Current Microbiology最新文献_第8页

Distinct Communities of Bacteria and Unicellular Eukaryotes in the Different Water Masses of Cretan Passage Water Column (Eastern Mediterranean Sea). 克里特海峡水柱（东地中海）不同水质中细菌和单细胞真核生物群落的差异。

IF 2.3 3区生物学

Current Microbiology Pub Date : 2024-09-28 DOI: 10.1007/s00284-024-03906-3

Georgia Charalampous, Konstantinos A Kormas, Eleftheria Antoniou, Nicolas Kalogerakis, Evangelia Gontikaki

{"title":"Distinct Communities of Bacteria and Unicellular Eukaryotes in the Different Water Masses of Cretan Passage Water Column (Eastern Mediterranean Sea).","authors":"Georgia Charalampous, Konstantinos A Kormas, Eleftheria Antoniou, Nicolas Kalogerakis, Evangelia Gontikaki","doi":"10.1007/s00284-024-03906-3","DOIUrl":"10.1007/s00284-024-03906-3","url":null,"abstract":"Elucidating marine microbiota diversity and dynamics holds significant importance due to their role in maintaining vital ecosystem functions and services including climate regulation. This work aims to contribute in the understanding of microbial ecology and networking in one of the world's most understudied marine regions, the Eastern Mediterranean Sea. High-throughput 16S and 18S rRNA gene sequencing analysis was applied to study the diversity of bacteria and unicellular eukaryotes in the different water masses of the Cretan Passage during two seasonally-different sampling expeditions. We assumed that microbial associations differ between the surface and deepwater masses and created co-occurrence networks to evaluate this hypothesis. Our results unveiled vertical variations in both bacterial and unicellular eukaryotic diversity with species fluctuations indicative of seasonality being recorded in the surface water mass. Heterotrophic taxa and grazers related to organic matter degradation and nutrient cycling were enriched in the deepest water layers. Moreover, surface waters presented a higher number of microbial associations indicating abundant ecological niches compared to the deepest layer, possibly related to the lack of bottom-up resources in the oligotrophic deep ocean. Overall, our data provide insight in a heavily stressed, yet underexplored, marine area that requires further research to unravel the ecological roles of marine microbes. To our knowledge, this is the first study that combines molecular biology tools to provide data on both planktic prokaryotes and unicellular eukaryotes across the different water masses in this marine region of the Eastern Mediterranean basin.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142343691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lelliottia wanjuensis sp. nov. Isolated from Korean Lettuce in Wanju, South Korea. Lelliottia wanjuensis sp.

IF 2.3 3区生物学

Current Microbiology Pub Date : 2024-09-28 DOI: 10.1007/s00284-024-03911-6

Jiyeon Lee, Eewon Tai, Sunyoung Jeong, Ile Kim, Bo-Eun Kim, Myeong-In Jeong, Kwang-Kyo Oh, Natalia Biere, Charles M A P Franz, Gyu-Sung Cho

{"title":"Lelliottia wanjuensis sp. nov. Isolated from Korean Lettuce in Wanju, South Korea.","authors":"Jiyeon Lee, Eewon Tai, Sunyoung Jeong, Ile Kim, Bo-Eun Kim, Myeong-In Jeong, Kwang-Kyo Oh, Natalia Biere, Charles M A P Franz, Gyu-Sung Cho","doi":"10.1007/s00284-024-03911-6","DOIUrl":"10.1007/s00284-024-03911-6","url":null,"abstract":"Ten Gram stain-negative, oxidase-negative, catalase-positive, rod-shaped strains were isolated from lettuce in the city Wanju in South Korea. Comparative 16S rRNA gene analysis and multilocus sequence analysis indicated that the strains grouped closely together with all other Lelliottia (L.)-type strains. The average nucleotide identity (ANI) comparisons of the isolates showed a high relationship between the strains, as the ANI values of all strains ranged from 91.01 to 91.31% when compared to the L. jeotgali-type strain PFL01T. All isolates showed the highest genomic DNA sequence similarity to the L. jeotgali PFL01T strain at 43.3% when compared with digital DNA-DNA hybridization (dDDH). Strain V104_15T of the proposed novel Lelliottia species showed 91.2% and 43.3% similarity with the most closely related L. jeotgali PFL01T-type strain in the ANI and dDDH comparisons, respectively. Strain V104_15T could not grow at 45 °C and 7% NaCl, while L. jeotgali PFL01T strain could grow at both these conditions. Strain V104_15T showed ß-glucosidase activity but not α-glucosidase activity, while the L. jeotgali PFL01T strain was α-glucosidase positive but ß-glucosidase negative. The major cellular fatty acids of strain V104_15T were C16:0 and cyclo-C17:0 including summed features. The mol % G + C content of the genomic DNA of strain V104_15T was 55.74%. Phenotypic and biochemical characteristics, as well as the phylogenomic analysis indicated that the strain V104_15T represents a novel species of the genus Lelliottia, for which the name Lelliottia wanjuensis sp. nov. is proposed. The type strain is V104_15T (= LMG 32996 T = DSM 115585 T).","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11438706/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142343708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anti-Magnaporthe oryzae Activity of Streptomyces bikiniensis HD-087 In Vitro and Bioinformatics Analysis of Polyketide Synthase Gene pksL. 比基尼链霉菌（Streptomyces bikiniensis）HD-087 的体外抗马齿苋活性和多酮合成酶基因 pksL 的生物信息学分析

