Current Microbiology最新文献

{"title":"Evaluation of the Antimicrobial Activity of Triple Enzyme-Embedded Organic–Inorganic Hybrid Nanoflowers (hNFs) in Comparison with Powerful Antimicrobial Agent Chitosan","authors":"Duygu Aydemir, Seda Çakır, Nalan Özdemir, Nuriye Nuray Ulusu","doi":"10.1007/s00284-024-03884-6","DOIUrl":"https://doi.org/10.1007/s00284-024-03884-6","url":null,"abstract":"<p>Organic<b>–</b>inorganic hybrid nanoflowers (hNFs) have high stability, reusability, low production cost, and overcome substrate/product inhibition. Antimicrobial activity of various hNFs has been reported to overcome environmental microbial contaminations and infections, which are considered major public health problems. α-amylase, protease, and lipase are the most common industrial enzymes exerting antimicrobial activity; therefore, we synthesized triple enzyme (α-amylase, protease, and lipase)-embedded hNFs by using pancreatin to evaluate their antimicrobial activity in comparison with one of the most potent antimicrobial polymer chitosan. The broad spectrum of the antimicrobial properties of chitosan is used in industrial products, including cosmetics, food, agriculture, pharmaceuticals, and textiles. SEM analysis, thermogravimetric analysis (TGA), and the degree of deacetylation (%DD) were performed for chitosan characterization, where SEM, FTIR, EDX, and XRD analyses were performed for the characterization of hNFs. The catalytic activity of pancreatin and hNFs was evaluated by measuring lipase, α-amylase, and protease enzyme activities at 37 °C. Antibacterial activities of hNFs, pancreatin, and chitosan were tested on gram-positive (<i>Staphylococcus aureus)</i> and gram-negative (<i>Escherichia coli</i>) bacteria, compared to the pancreatin and chitosan via agar and broth dilution methods. hNFs showed enhanced catalytic activity for protease, lipase, and α-amylase compared to pancreatin at different pH values (pH 8, 9). hNFs showed catalytic activity after being washed and reused up to six times, indicating their reusability and recoverability. hNFs showed significant antimicrobial activity, such as chitosan, <i>Staphylococcus aureus,</i> and <i>Escherichia coli,</i> compared to pancreatin. Our novel hNFs can be used to develop antimicrobial technologies to fight against environmental microbial contaminations and antibiotic resistance-driven environmental pathogens.</p><h3 data-test=\"abstract-sub-heading\">Graphical Abstract</h3>\u0000","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142262106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Vitro Probiotic Characterization of Yeasts with their Postbiotics' Antioxidant Activity and Biofilm Inhibition Capacity","authors":"Furkan Aydın, Yeşim Aktepe, Halil İbrahim Kahve, İbrahim Çakır","doi":"10.1007/s00284-024-03881-9","DOIUrl":"https://doi.org/10.1007/s00284-024-03881-9","url":null,"abstract":"<p>This study evaluated the in vitro probiotic potential and postbiotic properties of yeast strains isolated from traditional fermented foods, emphasizing antioxidant activity (AOA) and biofilm inhibition capacity (BIC). The yeasts were molecularly confirmed using start codon targeted polymorphisms as <i>Kluyveromyces lactis</i> (<i>n</i> = 17), <i>Saccharomyces cerevisiae</i> (<i>n</i> = 9), <i>Pichia kudriavzevii</i> (<i>n</i> = 6), <i>P. fermentans</i> (<i>n</i> = 4), <i>Wickerhamomyces anomalus</i> (<i>n</i> = 2), and <i>Torulaspora delbrueckii</i> (<i>n</i> = 1). The probiotic assessment of live cells included viability in simulated gastric and pancreatic juices, autoaggregation, hydrophobicity, and AOA, using <i>S</i>. <i>boulardii</i> MYA-796 as reference. Additionally, cell-free supernatants (CFS) were tested for AOA and BIC against <i>Cronobacter</i> <i>sakazakii</i>, <i>Listeria</i> <i>monocytogenes</i>, <i>Pseudomonas</i> <i>aeruginosa</i>, and <i>Staphylococcus aureus</i>. Several strains exhibited significantly higher in vitro probiotic characteristics compared to <i>S</i>. <i>boulardii</i> MYA-796 (<i>P</i> &lt; 0.05), particularly in gastric and pancreatic survival, hydrophobicity, and AOA. Notably, CFS exhibited greater AOA than live cells and strong BIC, especially against <i>L</i>. <i>monocytogenes</i> and <i>S</i>. <i>aureus</i>. Multivariate analysis identified <i>K</i>. <i>lactis</i> TC11, <i>S</i>. <i>cerevisiae</i> M33T1-2, <i>P</i>. <i>kudriavzevii</i> S96, <i>W</i>. <i>anomalus</i> OB7Y1, and <i>T</i>. <i>delbrueckii</i> KY31 as having superior probiotic properties, attributed to enhanced gastric survival, autoaggregation, and AOA. CFS of <i>S</i>. <i>cerevisiae</i> M33T1-2 and <i>T</i>. <i>delbrueckii</i> KY31 demonstrated significant BIC, with over 60% inhibition across all tested pathogens.