Current Microbiology最新文献_第8页

Lactiplantibacillus from Peruvian Criollo Blanco Cocoa and Cadmium Removal via a Bacterial Consortium During Cocoa Fermentation. 秘鲁Criollo Blanco可可中的乳酸菌与可可发酵过程中细菌联合体对镉的去除。

IF 2.6 3区生物学

Current Microbiology Pub Date : 2025-09-05 DOI: 10.1007/s00284-025-04471-z

Yoisy Fabiola Agurto-Hidalgo, Karen Eliana Valdiviezo-Rumiche, Rosita Tanyelisbeth Castillo-Rogel, Miriam Marleni Rosales-Cuentas, Heber Peleg Cornelio-Santiago, Jube Ciro Portalatino-Zevallos, Edwin Jorge Vega-Portalatino

{"title":"Lactiplantibacillus from Peruvian Criollo Blanco Cocoa and Cadmium Removal via a Bacterial Consortium During Cocoa Fermentation.","authors":"Yoisy Fabiola Agurto-Hidalgo, Karen Eliana Valdiviezo-Rumiche, Rosita Tanyelisbeth Castillo-Rogel, Miriam Marleni Rosales-Cuentas, Heber Peleg Cornelio-Santiago, Jube Ciro Portalatino-Zevallos, Edwin Jorge Vega-Portalatino","doi":"10.1007/s00284-025-04471-z","DOIUrl":"10.1007/s00284-025-04471-z","url":null,"abstract":"Peru is the eighth largest producer of cocoa beans worldwide; however, the high cadmium content (Cd) presented in the white Criollo cocoa beans from the Piura region, has limited their commercialization. A potential strategy to mitigate this problem is the application of native lactic acid bacteria (LAB), capable of reducing Cd during the fermentation stage of the grain. Three Theobroma cacao L. fruits of the Creole variety were purchased from six farmers located in Malingas, district of Tambogrande, Piura, Perú. LAB were isolated, and their biosecurity and technological properties were assessed for potential use in the fermentation of cocoa beans derived from two phylogenetically characterized consortia 16S rDNA M1 (Lactiplantibacillus sp. COE1, L. plantarum COE4 and Lactiplantibacillus sp. COB2) and M2 (Lactiplantibacillus sp. COE1, L. plantarum L. plantarum COE4 and Lactiplantibacillus sp. CCC5). The concentration of cadmium was subsequently assessed. Twenty-four strains were isolated; however, seven were discarded because they were Gram-negative and presented positive hemolysis. Of the 17 LAB, 41% of the bacteria presented strong inhibition on Listeria monocytogenes, Salmonella enterica sv typhimurium, Escherichia coli ATCC10536, E. coli O157:H7 and Candida albicans. COE1 and COE2 presented high acidifying activity, COE3 high proteolytic activity, CCC4 high lipolytic activity, COE3 and COE5 high diacetyl synthesis, COB2, CCC5, COE1 and COE4 presented high resistance to cadmium (50 mg/L). Four strains were identified, two of which belonged to the genus Lactiplantibacillus (CCC5 and COE1) and two to the species L. plantarum (COE4 and COB2). M2 reduced the Cd content in dry cocoa beans with 8.5% moisture content by 3.89%.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 10","pages":"491"},"PeriodicalIF":2.6,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144999890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptomic Analysis Reveals Key Regulatory Genes and Pathways Involved in Macrophage Inflammation Induced by Hypervirulent Klebsiella pneumoniae Infection. 转录组学分析揭示了高毒力肺炎克雷伯菌感染诱导巨噬细胞炎症的关键调控基因和途径。

