Terrimonas alba sp. nov., an Ochratoxin A-Degrading Strain Isolated from Lakeside Soil.

Abstract

A Gram-negative, aerobic, rod-shaped, and non-motile bacterium, designated strain R1^T, was isolated from lakeside soil in Nanchang, Jiangxi Province, China. Growth was observed at 15-37 °C (optimum 30 °C), pH 6-8 (optimum pH 7) and salinity of 0-1.0% (w/v; optimum 0.5%). Strain R1^T completely degraded 5 mg/L of ochratoxin A within 48 h of incubation. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain R1^T belongs to the genus Terrimonas, and showed 99% sequences similarity with Terrimonas pollutisoli H1YJ31^T. The draft genome of strain R1^T was approximately 6.2 Mb with a 41.5% G + C content. The average nucleotide identity and digital DNA-DNA hybridization values between strain R1^T and T. pollutisoli H1YJ31^T were 90.4% and 41.2%, respectively. The predominant fatty acids of strain R1^T were iso-C_15:0, summed feature 3 (C_16:1 ω6c and/or C_16:1 ω7c), iso-C_17:0 3-OH and iso-C_15:1 G. The major isoprenoid quinone was menaquinone 7 (MK-7). The major polar lipids of strain R1^T were phosphatidylethanolamine, phosphatidylglycerol, a phospholipid, two unidentified glycolipids, two unidentified aminophospholipids, and five unidentified polar lipids. Based on phenotypic, genotypic and phylogenetic evidence, strain R1^T represents a novel species in the genus Terrimonas, for which the name Terrimonas alba sp. nov. is proposed. The type strain is R1^T (= GDMCC 1.4673 ^T = JCM 37058 ^T = KCTC 102293 ^T = KACC 23743 ^T).