Current Microbiology最新文献

{"title":"Antimicrobial Efficacy of Cloxacillin-Loaded Chitosan Nanoparticles Against Staphylococcus aureus Biofilms in Subclinical Mastitis.","authors":"Mohammadsaeed Eskandari, Zohreh Abdolmaleki, Farhad Moosakhani, Mohammad Amin Eslampour","doi":"10.1007/s00284-024-04030-y","DOIUrl":"10.1007/s00284-024-04030-y","url":null,"abstract":"<p><p>Bovine mastitis is the most widespread disease that causes financial loss in the dairy industry. Staphylococcus aureus is a well-researched multidrug-resistant opportunistic bacterium that is frequently linked to subclinical mastitis and causes significant economic losses. A further problem in the management of S. aureus infections is its capacity to form biofilms; bacteria inside biofilms exhibit greater resistance to antimicrobials than planktonic cells. The most effective method for controlling mastitis is antibiotic therapy. Cloxacillin (CLX), ampicillin, and ceftiofur are currently the most often utilized drying treatments for dairy cattle. We have evaluated the therapy efficiency of cloxacillin-loaded chitosan nanoparticles (CLX-CS NPs) as well as the relationship between biofilm production, gene profile and the type of trial group (CLX and CLX-CS NPs) against S.aureus isolated from milk samples of cows diagnosed with subclinical mastitis. Investigation of phenotypic biofilm production showed that majority of the S. aureus isolates extracted from milk were producers of biofilm. Cloxacillin-loaded chitosan nanoparticles were able to significantly decrease the MIC (p < 0.05) and MBC values compared to cloxacillin. The relationship between the gene profile and the types of trial groups shows that coating cloxacillin with chitosan nanoparticles (CS-NPs) was able to reduce the MIC and MBC value in all nine gene profile groups. These findings indicate that administration of cloxacillin-loaded CS-NPs in the treatment of mastitis may improve cloxacillin therapeutic properties and could act as a potential alternative to the cloxacillin antibiotic for the treatment of bovine mastitis.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 2","pages":"65"},"PeriodicalIF":2.3,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142926766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physicochemical Characterization of Gallstone Surfaces to Predict Their Interaction with Salmonella Typhi.","authors":"Fadoua Louglali, Abdeslam Jaafari, Souad Lekchiri, Hakim Taoufik, Chorouk Zanane, Iman Meftah, Ahmed Liba, Mostafa El Louali, Hafida Zahir, Hassan Latrache","doi":"10.1007/s00284-024-04052-6","DOIUrl":"10.1007/s00284-024-04052-6","url":null,"abstract":"<p><p>Salmonella Typhi can adhere to and build biofilms on the surface of gallstones causing abnormal gallbladder mucosa, which could lead to carcinogenesis. The surface physicochemical properties of microbial cells and materials have been shown to play a crucial role in adhesion. Therefore, the purpose of this study was to investigate, for the first time, the surface properties of nine gallstones and to evaluate the influence of these parameters on the theoretical adhesion of S. Typhi to gallstone surfaces. The physicochemical properties were determined by SEM-EDX and contact angle measurements (CAM) while the predictive adhesion of S. Typhi on gallstones was estimated using the XDLVO approach. SEM-EDX analysis revealed that cholesterol is the principal component on the surface of all gallstones, with carbon and oxygen as the main elements. Aluminum was detected as a trace element in only three gallstones: GS2, GS4, and GS5. S. Typhi CIP5535 has a hydrophilic character (ΔG_iwi = 33.54 mJ m^-2), as well as strong electron donor (γ^- = 55,80 mJ m^-2) and weak electron acceptor properties (γ⁺  = 1,95 mJ m^-2). Regarding gallstones, it was found that they have a hydrophobic character (ΔG_iwi between -29,9 mJ m^-2 and -75,2 mJ m^-2), while their electron donor/acceptor characters change according to each gallstone. Predictive adhesion showed that all gallstones could be colonized by S. Typhi <math> <mfenced><mrow><mi>Δ</mi> <msubsup><mi>G</mi> <mrow><mtext>XDLVO</mtext></mrow> <mtext>Total</mtext></msubsup> <mo><</mo> <mn>0</mn></mrow> </mfenced> </math> except GS1, GS5, and GS6 <math> <mfenced><mrow><mi>Δ</mi> <msubsup><mi>G</mi> <mrow><mtext>XDLVO</mtext></mrow> <mtext>Total</mtext></msubsup> <mo>></mo> <mn>0</mn></mrow> </mfenced> </math> . Understanding the interfacial phenomena implicated in the process of bacterial adhesion makes it possible to limit or even inhibit the adhesion of S. Typhi on gallstone surfaces.