IF 2.6 3区生物学

Current Microbiology Pub Date : 2025-09-05 DOI: 10.1007/s00284-025-04481-x

Jinli Bi, Xin Huang, Liqin Meng, Jinghan Li, Yu He, Jing Jin, Taijie Li

{"title":"Transcriptomic Analysis Reveals Key Regulatory Genes and Pathways Involved in Macrophage Inflammation Induced by Hypervirulent Klebsiella pneumoniae Infection.","authors":"Jinli Bi, Xin Huang, Liqin Meng, Jinghan Li, Yu He, Jing Jin, Taijie Li","doi":"10.1007/s00284-025-04481-x","DOIUrl":"10.1007/s00284-025-04481-x","url":null,"abstract":"Hypervirulent Klebsiella pneumoniae (hvKP) possesses multiple virulence factors and causes severe infections with elevated mortality rates. It induces a strong inflammatory response in the host, with macrophages playing a key role in defense and inflammation. However, the signaling pathways of macrophages involved in response to hvKP infection remain unclear. In this study, we developed a stable hvKP infection model in differentiated THP-1 macrophages, confirmed by CFU assay and LDH cytotoxicity assay, and subsequently performed transcriptomic profiling analysis of hvKP-infected cells to characterize infection-induced gene expression changes. Pathway and differential gene correlation network analyses identified 14 potential regulatory genes primarily involved in inflammation. These changes in gene expression were further validated by Reverse transcription quantitative PCR (RT-qPCR). KEGG and GO analyses revealed several key signaling pathways involved in inflammation and immune response, particularly the TNF, IL-17, NF-κB, and JAK-STAT pathways. Further analysis indicated that the NF-κB signaling pathway plays a central role in the inflammatory response to hvKP infection, whereas the TNF and IL-17 pathways act synergistically to upregulate pro-inflammatory cytokines and chemokines. Western blot analysis confirmed the expression of TRAF6 and NF-κB p65 protein during hvKP infection. This study provides valuable insights into the molecular mechanisms of macrophage responses to hvKP, offering potential targets for therapeutic intervention.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 10","pages":"492"},"PeriodicalIF":2.6,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145005921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unlocking the Secrets: Causal Associations Between Blood Metabolites and Aspergillosis. 揭开秘密：血液代谢物与曲霉病之间的因果关系。

IF 2.6 3区生物学

Current Microbiology Pub Date : 2025-09-04 DOI: 10.1007/s00284-025-04465-x

Youzhen Ma, Wenlong Du

{"title":"Unlocking the Secrets: Causal Associations Between Blood Metabolites and Aspergillosis.","authors":"Youzhen Ma, Wenlong Du","doi":"10.1007/s00284-025-04465-x","DOIUrl":"10.1007/s00284-025-04465-x","url":null,"abstract":"Aspergillosis includes a range of illnesses caused by Aspergillus species, primarily affecting individuals with weakened immune systems. Blood metabolites are gaining attention as potential biomarkers for diagnosing and managing diseases, but their causal role in aspergillosis risk remains unclear. This study used Mendelian randomization (MR) to explore potential causal associations between blood metabolites, their ratios, and aspergillosis risk. Single-nucleotide polymorphisms (SNPs) associated with metabolites were selected as instrumental variables. The primary analysis was conducted using the inverse variance weighted (IVW) method, with sensitivity analyses to ensure robustness. The study identified several metabolites and ratios significantly associated with aspergillosis risk. Elevated levels of 2-linoleoylglycerol, palmitoleate, serotonin, and 3-indoxyl sulfate were linked to increased risk, while higher levels of myristate, 1-methylhistidine, and theobromine were associated with reduced risk. Findings were validated using a secondary dataset, and reverse MR analysis confirmed the directionality from metabolites to disease. These results shed light on the metabolic underpinnings of aspergillosis and may inform future research on diagnostic and therapeutic strategies.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 10","pages":"490"},"PeriodicalIF":2.6,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144991746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Two Isolates From Plants Cultivated in Red Lateritic Soils are Good Candidates for the Development of Microbial Biostimulants. 从红土栽培植物中分离出的两种菌株是开发微生物刺激素的良好候选者。

IF 2.6 3区生物学

Current Microbiology Pub Date : 2025-09-02 DOI: 10.1007/s00284-025-04452-2

Daniela A Vallejo, Julia E García, Guillermo A Maroniche, Carlos F Piccinetti, Mariana L Puente, Esteban J Rubio, María D Groppa