IF 2.3 3区生物学

Current Microbiology Pub Date : 2024-09-28 DOI: 10.1007/s00284-024-03898-0

Jiahan Gang, Yuan Ping, Chunmei Du

{"title":"Anti-Magnaporthe oryzae Activity of Streptomyces bikiniensis HD-087 In Vitro and Bioinformatics Analysis of Polyketide Synthase Gene pksL.","authors":"Jiahan Gang, Yuan Ping, Chunmei Du","doi":"10.1007/s00284-024-03898-0","DOIUrl":"10.1007/s00284-024-03898-0","url":null,"abstract":"Streptomyces bikiniensis HD-087 is capable of synthesizing various antimicrobial substances to counter the detrimental effects of hazardous microorganisms. To elucidate whether it produces polyketide antibiotics and the synthesis mechanism of antibiotic substances, the metabolites and related genes of S. bikiniensis HD-087 were analyzed through LC-MS, anti-Magnaporthe oryzae activity detection, and bioinformatics approaches. The result indicated that the strain HD-087 could produce erythromycin, a polyketide antibiotic. The inhibitory zones of the fermentation supernatant of strain HD-087 and methanol solution of erythromycin extract against M. oryzae were 40.84 ± 0.68 mm and 33.18 ± 0.81 mm, respectively. The IC50 value of erythromycin extract for inhibiting spore germination of erythromycin extract was 220.43 μg/mL. There are two polyketide synthesis gene clusters in the genome of strain HD-087, namely t1pks-nrps and t3pks-lantipeptide-t1pks-nrps. The key gene pksL in the t3pks-lantipeptide-t1pks-nrps gene cluster was predicted. The results suggested that it encodes a stable, hydrophilic, and acidic protein, mainly composed of α-helix and random coil. The PksL protein contains dehydrogenase (DH), ketone reductase (KR), acyl carrier protein (ACP), and ketone synthase (KS) domains. Moreover, it can form interaction networks with 11 proteins containing domains, such as polyketide synthase and ACP synthase. The molecular docking between PksL and acetyl-CoA is stable and strong, suggesting that PksL protein could catalyze the synthesis of polyketides with CoA as a substrate. This study provides a theoretical basis for further exploring the polyketides synthesis mechanism and developing antifungal metabolites in S. bikiniensis HD-087.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142343689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production of Tobacco Etch Virus Protease (TEV) Expressed in the Endotoxin-Free Bacillus subtilis and Its Application. 用无内毒素枯草芽孢杆菌表达的烟草蚀刻病毒蛋白酶（TEV）的生产及其应用。