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142261979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioxidant Activities of Exopolysaccharides Extracts from Two Endemic Fungi from Patagonia","authors":"Ana L. Gallo, Jorge A. Marfetán, María L. Vélez","doi":"10.1007/s00284-024-03883-7","DOIUrl":"https://doi.org/10.1007/s00284-024-03883-7","url":null,"abstract":"<p>A great number of free radicals have a negative impact on the human body, and an increased interest in the identification of new natural molecules with antioxidant properties has emerged due to concerns about synthetic antioxidants. Here, the antioxidant effect of four exo-polysaccharides (EPS) extracts obtained from submerged cultivation of <i>Nothophellinus andinopatagonicus</i> and <i>Pseudoinonotus crustosus</i> (N and P, respectively) in two culture media (M1 and M2) at 2 concentrations (100 and 250 µg/ml) was studied; then, its relation with the chemical composition of the EPS was evaluated. To assess the antioxidant activities of the extracts, several in vitro assays were performed: DPPH and ABTS radical scavenging, ferric-reducing antioxidant power, chelating ability on ferrous ions, and inhibition of the lipid peroxidation. The concentrations tested here were much lower than those reported in previous works. Despite variations in chemical composition and monosaccharide profiles among the extracts, all demonstrated antioxidant activity, although the type of activity differed; only P-M1 exhibited a good antioxidant activity across all assays. This extract contained the highest proportion of phenolic compounds, and also displayed the highest radical scavenging activity. Although the utilization of polysaccharides as functional food ingredients remains limited, we propose P-M1 as a promising candidate for a nutraceutical product. Additionally, a formulation could be made with a combination of extracts to create an antioxidant-rich supplement. Additional research is needed to confirm our findings in a cellular environment and to elucidate the mechanisms that drive their antioxidant activities, ultimately facilitating their development and utilization as nutraceutical products.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142262114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of a Potent Strain of Plant Growth-Promoting Bacteria (PGPB), Bacillus subtilis S1 on Bacterial Community Composition, Enzymatic Activity, and Nitrogen Content in Cucumber Rhizosphere Soils","authors":"Meng-Ting Zhang, Yu-Qing Bao, Bao-Yun Feng, Lu-Rong Xu, Ya-Ting Zhang, Er-Xing Wang, Yun-Peng Chen","doi":"10.1007/s00284-024-03876-6","DOIUrl":"https://doi.org/10.1007/s00284-024-03876-6","url":null,"abstract":"<p>Antagonistic bacterial strains from <i>Bacillus</i> spp. have been widely studied and utilized in the biocontrol of phytopathogens and the promotion of plant growth, but their impacts on the rhizosphere microecology when applied to crop plants are unclear. Herein, the effects of applying the antagonistic bacterium <i>Bacillus subtilis</i> S1 as a biofertilizer on the rhizosphere microecology of cucumbers were investigated. In a pot experiment on cucumber seedlings inoculated with S1, 3124 bacterial operational taxonomic units (OTUs) were obtained from the rhizosphere soils using high-throughput sequencing of 16S rRNA gene amplicons, and the most abundant phylum was <i>Proteobacteria</i> that accounted for 49.48% in the bacterial community. S1 treatment significantly reduced the abundances of soil bacterial taxa during a period of approximately 30 days but did not affect bacterial diversity in the rhizosphere soils of cucumbers. The enzymatic activities of soil nitrite reductase (S-Nir) and dehydrogenase (S-DHA) were significantly increased after S1 fertilization. However, the activities of soil urease (S-UE), cellulase (S-CL), and sucrase (S-SC) were significantly reduced compared to the control group. Additionally, the ammonium- and nitrate-nitrogen contents of S1-treated soil samples were significantly lower than those of the control group. S1 fertilization reshaped the rhizosphere soil bacterial community of cucumber plants. The S-CL activity and nitrate-nitrogen content in rhizosphere soil affected by S1 inoculation play important roles in altering the abundance of rhizosphere soil microbiota.