{"title":"Two Isolates From Plants Cultivated in Red Lateritic Soils are Good Candidates for the Development of Microbial Biostimulants.","authors":"Daniela A Vallejo, Julia E García, Guillermo A Maroniche, Carlos F Piccinetti, Mariana L Puente, Esteban J Rubio, María D Groppa","doi":"10.1007/s00284-025-04452-2","DOIUrl":"10.1007/s00284-025-04452-2","url":null,"abstract":"Phosphorus is essential for plant growth, yet its availability in soils is extremely limited, with less than 0.1% accessible to plants. Though phosphate fertilizers are frequently employed to address this deficiency, a significant portion of the applied phosphorus is quickly fixed and unavailable to plants. Thus, the use of plant growth-promoting bacteria (PGPB) capable of solubilizing insoluble phosphate is an increasingly accepted eco-friendly agricultural practice to enhance phosphorus availability. This study aimed to isolate native phosphorus-solubilizing bacteria from lateritic and sandy soils of Misiones and Corrientes provinces (Argentina) and to study their plant growth-promoting attributes. Among the 17 isolates obtained following a selective protocol, two isolates, here named BVP24 and MMBR01, exhibited the highest phosphate solubilization activity (greater than other reported microorganisms) and produced substantial amounts of indole acetic acid. Both isolates were identified as Priestia megaterium via MALDI-TOF analysis and inhibited in vitro Fusarium graminearum growth. MMBR01 additionally inhibited Bipolaris sorokiniana growth. A dose of 1 × 106 CFU per seed was the most efficient to promote root dry weight in maize seedlings. Based on their plant growth-promoting capacities, these native strains emerge as potential biofertilizers, offering an alternative to synthetic phosphate fertilizers and contributing to more sustainable phosphorus management.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 10","pages":"488"},"PeriodicalIF":2.6,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144946860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Degradation of Resistant α-1,4-glucan by Vaginal Gardnerella Species is Associated with Bacterial Vaginosis. 阴道加德纳菌降解耐药α-1,4-葡聚糖与细菌性阴道病有关

IF 2.6 3区生物学

Current Microbiology Pub Date : 2025-09-02 DOI: 10.1007/s00284-025-04459-9

Rosanne Hertzberger, Sara Morselli, Sara Botschuijver, Lisa Himschoot, Leon Steenbergen, Sylvia Bruisten, Warren Lewis, Piet Cools, Remco Kort

{"title":"Degradation of Resistant α-1,4-glucan by Vaginal Gardnerella Species is Associated with Bacterial Vaginosis.","authors":"Rosanne Hertzberger, Sara Morselli, Sara Botschuijver, Lisa Himschoot, Leon Steenbergen, Sylvia Bruisten, Warren Lewis, Piet Cools, Remco Kort","doi":"10.1007/s00284-025-04459-9","DOIUrl":"10.1007/s00284-025-04459-9","url":null,"abstract":"This study investigates the degradation of resistant α-1,4-glucan by vaginal bacterial species, with a focus on Gardnerella spp., to elucidate its role in bacterial vaginosis (BV). The ability of Gardnerella vaginalis, Gardnerella swidsinskii, Gardnerella leopoldii, Gardnerella piotii, Lactobacillus iners, and Lactobacillus crispatus was assessed to metabolize an ungelatinized, labeled form of raw amylose, a degradation-resistant α-1,4-glucan. The enzymatic activity of these species was evaluated in vitro, and its association with BV was examined in vaginal swabs. Gardnerella vaginalis, G. swidsinskii, and G. leopoldii demonstrated the best ability to degrade resistant α-1,4-glucan in vitro. Unlike the cell-bound, S-layer-associated glycogen-degrading activity in L. crispatus, this α-glucosidase activity in Gardnerella was also extracellular, but not cell-bound and not repressed by glucose. Vaginal swabs showing high rates of resistant α-1,4-glucan degradation activity were associated with BV, particularly in the concurrent presence of G. leopoldii, G. swidsinskii, and G. vaginalis. These findings suggest a role of α-1,4-glucan degradation in BV pathogenesis mediated by Gardnerella species. The results indicate the potential of targeting bacterial amylase activity as therapeutic strategy for BV prevention and treatment.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 10","pages":"487"},"PeriodicalIF":2.6,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12405377/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144946458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural Dynamics of the Skin-Associated Microbiome of the Sea Cucumber Holothuria scabra During Integument Ulceration and Recovery. 海参疮痂Holothuria痂皮溃烂与恢复过程中皮肤相关微生物群的结构动力学。

