Current Microbiology最新文献

{"title":"Bacillus sp. Strain Fo03, a Phosphate Solubilizing Bacterial Strain, Promotes Potato Growth and Decrease Inorganic Fertilizer.","authors":"Denisse M de la Paz-Osorio, Nadia de la Portilla-Lopez, Rocío Vaca-Paulín, Jorge Lugo-de la Fuente, Arnoldo Wong-Villarreal, Ivana F Della Mónica, Pedro Del Águila-Juárez, Martha E Mora-Herrera, Gustavo Yañez-Ocampo","doi":"10.1007/s00284-025-04198-x","DOIUrl":"10.1007/s00284-025-04198-x","url":null,"abstract":"<p><p>Biofertilizers are a sustainable biotechnological alternative for decreasing use of inorganic fertilizer. Some bacterial strains, such as Bacillus pumilus, can solubilize phosphates, making them available to crops such as potato (Solanum tuberosum L. cv. Citlali). In this paper was studied the effect of Bacillus sp. strain Fo03, a phosphate solubilizer bacterial strain, on the growth promotion, production, and sprouting of potatoes under greenhouse conditions, compared with inorganic fertilizer. Five treatments were assayed: NPK 100% (T1), NK 100% (T2), Bacillus sp. strain Fo03, BP (T3), BP + NPK 50% (T4), and BP + NK 50% (T5). The parameters evaluated were plant height, fresh and dry weights, number of internodes, SPAD values, fresh weight of tubers, total number of tubers, number of tubers with diameters greater than or equal to 15 mm, length and width of sprouts. Treatments T1 and T4 showed a similarity in plant height (21.65 cm, 20.95 cm), number of internodes (12.42, 13.11), fresh weight (9.97 g, 5.53 g), and dry weight (1.87 g, 1.08 g), respectively. The tuber fresh weight (g), number of total tubers, tuber diameter ≥ 15 mm, and sprout width were statistically significant in T4 compared to the treatment with Bacillus sp. strain Fo03. The highlight result was the effect combined of Bacillus sp. strain Fo03 and the half dose of mineral fertilizer (NPK 50%) for having good yield of potato cultivation (growth, production, and sprouting), providing to farmers an alternative response for decreasing use of inorganic fertilizers. The use of Bacillus sp. strain Fo03 as biofertilizer may have a promising effect on the quality of potatoes, particularly in sprout length and width.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 5","pages":"228"},"PeriodicalIF":2.3,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143771705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Experimental and Computational Insights into Polyurethane Plastic Waste Conversion to Microbial Bioplastic.","authors":"Khouloud Hammami, Amal Souii, Wafa Hassen, Habib Chouchane, Ahmed Slaheddine Masmoudi, Ameur Cherif, Yasmine Souissi, Mohamed Neifar","doi":"10.1007/s00284-025-04218-w","DOIUrl":"10.1007/s00284-025-04218-w","url":null,"abstract":"<p><p>In this study, a seven-factor Hoke experimental design and the response surface methodology were used to optimize the fermentation conditions for the maximum polyhydroxyalkanoates (PHA) yield using polyurethane plastic waste (PUPW) as a source of carbon and energy for the microbial growth and biobased polyester production. The highest PHA yield (0.80 g/L ± 0.01) was obtained under a pH of 8; a temperature of 35 °C; a NaCl concentration of 5%; a PUPW concentration of 1%; an inoculum size of 15%, a monoculture of Pseudomonas rhizophila S211; and an incubation time of 6 days. The response values predicted by the Hoke design model at each combination of factor levels aligned with the experimental results, and the analysis of variance demonstrated the predictability and accuracy of the postulated model. In addition to the experimental evidences, P. rhizophila genome was explored to predict the PUPW-degrading enzymes and the associated protein secretion systems. Moreover, physicochemical properties, phylogenetic analysis, and 3D structure of S211 LipA2 polyurethanase were elucidated through an in-silico approach. Taken all together, integrated experimental tests and computational modeling suggest that P. rhizophila S211 has the necessary enzymatic machinery to effectively convert the non-biodegradable PUPW into PHA bioplastics.