Current Microbiology最新文献

{"title":"Molecular Characterization of Enterococcus faecium Clinical Isolates Harbouring erm (T) from an Italian Hospital.","authors":"Marzia Cinthi, Sonia N Coccitto, Serena Simoni, Gloria D'Achille, Guido Zeni, Annarita Mazzariol, Antonella Pocognoli, Silvia Di Lodovico, Mara Di Giulio, Gianluca Morroni, Marina Mingoia, Carla Vignaroli, Andrea Brenciani, Eleonora Giovanetti","doi":"10.1007/s00284-024-03968-3","DOIUrl":"10.1007/s00284-024-03968-3","url":null,"abstract":"<p><p>The presence of erm(T) gene conferring resistance to macrolides, lincosamides and streptogramin B (MLS_B), was screened in 296 enterococci collected from clinical samples in a central Italy hospital and seven Enterococcus faecium isolates resulted positive to erm(T) by PCR. All isolates were resistant to erythromycin, tetracycline, ciprofloxacin and ampicillin but susceptible to vancomycin and chloramphenicol. Whole Genome Sequencing analysis revealed that in five E. faecium isolates, all belonging to the sequence type ST80 included in the clonal complex CC17 responsible of nosocomial infections, erm (T) gene was chromosome-located, in different genetic contexts. In E. faecium 735,236, erm (T) was on a 4,159-bp region flanked by two IS1216 and inserted at the 3' end of the mp gene. In E. faecium 711,448 and 739,437, erm (T) was found in a 4,463-bp region identical to that detected in E. faecium 735,236 except for 319 bp. In E. faecium 713,729 and 757,415, erm (T) was on a 7,038-bp region flanked by IS1251 and ISEfm2 transposases and encompassed between the genes encoding a recombinase and three hypothetical proteins. erm(T)-carrying minicircles were detected in all isolates by inverse PCR assays demonstrating that erm(T) was included in mobile elements. However, in conjugation assays by filter mating, the erm(T) transferability was unsuccessful. Although macrolides are not used to treat enterococcal infections, the resistance is nonetheless widespread. These antibiotics are critically important in human medicine, but only few studies focused on erm (T)-harbouring clinical enterococci. The emergence of erm (T)-mediated erythromycin resistance among enterococci, potentially transferable to other nosocomial pathogens, should be constantly monitored.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"81 12","pages":"431"},"PeriodicalIF":2.3,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142544369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering Toxic Pollutants Breakdown Potential in Microbial Community of Chumathang Hot Spring, Ladakh, India via Shotgun Metagenome Sequencing.","authors":"Neha Saini, Mohd Aamir, Zainul Abdeen Khan, Vinay Kumar Singh, Pankaj Sah, Sharma Mona","doi":"10.1007/s00284-024-03915-2","DOIUrl":"10.1007/s00284-024-03915-2","url":null,"abstract":"<p><p>Persistent Organic Pollutants (POPs) have been in focus of research due to their massive contamination of environment and bio-accumulation. Bioremediation and high-throughput research have gained momentum to curb the harmful effects of POPs. The present research has explored the microbial diversity of Chumathang Hot Spring, Ladakh, India, through Illumina metagenomic HiSeq 4000 sequencing platform and their potential to degrade persistent pollutants, especially xenobiotics. Taxonomic characterization based on raw metagenomic data illuminated the abundance of members of Pseudomonadota and Actinomyceota. The re-construction of the microbial genomes from assembled contigs and scaffolds using de novo assembler metaSPAdes and their further annotation through contig alignment with available reference genomes elucidated the landscape of the hot spring's microbes. The predominantly occupied key genera reported were Pannonibacter and Novosphingobium. Comparative genomic analysis established evolutionary relationships and functional diversities among hot spring microbial communities. The function annotation through MG-RAST has revealed their metabolic versatility of degrading a wide array of xenobiotic compounds, including caprolactam, dioxin, chlorobenzene, benzoate, and. Further, the hydroxylating dioxygenase (Saro_3901) was identified as a pivotal component in the aromatic degradation pathways, showcasing extensive metabolic interconnectivity. Interestingly, protein interaction network analysis identified hub genes like Saro_1233 (protocatechuate 4,5-dioxygenase alpha subunit), while Saro_3057 (amidase) was noted for its critical role in network communication and control. The resilience of thermal ecosystems, evidenced by robust enzymatic activity and degradation capability among organisms with < 95% genetic similarity, underscores their potential for industrial and bioremediation exploration, emphasizing the importance of preserving and studying biodiverse habitats.