IF 2.6 3区生物学

Current Microbiology Pub Date : 2025-09-02 DOI: 10.1007/s00284-025-04475-9

Yuanqiu He, Bovern Suchart Arromrak, Juan Diego Gaitan-Espitia

{"title":"Structural Dynamics of the Skin-Associated Microbiome of the Sea Cucumber Holothuria scabra During Integument Ulceration and Recovery.","authors":"Yuanqiu He, Bovern Suchart Arromrak, Juan Diego Gaitan-Espitia","doi":"10.1007/s00284-025-04475-9","DOIUrl":"10.1007/s00284-025-04475-9","url":null,"abstract":"Sea cucumbers are highly valued commercial species in the seafood industry. However, their hatchery and commercialization are constrained by the high mortalities experienced by these animals driven by environmental changes and physiological stress. Under such conditions, functional alterations in their immune system can induce integument ulcers that are later infected by opportunistic pathogens. Sea cucumbers, however, can display defense mechanisms that enable self-healing and recovery. It has been hypothesized that such mechanisms are assisted by changes in the skin-associated microbial community, enhancing the resilience of infected animal hosts. Here, we investigated the dynamics of the skin-associated microbiomes during infection and recovery of the tropical sea cucumber Holothuria scabra. Our findings reveal that the acclimation process for both, the host and the microbiome occurs simultaneously, with the latter gradually resembling the surrounding water environment after one month. The gradual healing of the sea cucumber skin and the structural changes in the associated microbiome were concurrent to the decline of pathogenic bacteria and the increase in the abundance of the family Rhodobacteraceae. The enrichment of some members from this group (genus Ruegeria) suggests a potential beneficial role in facilitating sea cucumber host recovery and acclimation. Our study provides valuable insights into the dynamics of the skin microbiome in sea cucumbers, highlighting the key role of this organismal trait for the resilience and overall health of the sea cucumber host.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 10","pages":"489"},"PeriodicalIF":2.6,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12405312/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144946826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacterial Extracellular Vesicles: Emerging Regulators in the Gut-Organ Axis and Prospective Biomedical Applications. 细菌细胞外囊泡：肠-器官轴的新兴调节因子和未来的生物医学应用。

IF 2.6 3区生物学

Current Microbiology Pub Date : 2025-09-01 DOI: 10.1007/s00284-025-04474-w

Bingqi Zhang, Mingyan Liu, Lingxin Jiang, Ziqiu Chen, Wei Tian, Kaidi Li, Bin Yu, Wenlan Zhang, Sirui Li, Yuzhu Zhou, Huhu Li, Yilan Xu, Yongna Dai

引用次数: 0

The Potential of Using Phosphate-Solubilizing Bacteria as a Controlled-Release Biofertilizer Under Salt Stress Conditions. 盐胁迫条件下磷酸盐增溶菌作为控释生物肥料的潜力。