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 5","pages":"227"},"PeriodicalIF":2.3,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143771720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study of Stratification of Prokaryotic Microbial Communities in Sediments of Velikaya Salma Strait and Kandalaksha Bay, the White Sea.","authors":"Dorzhi V Badmadashiev, Aleksandra R Stroeva, Alexandra A Klyukina, Elena N Poludetkina, Elizaveta A Bonch-Osmolovskaya","doi":"10.1007/s00284-025-04199-w","DOIUrl":"10.1007/s00284-025-04199-w","url":null,"abstract":"<p><p>The stratification of prokaryotic microbial communities in shallow and deep-water sediments was investigated in two locations of the White Sea: Velikaya Salma Strait and the deep-water part of Kandalaksha Bay. Taxonomic diversity was analyzed using 16S rRNA gene-based profiling across horizontal sediment layers down to a depth of 50 cm. A total of 55 samples were collected from 15 stations: 43 from 11 shallow-water stations (including 4 in \"gas cap\" areas) at 2-50 cm depths and 12 from 4 deep-water stations at 2-30 cm. CH₄ concentrations were measured for all stations, while total organic carbon (TOC) content was determined for selected sites. In Velikaya Salma Strait, the upper sediment layers were dominated by microorganisms involved in labile organic matter degradation and sulfate reduction, including Woeseia, Sandaracinaceae, Actinomarinales, SEEP-SRB1, and Sva0081. Deeper layers exhibited a shift toward taxa capable of degrading complex and recalcitrant substrates, such as Desulfatiglans, Hyphomicrobiaceae, and Mycobacterium, alongside uncultured microorganisms belonging to groups like SG8-4, WCHB1-81, Aerophobales, S085, JS1, and Anaerolineaceae. Notably, JS1 made up to half of the microorganisms in deeper layers, highlighting their ecological significance. In \"gas cap\"-associated sediments no pronounced stratification was observed with more homogeneous microbial community composition across all horizons. Similarly, in the deep-water part of Kandalaksha Bay, the microbial community showed minimal vertical differentiation. The dominant taxa in deep-water sediments included SEEP-SRB1, Sva0081, Sandaracinaceae, Anaerolineaceae, Woeseia, and PHOS-HE36. This study highlights the variability in microbial community structure across sediment depths and environmental conditions in Kandalaksha Bay.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 5","pages":"225"},"PeriodicalIF":2.3,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143763113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fe₂O₃@D201 Enhanced Efficiency of Food Waste Degradation by Microbial Inoculum Under Aerobic Condition.","authors":"Ying Han, Meiqi Yin, Qingrui Zhang, Lili Tian, Hao Wu, Yu Song, Xin He","doi":"10.1007/s00284-025-04215-z","DOIUrl":"10.1007/s00284-025-04215-z","url":null,"abstract":"<p><p>The global quantity of food waste (FW) is increasing at an alarming rate, making safe disposal a pressing issue in urban management. The inappropriate disposal of FW will put risks on health and environment. Aerobic degradation equipment has emerged as a promising solution for FW disposal by adding microbial agents. However, current equipment faces challenges such as long processing duration and low efficiency. Therefore, we investigated the impact of combining microbial agents with iron oxide nano-resin (Fe₂O₃@D201) on the aerobic degradation of FW. We conducted experiments using 10% microbial agents supplemented with 6% Fe₂O₃@D201 for FW degradation. Compared to the control group containing 10% microbial agents, the Fe₂O₃@D201-treated group showed higher levels of dissolved COD in the leachate, reaching 1.59 × 10⁵ mg/L. Furthermore, the microbial hydrolytic enzyme activities in FW of this group surpassed those of the control group, with cellulase activity peaking at 0.13 U compared to the control group's peak of 0.06 U. Through 16S rRNA gene amplicon sequencing, we found that Fe₂O₃@D201 significantly enriched the abundance of Bacillus, which are commonly known for their hydrolysis functions. The results indicated that Fe₂O₃@D201 enhanced FW degradation by promoting the abundance of specialized microorganisms, and thus increased the hydrolytic enzyme activity, promoting the conversion of solid macromolecules into soluble organic matter. Consequently, Fe₂O₃@D201 shows potential for application in FW treatment equipment.