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142261983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of Bacillus pacificus KUMBNGBT-39-Derived Polyhydroxybutyrate and Impact of Physicochemical Factors on its Production","authors":"G. Nandish, S. Akarsh, D. Manjunatha, H. V. Sowmya, B. Thippeswamy","doi":"10.1007/s00284-024-03879-3","DOIUrl":"https://doi.org/10.1007/s00284-024-03879-3","url":null,"abstract":"<p>The extensive use of various chemicals in synthetic plastics is toxic and threatens the biosphere. To address this, the study aimed to isolate, screen, characterize, optimize, and quantify polyhydroxybutyrate (PHB)-producing bacteria using cost-effective residues. Isolated from a landfill site, the Gram-positive, rod-shaped, spore-forming, motile bacterium with intracellular PHB granules was identified as <i>Bacillus pacificus</i> based on phenotypic and genotypic characteristics. Optimal PHB production parameters included a nutrient broth medium, 72 h of incubation, a temperature of 37° C, a pH of 7.0, glucose as the carbon source, ammonium chloride as the nitrogen source, and a carbon-to-nitrogen ratio of 4:1, resulting in a 1.42-fold PHB production increase. <i>B. pacificus</i> was also cultured on various low-cost substrates. Among the oil wastes, feedstock showed the highest PHB production (1.983 ± 0.005 g/L) and among agricultural residues, the maximum PHB was obtained from rice bran (1.626 ± 0.01 g/L). UV–visible spectrophotometric, FT-IR, and HR-LCMS analysis of extracted PHB confirmed characteristics of PHB molecules (ʎ-max at 210 nm, functional groups between 1152 and 2925 cm⁻¹). The ¹H NMR analysis revealed distinct signals for protons resonating at aliphatic CH₃ proton groups, bridged CH protons, and shielding CH₂ proton regions that matched PHBs. Thermal gravimetric analysis (TGA) and direct scanning colorimetric (DSC) analysis revealed 89.4% degradation and melting temperature at 124.1 °C for the extracted PHB compound.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142261982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Complete Genome of Achromobacter xylosoxidans, a Nitrogen-Fixing Bacteria from the Rhizosphere of Cowpea (Vigna unguiculata [L.] Walp) Tolerant to Cucumber Mosaic Virus Infection","authors":"Adedapo O. Adediji, James A. Ojo, Adebayo A. Olowoake, Khadijat O. Alabi, Gabriel I. Atiri","doi":"10.1007/s00284-024-03882-8","DOIUrl":"https://doi.org/10.1007/s00284-024-03882-8","url":null,"abstract":"<p><i>Achromobacter xylosoxidans</i> is one of the nitrogen-fixing bacteria associated with cowpea rhizosphere across Africa. Although its role in improving soil fertility and inducing systemic resistance in plants against pathogens has been documented, there is limited information on its complete genomic characteristics from cowpea roots. Here, we report the complete genome sequence of <i>A. xylosoxidans</i> strain DDA01 isolated from the topsoil of a field where cowpea plants tolerant to cucumber mosaic virus (CMV) were grown in Ibadan, Nigeria. The genome of DDA01 was sequenced via Illumina MiSeq and contained 6,930,067 nucleotides with 67.55% G + C content, 73 RNAs, 59 tRNAs, and 6421 protein-coding genes, including those associated with nitrogen fixation, phosphate solubilization, Indole3-acetic acid production, and siderophore activity. Eleven genetic clusters for secondary metabolites, including alcaligin, were identified. The potential of DDA01 as a plant growth-promoting bacteria with genetic capabilities to enhance soil fertility for resilience against CMV infection in cowpea is discussed. To our knowledge, this is the first complete genome of diazotrophic bacteria obtained from cowpea rhizosphere in sub-Saharan Africa, with potential implications for improved soil fertility, plant disease resistance, and food security.