IF 2.6 3区生物学

Current Microbiology Pub Date : 2025-08-31 DOI: 10.1007/s00284-025-04470-0

Mai Thi Ngoc Dinh, Van Thi Nguyen, Dat Van Dinh

{"title":"The Potential of Using Phosphate-Solubilizing Bacteria as a Controlled-Release Biofertilizer Under Salt Stress Conditions.","authors":"Mai Thi Ngoc Dinh, Van Thi Nguyen, Dat Van Dinh","doi":"10.1007/s00284-025-04470-0","DOIUrl":"10.1007/s00284-025-04470-0","url":null,"abstract":"Phosphorus deficiency in saline soils, despite high total phosphorus content, limits crop productivity due to low bioavailability. This study aimed to evaluate the phosphate-solubilizing potential and salt stress responses of Priestia megaterium PN18, a bacterium isolated from saline-affected soil, and to assess its suitability as a biofertilizer through cell encapsulation. PN18 was examined for biofilm formation, exopolysaccharide (EPS) production, and sodium uptake under NaCl concentrations ranging from 0.0 to 2.0 mol L-1. Results showed that biofilm formation decreased with increasing salinity, whereas EPS production increased, peaking at 1.2 mol L-1, in correlation with sodium uptake. Capsules formulated with 0.6% sodium alginate and 2% CaCl₂ had an average diameter of 2.81 mm and maintained phosphate-solubilizing activity under high salinity, with only a slight decline at 2.0 mol L-1 NaCl. While free PN18 exhibited a higher maximum solubilization (386 mg L-1) than encapsulated PN18 (292 mg L-1), its efficiency sharply declined above 0.4 mol L-1 NaCl. These findings highlight the potential of PN18 capsules as a controlled-release biofertilizer to improve phosphorus availability and soil fertility in saline environments.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 10","pages":"485"},"PeriodicalIF":2.6,"publicationDate":"2025-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144946851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated Microbiome Data Analysis Reveals Potential Pneumonia Microbial Biomarkers in ICU Patients: A Machine Learning Approach. 综合微生物组数据分析揭示了ICU患者潜在的肺炎微生物生物标志物：机器学习方法。

IF 2.6 3区生物学

Current Microbiology Pub Date : 2025-08-30 DOI: 10.1007/s00284-025-04464-y

Pownraj Brindangnanam, Mohane Selvaraj Coumar

{"title":"Integrated Microbiome Data Analysis Reveals Potential Pneumonia Microbial Biomarkers in ICU Patients: A Machine Learning Approach.","authors":"Pownraj Brindangnanam, Mohane Selvaraj Coumar","doi":"10.1007/s00284-025-04464-y","DOIUrl":"10.1007/s00284-025-04464-y","url":null,"abstract":"The human microbiome is pivotal in maintaining health and managing diseases. By examining the core microbiome in intensive care units (ICU) patients with pneumonia, we can gain valuable insights into the microbial communities associated with disease conditions. Pneumonia is the second most common infection in ICU settings, and recent research has highlighted the significance of endotracheal aspirate (ETA) microbiota in influencing pneumonia. Analysis of 16S rRNA sequencing data from lung microbiota of ICU patients revealed Pseudomonas as a key microbial biomarker, with machine learning model (xgbTree) achieving high predictive accuracy (prAUC: 0.98 and 0.7 log loss). Functional profile analysis revealed that the ATP-binding cassette (ABC) transporters and tetracycline-resistant ribosomal protection (Tet RPPs) proteins were possible molecular biomarkers that can be targeted to address the abundant pathogenic microbiome in pneumonia patients. These findings provide critical insights into pneumonia-specific microbiome signatures, highlighting Pseudomonas as a diagnostic marker and resistance-associated functional pathways as potential intervention targets. This study contributes to the development of precision medicine strategies for pneumonia management in ICU settings.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 10","pages":"483"},"PeriodicalIF":2.6,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144946772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preliminary Detection of Bats-associated Influenza A virus from Pakistan. 初步发现来自巴基斯坦的与蝙蝠有关的甲型流感病毒。

IF 2.6 3区生物学

Current Microbiology Pub Date : 2025-08-30 DOI: 10.1007/s00284-025-04467-9

Sidra Rahman, Syed Ahsan Shahid, Hassan Ayaz, Sajid Ali Shah, Muhammad Ali

引用次数: 0