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 2","pages":"67"},"PeriodicalIF":2.3,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142926792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Response Surface D-Optimal Design for Optimizing Fortimicins Production by Micromonospora olivasterospora and New Synergistic Fortimicin-A-Antibiotic Combinations.","authors":"Heba Mohammed Refat M Selim, Fatma Alzahraa M Gomaa, Mohammad Y Alshahrani, Khaled M Aboshanab","doi":"10.1007/s00284-024-04049-1","DOIUrl":"10.1007/s00284-024-04049-1","url":null,"abstract":"<p><p>Fortimicins (FTMs) are fortamine-containing aminoglycoside antibiotics (AGAs) produced by M. olivasterospora DSM 43868 with excellent bactericidal activities against a wide range of Enterobacteriaceae and synergistic activity against multidrug-resistant (MDR) pathogens. Fortimicin-A (FTM-A), the most active member of FTMs, has the lowest susceptibility to inactivation by the aminoglycoside modifying enzymes (AMEs). Therefore, this study aimed to evaluate the antibacterial activity of FTM-A alone or in combination with other antibiotics against 18 non-clonal clinically relevant MDR Gram-positive and Gram-negative pathogens. This study also aimed to statistically optimize various environmental factors affecting its production using the response surface D-optimal design. Results showed that FTM-A/meropenem combination showed the highest synergistic bactericidal activity (61.1%) followed by its combination with cefotaxime and cefepime (38.8% each). However, FTM-A/gentamicin and FTM-A/doxycycline combinations showed mostly additive effects in 66.6% and 50% of the tested isolates, respectively. For FTM-A production optimization, maximum specific activity (µg/mg) to cell growth was achieved using aminoglycoside production medium followed by yeast extract-malt extract and M65 production medium. A D-optimal quadratic model consisting of 27 different media composition variations was used to predict an optimal composition for FTM-A production and verified experimentally. Lab verification of the model was carried out using HPLC analysis, resulting in a 10.5-fold increase in their production compared to the un-optimized conditions. The model revealed that the initial pH, incubation temperature, and incubation time significantly affected FTMs production (P-value < 0.05), however, the tested range of calcium carbonate 2-7 gL-1 and agitation rate (100-300 rpm) showed no significant effect (P-value > 0.05). In conclusion, the D-optimal design resulted in an effective model and optimized FTMs production on the shake flask level. FTM-A combinations with meropenem, cefotaxime, cefepime, and gentamicin showed mostly synergistic/additive effects and are advised for clinical evaluation.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 2","pages":"68"},"PeriodicalIF":2.3,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142926794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and Characterization of a Promising Lignocellulolytic Enzyme Producer Pseudolagarobasidium acaciicola SL3-03 from Mangrove Soil in Thailand.","authors":"Chanaphon Jadtanim, Thi Thu Huong Luong, Supattra Poeaim","doi":"10.1007/s00284-024-04029-5","DOIUrl":"10.1007/s00284-024-04029-5","url":null,"abstract":"<p><p>Lignocellulolytic enzymes isolation from mangrove-derived organisms has many industrial advantages due to their efficiency in dealing with extreme and challenging conditions, such as high temperatures and salt concentrations. This study aimed to isolate fungal enzyme producers from mangrove soil in Thailand to produce lignocellulolytic enzymes (carboxymethyl cellulase: CMCase, xylanase, and laccase) and to characterize these enzymes to support industrial applications. Forty-eight fungi were isolated from the mangrove samples, and their enzyme-producing capabilities were assessed using primary and secondary screening methods. The findings revealed that Pseudolagarobasidium acaciicola SL3-03 emerged as a promising producer of lignocellulolytic enzymes. It exhibited the ability to produce 1.345 U/mL of CMCase, 1.293 U/mL of xylanase, and 43.126 U/mL of laccase. Furthermore, the enzymatic characteristics of P. acaciicola SL3-03 were analyzed. The CMCase exhibited optimal activity at 50 °C and pH 5.5, the xylanase at 50 °C and pH 4.8, and the laccase at 55 °C and pH 5. Besides, the CMCase and xylanase from P. acaciicola SL3-03 expressed high halotolerance abilities that could maintain activity and stability under high salt concentrations (149% activity at 5 M NaCl). Future studies may focus on structural analysis of the enzymes to further characterize and identify their specific types. The results suggest that mangrove soil harbors significant potential for discovering proficient lignocellulolytic enzyme producers with desirable characteristics, which can be advantageous for industrial applications.