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142262111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reactive Oxygen Species Mediate the Bactericidal Activity of Chlorine Against Salmonella","authors":"Mohammed Aljuwayd, Israa Abdullah Malli, Steven C. Ricke, Young Min Kwon","doi":"10.1007/s00284-024-03880-w","DOIUrl":"https://doi.org/10.1007/s00284-024-03880-w","url":null,"abstract":"<p>Chlorine and its derivatives have been used as an antibacterial agent to reduce <i>Salmonella</i> contamination in poultry meat during processing. We evaluated the survival of 4 different <i>Salmonella</i> serotypes (Typhimurium, Enteritidis, Heidelberg, and Gaminara) in the presence of 50 ppm sodium hypochlorite (NaOCl) alone or with the addition of thiourea (radical scavenger) or Dip (iron chelator) to determine the contribution of reactive oxygen species (ROS) in the bactericidal activity of NaOCl. The result showed that for all four serotypes the addition of thiourea or Dip significantly increased the % survival as compared to the respective NaOCl treatment groups, while it was significantly higher with thiourea as compared to Dip (<i>P</i> &lt; 0.05). We also evaluated the survival of 11 deletion mutants of <i>S.</i> Typhimurium, which were demonstrated to increase (∆<i>atpC</i>, ∆<i>cyoA</i>, ∆<i>gnd</i>, ∆<i>nuoG</i>, ∆<i>pta</i>, ∆<i>sdhC,</i> and ∆<i>zwf</i>) or decrease the production of ROS (∆<i>edd</i>, ∆<i>fumB</i>, ∆<i>pykA,</i> and ∆<i>tktB</i>) in <i>Escherichia coli</i> (<i>E. coli</i>), in the presence of 50 ppm. The results showed that only two (∆<i>sdhC</i> and ∆<i>zwf</i>) out of 7 ROS-increasing mutants showed reduced % survival as compared to the wild-type (<i>P</i> &lt; 0.05), while all four deletion ROS-decreasing mutants showed significantly higher % survival as compared to the wild-type (<i>P</i> &lt; 0.05). This work suggests that the production of ROS is a major component of the bactericidal activity of NaOCl against <i>Salmonella</i> serotypes and there might be a significant difference in the metabolic pathways involved in ROS production between <i>Salmonella</i> and <i>E. coli</i>.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142262110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From Skin to Gut: Understanding Microbial Diversity in Rana amurensis and R. dybowskii","authors":"Ming-he Yang, Hong Liu, Hao Wang, Zhao-ning Lu, Xiao-yun Han, Zhi-wen Luo, Li-gang Wu, Qing Tong","doi":"10.1007/s00284-024-03868-6","DOIUrl":"https://doi.org/10.1007/s00284-024-03868-6","url":null,"abstract":"<p>Amphibians face the threat of decline and extinction, and their health is crucially affected by the microbiota. Their health and ecological adaptability essentially depend on the diverse microbial communities that are shaped by unique host traits and environmental factors. However, there is still limited research on this topic. In this study, cutaneous (C) and gut (G) microbiota in <i>Rana amurensis</i> (A) and <i>R. dybowskii</i> (D) was analyzed through 16S amplicon sequencing. Groups AC and DC significantly differed in alpha diversity, while the gut groups (AG and DG) showed no such differences. Analyses of Bray–Curtis dissimilarity matrix and unweighted UniFrac distances showed significant differences in cutaneous microbiota between groups AC and DC, but not between groups AG and DG. Stochastic processes significantly influenced the assembly of cutaneous and gut microbiota in amphibians, with a notably higher species dispersal rate in the gut. The predominant phyla in the skin of <i>R. amurensis</i> and <i>R. dybowskii</i> were Bacteroidetes and Proteobacteria, respectively, with significant variations in Bacteroidota. Contrarily, the gut microbiota of both species was dominated by Firmicutes, Proteobacteria, and Bacteroidetes, without significant phylum-level differences. Linear discriminant analysis effect size (LEfSe) analysis identified distinct microbial enrichment in each group. Predictive analysis using phylogenetic investigation of communities by reconstruction of unobserved states 2 (PICRUSt2) revealed the significant functional pathways associated with the microbiota, which indicates their potential roles in immune system function, development, regeneration, and response to infectious diseases. This research underscores the critical impact of both host and environmental factors in shaping amphibian microbial ecosystems and emphasizes the need for further studies to explore these complex interactions for conservation efforts.