IF 2.3 3区生物学

Current Microbiology Pub Date : 2024-09-26 DOI: 10.1007/s00284-024-03907-2

Vuong Duong Le, Trang Thi Phuong Phan, Hoang Duc Nguyen

{"title":"Production of Tobacco Etch Virus Protease (TEV) Expressed in the Endotoxin-Free Bacillus subtilis and Its Application.","authors":"Vuong Duong Le, Trang Thi Phuong Phan, Hoang Duc Nguyen","doi":"10.1007/s00284-024-03907-2","DOIUrl":"10.1007/s00284-024-03907-2","url":null,"abstract":"Tobacco Etch virus (TEV) protease is one of the most common tools for removing fusion tags, but no study has shown that TEV can be expressed at high levels in the GRAS host strain Bacillus subtilis and purified for further application. In this study, the fusion protein BsLysSN-TEV C/S-His-TEV consisting of a fusion tag, N-terminal domain of a lysyl-tRNA synthetase (BsLysSN) coded by B. subtilis lysS gene, placed at the N-terminus followed by an endoprotease TEV cleavage site and then the expression of this fusion protein in the cytoplasm of B. subtilis was investigated. The SDS-PAGE and Western-blot analysis demonstrated that His-TEV was overexpressed under the induction of IPTG. This result infers that His-TEV protease showed promising activity in the B. subtilis cytoplasm by the cleavage of the fusion protein. These cleavage products could be purified using the Ni-NTA column, which effectively cleaved the purified recombinant protein substrate, which can be applied in the protein purification process to remove the fusion tag. Significantly, since both His-TEV protease and the fusion recombinant protein substrate are expressed in the endotoxin-free host strain, the tag removal and purified product should be theoretically endotoxin-free, which could be a promising approach for producing therapeutic proteins and also for other relevant biomedical applications.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142343710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pseudoplagiostoma humilis sp. nov., a New Fungal Species Causing Shoot Blight and Dieback in Anacardium humile in Brazil. Pseudoplagiostoma humilis sp.

IF 2.3 3区生物学

Current Microbiology Pub Date : 2024-09-26 DOI: 10.1007/s00284-024-03894-4

Luiz Pedro Pereira Magalhães, Nilza de Lima Pereira Sales, Patrícia Doerl Barroso, Rildo Alexandre Fernandes da Silva, Danilo Batista Pinho, José Cola Zanuncio, André Costa da Silva

{"title":"Pseudoplagiostoma humilis sp. nov., a New Fungal Species Causing Shoot Blight and Dieback in Anacardium humile in Brazil.","authors":"Luiz Pedro Pereira Magalhães, Nilza de Lima Pereira Sales, Patrícia Doerl Barroso, Rildo Alexandre Fernandes da Silva, Danilo Batista Pinho, José Cola Zanuncio, André Costa da Silva","doi":"10.1007/s00284-024-03894-4","DOIUrl":"10.1007/s00284-024-03894-4","url":null,"abstract":"The cajuzinho do cerrado (Anacardium humile-Anacardiaceae), a shrub species native to Brazil, is harvested for multiple uses in food and medicine. Members of a harvesting community, near the municipality of Bonito de Minas, Minas Gerais state, Brazil reported characteristic symptoms of shoot blight and dieback reducing pseudofruit and seed production by this plant. This study aimed to identify the etiological agent of this disease. Two fungal isolates were obtained from symptomatic leaf samples and morphologically and molecularly characterized. The fungus was identified, based on morphological analyses, as a probable new species of Pseudoplagiostoma. Phylogenetic analyses based on a combination of DNA sequence data (nuc rDNA ITS1-5.8S-ITS2 region, tef1-α and tub2), confirmed this hypothesis. The isolates obtained were allocated to a distinct, well-supported clade (IB = 0.99, ML = 100%), placed as a unique lineage here proposed as a new species named Pseudoplagiostoma humilis. The pathogenicity test confirmed that this new species was the causal agent of shoot blight and dieback on A. humile. This is the fourteenth Pseudoplagiostoma species reported in the world and the third in Brazil.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142343711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PGPR: Key to Enhancing Crop Productivity and Achieving Sustainable Agriculture. PGPR：提高作物生产力和实现可持续农业的关键。

IF 2.3 3区生物学

Current Microbiology Pub Date : 2024-09-26 DOI: 10.1007/s00284-024-03893-5

Shidong He, Lingli Li, Minghao Lv, Rongxin Wang, Lujun Wang, Shaowei Yu, Zheng Gao, Xiang Li

