Current Microbiology最新文献_第10页

Immersion-Based Candida albicans Infection in Adult Zebrafish Induces Distinct Behavioral, Immune and Oxidative Responses in a Dose- and Time-Dependent Manner. 成年斑马鱼浸泡白色念珠菌感染诱导不同的行为，免疫和氧化反应，剂量和时间依赖的方式。

IF 2.8 3区生物学

Current Microbiology Pub Date : 2026-08-26 DOI: 10.1007/s00284-026-05109-4

Marcos Otávio Bueno, Ricardo Cervini, Vinicius Cataldo Ferraz, Adriano Tony Ramos, Claudriana Locatelli, Ariana Centa, Gustavo Colombo Dal-Pont, João Paulo Assolini

{"title":"Immersion-Based Candida albicans Infection in Adult Zebrafish Induces Distinct Behavioral, Immune and Oxidative Responses in a Dose- and Time-Dependent Manner.","authors":"Marcos Otávio Bueno, Ricardo Cervini, Vinicius Cataldo Ferraz, Adriano Tony Ramos, Claudriana Locatelli, Ariana Centa, Gustavo Colombo Dal-Pont, João Paulo Assolini","doi":"10.1007/s00284-026-05109-4","DOIUrl":"10.1007/s00284-026-05109-4","url":null,"abstract":"Candidiasis caused by Candida albicans represents a major opportunistic infection, particularly in hospitalized individuals, with high morbidity and mortality rates. Experimental models that reproduce natural routes of exposure and allow integrated analyses of host responses remain limited. In this study, we established and characterized an immersion-based infection model of C. albicans in adult zebrafish (Danio rerio) to investigate systemic, behavioral, immunological, oxidative, and histopathological outcomes. Adult zebrafish were exposed by immersion to different fungal concentrations (1 × 10⁶ or 1 × 10⁸ CFU/mL) for 10-24 h. Fungal burden, exploratory behavior, antioxidant capacity (ABTS), TNF-α levels, and tissue alterations were subsequently assessed. Exposure to 1 × 10⁸ CFU/mL for 24 h resulted in a significant increase in fungal burden without inducing mortality, characterizing a sublethal infection. Infection modulated behavioral parameters, tissue-specific alterations in antioxidant activity and a marked reduction of TNF-α levels in the liver and brain were observed, suggesting disruption of immune-redox homeostasis. Histopathological analyses revealed mild inflammatory responses and increased melanomacrophage accumulation, particularly in skin and gills tissues. Overall, this study demonstrates that adult zebrafish immersion constitutes a biologically relevant and translational model for investigating candidiasis, enabling integrated evaluation of host-pathogen interactions and systemic responses under environmentally relevant conditions.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 10","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13518388/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148826253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nitrogen Source Screening on Agar Medium Followed by Submerged Cultivation of Pleurotus albidus Mycelium for Nutritional and Amino Acid Characterization. 琼脂培养基氮源筛选及平菇菌丝深层培养营养及氨基酸特性研究。