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 1","pages":"62"},"PeriodicalIF":2.3,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142909310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Leifsonia stereocauli sp. nov., Isolated from Stereocaulon tomentosum Fr. Lichen.","authors":"Shu-Ya Zuo, Lian-Shuang Xiong, Qing Yuan, Guo-Hua Chen, Rui Yang, Li-Song Wang, Cheng-Lin Jiang, Xin-Yu Wang, Yi Jiang","doi":"10.1007/s00284-024-04040-w","DOIUrl":"10.1007/s00284-024-04040-w","url":null,"abstract":"<p><p>A Gram-stain-positive, aerobic, yellow-pigmented, catalase-positive, oxidase-positive, non-motile with no flagella and irregularly rod-shaped, denominated strain YIM 134122^T, was isolated from a Stereocaulon tomentosum Fr. lichen gathered on Baima Snow Mountain in Diqing Tibetan Autonomous Prefecture, Yunnan Province, China. The novel strain grew at pH 6.0-8.0 (optimum, pH 7.0-7.2), 4-35 ℃ (optimum,25-35 ℃), and with the existence of 0-10% (w/v) NaCl (optimum, 0-2%). Phylogenetic analysis on the basis of 16S rRNA gene sequences indicated that strain YIM 134122^T belonged to the genus Leifsonia and presented high levels of 16S rRNA gene sequence similarity with Leifsonia kafniensis JCM 17021^T (97.92%) and Leifsonia psychrotolerans CGMCC 1.12166^T (97.79%). Based on the draft genome sequence, the G + C content of strain YIM 134122^T was found to be 68.41%, and the average nucleotide identity and digital DNA-DNA hybridizations values between strain YIM 134122^T and Leifsonia kafniensis JCM 17021^T and Leifsonia psychrotolerans CGMCC 1.12166^T were 73.78% and 73.75%, and 19.6% and 19.9%, respectively, which were extremely lower than the generally proposed threshold value for species delineation. The predominant polar lipids in strain YIM 134122^T consisted of phosphatidylglycerol, diphosphatidylglycerol, three unknown glycolipids, two unknown phospholipids, and one unknown lipid, whereas MK-10, MK-11 and MK-12 were the main menaquinones. The major fatty acids (> 4%) of the strain contained iso-C_15:0, anteiso-C_15:0, iso-C_16:0 and anteiso-C_17:0. On the basis of phylogenetic, chemotaxonomic, phenotypic and genotypic evidences, it is concluded that strain YIM 134122^T represents a novel species of the genus Leifsonia, for with the name Leifsonia stereocauli sp. nov. is proposed. The type strain is YIM 134122^T (= CGMCC 1.62033^T = MCCC 1K08590^T = KCTC 59219^T).</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 1","pages":"60"},"PeriodicalIF":2.3,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142892988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Arvimicrobium flavum gen. nov., sp. nov., A Novel Genus in the Family Phyllobacteriaceae Isolated From Forest Soil.","authors":"Hyosun Lee, Dhiraj Kumar Chaudhary, Ki-Eun Lee, In-Tae Cha, Won-Jae Chi, Sunho Park, Taegun Seo, Dong-Uk Kim","doi":"10.1007/s00284-024-04043-7","DOIUrl":"10.1007/s00284-024-04043-7","url":null,"abstract":"<p><p>During the study of microbial diversity of forest soil in the Republic of Korea, a yellow pigment-producing, Gram-stain-negative, rod-shaped, motile bacterium was isolated and designated as strain 1W2^T. This strain grew at temperature of 10-37 °C, at pH of 5.0-9.0, and at NaCl concentration of 0-3.0% (w/v). The 16S rRNA gene sequencing and genome sequencing revealed that strain 1W2^T is a member of the family Phyllobacteriaceae but exhibits low similarity with known genera, suggesting that this strain is a new genus within the family. This strain showed the closest similarity to the genera Mesorhizobium (96.6-96.9%), Aminobacter (96.4 -96.6%), Aquamicrobium (96.5-96.7%), and Pseudaminobacter (96.6-96.7%). The nearest relative of 1W2^T was Mesorhizobium shangrilense CCBAU 65327^T with the 16S rRNA gene sequence similarity of 96.9%. The genome size was 5,545,526 bp with DNA G + C content of 64.7%. The values of overall genomic relatedness indices between strain 1W2^T and the reference members were 20.4-21.3% for digital DNA-DNA hybridization, 74.0-76.6% for average nucleotide identity, and 68.1-61.2% for amino acids identity. Chemotaxonomic profiling revealed that Q-10 was the sole ubiquinone; summed feature 8 (C_18:1ω7c and/or C_18:1ω6c), iso-C_13:0, and C_19:0 cyclo ω8c were the predominant fatty acids; and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylcholine, and phosphatidylethanolamine were the major polar lipids. Based on these data, strain 1W2^T represents a novel species of a new genus in the family Phyllobacteriaceae. Accordingly, we proposed the name Arvimicrobium flavum gen. nov., sp. nov., with the type strain 1W2^T (= KCTC 92441^T = NBRC 116019^T).