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"81 12","pages":"430"},"PeriodicalIF":2.3,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142521311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of Straw Degradation and Whole Genome of Acrophialophora multiforma.","authors":"Shui Zhao, Yufeng Zhao, Keyun Song, Yanling Wang, Yingxia Lu, Chunbo Dong, Yanwei Zhang, Yanfeng Han","doi":"10.1007/s00284-024-03937-w","DOIUrl":"10.1007/s00284-024-03937-w","url":null,"abstract":"<p><p>Lignin is one of the main components in plants, which can transform value-added bioenergy and chemicals. At the same time, due to the close combination of lignin and hemicellulose in the structure, it becomes a barrier for cellulose utilization. Therefore, the effective degradation of lignin is of great significance for the utilization of these resources. In this study, the lignin degrading ability of Acrophialophora multiforma strain GZUIFR 22.397 was preliminarily investigated through straw degradation experiments and enzyme activity determination. Then, the whole genome of strain A. multiforma GZUIFR 22.397 was sequentially analyzed and annotated through multiple gene function annotation databases to comprehensively evaluate its lignin degrading potential. The results showed that the weight loss of straw reached 5.98 ± 3.95%. Laccase activity was 77.49 ± 2.65 U/L, lignin peroxidase activity was 160.57 ± 29.07 U/L, and manganese peroxidase activity was 294.83 ± 3.77 U/L. The genome of A. multiforma strain GZUIFR 22.397 spans 33.81 megabases and encompasses 9,370 genes. Among these, 6,122 genes have been annotated in the Gene Ontology (GO), 2,286 in the Cluster of Orthologous Groups of proteins (KOG), 2,283 in the Kyoto Encyclopedia of Genes and Genomes (KEGG), and 603 in the Carbohydrate Active enZYmes Database (CAZy). Concurrently, the genome analysis predicted the presence of 9 genes for laccase, 2 genes for lignin peroxidase, and 2 genes for manganese peroxidase. In summary, these results indicated that A. multiforma GZUIFR 22.397 has lignin degrading ability, and laid the foundation for deciphering the molecular mechanism of A. multiforma GZUIFR 22.397 to degrade lignin.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"81 12","pages":"429"},"PeriodicalIF":2.3,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142521310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Repositioning of Antibiotics in the Treatment of Viral Infections.","authors":"Anaíd Bustos-Hamdan, Jair Isidoro Bracho-Gallardo, Aída Hamdan-Partida, Jaime Bustos-Martínez","doi":"10.1007/s00284-024-03948-7","DOIUrl":"10.1007/s00284-024-03948-7","url":null,"abstract":"<p><p>Drug repurposing, also known as drug repositioning, is a currently tested approach by which new uses are being assigned for already tested drugs. In this case there are antibiotics that are used to combat bacterial infections. However, antibiotics are among the drugs that have been studied for possible antiviral activities. Therefore, the aim of this work is to carry out a review of the studies of antibiotics that could be repositioned for the treatment of viral infections. Among the main antibiotics that have demonstrated antiviral activity are macrolides and glycopeptides. In addition, several antibiotics from the group of tetracyclines, fluoroquinolones, cephalosporins and aminoglycosides have also been studied for their antiviral activity. These antibiotics have demonstrated antiviral activity against both RNA and DNA viruses, including the recent pandemic virus SARS-CoV-2. Some of these antibiotics were selected in addition to its antiviral activity for their immunomodulatory and anti-inflammatory properties. Of the antibiotics that present antiviral activity, in many cases the mechanisms of action are not exactly known. The use of these antibiotics to combat viral infections remains controversial and is not generally accepted, since clinical trials are required to prove its effectiveness. Therefore, there is currently no antibiotic approved as antiviral therapy. Hence is necessary to present the studies carried out on antibiotics that can be repositioned in the future as antiviral drugs.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"81 12","pages":"427"},"PeriodicalIF":2.3,"publicationDate":"2024-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11512906/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142497085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent Advancements in Production and Extraction Methods of Phycobiliprotein C-phycocyanin by Arthrospira (Spirulina) platensis: A Mini Review.","authors":"Luca Usai, Serenella Torre, Nazlim Aktay, Nurhan Turgut Dunford, Valentina Citi, Lorenzo Flori, Paola Nieri, Giovanni Antonio Lutzu","doi":"10.1007/s00284-024-03964-7","DOIUrl":"10.1007/s00284-024-03964-7","url":null,"abstract":"<p><p>Arthrospira platensis has been utilized as a food source since ancient times due to its rich nutrient profile. In recent years, its popularity as a dietary supplement has soared, especially due to the presence of a water-soluble phycobiliprotein, C-phycocyanin C (C-PC), which is abundant and notable for its fluorescent properties. C-PC contains the chromophore phycocyanobilin B (PCB-B), a tetrapyrrole molecule, that is why it plays a dual role as a food colorant and as nutraceutical. However, comprehensive studies have mostly evaluated C-PC's broader health-promoting properties, particularly its antioxidative and anti-inflammatory effects, which are linked to its ability to contrast oxidative stress and related pathological conditions. That is why this review explores recent advancements in optimizing culture conditions to enhance C-PC and PCB-B production, with a particular emphasis on novel extraction and purification techniques that increase yield and bioactivity. This focus on efficient production methods is crucial for expanding the commercial and therapeutic applications of C-PC, contributing to its growing relevance in the food and pharmaceutical industries.