IF 2.8 3区生物学

Current Microbiology Pub Date : 2026-08-26 DOI: 10.1007/s00284-026-05119-2

Eduarda Monteiro Martins Leitão, Acácio A F Zielinski, Patrícia Poletto

{"title":"Nitrogen Source Screening on Agar Medium Followed by Submerged Cultivation of Pleurotus albidus Mycelium for Nutritional and Amino Acid Characterization.","authors":"Eduarda Monteiro Martins Leitão, Acácio A F Zielinski, Patrícia Poletto","doi":"10.1007/s00284-026-05119-2","DOIUrl":"10.1007/s00284-026-05119-2","url":null,"abstract":"The search for sustainable protein sources has increased interest in filamentous fungi, however, most studies focus on a limited number of established species. Pleurotus albidus, a basidiomycete native to South America, remains little explored for food applications, particularly regarding the nutritional characterization of its mycelium. In this study, P. albidus was initially cultivated on PDA agar supplemented with yeast extract (YE) or ammonium sulfate (AS) at 0.5-2 gN/L for nitrogen source screening. On agar medium, YE reduced the lag phase and accelerated plate colonization, reaching full plate coverage in 8-9 days compared to 11-12 days for AS. Based on these results, the selected condition was subsequently applied to submerged cultivation in which the mycelium grew as spherical pellets and reached 12.4 g/L after 12 days, with 23.8% protein, 26% fiber, and 39% carbohydrates. Chemical score analysis indicated that most essential amino acids (EAAs) exceeded recommended levels; however, sulfur-containing amino acids (methionine + cystine) were limiting (0.6). Threonine showed the highest score (2.3), while lysine (1.2) and tryptophan (1.1) also scored above 1.0. Non-essential amino acids such as glutamic (145 mg/g) and aspartic acid (102 mg/g) predominated. These results highlight the nutritional potential of P. albidus mycelium as a novel protein source and support its further investigation for future food applications, while additional studies on protein digestibility and cultivation optimization are needed to further improve amino acid balance and protein quality.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 10","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13518488/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148826328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Wallemia orionensis sp. nov., a Xerophilic and Halotolerant Fungus Isolated from Air Environment. 从空气环境中分离的一种嗜干耐盐真菌orionensis sp. nov.。

IF 2.8 3区生物学

Current Microbiology Pub Date : 2026-08-24 DOI: 10.1007/s00284-026-05110-x

Jingjing Zhang, Feirong Bai, Tianci Zhang, Tuhuan Wu, Wenhao Wu, Chengshan Cai, Ziying Ma, Yunzhen Liu, Lu Wang, Bangyi Wang, Juan Chen, Su Yao

{"title":"Wallemia orionensis sp. nov., a Xerophilic and Halotolerant Fungus Isolated from Air Environment.","authors":"Jingjing Zhang, Feirong Bai, Tianci Zhang, Tuhuan Wu, Wenhao Wu, Chengshan Cai, Ziying Ma, Yunzhen Liu, Lu Wang, Bangyi Wang, Juan Chen, Su Yao","doi":"10.1007/s00284-026-05110-x","DOIUrl":"10.1007/s00284-026-05110-x","url":null,"abstract":"A novel xerophilic fungus, Wallemia orionensis sp. nov., was isolated from air samples collected in a cake production environment and is described here based on a polyphasic approach, including molecular phylogenetics, morphology, and physiological traits. Phylogenetic analyses using concatenated ITS and SSU rDNA sequences revealed that the three strains form a distinct and well-supported clade within the genus Wallemia, clearly separated from all currently recognized species. Colonies of W. orionensis exhibit slow to moderate growth on media supplemented with high concentrations of glucose or sodium chloride, but fail to grow on media lacking such solutes, confirming its obligate xerophilic nature. The species demonstrates remarkable halotolerance and osmophily, growing optimally in media containing 50% glucose or 10-17% NaCl. Microscopic examination reveals that it exhibits the characteristic morphological features of the genus Wallemia, with arthroconidia formed by the fragmentation of hyphae, appearing as short cylindrical, barrel-shaped, or bluntly rounded structures. The isolation of W. orionensis from air samples in a cake production environment expands the known diversity of the genus Wallemia and contributes to a better understanding of fungal adaptation to high-solute, low-water-activity conditions.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 10","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148812104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Safety Assessment of Lacticaseibacillus rhamnosus OF44, a Previously Isolated Human-origin Probiotic Candidate. 鼠李糖乳杆菌OF44的安全性评价——一种先前分离的人类来源的候选益生菌。