</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 1","pages":"61"},"PeriodicalIF":2.3,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142892985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial Dynamics in COVID-19: Unraveling the Impact of Human Microbiome on Disease Susceptibility and Therapeutic Strategies.","authors":"Soumya Kanti Guha, Sougata Niyogi","doi":"10.1007/s00284-024-04041-9","DOIUrl":"10.1007/s00284-024-04041-9","url":null,"abstract":"<p><p>This review explores the bidirectional relationship between the human microbiome and SARS-CoV-2 infection, elucidating its implications for COVID-19 susceptibility, severity, and therapeutic strategies. Metagenomic analyses reveal notable alterations in microbiome composition associated with SARS-CoV-2 infection, impacting disease severity and clinical outcomes. Dysbiosis within the respiratory, gastrointestinal, oral, and skin microbiomes exacerbates COVID-19 pathology through immune dysregulation and inflammatory pathways. Understanding these microbial shifts is pivotal for devising targeted therapeutic interventions. Notably, co-infection of oral pathogens with SARS-CoV-2 worsens lung pathology, while gut microbiome dysbiosis influences viral susceptibility and severity. Potential therapeutic approaches targeting the microbiome include probiotics, antimicrobial agents, and immunomodulatory strategies. This review underscores the importance of elucidating host-microbiota interactions to advance precision medicine and public health initiatives in combating COVID-19 and other infectious diseases.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 1","pages":"59"},"PeriodicalIF":2.3,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142885272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diversity and Distribution of Methane Functional Microorganisms in Sedimentary Columns of Hongfeng Reservoir in Different Seasons.","authors":"Chai Luo, Shuang Bai, Jing Sun, Fushun Wang, Shuwen Ma, Tian Xu, Jing Ma, Xue-Ping Chen","doi":"10.1007/s00284-024-04038-4","DOIUrl":"10.1007/s00284-024-04038-4","url":null,"abstract":"<p><p>Freshwater ecosystem is a significant natural source of CH₄ emission in the atmosphere. To fully understand the dynamics of methane emissions in reservoirs, it is essential to grasp the temporal and vertical distribution patterns, as well as the factors that influence the methanogenic bacterial communities within the sediments. This study investigates the methane dynamics, carbon isotope fractionation (δ¹³CH₄), and abundance of functional microorganisms along the geochemical gradient in the in situ sedimentary column of Hongfeng Reservoir (China). Notably, the methane concentration in sediment in summer ranged in 15.39-127.22 µmol/L, which is twice as high as wintertime concentrations in the surface layer near the sediment-water interface (0-10 cm depth). Illumina sequencing of the sediments identified 11 genera affiliated with methanogenic archaea, with dominant genus Methanosaeta reaching a relative abundance of 34.95% in summer. The total carbon (TOC) content in sedimentary columns in different seasons is positively correlated with Methanosarcina (P < 0.05). In addition, seasonal discrepancies are observed in the sediment profiles for total nitrogen (TN), sulfate (SO₄^2-), and ferrous iron (Fe²⁺) concentrations. The concentration of total nitrogen (TN) is higher in summer than in winter. In summer, sulfate accumulates in the middle layer of the sedimentary column, while in winter, the maximum concentration of sulfate in the surface layer reaches 0.65 mmol/L. These geochemical gradients drive the biological transformation of nitrogen, sulfur, and iron, may also be linked to the consumption of methane. Thus, it is established that the temporal and spatial dynamics of methanogenic communities in sediments significantly influence the fluctuations in methane release fluxes within reservoirs, highlighting the necessity to account for seasonal biological variations when assessing greenhouse gas emissions from reservoirs.