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"81 12","pages":"428"},"PeriodicalIF":2.3,"publicationDate":"2024-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142497084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Luteibacter sahnii sp. nov., A Novel Yellow-Colored Xanthomonadin Pigment Producing Probiotic Bacterium from Healthy Rice Seed Microbiome.","authors":"Gagandeep Jaiswal, Rekha Rana, Praveen Kumar Nayak, Rekha Chouhan, Sumit G Gandhi, Hitendra K Patel, Prabhu B Patil","doi":"10.1007/s00284-024-03950-z","DOIUrl":"10.1007/s00284-024-03950-z","url":null,"abstract":"<p><p>To explore the rice seed microbiome, our objective was to isolate novel strains of Xanthomonas, a plant-associated bacterium with diverse lifestyles. Four isolates, anticipated to be Xanthomonas based on morphological features of yellow colonies, were obtained from healthy rice seeds. Phylo-taxono-genomic analysis revealed that these isolates formed monophyletic lineages belonging to a novel species within the genus Luteibacter. Pairwise ortho Average Nucleotide Identity and digital DNA-DNA hybridization confirmed their distinct species status. We propose Luteibacter sahnii sp. nov. as a novel species, with PPL193^T = MTCC 13290^T = ICMP 24807^T = CFBP 9144^T as the type strain and PPL201, PPL552, and PPL554 as other constituent members. The fatty acid profile of the type strain is dominated by branched fatty acids like Iso-C_15:0, consistent with other members of the genus. The novel species displays non-pathogenic attributes and exhibits plant probiotic properties, protecting rice plants from the leaf blight pathogen X. oryzae pv. oryzae. Production of Indole-3-Acetic Acid (IAA) and genomic regions encoding anti-microbial peptides emphasize its potential contributions to plant hosts. This study underscores the importance of employing a combination of phenotypic and genotypic methods in culturomics to enhance our understanding of rice seed microbiome diversity.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"81 12","pages":"424"},"PeriodicalIF":2.3,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142497082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial Activity of Corynebacterium amycolatum ICIS 53 and Corynebacterium amycolatum ICIS 82 Against Urogenital Isolates of Multidrug-Resistant Staphylococcus aureus.","authors":"Irina V Gladysheva, Elena A Stroganova, Konstantin L Chertkov, Sergey V Cherkasov","doi":"10.1007/s00284-024-03936-x","DOIUrl":"10.1007/s00284-024-03936-x","url":null,"abstract":"<p><p>Intermicrobial interactions play a key role in the regulation of microbial populations and the colonization of various ecological niches. In the present study, we assessed the effect of cell-free supernatants (CFSs) from the vaginal isolates Corynebacterium amycolatum ICIS 53 and Corynebacterium amycolatum ICIS 82 on urogenital test strain biofilm formation of Staphylococcus aureus. Our studies showed that the CFSs of both C. amycolatum strains significantly reduced biofilm formation and disrupted preformed S. aureus biofilms. Pretreatment with C. amycolatum ICIS 53 or C. amycolatum ICIS 82 CFSs decreased the cell surface hydrophobicity and exopolysaccharide production of all the test S. aureus isolates. The scanning electron microscopy (SEM) results showed that the CFSs of corynebacteria caused the S. aureus biofilms to be small clusters scattered across the surface, there were no fibres or adhesions between cells, and the cell membrane was not damaged. Treatment of preformed biofilms with CFSs from both C. amycolatum strains resulted in a flat, scattered, and unstructured architecture. The S. aureus cell membrane was damaged. GC‒MS analysis of the CFS of C. amycolatum ICIS 53 revealed the presence of 22 chemical compounds, including long-chain fatty alcohols, esters, fatty acids and heterocyclic pyrrolizines and pyrazoles, that, according to the literature, exhibit a wide range of biological activities. The results of the present work provide insight for the study of Corynebacterium microorganisms as a source of multifunctional bioactive compounds, which may find promising applications in the medical, biotechnological and pharmaceutical industries.