IF 2.8 3区生物学

Current Microbiology Pub Date : 2026-08-23 DOI: 10.1007/s00284-026-05130-7

Fangping Yang, Lei Zhang, Pu Yang, Yongping Xin

{"title":"Safety Assessment of Lacticaseibacillus rhamnosus OF44, a Previously Isolated Human-origin Probiotic Candidate.","authors":"Fangping Yang, Lei Zhang, Pu Yang, Yongping Xin","doi":"10.1007/s00284-026-05130-7","DOIUrl":"10.1007/s00284-026-05130-7","url":null,"abstract":"Lacticaseibacillus rhamnosus is one of the most extensively studied and well-characterized probiotic species. The strain L. rhamnosus OF44, originally isolated from a healthy female fecal sample, has shown particular promise for its ability to modulate the vaginal microenvironment through pH reduction and inflammation control. However, its comprehensive safety profile remains unclear. In this study, we systematically evaluated the safety of the previously identified strain L. rhamnosus OF44 through a series of standardized assessments. Genomic analysis (99.9% coverage) revealed the absence of virulence genes, while identifying four genes associated with resistance to chlorhexidine and copper ions; these have been demonstrated to be intrinsic to L. rhamnosus. Additionally, phenotypic analysis, including minimum inhibitory concentration (MIC) testing, reverse mutation testing, β-hemolysis assessment, biological amines and D-lactate production (0.571 ± 0.005 mM), and genetic stability testing, revealed no adverse traits. Finally, in vivo studies (acute oral toxicity at up to 3 × 10¹² CFU/kg, with LD₅₀ > 15.0 g/kg·BW) confirmed that L. rhamnosus OF44 exhibited no detectable acute oral toxicity and pathogenicity. These results indicate that L. rhamnosus OF44 has a favorable safety profile under the tested conditions, although further chronic toxicity and human studies are warranted.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 10","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148808513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic Characterization of a Multidrug-Resistant Serratia marcescens Isolate Recovered from a Neonatal Meningitis Case in Iraq. 从伊拉克新生儿脑膜炎病例中分离出的耐多药粘质沙雷菌的基因组特征

IF 2.8 3区生物学

Current Microbiology Pub Date : 2026-08-22 DOI: 10.1007/s00284-026-05127-2

Husam Al-Hraishawi, Namariq Al-Saadi, Shilan Jabbar, Mushtaq Al-Rubaye

{"title":"Genomic Characterization of a Multidrug-Resistant Serratia marcescens Isolate Recovered from a Neonatal Meningitis Case in Iraq.","authors":"Husam Al-Hraishawi, Namariq Al-Saadi, Shilan Jabbar, Mushtaq Al-Rubaye","doi":"10.1007/s00284-026-05127-2","DOIUrl":"10.1007/s00284-026-05127-2","url":null,"abstract":"Serratia marcescens (S. marcescens) is an opportunistic pathogen associated with severe hospital-acquired infections, particularly in neonatal intensive care units. Here, we report the first whole-genome sequencing and characterization of a multidrug-resistant S. marcescens isolate (NOA1) recovered from the cerebrospinal fluid of a neonate with acute meningitis in Iraq. The isolate was identified using the VITEK® 2 system and confirmed by 16 S rRNA gene sequencing. Antimicrobial susceptibility testing demonstrated a multidrug-resistant phenotype, with resistance to β-lactams, aminoglycosides, carbapenems, nitrofurantoin, and trimethoprim/sulfamethoxazole, while susceptibility to ciprofloxacin, levofloxacin, and tigecycline was retained. Whole-genome sequencing produced a 5.29 Mb draft genome with 59.67% GC content, an N50 of 1.35 Mb, and an average sequencing coverage of 200x. Genome analysis identified 15 antimicrobial resistance genes, including 10 located on plasmid-associated contigs, suggesting a potential role of mobile genetic elements in resistance dissemination. Comparative genomic analysis revealed 13,971 sequence variants relative to the closest available reference genome, indicating substantial genetic divergence. Phylogenetic analysis placed NOA1 within a distinct lineage of clinical S. marcescens isolates. This study highlights the utility of whole-genome sequencing for comprehensive characterization of multidrug-resistant S. marcescens and underscores the importance of integrating phenotypic and genomic approaches for accurate antimicrobial resistance profiling.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 10","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148789638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential Roles of Ag and ZnO in the Bactericidal Activity of Ag/ZnO-Hydroxyapatite Nanocomposites against Multidrug-Associated Methicillin-Resistant Staphylococcus aureus. Ag和ZnO在Ag/ZnO-羟基磷灰石纳米复合材料对多药相关耐甲氧西林金黄色葡萄球菌杀菌活性中的差异作用

