Oryzifoliimicrobium ureilyticus gen. nov., sp. nov., a Novel Phosphate-Solubilizing and Siderophore-Producing Bacterium Within the Family Rhizobiaceae.

Abstract

A Gram-stain-negative, rod-shaped, strictly aerobic bacterium strain SG148^T, within the family Rhizobiaceae, was isolated from rice leaves collected in Fujian Province, China. Phylogenetic analysis utilizing 16S rRNA gene sequences revealed that strain SG148^T displayed a high sequence similarity of 95.2-95.8% to the strains of closely related species of Shinella and Rhizobium. The highest digital DNA-DNA hybridization (dDDH), core-proteome average amino acid identity (cpAAI), and average nucleotide identity (ANI) values between strain SG148^T and related strains were estimated as 20.7%, 79.3%, and 73.7%, respectively. The predominant respiratory quinones in strain SG148^T were identified as Q-9 and Q-10, while major polar lipids included diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, and phosphatidylethanolamine. Additionally, strain SG148^T was capable of phosphate-solubilizing and siderophore-producing carrier, significantly promoting rice seedling growth. The DNA G + C content was determined to be 56.1%. Given its distinct phylogenetic and phenotypic characteristics, strain SG148^T is considered to represent a novel genus, for which the name Oryzifoliimicrobium ureilyticus gen. nov., sp. nov. is proposed. The type strain is SG148^T (= MCCC 1K08901^T = KCTC 8337^T).