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 5","pages":"224"},"PeriodicalIF":2.3,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143763407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Three Novel Bacterial Species, Roseateles flavus sp. nov., Roseateles paludis sp. nov., and Roseateles hydrophilus sp. nov., Isolated from Various Water-Related Environments.","authors":"Haejin Woo, Geeta Chhetri, Inhyup Kim, Sunho Park, Hyunji Lee, Subin Yook, Taegun Seo","doi":"10.1007/s00284-025-04209-x","DOIUrl":"10.1007/s00284-025-04209-x","url":null,"abstract":"<p><p>A polyphasic study was conducted to determine the taxonomic positions of three bacterial strains, designated as strains 2.12^T, DJS-2-20^T, and UHG3^T, isolated from the water of an agricultural water pipe, wetland, and artificial lake, respectively. These strains were Gram-stain-negative, rod-shaped, and oxidase positive. The fatty acid profiles of all three strains revealed that the major fatty acids (> 10%) were C_16:0 and summed features 3 (comprising C_16:1ω6c and/or C_16:1ω7c), with strain UHG3^T additionally featuring C_16:0 3-OH as its main fatty acid. Phosphatidylethanolamine was the major polar lipid. Ubiquinone 8 (Q-8) was the only respiratory quinone detected. The genomic G + C contents were 68.5% for strain 2.12^T, 68% for strain DJS-2-20^T, and 69% for strain UHG3^T. Strains 2.12^T, DJS-2-20^T, and UHG3^T had the closest 16S rRNA gene sequence similarity with R. subflavus APW6^T (98.5%), R. puraquae CCUG 52769^T (98.2%), and R. puraquae CCUG 52769^T (99.4%), respectively. The pairwise amino acid identity, average nucleotide identity, and digital DNA-DNA hybridization values for strains 2.12^T, DJS-2-20^T, and UHG3^T with its closely related type strain of the species were below the species delineation thresholds. The genotypic and phenotypic characteristics observed in this study indicated that strains 2.12^T (= KACC 23730^T = TBRC 19013^T), DJS-2-20^T (= KACC 23731^T = TBRC 19014^T), and UHG3^T (= KACC 22636^T = TBRC 16347^T) represent three novel Roseateles species, for which we propose the names Roseateles flavus sp. nov., Roseateles paludis sp. nov., and Roseateles hydrophilus sp. nov., respectively.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 5","pages":"226"},"PeriodicalIF":2.3,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143771722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-HBs Levels 18-26 Years After the Initial Hepatitis B Vaccination Series in a Low-to-Medium Prevalence Region: Is a Booster Dose or a Second Vaccine Series Necessary?","authors":"Halil İbrahim Erdoğdu, Çiğdem Eda Balkan Bozlak, Mevhibe Başak, Sercan Kenan Başar, İhsan Topaloğlu, Kevser Tural","doi":"10.1007/s00284-025-04211-3","DOIUrl":"10.1007/s00284-025-04211-3","url":null,"abstract":"<p><p>This cross-sectional study aimed to evaluate the persistence of immunity provided by hepatitis B (Hep B) vaccine 18-26 years after primary vaccination among 628 participants. Blood samples were collected for Hep B surface antigen (HBsAg), anti-HBs antibody (anti-HBs), and core antibody (anti-HBc) analyses. For participants with anti-HBs levels of < 10 mIU/mL, booster dose was administered and anti-HBs titers were monitored 4-6 weeks after vaccination was completed. If the response to Hep B vaccine was inadequate, two additional Hep B vaccine doses were administered. Of the 628 participants, 269 were excluded based on the exclusion criteria, and 359 were included in the final analysis. Three participants were found to be HBsAg-positive, resulting in an HBsAg carrier rate of 0.83%. Moreover, 55.4% participants had anti-HBs levels of ≥ 10 mIU/mL, with a geometric mean concentration (GMC) of 82.59 mIU/mL (95% confidence interval [CI]). Among 125 participants with anti-HBs levels of < 10 mIU/mL, 93.1% reached anti-HBs levels of ≥ 10 mIU/mL after booster dose, with a GMC of 493.47 mIU/mL. A prebooster anti-HBs cutoff level of 1.28 mIU/mL was predictive for an immune response, with a sensitivity and specificity of 71.2% and 71.4%, respectively (p = 0.001, 95% CI, likelihood ratio: 2.49). Among 14 participants unresponsive to the booster dose, 78.5% responded to the second vaccine series with a GMC of 670.82 mIU/mL. Approximately 44.6% of participants required a booster dose 18-26 years after primary vaccination. Prebooster anti-HBs titration may predict the response to booster doses. Further studies with larger groups are needed to confirm these findings.