IF 2.8 3区生物学

Current Microbiology Pub Date : 2026-08-22 DOI: 10.1007/s00284-026-05128-1

Dong H Kwon, Azzedine Bensalem, Sophia Fu

{"title":"Differential Roles of Ag and ZnO in the Bactericidal Activity of Ag/ZnO-Hydroxyapatite Nanocomposites against Multidrug-Associated Methicillin-Resistant Staphylococcus aureus.","authors":"Dong H Kwon, Azzedine Bensalem, Sophia Fu","doi":"10.1007/s00284-026-05128-1","DOIUrl":"10.1007/s00284-026-05128-1","url":null,"abstract":"Methicillin-resistant Staphylococcus aureus (MRSA) is often resistant to multiple antibiotic classes and extremely difficult to treat, highlighting the urgent need for new drugs or alternative strategies. Hydroxyapatite (HAp) combined with silver (Ag) and zinc oxide (ZnO) to form an Ag/ZnO-HAp nanocomposite has been widely studied for its antibacterial properties. This nanocomposite is often assumed to produce additive or synergistic antibacterial effects. Four Ag/ZnO-HAp nanocomposites with different Ag-to-ZnO mole ratios: nanocomposite-A (0.005:0.005), nanocomposite-B (0.01:0.005), nanocomposite-C (0.005:0.01), and nanocomposite-D (0.01:0.01) were used to understand the roles of Ag and ZnO in antibacterial activity against MRSA ATCC700699. Agar diffusion tests of nanocomposites A, B, C, and D produced inhibition zones of 8, 9, 6, and 8 mm, respectively, whereas unmodified HAp showed no inhibition zone. Bactericidal assays revealed log reductions of 5.27, 7.16, 5.00, and 5.81 for nanocomposites A, B, C, and D at 0.0625 mg/mL over 18 h, respectively. Specific death rates (min-1) for nanocomposites A, B, C, and D over 240 min were 0.062, 0.075, 0.058, and 0.064; D-values (minutes) were 16.1, 13.3, 17.2, and 15.6, respectively. Minimum bactericidal concentrations for 9 MRSA and 1 methicillin-sensitive S. aureus clinical isolates ranged from ≤ 0.06 to > 2 mg/mL, regardless of antibiotic susceptibility profiles. Overall, the nanocomposites exhibit bactericidal activity in the order B > D> A > C. Ag content primarily drives bactericidal activity, whereas ZnO reduces Ag's bactericidal effectiveness. These findings suggest that optimizing the Ag-to-ZnO ratio is crucial for maximizing bactericidal activity. Additionally, customizing the nanocomposites to clinical isolates may be essential to maximize biomedical applications.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 10","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148789686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of 4-Amino-7-chloroquinoline Derivative with In Vitro Activity Against Chikungunya Virus. 具有抗基孔肯雅病毒活性的4-氨基-7-氯喹啉衍生物的鉴定。

IF 2.8 3区生物学

Current Microbiology Pub Date : 2026-08-22 DOI: 10.1007/s00284-026-05133-4

Adriana Cotta Cardoso Reis, Camila Portruneli, Lívia da Cunha Agostini, Camila Rezende Minelli, Luana Neves Dias, Adalberto José de Lima, Breno de Mello Silva, Cíntia Lopes de Brito Magalhães, Glenda Nicioli da Silva, Guilherme Rocha Pereira, Geraldo Célio Brandão