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"81 12","pages":"426"},"PeriodicalIF":2.3,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142497080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and Characterization of Arsenic-Tolerable Bacteria from Groundwater and Their Implementation on Rice Seedling's Shoot and Root Enhancement.","authors":"Ashutosh Kabiraj, Urmi Halder, Rajib Bandopadhyay","doi":"10.1007/s00284-024-03951-y","DOIUrl":"10.1007/s00284-024-03951-y","url":null,"abstract":"<p><p>Arsenic exerts detrimental impacts on primary metabolism in plants, leading to reduced crop yield. Some arsenic-resistant plant growth-promoting bacteria (PGPB) help plants by providing some plant hormones to sustain their growth and development under arsenic stress. Here, seven different species of Bacillus were isolated from arsenic-contaminated groundwater of West Bengal, India. Those species were capable of growing in the presence of > 3.12 g/L arsenate (AsV) and > 0.65 g/L arsenite (AsIII) salts and also resist different heavy metals like Cu²⁺, Fe²⁺, Co²⁺, Zn²⁺, Pb²⁺, etc. They were susceptible to multiple groups of antibiotics like beta-lactam, aminoglycosides, etc. All species were capable of detoxifying arsenite and influenced rice seedlings' growth in the presence of arsenic salts by their capabilities like nitrogen-fixing ability, phosphate solubilization, indole 3-acetic acid (IAA), gibberellic acid (GA), proline production, etc. Most species helped enhance root and shoot lengths under arsenic stress. These primary findings suggest that those Bacillus spp. could be used as potential bio-fertilizers in arsenic-contaminated agricultural fields.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"81 12","pages":"425"},"PeriodicalIF":2.3,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142497081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photorhabdus viridis sp. nov. Isolated from Heterorhabditis zealandica Entomopathogenic Nematodes.","authors":"Ricardo A R Machado, Antoinette P Malan, Joaquín Abolafia, Jaspher Ewany, Aashaq Hussain Bhat, S Patricia Stock","doi":"10.1007/s00284-024-03935-y","DOIUrl":"10.1007/s00284-024-03935-y","url":null,"abstract":"<p><p>A novel bacterial species, Photorhabdus viridis sp. nov., represented by strain Green^T, isolated from Heterorhabditis zealandica MJ2C entomopathogenic nematodes, is described. Phylogenetic reconstructions using 16S rRNA gene sequences show that strain Green^T is closely related to P. thracensis DSM 15199^T. The 16rRNA gene sequences of these two strains are 98.8% identical. Phylogenetic reconstructions using whole-genome sequences show that strain Green^T is closely related to P. tasmaniensis DSM 22387^T, P. thracensis DSM 15199^T, and P. temperata DSM 14550^T. Digital DNA-DNA hybridization (dDDH) values between strain Green^T and its three more close relative species, P. tasmaniensis DSM 22387^T, P. thracensis DSM 15199^T, and P. temperata DSM 14550^T, are 49%, 59%, and 59%, respectively. In addition, average nucleotide identity (ANI) values between Green^T and P. tasmaniensis DSM 22387^T, P. thracensis DSM 15199^T, and P. temperata DSM 14550^T are 92.4%, 94.4%, and 94.6%, respectively. The novel species also differs in their biochemical capacities from the biochemical capacities of their more closely related taxa. The following biochemical tests may be particularly useful in this context: Arginine dihydrolase, gelatinase, and glucose and mannitol oxidation. Given the clear phylogenetic separation, the sequence divergence values, and the phenotypic differences, we conclude that strain Green^T represents a novel bacterial species, for which we propose the name Photorhabdus viridis sp. nov. with Green^T (= CCM 9407^T = CCOS 2117^T = MJ2C^T) as the type strain. Our study contributes to a better understanding of the taxonomy and biodiversity of an important bacterial group with great biotechnological and agricultural potential.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"81 12","pages":"423"},"PeriodicalIF":2.3,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11499390/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142497083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Reverse Ecology-Based Approach to Design a Bacterial Consortium as Soybean Bioinoculant.","authors":"Osiel S Gonçalves, Alexia S Fernandes, Mateus F Santana","doi":"10.1007/s00284-024-03926-z","DOIUrl":"10.1007/s00284-024-03926-z","url":null,"abstract":"<p><p>Bioinoculants traditionally rely on selecting efficient microbes from the soil with potential growth-enhancing traits for plants. However, such approaches often neglect microbe-microbe and microbe-plant interactions. In this study, we applied a reverse ecology framework to design and assess a bacterial consortium tailored for soybeans. Our analysis identified Paenibacillus polymyxa, Methylobacterium brachiatum, and Enterobacter sp. as key strains for their synergistic potential in promoting soybean growth. Computational analyses revealed that these selected strains exhibited low competitiveness and metabolic compatibility. Specifically, their complementary metabolic profiles suggested minimal competition for resources and potential for mutualistic interactions. In vitro experiments further supported these findings, demonstrating that the consortium maintained stable growth without inhibitory effects among strains. In addition, greenhouse validation experiments confirmed the efficacy of the microbial consortium in enhancing soybean growth such as root and shoot development and biomass production. Overall, this study underscores the potential of reverse ecology in optimizing microbial consortia design for bioinoculant applications.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"81 12","pages":"421"},"PeriodicalIF":2.3,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142497086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}