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 5","pages":"223"},"PeriodicalIF":2.3,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143763404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Weissella confusa MW051433 Mitigates Hypercholesterolemia by Biliary Cholesterol Excretion: The In Vivo Evidence.","authors":"Saman Javed, Zakia Latif, Ghulam Ayesha Javed, Aneela Munir, Maria Mushtaq, Mamoona Hameed, Muhammad Arshad, Najma Arshad","doi":"10.1007/s00284-025-04173-6","DOIUrl":"10.1007/s00284-025-04173-6","url":null,"abstract":"<p><p>The current study explored cholesterol-lowering potential and the underlying mechanism (biliary cholesterol excretion) of a prebiotic (mannitol), probiotic (Weissella confusa MW051433), and their synbiotic (mannitol + W. confusa MW051433) in high fat diet induced hypercholesterolemic murine model. The experiment employed a randomized controlled design consisting of five groups: negative control, positive control and three treatment groups, with six rats in each group. Positive control and treatment groups were maintained on high fat (18.37%) diet throughout experimental period (60 days). The high-fat diet induced significant (p < 0.05) augmentation in total serum cholesterol, triglycerides, low-density lipoprotein and decrease in high-density lipoprotein within 15 days. By 60th day of experiment, it further led to elevation in liver cholesterol, and hepatic enzymes, and depletion in hemoglobin (35.85%), erythrocytes (34.78%), and hematocrit (27.31%) in PC group. ANOVA followed by Tukey's test revealed that treatment (day 15-60 of experiment) with W. confusa MW051433 and its synbiotic, significantly improved blood lipid profile, liver functions and led to remarkable increase in fecal cholesterol (70.48%, 107.85%) and cholic acid (123.21%, 100.91%) content, respectively. Histopathological findings, including hepatic lipid droplet count, adipocyte size, and cellular integrity, further indicated that the strain facilitated cholesterol reduction through biliary cholesterol excretion mechanism. While the synbiotic showed slight superiority in one parameter, the probiotic alone yielded comparable results. To our information, this is the first report on in vivo hypocholesterolemic efficacy of W. confusa MW051433. Further research exploring its role in cholesterol homeostasis through in silico modeling, and gene/protein expression, is recommended.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 5","pages":"222"},"PeriodicalIF":2.3,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143763119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Prospective Approach for Screening of Potential Evolution of Aflatoxins in Fish and Fish Feed: Molecular and Afla Fluorometry Assays.","authors":"Nada H Eidaroos, Abdelazeem M Algammal, Mahmoud Mabrok, Mamdouh Y Elgendy, Mahmoud E Elsayed, Eman M El-Diasty, Sara M El-Khamisy, Reham M El-Tarabili","doi":"10.1007/s00284-025-04213-1","DOIUrl":"10.1007/s00284-025-04213-1","url":null,"abstract":"<p><p>The transmission of aflatoxins from fish products to consumers primarily occurs through the contamination of fish flesh and feed by aflatoxigenic fungi. This study comprehensively examined the prevalence and extent of fungal contamination in the flesh of Oreochromis niloticus and Mugil cephalus, as well as in fish feed. Furthermore, it assessed the aflatoxin-producing potential of Aspergillus flavus through fluorometric and molecular assays, providing critical insights into the risks associated with mycotoxin contamination in aquaculture products. O. niloticus and M. cephalus (n = 20 for each species) and fish feed (n = 30) were collected from fish farms and feed markets in Ismailia Governorate, Egypt. The total mold counts exceeded the permissible limit in 25% of the examined O. niloticus samples and 53.3% of the examined feed samples. A. flavus was the predominant species recovered from O. niloticus (48%), M. cephalus (75%), and feed samples (32.4%). All samples tested positive for total aflatoxins using afla fluorometry. Additionally, 60% of O. niloticus and 80% of fish feed samples exceeded global mycotoxin regulatory limits. Among the 33 Aspergillus flavus isolates, only 10 were toxigenic, testing positive for the aflatoxin regulatory (aflR) gene. Sequence analysis of the aflR gene revealed a high genetic similarity between the examined strain and A. flavus strains from Italy, Egypt, and Saudi Arabia. These findings highlight fish feed as a major source of mycotic infections and aflatoxin contamination in fish flesh. Fish feed represents a primary source of mycotic infections and aflatoxin contamination of fish flesh. This source could constitute a health hazard for consumers. Therefore, implementing strict quality control measures for feed storage and processing, along with regular monitoring of aflatoxin levels in aquaculture products, is essential to mitigate health risks and ensure food safety. The combination of morphological and molecular assays is recommended for truthfully monitoring aflatoxogenic A. flavus.