{"title":"Identification of 4-Amino-7-chloroquinoline Derivative with In Vitro Activity Against Chikungunya Virus.","authors":"Adriana Cotta Cardoso Reis, Camila Portruneli, Lívia da Cunha Agostini, Camila Rezende Minelli, Luana Neves Dias, Adalberto José de Lima, Breno de Mello Silva, Cíntia Lopes de Brito Magalhães, Glenda Nicioli da Silva, Guilherme Rocha Pereira, Geraldo Célio Brandão","doi":"10.1007/s00284-026-05133-4","DOIUrl":"10.1007/s00284-026-05133-4","url":null,"abstract":"Arboviruses such as chikungunya virus (CHIKV), Zika virus (ZIKV), and Mayaro virus (MAYV) represent significant global health challenges due to the lack of effective antiviral therapies. Quinoline-based compounds have emerged as promising scaffolds for antiviral drug development. In this study, five 4-amino-7-chloroquinoline derivatives were synthesized and evaluated for their in vitro antiviral activity against CHIKV, ZIKV, and MAYV in Vero cells. Cytotoxicity and antiviral effects were assessed using a tetrazolium-based assay, followed by complementary analyses including cytopathic effect inhibition, virucidal assay, and viral load quantification by RT-qPCR. Among the tested compounds, derivative 6 demonstrated the most pronounced anti-CHIKV activity, with a low micromolar effective concentration and moderate selectivity index. This compound significantly reduced viral replication, preserved cell monolayer integrity, and decreased viral titers, achieving substantial viral load reduction confirmed by RT-qPCR. No virucidal activity was observed. In contrast, compound 6 inhibited the virus-induced cytopathic effect when administered after viral adsorption, suggesting that its antiviral activity is unlikely to result from interference with viral attachment or adsorption but rather from inhibition of a post-adsorption stage of the viral replication cycle. Overall, these findings highlight 4-amino-7-chloroquinoline derivatives as a promising class of antiviral agents and identify compound 6 as a potential lead candidate for further development against CHIKV.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 10","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13499868/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148788808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Untargeted Metabolomics and Bioactivity Screening of Mycelial Culture Extracts from Marasmioid Fungi. 类麻瓜真菌菌丝培养提取物的非靶向代谢组学和生物活性筛选。

IF 2.8 3区生物学

Current Microbiology Pub Date : 2026-08-21 DOI: 10.1007/s00284-026-05131-6

Carol Alexis Olivares-García, Roberto Gamboa-Becerra, Leonardo Serrano-Márquez, Juan L Monribot-Villanueva, José A Guerrero-Analco, Guillermo Mendoza, Leticia Montoya, Víctor M Bandala, Antero Ramos

{"title":"Untargeted Metabolomics and Bioactivity Screening of Mycelial Culture Extracts from Marasmioid Fungi.","authors":"Carol Alexis Olivares-García, Roberto Gamboa-Becerra, Leonardo Serrano-Márquez, Juan L Monribot-Villanueva, José A Guerrero-Analco, Guillermo Mendoza, Leticia Montoya, Víctor M Bandala, Antero Ramos","doi":"10.1007/s00284-026-05131-6","DOIUrl":"10.1007/s00284-026-05131-6","url":null,"abstract":"Basidiomycota is a cosmopolitan fungal phylum recognized for its nutritional properties and as a natural source of bioactive compounds. Within this group, the family Marasmiaceae Roze ex Kühner remains comparatively understudied. In this study, we performed a metabolic characterization and bioactivity screening of four marasmioid fungal strains using untargeted metabolomic and bioinformatic tools to analyze the metabolic profiles of broth extracts from strains Montiel04, FGR1289, FGR1276, and CC45, detecting 296, 447, 167, and 99 spectrometric features (m/z_RT), respectively. The extracts exhibited notable antibacterial activity, particularly against Clavibacter michiganensis and Erwinia persicina, with strain FGR1289 demonstrating the highest efficacy. Furthermore, extracts from strains FGR1289 and FGR1276 exhibited significant antioxidant capacity against ABTS+, DPPH, and galvinoxyl radicals. Pathway enrichment analysis identified putative metabolic pathways involved in organic acid biosynthesis, amino acid metabolism, and butyrate, which may contribute to the observed bioactivities. These findings highlight the marasmioid group as a promising reservoir of bioactive metabolites and support further research on their potential pharmaceutical and agricultural applications.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 10","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148788895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterizing Lactic Acid Bacteria in Artisanal Cheeses: Analytical Approaches and Strategic Implications. 表征手工奶酪中的乳酸菌：分析方法和战略意义。

