days. The most rapid beginning of sporulation (FDI 2.5) occurred at 25 °C. Inocula produced at 15 °C consistently outperformed heat-stressed origins under fluctuating thermal stress, reaching infection milestones significantly faster. These results indicate that the thermal history of the inoculum primes conidial quality and pathogenicity, underscoring the need to match mass-production temperatures to target field conditions to optimise biocontrol efficacy.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 6","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147765663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cantharellus guyanensis (Basidiomycota, Hydnaceae): A Gourmet Mushroom in the Brazilian Cerrado.","authors":"Robson Bernardo Silveira-Silva, Felipe Wartchow, Lucas Leonardo-Silva, Fábio Neves Vieira, Solange Xavier-Santos","doi":"10.1007/s00284-026-04899-x","DOIUrl":"https://doi.org/10.1007/s00284-026-04899-x","url":null,"abstract":"<p><p>Cantharellus is an important genus of edible fungi with a worldwide distribution, whose species are recognized for their exceptional taste, beauty, and unique texture. Species of Cantharellus are terrestrial ectomycorrhizal fungi valued for their nutritional and medicinal properties, with demonstrated therapeutic potential due to their anti-inflammatory, antimicrobial, and antigenotoxic activities. Cantharellus guyanensis, originally described in 1854 from French Guiana, grows on soil and is currently known from restricted areas in South America, including the Amazon and Atlantic forests in Brazil. Appreciated in gourmet cuisine, C. guyanensis is reported here for the first time in the Cerrado biome. The species was confirmed through morphological and phylogenetic analysis (ITS, LSU and TEF1-α genetic markers), and the voucher is deposited in the fungaria of the Universidade Estadual de Goiás (HUEG-Fungi) and Universidade Federal da Paraíba (JPB). Its nutritional potential determined by bromatological analysis revealed good storage stability, maintaining its high concentration of general digestible nutrients and low lipid contents. This record expands knowledge about the distribution of the genus among Brazilian biomes and reveals an excellent alternative food resource for the Cerrado.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 6","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13110203/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147765698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}