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142213843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioactivity of Amphidinol-Containing Extracts of Amphidinium carterae Grown Under Varying Cultivation Conditions","authors":"Maria Elena Barone, Elliot Murphy, David Fierli, Floriana Campanile, Gerard T. A. Fleming, Olivier P. Thomas, Nicolas Touzet","doi":"10.1007/s00284-024-03862-y","DOIUrl":"https://doi.org/10.1007/s00284-024-03862-y","url":null,"abstract":"<p>Microalgae are of great interest due to their ability to produce valuable compounds, such as pigments, omega-3 fatty acids, antioxidants, and antimicrobials. The dinoflagellate genus <i>Amphidinium</i> is particularly notable for its amphidinol-like compounds, which exhibit antibacterial and antifungal properties. This study utilized a two-stage cultivation method to grow <i>Amphidinium carterae</i> CCAP 1102/8 under varying conditions, such as blue LED light, increased salinity, and the addition of sodium carbonate or hydrogen peroxide. After cultivation, the biomass was extracted and fractionated using solid-phase extraction, yielding six fractions per treatment. These fractions were analyzed using Liquid Chromatography—High-Resolution Mass Spectrometry (LC-HRMS/MS) to identify their chemical components. Key amphidinol compounds (AM-B, AM-C, AM-22, and AM-A) were identified, with AM-B being the most abundant in Fraction 4, followed by AM-C. Fraction 5 also contained a significant amount of AM-C along with an unknown compound. Fraction 4 returned the highest antimicrobial activity against the pathogens <i>Staphylococcus aureus</i>, <i>Enterococcus faecalis,</i> and <i>Candida albicans</i>, with Minimal Biocidal Concentrations (MBCs) ranging from 1 to 512 µg/mL. Results indicate that the modulation of both amphidinol profile and fraction bioactivity can be induced by adjusting the cultivation parameters used to grow two-stage batch cultures of <i>A. carterae</i>.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142226950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic Role of Secondary Metabolites from Probiotic Strains for Ehrlich Solid Tumors in Mice","authors":"Neima K. Al-Senosy, Noura El-Kattan, Enas A. Hassan, Hemmat M. Abd-Elhady, Abdelkader Hazem, Mona A. Ashour, Mosaad A. Abdel-Wahhab","doi":"10.1007/s00284-024-03864-w","DOIUrl":"https://doi.org/10.1007/s00284-024-03864-w","url":null,"abstract":"<p>This study aimed to screen the bioactive components in <i>Streptococcus equinus</i> WC₁ (SE-WC₁) and <i>Limosilactobacillus reuteri</i> GM₄ (LR-GM₄) and estimate the therapeutic role in Ehrlich solid tumors (EST) mice model. Forty-four male albino EST mice were assigned into 7 groups and treated daily for 2 weeks, including the EST group, the EST mice that received SE-WC₁ at a low or a high dose (0.5 ml *10⁶ or 0.5 ml *10⁸ cfu), the EST mice that received LR-GM₄ at the low or the high dose (0.5 ml *10⁶ or 0.5 ml *10⁸ cfu), and the EST mice that received SE-WC₁ plus LR-GM₄ at the low or the high dose. Tumors were harvested, weighed, examined, and used for the determination of apoptosis-related gene expression. Samples of the intestine, liver, and kidney were gathered for histological examination. The GC–MS identified 24 and 36 bioactive compounds in SE-WC₁ and LR-GM₄, respectively. The main compound in SE-WC₁ was lupeol; however, the main compound in LR-GM₄ was retinaldehyde. EST mice showed disturbances in Bcl-2, Bax, and p53 mRNA expression along with histological changes in the intestine, liver, and kidney. Administration of both bacterial strains reduced the tumor weight, alleviated the disturbances in the gene expression, and improved the histological structure of the intestine, liver, and kidney in a dose-dependent. Moreover, LR-GM₄ was more effective than SE-WC₁ due to its higher content of bioactive compounds. It could be concluded that these strains of probiotics are promising for the treatment of solid tumors.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142213844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}