IF 2.8 3区生物学

Current Microbiology Pub Date : 2026-08-21 DOI: 10.1007/s00284-026-05116-5

Amina Bouchemal, Zeyneb Ladouali, Mohammed Benhoula, Fahima Mechiah, Ahlam Inouri, Zoubeida Meghlaoui, Nassim Madi

{"title":"Characterizing Lactic Acid Bacteria in Artisanal Cheeses: Analytical Approaches and Strategic Implications.","authors":"Amina Bouchemal, Zeyneb Ladouali, Mohammed Benhoula, Fahima Mechiah, Ahlam Inouri, Zoubeida Meghlaoui, Nassim Madi","doi":"10.1007/s00284-026-05116-5","DOIUrl":"10.1007/s00284-026-05116-5","url":null,"abstract":"There is growing interest in the identification and characterization of beneficial microbial strains involved in fermented foods. Lactic acid bacteria (LAB) are among the most extensively studied microorganisms due to their ubiquity and functional diversity. In artisanal cheese making, LAB play a central role in ensuring product safety, shaping sensory attributes and defining typicity. This review critically synthesizes the methodological approaches used for LAB characterization ranging from conventional phenotypic assays to advanced molecular and omics-based techniques. It compares their taxonomic resolution, functional insights and operational constraints, drawing on illustrative studies from artisanal cheese systems. Overall, this review highlights how LAB characterization not only contributes to product quality and consistency, but also supports the preservation of microbial biodiversity, enhances the sustainability of artisanal cheese production and strengthens local value chains. Furthermore, it provides a structured framework to guide the selection of appropriate characterization methods according to specific research.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 10","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148789623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of the Alkyl Hydroperoxide Reductase Subunit C1 Gene in Vibrio parahaemolyticus Against Hydrogen Peroxide. 烷基过氧化氢还原酶亚基C1基因在副溶血性弧菌抗过氧化氢中的作用。

IF 2.8 3区生物学

Current Microbiology Pub Date : 2026-08-20 DOI: 10.1007/s00284-026-05134-3

Cheng-Lun Chien, Wen-Chin Chiu, Paula Hsu, Hin-Chung Wong, Chung-Tao Tang

{"title":"Role of the Alkyl Hydroperoxide Reductase Subunit C1 Gene in Vibrio parahaemolyticus Against Hydrogen Peroxide.","authors":"Cheng-Lun Chien, Wen-Chin Chiu, Paula Hsu, Hin-Chung Wong, Chung-Tao Tang","doi":"10.1007/s00284-026-05134-3","DOIUrl":"10.1007/s00284-026-05134-3","url":null,"abstract":"Vibrio parahaemolyticus is a major marine foodborne pathogen and has been a global public health concern since the emergence of pandemic strains in 1996. The catalase KatE1 and alkyl hydroperoxide reductase subunit C1 (AhpC1) are important components of the oxidative stress defense system in this bacterium. In this study, we investigated the role of AhpC1 in protection against H₂O₂-induced oxidative stress using the ΔkatE1, ΔahpC1, ΔkatE1ΔkatE2, and ΔkatE1ΔahpC1 mutant strains of V. parahaemolyticus. Under H2O2 exposure, the ΔkatE1ΔkatE2 mutant showed the highest sensitivity, whereas the ΔkatE1ΔahpC1 mutant exhibited intermediate sensitivity relative to the wild-type strain. Similarly, intracellular reactive oxygen species accumulation was highest in the ΔkatE1ΔkatE2 mutant and moderately increased in the ΔkatE1ΔahpC1 mutant. The ΔkatE1ΔahpC1 mutant also retained a higher level of residual extracellular H₂O₂, which was significantly reduced by complementation with the ahpC1. In addition, ahpC1 expression was detected under normal conditions and appeared to increase following H2O2 treatment. In summary, these findings suggest that AhpC1 functions alongside catalase systems in the oxidative stress defense network of V. parahaemolyticus.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 10","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148788919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0