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 5","pages":"221"},"PeriodicalIF":2.3,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143763401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial and Biopriming Effects of Nostocales Cyanobacteria on Tomato Plants Infected with Bacterial Spot Disease.","authors":"Sajjad Ghahari, Ghorban Ali Nematzadeh, Ali Pakdin, Mohammad Roayaei Ardakani","doi":"10.1007/s00284-025-04208-y","DOIUrl":"10.1007/s00284-025-04208-y","url":null,"abstract":"<p><p>A new generation of farming inputs is being developed, designed to be more environmentally friendly and have fewer negative impacts on consumer health. Bio-based antimicrobial compounds are one such example. These compounds are used against pathogens and stimulate plant immune systems, reducing disease severity. This study evaluated suspensions and hydro-alcoholic extracts of 55 cyanobacteria from the Nostocales order for their antimicrobial effects and growth-promoting activity on tomato plants. Suspensions and extracts of 0.5 and 1 mg mL^-1 of two cyanobacteria, Nostoc sp. G-4D and Calothrix sp. G-403 were selected for their disease control capabilities and growth enhancement effects on plants. The GC-MS technique was used to investigate the chemical compounds in the hydroalcoholic extracts of two cyanobacteria species. The analysis shows that the Nostoc sp. G-4D extract contained 28 compounds, accounting for 96.04% of the total composition, while the Calothrix sp. G-403 extract contained 27 compounds, making up 93.62% of the total composition. These findings highlight the rich chemical diversity in the extracts, which might be responsible for the observed bioactivities. The predominant components of the hydroalcoholic extracts of Nostoc sp G-4D and Calothrix sp. G-403 were Hexadecanoic acid, methyl ester (28.29%) and Octadecanoic acid, methyl ester (15.89%) for the former, and Hexadecanoic acid, methyl ester (27.95%) and phytol (10.82%) for the latter.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 5","pages":"220"},"PeriodicalIF":2.3,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143751600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a Multiplex Real-Time PCR for Diagnosis of Tuberculosis and Multidrug Resistance.","authors":"Oğuz Ari, Rıza Durmaz, Sedat Vezir, Ahmet Arslanturk","doi":"10.1007/s00284-025-04196-z","DOIUrl":"10.1007/s00284-025-04196-z","url":null,"abstract":"<p><p>The aim of this study was to develop a multiplex qPCR method for diagnosis of tuberculosis and multidrug resistance. The method was constructed with two multiplex qPCR mixes. Mycobacterium tuberculosis and rifampicin (RIF) resistance were assessed in the first PCR mix and isoniazid (INH) resistance in the second PCR mix. Firstly, PCR amplification conditions and concentration of primers and probes were optimized. Then, analytical sensitivity/limit of detection (LOD), analytical specificity, repeatability, accuracy, and effectiveness on clinical samples were analyzed. The LOD value was determined as 124 cfu/mL. In studies conducted on 273 clinical samples, RIF and INH resistance was detected with ≥ 99% sensitivity and specificity. No false positive or negative results were obtained in the accuracy studies. In intra-experiment, inter-experiment, and inter-lot repeatability studies, the coefficient of variation was calculated as between 0.36 and 5.3%. It was also determined that the primers/probes used in this assay did not cross-react with microorganisms other than M. tuberculosis complex. In conclusion, the method optimized in the current study has high performance characteristics indicating that it can be used as an alternative for currently available molecular methods for rapid and accurate diagnosis of pulmonary tuberculosis and multidrug resistance without needing any specific equipment. Further study with different clinical samples will be useful to provide more data regarding routine utility of this method.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 5","pages":"219"},"PeriodicalIF":2.3,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143735786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}