{"title":"PGPR: Key to Enhancing Crop Productivity and Achieving Sustainable Agriculture.","authors":"Shidong He, Lingli Li, Minghao Lv, Rongxin Wang, Lujun Wang, Shaowei Yu, Zheng Gao, Xiang Li","doi":"10.1007/s00284-024-03893-5","DOIUrl":"10.1007/s00284-024-03893-5","url":null,"abstract":"Due to the burgeoning global population and the advancement of economies, coupled with human activities leading to the degradation of soil ecosystems and the depletion of non-renewable resources, concerns have arisen regarding food security and human survival. In order to address these adverse impacts, the spotlight has been cast upon plant growth-promoting rhizobacteria (PGPR), driven by a strong environmental consciousness. PGPR possesses the capability to foster plant growth and amplify crop yield through both direct and indirect mechanisms. By expediting plant growth, augmenting nutrient assimilation, heightening crop yield and caliber, and fortifying stress resilience, the application of PGPR can mitigate reliance on chemical fertilizers and pesticides while diminishing ecological perils. This exposition delves into the function of PGPR in modulating plant hormones, fostering nutrient solubilization, and fortifying plant resistance against biotic and abiotic stressors. This review offers valuable insights into the intricate interplay between PGPR and plants, elucidating uncertainties ripe for further investigation. Profound comprehension and judicious utilization of PGPR are indispensable for attaining sustainable agricultural progression, making substantial contributions to resolving the conundrums of global food security and environmental conservation.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142343709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization and Promising in vitro Antiherpetic Effect of Galactomannan from Delonix regia Seeds. 德洛尼西娅种子中的半乳甘露聚糖的特征和体外抗带状疱疹的良好效果

IF 2.3 3区生物学

Current Microbiology Pub Date : 2024-09-25 DOI: 10.1007/s00284-024-03903-6

Jonatas Fernando Cavalini de Moraes, Daniele Zendrini Rechenchoski, André Luiz Dyna, Arcelina Pacheco Cunha, Nágila Maria Pontes Silva Ricardo, Silvana Silveira de Farias, Selene Maia de Morais, Lucy Megumi Yamauchi, Ligia Carla Faccin-Galhardi

{"title":"Characterization and Promising in vitro Antiherpetic Effect of Galactomannan from Delonix regia Seeds.","authors":"Jonatas Fernando Cavalini de Moraes, Daniele Zendrini Rechenchoski, André Luiz Dyna, Arcelina Pacheco Cunha, Nágila Maria Pontes Silva Ricardo, Silvana Silveira de Farias, Selene Maia de Morais, Lucy Megumi Yamauchi, Ligia Carla Faccin-Galhardi","doi":"10.1007/s00284-024-03903-6","DOIUrl":"10.1007/s00284-024-03903-6","url":null,"abstract":"Herpes simplex virus (HSV) infections can occur throughout life, thereby allowing transmission to new hosts, with an impact on public health. Acyclovir remains the treatment of choice for these infections; however, an increase in resistant strains in recent years has been observed. In this study, the activity of a native Delonix regia galactomannan (NDr) against HSV-1 was investigated in vitro. NDr was characterized using infrared spectroscopy and NMR. Evaluation of cytotoxicity and the antiviral effect was determined, respectively, by MTT and plaque reduction assays. The NDr concentrations that inhibited cell viability (CC50) and viral infection (IC50) by 50% were above 2000 and 64 μg/mL, respectively. Thus, the polysaccharide showed a high selectivity index (> 31.25). When NDr was added at different stages of HSV-1 replication, a strong inhibitory effect was found by direct interaction with the virus (71-67%, virucidal effect) or previously with the cell, 6 h before infection (99.8-68.4%, prophylactic effect) at concentrations from 200 to 50 μg/mL. NDr showed similar effects in prophylactic 1 h (52%) and adsorption inhibition (55%) assays at 200 μg/mL. A reduction in the antiherpetic effect was observed after infection. These results suggest that NDr is effective in the early stages of HSV-1 infection and is a promising agent for controlling herpetic infections.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142343690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lacticaseibacillus jixiensis sp. nov., Isolated from Traditional Chinese Pickle. 从中国传统泡菜中分离出的吉溪乳酸菌（Lacticaseibacillus jixiensis sp.

