Whole Genome Sequencing-Based Taxonomic Identification of Dulcitol-Positive Cronobacter dublinensis subsp. beijingensis subsp. nov. and Cronobacter dublinensis subsp. infanticibi subsp. nov. from Infant Cereals in China.

IF 2.6 3区生物学 Q3 MICROBIOLOGY

Current Microbiology Pub Date : 2025-08-26 DOI:10.1007/s00284-025-04447-z

Xin Gan, Menghan Li, Fengqin Li, Jianyun Zhao, Hui Li, Séamus Fanning, Yinping Dong, Li Bai

{"title":"Whole Genome Sequencing-Based Taxonomic Identification of Dulcitol-Positive Cronobacter dublinensis subsp. beijingensis subsp. nov. and Cronobacter dublinensis subsp. infanticibi subsp. nov. from Infant Cereals in China.","authors":"Xin Gan, Menghan Li, Fengqin Li, Jianyun Zhao, Hui Li, Séamus Fanning, Yinping Dong, Li Bai","doi":"10.1007/s00284-025-04447-z","DOIUrl":null,"url":null,"abstract":"Cronobacter, a Gram-negative opportunistic pathogen, poses a significant risk to neonates. The taxonomic position of strains CFSA2021A14T and CFSA2021A84T, isolated from cereal-based infant noodles in China, was investigated. Phenotypic and genotypic analyses identified both strains as novel subspecies of Cronobacter dublinensis, with dulcitol utilization attributed to the presence of the gatYZABCD gene cluster. Based on ANI analysis, the strains are proposed as Cronobacter dublinensis subsp. beijingensis subsp. nov. and Cronobacter dublinensis subsp. infanticibi subsp. nov. The type strain C. dublinensis subsp. beijingensis CFSA2021A14T (= CGMCC 1.61450T, = JCM 35886T) grew aerobically at 25-41 °C, with negative myo-inositol and amygdalin utilization. Strain CFSA2021A14T genome size was 4.71 Mbp with a G + C content of 57.2%. The type strain C. dublinensis subsp. infanticibi CFSA2021A84T (= CGMCC 1.61451T, = JCM 35887T) exhibited similar growth conditions to C. dublinensis subsp. beijingensis CFSA2021A14T, but showed positive myo-inositol and amygdalin utilization. Strain CFSA2021A84T genome size was 4.73 Mbp with a G + C content of 57.4%.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 10","pages":"477"},"PeriodicalIF":2.6000,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12380951/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s00284-025-04447-z","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Cronobacter, a Gram-negative opportunistic pathogen, poses a significant risk to neonates. The taxonomic position of strains CFSA2021A14^T and CFSA2021A84^T, isolated from cereal-based infant noodles in China, was investigated. Phenotypic and genotypic analyses identified both strains as novel subspecies of Cronobacter dublinensis, with dulcitol utilization attributed to the presence of the gatYZABCD gene cluster. Based on ANI analysis, the strains are proposed as Cronobacter dublinensis subsp. beijingensis subsp. nov. and Cronobacter dublinensis subsp. infanticibi subsp. nov. The type strain C. dublinensis subsp. beijingensis CFSA2021A14^T (= CGMCC 1.61450^T, = JCM 35886^T) grew aerobically at 25-41 °C, with negative myo-inositol and amygdalin utilization. Strain CFSA2021A14^T genome size was 4.71 Mbp with a G + C content of 57.2%. The type strain C. dublinensis subsp. infanticibi CFSA2021A84^T (= CGMCC 1.61451^T, = JCM 35887^T) exhibited similar growth conditions to C. dublinensis subsp. beijingensis CFSA2021A14^T, but showed positive myo-inositol and amygdalin utilization. Strain CFSA2021A84^T genome size was 4.73 Mbp with a G + C content of 57.4%.

Abstract Image

查看原文本刊更多论文

dulcitol阳性都柏林克罗诺杆菌亚种的全基因组分类鉴定。beijingensis无性系种群。11 .和都柏林克罗诺杆菌亚种。infanticibi无性系种群。11月来自中国的婴儿谷物。

克罗诺杆菌是一种革兰氏阴性机会致病菌，对新生儿有重大危险。研究了从中国谷物婴儿面中分离得到的CFSA2021A14T和CFSA2021A84T菌株的分类位置。表型和基因型分析表明，这两株菌株都是都柏林克罗诺杆菌的新亚种，它们对dulcitol的利用归因于gatYZABCD基因簇的存在。根据ANI分析，该菌株为都柏林克罗诺杆菌亚种。beijingensis无性系种群。11 .和都柏林克罗诺杆菌亚种。infanticibi无性系种群。11 .类型菌株C. dublinensis亚种。beijingensis CFSA2021A14T （= CGMCC 1.61450T, = JCM 35886T）在25-41℃条件下有氧生长，肌醇和苦杏仁苷利用率为负。菌株CFSA2021A14T基因组大小为4.71 Mbp， G + C含量为57.2%。类型菌株C. dublinensis亚种。infanticibi CFSA2021A84T （= CGMCC 1.61451T, = JCM 35887T）的生长条件与C. dublinensis亚种相似。北京ensis CFSA2021A14T对肌醇和苦杏仁苷的利用呈阳性。菌株CFSA2021A84T基因组大小为4.73 Mbp， G + C含量为57.4%。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Current Microbiology 生物-微生物学

CiteScore

4.80

自引率

3.80%

发文量

380

审稿时长

2.5 months

期刊介绍： Current Microbiology is a well-established journal that publishes articles in all aspects of microbial cells and the interactions between the microorganisms, their hosts and the environment. Current Microbiology publishes original research articles, short communications, reviews and letters to the editor, spanning the following areas: physiology, biochemistry, genetics, genomics, biotechnology, ecology, evolution, morphology, taxonomy, diagnostic methods, medical and clinical microbiology and immunology as applied to microorganisms.