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 1","pages":"55"},"PeriodicalIF":2.3,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142881656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Soluble Membrane Attack Complex (sMAC) as a Potential Diagnostic Biomarker Differentiating Acute Viral Encephalitis from Guillain-Barré Syndrome, a Post-infectious Autoimmune State.","authors":"Akila Swaminathan, Prasad Varamballi, Srilatha Marate, Rohan V Paul, Chiranjay Mukhopadhyay, Amrita Pattanaik","doi":"10.1007/s00284-024-04039-3","DOIUrl":"10.1007/s00284-024-04039-3","url":null,"abstract":"<p><p>Acute encephalitis syndrome (AES) presents with the onset of fever, altered sensorium and/or seizures, known to be caused by various infectious and non-infectious aetiological agents, among which viruses are the commonest. The severity of AES prompts rapid diagnosis, which is not met by time-consuming conventional diagnostic techniques. In this study, archived cerebrospinal fluid samples of laboratory-confirmed viral AES, an acute infectious condition and Guillain-Barré Syndrome (GBS), a post-infectious, autoimmune condition was assessed for soluble membrane attack complex (sMAC) using ELISA. Statistical analysis was performed to understand the diagnostic potential of sMAC in AES versus GBS patients. sMAC levels were significantly increased in viral encephalitis compared with GBS samples (43.69 ng/mL vs. 29.33 ng/mL, P < 0.05). The diagnostic potential of sMAC was assessed using the receiver operating characteristic (ROC) curve, which demonstrated excellent diagnostic discrimination between viral AES and GBS (area under curve = 0.8125, 95% CI, P < 0.0001). Using Youden's index, the optimal sMAC cut-off was calculated as 33.6 ng/mL for distinguishing AES from GBS. The findings of our study revealed significant increase in sMAC levels in AES patients in comparison to those with GBS. This underscores the utility of sMAC as a valuable tool in distinguishing between AES and GBS, thereby facilitating more tailored patient management strategies, which varies for acute infectious and post-infectious conditions especially those mediated by autoimmunity.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 1","pages":"58"},"PeriodicalIF":2.3,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142880972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitigating Health Risks Through Environmental Tracking of Pseudomonas aeruginosa.","authors":"Tushar Koujalagi, Rohit Ruhal","doi":"10.1007/s00284-024-04036-6","DOIUrl":"10.1007/s00284-024-04036-6","url":null,"abstract":"<p><p>Pseudomonas aeruginosa is a prevalent nosocomial pathogen and a significant reservoir of antimicrobial resistance genes in residential and built environments. It is also widespread in various indoor and outdoor settings, including sewage, surface waters, soil, recreational waters (both treated and untreated), and industrial effluents. Surveillance efforts for P. aeruginosa are primarily focused on hospitals rather than built environments. However, evidence links multidrug-resistant P. aeruginosa of human origin with activity in built environments and hospital settings. Consequently, tracking this pathogen across all environments is crucial for understanding the mechanisms of reverse transmission from built environments to humans. This review explores public health hygiene by examining the prevalence of P. aeruginosa in various environments, its sequence types, the factors contributing to multidrug resistance, and the identification methods through global surveillance. Whole-genome sequencing with sequence typing and real-time quantitative PCR are widely used to identify and study antimicrobial-resistant strains worldwide. Additionally, advanced techniques such as functional metagenomics, next-generation sequencing, MALDI-TOF, and biosensors are being extensively employed to detect antimicrobial-resistant strains and mitigate the ongoing evolution of bacterial resistance to antibiotics. Our review strongly underscores the importance of environmental monitoring of P. aeruginosa in preventing human infections. Furthermore, strategic planning in built environments is essential for effective epidemiological surveillance of P. aeruginosa and the development of comprehensive risk assessment models.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 1","pages":"57"},"PeriodicalIF":2.3,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142880652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}