IF 2.3 3区生物学

Current Microbiology Pub Date : 2024-09-24 DOI: 10.1007/s00284-024-03902-7

Si-Tong Song, Chun Yan Li, Chun Tao Gu

引用次数: 0

Inflammatory Modulation Effects of Probiotics: A Safe and Promising Modulator for Cancer Prevention. 益生菌的炎症调节作用：一种安全且有望预防癌症的调节剂

IF 2.3 3区生物学

Current Microbiology Pub Date : 2024-09-23 DOI: 10.1007/s00284-024-03901-8

Mehran Mahooti, Elahe Abdolalipour, Samira Sanami, Davood Zare

{"title":"Inflammatory Modulation Effects of Probiotics: A Safe and Promising Modulator for Cancer Prevention.","authors":"Mehran Mahooti, Elahe Abdolalipour, Samira Sanami, Davood Zare","doi":"10.1007/s00284-024-03901-8","DOIUrl":"10.1007/s00284-024-03901-8","url":null,"abstract":"Chronic inflammation is the gate of many human illnesses and happens when the immune system is unable to suppress external attacks in the correct form. Nonetheless, the gut microbiome plays a pivotal role in keeping homeostasis in the human body and preventing inflammation. Imbalanced microbiota and many diseases can result in inflammation, which when not taken seriously, can be turned into chronic ones and ultimately lead to serious diseases such as cancer. One approach to maintaining hemostasis in the human body is consumption of probiotics as a supplement. Probiotics impact the immune functions of dendritic cells (DCs), T cells, and B cells in the gut-associated lymphoid tissue by inducing the secretion of an array of cytokines. They activate the innate immune response through their microbial-associated molecular pattern, and this activation is followed by multiple cytokine secretion and adaptive elicitation that mitigates pro-inflammatory expression levels and tumor incidence. Thus, according to several studies showing the benefit of probiotics application, alone or in combination with other agents, to induce potent immune responses in individuals against some inflammatory disorders and distinct types of cancers, this review is devoted to surveying the role of probiotics and the modulation of inflammation in some cancer models.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142282052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Metagenomic Analysis of Seed Endobiome of Domesticated and Wild Finger Millet Species (Eleusine spp.): Unveiling Microbial Diversity and Composition. 驯化和野生纤小粟（Eleusine spp.）种子内生物组的元基因组比较分析：揭示微生物的多样性和组成。

IF 2.3 3区生物学

Current Microbiology Pub Date : 2024-09-23 DOI: 10.1007/s00284-024-03891-7

M Hemapriya, K N Nataraja, T S Suryanarayanan, R Uma Shaanker

{"title":"Comparative Metagenomic Analysis of Seed Endobiome of Domesticated and Wild Finger Millet Species (Eleusine spp.): Unveiling Microbial Diversity and Composition.","authors":"M Hemapriya, K N Nataraja, T S Suryanarayanan, R Uma Shaanker","doi":"10.1007/s00284-024-03891-7","DOIUrl":"10.1007/s00284-024-03891-7","url":null,"abstract":"Domestication, which involves selective breeding, modern agricultural practices, and specific growing conditions, can influence the microbial and endophytic communities in crop plants. In this study, we examined the microbial diversity and community composition in the seeds of wild and domesticated finger millet species. We employed a metagenomic approach to investigate the seed microbial diversity and community composition of wild (Eleusine africana) and domesticated finger millet species (Eleusine coracana (L.) Gaertn) grown in the same habitat. While our findings indicated no significant change in seed endobiome diversity due to domestication, there were differences in microbial community composition between wild and domesticated species. Seeds of domesticated species had higher relative abundance of certain bacterial genera including Helicobacter, Akkermansia, Streptococcus, Bacteroides, and Pseudomonas, whereas seeds of wild species had higher relative abundance of unclassified Streptophyta. The seed-associated microbiota also varied among domesticated finger millet accessions. Co-occurrence network analysis revealed a strong relationship between bacteria and fungi in domesticated compared to wild species. We discuss the results obtained in the larger context of the importance of seed endobiome and how domestication processes in crop plants may have impacted the seed endobiome diversity, composition, and function compared to their wild counterparts.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142307303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0